岡山醫學會雜誌第四百三十四號大正十五年三月三十一日發行

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(1)岡山醫學會雜誌第四百三十四號大正十五年三月三十一日發行. OKAYAMA‑IGAKKAI‑ZASSHI Nr. 434, Marz. 31, 1926. 原 On. Adrenalinaemia. 著. resulting from. Central. Glycosuria,. as studiedby the Quantitative Change of Glycogen in the Milk‑spots. By. Dr. Yukio. Hamazaki.. From the PathologicalDepartment of Okayama Medical College. (Director:Prof.Oto Tamura) Receivedfor publication, November 25, 1925.. Contents. Ⅳ.. Introduction. Ⅰ. Ⅱ.. Ⅲ.. Renal. Ⅴ.. Method. Central. Ⅵ.. glycosuria. Piqure.. 2.. Diuretin‑injection.. Peripheral. Review. Ⅶ.. 1.. glycosuria.. Diuretin‑injection. followed. of literature. and. by. adrenalin.. discussion.. Conclusions. Bibliography.. glycosuria.. Introduction. Whether. or not central glycosuria. peripheral one, is a very important. is caused. question. which. by. adrenalinaemia. has been. taken. up. similarly to the by. many. investi‑. gators.. For thisproblem Andr'eMayer's work (1906)thatthe diabetic puncturemade after the removal experimentum nothing 1. of both the adrenals does not cause crucis.. However,. glycosuria,. this inference may. but a proof that the effect of diabetic puncture. not be. has. does. been. considered. as the. correct, since his result is not reach. directly the liver,.

(2) 248. Hamazaki‑On Adrenalinaemia resulting from CentralGlycosuria, etc.. but to. indirectly. by. determine. paper. how. entitled. preliminary diuretin, ject. the. supra‑renal. stimulation on. on. adrenalin. the. reaches. glycogen. changes. and. glands. the. of the of the. phloridzin.. Therefore, liver. from. milk‑spots. And. the. in. milk‑spots. in the. in the. it becomes glands.. the. present. In. omentum". cases. of. I shall. very the I. piqure. deal. desirable preceding. reported. and. with. in. injection. a. the. of. same. sub. detail. in. preceding. the. paper.. of the. milk‑spots. But. healthy. proportion;. and. this. way. in some. deposited. of. "Studies. Glycogen. 60%. way. by. adult. way. of. the. of. introduction. rabbits. healthy. glycogen. adult I. may. is present In. and. it is mostly. in the mesothelial. was. repeat in. (++):(+):(±):(‑)=1:1:1:2.. only in the milk‑spots,. in small quantity. rabbit. the. the. contained. fully the. statement. milk‑spots omentum. described. in. the. here.. in. the. glycogen. In. following is. always. in the histiocytic cells there. cells.. Ⅰ.. Methods.. The rabbitswere fed with "Tofukasu", residualof soya‑beancheese. Tissuesfrom the rabbitswere passed through graded alcoholsup to absoluteand were examined eitheras stretchedspecimens or as celloidin‑sectins. Best'spotassiumcarmine method was mostly used for glycogen‑stainand Langhan's iodinemethod and siva‑. reaction werealsomadeuseof. Blood‑sugar was determined by Kasamatsuand Hattori's method. The blood for sugar‑test was ed.. Glycosuria. obtained was. from. tested by. the auricular vein, keeping Nylander's. and. Ⅱ.. Trommel's. Central. the animal. in natural. condition. without. being. fasten. methods.. glycosuria.. 1. Piqure.. In 1854 and 1855 Cl.Bernarddiscoveredthatglycosuriais produced by means of punctureon the median lineofthe floorofthe fourthventricle bounded by the lines,one connectingthe originsofboth the auditorynervesand the other connectingthe origns of both the vagusesand he designatedthisregion"sugar‑centre". According to Ber nard and Eckhard, the stimulation producedby the puncturegoes downwards along the spinalto the first thoracic vertebraand reachesthe liverby way of the truncussymp athicusand the n. splanchnicus, thuscausingthe transformation of glycogen intosugr. intheliver.Blum (1901)observedthatthe injection of the extractof the supra‑real glandscausesa glycosuria and basingupon thisfacthe conjectured thatthe glycoria due. to piqure. may. stand. in an. intimate. relation to the function. of the. suprarenals. 2.

(3) Hamazaki‑On. Thereafter,. Blum's. extirpation. of. view. the. Adrenalinaemiaresultingfrom CentralGlycosuria,etc.. was. supported. by. Mayer's. to. method,. piqure. 249. experiments. made. after. the. suprarenals.. Experiments:‑ Piqure. was. occipitalis from. the. time. of. the. performed. according. puncture. operation.. was. made.. Tests. of. Eckhard's. The. examination. hyperglycemia. and. is, after. made. glycosuria. two, were. the. four,. made. removal. six,. of. eight,. twice,. the. membrana. twelve. i.e.,. and. before. atlanto‑. 24. hours. injection. and. after at. the. sacrifice.. The. results. are. shown. in. the. Table. following. Ⅰ.. L.. p. g.=Langhan's. The. Series. positive. hyperglycaemia. and. tables. Diabetic. Note: Hist.cell=Histiocytic cell.. Ⅰand. Ⅱ.. puncture.. Series. Ⅰ.. B. p.g.=Best'spositive granules .. granules.. glycosuria. in the. Tables. Ⅰ, Ⅱ, Ⅲ. and. Ⅵ. were. proved. by. Dr . Fujihara.. Ⅰ. The. 26. that. was. and. Best's. positive. 28, sparcely. granules. in Case. Blood‑sugar:. are contained. 27, none. it increases. in. from. in the. Cases. two. 29. and. to eight. histiocytic. cells of. the. milk‑spots. abundantly. in. Cases. 25. 30.. hours. after. the. puncture. and. returns. to the. normal. after. twelve. hours. Glycosuria:. positive. Liver‑glycogen: and. 24. Series. Case. hours,. 3. two. to. eight. in traces. two. hours. hours. after. the. after. the. puncture,. puncture, from. only four. trace. to eight. twelve hours. hours. after .. gradually. increases. , twelve. normal.. Ⅱ. In. Cases. 36. in trace. Blood‑sugar: hours.. nearly. from. 31. and and. 33. the. in Cases increases. Best's 32 from. positive. and two. 35. granules. can. be. found. numerously. , in Case. 34. in medium. quantity,. in. none. to. eight. hours. after. the. puncture. and. it returns. to. the. normal. after. 24. ,.

(4) 250. Hamazaki‑On Adrenalinaemia resulting from CentralGlycosuria, etc. Glycosuria: in allcasespositive. Liver‑glycogen:from two to eighthours afterthe punctureintrace,twelveand 24 hours somewhat abundant. Table. In. the. hyperglycemia. remarkably thus;. increases in. Series. Ⅱ.. Diabetic. produced as. compared. by.. puncture.. with. means the. of. normal.. Ⅰ, (++):(+):(±):(‑)=3:0:1:2,. Series. Ⅱ.. puncture. glycogen. The. above in. Series. of. results Ⅱ,. the may. milk‑spots be. shown. (++):(+):(±):. (‑)=2:1:1:2.. 2. Diuretin injection. In 1895 Jacoby observed that the injectionof the derivativesof caffeinin the rabbit previouslyfed with food richin carbohydrate causes a glycosuriaand he termed it "Ni. erendiabetes".Richter,however,opposed Jacoby'sview, recognizing the occurenceof hyperglycemiaand diminutionof liver‑glycogen in the case of diuretin‑glycosuria. He thoughtthatitisthe so‑called liver‑glycosuria due tothe increased reduction ofliver‑gly cogen. Later,Rose emphasizedfrom hisexperimentthathyperglycemiaappearsprior to glycosuria. Experiment:‑. 10 c.c.of 6% diuretin in normal salinesolution was subcutaneously injected per kilogramof body‑weight and examinedat intervals of one,two,three,four,fiveand seven hours. Hyperglycemiaand glycosuria were testedtwice;oncejustbeforeinjection and the othertimewhen sacriced. The. results are shown. in the following. table. 4.

(5) Hamazaki‑On Adrenalinaemia resulting fromCentralGlycosuria, etc.. Table. Ⅲ.. 251. Diuretin‑injection.. The Best'spositivegranulesof the milk‑spotsare presentabundantly in Case 22, moderately in Cases 20, 21 and 23, in tracein Case 19 and none in Case 24. Blood‑sugar: itreachesthe maximum threehours afterthe injection and graduallydecreasesuntilit nearzly returnsto normal afterseven hours. Glycosuria: from two to fivehours afterthe injection, positive, sevenhours after,in trace. Liver‑glycogen:only in tracein Case 20, somewhat highly diminishes in Cases 19, 22 and 23 and mode‑ ratelyin Cases 21 and 24. Judging. from. the. diuretin‑injection may. be. above. the. shown. experiment,. glycogen. in this. way;. in. 1901. rabbit. and. et. proved. al.. Zulzer,. Blum. in. that. Metzger. et. al.. injected. causing. the. milk‑spots. Peripheral. subcutaneously. succeeded. the. be. stated. that. decidedly. in. hyperglycemia. increases.. The. suprarenals. in. due. to. proportion. (++):(+):(±):(‑)=1:3:1:1.. Ⅲ.. In. it may. an. effective rocognized. glycosuria.. the. experimental substance. extract. of. the. glycosuria. of. that. glycosuria. was. subcutaneously. the is. Later,. suprarenal preceded. Herter. glands. is. by. hyperglycemia.. the. ratio. the and. the. dog. and. Richards adrenalin.. Experiment:‑ Series. Ⅰ. 0.4 c.c. of adrenalin. weight twice,. 5. and. examined. i.e., just. before. chloride. after. one,. injection. 1:1000 two,. three,. and. at the. four, time. five and. seven. of sacrifice.. injected hours.. with. Hyperglycemia. of and. per. kilogram. glycosuria. of were. body‑ tested.

(6) 252. Hamazaki‑On Adrenalinaemia resulting from CentralGlycosuria, etc.. The. following. table shows Table. the results. Ⅳ.. Adrenalin‑injection.. Series. Ⅰ.. The Best'spositivegranulescan be detectedsparcelyin Cases44 and 48, none in Cases 43, 45, 46 and 47. Blood‑sugar: itincreasesremarkably one hour afterthe injection and reachesthe maximum afterfourhours. After seven hours,stillthe hyperglycemiaremains inmedium degree. Glycosuria: in allcases,positive. Liver‑glycogen:in Cases 43, 47 and 48 diminishesmoderately,in Cases 44 and 45 more decreasesand in Case 46 stillmore. Series. Ⅱ. Table. Ⅴ.. Adrenalin‑injection.. Series. Ⅱ.. 6.

(7) Hamazaki‑On. The. quantity. In. of the. all cases. no. seven. injected. was. incraesed. positive. granules. are. present.. one. hour. increases. hours,. markedly. hyperglycemia. Glycosuria:. From. above. adrenalin‑injection. 52,. 53. and. 54. kilogram. injection. reaches. one in. the. diminishes. may. in. Series. and. observe. the. milk‑spots. in Cases. that. very. in. much. (++):(+):(±):(‑)=0:0:2:4,. =0:0:0:6,. and. of body‑weight.. maximum. three. hours. after.. degree.. remarkably. experiment glycogen. namely;. the. in medium. to 0 .8 c.c. per. 253. positive.. in Cases. the. after. still remains. in all cases. Liver‑glycogen:. es,. adrenalin. Best's. Blood‑sugar: After. Adrenalinaemiaresultingfrom CentralGlycosuria,etc.. in. 49,. the. 50. 51. still more.. hyperglycemia. decreases. Series. and. or. I and. due. totally. to. disappear. (++):(+):(±):(‑). Ⅱ. Ⅳ.. Renal. glycosuria.. v. Mering reportedthat in phloridzin‑administration in the dog glycosuriaappeared remarkably, but hyperglycemia did not. He claimed thisto be a case of renal glyco suria. However, Pavy, Biedl and Kolisch recognized hyperglycemia in phloridzin‑gly cosuria and opposed Mering's view. In phloridzin‑glycosuria, Minkowski found that the. sugar‑contents. of. diminished. with. the. Later,. Mering's. blood. is. Erlandsen,. not. augmented,. Czyhalz. but. and. on. the. Schlesinger,. contrary,. it. Lewandowsky. is. frequently. et. al.. agreed. view.. Experiment:‑ 2%. phloridzin‑suspension. the. body‑weight. were. tested. and twice,. The. and. Best's. none. from. are. positive. in Cases. Blood‑sugar: and. 0.102. influence these. on cases,. (+):(±):(‑)=1:1:1:2.. 7. on. the. in are. by an. a mere above. was two,. subcutaneously three,. and. Table. four,. at the. injected five. time. and. with. seven. the. dose. hours.. of. 10 c.c.. per. Hyperglycemia. kilogram. and. of. glycosuria. of sacrifice.. Ⅵ. found. numerously. in Case. 41,. moderately. in. Case. 42,. sparcely. in. before. the. Cases. 38,. 39.. in all cases. the. one,. injection. shown. changes. 0.093,. Liver‑glycogen: Form. before. granules. 37, no. to. Glycosuria:. after. i.e., just. results. The 40. in olive‑oil. examined. time.. average. It ranges 0.095. from. just. before. 0.104. to. 0.089,. on. an. average. 0.097,. injection. sacrificed.. positive. trace. results renal‑glycosuria. (++):(+):(±):(‑)=1:1:2:2,. in Cases one. 39, can. 41 see. caused. and. in. that. the. other. cases,. nearly. glycogen‑contents by. normal. of. the. phloridzin‑injection. is. nearly. milk‑spots The. similar. to. the. has. proportion normal,. no in. (++):.

(8) 254. Hamazaki‑On Adrenalinaemia resulting from CentralGlycosuria, etc.. Table. Ⅴ.. Ⅵ.. Phlaridzin‑injection.. Diuretin‑injection. followed. by. adrenalin.. Glycogen in the milk‑spotsevidentlyincreasesat centralhyperglycemia, while it highlydiminishes or totally disappeares at peripheral hyperglycemia. So itisofinterest to see ofthe increased glycogenin the milk‑spots as the results of diuretin‑injection isin some way influenced by adrenalin. Experiment:‑ 6% diuretinin normal salinesolutionwas subcutaneouslyinjectedin such proportionas 10 c.c.per kilogram of body‑weight and fourhours laterthe animal was sacrificed.1/4, 1/2, 1 and 2 hours before the time of sacrifice adrenalin and. chloride. glycosuria The The. Cases. 67,. results. are. positive. 68. 69,. and. Liver‑glycogen: in. subcutaneously. determined. Best's. Glycosuria:. only. was. were. shown. while. in. in Case. Case. before in. granules. in all cases. injected. just. Table. of the 70. in. diuretin,. of. 0.4 c.c.. adrenalin‑injection. per. kilogram. of. and. at the. a trace. in. body‑weight.. time. of. Blood‑sugar. sacrifice.. Ⅶ.. milk‑spots. (control). a dose. the. can. be. detected. granules. exist. in. Case. 66,. without. even. a. trace. in. abundantly.. positive. 66. slightly. decreases,. in. Cases. 67,. 68. and. 70. strongly. decreases. and. in. Case. 69. a trace.. From the above results,itmay be concluded that the increasedglycogen‑depot of the milk‑spotsin the case of diuretin‑injection isalmost entirelydiminishesby adrenalin‑ injection. 8.

(9) Hamazaki‑On Adrenalinaemia resulting from CentralGlycosuria, etc.. Table. Ⅵ.. Ⅶ.. Review. Diuretin‑injection. of. literature. followed. and. by. 255. adrenalin.. discussion.. As Blum succeededinproducingan experimental glycosuria by the injection of the extractofthe suprarenals he inferred thatthe glangsmay be able to cause glycosria alsoinvivoand thattheremay be an intimaterelation between diabeticpunctureand the suprarenals.His inference was laterverified by Andre Mayer'sexperimentthatthe glycosuria causedby puncturedoes not occur afterboth the adrenalswere extirpaed. Later,Kahn and Starkenstein, Landau et al. affirmedthe resultsof thisexperiment. The diuretin‑hyperglycemia isalsoinhibited by the extirpation of both the adrenalsor splanchnicotomyas formed out by Nishi. Many other cases of centralhyperglycmia, suchas producedby morphin‑,strychinin‑, chloroform‑administration, stimulation of the sensorynervesetc.are alsochecked by the cuttingof the splanchnici that are the se cretorynervesofthe adrenals(Pollak). These experimentsstronglysupportedthe adrenalin‑theory heldby Blum et al.,but atthe same timeone shouldnot neglectto seehow the liver‑glycogen is affected by the removalofthe adrenals.Schwarz,Kahn and Starkenstein, Porgesetal.noticedthatthe liver‑glycogen isstronglydecreasedor totally disappearedafterthe extirpation of both 9.

(10) 256. Hamazaki‑On Adrenalinaemia resulting from CentralGlycosuria, etc.. the adrenals,whereas the factthatpiquremade afterthe removal of the adrenalswas not successful may be due to the lack of liver‑glycogen. Furthermore,in the case of the removal ofadrenalsthe quantityof urineis usuallymarkedly diminished.In such case,as iswellknown, glycosuria oftendoes not takeplace,inspiteofthe presenceofa remarkablehyperglycemia.Therefore,inthe experimentof Mayer et al.,itiscoside‑ rablethat hyperglycemiaexisted.Wertheimer and Battezstatedthat hyperglyceia due to piqurecan be demonstratedin the catfrom which the adrenalshave been extir patedand Mc Guigan alsosucceededin producingthe salt‑glycosuria afterthe removal of theadrenals. Accordingto Kahn and Starkenstein, the liver‑glycogen of the rabbit contraryto otheranimalsisnot influenced by the removal of the adrenals,and yet no glycosuria appear by the puncture. Freund and Marchand proved,however,that the hyperglycemianot accompaniedby glycosuria almostalwaysappearsby the puncturein the epinephrotomizedrabbits.Reducing from thisfact,they insisted that the effct causedby the piquredoesnot affect the adrenalsatall,but the liverdirectly. Itisto be noted thatthereare many differences between the glycosuriadue to pi qure and adrenalin‑glycosuria. Piquredoes not work on any starvedanimals(Bernard, Dock et al.), while the adrenalin‑glycosuria appearsin such cases (Bang). And itis consideredby many thatinthe caseof piqurethe sugarismainlyderivedfrom the liver‑ glycogenwhileat the adrenalin‑glycosuria itcomes from both the liver‑andmuscle‑gly cogen. According to Pollak,by continuedadministration of adrenalinin the starved rabbit,the liver‑glycogen is formed and muscle‑glycogendeminished. Hirudin previ ouslyadministered intravenously inhibits the adrenalin‑glycosuria, but ithas no influnce on the diuretin‑glycosuria (Miculicich).On narcotizedanimalspiquredoes not work, whileadrenalin does (Eckhard). Chloralhydrat represseshyperglycemiadue to piqure, but itdoes not acton adrenalin‑glycemia (Bang). All what have been saidoppose adrenalin‑theory and we are quiteata lossto why thereexistsuch remarkabledifferences betweenthe actionof the mobilizedadrenalin y piqureand thatofinjected adrenalin.Although at firstCl. Bernard,Eckhard, Pfluger et al.consideredthat the actionof puncture reachesdirectlythe liver‑cells via e splanchnici, a new theorywas put forthbasingupon Mayer'sexperiment, thatthe stimu limay passthrough the adrenals.However, how the stimuliwhich have reached the adrenalsget to the liver‑cells isstill an open question. One naturallytakes recoursein adrenalinemia to explainthis,but ithas met with many objections as above‑mentined. Therefore,Nishiinferred thatthe adrenalsand the livermay be connectedwith some nerves. Lately,basingupon Lichtwitz's view thatadrenalin runsin the sympathic sys 10.

(11) Hamazaki‑On. Adrenalinaemiaresultingfrom CentralGlycosuria,etc.. 257. tem, Tammann puncturedthe rabbitafterunilateral extirpation of the adrenalsand cut the liverintotwo partsalongthe Lig. longitudinale hepatisand measured the quantity ofglycogencontainedin each halfseparately.He found out thatin the halfon the side withoutthe adrenal,glycogenisconsumed far lessthan in the oppositehalf. "Eine befriedigende Erklarung diesesPhenomen ist" he said "nur moglich unter der An nahme, dass das Adrenalinnichtin der Blutbahn,sondern im Sympathischen Nerven seinenWeg zur Leberzellen nimmt". Whether or not the adrenalin‑quantity of the blood increasesby piqurehas been much discussed by many investigators. Using Ehrmann's frog‑eye‑reaction Waterman and Smit reportedthatthe adrenalin‑quantity of the blood is hightenedby punture. But Borberg and Lopez deniedthis. Kahn also proved that neitherthe serum of the arterial blood nor thatof the venous blood showed any vasoconstrictive actionin the caseof puncture. Trendelenburgand Fleischhauermeasured the quantityof adrealin which isnecessaryto produceglycosuria by continuedintravenousinfusionof adenalin and they confirmedthatthisamount is sufficient to hightenthe blood‑pressure.But sinceneitherpiqurenor diuretin‑injection has any influence whateveron the bood‑pres sure,theydeclinedto acceptthe theorythatcentralglycosuriais attributable to are‑ nalinemia.Nishireportedthatafterdiuretin‑injection the blood‑serumshows no effet at allon the frog'seye. By means of unilateral removal of the uppermost cervicl ganglion,Negerinsucceededin givingthe irisan adrenalin‑sensibility on the oerated side. From the animal thus operated,he obtainedresultsthat epinephrindischared from the adrenalsafterpuncturereachesthe eye in sufficient amount to produce glyco suria. K. Shimidzualsorecognized the appearanceof adrenalinemiain similarexperi ments. However, Elliotprovedthatin the similarexperimentsmydriasisstillappears in the animal withoutthe adrenals.Carrasco‑Formiguera puncturedthe cat with the denervatedheartfollowing Cannon'sadrenalin‑test and made out thattachycardiaoccu red immediatelyafterthe piqure. This phenomenon was inhibited by the extirpation of the adrenalsor theirdenervation.When the abdominal cava was temporarilyclamped above the lumbo‑adrenalvein,the heart ratewent down, and when the clamp was removed,itquicklyincreased.He concludedfrom thesefactsthat"the piqureproduces the dischargefrom the adrenalglandsof a productthat reachesthe arterial blood in sufficient amount to exert on otherorgans an actionidentical to that of adrenalin". Trendelenburgand Fleischhauer emphasizedthatthe glycosuria due to piqureisnot the actionof the hormon dischargedfrom the adrenals,but the formerlatelychanged his view by new experimentthat immediatelyafterpiqurethe adrenalinemiatakesplace 11.

(12) 258. Hamazaki‑On Adrenalinaemia resulting from CentralGlycosuria, etc.. which is capableof elicitting the glycosuria.With no experimentalbasishe made a compromise thatthe glycogen‑mobilization by punctureinducesadrenalinemia, and in additionthe impulsesmay reachthe liver‑cell by way ofthe nerves. Many investigators judged indirectly the quantityofadrenalininthe blood from the amount ofchromaffinsubstanceofthe adrenalsinthecaseofcentral glycosuria.Jaisch, Kahn, Pfeiffer et al.reportedthatthe chromaffin substancehas diminishedby puncture. Negerin and Brucke, and Fujiiadmittedthe above mentionedfact,yet at the same time they noticedthatthe similar phenomenon can be observedeven when puncturefailsto producethe glycosuria.Fujiiobtainedthe resultthat when unilateral splanhnicotomy was done priorto the piqure,the adrenalon the denervatedsidedid not any change at all,while the chromaffinsubstanceof the opposite. gland was markedly diminishedor sometimesentirely disappeared. Itshouldbe stated,however, thatthe decreaseof the chromaffinsubstanceof the adrenalsmay takeplaceby the increaseddischargeof adrenalin, and by the depressed adrenalin‑formation justas well and thismakes the correlation between the amount of chromaffinsubstanceand thatof adrenalinin blood very uncertain.Furthermore,this uncertainty isenhanced by the factthatbrown coloringof the adrenalsby bichromate‑ fixation can not be taken as a reliable adrenalin‑reaction (Kahn, Trendelenburg, Kut schera‑Aichberger etal.). I have made some observations on the changes of chromaffinsubstanceof the adrenalssubsequentto diuretin‑and adrenalin‑injection. In each case the rightadrenal was made transparentmacroscopic preparationaccordingto Negerin and Brucke's method and the leftone intoserialparaffin sections.In the case of diuretin‑injion the chromaffin substancesometimesdiminishedas compared with the normal one. So alsowiththe caseof adrenalin‑injection. No fundamentaldifferences between diretin‑ and adrenalin‑injection could be made out regardingthe amount ofchromaffinsubstnce. From what has been saiditwillbe seen thatthereis still a divergenceof views concerningadrenalinemia by the centralglycosuria.My experimentsyieldedthe reslts as beforementioned thatthe glycogenin the milk‑spotsmarkedly increasesby piqure and diuretin‑injection, whileitverymuch decreasesor entirely disappearsby adrenlin‑ injection, and thatthe increasedglycogen‑depotof the milk‑spotsby diuretin‑injon isevidently reducedby adrenalin‑injection. These factsare naturallyincompatiblewith the adrenalinemia‑theory.. 12.

(13) Hamazaki‑On. Adrenalinaemiaresultingfrom CentralGlycosuria,etc.. Ⅶ.. 259. Conclusions.. 1. The quantityof glycogenin the milk‑spots remarkablyincreases at cenal glycosuria, whileitmarkedlydecreases or entirely disappears at peripheral glycosia. Itisnotinfluenced atallatrenalglycuria. 2. Glycogen‑depot of the milk‑spots increased by diuretin‑injection is maredly diminished by adrenalin‑injectn. 3. Takingthesefacts intoconsideration, itwillbe saidthatthe originof cetral glycosuria. may. not be the same. as that of adrenalin‑glycosuria.. In ending,I wish to acknowledge my indebtednessto Profs.O. Tamura and H. Tanabe and also to expess my gratitudeto Dr. Fujiharawho has kindlyput valuablematerialsat my disosal.. Bibliography.. Bang, DerBlutzucker. Wiesbaden. 1913. Bernard,Cl., cited inPfluger.Biedlu.Kolisch, UeberPhlorizin‑Diabetes. Verhandl. d.Kong.f.inn.Med.Bd.18,S.573,1900. Blum, cited inBang. Borberg & Lopez, ditto. Cannon, The Isolated Heartasan Indicator ofAdrenalin Secretion by Paln, Asphyxia andExcitement. Americ. Journ. ofPhysiol. Vol.50,p.399,1919/20. Cannon & Raport, Further Observation ontheDenervated HeartinRelation toAdrenalin Secretion. Ibid. Vol.58,p.308,122. Carrasco‑Formiguera, The Production of AdrenalDischarge by Piqure. Ibid.Vol.61,p. 254, 22. Czyhalz & Schlesinger, citedinSato. Dock, UeberdieGlykogenbildung inderLeberund ire Beziehungen zum Diabetes. Pfluger's Arch.Bd.5,S.571,1872. Eckhard, cited inPfluger.Ehrmann, Uebereinephysiologische Wertbestimung desAdrenalins undseinen Nachweisim Blut.Arch.f.exper.Path.u. Pharm.Bd.53,S.97,1905. Elliott,cited inCarrasco‑Formiguera.Erlandsen, Experintelle Untersuchungen uberdenPhloridin‑Diabetes. Bioch. Ztschr. Bd.23,S.329,1910. Freund u. Marchand, UeberdieWirkungendesZuckerstiches nachNebennierenexstirpation. Arch.f.exper. Path.u.Pharm.Bd. 76,S. 324,1914. Fujii, Tohoku‑Igakkai‑Zasshi. Bd.3,S. 67,1918. Hamazaki, Y., The Milk‑sos, "Tacheslaiteuses" in Omentum. Histological and Biological Studies made Especially on TheirMesothial Cell‑layer andHistiocytes. Okayama‑Igakkai‑Zasshi. No.429/30, p.1,1925. Hamazaki, Y., Studies on GlycogenintheMilk‑spots oftheOmentum.Ibid.No.433,p.1,1926. Herter & Richards, citedin Bang. Jacoby, Ueberkunstlischen Nierendiabetes. Arch.f.exper. Path.u.Pharm.Bd.35,S.213,1895. Jarisch, UeberdenMechanismus derPiqure‑Glykosurie. Pfluger's Arch.Bd.158,S.478,1914. Kahn u. Starkenstein, UeberdasVerhalten desGlykogens nachNebennierenexstirpation. Pfluger's Arch.Bd. 139,S. 181,1911. Kahn, ZurFragenachderAdrenalinaemie nachdem Zuckerstich. Pfluger's Arch.Bd.144,S. 251,1912. Kahn, Weitere Untersuchungen zurAdrenalinaemie. Ibid. S.396. Kahn, Ueberdienach zentral ReizungzurStorung desKohlenhydratstoffwechsels. Ibid.Bd.169,S. 326,1917. Kutschera‑ Aichberg, citedinTrendelenburg.Lewandowsky, citedinSato. Lichtwitz, UeberWande rungdesAdrenalins im Nerven. Arch.f.exper. Path.u.Pharm.Bd.58,S.221,1908. Lichtwitz, Ueber den MechnismusderNebennieren‑bezw. Adrenalinwirkung. Ibid.Bd.65,S.214,1911. Lichtwitz, Das Nebennierenproblem. Kl.Wchnschr. Jahrg. 1,S.2245,1922. Mayer, Andre, cited inBang. Mc Guigan, cited inFreund& Marchand. v.Mering, UeberDiabetes mellitus, I.Ztschr. f.kl.Med.Bd. 14,S.405,1888. v.Mering, UeberDiabetes mellitus, II.Ibid. Bd.16,S.431,1889. Metzger, citedinBang. Miculicich, UeberGlykosuriehemmung. Arch.f.exper. Path.u.Pharm.Bd.69,S.128, 13.

(14) 260. Hamazaki‑On. Adrenalinaemia. resulting. from. Central. Glycosuria,. etc.. 133,1912. Negerin, citedinCarrasco‑Formiguera. Negerin & Brucke, citedinFujii. Nishi, UeberdenMechnismus desDiuretinglykosurie. Arch.f.exper. Path.u.Pharm.Bd.61,S.401,1909. Pavy, citedin Bang. Pfeiffer,UeberVeranderungen des Nebennierenorganes nach nervosen und toxisen Schadigungen. Ztschr. f.d.ges.exper. Med.Bd.10,S.1,1919. Pfluger, Glykogen. Pfluger's Arch.Bd.96, S.1,1903. Pollak, UeberGlykogenbildung beiKarenzkaninchen unterdem Einfluss vonAdrenalic. Arch. f.exper. Path.u.Pharm.Bd.61,S.166,1909. Pollak, Kritisches und Experimentelles zurKlassifcion derGlykosurie. Ibid. S.376. Porges, cited inBang. Rose, Der Blutzuckergehalt desKannincns, seineErhohungdurchden Aderlass, durch dieEroffnungder Bauchhohleund durch die Nierenausschaltung und seinVerhaltenim Diuretindiabetes. Arch.f.exper.Path.u.Pharm. Bd. 50,S. 15,1903. Richter, Diuretin und Glykosurie. Ztschr.f.kl.Med. Bd. 35,S.463,1898. Sato, Kozo, Tohoku‑Igakkai‑Zasshi. Bd. 6,S.70, 77,111,1922. Schwarz, Ueber einige Ausfallserscheinungen nach Exstirpation beiderNebennieren. Wien. kl.Wchnschr.Nr. 51,S.1783,1909. Shimidzu, K., Quantitative MessungenderAdrenalinsekretion nach dem Zuckerstich und nach Vergiftungen. Arch.f.exper.Path u.Pham. Bd. 103,S.52,1924. Tammann, Der Glykogengehalt der Leber nach einseitiger Nebennierenexstirpation. Ztschr.f.d. ges.exper.Med. Bd. 40, S. 361,1924. Trendelenburg u. Fleischhauer, Ueber den Einfluss des Zuckerstiches aufdieAdrenalin‑ sekretion der Nebennieren. Ibid.Bd. 1,S. 369,1913. Trendelenburg, Ueber den AnteilderAdrenalin‑ sekretionan der Zuckerstichwirkung Pfluger's Arch. Bd. 201, S. 39, 1923. Waterman u. Smit, Nebenniereund Sympathicus. Ibid.Bd. 124,S.198,1908. Wertheimer & Battez, citedinFreund& Marchand. Zulzer, citedinBang.. 内. 容. 大. 意. 乳斑糖原質ノ消長ヨリ見タル中樞性糖尿ニ於ケル「アドレナリン」血症ノ疑義岡山醫科大學病理學敎室(主任,田村敎授). 濱崎幸雄中樞性糖尿ノMechanismusニ hard,. Pfluger氏. 論ジ, Blum,. 關シテハ大別スレバ三樣ノ説アリ.. Bernard,. Eck‑. 等ハ糠中樞ヨリノ興奮ハ交感神經系ヲ經テ肝ニ直達スルモノナリト. Mayer氏. 等ハ交感神經ヲ經テ副腎ニ達シ其ノ分泌亢進ヲ惹起シ「アド. レナリン」血症ヲ招來スルニ因ルモノナリトナシ, Nishi, Tammann氏ハ神經ヲ以テ連絡スルモノナラント推論シ, Kahn,. Trendelenburg氏. 等ハ副腎ト肝等ハ肝直達説ト. 副腎介在説トノ折衷説ヲ唱ヘ,各自々説ヲ高唱シテ互ニ讓ラズ. 著者ハ諸種ノ實驗的糖尿病ニ於ケル乳斑糖原質ノ消長ヲ檢セシ所,ソ. ノ糖原量ハ. 中樞性糖尿症ニ於テハ著シク增加シ,末梢性糖尿症ニ於テハ之ニ反シラ甚シキ消耗乃至消失ヲ來ス.又. 「ヂクレチン」注射ニヨリテ增量セル糖原質ハ「アドレナリン」. 注射ニヨリテ著シク減少スルヲ確認セリ. 本實驗成績ハ中樞性糖尿ノ本態ヲ「アドレナリン」血症ニ歸セントスル説トハ相容レザル所,從. ツテ中樞性糖尿症ト末梢性糖尿症トハソノ本態ニ於テ同一ノモノト見. 做スヲ得ズ. 14.

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岡 山 醫 學 會 雜 誌 第 四百 三 十 四 號 大 正 十 五 年 三 月 三 十 一 日發 行

岡山醫學會雜誌第四百三十四號大正十五年三月三十一日發行