第 7 章 付録
7.2 流束計算に用いた 2 重境界膜理論
111
初めに気-PDMS の境界であるガス相について計算する．フィックの法則より このガス相におけるNO流束は
𝑁€ = 𝐷€ 1
𝛿€ 𝑝 − 𝑝• (7-15)
となる．同様にPDMS相，PDMS-液の境界である液相について流束を求めると
𝑁Q = 𝐷Q 1
𝛿Q 𝐶&− 𝐶3 (7-16)
𝑁‚ = 𝐷‚ 1
𝛿‚ 𝐶‚− 𝐶 (7-17)
となる．ただし，pi，C2は平衡定数を用いて
Table 7-1 2 重境界膜理論に使用する記号の意味
𝑃• = 𝐸&𝐶& (7-18)
𝐶3 = 𝐸3𝐶‚ (7-19)
と表させる．定常状態ではNG = NM = NMであるか，(7-15)，(7-16)，(7-17)式より 流束Nは以下の式になる．
N =𝐷€
𝛿€ 𝑝 − 𝑝• =𝐷Q
𝛿Q 𝐶&− 𝐶3 =𝐷‚
𝛿‚ 𝐶‚− 𝐶 (7-20)
(7-20)式に(7-18)式と(7-19)式を代入すると
N = 𝐾E… 𝐶∗− 𝐶 (7-21) 1
𝐾E… = 𝛿€
𝐸&𝐸3𝐷€+ 𝛿Q
𝐸3𝐷Q+𝛿‚
𝐷‚ (7-22)
𝐶∗ = 𝑝
𝐸&𝐸3 (7-23)
となる．ここで，ガス相と液相は架空に設定した相であり，dGとdLはdMに比べ て小さいので，(7-22)式は以下のように近似できる．
𝐾E… = 𝐸3𝐷Q
𝛿Q (7-24)
したがって，NOの流束Nは次の式にまとめることができる．
N =𝐸3𝐷Q 𝛿Q
𝑝
𝐸&𝐸3− 𝐶 (7-25)
第4章で作製し用いたNO負荷装置における各係数値をTable 7-2に示す[72]．
113
定常状態になった時のC は第4章の(4-3)式から求められる．各 NO負荷にお けるCは，C0.5ppm = 2.15 × 10-9 mol / L，C5ppm = 19 × 10-9 mol / L，C25ppm = 0.06 × 10-6 mol / Lとなった．
これらの数値を用いて作製したNO負荷装置におけるNO流速を求められる．
Table 7-2 作製した NO 負荷装置における各係数
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発表目録
原著論文
1. Tateki SUMII, Ryosuke FUJITA, Kazuo TANISHITA, Susumu KUDO, “Effect of Flow Load on Hepatic Function in Co-Culture of Hepatocytes with Hepatic Stellate Cells and Endothelial Cells: Relationship between Hepatic Function and Nitric Oxide Concentration in vitro”, Journal of Biomechanical Science and Engineering, Vol. 7(2), 237-47, 2012
2. Tateki Sumii, Yohei Nakano, Takuma Abe, Kazuhiro Nakashima, Toshihiro Sera, Susumu Kudo, “Effect of nitric oxide on ammonia decomposition in static culture and under shear stress”, Journal of Biorheology, Vol. 30(1), 19-26, 2016
3. Tateki Sumii, Yohei Nakano, Takuma Abe, Kazuhiro Nakashima, Toshihiro Sera, Susumu Kudo, “The Effect of Nitric Oxide on Ammonia Decomposition in Co-cultures of Hepatocytes and Hepatic Stellate Cells”, In Vitro Cellular &
Developmental Biology - Animal, Vol. 52(6), 625-31, 2016
全文査読付国際学会
1. Tateki Sumii, Ryosuke Fujita, Kazuo Tanishita, Susumu Kudo, 「Flow and Nitric Oxide Increase Hepatic Function in Co-Culturing Hepatocytes with Hepatic Stellate Cells and Endothelial Cells」, MHS2011 & Micro-Nano Global COE, 2011年11月
アブストラクト査読付国際学会
1. Tateki SUMII, Ryosuke FUJITA, Kazuhiro NAKASHIMA, Susumu KUDO,
「Effect of Shear Stress on Migration of hepatic Stellate Cells」, ICBME2013, 2013 年12月
国際学会
1. Tateki Sumii, Ryosuke Fujita, Kazuo Tanishita, Susumu Kudo, 「Roles of Mechanical Stress and Nitric Oxide on Hepatic Function」 , The Sixth KAIST-Kyushu University Joint Workshop on Frontiers in Mechanical and Aerospace Engineering , 2012年9月
2. Tateki SUMII, Yohei NAKANO, Kazuhiro NAKASHIMA, Toshihiro SERA, Susumu KUDO, 「Research of relationship between nitric oxide and hepatic
123
function」, The 4th Japan-Switzerland Workshop on Biomechanics (JSB2014), 2014 年9月
3. Tateki SUMII, Yohei NAKANO, Kazuhiro NAKASHIMA, Toshihiro SERA, Susumu KUDO, 「Direct Load of Nitric Oxide Affect Hepatic Function」, The Seventh KAIST-Kyushu University Joint Workshop on Frontiers in Mechanical Engineering, 2014年9月
国内学会
1. 隅井干城，藤田陵佑，谷下一夫，工藤奨，「せん断応力負荷時における共培 養モデルでの肝細胞機能の評価」，日本生体医工学会関東支部若手研究発表 会2010，2010年11月
2. 隅井干城，藤田陵佑，谷下一夫，工藤奨，「肝細胞共培養モデルにおける肝 機能促進とNO濃度の関連性」，日本機械学会第22回バイオフロンティア講 演会，2011年10月
3. 隅井干城，藤田陵佑，谷下一夫，工藤奨，「肝細胞機能向上と NO 濃度の関 連性」，平成23年度日本生理人類学会研究奨励発表会，2011年12月
4. 隅井干城，「肝細胞機能向上における共培養と力学刺激の役割」，第9回博士 学生交流セミナー，2012年8月
5. 隅井干城，木村恭彰，島田知弥，土屋宏紀，工藤奨，「血管内皮細胞内 PKC αの局在変化におよぼす局所引張りの影響」，第36回日本バイオレオロジー 学会年会，2013年6月
6. 隅井干城，藤田陵佑，岩下洸，工藤奨，「肝星細胞の遊走における力学刺激 と受容体の関連性」，第36回日本バイオレオロジー学会年会，2013年6月 7. 隅井干城，藤田陵佑，岩下洸，工藤奨，「肝星細胞の遊走におけるせん断応
力の大きさと方向の影響」，日本生体医工学会生体医工学シンポジウム2013， 2013年9月
8. 隅井干城，藤田陵佑，岩下洸，工藤奨，「流れ方向とせん断応力の大きさが 肝星細胞遊走に与える影響」，第49回九大生体材料・力学研究会，2013年9 月
9. 隅井干城，阿部拓磨，中嶋和弘，世良俊博，工藤奨，「肝細胞機能における 一酸化窒素直接負荷の影響」，バイオメカニクス研究センター＆エレクトロ ニクス実装学会九州支部合同研究会，2014年2月
10. 隅井干城，阿部拓磨，中嶋和弘，世良俊博，工藤奨，「一酸化窒素直接負荷
による肝細胞機能への影響」，平成25年日本生理人類学会研究奨励会，2014 年2月
11. 隅井干城，阿部拓磨，中嶋和弘，世良俊博，工藤奨，「肝細胞機能に及ぼす 一酸化窒素の影響」，第37回日本バイオレオロジー学会年会，2014年6月
12. 隅井干城，「肝細胞機能向上における一酸化窒素とせん断応力の影響」，第12
回博士学生交流セミナー，2015年8月
13. 隅井干城，中野陽平，阿部拓磨，中嶋和弘，世良俊博，工藤奨，「一酸化窒 素直接負荷における共培養モデルでの肝細胞機能評価」，日本機械学会 2016 年次大会，2016年9月
受賞歴など
1. 2010 年 11 月 日本生体医工学会関東支部若手研究者発表会 2010 優秀発表 賞
2. 2011年12月 平成23年日本生理人類学会研究奨励発表会優秀発表賞 3. 2012年1月 日本機械学会若手優秀講演フェロー賞
4. 2012年8月 第9回博士学生交流セミナー優秀発表賞 5. 2014年6月 第37回日本バイオレオロジー学会学術奨励賞 6. 2015年8月 第12回博士学生交流セミナー優秀発表賞 7. 日本学術振興会特別研究員（DC2）2013年4月～2015年3月