㧙 㧙 9.⣲≌․⇣⊛ࡋ࡞ࡄT ⚦⢩ߩ⣲≌ౝᶐẢᯏ᭴ߦ߅ߌࠆ ⴊ▤ౝ⊹⚦⢩ߩᓎഀߩ⸃ ዊ᧻ᐢޔቝ㜞ᕺሶ 㜞⍮ᄢቇ∉ቇᢎቶ ޣ⎇ⓥ⢛᥊ߣ⋡⊛ޤ ㄭᐕޔ࿕ᒻ⣲≌ߩᜎ⛘ߦߪ⚦⢩்ኂᕈ T ⚦⢩(CTL)ߛߌߢߥߊޔ⣲≌․⇣⊛ࡋ࡞ࡄT ⚦⢩(Th)ߩ⣲ ≌ౝᶐẢ߇㊀ⷐߢࠆߣ␜ໂߐࠇߡࠆޕߎࠇ߹ߢߦᚒޘߪޔ⣲≌․⇣⊛ Th ߩ⣲≌ౝᶐẢߦߪޔⴊ▤ ౝ⊹⚦⢩(EC)ߣ᮸⁁⚦⢩(DC)ߩਔᣇߦ MHC class II ಽሶߩ⊒߇ᔅⷐߢࠆߎߣࠍࠄ߆ߦߒߚޕߒ ߆ߒߥ߇ࠄޔታ㓙ߦ EC ߇⣲≌ᢿ ࠍขࠅㄟߺޔMHC class II ಽሶߦ⣲≌᛫ේࠍឭ␜ߔࠆߩ߆ᧂߛࠊ ߆ߞߡߥޕߘߎߢᧄ⎇ⓥߢߪޔEC ߇⣲≌ᢿ ࠍขࠅㄟߺޔ⣲≌᛫ේࠍឭ␜ߔࠆᯏ⢻ࠍᨐߚߔ߆ߦ ὶὐࠍ⟎ߡ⎇ⓥࠍㅴߚޕ ޣᣇᴺޤ
ࠕࡐ࠻ࠪࠬࠍߎߐߖߚ E.G7-OVA(OVA transfected EL4)ࠍⰯశᮡ⼂ߒޔHUVEC ߹ߚߪ F-2(CBF1 ࡑ࠙ࠬ↱᧪ߩⴊ▤ౝ⊹⚦⢩⣲≌ᩣ)ߦ I-Adࠍ transduce ߒߚ F-2I-Adߦขࠅㄟ߹ߖޔὶὐࠩ㗼 ᓸ㏜ߢⷰኤߒߚޕߘߩ㓙ޔE.G7-OVA ߦ Integrin-binding motif ߢࠆ RGD(Arg-Gly-Asp)ࡍࡊ࠴࠼ࠍઃട ߒޔࠝࡊ࠰࠾ࡦൻߒߚޕ
߹ߚޔ⣲≌᛫ේࠍขࠅㄟ߹ߖߚ F-2I-Adߦࠃࠅޔߤߩ⒟ᐲ T ⚦⢩߇ᵴᕈൻߐࠇࠆ߆ޔCD69 ߩ⊒ ⺃ዉ߅ࠃ߮ Boyden chamber ࠍ↪ߚ migration assay ߦࠃࠅ⺞ߴߚޕ
ޣ⚿ᨐޤ HUVECޔF-2I-Adߦ⣲≌⚦⢩ࠍขࠅㄟߎߣࠍ⏕ߒޔRGD ߢࠝࡊ࠰࠾ࡦൻߔࠆߎߣߦࠃࠅޔ ECߩขࠅㄟߺ⢻߇ะߔࠆߎߣ߇ࠄ߆ߣߥߞߚޕ ߹ߚޔ᛫ේỚᐲ߇ૐ⣲≌⚦⢩ࠍขࠅㄟࠎߛ EC ߦߪޔin vivo ߢ Th ߩ⣲≌ౝᶐẢࠍଦㅴߔࠆ⢻ജߪ ࠆ߇ޔin vitro ߢ CD69 ߩ⊒⺃ዉࠍߎߔ⢻ജ߇ߥߎߣ߇ࠊ߆ߞߚޕߘߎߢޔ᛫ේࡍࡊ࠴࠼Ớᐲ ࠍᄌ߃ߡᗵߒߚ EC ࠍ᛫ේឭ␜⚦⢩ߣߒߡ♽⛔⊛ߦ⺞ߴߚߣߎࠈޔCD69 ߩ⊒⺃ዉ߇ߎࠆߦߪච ಽߢߥᗵỚᐲߢ Th ߩ⒖േ߇ߎࠅޔCD69 ߩ⊒⺃ዉ߇ߎࠆỚᐲߢߪ Th ߇ EC ߣᒝߊળวߒ ߡޔߒࠈ⒖േ߇ૐਅߔࠆߎߣ߇ࠊ߆ߞߚޕ