サラブレット種雌馬の生殖周期各ステージにおける内分泌調節機構に関する研究

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Title サラブレット種雌馬の生殖周期各ステージにおける内分泌_{調節機構に関する研究( 本文(Fulltext) )} Author(s) 原田, 健弘 Report No.(Doctoral Degree) 博士(獣医学) 甲第453号 Issue Date 2016-03-14 Type 博士論文 Version ETD URL http://hdl.handle.net/20.500.12099/54526 ※この資料の著作権は、各資料の著者・学協会・出版社等に帰属します。

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ࢧࣛࣈࣞࢵࢻ✀㞤㤿ࡢ

⏕Ṫ࿘ᮇྛࢫࢸ࣮ࢪ࡟࠾ࡅࡿ

ෆศἪㄪ⠇ᶵᵓ࡟㛵ࡍࡿ◊✲

ᖺ

ᒱ㜧኱Ꮫ኱Ꮫ㝔㐃ྜ⋇་Ꮫ◊✲⛉

㸦

㸦ᮾி㎰ᕤ኱Ꮫ㸧

ᮾி㎰ᕤ኱Ꮫ㸧

ཎ⏣

ཎ⏣ ೺ᘯ

೺ᘯ

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┠ḟ ┠ḟ ┠ḟ ┠ḟ ❶❶❶ ⥴ㄽ❶ ⥴ㄽ⥴ㄽ⥴ㄽ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸯㸫㸯㸬㍍✀㤿⏕⏘ᆅ࡟࠾ࡅࡿ⌧≧ ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸯㸫㸰㸬㞤࣐࢘ࡢ⦾Ṫ≉ᛶ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸯㸫㸱㸬㞤࣐࢘ࡢዷፎᮇ࡟࠾ࡅࡿ⏕Ṫ⏕⌮Ꮫⓗ≉ᛶ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸯㸫㸲㸬ศፔᚋ᤼༸࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸯㸫㸳㸬◊✲┠ⓗ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸰❶ 㸰❶ 㸰❶ 㸰❶ ᮦᩱ࠾ࡼࡧ᪉ἲᮦᩱ࠾ࡼࡧ᪉ἲᮦᩱ࠾ࡼࡧ᪉ἲᮦᩱ࠾ࡼࡧ᪉ἲ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸰㸫㸯㸬౪ヨື≀࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖㸦5,$㸧࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸰㸫㸱㸫㸯㸬࢖ࣥࣄࣅࣥࡢ 5,$ 㸰㸫㸱㸫㸰㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦/+㸧ཬࡧ༸⬊่⃭࣍ࣝࣔࣥ㸦)6+㸧ࡢ 5,$ 㸰㸫㸱㸫㸱㸬ࣉࣟࣛࢡࢳࣥࡢ 5,$ 㸰㸫㸱㸫㸲㸬ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢ 5,$ 㸰㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥࡢ᫬㛫ศゎ⺯ගච␿ ᐃἲ㸦75),$㸧࣭࣭࣭࣭࣭࣭ 㸱❶ 㸱❶ 㸱❶ 㸱❶ ዷፎᮇ࡟࠾ࡅࡿ⏕ṪෆศἪᏛⓗኚ໬࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ዷፎᮇ࡟࠾ࡅࡿ⏕ṪෆศἪᏛⓗኚ໬࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ዷፎᮇ࡟࠾ࡅࡿ⏕ṪෆศἪᏛⓗኚ໬࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ዷፎᮇ࡟࠾ࡅࡿ⏕ṪෆศἪᏛⓗኚ໬࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸱㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸱㸫㸰㸬ᮦᩱ࠾ࡼࡧ᪉ἲ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸱㸫㸰㸫㸯㸬౪ヨ㤿㸱㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮

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㸱㸫㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢ ᐃ 㸱㸫㸰㸫㸲㸬⤫ィฎ⌮ 㸱㸫㸱㸬⤖ᯝ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸱㸫㸱㸫㸯㸬ࣉࣟࣛࢡࢳࣥ 㸱㸫㸱㸫㸰㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦/+㸧㸱㸫㸱㸫㸱㸬༸⬊่⃭࣍ࣝࣔࣥ㸦)6+㸧㸱㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ 㸱㸫㸱㸫㸳㸬ࢸࢫࢺࢫࢸࣟࣥ 㸱㸫㸱㸫㸴㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ 㸱㸫㸱㸫㸵㸬࢖ࣥࣄࣅࣥ 㸱㸫㸱㸫㸶㸬ዷፎ࣐࢘ࡢ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘ࡜᪥↷᫬㛫ࡢ㛵ಀ 㸱㸫㸱㸫㸷㸬ዷፎ࣐࢘ࡢ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘ࡜⫾Ꮚࡢᛶูࡢ㛵ಀ 㸱㸫㸲㸬⪃ᐹ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸲㸲㸲㸲❶❶❶❶ ศፔᚋⓎ᝟࣭᤼༸ࡢ⏕ṪෆศἪᏛⓗኚ໬࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ศፔᚋⓎ᝟࣭᤼༸ࡢ⏕ṪෆศἪᏛⓗኚ໬࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ศፔᚋⓎ᝟࣭᤼༸ࡢ⏕ṪෆศἪᏛⓗኚ໬࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ศፔᚋⓎ᝟࣭᤼༸ࡢ⏕ṪෆศἪᏛⓗኚ໬࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸲㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸲㸫㸰㸬ᮦᩱ࠾ࡼࡧ᪉ἲ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸲㸫㸰㸫㸯㸬౪ヨ㤿㸲㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ 㸲㸫㸰㸫㸱㸬㉸㡢Ἴ⏬ീデ᩿࡟ࡼࡿศፔᚋࡢ༸ᕢࡢほᐹ 㸲㸫㸰㸫㸲㸬ྛ✀࣍ࣝࣔࣥࡢ ᐃ 㸲㸫㸰㸫㸲㸫㸯㸬ࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖ἲ 㸲㸫㸰㸫㸲㸫㸰㸬㓝⣲ච␿ ᐃἲ㸦(,$㸧㸲㸫㸰㸫㸳㸬⤫ィฎ⌮

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㸲㸫㸱㸬⤖ᯝ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸲㸫㸱㸫㸯㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦/+㸧㸲㸫㸱㸫㸰㸬༸⬊่⃭࣍ࣝࣔࣥ㸦)6+㸧㸲㸫㸱㸫㸱㸬ࣉࣟࣛࢡࢳࣥ 㸲㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ 㸲㸫㸱㸫㸳㸬ࢸࢫࢺࢫࢸࣟࣥ 㸲㸫㸱㸫㸴㸬࢖ࣥࣄࣅࣥ 㸲㸫㸱㸫㸵㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ 㸲㸫㸱㸫㸶㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜࢚ࢫࢺࣛࢪ࣮࢜ࣝࡢศἪࣃࢱ࣮ࣥࡢẚ㍑㸲㸫㸱㸫㸷㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜ࢸࢫࢺࢫࢸࣟࣥࡢศἪࣃࢱ࣮ࣥࡢẚ㍑㸲㸫㸲㸬⪃ᐹ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸳❶ 㸳❶ 㸳❶ 㸳❶ ᡂ⇍㞤ࢧࣛࣈࣞࢵࢻࡢୗᆶయ࣭༸ᕢ࣍ࣝࣔࣥศἪࡢᖺ࿘ᮇⓗኚ໬࣭࣭ᡂ⇍㞤ࢧࣛࣈࣞࢵࢻࡢୗᆶయ࣭༸ᕢ࣍ࣝࣔࣥศἪࡢᖺ࿘ᮇⓗኚ໬࣭࣭ᡂ⇍㞤ࢧࣛࣈࣞࢵࢻࡢୗᆶయ࣭༸ᕢ࣍ࣝࣔࣥศἪࡢᖺ࿘ᮇⓗኚ໬࣭࣭ᡂ⇍㞤ࢧࣛࣈࣞࢵࢻࡢୗᆶయ࣭༸ᕢ࣍ࣝࣔࣥศἪࡢᖺ࿘ᮇⓗኚ໬࣭࣭ 㸳㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸳㸫㸰㸬ᮦᩱ࠾ࡼࡧ᪉ἲ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸳㸫㸰㸫㸯㸬౪ヨື≀ 㸳㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ 㸳㸫㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢ ᐃ 㸳㸫㸱㸬⤖ᯝ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸳㸫㸱㸫㸯㸬᏶඲⏕Ṫ࿘ᮇ 㸳㸫㸱㸫㸯㸫㸯㸬ࣉࣟࣛࢡࢳࣥ 㸳㸫㸱㸫㸯㸫㸰㸬/+ 㸳㸫㸱㸫㸯㸫㸱㸬)6+ 㸳㸫㸱㸫㸯㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ

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㸳㸫㸱㸫㸯㸫㸳㸬ࢸࢫࢺࢫࢸࣟࣥ 㸳㸫㸱㸫㸯㸫㸴㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ 㸳㸫㸱㸫㸯㸫㸵㸬࢖ࣥࣄࣅࣥ 㸳㸫㸱㸫㸰㸬㠀ዷፎᮇ 㸳㸫㸱㸫㸰㸫㸯㸬ࣉࣟࣛࢡࢳࣥ 㸳㸫㸱㸫㸰㸫㸰㸬/+ 㸳㸫㸱㸫㸰㸫㸱㸬)6+ 㸳㸫㸱㸫㸰㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ 㸳㸫㸱㸫㸰㸫㸳㸬ࢸࢫࢺࢫࢸࣟࣥ 㸳㸫㸱㸫㸰㸫㸴㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ 㸳㸫㸱㸫㸰㸫㸵㸬࢖ࣥࣄࣅࣥ 㸳㸫㸱㸫㸱㸬Ἢஙᮇ 㸳㸫㸱㸫㸱㸫㸯㸬ࣉࣟࣛࢡࢳࣥ 㸳㸫㸱㸫㸱㸫㸰㸬/+ 㸳㸫㸱㸫㸱㸫㸱㸬)6+ 㸳㸫㸱㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ 㸳㸫㸱㸫㸱㸫㸳㸬ࢸࢫࢺࢫࢸࣟࣥ 㸳㸫㸱㸫㸱㸫㸴㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ 㸳㸫㸱㸫㸱㸫㸵㸬࢖ࣥࣄࣅࣥ 㸳㸫㸱㸫㸲㸬ὶ⏘ᚋࡢ࣍ࣝࣔࣥศἪኚ໬ 㸳㸫㸱㸫㸳㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜ࢸࢫࢺࢫࢸࣟࣥࡢศἪࣃࢱ࣮ࣥࡢẚ㍑㸳㸫㸱㸫㸴㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜࢚ࢫࢺࣛࢪ࣮࢜ࣝࡢศἪࣃࢱ࣮ࣥࡢẚ㍑㸳㸫㸱㸫㸵㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜࢖ࣥࣄࣅࣥࡢศἪࣃࢱ࣮ࣥࡢẚ㍑㸳㸫㸱㸫㸶㸬࢖ࣥࣄࣅࣥ࡜࢚ࢫࢺࣛࢪ࣮࢜ࣝࡢศἪࣃࢱ࣮ࣥࡢẚ㍑

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㸳㸫㸲㸬⪃ᐹ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸳㸫㸲㸫㸯㸬㞤ࢧࣛࣈࣞࢵࢻࡢ⏕Ṫᶵ⬟ࡢᖺ࿘ᮇ 㸳㸫㸲㸫㸰㸬Ἢஙᮇ࡟࠾ࡅࡿྛ✀࣍ࣝࣔࣥศἪ 㸳㸫㸲㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢศἪࣃࢱ࣮ࣥࡢẚ㍑㸴㸴㸴㸴❶❶❶❶ ග⥺ฎ⌮ἲࢆᐇ᪋ࡋࡓᙜṓ㞤㤿ࡢ⏕ṪෆศἪᏛⓗኚ໬ග⥺ฎ⌮ἲࢆᐇ᪋ࡋࡓᙜṓ㞤㤿ࡢ⏕ṪෆศἪᏛⓗኚ໬ග⥺ฎ⌮ἲࢆᐇ᪋ࡋࡓᙜṓ㞤㤿ࡢ⏕ṪෆศἪᏛⓗኚ໬ග⥺ฎ⌮ἲࢆᐇ᪋ࡋࡓᙜṓ㞤㤿ࡢ⏕ṪෆศἪᏛⓗኚ໬࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸴㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸴㸫㸰㸬ᮦᩱ࠾ࡼࡧ᪉ἲ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸴㸫㸰㸫㸯㸬౪ヨື≀ 㸴㸫㸰㸫㸰㸬ග⥺ฎ⌮ἲ࡜ᐇ㦂᧯స 㸴㸫㸰㸫㸱㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ 㸴㸫㸰㸫㸴㸬ẟⰏࡢホ౯ 㸴㸫㸰㸫㸵㸬㞤࣐࢘ࡢึᅇ᤼༸ 㸴㸫㸰㸫㸶㸬⤫ィฎ⌮ 㸴㸫㸱㸬⤖ᯝ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸴㸫㸱㸫㸯㸬⾑୰࣍ࣝࣔࣥ⃰ᗘኚ໬ 㸴㸫㸱㸫㸯㸫㸯㸬ࣉࣟࣛࢡࢳࣥ 㸴㸫㸱㸫㸯㸫㸰㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦/+㸧㸴㸫㸱㸫㸯㸫㸱㸬༸⬊่⃭࣍ࣝࣔࣥ㸦)6+㸧㸴㸫㸱㸫㸯㸫㸲㸬࢖ࣥࣄࣅࣥ 㸴㸫㸱㸫㸯㸫㸳㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ 㸴㸫㸱㸫㸯㸫㸴㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ 㸴㸫㸱㸫㸰㸬ẟⰏࡢホ౯ 㸴㸫㸱㸫㸱㸬ึᅇ᤼༸᫬ᮇ

(8)

㸴㸫㸲㸬⪃ᐹ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸵㸵㸵㸵❶❶❶❶ ⥲ᣓ࣭࣭⥲ᣓ࣭࣭⥲ᣓ࣭࣭⥲ᣓ࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ 㸵㸫㸯㸬ࢧࣛࣈࣞࢵࢻ✀࡟࠾ࡅࡿዷፎᮇࡢෆศἪᏛⓗኚ໬ 㸵㸫㸰㸬ᡂ⇍㞤ࢧࣛࣈࣞࢵࢻࡢୗᆶయ࣭༸ᕢ࣍ࣝࣔࣥศἪࡢᖺ࿘ᮇⓗኚ໬ 㸵㸫㸱㸬ศፔᚋึᅇⓎ᝟ࡢෆศἪᏛⓗኚ໬ 㸵㸫㸲㸬ග⥺ฎ⌮ἲࢆᐇ᪋ࡋࡓ ṓ㤿࡟࠾ࡅࡿෆศἪᏛⓗኚ໬ ㅰ㎡ ㅰ㎡ ㅰ㎡ ㅰ㎡࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭ ཧ⪃ᩥ⊩ ཧ⪃ᩥ⊩ ཧ⪃ᩥ⊩ ཧ⪃ᩥ⊩࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭࣭

(9)

1

㸯❶

㸯❶ ⥴ㄽ

⥴ㄽ

㸯㸫㸯㸬㍍✀㤿⏕⏘ᆅ࡟࠾ࡅࡿ⌧≧ 㸯㸫㸯㸬㍍✀㤿⏕⏘ᆅ࡟࠾ࡅࡿ⌧≧ 㸯㸫㸯㸬㍍✀㤿⏕⏘ᆅ࡟࠾ࡅࡿ⌧≧ 㸯㸫㸯㸬㍍✀㤿⏕⏘ᆅ࡟࠾ࡅࡿ⌧≧ ໭ᾏ㐨࡛ࡣ඲ᅜࡢ⣙ ࡛࠶ࡿ⣙ 㢌ࡢ㍍✀㤿ࡀẖᖺ⏕⏘ࡉࢀ࡚࠸ࡿࠋ⏕ ⏘⪅ࡣ ᖺ ⏘ࢧ࢖ࢡࣝࢆ⥔ᣢࡍࡿࡇ࡜ࢆ┠ᶆ࡜ࡋ࡚⏕⏘ࢆ⾜ࡗ࡚࠸ࡿࡀࠊ᪥ 㧗ᆅ᪉ࡢ㍍✀㤿ࡢ᪩ᮇ⬇Ṛ⁛ࡣ ࠊᦆ⪖⋡ࡣ ࡜ሗ࿌ࡉࢀ࡚࠸ࡿ 㸦0L\DNRVKLHWDO㸧ࠋ ᪥㧗ᆅ᪉ࡢ㍍✀㤿ࡢὶ⏘ࡢෆヂࢆࡳࡿ࡜ࠊ㤿㰯⫵⅖ࡸ㤿ࣃࣛࢳࣇࢫࠊ⫾┙⅖ ➼ࡢឤᰁᛶὶ⏘ࡣ ࠊᚠ⎔㞀ᐖࡸከ⫾ࠊወᙧ➼ࡢ㠀ឤᰁᛶὶ⏘ࡣ ࡛࠶ ࡾࠊཎᅉࡀ≉ᐃࡉࢀࡓ๭ྜࡣ ࡛࠶ࡾࠊཎᅉࡀ≉ᐃ࡛ࡁ࡞࠸๭ྜࡣ ࡛ ࠶ࡗࡓ㸦ᐑ⃝ࡽࠊ㸧ࠋ ẕ࣐࢘ࡢዷፎ ᪥௨㝆࡟ࠊ⾑୰࢚ࢫࢺࣟࢪ࢙ࣥཬࡧࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘ ࢆ ᐃࡍࡿࡇ࡜࡛␗ᖖዷፎࢆ᪩ᮇ࡟Ⓨぢ࡛ࡁࡿࡇ࡜ࡀሗ࿌ࡉࢀ࡚࠸ࡿ 㸦6KLNLFKLHWDO㸧ࠋ⫾Ꮚ➼࡟␗ᖖࡀ࠶ࡿ࡜ࠊẕ㤿ࡢ⾑୰࢚ࢫࢺࣟࢪ࢙ ࣥ⃰ᗘࡢపୗࠊࡲࡓࡣ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡢୖ᪼ࠊࡶࡋࡃࡣࠊࡑࡢ୧᪉ ࡀㄆࡵࡽࢀࡿࡇ࡜࠿ࡽࠊዷፎ୰ࡢ⫾Ꮚࡢ␗ᖖデ᩿࡟ᛂ⏝ࡉࢀ࡚࠸ࡿ㸦6KLNLFKLHW DO㸧ࠋ ࡇࡢࡼ࠺࡞㤿⏕⏘⌧ሙ࡛ࡢ⌧≧ࡢ୰ࠊࢧࣛࣈࣞࢵࢻ✀㞤㤿ࡢ᤼༸ࠊዷፎࠊศ ፔࠊἪஙࡢ᏶඲⏕Ṫ࿘ᮇࢆ㏻ࡋ࡚⏕ṪෆศἪᏛⓗ≉ᚩࢆ᫂ࡽ࠿࡟ࡍࡿࡇ࡜ࡣࠊ ࣐࢘ࡢ⏕⏘࣭⟶⌮ୖࠊᴟࡵ࡚㔜せ࡛࠶ࡿࠋ 㸯㸫㸰㸬㞤࣐࢘ࡢ⦾Ṫ≉ᛶ 㸯㸫㸰㸬㞤࣐࢘ࡢ⦾Ṫ≉ᛶ 㸯㸫㸰㸬㞤࣐࢘ࡢ⦾Ṫ≉ᛶ 㸯㸫㸰㸬㞤࣐࢘ࡢ⦾Ṫ≉ᛶ ࣐࢘ࡣ᫂ᮇࡢ㛗࠸㛗᪥ᮇ࡟ᛶ⭢ࡀ㈿ά໬ࡋ࡚⦾Ṫྍ⬟࡜࡞ࡿ඾ᆺⓗ࡞㛗᪥Ꮨ ⠇⦾Ṫື≀࡛࠶ࡿࠋ㏫࡟ࠊ᫂ᮇࡢ▷࠸▷᪥ᮇ࡟ࡣⓎ᝟࿘ᮇࡀ೵Ṇࡍࡿࠋ୍⯡ⓗ ࡟ࠊ㞤࣐࢘ࡢⓎ᝟࿘ᮇࡣࠊᖹᆒ ᪥㛫࡛࠶ࡾࠊⓎ᝟ࡣ⣙ ᪥㛫⥆ࡁࠊ᤼༸ࡣⓎ

(10)

2 ᝟࿘ᮇ⤊஢ࡢ 㹼 ᪥๓࡟㉳ࡇࡿࠋᚑࡗ࡚ࠊ஺㓄㐺ᮇࡣⓎ᝟ᚩೃࡀ⌧ࢀ࡚࠿ࡽ 㹼 ᪥ᚋࡀ᭱㐺࡜ࡉࢀ࡚࠸ࡿࠋࡋ࠿ࡋࠊᐇ㝿ࡢⓎ᝟ᣢ⥆᪥ᩘࡣ 㹼 ᪥࡜ኚືࡀ ኱ࡁࡃࠊಶయ࡟ࡼࡗ࡚ࡣⓎ᝟඙ೃࡀᚤᙅ࡛ㄆ㆑ࡋ࡟ࡃ࠸ࡇ࡜ࡶ࠶ࡾࠊⓎ᝟㛤ጞ ᪥࠿ࡽ㉳⟬ࡋ࡚᤼༸᪥ࢆண ࡍࡿࡢࡣᴟࡵ࡚ᅔ㞴࡛࠶ࡿࠋࡲࡓࠊ୍⯡ⓗ࡟့ங 㢮ࡢ㞤࡛ࡣࠊୗᆶయ๓ⴥ࠿ࡽศἪࡉࢀࡿ㯤యᙧᡂ࣍ࣝࣔࣥ㸦/+㸧ࡣࠊ᤼༸ࢆㄏ Ⓨࡍࡿࡓࡵ࡟Ⓨ᝟ᮇࡢᩘ᫬㛫ࡢ㛫࡟୍㐣ᛶ࡟⾑୰࡟኱㔞࡟ᨺฟࡉࢀࡿ /+ ࢧ࣮ࢪ ࡀ㉳ࡁࠊࡑࡢᚋࠊ㹼 ᫬㛫࡛᤼༸ࡍࡿࡀࠊ࣐࡛࢘ࡣ⣙㸯㐌㛫࡟ࢃࡓࡗ࡚ /+ ⃰ ᗘࡀୖ᪼࠾ࡼࡧୗ㝆ࡍࡿ≉᭷ࡢ /+ ศἪࣃࢱ࣮ࣥࢆ♧ࡋࠊ࠿ࡘୖ᪼ࡋࡓ⾑୰ /+ ⃰ᗘࡀᇶᗏ್ࡢ 㹼 ಸ⛬ᗘ࡜ప࠸ࡇ࡜࠿ࡽ㸦:KLWPRUHHWDO0LOOHUHW DO1DJDPLQHHWDO㸧ࠊ/+ ࢧ࣮ࢪࢆᇶ‽࡟᤼༸ࢆண ࡍࡿࡇ࡜ࡣ㞴 ࡋ࠸ࠋ ࣐࢘ࡢ༸ᕢࡣࠊ௚ࡢ့ங㢮࡜ࡣ␗࡞ࡾ༸ᕢࡢ⓶㉁࡜㧊㉁ࡀ㏫㌿ࡋ࡚࠾ࡾࠊ᤼ ༸ࡣ᤼༸❐࡜࿧ࡤࢀࡿ༸ᕢࡢ୍㒊ࡢࡳ࠿ࡽ㉳ࡇࡿࠋ࣐࡛࢘ࡣࠊⓎ⫱ࡍࡿ༸⬊ࡢ ኱ࡁࡉࡣ௚ࡢ့ங㢮࡜ẚ࡭࡚ⴭࡋࡃ኱ࡁࡃࠊ᤼༸┤๓ࡢᡂ⇍༸⬊ࡢ┤ᚄࡣࠊయ 㔜ࡀ࡯࡯➼ࡋ࠸࢘ࢩ࡛ࡣ⣙ 㹼FP ࡛࠶ࡿࡢ࡟ᑐࡋࠊ࣐࡛࢘ࡣ⣙ 㹼FP ࡟ࡶ㐩 ࡍࡿࠋ┤⭠᳨ᰝ࡛ࡣࠊ᤼༸❐ࡀ㛤኱ࡋ࡚༸⬊࡟ࡼࡗ࡚༨ࡵࡽࢀ࡚⭾㝯ࡍࡿࡇ࡜ ࢆࡶࡗ࡚᤼༸ࡀ㏆ࡃࠊ஺㓄㐺ᮇ࡟࠶ࡿ࡜ุ᩿ࡍࡿࠋ ̿㸱㸬࣐࢘ࡢዷፎᮇ࡟࠾ࡅࡿ⏕Ṫ⏕⌮Ꮫⓗ≉ᛶ̿㸱㸬࣐࢘ࡢዷፎᮇ࡟࠾ࡅࡿ⏕Ṫ⏕⌮Ꮫⓗ≉ᛶ̿㸱㸬࣐࢘ࡢዷፎᮇ࡟࠾ࡅࡿ⏕Ṫ⏕⌮Ꮫⓗ≉ᛶ̿㸱㸬࣐࢘ࡢዷፎᮇ࡟࠾ࡅࡿ⏕Ṫ⏕⌮Ꮫⓗ≉ᛶ ࣐࢘ࡢዷፎᮇ㛫ࡣ⣙ ᪥࡜㛗࠸ࠋ࣐࢘ࡢዷፎ୰࡟ࡣࠊ࢘ࢩࡸࣄࢶࢪࠊࣈࢱ ࡞࡝௚ࡢᐙ␆࡟ࡣぢࡽࢀ࡞࠸≉ᚩⓗ࡞⌧㇟ࡀㄆࡵࡽࢀࡿࠋ࣐࢘ࡣࠊᐙ␆ࡢ୰࡛ ࡣ╔ᗋ᫬ᮇࡀ᭱ࡶ㐜࠸ࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿࠋࣄࢶࢪࡸࣈࢱ࡛ࡣࠊཷ⢭ᡂ❧ᚋ 㹼 ᪥࡟ࡣ╔ᗋࡀ᏶஢ࡍࡿࠋࡋ࠿ࡋࠊ࣐࡛࢘ࡣࠊཷ⢭ࡋࡓ༸ࡢࡳࡀ᤼༸ᚋ 㹼 ᪥࡟༸⟶࠿ࡽᏊᐑ࡬࡜⛣⾜ࡋ㸦%DWWXWHWDO:HEHOHWDO㸧ࠊᏊ ᐑ࡟฿㐩ࡋࡓཷ⫾⏘≀㸦FRQFHSWXV㸧ࡣ௚ࡢື≀࡛ぢࡽࢀࡿࡼ࠺࡞ᴃ෇ᙧ࡟ఙᒎ

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3 ࡟ࡍࡿࡇ࡜ࡣ࡞ࡃࠊ⌫ᙧࢆࡋ࡚࠾ࡾࠊᙎຊᛶࡢ࠶ࡿ୔ኵ࡞⭷㸦EODVWRF\VWFDSVXOH㸧 ࡟ໟࡲࢀ࡚㸦%HWWHULGJH㸧ࠊᏊᐑෆࢆ⛣ືࡍࡿ㸦*LQWKHU㸧ࠋ ࡑࡢᚋࠊ ᪥๓ᚋ࡟Ꮚᐑ࡟ᐃ╔㸦IL[DWLRQ㸧ࡋ㸦*LQWKHU9DQ1LHNHUN 㸧ࠊ⫾┙ᙧᡂࡣࠊ ᪥ࡈࢁ࠿ࡽ㛤ጞࡍࡿ࡜⪃࠼ࡽࢀ࡚࠸ࡿ㸦$OOHQHWDO 㸧ࠋ⫾┙ᙧᡂࡀጞࡲࡿ࡜Ꮚᐑෆ⭷࡟Ꮚᐑෆ⭷ᮼ㸦HQGRPHWULDOFXS㸧ࡀᙧᡂ ࡉࢀࠊ࣐࢘⤧ẟᛶᛶ⭢่⃭࣍ࣝࣔࣥ㸦H&*㸧ࡀศἪࡉࢀጞࡵࡿ㸦&OHJJHWDO $OOHQ㸧ࠋH&* ࡣࠊẕ࣐࢘ࡢ༸ᕢ࡟༸⬊Ⓨ⫱ࢆಁࡋࠊࡑࡢ༸⬊ࢆ᤼༸ࡉࡏࡿ ࠿㛢㙐㯤య໬ࡉࡏࡿࡇ࡜࡟ࡼࡾ๪㯤యࢆᙧᡂࡉࡏࡿࠋึᮇࡢዷፎ⥔ᣢࡣࠊࡇࢀ ࡽࡢ๪㯤య࠿ࡽศἪࡉࢀࡿࣉࣟࢪ࢙ࢫࢸࣟࣥ࡟ࡼࡿࡶࡢ࡜⪃࠼ࡽࢀ࡚࠸ࡿࠋ ዷፎ ᪥๓ᚋ࠿ࡽぢࡽࢀࡿ࣐࢘࡟≉␗ⓗ࡞⌧㇟࡜ࡋ࡚ࡣࠊ⫾Ꮚᛶ⭢ࡢ⫧኱ ໬⌧㇟ࡀ࠶ࡆࡽࢀࡿࠋ⫾Ꮚᛶ⭢ࡣ ᪥๓ᚋ࠿ࡽᐜ✚ࠊ㔜㔞ࡀᛴ⃭࡟ቑຍࡋࠊ 㹼 ᪥࡟᭱኱࡟㐩ࡍࡿࠋዷፎ 㹼 ᪥࡟ࡣࠊẕ࣐࢘ࡢ༸ᕢࡼࡾࡶ⫾Ꮚᛶ ⭢ࡀ኱ࡁࡃ⫧኱ࡍࡿࠋࡑࡢᚋࠊ⫾Ꮚᛶ⭢ࡢᐜ✚ࡣࠊ⦰ᑠࡋጞࡵࠊฟ⏕᫬࡟ࡣࣆ ࣮ࢡ᫬ࡢ ศࡢ ࠿ࡽ ศࡢ ࡢ኱ࡁࡉ࡟ࡲ࡛⦰ᑠࡍࡿ㸦&ROHHWDO㸧ࠋ ⫧኱ࡍࡿ⫾Ꮚᛶ⭢ࡣࠊ⫾┙࡟࠾ࡅࡿ࢚ࢫࢺࣟࢪ࢙ࣥࡢ๓㥑≀㉁ࡢศἪ※ 㸦3DVKHQHWDO㸧࡜ࡋ࡚ࡢᙺ๭ࡸ⫾Ꮚ⫾┙ࣘࢽࢵࢺ࡛ࡢࢫࢸࣟ࢖ࢻ௦ㅰ 㸦&KDYDWWHHWDO㸧࡟㛵୚ࡍࡿ࡜⪃࠼ࡽࢀ࡚࠸ࡿࠋࡋ࠿ࡋࠊ⫾Ꮚᛶ⭢࡟ ࠾ࡅࡿ⫧኱⦰ᑠࡢ࣓࢝ࢽࢬ࣒ࡸ⫾Ꮚᛶ⭢ࡀዷፎ࡟ཬࡰࡍᙳ㡪࡞࡝࡟ࡘ࠸࡚ࡣᮍ ࡔ࡟ゎ᫂ࡉࢀ࡚࠸࡞࠸ࠋ 㸯㸫㸲㸬ศፔᚋ᤼༸ 㸯㸫㸲㸬ศፔᚋ᤼༸ 㸯㸫㸲㸬ศፔᚋ᤼༸ 㸯㸫㸲㸬ศፔᚋ᤼༸ ࣐࡛࢘ࡣศፔᚋ⣙ 㐌㛫࡜࠸࠺ࠊᐙ␆ࡢ୰࡛ࡣᴟࡵ࡚᪩࠸᫬ᮇ࡟ศፔᚋࡢึ ᅇⓎ᝟ࢆ♧ࡍࡇ࡜ࡀ⏕Ṫ⏕⌮Ꮫⓗ≉ᚩࡢ㸯ࡘ࡛࠶ࡿࠋ࣐࢘ࡢศፔᚋࡢึᅇⓎ᝟ ࡣࠊࣇ࢛࣮ࣝࣄ࣮ࢺ㸦IRDOKHDW㸧࡜࿧ࡤࢀ࡚࠸ࡿࠋึᅇⓎ᝟࡟࠾ࡅࡿⓎ᝟ᣢ⥆ ᪥ᩘࡣ 㹼 ᪥㛫࡛▷࠸ࡶࡢࡀከࡃࠊࡲࡓึᅇ᤼༸ࡣศፔᚋ 㹼 ᪥ࡢ㛫࡟㉳ࡇ

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4 ࡿࡇ࡜ࡀከ࠸ࠋṇᖖศፔᚋࡢᏊᐑಟ᚟ࡣ᪩ࡃࠊᏊᐑࡢ㏥⦰ࡣึᅇⓎ᝟ࡢึ᪥ࡲ ࡛࡟࡯ࡰ⤊஢ࡍࡿࡀࠊึᅇⓎ᝟᫬ࡢ஺㓄࡟ࡼࡿཷ⫾⋡ࡣ➨ ᅇ௨㝆ࡢⓎ᝟ࡢཷ ⫾⋡ࡼࡾࡶప࠸ࠋᏊᐑෆ⭷ࡢಟ᚟࡟せࡍࡿᮇ㛫ࡣࠊಶయᕪࡀㄆࡵࡽࢀࡿࠋ㸦᳃ࡽ 㸧㸯㸫㸳㸬◊✲┠ⓗ 㸯㸫㸳㸬◊✲┠ⓗ 㸯㸫㸳㸬◊✲┠ⓗ 㸯㸫㸳㸬◊✲┠ⓗ ᮏ◊✲ࡣࠊ᪥ᮏ⏘ࢧࣛࣈࣞࢵࢻ✀㞤࣐࢘ࡢྛ✀⏕Ṫࢫࢸ࣮ࢪ࡟࠾ࡅࡿ⏕Ṫ㛵㐃࣍ࣝࣔࣥࡢศἪኚ໬ࢆ᫂ࡽ࠿࡟ࡋࠊ༸ᕢᶵ⬟ㄪ⠇ᶵᵓࢆ᫂ࡽ࠿࡟ࡍࡿࡇ࡜ࢆ ┠ⓗ࡜ࡋࡓࠋ◊✲࡟㝿ࡋ࡚ࠊ㞤࣐࢘ࡢዷፎᮇ࡟࠾ࡅࡿෆศἪᏛⓗኚ໬ࠊศፔᚋ Ⓨ᝟࡟࠾ࡅࡿෆศἪᏛⓗኚ໬ࠊ᪥ᮏࡢᏘ⠇ኚ໬࡟࠾ࡅࡿྛ✀⏕Ṫࢫࢸ࣮ࢪࡢෆ ศἪᏛⓗኚ໬࡟ࡘ࠸᳨࡚ウࡋࡓࠋࡲࡓࠊᙜṓ㞤࣐࢘࡟ග⥺ฎ⌮ࢆᐇ᪋ࡋࠊࡑࡢ ෆศἪᏛⓗཬࡧᙧែᏛⓗኚ໬࡟ࡘ࠸᳨࡚ウࡋࡓࠋ

(13)

5 㸰❶ 㸰❶㸰❶ 㸰❶ ᮦᩱ࠾ࡼࡧ᪉ἲᮦᩱ࠾ࡼࡧ᪉ἲᮦᩱ࠾ࡼࡧ᪉ἲᮦᩱ࠾ࡼࡧ᪉ἲ ᮏ❶࡛ࡣࠊᮏ◊✲඲య࡟࠾࠸࡚ඹ㏻ࡋ࡚౑⏝ࡋࡓᐇ㦂ᮦᩱ࡞ࡽࡧ࡟᪉ἲࢆグ㏙ࡍࡿࠋಶࠎࡢᐇ㦂࡟࠾࠸࡚ࠊࡑࢀࡒࢀ࡟␗࡞ࡿᮦᩱ࠾ࡼࡧ᪉ἲ࡟ࡘ࠸࡚ᚲせ ࡟ᛂࡌ࡚ྛ❶࡟グ㏙ࡋࡓࠋ 㸰㸫㸯㸬౪ヨື≀ 㸰㸫㸯㸬౪ヨື≀ 㸰㸫㸯㸬౪ヨື≀ 㸰㸫㸯㸬౪ヨື≀ ᐇ㦂㤿࡜ࡋ࡚ࡣࠊࢧࣛࣈࣞࢵࢻ✀㞤࣐࢘ࢆ౑⏝ࡋࡓࠋࡇࢀࡽࡢ࣐࢘ࡣ⮫ᗋⓗ ࡟೺ᗣ࡛ࠊዷፎ㤿࡟࠶ࡗ࡚ࡣṇᖖ࡞ዷፎᮇ㛫ࡢࡢࡕ೺ᗣ࡞Ꮚ㤿ࢆ⮬↛࡟ศፔࡋࠊ 㠀ዷፎ㤿࡟࠶ࡗ࡚ࡣṇᖖⓎ᝟࿘ᮇࢆᅇᖐࡍࡿ࣐࢘ࢆᐇ㦂࡟౪ࡋࡓࠋ 㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ 㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ 㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ 㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ ⾑ᾮࡣࠊ㢁㟼⬦ࡼࡾ࡬ࣃࣜࣥฎ⌮┿✵᥇⾑⟶㸦ࢸࣝࣔࠊᮾி㸧ࢆ⏝࠸࡚⾜ࡗ ࡓࠋ᥇⾑ᚋࡣࠊịୖ࡛ಖᏑࡋࠊ࡛ࡁࡿࡔࡅ㏿ࡸ࠿࡟ Υࠊ* ࡛ ศ㛫㐲ᚰ ࡋ࡚⾑₢ࢆศ㞳ࡋࡓࠋᚓࡽࢀࡓ⾑₢ࡣࠊྛ✀࣍ࣝࣔࣥ ᐃࡲ࡛㸫Υ࡟࡚෾⤖ ಖᏑࡋࡓࠋ 㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖㸦㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖㸦㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖㸦㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖㸦5,$5,$5,$㸧5,$㸧㸧㸧㸰㸫㸱㸫㸯㸬࢖ࣥࣄࣅࣥࡢ 㸰㸫㸱㸫㸯㸬࢖ࣥࣄࣅࣥࡢ 㸰㸫㸱㸫㸯㸬࢖ࣥࣄࣅࣥࡢ 㸰㸫㸱㸫㸯㸬࢖ࣥࣄࣅࣥࡢ 5,$5,$5,$ )LJ5,$ )LJ)LJ)LJ ࢖ࣥࣄࣅࣥࡢ 5,$ ࡣࠊᮾி㎰ᕤ኱Ꮫ⋇་⏕⌮Ꮫ◊✲ᐊ࡛㛤Ⓨࡋࡓ᪉ἲ㸦+DPDGD HWDO㸧࡟ࡼࡗ࡚⾜ࡗࡓࠋ ᐃࡢᡭ㡰࡟ࡘ࠸࡚ࡣࠊ)LJ ࡟ࣇ࣮ࣟࢳࣕ ࣮ࢺࡢᅗࢆグ㍕ࡋࡓࠋ ᐃ࡟ࡣࠊᶆ‽ရ࡜ࡋ࡚࢘ࢩ࢖ࣥࣄࣅࣥ㸦N'D㸧ࢆ⏝࠸ ࡓࠋ➨୍ᢠయ࡟ࡣࠊᢠ࢘ࢩ࢖ࣥࣄࣅࣥ⾑Ύ㸦71'+㸧ࠊ➨஧ᢠయ࡜ࡋ࡚ᢠ࢘ࢧࢠ Țࢢࣟࣈࣜࣥࣖࢠ⾑Ύ㸦㸧ࠊᶆ‽ᢠཎ࡟ࡣࠊࢡ࣑ࣟࣛࣥ 7 ἲ࡟ࡼࡗ࡚ _, ࡛ᶆ㆑ࡋࡓ࢘ࢩ࢖ࣥࣄࣅࣥ㸦N'D㸧ࢆ౑⏝ࡋࡓࠋ࢔ࢵࢭ࢖ෆ࠾ࡼࡧ࢔ࢵࢭ࢖㛫

(14)

6 ኚືࡣࠊࡑࢀࡒࢀ ࠾ࡼࡧ ࡛࠶ࡗࡓࠋ 㸰㸫㸱㸫㸰㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦㸰㸫㸱㸫㸰㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦㸰㸫㸱㸫㸰㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦㸰㸫㸱㸫㸰㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦/+/+/+㸧ཬࡧ༸⬊่⃭࣍ࣝࣔࣥ㸦/+㸧ཬࡧ༸⬊่⃭࣍ࣝࣔࣥ㸦㸧ཬࡧ༸⬊่⃭࣍ࣝࣔࣥ㸦㸧ཬࡧ༸⬊่⃭࣍ࣝࣔࣥ㸦)6+)6+㸧)6+)6+㸧㸧㸧ࡢࡢࡢ 5,$ࡢ 5,$5,$5,$ )LJ )LJ )LJ )LJ ᐃࡢᡭ㡰࡟ࡘ࠸࡚ࡣࠊ)LJ ࡟ࣇ࣮ࣟࢳ࣮ࣕࢺࡢᅗࢆグ㍕ࡋࡓࠋ/+ ࠾ࡼ ࡧ )6+ ࡢ ᐃ࡟ࡣᶆ‽ရ࠾ࡼࡧ➨୍ᢠయ࡜ࡋ࡚ 1DWLRQDO,QVWLWXWHRI'LDEHWHV 'LJHVWLYHDQG.LGQH\'LVHDVHV1,''.࠿ࡽᥦ౪ࡉࢀࡓ࣐࢘ /+$)3$࠾ࡼ ࡧ ࢘ ࣐ )6+$)3% ࠊ ࢘ ࣐ /+ ᢠ య $)3 ࠾ ࡼ ࡧ ࢘ ࣐ )6+ ᢠ య $)3ࢆ⏝࠸ࡓࠋ➨஧ᢠయ࡜ࡋ࡚ࡣࠊᢠ࢘ࢧࢠȚࢢࣟࣈࣜࣥࣖࢠ⾑Ύ ࢆࠊᶆ‽ᢠཎ࡟ࡣࢡ࣑ࣟࣛࣥ 7 ἲ࡟ࡼࡗ࡚ _{, ࡛ᶆ㆑ࡋࡓ࣐࢘} /+$)3$࠾ࡼࡧ࣐࢘ )6+$)3%ࢆ౑⏝ࡋࡓࠋ࢔ࢵࢭ࢖ෆ࠾ࡼࡧ࢔ࢵࢭ ࢖㛫ኚືࡣࠊ/+ ࡀ ࠾ࡼࡧ ࠊ)6+ ࡀ ࠾ࡼࡧ ࡛࠶ࡗࡓࠋ 㸰㸫㸱㸫㸱㸬ࣉࣟࣛࢡࢳࣥࡢ 㸰㸫㸱㸫㸱㸬ࣉࣟࣛࢡࢳࣥࡢ 㸰㸫㸱㸫㸱㸬ࣉࣟࣛࢡࢳࣥࡢ 㸰㸫㸱㸫㸱㸬ࣉࣟࣛࢡࢳࣥࡢ 5,$5,$5,$ )LJ5,$ )LJ)LJ)LJ ᐃࡢᡭ㡰࡟ࡘ࠸࡚ࡣࠊ)LJ ࡟ࣇ࣮ࣟࢳ࣮ࣕࢺࡢᅗࢆグ㍕ࡋࡓࠋࣉࣟࣛࢡ ࢳࣥ㸦35/㸧ࡢ ᐃ࡟ࡣࠊᶆ‽ရ࠾ࡼࡧ➨୍ᢠయ࡜ࡋ࡚ $)3DUORZ ༤ኈ㸦3LWXLWDU\ KRUPRQHVDQGDQWLVHUDFHQWHU+DUERU8&/$PHGLFDOFHQWHU&DOLIRUQLD86$㸧 ࠿ࡽᥦ౪ࡉࢀࡓ࣐࢘ 35/ ࠾ࡼࡧᢠ࣐࢘ 35/ ࣛࢵࢺ⾑Ύࢆ⏝࠸ࡓࠋ➨஧ᢠయ࡜ࡋ ࡚ࡣࠊᢠࣛࢵࢺȚࢢࣟࣈࣜࣥࣖࢠ⾑Ύࢆࠊᶆ‽ᢠཎ࡟ࡣࢡ࣑ࣟࣛࣥ 7 ἲ࡟ࡼࡗ ࡚_{, ࡛ᶆ㆑ࡋࡓ࣐࢘ 35/ ࢆ౑⏝ࡋࡓࠋ࢔ࢵࢭ࢖ෆ࠾ࡼࡧ࢔ࢵࢭ࢖㛫ኚືࡣࠊ} ࠾ࡼࡧ ࡛࠶ࡗࡓࠋ 㸰㸫㸱㸫㸲㸬ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢ 㸰㸫㸱㸫㸲㸬ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢ 㸰㸫㸱㸫㸲㸬ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢ 㸰㸫㸱㸫㸲㸬ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢ 5,$5,$5,$5,$ )LJ)LJ)LJ)LJ ᐃࡢᡭ㡰࡟ࡘ࠸࡚ࡣࠊ)LJ ࡟ࣇ࣮ࣟࢳ࣮ࣕࢺࡢᅗࢆグ㍕ࡋࡓࠋࢫࢸࣟ ࢖ࢻ࣍ࣝࣔࣥ㸦ࢸࢫࢺࢫࢸࣟࣥࠊ࢚ࢫࢺࣛࢪ࣮࢜ࣝ ș㸧ࡢ ᐃࡣࠊ⏣㇂ࡽ㸦7D\D

(15)

7 HWDO㸧࠾ࡼࡧỌ⏣ࡽ㸦1DJDWDHWDO㸧࡟ࡼࡿ᪉ἲ࡟ࡼࡗ࡚⾜ࡗࡓࠋ ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ ᐃࡢࡓࡵࡢࢧࣥࣉࣝࡣࠊࢪ࢚ࢳ࢚࣮ࣝࢸࣝ㸦ࢻ࣮ࢱ࢖ࢺࠊ ྠோ໬Ꮫ ⇃ᮏࠊ᪥ᮏ㸧࡟ࡼࡿ ᅇᢳฟἲ࡟ࡼࡾࠊࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࢆᢳฟ ࡋࡓࠋ ᐃ࡟ࡣࠊ➨୍ᢠయ࡜ࡋ࡚ *'1LVZHQGHU ༤ኈ㸦&RORUDGR6WDWH8QLYHUVLW\ )RUW&ROLQV&286$㸧࠿ࡽ౪୚ࡉࢀࡓᢠࢸࢫࢺࢫࢸࣟࣥ*'112ࠊᢠ࢚ ࢫࢺࣛࢪ࣮࢜ࣝșᢠయ*'11Rࢆ౑⏝ࡋࡓࠋᶆ㆑ᢠཎ࡜ࡋ࡚ࡣࠊ࢔࣐ࢩ ࣒ࣕ♫〇 WHVWRVWHURQH2FDUER[\PHWK\OR[LQR>_{,@LRGRKLVWDPLQH} ,0ࠊ2HVWUDGLRO2FDUER[\PHWK\OR[LQR>_{,@LRGRKLVWDPLQHࢆ} ⏝࠸ࡓࠋ➨஧ᢠయ࡜ࡋ࡚ࡣࠊᢠࣄࢶࢪȚࢢࣟࣈࣜࣥࣟࣂ⾑Ύ㸦ࢣ࣑ࢥࣥ&$86$㸧 ࢆ⏝࠸ࡓࠋྛࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢ࢔ࢵࢭ࢖ෆ࠾ࡼࡧ࢔ࢵࢭ࢖㛫ኚືࡣࠊࢸࢫ ࢺࢫࢸࣟࣥࡀ ࠾ࡼࡧ ࠊ࢚ࢫࢺࣛࢪ࣮࢜ࣝșࡀ ࠾ࡼࡧ ࡛࠶ࡗࡓࠋ 㸰㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥࡢ᫬㛫ศゎ⺯ගච␿ ᐃἲ㸦㸰㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥࡢ᫬㛫ศゎ⺯ගච␿ ᐃἲ㸦㸰㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥࡢ᫬㛫ศゎ⺯ගච␿ ᐃἲ㸦㸰㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥࡢ᫬㛫ศゎ⺯ගච␿ ᐃἲ㸦75757575),$),$),$),$㸧㸧㸧㸧 ࣉ ࣟ ࢪ ࢙ ࢫ ࢸ ࣟ ࣥ ࡣ ࠊ '(/),$ SURJHVWHURQH ᐃ NLW :DOODF '(/),$ 3URJHVWHURQH5HDJHQWV㸪ࣃ࣮࢚࣐࣮࢟ࣥࣝࣛ࢖ࣇࢧ࢖࢚ࣥࢫࢪࣕࣃࣥᰴ㸪ᮾ ிࢆ⏝࠸ࡓ➇ྜⓗ᫬㛫ศゎ⺯ගච␿ ᐃἲ75),$࡟ࡼࡾ඲⾜⛬ ᫬㛫࡛ ᐃࡋࡓࡍ࡞ࢃࡕ㸪ࣉࣟࢪ࢙ࢫࢸࣟࣥᶆ‽ᾮ࠶ࡿ࠸ࡣ⾑Ύࢧࣥࣉࣝ ȣO ࢆ࣐࢖ ࢡࣟࣉ࣮ࣞࢺ࡟ศὀࡋ㸪⥆࠸࡚ᢠࣉࣟࢪ࢙ࢫࢸࣟࣥᢠయ࠾ࡼࡧ࣮ࣘࣟࣆ࣒࢘ᶆ ㆑ࡉࢀࡓࣉࣟࢪ࢙ࢫࢸࣟࣥࣜ࢞ࣥࢻࢆ㸪ࡑࢀࡒࢀ ȣO ῧຍࡋ㸪 ᫬㛫ࡢ᧠⚈ ࢆ⾜ࡗࡓ࣐࢖ࢡࣟࣉ࣮ࣞࢺࢆὙίࡋ㸪ቑᙉヨ⸆ ȣO ࢆῧຍࡋࡓᚋ㸪࣐ࣝࢳ ࣛ࣋ࣝࣉ࣮ࣞࢺ࢝࢘ࣥࢱ࣮:DOODF$5920;㸪ᰴࣃ࣮࢚࣐࣮࢟ࣥࣝࢪࣕ ࣃࣥ㸪⚄ዉᕝ࡟ࡼࡾ᫬㛫ศゎ⺯ග ᐃࢆ⾜࠸㸪᳨㔞⥺ࡢసᡂ㸪࡞ࡽࡧ࡟ᮍ▱ヨ ᩱࡢ⃰ᗘゎᯒࢆ⾜ࡗࡓࡍ࡭࡚ࡢ⾑Ύࢧࣥࣉࣝࡣ 㔜 ᐃ࡟࡚⾜ࡗࡓ

(16)

8

Flow chart of the radioimmunoassay (RIA) for equine inhibin

(1) 100 μl standard preparation in 0.05 M phosphate buffered saline(PBS) containing 5% bovine serum albumin (BSA).

(2) 100 μl female serum or

(1) 100 μl unknown sample preparations. (2) 50 μl 0.05 M PBS containing 5% BSA. ЎЎЎЎ

(3) 50 μl anti bovine inhibin (TNDH-1 ; 1:40000) in 0.05 M PBS

containing 0.4% normal rabbit serum(NRS) and 0.05 M ethylendiamine- N,N,N’,N’-tetraacetic acid, disodium salt (EDTA).

Ў Ў Ў Ў (4) Incubation at 32Υ for 24 h. Ў Ў Ў Ў

(5) 50 μl 125I-32 KDa bovine inhibin in 0.05 M PBS containing 0.15% Bacitracin, 5 mM methionin and 0.1% 3-[(3-Cholamidopropyl)-dimethylammonio]-1-

propane sulfonate (CHAPS).

ЎЎ ЎЎ

(6) Incubation at 32Υfor 24h. ЎЎЎЎ

(7) 50 μl anti-rabbit γ-globulin diluted with 0.05 M PBS containing 5% Polyethyleneglycol(PEG) ЎЎ ЎЎ (8) Incubation at 4Υfor 24h. ЎЎЎЎ

(9) Centrifugation at 1700G for 30 min 4Υ.

(10) Decanting supernatant and swabbing extra drop. (11) Counting radioactivity of precipitate with a γ-counter.

Fig.2-1. Procedure of RIA for equine inhibin.

(17)

9

Flow chart of the radioimmunoassay (RIA) for equine LH and FSH (1)100 μl standard preparation in 0.05 M (PBS) containing 1% BSA or

50 μl sample preparations.

(2) 50 μl 0.05 M PBS containing 1% BSA. ЎЎЎЎ

(3) 50 μl anti-equine LH(NIDDK anti-equine LH (AFP-240580), 1:100,000) or anti-equine FSH (NIDDK anti-equine FSH (AFP-2062096) in 0.05 M PBS containing 0.4% NRS and 0.05 M EDTA.

(5 )50 μl 125_{I-equine LH (provided by NIDDK) or}125_{I-equine FSH (provided by} NIDDK) in 0.05 M PBS containing 1% BSA.

ЎЎ ЎЎ

(6) Incubation at 4Υfor 24 h. ЎЎЎЎ

(7) 50 μl anti-rabbit γ-globulin diluted with 0.05 M PBS containing 5% PEG. ЎЎ ЎЎ

(8) Incubation at 4Υfor 24 h.

ЎЎЎЎ

(9) Centrifugation at 1700 G for 30 min 4Υ.

(10) Decanting supernatant and swabbing extra drop. (11 )Counting radioactivity of precipitate with a γ-counter.

(18)

10

Flow chart of the radioimmunoassay (RIA) for equine PRL

(1) 100 μl standard preparation in 0.05 M (PBS) containing 5% BSA or 50 μl sample preparations.

(2) 50 μl 0.05M PBS containing 5% BSA. ЎЎЎЎ

(3) 50 μl anti-equine PRL (provided by Dr. A.F.Parlow, 1:15,000) in 0.05 M PBS containing 0.4% normal rat serum and 0.05 M EDTA.

(5) 50 μl 125I-equine PRL (provided by Dr. A.F.Parlow) in 0.05 M PBS containing 5% BSA.

ЎЎ ЎЎ

(6) Incubation at 4Υfor 24 h. ЎЎЎЎ

(7) 50 μl anti-rat γ-globulin diluted with 0.05 M PBS containing 5% PEG. ЎЎ ЎЎ

(8) Incubation at 4Υfor 24 h.

ЎЎЎЎ

(9) Centrifugation at 1700 G for 30 min 4Υ.

Fig.2-3. Procedure of RIA for equine PRL.

(19)

11

Flow chart of the RIA for steroid hormones-1

(1 )Extraction

(1) 400 μl standard preparations or sample preparations diluted with water. (2) 2 ml anhydrous ethyl ether.

ЎЎЎЎ

(3) Mixing for 3 min ЎЎЎЎ

(4) Freezing water phase with dryice ethnol. ЎЎЎЎ

(5) Decanting the ether phase to another tube. ЎЎЎЎ

(6) Drying down the ether phase at 50Υ. ЎЎЎЎ

(7) Rinsing the wall of tube with ether. ЎЎЎЎ

(8) Drying down the ether phase at 50Υ. Ў

Ў Ў Ў

(9) 400 μl 0.05 M PBS containing 1% BSA and mixing for 3 min.

(20)

12

Flow chart of the RIA for steroid hormone – 2 (2) Assay Procedure after extraction

(10) Aliquoting for 100 μl to another tube. Ў

Ў Ў Ў

(11) 100 μl anti-oestradiol (GDN 244; 1:600,000) or testosterone (GDN 250; 1:400,000) in 0.05 M PBS containing 0.25% normal seep serum and 0.05 M EDTA.

Ў Ў Ў Ў

(12) 100 μl 125_{I-labeled each radioligand in 0.05 M PBS containing 1% BSA.}

Ў Ў Ў Ў (13) Incubation for 24 h at 4Υ Ў Ў Ў Ў

(14 )100 μl anti-sheep γ-globulin diluted with 0.05 M PBS containing 5% PEG. Ў Ў Ў Ў (15) Incubation at 4Υfor 24 h. Ў Ў Ў Ў

(16) Centrifugation at 170 0G for 30 min 4Υ.

(21)

13 㸱❶ 㸱❶㸱❶ 㸱❶ ࢧࣛࣈࣞࢵࢻ✀࡟࠾ࡅࡿዷፎᮇࡢෆศἪᏛⓗኚ໬ࢧࣛࣈࣞࢵࢻ✀࡟࠾ࡅࡿዷፎᮇࡢෆศἪᏛⓗኚ໬ࢧࣛࣈࣞࢵࢻ✀࡟࠾ࡅࡿዷፎᮇࡢෆศἪᏛⓗኚ໬ࢧࣛࣈࣞࢵࢻ✀࡟࠾ࡅࡿዷፎᮇࡢෆศἪᏛⓗኚ໬ 㸱㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ 㸱㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ 㸱㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ 㸱㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ ࣐࢘ࡢዷፎᮇ࡟ࡣ࠸ࡃࡘ࠿ࡢ≉ᚩࡀ࠶ࡿࠋ࣐࢘ࡢዷፎᮇ㛫ࡣ⣙ ᪥࡛࠶ࡾࠊ ⣙ ᪥࡛╔ᗋࡋ࡚Ꮚᐑෆ⭷ᮼࢆᙧᡂࡋࠊ࣐࢘⤧ẟᛶᛶ⭢่⃭࣍ࣝࣔࣥ㸦H&*㸧 ࢆศἪࡍࡿࠋH&* ࡢస⏝࡟ࡼࡾࠊẕ࣐࢘༸ᕢ࡟Ⓨ⫱ࡋࡓ༸⬊ࡣ㯤య໬ࡋࠊ๪㯤య ࢆᙧᡂࡍࡿࠋࡲࡓࠊዷፎᮇ୰ᮇ࠿ࡽᚋᮇ࡟࠿ࡅ࡚ࠊ⫾Ꮚࡢᛶ⭢ࡀⴭࡋࡃ⫧኱ࡍ ࡿ⌧㇟ࡀ▱ࡽࢀ࡚࠸ࡿ㸦⏣୰㸧ࠋ᭦࡟ࠊศፔᚋ࡟ࡣࠊ᪩ᮇ࡟Ⓨ᝟ࡀᅇᖐࡋࠊ ศፔᚋ ᪥ࡈࢁ࡟ࡣ᤼༸ࡀㄆࡵࡽࢀࡿ࡞࡝ࠊ࣐࢘ࡣࠊ௚ࡢ့ங㢮࡟ࡣぢࡽࢀ࡞ ࠸⏕Ṫ⏕⌮Ꮫⓗ≉ᚩࢆ᭷ࡋ࡚࠸ࡿࠋ ࡇࡢࡼ࠺࡞࣐࢘≉᭷ࡢዷፎ⥔ᣢᶵᵓࢆ⌮ゎࡍࡿࡓࡵ࡟ࡣࠊዷፎ඲ᮇ㛫ࢆ㏻ࡋ࡚ ⏕ṪෆศἪᏛⓗ࡞ゎᯒࡀᚲせ࡛࠶ࡿࠋࡇࢀࡲ࡛ࠊከࡃࡢ◊✲⪅࡟ࡼࡾዷፎ୰ࡢ ࣐࢘ࡢᛶ⭢่⃭࣍ࣝࣔࣥࡸࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢ ᐃ⤖ᯝࡀሗ࿌ࡉࢀ࡚ࡁࡓࡀࠊ ዷፎ඲ᮇ㛫ࢆ㏻ࡋ࡚ྠ୍ࡢࢧࣥࣉࣝ୰ࡢ࣍ࣝࣔࣥࢆ ᐃࡋࡓሗ࿌ࡣᑡ࡞࠸ࠋᮏ ◊✲࡛ࡣࠊ࣐࢘ዷፎᮇ㛫୰ࡢ⏕ṪෆศἪኚ໬ࢆ᫂ࡽ࠿࡟ࡍࡿࡇ࡜ࢆ┠ⓗ࡜ࡋ࡚ࠊ ࢧࣛࣈࣞࢵࢻ✀㞤࣐࢘ࡢ඲ዷፎᮇ㛫୰ࡢ ✀㢮ࡢ⏕Ṫ㛵㐃࣍ࣝࣔࣥࢆ ᐃࡋࡓࠋ

(22)

14 㸱㸫㸰㸬㸱㸫㸰㸬㸱㸫㸰㸬㸱㸫㸰㸬 ᮦᩱ࠾ࡼࡧ᪉ἲᮦᩱ࠾ࡼࡧ᪉ἲᮦᩱ࠾ࡼࡧ᪉ἲᮦᩱ࠾ࡼࡧ᪉ἲ 㸱㸫㸰㸫㸯㸬౪ヨື≀ 㸱㸫㸰㸫㸯㸬౪ヨື≀ 㸱㸫㸰㸫㸯㸬౪ヨື≀ 㸱㸫㸰㸫㸯㸬౪ヨື≀ ᖺ࠿ࡽ ᖺࡲ࡛ࠊ໭ᾏ㐨ࡢ -5$ ᪥㧗⫱ᡂ∾ሙ࡛㣫㣴ࡉࢀ࡚࠸ࡓࠊ⮫ᗋ ⓗ࡟ṇᖖ࡞ࢧࣛࣈࣞࢵࢻ✀ࡢ㞤࣐࢘ 㢌㸦㹼 ṓ㸧ࢆ⏝࠸ࡓࠋṇᖖ࡞ዷፎ࣭ ศፔࢆ♧ࡋࡓࠊ ᅇࡢዷፎࢆᮏ◊✲࡟౑⏝ࡋࡓࠋ࡞࠾ࠊศፔࡋࡓᏊ㤿ࡢᛶูࡢ ෆヂࡣࠊ㞝ࡀ 㢌ࠊ㞤ࡀ 㢌࡛࠶ࡗࡓࠋᮏ◊✲࡛⏝࠸ࡓ࣐࢘ࡢዷፎᮇ㛫ࡢᖹᆒ ࡣࠊs ᪥㸦Q 㸧࡛࠶ࡗࡓࠋ 㸱㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ 㸱㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ 㸱㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ 㸱㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ ᭱⤊஺㓄᪥ࢆࠊዷፎ ᪥࡜ࡋ࡚ࠊዷፎ඲ᮇ㛫ࢆ㏻ࡋ࡚㸯㐌㛫㛫㝸࡛㢁㟼⬦࠿ ࡽ࡬ࣃࣜࣥฎ⌮┿✵᥇⾑⟶㸦ࢸࣝࣔࠊᮾிࠊ᪥ᮏ㸧ࢆ⏝࠸࡚᥇⾑ࡋࡓࠋ 㸱㸫㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢ ᐃ 㸱㸫㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢ ᐃ 㸱㸫㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢ ᐃ 㸱㸫㸰㸫㸱㸬ྛ✀࣍ࣝࣔࣥࡢ ᐃ ➨ ❶࡟グ㏙ࡋࡓ ᐃ᪉ἲࢆ⏝࠸࡚ࠊ⾑୰㯤యᙧᡂ࣍ࣝࣔࣥ㸦/+㸧ࠊ༸⬊่⃭ ࣍ࣝࣔࣥ㸦)6+㸧ࠊࣉࣟࣛࢡࢳࣥࠊ࢚ࢫࢺࣛࢪ࣮࢜ࣝࠊࢸࢫࢺࢫࢸࣟࣥࠊ࢖ࣥࣄ ࣅࣥ⃰ᗘࢆࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖㸦5,$㸧ἲ࡟࡚ࠊࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࢆ᫬㛫 ศゎ⺯ගච␿ ᐃἲ㸦75),$㸧࡟࡚ ᐃࡋࡓࠋ 㸱㸫㸰㸫㸲㸬⤫ィฎ⌮ 㸱㸫㸰㸫㸲㸬⤫ィฎ⌮ 㸱㸫㸰㸫㸲㸬⤫ィฎ⌮ 㸱㸫㸰㸫㸲㸬⤫ィฎ⌮ ⤖ᯝࡣࠊᖹᆒsᶆ‽ㄗᕪ࡛♧ࡍࠋዷፎᮇ㛫ࢆ⤫୍ࡍࡿࡓࡵ࡟ࠊ᭱⤊஺㓄᪥ࢆ 㐌ࠊศፔ᪥ࢆ ࡜ 㐌ࡢ㛫࡟タᐃࡋࠊ 㐌┠࡛ศ๭ࡋࠊཷ⫾᪥࠿ࡽ 㐌┠ࡲ ࡛࡜ 㐌┠࠿ࡽศፔ᪥ࡲ࡛࡟ศࡅ࡚ㄪᩚࡋ࡚ࢢࣛࣇࢆసᡂࡋࡓࠋྛ⾑୰࣍ࣝࣔ ࣥ⃰ᗘ࡟ࡘ࠸࡚ࡣࠊ ඖ㓄⨨ࡢศᩓศᯒἲࡢࡕ࡟ 3RVW+RF ࢆࡶࡕ࠸࡚᭷ពᛶࢆ ᳨ᐃࡋࡓࠋ ✀㢮ࡢࢹ࣮ࢱࡢ┦㛵ᛶࢆㄪ࡭ࡿ࡟ࡣࠊ᭱ᑠ஧஌ἲ࡟ࡼࡾᅇᖐ┤⥺ᘧ

(23)

15 ࢆồࡵࠊࣆ࢔ࢯࣥࡢ┦㛵ಀᩘࢆồࡵ࡚┦㛵ࡢ᭷ពᛶࢆ᳨ᐃࡋࡓࠋ ඲࡚ࡢ⤫ィฎ⌮࡟㛵ࡋ࡚ࠊ᭷ពỈ‽ ᮍ‶࡛᭷ព࡜ุᐃࡋࡓࠋ

(24)

16 㸱㸫㸱㸬㸱㸫㸱㸬㸱㸫㸱㸬㸱㸫㸱㸬 ⤖ᯝ⤖ᯝ⤖ᯝ⤖ᯝ 㸱㸫㸱㸫㸯㸬ࣉࣟࣛࢡࢳࣥ 㸱㸫㸱㸫㸯㸬ࣉࣟࣛࢡࢳࣥ 㸱㸫㸱㸫㸯㸬ࣉࣟࣛࢡࢳࣥ 㸱㸫㸱㸫㸯㸬ࣉࣟࣛࢡࢳࣥ )LJ)LJ)LJ)LJ$$$$ ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘࡣࠊዷፎ 㐌࠿ࡽୖ᪼ࢆ♧ࡋࠊዷፎ 㐌࡛ࣆ࣮ࢡ࡟㐩 ࡋࡓࠋࡑࡢᚋࠊ⦆ࡸ࠿࡟ୗ㝆ࡋࠊዷፎ 㐌࡟ࡣࠊᇶᗏ್࡟పୗࡋࡓࠋዷፎ 㐌ࡲ࡛ప್ࢆ⥔ᣢࡋࡓᚋࠊศፔ┤๓ࡢ 㐌࡟ࡣࠊ෌ࡧᛴ⃭࡞ୖ᪼ࢆ♧ࡋࡓࠋ 㸱㸫㸱㸫㸰㸬㸱㸫㸱㸫㸰㸬㸱㸫㸱㸫㸰㸬㸱㸫㸱㸫㸰㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦㯤యᙧᡂ࣍ࣝࣔࣥ㸦㯤యᙧᡂ࣍ࣝࣔࣥ㸦/+㯤యᙧᡂ࣍ࣝࣔࣥ㸦/+/+/+㸧㸧)LJ㸧㸧)LJ)LJ)LJ %%%% ⾑୰ /+ ⃰ᗘࡣࠊዷፎ 㐌࡟ࡣࠊ㧗࠸್ࢆ♧ࡋࠊዷፎ 㐌࡟ࡣᇶᗏ್࡟పୗࡋ ࡓࠋ⾑୰ /+ ⃰ᗘࡣࠊዷፎ 㐌࠿ࡽ෌ࡧᛴ⃭࡞ୖ᪼ࢆ♧ࡋࠊዷፎ 㐌ࢆࣆ࣮ࢡ ࡜ࡋ࡚ࠊࡑࡢᚋᛴ⃭࡞పୗࢆ♧ࡋࡓࠋዷፎ 㐌࡟ࡣᇶᗏ್࡟పୗࡋࠊศፔࡲ࡛ ప್ࢆ⥔ᣢࡋࡓࠋ 㸱㸫㸱㸫㸱㸬㸱㸫㸱㸫㸱㸬㸱㸫㸱㸫㸱㸬

㸱㸫㸱㸫㸱㸬༸⬊่⃭࣍ࣝࣔࣥ㸦༸⬊่⃭࣍ࣝࣔࣥ㸦༸⬊่⃭࣍ࣝࣔࣥ㸦)6+༸⬊่⃭࣍ࣝࣔࣥ㸦)6+)6+)6+㸧㸧)LJ㸧㸧)LJ)LJ)LJ &&&&

⾑୰ )6+ ⃰ᗘࡣࠊዷፎ 㐌࠿ࡽᛴ⃭࡟ୖ᪼ࡋࠊዷፎ 㐌ࢆࣆ࣮ࢡ࡜ࡋ࡚ࠊࡑ ࡢᚋࠊపୗࡋࡓࠋ⾑୰ )6+ ⃰ᗘࡣࠊዷፎ 㐌࠿ࡽዷፎ 㐌ࡲ࡛ప್ࢆ⥔ᣢࡋࠊ ศፔ๓࡟ࡣࠊ⦆ࡸ࠿࡞ୖ᪼ࡀㄆࡵࡽࢀࡓࠋ 㸱㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ 㸱㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ 㸱㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ 㸱㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ )LJ)LJ)LJ)LJ'''' ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡣࠊዷፎ 㐌࠿ࡽୖ᪼ࢆ♧ࡋࠊዷፎ 㐌࡟ࡣ୍ᗘ పୗࡋࡓᚋࠊ෌ᗘዷፎ 㐌࠿ࡽᛴ⃭࡟ୖ᪼ࡋࠊዷፎ 㐌࡟ࣆ࣮ࢡ࡟㐩ࡋࠊࡑࡢ ᚋపୗࡋࡓࠋዷፎ 㐌࠿ࡽዷፎ 㐌ࡲ࡛ࡣࠊQJPO ࡢࣞ࣋ࣝࢆ⥔ᣢࡋࡓࠋዷ ፎ 㐌࡟ࡣࠊ෌ᗘୖ᪼ࢆ♧ࡋࠊศፔᚋ࡟ᛴ⃭࡞పୗࢆ♧ࡋࡓࠋ

(25)

17 㸱㸫㸱㸫㸳㸱㸫㸱㸫㸳㸱㸫㸱㸫㸳㸱㸫㸱㸫㸳㸬ࢸࢫࢺࢫࢸࣟࣥ㸬ࢸࢫࢺࢫࢸࣟࣥ㸬ࢸࢫࢺࢫࢸࣟࣥ㸬ࢸࢫࢺࢫࢸࣟࣥ )LJ)LJ)LJ)LJ(((( ⾑୰ࢸࢫࢺࢫࢸࣟࣥ⃰ᗘࡣࠊࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡢኚ໬࡜Ⰻࡃ୪⾜ࡋࡓኚ ໬ࢆ♧ࡋࡓࠋࡍ࡞ࢃࡕࠊ⾑୰ࢸࢫࢺࢫࢸࣟࣥ⃰ᗘࡣࠊዷፎ 㐌࠿ࡽୖ᪼ࢆ♧ࡋࠊ ዷፎ 㐌࡟ࡣ୍ᗘపୗࡋࡓᚋࠊ෌ᗘ 㐌࠿ࡽᛴ⃭࡞ୖ᪼ࡀㄆࡵࡽࢀࡓࠋࡑࡢᚋࠊ ⦆ࡸ࠿࡞పୗࢆ♧ࡋࠊ෌ࡧዷፎ 㐌࠿ࡽᛴ⃭࡟ୖ᪼ࡋࠊศፔᚋᛴ⃭࡟పୗࡋࡓࠋ 㸱㸫㸱㸫㸴㸱㸫㸱㸫㸴㸱㸫㸱㸫㸴㸱㸫㸱㸫㸴㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ)LJ)LJ)LJ)LJ)))) ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘࡣࠊዷፎ 㐌ࡲ࡛ࡣప್ࢆ♧ࡋࠊዷፎ 㐌࠿ࡽࠊ ᛴ⃭࡞ୖ᪼ࢆ♧ࡋࠊዷፎ 㐌ࡲ࡛㧗್ࢆ⥔ᣢࡋࠊࡑࡢᚋศፔ࡟㏆࡙ࡃ࡟ࡘࢀ࡚ పୗࡋࠊศፔᚋ࡟ᛴ⃭࡟పୗࡋࡓࠋ 㸱㸫㸱㸫㸵㸬࢖ࣥࣄࣅࣥ 㸱㸫㸱㸫㸵㸬࢖ࣥࣄࣅࣥ 㸱㸫㸱㸫㸵㸬࢖ࣥࣄࣅࣥ 㸱㸫㸱㸫㸵㸬࢖ࣥࣄࣅࣥ)LJ)LJ)LJ)LJ**** ⾑୰࢖ࣥࣄࣅࣥ⃰ᗘࡣࠊศፔ᪥࡟ࡣࠊ㧗್ࢆ♧ࡋࠊዷፎ 㹼㐌࡟ࡣࠊ㹼 QJPO ⛬ᗘࡢ⃰ᗘࢆ⥔ᣢࡋࡓᚋࠊ෌ࡧపୗࡋ࡚ዷፎ 㐌࡟ࡣࠊQJPO ௨ ୗ࡟పୗࡋࠊዷፎ 㐌ࡲ࡛ྠࣞ࣋ࣝࢆ⥔ᣢࡋࡓࠋศፔ๓࡟ࡣࠊ෌ࡧ QJPO ࡲ࡛ୖ᪼ࡋࡓࠋ 㸱㸫㸱㸫㸶㸬㸱㸫㸱㸫㸶㸬㸱㸫㸱㸫㸶㸬㸱㸫㸱㸫㸶㸬ዷፎ࣐࢘ࡢ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘ࡜᪥↷᫬㛫ࡢ㛵ಀዷፎ࣐࢘ࡢ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘ࡜᪥↷᫬㛫ࡢ㛵ಀዷፎ࣐࢘ࡢ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘ࡜᪥↷᫬㛫ࡢ㛵ಀ )LJዷፎ࣐࢘ࡢ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘ࡜᪥↷᫬㛫ࡢ㛵ಀ )LJ)LJ)LJ㸧㸧㸧㸧 ዷፎ࣐࢘ࡢ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘࡢኚ໬ࢆࠊ໭ᾏ㐨᪥㧗ᆅ᪉ࡢ᪥↷᫬㛫ࡢኚ໬ 㸦Ẽ㇟ᗇࢹ࣮ࢱ࣮࣋ࢫࢆཧ↷㸧࡜ేࡏ࡚సᡂࡋࡓᅗࢆ )LJ ࡟♧ࡋࡓࠋ⾑୰ ࣉࣟࣛࢡࢳࣥ⃰ᗘࡣࠊ ᭶௨㝆࡟ୖ᪼ࡋࠊ ᭶࡟ࣆ࣮ࢡࢆ♧ࡋࡓᚋ࡟పୗࡋ࡚ ᭶࡟ࡣᇶᗏࣞ࣋ࣝ࡟పୗࡋࡓࠋࡇࡢᇶᗏࣞ࣋ࣝࡣࠊ⩣ᖺࡢ ᭶ࡲ࡛⥔ᣢࡉࢀࡓࠋ ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘࡢኚ໬࡜ᖺ㛫ࡢ᪥↷᫬㛫ࡢኚ໬ࡢ㛫࡟ࡣࠊṇࡢ┦㛵ࡀㄆ ࡵࡽࢀࡓ㸦U S㸧ࠋࡍ࡞ࢃࡕࠊ᪥↷᫬㛫ࡀ㛗࠸᫬ᮇ࡟㧗ࡃࠊ▷ ࠸᫬ᮇ࡟ࡣప࠸್ࢆ♧ࡋࡓ)LJࠋ

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18 㸱㸫㸱㸫㸷㸬㸱㸫㸱㸫㸷㸬㸱㸫㸱㸫㸷㸬㸱㸫㸱㸫㸷㸬ዷፎ࣐࢘ࡢ⾑୰ዷፎ࣐࢘ࡢ⾑୰ዷፎ࣐࢘ࡢ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝዷፎ࣐࢘ࡢ⾑୰࢚ࢫࢺࣛࢪ࣮࢚࢜ࣝࢫࢺࣛࢪ࣮࢚࢜ࣝࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘ࡜⫾Ꮚࡢᛶูࡢ㛵ಀ⃰ᗘ࡜⫾Ꮚࡢᛶูࡢ㛵ಀ⃰ᗘ࡜⫾Ꮚࡢᛶูࡢ㛵ಀ⃰ᗘ࡜⫾Ꮚࡢᛶูࡢ㛵ಀ )LJ)LJ)LJ)LJDDDDE EEE ዷፎᚋ༙࡟ㄆࡵࡽࢀࡓẕ࣐࢘ࡢ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘࢆࠊ㞝⫾Ꮚࢆዷፎ ࡍࡿ࣐࢘࡜㞤⫾Ꮚࢆዷፎࡍࡿ࣐࢘࡟ศࡅ࡚ࠊ⾲♧ࡋࡓࢢࣛࣇࢆ )LJD ࡟♧ࡋ ࡓࠋࡲࡓࠊࡇࡢ஧ࡘࡢࢢࣛࣇࡢ᭤⥺ୗ㠃✚㸦$UHD8QGHUWKH&XUYH㸧ࢆ )LJE ࡟♧ࡋࡓࠋዷፎ 㐌࠿ 㐌ࡲ࡛ࡢࠊዷፎ࣐࢘ࡢ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘࢆ ẚ㍑ࡍࡿ࡜ࠊ㞝⫾Ꮚዷፎ㤿ࡀ㞤⫾Ꮚዷፎ㤿࡟ẚ࡭࡚㧗࠸⃰ᗘࢆ♧ࡋࡓ 㸦)LJD㸧ࠋࡲࡓࠊࡇࡢ㛫ࡢẕ㤿ࡢ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝࡢ $UHD8QGHUWKH &XUYH ࢆẚ㍑ࡍࡿ࡜ࠊ㞝⫾Ꮚዷፎ㤿ࡀ㞤⫾Ꮚዷፎ㤿ࡼࡾࡶ᭷ព࡟㧗࠸್ࢆ♧ࡋࡓ 㸦)LJE㸧ࠋ

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19 㸱㸫㸲㸬⪃ᐹ 㸱㸫㸲㸬⪃ᐹ㸱㸫㸲㸬⪃ᐹ 㸱㸫㸲㸬⪃ᐹ ᮏ◊✲࡛ࡣࠊ᪥ᮏ⏘ࢧࣛࣈࣞࢵࢻ࡟࠾ࡅࡿዷፎ඲ᮇ㛫୰ࡢ ✀㢮ࡢ⏕Ṫ㛵㐃 ࣍ࣝࣔࣥࡢኚ໬ࢆ᫂ࡽ࠿࡟ࡋࡓࠋ㐣ཤࡢ◊✲࡟࠾࠸࡚ࠊ࢚ࢫࢺࣛࢪ࣮࢜ࣝ㸦1HWW HWDO1RGHQHWDO㸧ࠊࣉࣟࢪ࢙ࢫࢸࣟࣥࡸ௚ࡢࣉࣟࢪ࢙ࢫࢳࣥ 㸦1RGHQHWDO6PLWK6TXLUHVHWDO+ROWRQHWDO 6HUHQHWDO㸧ࠊࢸࢫࢺࢫࢸࣟࣥ㸦6LOEHU]DKQHWDO㸧ࡣዷፎ඲ᮇ 㛫ࢆ㏻ࡋ࡚ ᐃࡉࢀ࡚࠸ࡿࠋ༸⬊่⃭࣍ࣝࣔࣥ㸦)6+㸧ࡣࠊዷፎ๓ᮇ㸦(YDQVDQG ,UYLQH0LOOHUHWDO㸧࠾ࡼࡧᚋᮇ㸦1DPERHWDO㸧࡛ࡣ ᐃࡉࢀ࡚࠸ࡿࠋࣉࣟࣛࢡࢳࣥࡣࠊศፔ๓ᚋ㸦3DVKHQ:RUWK\HWDO㸧 ࡛࠶ࡾࠊ࢖ࣥࣄࣅࣥࡣࠊዷፎ๓ᮇ㸦'HUDUHWDO㸧࠾ࡼࡧᚋᮇ㸦1DPERHW DO㸧࡛ࡣ ᐃࡉࢀ࡚࠸ࡿࡀࠊዷፎ඲ᮇ㛫ࢆ㏻ࡋ࡚ ᐃࡉࢀ࡚࠸࡞࠸ࠋᮏ ◊✲ࡣࠊྠ୍㤿ࢆ⏝࠸࡚ዷፎ඲ᮇ㛫ࡢ ✀㢮ࡢ࣍ࣝࣔࣥศἪኚ໬ࢆ᫂ࡽ࠿࡟ࡋ ࡓึࡵ࡚ࡢ◊✲࡛࠶ࡿࠋ ࣉࣟࣛࢡࢳࣥࡣࠊዷፎᮇ㛫୰࡟ࡣࠊ᪥㛗᫬㛫࡜୍⮴ࡋࡓኚ໬ࢆ♧ࡋࡓࠋ༶ࡕࠊ ᪥㛗᫬㛫ࡀ㛗ࡃ࡞ࡿ࡟ࡘࢀ࡚ୖ᪼ࡋࠊ▷ࡃ࡞ࡿ࡟ࡘࢀ࡚పୗࡍࡿᏘ⠇ኚ໬ࡀㄆ ࡵࡽࢀࡓࠋࡇࢀࡽࡢ⤖ᯝ࠿ࡽࠊࣉࣟࣛࢡࢳࣥࡣࠊዷፎ᫬ᮇ࡜ࡣ㛵ಀ࡞ࡃ᪥㛗᫬ 㛫࡟ᙳ㡪ࢆཷࡅࡿ࡜⪃࠼ࡽࢀࡓࠋ୍᪉ࠊᏘ⠇ኚ໬࡜ࡣ↓㛵ಀ࡟ࠊศፔ๓ᚋ࡟ࡣ ᛴ⃭࡞⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘࡢୖ᪼ࡀㄆࡵࡽࢀࡓࠋࡇࢀࡣࠊศፔ࡟క࠺ࢫࢺࣞ ࢫ࡞࡝ࡢ่⃭࡟ࡼࡿ࡜᥎ᐹࡉࢀࡓࠋ᭦࡟ࠊศፔᚋࡣஙᏊ࡟ࡼࡿ྾ங่⃭࡟ࡼࡾ ẕ࣐࢘ࡢࣉࣟࣛࢡࢳࣥศἪࡀ่⃭ࡉࢀࠊ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘࡢୖ᪼ࡀㄆࡵࡽ ࢀࡓࠋ㐣ཤࡢ◊✲࠿ࡽࠊ㠀ዷፎᮇࡢ㞤࣐࡛࢘ࡶྠᵝ࡞ࣉࣟࣛࢡࢳࣥศἪࡢᏘ⠇ ኚ໬ࡀሗ࿌ࡉࢀ࡚࠸ࡿ㸦-RKQVRQ㸧ࠋ ᮏ◊✲࡛ࠊ࣐࢘ዷፎᮇ㛫୰࡟ㄆࡵࡽࢀࡓዷፎ┤ᚋࡢ /+ ࡢ㧗್ࡣࠊ࣐࡛࢘ࡣࠊ ᤼༸ᚋࡶࢧ࣮ࢪࡢᣢ⥆ࡀㄆࡵࡽࢀࡇ࡜࠿ࡽࠊ/+ ࢧ࣮ࢪ࡟ࡼࡿ㧗್࡛࠶ࡿ࡜᥎ᐹ

(28)

20 ࡉࢀࡓࠋዷፎ 㐌㡭࠿ࡽࡢୖ᪼ࡣࠊ/+ ࡢ ᐃ࡟౑⏝ࡋࡓ࣐࢘ /+ ᢠయ࡜஺ᕪᛶࡢ ࠶ࡿ࣐࢘⤧ẟᛶᛶ⭢่⃭࣍ࣝࣔࣥ㸦H&*㸧࡛࠶ࢁ࠺࡜᥎ᐹࡉࢀࡓࠋH&* ࡣࠊ࣍ࣝ ࣔࣥࡢᵓ㐀୰ࡢ⺮ⓑ㉁㒊ศࡀୗᆶయᛶ /+ ࡜ྠ୍࡛࠶ࡿࡇ࡜࠿ࡽࠊ࣐࢘ /+ ᢠయ ࡜ᙉ࠸஺ᕪᛶࢆ♧ࡍࡶࡢ࡛࠶ࡾࠊᮏ◊✲࡛ ᐃࡋࡓ᪉ἲ࡛ࡣࠊዷፎ๓༙ᮇ࡟࠾ ࡅࡿୗᆶయᛶ /+ ࡢṇ☜࡞ศἪኚ໬ࡣ᫂ࡽ࠿࡟࡛ࡁ࡞࠿ࡗࡓࠋዷፎᚋ༙ᮇ࡟ࠊ⾑ ୰ /+ ⃰ᗘࡀᇶᗏ್ࢆ⥔ᣢࡋ⥆ࡅࡓࡢࡣࠊከ㔞࡟ศἪࡉࢀࡿࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ ࡟ࡼࡿ㈇ࡢࣇ࢕࣮ࢻࣂࢵࢡస⏝࡟ࡼࡾどᗋୗ㒊࣭ୗᆶయ࠿ࡽࡢ *Q5+࡜ /+ ศἪ ࡀᙉࡃᢚไࡉࢀࡓ⤖ᯝ࡛࠶ࢁ࠺࡜᥎ᐹࡉࢀࡓࠋ ᮏ◊✲࡟࠾ࡅࡿዷፎ㤿ࡢ⾑୰ )6+ ⃰ᗘࡢኚ໬ࡣࠊH&* ࡜ࣉࣟࣛࢡࢳࣥࡢศἪኚ ໬࡜㢮ఝࡋ࡚࠸ࡿࡓࡵࠊ๪㯤యᙧᡂ࡟⮳ࡿ༸⬊Ⓨ⫱ಁ㐍࡟㛵୚ࡋ࡚࠸ࡿ࡜᥎ᐹ ࡉࢀࡓࠋࡲࡓࠊ)6+ ࡢศἪஹ㐍ࡀ㛗᪥ᮇ࡟ㄆࡵࡽࢀࡿࡇ࡜࠿ࡽࠊᏘ⠇ᛶ࡟ศἪࡉ ࢀ࡚࠸ࡿྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋ㐣ཤࡢ◊✲࡛ࡣࠊ⾑୰ )6+ ⃰ᗘࡀ a ᪥࿘ᮇ ࡛ୖ᪼ࡀㄆࡵࡽࢀ࡚࠸ࡿ㸦(YDQVHWDO0LOOHUHWDO㸧ࡀࠊᮏ◊ ✲࡛ࡣࠊẖ㐌᥇⾑ࡢࡓࡵࠊࡑࡢࡼ࠺࡞ୖ᪼ࡀ㢧ⴭ࡟ㄆࡵࡽࢀ࡞࠿ࡗࡓࠋࡲࡓࠊ ࣐࢘ )6+ ࡣࠊ࣐࢘ /+ ᢠయ࡜஺ᕪࡋ࡞࠸ࡓࡵࠊ ᐃࡉࢀࡓ )6+ ࡣୗᆶయ⏤᮶ࡢෆ ᅉᛶ )6+ ࡛࠶ࢁ࠺࡜᥎ᐹࡉࢀࡓࠋ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘࡀ㧗್ࢆ♧ࡋ࡚࠸ ࡿዷፎᚋ༙ᮇ࡜⾑୰ )6+ ⃰ᗘࡀప್ࢆ♧ࡋ࡚࠸ࡿᮇ㛫ࡀ୍⮴ࡍࡿࡓࡵࠊዷፎᚋ ༙ᮇ࡛ࡣࠊ㧗⃰ᗘࡢ࢚ࢫࢺࣛࢪ࣮࢜ࣝ࡟ࡼࡾศἪࡀᢚไࡉࢀ࡚࠸ࡿ࡜ゎ㔘ࡉࢀ ࡓࠋศፔࡀ㏆࡙ࡃ࡜㧗⃰ᗘࡢ࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘࡀపୗࡋ࡚ࠊ෌ᗘศፔ๓࡟ ⾑୰ )6+ ⃰ᗘࡀୖ᪼ࢆ♧ࡍࡢࡣࠊศፔᚋ᤼༸࡟ྥࡅ࡚༸⬊Ⓨ⫱ࢆಁ㐍ࡋ࡚࠸ࡿ ࡜᥎ᐹࡉࢀࡓࠋ ዷፎ๓༙ᮇ࡟࠾ࡅࡿ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡢ ᅇ┠ࡢୖ᪼࡜ ᅇ┠ࡢୖ ᪼ࡢศἪ※ࡣࠊࡑࢀࡒࢀ᤼༸ࡋࡓ㯤య࡜๪㯤య࡜᥎ᐹࡉࢀࡓࠋዷፎᚋ༙ᮇ࡟ࡣࠊ ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡀపୗࢆ♧ࡍࡀࠊ㠀ዷፎᮇࡼࡾࡶ㧗࠸್ࢆ⥔ᣢࡋࡓࠋ ศፔ 㐌๓࡟ࡣࠊ෌ࡧ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡀୖ᪼ࢆ♧ࡍࡀࠊศፔᚋ࡟ᛴ

(29)

21 ⃭࡞పୗࢆక࠺ࡇ࡜࠿ࡽࠊࡇࡢ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡢศἪ※ࡣࠊ⫾Ꮚ⫾ ┙ࣘࢽࢵࢺ⏤᮶࡛࠶ࡿ࡜ゎ㔘ࡉࢀࡓࠋ+ROWDQ ࡽࡸ 6HUHQ ࡽ࡟ࡼࡿ ࡜ࠊዷፎᮇ๓༙ᮇࡣࠊ୺࡟ SURJHVWHURQH ࡜ ȘK\GUR[\SURJHVWHURQH ࡀศἪ ࡉࢀࠊዷፎᮇᚋ༙ᮇ࡛ࡣࠊ୺࡟ ȘSUHJQDQH ࡀศἪࡉࢀ࡚࠸ࡿ࡜ሗ࿌ࡉࢀ࡚࠸ ࡿࠋᮏ◊✲࡛ࠊዷፎᚋ༙ᮇ࡟ ᐃࡉࢀࡓࣉࣟࢪ࢙ࢫࢸࣟࣥࡣࠊ ᐃ࡟౑⏝ࡋࡓ ࣉࣟࢪ࢙ࢫࢸࣟࣥᢠయ࡟ ȘSUHJQDQH ࡀ⤖ྜࡋࡓྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋ ᮏ◊✲࡟࠾࠸࡚ࠊዷፎ඲ᮇ㛫ࢆ㏻ࡋ࡚⾑୰ࢸࢫࢺࢫࢸࣟࣥ࡜ࣉࣟࢪ࢙ࢫࢸࣟ ࣥ⃰ᗘࡣࠊᖹ⾜ࡋࡓኚ໬ࢆ♧ࡋࡓࡇ࡜࠿ࡽࠊศἪ※ࡣྠ୍࡛࠶ࢁ࠺࡜᥎ᐹࡉࢀ ࡓࠋ༶ࡕࠊึࡵࡢୖ᪼ࡣ᤼༸ࡋࡓ㯤య࠿ࡽࠊ ᅇ┠ࡢୖ᪼ࡣ๪㯤య࠿ࡽࠊศፔ๓ ࡢୖ᪼ࡣ⫾Ꮚ⫾┙ࣘࢽࢵࢺ࡛࠶ࢁ࠺࡜᥎ᐹࡉࢀࡓࠋࣉࣟࢪ࢙ࢫࢸࣟࣥࡣࢸࢫࢺ ࢫࢸࣟࣥࡢᇶ㉁࡛࠶ࡾࠊ㓝⣲࡟ࡼࡗ࡚ࢸࢫࢺࢫࢸࣟࣥ࡟ኚ᥮ࡉࢀࡿࠋዷፎ୰࡟ ࡣࠊ㠀ዷፎᮇࡢ⾑୰⃰ᗘࡼࡾࡶ㢧ⴭ࡞ୖ᪼ࡀ㉳ࡇࡿࡓࡵࠊ༸ᕢࡸ⫾┙࡛ࣉࣟࢪ ࢙ࢫࢸࣟࣥࡀࢸࢫࢺࢫࢸࣟࣥ࡟ኚ᥮ࡉࢀࠊ㢮ఝࡋࡓศἪኚ໬ࢆ♧ࡋࡓ࡜᥎ᐹࡉ ࢀࡓࠋ6LOEHU]DKQ ࡽ㸦㸧࡟ࡼࡿ࡜ࠊ⾑୰ࢸࢫࢺࢫࢸࣟࣥ⃰ᗘࡣዷፎᚋ࡟ᛴ ⃭࡞ୖ᪼ࡀㄆࡵࡽࢀࡿࡢࡣࠊ௒ᅇࡢ⤖ᯝ࡜୍⮴ࡍࡿࠋࡋ࠿ࡋࠊࡑࡢᚋࠊୖ᪼ࢆ ♧ࡋࠊ⣙ ᪥࡟ࣆ࣮ࢡ࡟㐩ࡋ࡚࠸ࡿࡓࡵࠊ௒ᅇࡢ⤖ᯝ࡜୍⮴ࡋ࡞࠿ࡗࡓࠋ ᐃ࡟౑⏝ࡋࡓࢸࢫࢺࢫࢸࣟࣥᢠయࡢ஺ᕪ཯ᛂᛶ࡞࡝ࡢ㐪࠸࡞࡝ࡀᙳ㡪ࡋ࡚࠸ࡿ ࡜᥎ᐹࡉࢀࡿࠋ ࣐࢘⫾Ꮚᛶ⭢ࡣࠊዷፎ୰ᮇ࠿ࡽᚋᮇ࡟࠿ࡅ࡚ࠊ⫧኱໬ࡍࡿࡀࠊࡑࡢ኱ࡁࡉࡢ ኚ໬࡜ẕ㤿ࡢ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘࡢኚ໬ࡣ୍⮴ࡍࡿ࡜ሗ࿌ࡉࢀ࡚࠸ࡿ 㸦⏣୰㸧ࠋ㞝⫾Ꮚዷፎ㤿࡜㞤⫾Ꮚዷፎ㤿ࡢẕ㤿ࡢ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ ᗘࡢ $8& ࢆẚ㍑ࡍࡿ࡜ࠊ㞝⫾Ꮚዷፎ㤿ࡢ࡯࠺ࡀ᭷ព࡟㧗࠸್ࢆ♧ࡋࡓࠋ㞝⫾Ꮚ ᛶ⭢ࡢࢸࢫࢺࢫࢸࣟࣥྵ᭷㔞ࡣࠊ㞤⫾Ꮚᛶ⭢࡜ẚ㍑ࡋ࡚ࠊ㧗࠸࡜ሗ࿌ࡉࢀ࡚࠸ ࡿࡇ࡜࠿ࡽ㸦⏣୰㸧ࠊẕ㤿࡬ࡢ࢚ࢫࢺࣛࢪ࣮࢜ࣝ๓㥑≀㉁ࡢ⛣⾜ࡀ㞤⫾Ꮚ ࡜ẚ㍑ࡋ࡚ࠊ㞝⫾Ꮚࡢ࡯࠺ࡀከ࠸ࡓࡵ࡛࠶ࡿ࡜᥎ᐹࡉࢀࡓࠋ

(30)

22 ᮏ◊✲࡟࠾࠸࡚ࠊ⾑୰࢖ࣥࣄࣅࣥ⃰ᗘࡣࠊዷፎ๓༙ᮇ࡟㧗್ࢆ♧ࡋࡓࠋࡇࡢ ⾑୰࢖ࣥࣄࣅࣥ⃰ᗘࡢศἪ※ࡣࠊዷፎ 㐌㡭ࡲ࡛ࡣ༸ᕢ࡟Ⓨ⫱ࡋࡓ༸⬊࡛࠶ࢁ ࠺࡜᥎ᐹࡉࢀࡓࠋዷፎ୰ᮇ࡟ࡣࠊẕ࣐࢘ࡢ༸ᕢ࡛ࡣ༸⬊Ⓨ⫱ࡀᢚไࡉࢀ࡚࠸ࡿ ࡇ࡜࠿ࡽ࢖ࣥࣄࣅࣥࡢศἪ⬟ࡀపୗࡍࡿ࡜᥎ᐹࡉࢀࡓࠋዷፎ୰ᮇ࠿ࡽᚋᮇ࡟࠿ ࡅ࡚ୖ᪼ࡀㄆࡵࡽࢀࡓ࡜࠸࠺ሗ࿌㸦1DPERHWDO㸧ࡀ࠶ࡿࡀࠊᮏ◊✲࡛ࡣࠊ ୖ᪼ࡀㄆࡵࡽࢀ࡞࠿ࡗࡓࠋࡲࡓࠊศፔ┤๓࡟⾑୰࢖ࣥࣄࣅࣥ⃰ᗘࡢୖ᪼ࡀㄆࡵ ࡽࢀࡓⅬࡣࠊ1DPER ࡽࡢሗ࿌࡟୍⮴ࡋࡓࠋዷፎᮎᮇ࡟ࡣࠊ)6+ ࡢศἪୖ᪼ ࡟ࡼࡾࠊศፔᚋ᤼༸࡟ྥࡅ࡚༸ᕢ࡛༸⬊ࡀⓎ⫱ࡍࡿࡓࡵࠊ⾑୰࢖ࣥࣄࣅࣥ⃰ᗘ ࡀୖ᪼ࡋࡓ⤖ᯝ࡛࠶ࢁ࠺࡜᥎ᐹࡉࢀࡓࠋ

(31)

23 0 10 20 30 0 5 10 15 20 35 40 45 50 C Week FS H (n g /m l) 0 10 20 30 0 5 10 35 40 45 50 500 1000 1000 1500 2000 2500 3000 3500 B We e k LH (n g/ mOᤢOᤢOᤢOᤢ 0 10 20 30 0 1 2 3 4 5 35 40 45 50 10 20 30 A We e k P ro la cti n( ng /m l) 0 10 20 30 0.0 0.5 1.0 1.5 2.0 2.5 35 40 45 50

G

We ek ir -i n h ib in (n g /m l) 0 10 20 30 0 10 20 30 35 40 45 50 D Wee k P ro g e s te ro n e (n g /m l) 0 10 20 30 0 200 400 600 35 40 45 50 F Wee k Es tr ad io l-17ββββ (p g /m l) 0 10 20 30 0 50 100 150 200 250 35 40 45 50 We e k Te s tos te rone (pg/ m l) E

Fig.3-1 Change of plasma concentration of Prolactin(A),LH(B),FSH(C),

Progesterone(D), Testosterone (E) Estradiol-17β(F),ir-inhibin(G) during pregnancy. Results are expressed as means ± SEM.

(32)

24

0

2

4

6

0

10

20 P

R

L(

ng/

m

l)

D

ay

Le

ngth

(h

)

M A M J J A S O N D J F M A

Month

Fig.3-2 Comparing the change of PRL concentration with the day length (h).

Left (●) shows the change of PRL concentration and results are expressed as means ± SEM. Right (line) shows day length.

Coefficient of correlation with the change of PRL concentration and day length is 0.8003 (p< 0.0001).

(33)

25 0 10 20 30 40 50 0 200 400 600 male female Week

(

VW

UDGL

RO

(

VW

UDGL

RO

(

VW

UDGL

RO

(

VW

UDGL

RO

ββββ

ᤡSJ

PO

ᤢ

ᤡSJ

PO

ᤢ

ᤡSJ

PO

ᤢ

ᤡSJ

PO

ᤢ

(a) ma le fem ale 0 5000 10000 15000 A rea U n d e r t h e C u rv e (A U C ) (b) _male female

*

Fig.3-3 (a)Changes in plasma concentration of maternal estradiol-17,

devided by carried fetal sex. Results are expressed as means ± SEM (b)Comparison of AUC(Area Under the Curve) for upper graph from 24 week

(34)

26 㸲㸲㸲㸲❶❶❶ ศፔᚋึᅇⓎ᝟ࡢෆศἪᏛⓗኚ໬❶ ศፔᚋึᅇⓎ᝟ࡢෆศἪᏛⓗኚ໬ศፔᚋึᅇⓎ᝟ࡢෆศἪᏛⓗኚ໬ศፔᚋึᅇⓎ᝟ࡢෆศἪᏛⓗኚ໬ 㸲㸲㸲㸲㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ㸫㸯㸬ᐇ㦂ࡢ⫼ᬒ ❶࡛ࡣࠊ࣐࢘ࡢዷፎᮇ࡟࠾ࡅࡿෆศἪᏛⓗኚ໬࡟ࡘ࠸࡚᫂ࡽ࠿࡟ࡋࡓࠋ࣐࢘ ࡢ⦾ṪᏛⓗ࡞኱ࡁ࡞≉ᚩ࡜ࡋ࡚ࠊࠕIRDOKHDWࠖࡀᣲࡆࡽࢀࡿࡀࠊࡑࡢෆศἪᏛ ⓗ◊✲ࡢሗ࿌ࡣᑡ࡞࠸ࠋ 㞤࣐࡛࢘ࡣࠊศፔᚋ 㹼 ᪥࡜࠸࠺ᴟࡵ࡚᪩࠸᫬ᮇ࡟ศፔᚋึᅇⓎ᝟ࡀᅇᖐࡋࠊ ≉࡟ IRDOKHDW ࡜࿧ࡤࢀࠊศፔᚋ ᪥๓ᚋ࡛᤼༸ࡋࠊཷ⫾ྍ⬟࡜࡞ࡿ㸦*LQWKHU 㸧ࠋ ⌧ᅾࠊ᪥ᮏ࡟࠾ࡅࡿ࣐࢘ࡢ⏝㏵ࡣࠊ᪥ᮏ࡛㣫㣴ࡉࢀ࡚࠸ࡿ࣐࢘ࡢ⣙ 㸣ࢆ➇ ㉮㤿ࡀ༨ࡵ࡚࠸ࡿࠋ➇㉮㤿⏝࡜ࡋ࡚ࠊ⌧ᅾࢧࣛࣈࣞࢵࢻࡀ⣙ 㢌⏕⏘ࡉࢀ ࡚࠸ࡿࠋ 㞤㤿ࡢዷፎᮇ㛫ࡣࠊ⣙ ᪥࡛࠶ࡾࠊⓎ᝟࿘ᮇࡀ⣙ ᪥࡛࠶ࡿࡢ࡛ࠊ ᖺ ⏘ࢧ࢖ࢡࣝࢆ⥔ᣢࡍࡿࡓࡵ࡟ࡣึᅇⓎ᝟᤼༸࡛ࡢཷ⫾ࡀᮃࡲࢀ࡚࠸ࡿࠋ ࡋ࠿ࡋࠊศፔᚋึᅇⓎ᝟࡟࠾ࡅࡿཷ⫾⋡ࡣ 㹼㸣࡜పࡃ㸦ᚋᲄ 5REHUWV㸧ࠊḟᅇⓎ᝟࡟࠾ࡅࡿཷ⫾⋡ࡢ࡯࠺ࡀ㧗࠸࡜ࡢሗ࿌ࡀ࠶ࡿࠋࡑࡢ ࡼ࠺࡞≧ἣ࡟ࡼࡾࠊศፔᚋึᅇⓎ᝟᫬࡛ࡣ஺㓄ࡏࡎࠊḟᅇⓎ᝟᫬࡛ࡢ஺㓄ࢆ᥎ ዡࡍࡿពぢࡶከ࠸ࠋᐇ㝿ࡣࠊ⌧ᅾࡢ᪥ᮏࡢ➇㉮㤿⏕⏘ࡢ⌧ሙ࡛ࡣࠊከࡃࡢ⦾Ṫ 㞤㤿࡛ศፔᚋึᅇⓎ᝟᫬࡟஺㓄ࡀ⾜ࢃࢀ࡚࠸ࡿࠋ ᮏ◊✲࡛ࡣࠊࢧࣛࣈࣞࢵࢻ✀ࡢ㞤㤿ࡢฟ⏘ᚋ࠿ࡽศፔᚋⓎ᝟࣭᤼༸࡟⮳ࡿࡲ ࡛ࡢ⏕ṪෆศἪᏛⓗኚ໬ࢆ᫂ࡽ࠿࡟ࡍࡿࡇ࡜ࢆ┠ⓗ࡜ࡋࡓࠋ

(35)

27 㸲㸲㸲㸲㸫㸰㸬ᮦᩱ࠾ࡼࡧ᪉ἲ㸫㸰㸬ᮦᩱ࠾ࡼࡧ᪉ἲ㸫㸰㸬ᮦᩱ࠾ࡼࡧ᪉ἲ㸫㸰㸬ᮦᩱ࠾ࡼࡧ᪉ἲ 㸲㸲㸲㸲㸫㸰㸫㸯㸬౪ヨ㤿㸫㸰㸫㸯㸬౪ヨ㤿㸫㸰㸫㸯㸬౪ヨ㤿㸫㸰㸫㸯㸬౪ヨ㤿 ໭ᾏ㐨ⓑ⪁⏫࡟࡚⦾Ṫ⏝࡟㣫㣴ࡉࢀ࡚࠸ࡓࢧࣛࣈࣞࢵࢻ✀ࡢ㞤㤿ࡢ࠺ࡕࠊ ᖺ ᭶ ᪥࠿ࡽ ᭶ ᪥࡟ศፔࡋࡓ 㢌ࢆ⏝࠸ࡓࠋ㞤㤿ࡢᖹᆒᖺ㱋ࡣࠊ s㸦㹼㸧ṓࠊ⏘ḟᩘࡣ s㸦㹼㸧⏘ࠊᅾ⫾᪥ᩘࡣ s㸦㹼㸧᪥࡛࠶ࡗࡓࠋ౪ヨ㤿ࡣ ᭶୰᪪࠿ࡽ ᭶ୗ᪪ࡲ࡛ࡢᮇ㛫ࠊ᪩ᮅ ᫬༙ ࠿ࡽኪ ᫬༙ࡲ࡛ࡢ 㹼 ᫬㛫ࡢ᪥㛗᫬㛫࡟࡞ࡿࡼ࠺࡟⺯ගⅉࢆ⏝࠸࡚ග⥺ฎ ⌮ࡉࢀࡓࠋศፔ๓ᚋࢆ㏻ࡋ࡚ᇶᮏⓗ࡟ඹ㏻ࡋࡓ⤥㣵ෆᐜ࡛࠶ࡗࡓࠋ ࡞࠾ࠊึᅇ᤼༸࡛ཷ⫾ࡋࡓಶయࡣ 㢌ࠊ୙ཷ⫾ࡢಶయࡣ 㢌࡛࠶ࡗࡓࠋ 㸲㸲㸲㸲㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮㸫㸰㸫㸰㸬⾑ᾮࡢ᥇ྲྀ࠾ࡼࡧฎ⌮ ᥇⾑ࡣࠊ㢕㟼⬦࠿ࡽࠊ㞤㤿ࡢศፔ᫬㸦 ᪥㸧࠾ࡼࡧศፔᚋ 㹼 ᪥ࡲ࡛ࡢẖ᪥ࠊ ศፔᚋ ᪥࠿ࡽ ᪥ࡲ࡛ࡢ㝸᪥ࡢィ ᅇ⾜ࡗࡓࠋ㐲ᚰศ㞳࡟ࡼࡾ⾑Ύࢆศ㞳 ࡋ࡚࣍ࣝࣔࣥࡢ ᐃࡲ࡛㸫Υ࡛෭෾ಖᏑࡋࡓࠋ 㸲㸲㸲㸲㸫㸰㸫㸱㸬㉸㡢Ἴ⏬ീデ᩿࡟ࡼࡿศፔᚋࡢ༸ᕢࡢほᐹ㸫㸰㸫㸱㸬㉸㡢Ἴ⏬ീデ᩿࡟ࡼࡿศፔᚋࡢ༸ᕢࡢほᐹ㸫㸰㸫㸱㸬㉸㡢Ἴ⏬ീデ᩿࡟ࡼࡿศፔᚋࡢ༸ᕢࡢほᐹ㸫㸰㸫㸱㸬㉸㡢Ἴ⏬ീデ᩿࡟ࡼࡿศፔᚋࡢ༸ᕢࡢほᐹ ศፔᚋ ᪥ᚋ௨㝆ࡢ༸ᕢࡢᵝᏊࢆࠊ㉸㡢Ἴ⏬ീデ᩿⿦⨨㸦(&+2&$0(5$66%ࠊ $/2.$ࠊᮾி㸧ࢆ⏝࠸ࠊ┤⭠ࢆ௓ࡋ࡚ẖ᪥ほᐹࡋࡓࠋ๓᪥ࡢᵝᏊ࡜ẚ㍑ࡋࠊ༸⬊ ࡜⪃࠼ࡽࢀࡿീࡀ᫂ࡽ࠿࡟ᾘኻࡋࡓ᪥ࢆ᤼༸☜ㄆ᪥࡜ࡋࡓࠋ 㸲㸲㸲㸲㸫㸰㸫㸲㸬㸫㸰㸫㸲㸬㸫㸰㸫㸲㸬㸫㸰㸫㸲㸬ྛ✀ྛ✀ྛ✀࣍ࣝࣔࣥྛ✀࣍ࣝࣔࣥ࣍ࣝࣔࣥࡢ࣍ࣝࣔࣥࡢࡢࡢ ᐃ ᐃ ᐃ ᐃ 㸲㸲㸲㸲㸫㸰㸫㸲㸫㸯㸬ࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖ἲ㸫㸰㸫㸲㸫㸯㸬ࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖ἲ㸫㸰㸫㸲㸫㸯㸬ࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖ἲ㸫㸰㸫㸲㸫㸯㸬ࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖ἲ ⾑୰ /+ࠊ)6+ࠊࣉࣟࣛࢡࢳࣥࠊ࢚ࢫࢺࣛࢪ࣮࢜ࣝșࠊࢸࢫࢺࢫࢸࣟࣥ⃰ᗘ

(36)

28 ࢆࠊ➨ ❶࡟グ㏙ࡋࡓࣛࢪ࢜࢖࣒ࣀ࢔ࢵࢭ࢖ἲ࡟࡚ ᐃࡋࡓࠋ 㸲㸲㸲㸲㸫㸰㸫㸲㸫㸰㸬㓝⣲ච␿ ᐃἲ㸦㸫㸰㸫㸲㸫㸰㸬㓝⣲ච␿ ᐃἲ㸦㸫㸰㸫㸲㸫㸰㸬㓝⣲ච␿ ᐃἲ㸦㸫㸰㸫㸲㸫㸰㸬㓝⣲ච␿ ᐃἲ㸦(,$(,$(,$(,$㸧㸧㸧㸧 3UDNDVK ࡽࡢ᪉ἲࢆᨵⰋࡋࡓ ᢠయ㓝⣲ᢠయච␿ ᐃἲ㸦(,$㸧࡟ࡼࡗ࡚ࠊ⾑ ୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡢ ᐃࢆ⾜ࡗࡓࠋ ࣍ࣝࣔࣥᢳฟࡣࠊࢪ࢚ࢳ࢚࣮ࣝࢸࣝ࡟ࡼࡿ ᅇᢳฟἲࢆ⏝࠸ࡓࠋ ࡲࡓࡣ ȣO ࡢ⾑Ύ࡟㉸⣧Ỉࢆຍ࠼࡚ ȣO ࡟ࡋࡓࡶࡢࢆࠊPP ࡢ࣓ࢱࣀ࣮ ࣝὙί῭ࡳ࢞ࣛࢫヨ㦂⟶࡟ศὀࡋࡓࠋ⾑Ύࢧࣥࣉࣝ࡟ࢪ࢚ࢳ࢚࣮ࣝࢸࣝ 㹼 PO ࢆຍ࠼ࠊ ศ᧠ᢾࡉࡏࡓࠋ⣙ ศ㛫㟼⨨ᚋࠊ࣓ࢱࣀ࣮ࣝࢆ෭፹࡜ࡋࡓࢡ ࣛ࢖࢜ࢡ࣮ࣝ㸦(<(/$ࠊᮾிࠊ᪥ᮏ㸧࡟ᾐࡋࠊỈᒙࢆ෾⤖ࡉࡏࠊ࢚࣮ࢸࣝᒙࢆ ᐃ⏝ヨ㦂⟶㸦PP㸧࡟⛣ࡋࡓࠋḟ࠸࡛ Υࡢᜏ ᵴ࡟࡚ࢪ࢚ࢳ࢚࣮ࣝࢸࣝ ࢆ஝ᅛᚋࠊPO ࡢࢪ࢚ࢳ࢚࣮ࣝࢸࣝࢆຍ࠼ࠊ෌஝ᅛࡋࡓࠋ&D_{࠾ࡼࡧ 0J}_ࢆྵ ᭷ࡋ࡚࠸࡞࠸ 0'XOEHFFR̓V3KRVSKDWH%XIIHUHG6DOLQH㸦'3%6㸧࡟ %RYLQH6HUXP$OEPLQ㸦$ࠊ6,*0$ࠊ6W/RXLVࠊ0LVVRXULࠊ86$㸧ࢆ ⁐ゎࡉࡏࡓᾮࢆ࢔ࢵࢭ࢖⦆⾪ᾮ࡜ࡋࠊࡇࢀࢆྛヨ㦂⟶࡟ ȣO ศὀࡋࠊ ศ㛫 ᧠ᢾࡋࡓᚋࠊ ᐃ࡟⏝࠸ࡓࠋ ᐃ⏝ࣉ࣮ࣞࢺ࡜ࡋ࡚ࠊZHOO ࢔ࢵࢭ࢖ࣉ࣮ࣞࢺ㸦(/,6$SODWH&RVWDUࠊ &RUQLQJࠊ1HZ<RUNࠊ86$㸧ࢆ⏝࠸ࡓࠋ➨ ᢠయ࡜ࡋ࡚ࠊࣖࢠᢠ࢘ࢧࢠ ,J* ⾑ Ύ㸦$U**ࠊ1Rࠊ-DFNVRQࠊ3HQQV\OYDQLDࠊ86$㸧ࢆ⏝࠸ࠊࡇࢀࢆ ࢥ࣮ࢸ࢕ࣥࢢ⏝⦆⾪ᾮ㸦0&DUERQDWH%XIIHU㸧࡛ ȣJPO ࡟ᕼ㔘ࡋࠊ࢔ࢵ ࢭ࢖ࣉ࣮ࣞࢺ࡟ ȣOZHOO ຍ࠼ࡓࠋᐊ ࡛ ᫬㛫㟼⨨ᚋࠊ࢙࢘ࣝෆࡢ $U** ࢆᤞ࡚ࠊࣈࣟࢵ࢟ࣥࢢ⏝⦆⾪ᾮ㸦*HODWLQ6XFURVH 7KLPHURVRO'3%6㸧ࢆ ȣOZHOO ຍ࠼ࠊ ᐃ࡟⏝࠸ࡿࡲ࡛㐽ගࡋ Υ࡛ಖᏑ ࡋࡓࠋ ᐃ⏝ࣉ࣮ࣞࢺࢆ (,$ Ὑί⦆⾪ᾮ㸦7ZHHQຍ'3%6㸧࡛ ᅇὙί

(37)

29 ࡋࠊࣉࣟࢪ࢙ࢫࢸࣟࣥᶆ‽≀㉁㸦3ࠊ6,*0$ࠊ6W/RXLVࠊ0LVVRXULࠊ86$㸧 ࢆ (,$ ࢔ࢵࢭ࢖⦆⾪ᾮ࡛ ಸẁ㝵ᕼ㔘ࡋࡓ ✀㢮ࡢ⃰ᗘࡢᶆ‽⁐ᾮࠊࡲࡓࡣᢳ ฟࢧࣥࣉࣝࢆ ȣOZHOO ศὀࡋࡓᚋࠊ➨ ᢠయ࡜ࡋ࡚ࠊ࢘ࢧࢠᢠࣉࣟࢪ࢙ࢫࢸ ࣟࣥᢠయ㸦ࠊ%LRJHQHVLVࠊ:HVWPLQVWHU&RORUDGR86$㸧ࢆ (,$ ࢔ࢵ ࢭ࢖⦆⾪ᾮ࡛ ಸ࡟ᕼ㔘ࡋ࡚ ȣOZHOO ศὀࡋࠊᐊ ࡛ ᫬㛫࢖ࣥ࢟ࣗ࣋ ࣮ࢺࡋࡓࠋᶆ‽≀㉁࡜ࡋ࡚ࠊ3URJHVWHURQH2FDUER[\PHWK\O2[LPH㸦3ࠊ 6,*0$ࠊ6W/RXLVࠊ0LVVRXULࠊ86$㸧ࢆ࣮࣍ࢫࣛࢹ࢕ࢵࢩࣗ࣌ࣝ࢜࢟ࢩࢲ࣮ ࢮ㸦+53ࠊ3ࠊ6,*0$ࠊ6W/RXLVࠊ0LVVRXULࠊ86$㸧࡛ᶆ㆑ࡋࡓ +53 ᶆ㆑ ࣉࣟࢪ࢙ࢫࢸࣟࣥࢆ⏝࠸ࠊࡇࢀࢆ (,$ ࢔ࢵࢭ࢖⦆⾪ᾮ࡛ ಸ࡟ᕼ㔘ࡋࠊࡉ ࡽ࡟ ȣOZHOO ศὀᚋࠊᐊ ࡛ ᫬㛫࢖࣮ࣥ࢟ࣗ࣋ࢺࡋࡓࠋ ᐃ⏝ࣉ࣮ࣞࢺ ࢆ (,$ Ὑί⦆⾪ᾮ࡛ ᅇὙίࡋࠊ70%㸦̓̓7HWUDPHWK\OEHQ]LQHࠊ 5RFKHࠊ*HUPDQ\㸧࡜ '062㸦'LPHWK\O6XOIR[LGHࠊ.$172&KHPLFDOࠊᮾி㸧ࢆᇶ ㉁ᕼ㔘ᾮ㸦+20&LWULF$FLG032㸧࡟⁐ゎࡋࡓᇶ㉁ᾮࢆ ȣOZHOO ศὀࡋࡓࠋࡇࢀࢆᐊ ࡛ ศ࠿ࡽ ᫬㛫཯ᛂࡉࡏࡓᚋࠊ཯ᛂ೵Ṇᾮ㸦 0+62㸧ࢆ ȣOZHOO ຍ࠼࡚཯ᛂࢆ೵Ṇࡉࡏࠊࢥࣟࢼ࣐࢖ࢡࣟࣉ࣮ࣞࢺ࣮ࣜࢲ ࣮㸦073ࠊࢥࣟࢼ㟁Ẽࠊᮾி㸧࡟ࡼࡾ QP ࡢἼ㛗࡛྾ගᗘࢆ ᐃࡋࡓࠋ 㸲㸲㸲㸲㸫㸰㸫㸳㸬⤫ィฎ⌮㸫㸰㸫㸳㸬⤫ィฎ⌮㸫㸰㸫㸳㸬⤫ィฎ⌮㸫㸰㸫㸳㸬⤫ィฎ⌮ ࡍ࡭࡚ࡢ⤖ᯝࡣࠊᖹᆒsᶆ‽ㄗᕪ࡛♧ࡍࠋ ᤼༸᪥ࢆᖹᆒࡢ㸯㸯᪥࡟タᐃࡋࠊࢢࣛࣇࢆసᡂࡋࡓࠋ⾑୰ྛ✀࣍ࣝࣔࣥ⃰ᗘ ࡟ࡘ࠸࡚ࡣࠊ ඖ㓄⨨ࡢศᩓศᯒἲࢆ⏝࠸࡚ࠊኚືࡢ᭷ពᛶࢆ᳨ᐃࡋࡓࠋࡲࡓࠊ ཷ⫾㤿࡜୙ཷ⫾㤿ࡢ୧⩌㛫ࡢ࣍ࣝࣔࣥ⃰ᗘࢆ ඖ㓄⨨ࡢศᩓศᯒཬࡧ 3RVW+RF ࢸࢫࢺࢆ⏝࠸࡚ẚ㍑ࡋࠊ᭷ពᕪࢆ᳨ウࡋࡓࠋ ඲࡚ࡢ⤫ィฎ⌮࡟㛵ࡋ࡚ࠊ᭷ពỈ‽ ᮍ‶࡛᭷ព࡜ุᐃࡋࡓࠋ

(38)

30 㸲㸲㸲㸲㸫㸱㸬⤖ᯝ㸫㸱㸬⤖ᯝ㸫㸱㸬⤖ᯝ㸫㸱㸬⤖ᯝ 㸲㸲㸲㸲㸫㸱㸫㸯㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦㸫㸱㸫㸯㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦㸫㸱㸫㸯㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦㸫㸱㸫㸯㸬㯤యᙧᡂ࣍ࣝࣔࣥ㸦/+/+/+/+㸧㸧㸧㸧)LJ)LJ)LJ)LJ ⾑୰ /+ ⃰ᗘࡣࠊศፔᚋ⦆ࡸ࠿࡟ୖ᪼ࡋࡓᚋࠊศፔᚋ ᪥࠿ࡽᛴ⃭࡟ୖ᪼ࡋࠊ ศፔᚋ ᪥࡟ࣆ࣮ࢡ࡟㐩ࡋࡓᚋపୗࡋࡓࠋศፔᚋ ᪥࡟ࡣࠊศፔ᪥࡜ᕪࡢ࡞࠸ ᇶᗏࣞ࣋ࣝ࡟ࡲ࡛పୗࡋࡓࠋྛಶయࡢ /+ ࣆ࣮ࢡฟ⌧᪥ࡣࠊศፔᚋ s ᪥ ࡛࠶ࡗࡓࠋ⾑୰ /+ ⃰ᗘࡣࠊ᤼༸ ᪥๓࡟ࣆ࣮ࢡ್ࢆ♧ࡋࠊ᤼༸ᚋ ᪥┠࡟ࡣᇶ ᗏ್࡟ࡲ࡛పୗࡋࡓࠋཷ⫾ࡋࡓ㤿ࡣࠊศፔᚋ ᪥ࡲ࡛ᇶᗏ್ࢆ⥔ᣢࡋࡓࡀࠊ୙ ཷ⫾㤿࡛ࡣࠊศፔᚋ ᪥㸦᤼༸ᚋ ᪥㸧࠿ࡽ෌ࡧୖ᪼ࢆ♧ࡋࡓࠋ᤼༸ᚋ ᪥ 㛫ࡢ⾑୰ /+ ⃰ᗘࡣࠊཷ⫾㤿࡜୙ཷ⫾㤿࡟ᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋ 㸲㸲㸲㸲㸫㸱㸫㸰㸬༸⬊่⃭࣍ࣝࣔࣥ㸦㸫㸱㸫㸰㸬༸⬊่⃭࣍ࣝࣔࣥ㸦㸫㸱㸫㸰㸬༸⬊่⃭࣍ࣝࣔࣥ㸦㸫㸱㸫㸰㸬༸⬊่⃭࣍ࣝࣔࣥ㸦)6+)6+)6+)6+㸧㸧㸧)LJ㸧)LJ)LJ)LJ ⾑୰ )6+ ⃰ᗘࡣࠊศፔᚋ㏿ࡸ࠿࡟పୗࡋࠊศፔᚋ ᪥࡟ࡣࠊᇶᗏ್࡟ࡲ࡛ప ୗࡋࡓࠋศፔᚋ ᪥ࡲ࡛ࡣࠊప್ࢆ⥔ᣢࡋࠊศፔᚋ ᪥㸦᤼༸ ᪥๓㸧࡟ᛴ⃭ ࡟ୖ᪼ࡋ୍࡚㐣ᛶ࡟ࣆ࣮ࢡ࡟㐩ࡋࡓᚋࠊศፔᚋ ᪥ࡲ࡛పୗࡋ࡚ࠊࡑࡢᚋศፔ ᚋ ᪥ࡲ࡛ྠࣞ࣋ࣝࢆ⥔ᣢࡋࡓࠋ᤼༸ࡣࠊศፔᚋ ᪥࡟ㄆࡵࡽࢀࡓࠋཷ⫾㤿 ࡜୙ཷ⫾㤿࡛ࠊ⾑୰ )6+ ⃰ᗘࡢศἪኚ໬࡟ᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋ 㸲㸲㸲㸲㸫㸱㸫㸱㸬ࣉࣟࣛࢡࢳࣥ㸫㸱㸫㸱㸬ࣉࣟࣛࢡࢳࣥ㸫㸱㸫㸱㸬ࣉࣟࣛࢡࢳࣥ㸫㸱㸫㸱㸬ࣉࣟࣛࢡࢳࣥ)LJ)LJ)LJ)LJ ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘࡣࠊศፔᙜ᪥ࡣ㧗್࡛࠶ࡗࡓࡀࠊศፔᚋ ᪥ࡲ࡛ᛴ⃭ ࡟పୗࡋࠊศፔᚋ ᪥ࡲ࡛పୗࢆ⥆ࡅࡓࠋࡑࡢᚋศፔᚋ ᪥ࡲ࡛ྠࣞ࣋ࣝࢆ⥔ ᣢࡋࡓᚋࠊศፔᚋ ᪥㸦᤼༸ᚋ ᪥㸧࡟෌ࡧపୗࡋࠊศፔᚋ ᪥௨㝆࡟ኚ໬ ࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋཷ⫾㤿࡜୙ཷ⫾㤿࡛ࠊ⾑୰ࣉࣟࣛࢡࢳࣥ⃰ᗘࡢศἪኚ໬ ࡟ᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋ

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31 㸲㸲㸲㸲㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ㸫㸱㸫㸲㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ)LJ)LJ)LJ)LJ ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡣࠊศፔᚋ㏿ࡸ࠿࡟పୗࡋࠊศፔᚋ ᪥௨㝆᤼༸ ๓᪥ࡢศፔᚋ ᪥ࡲ࡛ᇶᗏ್ࢆ⥔ᣢࡋࡓࠋ᤼༸ᚋࡣࠊᛴ⃭࡟ୖ᪼ࡋࠊཷ⫾㤿࡜ ୙ཷ⫾㤿࡛ࠊ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡢኚ໬࡟㐪࠸ࡀㄆࡵࡽࢀࡓࠋ୙ཷ⫾㤿 ࡛ࡣࠊศፔᚋ ᪥ࠊཷ⫾㤿࡛ࡣศፔᚋ ᪥࡟ࣆ࣮ࢡ࡟㐩ࡋࡓࠋ୙ཷ⫾㤿࡛ࡣࠊ ศፔᚋ ᪥㸦᤼༸ᚋ ᪥㸧ࡲ࡛㧗್ࢆ⥔ᣢࡋࡓᚋపୗࡋࠊศፔᚋ ᪥࡟ࡣᇶ ᗏ್࡟ࡲ࡛పୗࡋࡓࠋཷ⫾㤿࡛ࡣࠊศፔᚋ ᪥ࡲ࡛㧗್ࢆ⥔ᣢࡋ࡚పୗࡋࠊศ ፔᚋ ᪥ࡲ࡛పୗࢆ♧ࡋࡓࡀࠊࡑࡢᚋ෌ࡧୖ᪼ࡋࡓࠋ 㸲㸲㸲㸲㸫㸱㸫㸳㸬ࢸࢫࢺࢫࢸࣟࣥ㸫㸱㸫㸳㸬ࢸࢫࢺࢫࢸࣟࣥ㸫㸱㸫㸳㸬ࢸࢫࢺࢫࢸࣟࣥ㸫㸱㸫㸳㸬ࢸࢫࢺࢫࢸࣟࣥ)LJ)LJ)LJ)LJ ⾑୰ࢸࢫࢺࢫࢸࣟࣥ⃰ᗘࡣࠊ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡢኚ໬࡜ྠᵝ࡞ኚ໬ ࢆ♧ࡋࡓࠋ⾑୰ࢸࢫࢺࢫࢸࣟࣥ⃰ᗘࡣࠊศፔᚋ㏿ࡸ࠿࡟పୗࡋࠊศፔᚋ ᪥௨ 㝆᤼༸๓᪥ࡢศፔᚋ ᪥ࡲ࡛ᇶᗏ್ࢆ⥔ᣢࡋࡓࠋ᤼༸ᚋࡣࠊᛴ⃭࡟ୖ᪼ࡋࠊཷ ⫾㤿࡜୙ཷ⫾㤿࡛ࠊ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡢኚ໬࡜ྠᵝ࡞ኚ໬ࢆ♧ࡋࡓࠋ ࡍ࡞ࢃࡕࠊ୙ཷ⫾㤿࡛ࡣࠊศፔᚋ ᪥ࠊཷ⫾㤿࡛ࡣศፔᚋ ᪥࡟ࣆ࣮ࢡ࡟㐩 ࡋࡓᚋࠊ୙ཷ⫾㤿࡛ࡣࠊศፔᚋ ᪥㸦᤼༸ᚋ ᪥㸧ࡲ࡛㧗್ࢆ⥔ᣢࡋࡓᚋప ୗࡋࠊศፔᚋ ᪥࡟ࡣᇶᗏ್࡟ࡲ࡛పୗࡋࡓࠋཷ⫾㤿࡛ࡣࠊศፔᚋ ᪥ࡲ࡛ 㧗್ࢆ⥔ᣢࡋࠊศፔᚋ ᪥࡟୍ᗘపୗࡍࡿഴྥࢆ♧ࡋࡓࡀࠊࡑࡢᚋ෌ࡧୖ᪼ࡋ ࡚㧗್ࢆ⥔ᣢࡋࡓࠋ 㸲㸲㸲㸲㸫㸱㸫㸴㸬࢖ࣥࣄࣅࣥ㸫㸱㸫㸴㸬࢖ࣥࣄࣅࣥ㸫㸱㸫㸴㸬࢖ࣥࣄࣅࣥ㸫㸱㸫㸴㸬࢖ࣥࣄࣅࣥ)LJ)LJ)LJ)LJ ⾑୰࢖ࣥࣄࣅࣥ⃰ᗘࡣࠊศፔᚋ࡯ࡰ୍ᐃࡢᇶᗏࣞ࣋ࣝࢆ⥔ᣢࡋࠊ᤼༸ᚋࡶ኱ ࡁ࡞ኚ໬ࢆㄆࡵࡽࢀࡎࠊ୍ᐃࡢᇶᗏࣞ࣋ࣝࢆ⥔ᣢࡋࡓࠋ᤼༸ᚋࡣࠊཷ⫾㤿ࠊ୙ ཷ⫾㤿ඹ࡟ࠊ⾑୰࢖ࣥࣄࣅࣥ⃰ᗘࡀ᤼༸๓࡟ẚ࡭࡚ప࠸ഴྥࢆ♧ࡋࡓࡀࠊ᭷ᕪ ࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋࡲࡓࠊ᤼༸๓࡜᤼༸ᚋࡢᮇ㛫ࢆ㏻ࡋ࡚ཷ⫾㤿ࡀ୙ཷ⫾㤿

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32 ࡼࡾࡶ⾑୰࢖ࣥࣄࣅࣥ⃰ᗘࡀ㧗࠸ഴྥࢆ♧ࡋࡓࡀࠊ᭷ពᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋ 㸲㸲㸲㸲㸫㸱㸫㸵㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ㸫㸱㸫㸵㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ㸫㸱㸫㸵㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ㸫㸱㸫㸵㸬࢚ࢫࢺࣛࢪ࣮࢜ࣝ)LJ)LJ)LJ)LJ ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘࡣࠊศፔᙜ᪥ࡣࠊSJPO ࢆ㉸࠼ࡿ㧗⃰ᗘࢆ♧ࡋ ࡚㏻ᖖࡢⓎ᝟࿘ᮇ୰ࡢ༸⬊ᮇ࡜ẚ㍑ࡋ࡚⣙ ಸ㏆࠸㧗್ࢆ♧ࡋࡓࠋࡑࡢᚋపୗ ࡋࡓࡀࠊศፔᚋ ᪥ࡲ࡛ࡣࠊSJPO ๓ᚋࡢ㧗⃰ᗘࢆ⥔ᣢࡋࠊ᤼༸᪥࡟ྥ࠿ࡗ ࡚పୗࡋࡓࠋ᤼༸ᚋࡣࠊSJPO ๓ᚋࡢ⃰ᗘࢆ⥔ᣢࡋࡓࡀࠊⓎ᝟࿘ᮇ୰࡟ẚ࡭ ࡚࡞࠾㧗࠸⃰ᗘ࡛࠶ࡗࡓࠋศፔᚋ ᪥ࡲ࡛ࡢ⾑୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘࡢኚ໬ ࡣࠊཷ⫾㤿࡜୙ཷ⫾㤿࡛ࡣᕪࡀㄆࡵࡽࢀ࡞࠿ࡗࡓࠋ 㸲㸲㸲㸲㸫㸱㸫㸶㸬㸫㸱㸫㸶㸬㸫㸱㸫㸶㸬㸫㸱㸫㸶㸬/+/+/+ࠊࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜/+ࠊࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜ࠊࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜࢚ࢫࢺࣛࢪ࣮࢜ࣝࠊࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜࢚ࢫࢺࣛࢪ࣮࢚࢜ࣝࢫࢺࣛࢪ࣮࢚࢜ࣝࢫࢺࣛࢪ࣮࢜ࣝศἪࣃࢱ࣮ࣥࡢẚ㍑ศἪࣃࢱ࣮ࣥࡢẚ㍑ศἪࣃࢱ࣮ࣥࡢẚ㍑ศἪࣃࢱ࣮ࣥࡢẚ㍑ )LJ )LJ )LJ )LJ ศፔᚋ ᪥㛫ࡢ⾑୰ /+ ⃰ᗘ࡜⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡢኚ໬ࢆẚ㍑ࡋࡓ ᅗࢆ )LJ ࡟♧ࡋࡓࠋศፔᚋࠊ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡣࠊపୗࡋ᤼༸ᚋ ࡟ୖ᪼ࡍࡿኚ໬࡜ࠊศፔᚋ⾑୰ /+ ⃰ᗘࡀḟ➨࡟ୖ᪼ࡋ࡚ࠊ᤼༸๓࡟ࣆ࣮ࢡ࡟㐩 ࡋ࡚ࡑࡢᚋ࡟పୗࡍࡿኚ໬ࡀ㏫┦㛵ࡢࣃࢱ࣮ࣥࢆ♧ࡋࠊప࠸㈇ࡢ┦㛵ࡀㄆࡵࡽ ࢀࡓ㸦U 3㸧ࠋศፔᙜ᪥ࡣࣉࣟࢪ࢙ࢫࢸࣟࣥࡀ㧗್࡛࠶ࡗࡓࡀࠊ/+ ࡣప್࡛࠶ࡗࡓࠋࣉࣟࢪ࢙ࢫࢸࣟࣥࡢపୗ࡜ඹ࡟ /+ ࡢୖ᪼ࡀㄆࡵࡽࢀࡓࠋ ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘ࡜࢚ࢫࢺࣛࢪ࣮࢜ࣝ⃰ᗘࡢኚ໬ཬࡧ⾑୰࢚ࢫࢺࣛ ࢪ࣮࢜ࣝ⃰ᗘࡢኚ໬࡜⾑୰ /+ ⃰ᗘࡢኚ໬ࡢ┦㛵ಀᩘࡣࡑࢀࡒࢀࠊࠊ ࡜࡞ࡾࠊ┦㛵㛵ಀࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋ 㸲㸲㸲㸲㸫㸱㸫㸷㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜ࢸࢫࢺࢫࢸࣟࣥࡢศἪࣃࢱ࣮ࣥࡢẚ㍑㸫㸱㸫㸷㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜ࢸࢫࢺࢫࢸࣟࣥࡢศἪࣃࢱ࣮ࣥࡢẚ㍑㸫㸱㸫㸷㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜ࢸࢫࢺࢫࢸࣟࣥࡢศἪࣃࢱ࣮ࣥࡢẚ㍑㸫㸱㸫㸷㸬ࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜ࢸࢫࢺࢫࢸࣟࣥࡢศἪࣃࢱ࣮ࣥࡢẚ㍑)LJ)LJ)LJ)LJ ศፔ࠿ࡽ ᪥ᚋࡲ࡛ࡢ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘ࡜⾑୰ࢸࢫࢺࢫࢸࣟࣥ⃰ᗘ

(41)

33 ࢆẚ㍑ࡋࡓᅗࢆ )LJ ࡟♧ࡋࡓࠋ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘ࡜⾑୰ࢸࢫࢺࢫ ࢸ ࣟ ࣥ ⃰ ᗘ ࡢ ኚ ໬ ࡣ ࠊ ᴟ ࡵ ࡚ 㢮 ఝ ࡋ ࡚ ࠾ ࡾ ࠊ ṇ ࡢ ┦ 㛵 ࡀ ㄆ ࡵ ࡽ ࢀ ࡓ 㸦U 3㸧ࠋ⾑୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘ࡜⾑୰ࢸࢫࢺࢫࢸࣟࣥ⃰ᗘࡣࠊ ศፔᚋ ᪥௨ෆ࡟ᇶᗏ್࡟పୗࡋࠊ᤼༸ᚋ࡟ୖ᪼ࡍࡿᖹ⾜ࡋࡓศἪࣃࢱ࣮ࣥࢆ ♧ࡋࡓࠋ

サラブレット種雌馬の生殖周期各ステージにおける内分泌調節機構に関する研究

ࢧࣛࣈࣞࢵࢻ✀㞤㤿ࡢ

ࢧࣛࣈࣞࢵࢻ✀㞤㤿ࡢ

ࢧࣛࣈࣞࢵࢻ✀㞤㤿ࡢ

ࢧࣛࣈࣞࢵࢻ✀㞤㤿ࡢ

⏕Ṫ࿘ᮇྛࢫࢸ࣮ࢪ࡟࠾ࡅࡿ

⏕Ṫ࿘ᮇྛࢫࢸ࣮ࢪ࡟࠾ࡅࡿ

⏕Ṫ࿘ᮇྛࢫࢸ࣮ࢪ࡟࠾ࡅࡿ

⏕Ṫ࿘ᮇྛࢫࢸ࣮ࢪ࡟࠾ࡅࡿ

ෆศἪㄪ⠇ᶵᵓ࡟㛵ࡍࡿ◊✲

ෆศἪㄪ⠇ᶵᵓ࡟㛵ࡍࡿ◊✲

ෆศἪㄪ⠇ᶵᵓ࡟㛵ࡍࡿ◊✲

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