Acta Medica Okayama
Volume 55, Issue 2 2001 Article 6
A PRIL 2001
In vitro transformation of rat renal cells by treatment with ferric nitrilotriacetate.
Chikako Kakehashi
∗Masaharu Mori
†Teruyuki Kawabata
‡Shigeru Okada
∗∗∗Okayama Prefectural University,
†Okayama Prefectural University,
‡Okayama University,
∗∗Okayama University,
Copyright c1999 OKAYAMA UNIVERSITY MEDICAL SCHOOL. All rights reserved.
In vitro transformation of rat renal cells by treatment with ferric nitrilotriacetate. ∗
Chikako Kakehashi, Masaharu Mori, Teruyuki Kawabata, and Shigeru Okada
Abstract
Administration of ferric nitrilotriacetate (Fe-NTA) in vivo causes acute renal tubular injury and finally induces renal cell carcinoma. There is accumulating evidence that these processes involve free radicals generated by Fe-NTA. To study the mechanism of renal carcinogenesis by Fe-NTA, we attempted to induce malignant transformation of primary cultured renal cells by treatment with Fe-NTA. When primary cultured renal cells (PRC) were treated continuously with Fe-NTA, all of the PRC died without transformation. On the other hand, when PRC were treated intermittently with Fe-NTA, transformed epithelial colonies were observed at 3 weeks after the first treatment. The established transformed cell line (RK523) showed drastic morphological transfor- mation, grew in soft agar, and formed tumors when transplanted into athymic nude mice. These results indicate that the balance between cytotoxicity and mutagenecity is important for Fe-NTA induced transformation. The RK523 cell line may be a useful model for studying renal carcino- genesis in vitro.
KEYWORDS:renal cell, Fe-NTA(ferric nitrilotriacetate), malignant transformation, in vitro
∗PMID: 11332205 [PubMed - indexed for MEDLINE]
Copyright (C) OKAYAMA UNIVERSITY MEDICAL SCHOOL
1 Kakehashi et al.: In vitro transformation of rat renal cells by treatment with ferr
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2 Acta Medica Okayama, Vol. 55 [2001], Iss. 2, Art. 6
http://escholarship.lib.okayama-u.ac.jp/amo/vol55/iss2/6
3 Kakehashi et al.: In vitro transformation of rat renal cells by treatment with ferr
Produced by The Berkeley Electronic Press, 2001
4 Acta Medica Okayama, Vol. 55 [2001], Iss. 2, Art. 6
http://escholarship.lib.okayama-u.ac.jp/amo/vol55/iss2/6
5 Kakehashi et al.: In vitro transformation of rat renal cells by treatment with ferr
Produced by The Berkeley Electronic Press, 2001
6 Acta Medica Okayama, Vol. 55 [2001], Iss. 2, Art. 6
http://escholarship.lib.okayama-u.ac.jp/amo/vol55/iss2/6
7 Kakehashi et al.: In vitro transformation of rat renal cells by treatment with ferr
Produced by The Berkeley Electronic Press, 2001
