研究業績・活動報告2008

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(1)東北大学多元物質科学研究所. 研究業績・活動報告 2008 年 (平成 20 年)12 月. Institute of the Multidisciplinary Research for Advanced Materials Tohoku University Sendai, Japan.

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(3) 多元物質科学研究所. 研究業績・活動報告目次研究活動報告 ·········································································· 多元設計研究部門 ·············································································································. 1 1. 多元制御研究部門 ············································································································· 31 多元解析研究部門 ············································································································· 43 融合システム研究部門 ······································································································· 64 資源変換・再生研究センター ······························································································ 76 先端計測開発センター ······································································································· 96 多元ナノ材料研究センター ································································································· 104 新産業創造物質基盤技術研究センター ·················································································· 114 窒化物ナノ・エレクトロニクス材料研究センター ··································································· 116 寄附研究部門・研究教授・多元研フェロー・研究ステーション・技術室 ····································· 126. 研究会報告 ············································································· 142 学会発表講演目録 ···································································· 178 多元設計研究部門 ············································································································· 178 多元制御研究部門 ············································································································· 206 多元解析研究部門 ············································································································· 221 融合システム研究部門 ······································································································· 235 資源変換・再生研究センター ······························································································ 251 先端計測開発センター ······································································································· 259 多元ナノ材料研究センター ································································································· 264 新産業創造物質基盤技術研究センター ·················································································· 275 窒化物ナノ・エレクトロニクス材料研究センター ··································································· 276 寄附研究部門・研究教授・多元研フェロー・技術室 ································································ 285. 研究業績目録 ·········································································· 289 多元設計研究部門 ············································································································· 289 多元制御研究部門 ············································································································· 299 多元解析研究部門 ············································································································· 306 融合システム研究部門 ······································································································· 313 資源変換・再生研究センター ······························································································ 318 先端計測開発センター ······································································································· 326 多元ナノ材料研究センター ································································································· 328 新産業創造物質基盤技術研究センター ·················································································· 331 窒化物ナノ・エレクトロニクス材料研究センター ··································································· 333 寄附研究部門・研究教授・多元研フェロー・技術室 ································································ 336. 業績目録著者索引 ···································································· 341.

(4) 1. 研究活動報告. 䇼⎇ⓥᵴേႎ๔䇽㩷 ᢎ. 㩷. ⴫㕙ᯏ⢻⸳⸘⎇ⓥಽ㊁㧔㨪㧕㩷. ᝼㧦㩷 ৻⦡㩷 ታ㩷. ಎ ᢎ ᝼㧦㩷 ਃ᧛⠹ม㩷 ഥ ᢎ㧦㩷 ₺㩷 ศ⼾㪃㩷 ᛂ⿧㓷ੳ㩷 ฃ⸤⎇ⓥຬ㧦㩷㊄ේᱜౖ㩷 ඳ჻⎇ⓥຬ㧦㩷 ⵹ବ૓㪃㩷 ᵩ㩷 ⋧ノ㩷 ᄢ ቇ 㒮 ↢㧦㩷 ᧘శΐ㪃㩷 ⮮↰ஜ⾗㪃㩷 ቟የᥙᒾ㪃㩷 ᐔ⵨ሶ㪃㩷㊄ትਛ㪃㩷 ᔒ↰㓶ᄥ㪃㩷㩷 ᳗ᯅᶈੑ㪃㩷㚍႐␭ᰴ㪃㩷 ട⮮⾫৻㪃㩷 ౎ᧁᵗశ㪃㩷 ጊ↰᣿ᓼ㪃㩷㩷 ࡉࠕࠬ࠶ࠢ̒ࠕࠞ࡜ࡢ࠶࠻㩷 ⎇ ⓥ ↢㧦㩷 ᷰㆻବᴦ㩷㩷 ᧄ⎇ⓥಽ㊁ߢߪ‫ޔ‬㊄ዻ߅ࠃ߮ൻว‛ඨዉ૕ࠍኻ⽎ߦ‫ޔ‬ਥߣߒߡ⿥㜞⚐ᐲ⚛᧚૞⵾ߩߚ߼ߩᣂߒ޿♖ ⵾ᚻᴺ߅ࠃ߮ࡊࡠ࠮ࠬߩ㐿⊒‫ޔ‬㜞⚐ᐲ⚛᧚ߩ․ᕈ⸃᣿‫․ࠢ࡞ࡃ߮ࠃ߅⤑⭯ޔ‬ᕈߦਈ߃ࠆਇ⚐‛ߩᓇ㗀 ⸃᣿╬ߦߟ޿ߡ⎇ⓥᵴേࠍⴕߞߡ޿ࠆ㧚㪉㪇㪇㪏ᐕߩ⎇ⓥᵴേߣߒߡߪ㧘એਅߩࠃ߁ߦ᭎᜝ߐࠇࠆ㧚㩷㩷䋱䋮Ꮧ࿯㘃㊄ዻ䈱㜞⚐ᐲൻ䈮㑐䈜䉎⎇ⓥ㩷 Ꮧ࿯㘃㊄ዻߪ‫․ߩߘޔ‬ᕈߩᄙ᭽ᕈ߆ࠄߐ߹ߑ߹ߥಽ㊁߳ߩᔕ↪߇ߥߐࠇߡ޿ࠆ‫ޕ‬Ꮧ࿯㘃㊄ዻర⚛ߪ ൻቇ⊛ߦᵴᕈߢ‫⋧ޔ‬੕ಽ㔌߇࿎㔍ߢ޽ࠆߚ߼ߦ㜞⚐ᐲൻ߇ㆃࠇߡ޿ࠆ‫ޕ‬Ꮧ࿯㘃㊄ዻߩ․ᕈ⸃᣿߅ࠃ߮ ᣂߚߥᔕ↪▸࿐ߩ㐿ᜏߩߚ߼ߦߪ㜞⚐ᐲᏗ࿯㘃㊄ዻߩ૞⵾߇ਇนᰳߢ޽ࠆ‫ޔߦߢ߹ࠇߎޕ‬Ⴎ㉄㉄ᕈ᳓ ṁᶧਛߢ᦭ᯏṁᇦ฽ᶐ᮸⢽ࠍ࿕ቯ⋧ߦ↪޿ߚࠞ࡜ࡓᴺߦࠃࠆᏗ࿯㘃㊄ዻߩ⋧੕ಽ㔌ࠍ⋡⊛ߣߒߚၮ␆ ታ㛎ࠍⴕ޿‫ޔ‬LaߣઁߩᏗ࿯㘃㊄ዻర⚛ߣߩಽ㔌ߦᚑഞߒߚ‫ᧄޕ‬ᐕߪ‫ޔ‬૞⵾ߒߚ㜞⚐ᐲLaCl3᳓ṁᶧ߆ࠄ‫ޔ‬ ࠪࡘ࠙㉄ᴉᲚᴺ෸߮ᾁ὾ߦࠃࠅ㜞⚐ᐲLa2O3ࠍ૞⵾ߒߚ‫ޕ‬ᓧࠄࠇߚ㜞⚐ᐲLa2O3ߩ⚐ᐲߪ99.996 %ߢ޽ࠅ‫ޔ‬ Ꮧ࿯㘃㊄ዻߩߺࠍ⠨ᘦߒߚ႐วߩ⚐ᐲߣߒߡߎࠇ߹ߢߩ୯ࠍ਄࿁ࠆ99.9998 %ࠍ㆐ᚑߒߚ‫ޕ‬ 䋲䋮䌈䌦䈫䌚䌲䈫䈱⋧੕ಽ㔌䈮㑐䈜䉎⎇ⓥ㩷 HfO2䉇ZrO2䈲La2O3╬䈫౒䈮ᰴ਎ઍULSI䈱䉭䊷䊃⛘✼⤑᧚ᢱ䈫䈚䈩ᦼᓙ䈘䉏䈩䈇䉎䇯ULSI䈮ઍ⴫䈘䉏䉎㔚 ሶ䊂䊋䉟䉴䈮૶↪䈘䉏䉎᧚ᢱ䈲㜞⚐ᐲ䈪䈅䉎੐䈏ⷐ᳞䈘䉏䉎䇯䈚䈎䈚䈭䈏䉌䇮HfO2䉇ZrO2䈱ේ᧚ᢱ䈪䈅䉎Hf䉇 Zr䈱⚐ᐲ䈲⃻⁁䈪లಽ䈮㜞䈇䈫䈲⸒䈋䈭䈇䇯․䈮Hf䈫Zr䈱⋧੕ಽ㔌䈲㔍䈚䈒䇮Ꮢ⽼㜞⚐ᐲຠ䈮䈍䈇䈩䉅䇮Hf 䈮䈲Zr䈏0.1 mass%⒟ᐲ䇮Zr䈮䈲100 mass ppm⒟ᐲ䈱Hf䈱ᷙ౉䈏⷗䉌䉏䉎䇯䈠䈖䈪䇮ᧄ⎇ⓥಽ㊁䈪♽⛔⊛䈮 ⎇ⓥ䈚䈩䈐䈢㒶䉟䉥䊮੤឵♖⵾ᴺ䈮䉋䉎Hf䈫Zr䈱ಽ㔌䉕ᬌ⸛䈜䉎੐䈫䈚䈢䇯੹ᐕᐲ䈲䇮Hf䈫Zr䈱Ⴎ㉄ṁᶧਛ䈮䈍䈔䉎㒶䉟䉥䊮੤឵᮸⢽䈻䈱ๆ⌕᜼േ䉕᣿䉌䈎䈮䈜䉎੐䉕⋡⊛䈫䈚䈢䇯䈠䈱⚿ᨐ䇮ฦ䇱䈱ᐔⴧಽ㈩ଥᢙ䈲ᓥ ᧪ႎ๔䈘䉏䈩䈇䉎୯䉋䉍䉅ዊ䈘䈇੐䈏ಽ䈎䉍䇮䉁䈢䇮ಽ㔌䈮ᦨㆡ䈭Ⴎ㉄Ớᐲ䉕᣿䉌䈎䈮䈜䉎੐䈏಴᧪䈢䇯ᓧ䉌䉏䈢⚿ᨐ䉕ၮ䈮䉦䊤䊛ᴺ䈮䉋䉎ಽ㔌䉕⹜䉂䈢⚿ᨐ䇮Hfਛ䈱Zr䉕1/20䈮ᷫ䈝䉎੐䈱಴᧪䉎䊒䊨䉶䉴䉕㐿⊒䈚䈢䇯ૉ 䈚䇮࿁෼₸䈏80 %䈫ૐ䈇䈢䉄䇮੹ᓟ䈲♖⵾ല₸෸䈶࿁෼₸䈱ะ਄䉕⋡⊛䈫䈚䈩⎇ⓥ㐿⊒䉕ⴕ䈉䇯䋳䋮㜞⚐ᐲ㌃㔚⌕⤑䈮㑐䈜䉎⎇ⓥ㩷 ᰴ਎ઍ7.5+ߩታ⃻ߦߪ‫ޔ‬%W㈩✢᏷ߩᓸ⚦ൻ PO ߦ઻߁㈩✢ᛶ᛫Ⴧᄢߦࠃࠆ㈩✢ㆃᑧ߇ᄢ߈ߥ⺖㗴ߢ޽ࠆ‫ߦࠇߎޕ‬ኻߒ‫ޔ‬%W߼ߞ߈㈩✢ߩ⿥㜞⚐ᐲൻࠍ࿑ࠅ‫ߦࠄߐޔ‬%W㈩✢⚿᥏☸ᓘߩဋ৻࡮☻ᄢൻࠍ ⴕ߁ߎߣߢ‫ޔ‬㈩✢ᛶ᛫ૐᷫ߇ᦼᓙߐࠇࠆ‫੹ޕ‬ᐕᐲߪ‫⿥ޔ‬㜞⚐ᐲ%W㈩✢ߩ߼ߞ߈ᶎߦᔅⷐߥ㜞⚐ᐲ%Wࠕ ࡁ࡯࠼ߣ㔚⸃ᶎߦ↪޿ࠆ㜞⚐ᐲ%W51ߩ૞⵾ߦߟ޿ߡᬌ⸛ߒߚ‫ޕ‬ᰴᐕᐲߪߎࠇࠄࠍ૶↪ߒ‫ޔ‬㜞⚐ᐲ%W㔚 ⌕⤑ߩ૞⵾ߣ․ᕈߩ⹏ଔࠍⴕ߁‫ޕ‬㩷 .

(5) 研究活動報告. 䋴㩷 Ⴎ㉄㉄ᕈ᳓ṁᶧਛ䈮䈍䈔䉎㍲䉟䉥䊮䈱᜼േ䈮㑐䈜䉎⎇ⓥ㩷ㄭᐕߩඨዉ૕㔚ሶ↥ᬺߦ߅޿ߡ‫ޔ‬㜞⚐ᐲ㊄ዻߩᓎഀߪ߹ߔ߹ߔ㜞߹ߞߡ߈ߡ޿ࠆ‫ޕ‬㜞⚐ᐲ㊄ዻࠍ૞ ⵾ߔࠆᚻᲑߣߒߡḨᑼߦࠃࠆ♖⵾ᴺߪ㊀ⷐߢ޽ࠅ‫ߢ߹ࠇߎޔ‬ᄙߊߩ⎇ⓥ߇ߥߐࠇߡ߈ߚ‫ޕ‬ၮ␆ߣߥࠆ ṁᶧਛߩ㊄ዻ㍲ࠗࠝࡦߩ᜼േߦ㑐ߒߡ߽ᢙᄙߊߩႎ๔߇ߥߐࠇߡ޿ࠆ߇‫ޔ‬ႎ๔୯ߦߪᄢ߈ߥࡃ࡜࠷ࠠ ߇ሽ࿷ߔࠆߣ޿߁໧㗴߇޽ࠆ‫⎇ᧄޕ‬ⓥಽ㊁ߢߪ‫⸃ߦߢ߹ࠇߎޔ‬ᨆᚻᴺߣߒߡ⸘㊂ൻቇ‫▚⸘ޔ‬ൻቇࠍㆡ ↪ߔࠆ੐ߢ‫ࠅࠃޔ‬ታ௝ߦㄭ޿᜼േࠍ⸃᣿ߔࠆᚻᴺࠍ㐿⊒ߒߡ߈ߚ‫ᧄޕ‬ᐕߪ‫ޔ‬㐿⊒ߒߚᚻᴺࠍ↪޿ߡ‫ޔ‬ 㒶ࠗࠝࡦ੤឵᮸⢽߳ߩๆ⌕᜼േ߇ૃㅢߞߡ޿ࠆCuߣCoߦ⌕⋡ߒ‫ޔ‬Ⴎ㉄ṁᶧਛߦ߅ߌࠆฦ‫ࡠࡠࠢߩޘ‬㍲ ૕ߩሽ࿷Ყ₸ࠍ᳞߼ߚ‫ޔߦᦝޕ‬㒶ࠗࠝࡦ੤឵᮸⢽߳ߩๆ⌕ㆊ⒟ߦ߅ߌࠆࠢࡠࡠ㍲૕ߩሽ࿷₸ߩᄌൻ߆ ࠄ‫ޔ‬ๆ⌕ߩ⚛ㆊ⒟ࠍ᣿ࠄ߆ߦߒߚ‫ޕ‬ 㩷䋵㪅㩷㜞⚐ᐲ㪱㫅㪦૞⵾䊒䊨䉶䉴䈱㐿⊒䈫ൻቇ᳇⋧ャㅍᴺ䈮䉋䉎䌚䌮䌏䊋䊦䉪න⚿᥏䈱ᚑ㐳㩷㩷 ⋥ធㆫ⒖ဳඨዉ૕<P1 'I㧩G8㧦ቶ᷷ ߪ⚡ᄖ⊒శ⚛ሶ߿ㅘ᣿⏛ᕈ૕ߣߒߡᦼᓙߐࠇ‫ߚ߹ޔ‬ㅘ᣿ዉ 㔚⤑ߣߒߡ߽᦭ᦸߢ޽ࠆ‫ޔߒ߆ߒޕ‬㨜ဳવዉᕈ೙ᓮ߿ᰳ㒱೙ᓮߩ࿎㔍ᕈ╬ߩ໧㗴ࠍ᦭ߒ‫⸃ߩߘޔ‬᳿ߦ ߪ㜞⚐ᐲ<P1૶↪߇ਇนᰳߢ޽ࠆ‫ߦࠇߎޕ‬ኻߒ೨ᐕᐲߪ‫<ޔ‬Pߩ㒶ࠗࠝࡦ੤឵♖⵾ᴺࠍㆡ↪ߒ‫ޔ‬㊄ዻਇ⚐ ‛ࠍᭂૐᷫൻߒߚ㜞⚐ᐲ<P%NႮ㉄᳓ṁᶧࠍ࿁෼ᓟ‫ޔ‬ਛ๺ᴉᲚ‫ࠈޔ‬ㆊ‫߆ޔ‬὾╬߆ࠄߥࠆ㜞⚐ᐲ<P1૞⵾ࡊ ࡠ࠮ࠬࠍ᭴▽ߒߚ‫ᧄޕ‬ᐕᐲߪ‫ޔ‬ᄢဳ㒶ࠗࠝࡦ੤឵ࠞ࡜ࡓࠍ↪޿ߡ㜞⚐ᐲ<P1૞⵾ࡊࡠ࠮ࠬࠍታᣉߒ‫ޔ‬㜞 ⚐ᐲ<P1☳ᧃࠍ૞⵾ߒ‫ޔ‬ൻቇ᳇⋧ャㅍᴺߦࠃࠆ<P1න⚿᥏ᚑ㐳ߩේᢱߦ↪޿ߚ‫ޕ‬㩷㩷䋶㪅㩷㕖⾰㊂ಽ㔌ဳ䉟䉥䊮䊎䊷䊛䊂䊘䉳䉲䊢䊮ᴺ䈫䈠䈱ᓟ䈱㉄ൻಣℂ䈮䉋䉎㉄ൻ䌈䌦⭯⤑䈱૞⵾䈍䉋䈶⹏ଔ䈮㑐䈜䉎⎇ⓥ㩷㩷㩷 ᧄ⎇ⓥಽ㊁ߢߪ‫ߢ߹ࠇߎޔ‬㜞ຠ૏ࠫ࡞ࠦ࠾࠙ࡓ㉄ൻ⤑ߩ૞⵾ࠍ⋡⊛ߣߒߡ‫ޔ‬᳓⚛ࡊ࡜࠭ࡑṁ⸃ߦࠃ ߞߡ㜞⚐ᐲൻߒߚࠫ࡞ࠦ࠾࠙ࡓ࠲࡯ࠥ࠶࠻ࠍ↪޿‫ࡦ࡚ࠪࠫࡐ࠺ࡓ࡯ࡆࡦࠝࠗޔ‬ᴺߦࠃࠅ⭯⤑ࠍᒻᚑߒ‫ޔ‬ ၮ᧼ࡃࠗࠕࠬ߅ࠃ߮࠲࡯ࠥ࠶࠻⚐ᐲ߇ၸⓍ⤑ߩ⚵❱߅ࠃ߮⚐ᐲߦਈ߃ࠆᓇ㗀ࠍ᣿ࠄ߆ߦߒߡ߈ߚ‫ᧄޕ‬ ᐕߪ‫ޔ‬ᤓᐕ߹ߢߩ⚿ᨐࠍၮߦ‫ޔ‬หᚻᴺࠍ㪟㪽ߦㆡ↪ߒ‫ޔ‬ห᭽ߩലᨐࠍᓧࠆߎߣ߇ߢ߈ߚ‫ޔߦࠄߐޕ‬㪟㪽㪆㪧㫋㪆㪪㫀㪦㧞ࠍૐ᷷㉄ൻᓟ‫ޔ‬㪧㫋ࠍၸⓍߐߖࠆߎߣߢ‫ޔ‬㪧㫋㪆㩷㪟㪽㪦㧞㪆㪧㫋㪆㪪㫀㪦㧞᭴ㅧࠍ૞⵾ߒ‫ޔ‬㔚᳇․ᕈࠍ⹏ଔߒߚ‫ޕ‬ ㉄⚛㔓࿐᳇ਛߢടᾲᤨߦ⚡ᄖ✢ࠍᾖ኿ߔࠆߎߣߢ‫ޔ‬ᓧࠄࠇࠆ㉄ൻ⤑ߩ⺃㔚₸ߩะ਄ߒ‫ࠢ࡯࡝ޔ‬㔚ᵹ߇ ૐਅߔࠆߎߣࠍ᣿ࠄ߆ߦߒߚ‫ޕ‬㩷䋷䋮㌃䈱ૐ᷷㉄ൻ䈮㑐䈜䉎⎇ⓥ㩷 ᭂ߼ߡ⭯޿㉄ൻ⤑ෘߩ᷹ቯߦ᦭↪ߥಽశࠛ࡝ࡊ࠰ࡔ࠻࡝࡯ᴺࠍ↪޿‫ޔ‬㌃න⚿᥏㧔㧕㧔㧕㧔㧕㕙߅ࠃ߮⚐ᐲߩ⇣ߥࠆ㌃ᄙ⚿᥏ߩૐ᷷㉄ൻ᜼േ 㨪͠‫ޔ‬᳇࿶㉄⚛‫ޔ‬#T 1ࠟࠬਛKPUKVW᷹ቯ ࠍ ᬌ⸛ߒߚ‫ޕ‬න⚿᥏ߩ႐ว‫㉄ޔ‬ൻㅦᐲߪ Ҍ 㧪 ߣߥࠅ‫ߚ߹ޔ‬න⚿᥏‫ޔ‬ᄙ⚿᥏ߣ߽ߦ㉄ൻᯏ ᭴ߦ⤑ෘଐሽ߇⹺߼ࠄࠇ‫⚂ޔ‬POએਅߢߪㅒኻᢙೣ‫ޔ‬㨪POߢߪ߶߷ਃਸ਼ೣ‫⚂ޔ‬POએ਄ߢߪ᡼‛ ✢ೣߦᓥ߁ߎߣࠍ᣿ࠄ߆ߦߒߚ‫ޔߚ߹ޕ‬ૐ㉄⚛࿶ਛߢߪో૕⊛ߦ㉄ൻㅦᐲߪૐਅߔࠆ߇‫㉄ޔ‬ൻㅦᐲೣ ߪ೨⸥ߣห᭽ߥ⤑ෘଐሽߦᓥ߁ߎߣࠍ⏕⹺ߒߚ‫ޕ‬㩷㩷䋹㪅㩷 ᡼኿✢ᬌ಴ེ↪ൻว‛ඨዉ૕䈱㜞⚐ᐲൻ䈮㑐䈜䉎⎇ⓥ㩷 ⃻࿷‫ޔ‬ක≮ಽ㊁ߦ߅޿ߡ:✢᠟ᓇᤨߩૐⵍ῜✢㊂ൻ߅ࠃ߮࠺ࠫ࠲࡞ൻ߇ㅴ߼ࠄࠇߡ߅ࠅ‫࠻࠶࡜ࡈޔ‬ ࡄࡀ࡞᡼኿✢ᬌ಴ེߩ㐿⊒߇ㅴ߼ࠄࠇߡ޿ࠆ‫ޕ‬᡼኿✢ᬌ಴ེ↪᧚ᢱߦߪ‫ޔ‬㜞ᛶ᛫߆ߟࠠࡖ࡝ࠕߩ㜞⒖ േᐲ߇ⷐ᳞ߐࠇࠆߚ߼‫ޔ‬㜞⚐ᐲൻ߇ᔅⷐߣߐࠇࠆ‫ޕ‬2D+ߪᬌ಴․ᕈߩ㜞ᗵᐲൻߩⷐ࿃ߢ޽ࠆේሶ⇟ภ߇ ᄢ߈ߊ‫ޔ‬᡼኿✢ᬌ಴ེ↪᧚ᢱߣߒߡᵈ⋡ߐࠇߡ޿ࠆ߇‫ޔ‬Ꮢ⽼᧚ᢱߩ⚐ᐲߪૐ޿ߩ߇⃻⁁ߢ޽ࠆ‫ᧄޕ‬ᐕ ᐲߪ‫*ޔ‬9'ᴺߦࠃࠅ⭯⤑ᒻᚑࠍ⹜ߺࠆߣߣ߽ߦ‫ޔ‬ේᢱ⚐ᐲ߇᡼኿✢ᬌ಴․ᕈߦਈ߃ࠆᓇ㗀ࠍ᣿ࠄ߆ߦߒ ߚ‫ޕ‬. 2.

(6) 3. 研究活動報告. 䇼⎇ⓥᵴേႎ๔䇽㩷శᯏ⢻⸳⸘⎇ⓥಽ㊁䋨2008䋮1䌾㪉㪇㪇㪏䋮12䋩㩷 ᢎ ᝼㩷㪑๺↰ஜᒾ㩷 ഥ ᢎ㩷㪑⨹ᧁ଻ᐘ㪃㩷 ဈᧄᷡᔒ㩷 ᄢ ቇ 㒮 ↢㩷㪑⍹Ⴆ↱૫ሶ䋬ਛ᧛࿻੺䋬㆙⮮⛗᪸ሶ䋬ዊ㊁ኹ૫ሶ䋬㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷 ኹౝ⟤㚅䋬᧛਄㩷 ᘕ㩷 ⎇ ⓥ ຬ㩷㪑૒⮮ᓀട㩷㩷. 㩷. 㩷. 㩷. ᧄ⎇ⓥಽ㊁䈪䈲 DNA 䉇 RNA䇮䈠䈚䈩䉺䊮䊌䉪⾰䈭䈬↢૕㜞ಽሶ䈱ᰴ਎ઍ䉟䊮䊁䊥䉳䉢䊮䊃ဳ䊅䊉䊋䉟䉥ᯏ ⢻᧚ᢱ䈻䈱ᔕ↪䉇శᯏ⢻᧚ᢱ䈻䈱ዷ㐿䉕⋡ᜰ䈚䇮⎇ⓥᵴേ䉕ⴕ䈦䈩䈇䉎䇯2008 ᐕ䈱⎇ⓥᵴേ䈫䈚䈩䈲䇮એਅ 䈱䉋䈉䈮᭎᜝䈘䉏䉎䇯㩷㪈㪅㩷 ᩭ㉄⹺⼂․ᕈ䊶⚦⢩⤑ㅘㆊᕈะ਄䉕⋡ᜰ䈚䈢䊕䊒䉼䊄䊥䊗ᩭ㉄䈻䈱䉝䊦䉩䊆䊮ዉ౉ലᨐ䈮㑐䈜䉎⎇ⓥ 㩷 ᚒ䇱䈲䇮䈏䉖⚦⢩․⇣⊛ㆮવሶᴦ≮⮎䈱ഃᚑ䉕⋡ᜰ䈚䇮䊖䉡㉄⺃ዉ૕ሽ࿷ਅ䇮pH 䉕ᄖㇱ࿃ሶ䈫䈚䈢ᩭ㉄ Ⴎၮㇱ䈱 anti 㹖 syn ㈩ะ೙ᓮ䉕㚟േജ䈫䈚䇮mRNA 䈮ኻ䈜䉎นㅒ⊛㍲૕ᒻᚑ⢻䉕᦭䈜䉎䊕䊒䉼䊄䊥䊗ᩭ㉄ (PRNA)䉕ႎ๔䈚䈩䈐䈢 1䇯PRNA 䈲䈏䉖․᦭䈱⚦⢩⾰᧦ઙਅ䈪䈱䉂⮎ല䉕⊒⃻䈚䇮ᰴ਎ઍ䈱䈏䉖⚦⢩․⇣⊛ ㆮવሶᴦ≮⮎䈫䈚䈩㜞䈇น⢻ᕈ䉕᦭䈜䉎䈏䇮ലᨐ⊛䈭⮎೷䈮ᔅⷐਇนᰳ䈭㜞䈇⚦⢩⤑ㅘㆊᕈ䈮㑐䈜䉎ᯏ⢻ ઃട䈏䇮ᧂ䈣චಽ䈫䈲⸒䈋䈭䈇⁁ᴫ䈪䈅䈦䈢䇯৻ᣇ䇮㜞䈇⚦⢩⤑ㅘㆊᕈ䉕᦭䈜䉎 Tat 䊕䊒䉼䊄䇮䈠䈱ਥⷐ᭴ᚑ 䉝䊚䊉㉄䈪䈅䉎䉝䊦䉩䊆䊮(Arg)䉥䊥䉯䊙䊷䉕⤑ㅘㆊᕈ䊔䉪䉺䊷䈫䈚䈩↪䈇䇮⮎‛䉇䉺䊮䊌䉪⾰䈭䈬䈱㜞ല₸⚦ ⢩ౝዉ౉ᴺ䈏ᦨㄭႎ๔䈘䉏ᵈ⋡䈘䉏䈩䈇䉎 2䇯䈖䈱䉋䈉䈭⢛᥊䉕〯䉁䈋䈩䇮ᧄᐕᐲ䈲⚦⢩ዉ౉ല₸䈱㜞䈇ᣂⷙ PRNA 㐿⊒䉕⋡ᜰ䈚䇮䉝䊦䉩䊆䊮䉕ಽሶౝ䈮ዉ ౉䈚䈢DPRNA 䉕⸳⸘䊶วᚑ䈚䇮DNA 䈭䉌䈶䈮 RNA 䈫䈱㍲૕ᒻᚑ⢻䇮䈍䉋䈶ᄖㇱ࿃ሶ䈮䉋䉎㍲૕䈱ᒻᚑ䊶⸃㔌೙ ᓮ䉕ᬌ⸛䈚䈢䇯䈘䉌䈮㱍PRNA 䈱⚦⢩ዉ౉ല₸䈮䈧䈇䈩䉅⹦⚦䈮ᬌ⸛䈚䈢䇯. Figure 1. Structure ofҏDPRNA and interaction of DPRNA with mRNA. ⚿ᨐߣ⠨ኤ㧦วᚑ㧦ᣢႎ䈮ᓥ䈇䇮DPRNA 䊝䊉䊙䊷䉕วᚑ䈚䈢䇯DPRNA 䊝䊉䊙䊷䈫 Fmoc-Arg(Pbf)-OH䇮䉕↪ 䈇䇮 Fmoc ࿕ ⋧ ว ᚑ ᴺ 䈮䉋䉍 DPRNA 䈫䉝䊦䉩䊆䊮䈱 ੤ ੕ ㈩ ೉ 䉕 ᦭ 䈜䉎 PRNA8 ㊂ ૕ 䉥䊥䉯䊙䊷 (㱍PRNA1)䉕วᚑ䈚䇮HPLC 䈮䉋䉍න㔌䊶♖⵾䉕ⴕ䈦䈢ᓟ䇮MALDI-TOF MS 䈮䉋䉍หቯ䈚䈢䇯㍲૕ᒻᚑ⢻㧦㱍PRNA1 䈫 DNA 䈫䈱㍲૕ᒻᚑ᜼േ䉕䇮UV 䉴䊕䉪䊃䊦䈮䉋䈦䈩ᬌ⸛䈚䈢䇯ૐႮỚᐲ᧦ઙਅ䈮䈍䈇䈩䇮㍲૕䈱቟ቯᕈ䈱ᜰᮡ䈫䈭䉎Ⲣ⸃᷷ᐲ(Tm) 䈲䇮DNA ㍲૕䈮Ყ䈼 50 ᐲએ਄㜞䈒䇮㕖Ᏹ䈮቟ቯ䈭㍲૕䉕ᒻ ᚑ䈪䈐䉎䈖䈫䈏᣿䉌䈎䈫䈭䈦䈢(⴫ 1)䇯䉁䈢䇮ǩPRNA1 䈲 1 Ⴎၮ䊚䉴䊙䉾䉼ዉ౉ DNA 䈫䈲㍲૕䉕ᒻᚑ䈞䈝䇮㜞䈇Ⴎၮ㈩೉⹺⼂ᕈ䉕᦭䈜䉎䈖䈫䈏␜䈘䉏䈢䇯䈘䉌䈮 20䌭M 䈱䊖䉡㉄ᷝട䈮䉋䉍ㄦㅦ䈎䈧ല₸䉋䈒㍲૕⸃㔌䈏⺃ ⿠䈘䉏䇮䉧䊮⚦⢩․⇣⊛ㆮવሶᴦ≮⮎䈫䈚䈩ఝ䉏䈢․ᕈ䉕᦭䈜䉎䈖䈫䈏᣿䉌䈎䈫䈭䈦䈢䇯 ǩPRNA 12 ㊂૕㧦ታ↪⊛䈭ㆮવሶᴦ≮⮎䈮᳞䉄䉌䉏䉎ᮡ⊛ RNA 䈮ኻ䈜䉎ㆬᛯᕈ䉕ะ਄䈘䈞䉎䈢䉄䇮PRNA.

(7) 研究活動報告. 12 ㊂૕วᚑ䈮 Table 1. Melting temperatures of DPRNA oligomers and DNAs with DNA/RNA ข䉍⚵䉖䈣䇯ǩ 㩷㩷 Tm/㰛C 㩷㩷 PRNA1 ห᭽䈱 Oligomer Sequence Target DNA or RNA 㩷㩷 +borax ᭴ㅧ䉕᦭䈜䉎 12 a ㊂ ૕ ( ǩ R-URURURURCRURURCR 5’-d(GAAGAAAA) 53 DPRNA1 PRNA2) 䉕 ว 5’-d(AAAAGAAG) 57 <5 㩷㩷 ᚑ 䈚䈢䈏䇮⋧⵬ 5’-d(GAATAAAA) <5 㩷㩷㎮䈱 DNA 䈫᳓ 5’-d(TAAGAAAA) <5 㩷㩷 ṁᕈ䈱ਲ䈚䈇䊘 b 䊥䉟䉥䊮䉮䊮䊒䊧 DNA1 5'-d(TTTTCTTC) 5’-d(GAAGAAAA) <5 <5 䉾䉪䉴䉕ᒻᚑ䈜 a R-(UR)12 5'-d(A)12 㺍 -㩷 DPRNA2 䉎䈖䈫䈏᣿䉌䈎䈫 c DPRNA3 R-USURCSURUSURUSCRUSURUSUR 5'-d(AAAAGAAAAGAA) 37 䈭䈦䈢䇯䈖䉏䈲 13 ᱷၮዉ౉䈚䈢 5'-r(AAAAGAAAAGAA) 48 <20 㩷㩷 Arg 䈱㜞䈇ᱜ㔚 c <10 DPRNA4 R-USUSCSURURURURCRUSUSUSUR 5'-d(AAAAGAAAAGAA) 17 ⩄䈫䇮䊥䊮㉄⽶㔚 b DNA2 5'-d(TTCTTTTCTTTT) 5'-d(AAAAGAAAAGAA) 21 ⩄㑆䈪䈱㕒㔚⋧ ੕૞↪䈮ၮ䈨䈒 Measured in a ) 33 PM phosphate buffer at pH 7.2, b) 33 mM phosphate buffer at pH 7.2, c) 33 䈫 ⠨ 䈋䇮㕒㔚 ⋧ mM Tris-HCl at pH 7.2. UR : DPRNA(U)-Arg, US : DPRNA(U)-Ser, CR : DPRNA(C)-Arg , CS : DPRNA(C)-Ser. ੕૞↪✭๺䉕⋡ ⊛䈮䇮ਛᕈ䈪᳓ṁᕈ䈏㜞䈇䉶䊥䊮ᱷၮ䈱㱍PRNA 䈻䈱ዉ౉䉕ᬌ⸛䈚䈢䇯Ser ᱷၮ䈱ዉ౉ᴺ䈫䈚䈩 Arg 䈫䈱੤੕ ዉ౉ဳ (DPRNA3)䇮䈍䉋䈶䊑䊨䉾䉪ဳ (DPRNA4) 䈱 2 ⒳䉕วᚑ䈚䇮DNA 䈍䉋䈶 RNA 䈫䈱㍲૕ᒻᚑ᜼േ䉕 ᬌ⸛䈚䈢䇯੤੕ዉ౉ဳ䈲ᱜ㔚⩄䈱㓸ਛ䉕ㆱ䈔 RNA 䈫䈱㕒㔚⋧੕૞↪䈱ലᨐ⊛✭๺䈏ᦼᓙ䈘䉏䇮䊑䊨䉾䉪ဳ 䈲 DNA 䈫䈱㍲૕ᒻᚑᤨ䈱ᓞㅦᲑ㓏䈪䈅䉎ᩭᒻᚑᲑ㓏䈮᦭೑䈫䈭䉎䈖䈫䈏ᦼᓙ䈘䉏䉎䇯㩷 33 mM 䈱✭ⴣᶧਛ䈮䈍䈇䈩੤੕ဳDPRNA3 䈫⋧⵬⊛ DNA 䈫䈱㍲૕䈱 Tm 䈲 37㷄䈫䈭䉍䇮DNA ㍲૕䈮Ყセ䈚䈩 16㷄䉅቟ቯ䈭㍲૕䉕ᒻᚑ䈜䉎䈖䈫䈏᣿䉌䈎䈫䈭䈦䈢䇯䈘䉌䈮 RNA 䈫䈱㍲૕䈱 Tm 䈲䇮48㷄䈫䈭䉍䇮ㆮવሶᴦ ≮⮎䈫䈚䈩㊀ⷐ䈭 RNA ㆬᛯ⊛㍲૕ᒻᚑ․ᕈ䉕᦭䈜䉎䈖䈫䉅␜䈘䉏䈢䇯৻ᣇ䇮㩷㩷㱍PRNA4-DNA ㍲૕䈲 DNA ㍲૕䉋䉍䉅 4㷄ૐ䈇 Tm 䈫䈭䉍䇮Arg 䈫 Ser 䈱੤੕ዉ౉ဳ㱍PRNA 䈏ఝ䉏䈢ಽሶ᭴ㅧ䈪䈅䉎䈖䈫䈏᣿䉌䈎䈫䈭䉍䇮 Arg 䈍䉋䈶 Ser ዉ౉㱍PRNA 䈱⸳⸘ᜰ㊎䉕⏕┙䈪䈐䈢䇯 ⚦⢩ዉ౉㧦DPRNA 䉕Ⱟశ⦡⚛䈪ᮡ⼂䈚䇮HeLa ⚦⢩䉕↪䈇⚦⢩⤑ ㅘㆊᕈ䈮䈧䈇䈩ᬌ⸛䈚䈢䇯౒ὶὐⰯశ㗼ᓸ㏜᷹ⷰ䈭䉌䈶䈮䊐䊨䊷䉰䉟䊃䊜䊃䊥䊷᷹ቯ䈮䉋䉍䇮⤑ㅘㆊᕈ䈏㕖Ᏹ䈮㜞䈇 Arg 8 ㊂૕䈫Ყセ䈚䈩䇮䈇䈝䉏䈱DPRNA 䉅 1.5~3.0 ୚䈱⤑ㅘㆊ⢻䉕␜䈚䇮ఝ䉏䈢⚦⢩⤑ㅘ ㆊᕈ䉕᦭䈜䉎䈖䈫䈏᣿䉌䈎䈫䈭䈦䈢(࿑ 2)䇯䈖䈱ఝ䉏䈢⤑ㅘㆊᕈ䈲䇮 Arg 䉥䊥䉯䊙䊷䈮Ყセ䈚䈩䇮㱍PRNA 䈏ㆡᐲ䈭ⷫ䊶⇹᳓䊋䊤䊮䉴䉕᦭ 䈜䉎䈖䈫䈮ၮ䈨䈒䈫⠨䈋䉌䉏䉎䇯䉁䈢䇮MTT 䉝䉾䉶䉟䈱⚿ᨐ䇮⚦⢩ᷝട ᓟ 1 ᤨ㑆ᓟ䈪䈲⚦⢩Ქᕈ䈲ో䈒᷹ⷰ䈘䉏䈝䇮ᭂ䉄䈩ఝ䉏䈢⚦⢩ዉ ౉․ᕈ䉕᦭䈜䉎䈖䈫䈏␜䈘䉏䈢䇯 એ਄䇮ᧄᐕᐲ䈲 RNA ㆬᛯ⊛቟ቯ㍲૕ᒻᚑ⢻䇮䉧䊮⚦⢩․⇣⊛⮎ല⊒ ⃻․ᕈ䇮䈠䈚䈩㜞䈇⚦⢩ዉ౉․ᕈ䉕౗䈰஻䈋䈢ఝ䉏䈢䈏䉖⚦⢩․⇣⊛ Figure 2. Fluorescence images of ㆮવሶᴦ≮⮎䈫䈚䈩น⢻ᕈ䉕᦭䈜䉎੤੕ဳDPRNA 䈱㐿⊒䈮ᚑഞ䈚䇮ᣂ HeLa cell following incubation in D-MEM(+) at 37㰛C for 1h. ⷙ PRNA ⸳⸘ᜰ㊎䉕᭴▽䈚䈢䇯 1) Wada; T., Sato, H., Inoue, Y. Biopolymers 2004, 76, 15. 2) Futaki, S. Adv. Drug Deliv. Rev. 2008, 60, 447.. 4.

(8) 5. 研究活動報告. 㪉㪅㩷 ౞ੑ⦡᷹ቯⵝ⟎䈱ᤨ㑆ಽ⸃⢻ะ਄䈫㪛㪥㪘㪄⮎೷㑆䈱⋧੕૞↪䈱ᤨ㑆ಽ⸃᷹ቯ䉕⋡ᜰ䈚䈢⎇ⓥ㩷㩷 DNA 䈮⚿ว䈜䉎⮎೷䈱⚿ว᭽ᑼ䈮䈲䇮৻⥸䈮䉟䊮䉺䊷䉦䊧䊷䉲䊢䊮䇮䉫䊦䊷䊑䊋䉟䊮䊂䉞䊮䉫╬䈏⍮䉌䉏䈩䈍䉍䇮䈖䉏䉌䈲ᾲ䇮ႮỚᐲ䈭䈬䈱ᄖ⊛࿃ሶ䈫䇮DNA-⮎೷㑆䈱㕒㔚⋧੕૞↪䇮⇹᳓ᕈ⋧੕૞↪䇮㱜-㱜⋧੕૞ ↪䈮䉋䉍ⶄ㔀䈭᭽⋧䉕␜䈜䇯䈘䉌䈮䇮䈖䉏䉌䈱⋧੕૞↪䈲䇮ᾲ䉇ႮỚᐲ䈭䈬䈱ᄖ⊛ⷐ࿃䈪Ᏹ䈮ំ䉌䈇䈪䈇䉎䈫 ⠨䈋䉌䉏䇮䈠䈱േ⊛஥㕙䈮ὶὐ䉕䈅䈩䈢⎇ⓥ䈏䇮DNA䋭⮎೷㑆⋧੕૞↪䈱ᦝ䈭䉎ℂ⸃䈮❬䈏䉍䇮䈵䈇䈩䈲⮎ ೷䈱ᣂⷙ㐿⊒䈻䈫❬䈏䉎䉅䈱䈫ᦼᓙ䈘䉏䉎䇯. Elliptically polarization change due to the sample chirality. Observation of CD. DNA-⮎೷㑆⋧੕૞↪䈱᷹ቯ䈮䈲䇮౞ੑ⦡ (CD)䉴䊕䉪䊃䊦᷹ቯ䈏᦭↪䈭ᖱႎ䉕ਈ䈋䉎䈖䈫䈏⍮䉌䉏䈩䈇䉎䈏䇮Ꮢ⽼䈱 CD ᷹ቯⵝ⟎䈪䈲⋧ ੕૞↪䈱േ⊛᜼േ䈱⸃᣿䈜䈭䉒䈤 CD 䉴䊕䉪䊃. Xe lamp. Q. AP. S. AP. 䊦䈱ᤨ㑆ᄌൻ᷹ቯ䈮䈲ⵝ⟎䈱ᤨ㑆ಽ⸃⢻䈏. I/I. ਇ⿷䈚䈩䈇䉎䇯ᓥ䈦䈩 CD ᷹ቯᴺ䈱ᤨ㑆ಽ⸃⢻. GP. L. GP’. L. 䈱ะ਄䈏 DNA-⮎೷㑆⋧੕૞↪䈱⸃ᨆ䈮㕖Ᏹ 䈮᦭↪䈪䈅䉎䇯䈖䈱ⷰὐ䈎䉌ᚒ䇱䈲䇮䊅䊉⑽䈱 ᤨ㑆ಽ⸃⢻䉕᦭䈜䉎䊧䊷䉱䊷䊐䊤䉾䉲䊠䊖䊃䊥䉲䉴ᴺ䉕ၮ䈮䈚䈢 CD ᷹ቯⵝ⟎䉕᭴▽䈚䇮䈖䉏䉕. Random polarization. Linear polarization. Elliptically polarization. DNA-⮎೷㑆⋧੕૞↪䈱⸃ᨆ䈮↪䈇䉎䈖䈫䉕⠨ ᧄᐕᐲ䈲䇮ᣢႎ 1 䈱ᤨ㑆ಽ⸃౞ CD ᷹ቯⵝ ⟎䉕ၮ䈮䈚䈢᷹ቯⵝ⟎䈱䊒䊨䊃䉺䉟䊒䈏䈾䈿ቢ ᚑ䈚䇮䈖䉏䉕↪䈇䈢 herring sperm DNA 䈫᳓ṁ. Schematic illustration of optics layout and polarization of light for time-resolved CD measurement; L: Lenz, AP: aperture, GP: Glan-laser prism, Q: retarder, S: sample, I/I: image intensfier. ᕈ䊘䊦䊐䉞䊥䊮 (TMPyP) 㑆⋧੕૞↪䈱㜞ᤨ㑆ಽ⸃. CD in a ground state. (a). ᷹ቯ䈮ะ䈔䈩䈱᷹ቯ䉕ⴕ䈦䈢䇯Ꮢ⽼ CD ᕈ᷹ቯⵝ⟎ (JASCO J-820) 䈫⥄૞ⵝ⟎䈱⚿ᨐ䈱Ყセ䉕ⴕ䈇䇮CD 䉴䊕䉪䊃䊦᷹ቯ䈏䈾䈿໧㗴䈭䈒ⴕ䉒䉏䈩䈇䉎䈖䈫䉕⏕⹺. (Ir-Il)/(Ir+Il) 㬍 103. 䈋䈢䇯. 0 Laser-induced CD change. -4 -8. -12. 䉐䇮࿑ 3(a)䈮␜䈜䉋䈉䈮䇮䊧䊷䉱䊷ബ⿠೨䈮᷹ⷰ䈘䉏䈩䈇䈢ၮᐩ⁁ᘒ䈱 TMPyP 䈱⺃⿠ CD 䈱ᷫዋ䈏⏕⹺䈪䈐䈢䇯࿑ 3(b)䈮␜䈜䉋䈉䈮䇮䈖䈱᷹ⷰᤨ㑆ౝ䈪䈲䇮 TMPyP 䈱ၮᐩ⁁ᘒಽሶ䈱ᷫዋ䈏ㆊᷰๆ෼ᴺ䈮䉋䉍ⷰ ᷹䈘䉏䇮䈖䉏䉌䈱⚿ᨐ䈎䉌䇮TMPyP 䈱⺃⿠ CD ᒝᐲᄌ ൻ䈲䇮DNA 䈮⚿ว䈚䈢 TMPyP 䈱ၮᐩ⁁ᘒ䈏ᷫዋ䈚䈢. (b). 'Absorbance㬍103. 䈚䈢䇯䉁䈢 TMPyP 䉕䊅䊉⑽䊧䊷䉱䊷䈪శബ⿠䈚䈢䈫䈖 0 -20 -40 -1. 0. 1. 2. 3. 4. Time / ms. ⚿ᨐ䈫䈚䈩᷹ⷰ䈘䉏䈢䉅䈱䈫⠨䈋䉌䉏䉎䇯੹ᓟ䈲วᚑ Figure 3. (a) laser-induced CD change profile DNA 䉕↪䈇䈢䉋䉍ቯ㊂⊛䈭⼏⺰䈱ዷ㐿䉕ⴕ䈉䇯. observed at 440 nm (b) transient absorption profile. 1.㩷 J. W. Lewis, R. F. Tilton, C. M. Einterz, S. J. observed at 440 nm. sLaser-induced CD change Milder, I. D. Kuntz, D. S. Kliger J. Phys. Chem. 1985, 89, observed by our time-resolved CD spectrometer. 289-294..

(9) 研究活動報告. 㩷㪊㪅㩷. 㩷 ᄖㇱೝỗᔕ╵ဳ䉺䊮䊌䉪⾰䈱᭴▽䈫Ⱟశᬌ಴䈻䈱ᔕ↪䈮㑐䈜䉎⎇ⓥ㩷. శᾖ኿߿ pH ᄌൻ╬ߩᄖㇱೝỗ߿㉂⚛ߦࠃࠆછᗧߩ⠡⸶ᓟୃ㘼ߦᔕߓߡ‫ߩߘޔ‬ᯏ⢻߇ᵴᕈൻน⢻ߥ ࠲ࡦࡄࠢ⾰ߩੱᎿ⊛᭴▽ߪ‫⾰ࠢࡄࡦ࠲ޔ‬Ꮏቇ߿࠽ࡁࡃࠗࠝ࠹ࠢࡁࡠࠫ࡯ಽ㊁ߦ߅ߌࠆ㊀ⷐߥ⎇ⓥ⺖㗴 ߩ৻ߟߢ޽ࠆ‫ߥ߁ࠃߩߘޔߚ߹ޕ‬ᄖㇱೝỗᔕ╵ဳ࠲ࡦࡄࠢ⾰ߪ‫⚦ޔ‬⢩ᯏ⢻߿↢૕ᯏ⢻㑐ㅪ᧚ᢱߩ․ᕈ ࠍᤨⓨ㑆․⇣⊛ߦ೙ᓮน⢻ߥੱᎿಽሶࠬࠗ࠶࠴ߣߒߡᔕ↪߇ᦼᓙߐࠇࠆ‫ޕ‬ᚒ‫ޔߪޘ‬ಽഀဳ࠲ࡦࡄࠢ⾰ ߣੱᎿߩೝỗᔕ╵ㇱ૏ࠍ⚵ߺวࠊߖࠆߎߣߢ‫ޔ‬છᗧߩᄖㇱೝỗߦࠃߞߡᵴᕈൻน⢻ߥᯏ⢻ᕈ࠲ࡦࡄࠢ ⾰ߩ㐿⊒ࠍ⋡ᜰߒ‫⎇ޔ‬ⓥࠍዷ㐿ߒߡ޿ࠆ‫੹ޕ‬ᐕᐲߪ‫ޔ‬ಽഀဳ✛⦡Ⱟశ࠲ࡦࡄࠢ⾰ (Split GFP) ਛߦ㕖ᄤ ὼဳశ෻ᔕᕈㇱ૏߿ੱᎿᩭ㉄ߦട߃‫ޔ‬᭽‫ߥޘ‬㉂⚛෻ᔕᔕ╵ㇱ૏ࠍว⋡⊛⊛ߦⶄวൻߔࠆߎߣߦࠃߞߡ‫ޔ‬ ․ቯߩᄖㇱೝỗᷝടᤨ߿‫ ࠻࠶ࠥ࡯࠲ޔ‬RNA ޽ࠆ޿ߪ㉂⚛ሽ࿷ਅߦ߅޿ߡⰯశᒝᐲߩჇടࠍ␜ߔ GFP ᄌ⇣૕ߩ⸳⸘ࠍ⹜ߺߚ (࿑ 4)‫ޔߦࠄߐޕ‬᭴▽ߒߚಽഀဳ GFP ᄌ⇣૕ࠍ೑↪ߒߡ‫ޔ‬GFP Ⱟశᒝᐲᄌൻ ࠍᜰᮡߣߒߚ㜞ᗵᐲ↢૕ಽሶᬌ಴ࠪࠬ࠹ࡓߩ᭴▽ߣ᧚ᢱᔕ↪ࠍ⋡ᜰߒߚ‫ޕ‬ శ೙ᓮน⢻ߥಽഀဳ)(2ᄌ⇣૕ߩ᭴▽ ․ቯᵄ㐳ߩశᾖ኿ߦࠃߞߡᵴᕈൻߔࠆߎߣ߇น⢻ߥಽ ഀဳ GFP ߣߒߡ‫ޔ‬ಽഀဳ GFP ߩ C ᧃᢿ (17 ᱷၮ) ਛߩ Glu ᱷၮ஥㎮ߦኻߒ‫ޔ‬శಾᢿᕈቭ⢻ၮߢ ޽ࠆ 7-diethylamino-4-hydroxymethylcoumarin (DEAC) ࠍዉ౉ߒߚ‫ޕ‬శᾖ኿೨ߦߪ‫┙ޔ‬૕㓚ኂߦࠃߞߡ‫ޔ‬ ಽഀဳ GFP ߩ N- ߅ࠃ߮ C-ᧃ┵ࡈ࡜ࠣࡔࡦ࠻㑆ߩౣ⚿วߪ‫߷߶ޔ‬ቢోߦᛥ೙ߐࠇߚ‫৻ޕ‬ᣇ‫ޔ‬ᵄ㐳 400 nm એ਄ߩశᾖ኿ࠍⴕߞߚ႐ว‫ޔ‬DEAC ၮߩㅦ߿߆ߥಾᢿߣߣ߽ߦ GFP ߦ↱᧪ߔࠆᵄ㐳 510 nm Ⱟశ ᒝᐲߩჇടࠍ᷹ⷰ಴᧪ߚ‫ޕ‬એ਄ߩ⚿ᨐߪ‫ޔ‬ಽഀဳ࠲ࡦࡄࠢ⾰ߣశ෻ᔕᕈၮߩⶄวൻ߇‫ޔ‬ᄖㇱೝỗᔕ╵ ဳ࠲ࡦࡄࠢ⾰ߩ⸳⸘ߦ᦭ലߢ޽ࠆߎߣࠍ␜ໂߔࠆ‫ޕ‬ છᗧߩ㉂⚛ߦࠃߞߡᵴᕈൻߐࠇࠆಽഀဳ)(2ᄌ⇣૕ߩ᭴▽ ಽഀဳ࠲ࡦࡄࠢ⾰ਛߦࠞࠬࡄ࡯࠯ ၮ⾰㈩೉ࠍⶄวൻߔࠆߎߣߦࠃߞߡ‫․ޔ‬ቯߩࠞࠬࡄ࡯࠯ሽ࿷ਅߦ߅޿ߡⰯశࠍ␜ߔ GFP ᄌ⇣૕ߩ⸳⸘ ࠍ⹜ߺߚ‫੹ޕ‬࿁ߩታ㛎ߢߪ‫ ޔ‬C ᧃࡈ࡜ࠣࡔࡦ࠻ߩ N ᧃ┵஥ߦ‫ߩ࠯࡯ࡄࠬࠞޔ‬ၮ⾰㈩೉ࠍઃടߒߚ‫ޕ‬ ߐࠄߦ‫ ߩ࠼࠴ࡊࡍޔ‬N-߅ࠃ߮ C-ᧃ┵ࠍ‫⚿࡞࠹࡯ࠛࠝ࠴ޔ‬วࠍ੺ߒߡ᨞ᯅߔࠆߎߣߢ‫ޔ‬Ⅳ⁁ࡍࡊ࠴࠼ ࠍ᭴▽ߒߚ‫ޕ‬ಽഀဳ GFP ߦࠞࠬࡄ࡯࠯ࠍᷝടߒߚ႐ว‫࠼࠴ࡊࡍޔ‬ਛߩၮ⾰㈩೉ㇱ૏߇․⇣⊛ߦಾᢿߐ ࠇ‫ޔ‬GFP N ᧃᢿ ߣߩౣ⚿ว߅ࠃ߮࡝ࡈࠜ࡯࡞࠺ࠖࡦࠣല₸߇ะ਄ߔࠆߣหᤨߦ‫ޔ‬㗼⪺ߥⰯశᒝᐲߩ Ⴧട߇⹺߼ࠄࠇߚ‫ߩߎޕ‬ೋᦼ࠺࡯࠲࡯ߦၮߠ߈‫⃻ޔ‬࿷᭴▽ߒߚಽഀဳ GFP ᄌ⇣૕ࠍ೑↪ߒߡ‫ޔ‬GFP Ⱟ శᒝᐲᄌൻࠍᜰᮡߣߒߚࡊࡠ࠹ࠕ࡯࠯ᵴᕈᬌ಴ࠪࠬ࠹ࡓߩ᭴▽ࠍᬌ⸛ߒߡ޿ࠆ‫ޕ‬ . Figure 4. ಽഀဳ࠲ࡦࡄࠢ⾰ࠍ೑↪ߒߚᄖㇱೝỗᔕ╵ဳᯏ⢻ᕈ࠲ࡦࡄࠢ⾰ߩ᭴▽. 6.

(10) 7. ‫⎇ޣ‬ⓥᵴേႎ๔‫ޤ‬. 研究活動報告. ㊄ዻᯏ⢻⸳⸘⎇ⓥಽ㊁㧔㨪㧕. ᝼㧦⬰ ቟㇌ ᢎ ಎ ᢎ ᝼㧦੉ጟ ⡡ ഥ ᢎ㧦⮮↰િዏ ᛛ ቭ㧦ᄢᯅ ⻀ ᄢቇ 㒮↢㧦D2㧦㆙⮮ᚑノ‫ޔ‬M2㧦␹ጊᦸ‫᧛ᧁޔ‬⍮ผ‫ޔ‬ጊญஜ๋‫ޔ‬ M1㧦⩵࿾⌀৻‫ޔ‬ᨰᧁ␭੺‫ޔ‬⍹ේ↰ᐘᄥ‫ޔ‬ጤ↰ᢘผ‫ޔ‬ GCOE ࡈࠚࡠ࡯㧦Can Cui ᧄ⎇ⓥಽ㊁ߢߪ‫ޔ‬Kristnau Biswas ߪᧄ࿖ශᐲߩᄢቇߩᢎ⡯ߦઃߊߚ߼ߦ‫ޔ‬ቇⴚᝄ⥝ળߩᅑബ⎇ ⓥຬߣߒߡ㧡᦬ࠍ߽ߞߡㅌ⡯ߒߚ‫ޕ‬㧝᦬ߦ GCOE ߩࡈࠚࡠ࡯ߣߒߡ‫ޔ‬Can Cui ᳁߇⎇ⓥቶߦടࠊ ߞߚ‫ޕ‬㧠᦬ࠃࠅඳ჻ᓟᦼ⺖⒟ߦᓳᏫߒߚ㆙⮮ᚑノߩ߶߆‫⎇ޔ‬ⓥቶ㈩ዻࠍᏗᦸߒߚᄢቇ㒮೨ᦼ㧞ᐕ ⺖⒟⩵࿾⌀৻‫ޔ‬ᨰᧁ␭੺‫ޔ‬⍹ේ↰ᐘᄥ‫ޔ‬ጤ↰ᢘผߩ 4 ੱࠍ⬰⎇ⓥቶߣߒߡฃߌ౉ࠇ‫⎇ޔ‬ⓥᵴേߔ ࠆߎߣߣߒߚ‫⎇ᧄޕ‬ⓥಽ㊁ߦ߅ߌࠆ 2008 ᐕߩᵴേࠍ᭎᜝ߔࠆߣએਅߩㅢࠅߢ޽ࠆ‫ޕ‬ 㧝㧕Ḱ⚿᥏ߩ቟ቯᕈ ᤓᐕᐲߢߪ‫ޔ‬%F4' 4'Ꮧ࿯㘃㊄ዻ ߦኻߒߡ /I ߩ %F ⟎឵ࠍⴕ߁ߎߣߦࠃࠅ‫ޔ‬ᄙߊߩ቟ቯߥḰ⚿᥏ࠍ ᒻᚑߔࠆߎߣ߇᣿ࠄ߆ߦߒߚ‫ޕ‬%F4' ߦኻߒߡ %F ࠍ /I ߢ⟎឵ߔࠆߎߣߦࠃߞߡ‫ޔ‬%F/I;%F/I&[ ߅ࠃ߮ %F/I)F ࠍߪߓ߼ᄙߊߩ቟ቯߥḰ⚿᥏߇ᒻᚑߐࠇࠆߎߣ߇⏕⹺ߐࠇ‫ޔ‬Ḱ⚿᥏ߩᒻᚑߦᔅⷐߥ /I ߩ⟎឵㊂ߪ 4' ߦࠃߞߡ CV߿ CVߦߥࠆߎߣ߇ಽ߆ߞߚ‫੹ޕ‬ᐕᐲߢߪ‫ ޔ‬ㄭૃ⚿᥏ߦ߅ߌࠆ /I ⟎឵ߩ㓙‫ޔ‬/I ߇ఝవߦභ߼ࠆࠨࠗ࠻ࠍන⚿᥏᭴ㅧ⸃ᨆ߆ࠄ⸃᣿ߒߚ‫ޕ‬/I ߩᷝടߦࠃߞߡ‫ࡓ࠳ࡦ࡜ޔ‬ ߢߪߥߊ‫․ࠆ޽ޔ‬ቯߥ %F ߩࠨࠗ࠻߆ࠄ㗅⇟ߦ⟎឵ߒߡ޿ߊߎߣ߇᣿ࠄ߆ߦߥߞߚ‫߫߃଀ޕ‬㧘ࠢ࡜ࠬ࠲ ࡯⧌ߦ޽ࠆ㧠ߟߩ %F ේሶߪᦨೋߦ⟎឵ߐࠇߡ޿ࠆ‫ޔߚ߹ޕ‬/I ߩᷝടߦࠃߞߡḰ⚿᥏߇ᒻᚑߐࠇߚ 4' ߩේሶඨᓘߪ޽ࠆ▸࿐ౝߦ㒢ࠄࠇߡ޿ࠆ‫ߩࠄࠇߎޕ‬Ḱ⚿᥏ߪ଀ᄖߥߊ‫ޔ‬4' ߩ฽᦭㊂߇⚂ CVߦߣߤ ߹ࠅ‫ޔ‬%F ♽߿ +P#I ♽ߩḰ⚿᥏ߩ 4' ޯCV ㊂ߦᲧߴߡ㧘⚂㧠CV߽ዋߥߊߥߞߡ޿ࠆ‫ߣߎߩߎޕ‬ ߪ‫ޔ‬%F/I4' Ḱ⚿᥏߇ %F;D Ḱ⚿᥏ߣหߓ᭴ㅧࠍ᦭ߔࠆߣ઒ቯߒߚ႐ว‫ޔ‬/I ߪ৻ㇱߩ 4' ࠨࠗ࠻ࠍ⟎ ឵ߔࠆߎߣߣផ᷹ߐࠇࠆ‫ߩࠄࠇߎޕ‬᭴ㅧ⸃᣿ߦࠃߞߡḰ⚿᥏ߩ᭴ㅧ቟ቯൻᯏ᭴ߦ㊀ⷐߥᚻ߆߆ࠅ߇ᓧ ࠄࠇࠆ⠨߃ࠄࠇࠆ‫ޔߦࠄߐޕ‬%F;D ߿ %F%C ߆ࠄ᜛ᒛߒߚ +P#I4' ♽ࠍߐࠄߦᐢߍߡ‫ޔ‬GC ଔ㔚ሶỚ ᐲ ࠍၮḰߦ‫ޔ‬#W5P4' ߅ࠃ߮ #W)G4' ♽ߦ߅޿ߡᄙߊߩㄭૃ⚿᥏ߩᒻᚑࠍ⏕⹺ߒߚ‫ޕ‬ 㧞㧕Ḱ⚿᥏ಽᢔ /I ว㊄ߩ૞⵾ <P/I<T Ḱ⚿᥏ࠍ೨㚟‛⾰ߣߒߡ૞⵾ߒߚ /I ว㊄㍌ㅧ᧚ ߪ‫ޔ‬/I ၮ࿾ߦᓸ⚦ߥ <P<T ㊄ዻ㑆ൻว‛ ߣᏪ⁁ߩ <P/I ㊄ዻ㑆ൻว‛߇ಽᢔߒߚ⚵❱ࠍ᦭ߒߚ‫ޕ‬/I ว㊄ߩࡃ࡞ࠢൻ߅ࠃ߮ဋ⾰ൻࠍ⋡⊛ߣߒߡ ☳ᧃಃ㊄ᴺߦ⌕⋡ߒ‫ޔ‬Ḱ⚿᥏ᓸ☳ᧃ㧔ǴO ⒟ᐲ㧕ࠍ↪޿ߡ᛼಴᧚ࠍ૞⵾ߒߚ‫ޕ‬૞⵾᧦ઙߪ᛼಴᷷ᐲ‫ޔ‬ ᛼಴Ყ‫ޔ‬᛼಴ㅦᐲߘࠇߙࠇ ͠‫ޔ‬㧦 ߘߒߡ OOOKP ߢⴕߞߚ‫ޕ‬૞⵾ߒߚ᛼಴᧚ߦߟ޿ߡ㔚ሶࡊ ࡠ࡯ࡉࡑࠗࠢࡠࠕ࠽࡜ࠗࠩ‫ޔ‬: ✢࿁᛬ⵝ⟎߅ࠃ߮ㅘㆊဳ㔚ሶ㗼ᓸ㏜ࠍ↪޿ߡ⚵❱ⷰኤߣ⋧ߩ⚵ᚑಽᨆࠍ ⴕߞߚ⚿ᨐ‫ޔ‬᛼಴᧚ߪ /I ၮ࿾ߦḰ⚿᥏߇⋥ធಽᢔߐࠇߚ⚵❱ࠍ␜ߒߡ߅ࠅ‫ޔ‬೨ㅀߩḰ⚿᥏ࠍ೨㚟‛⾰ ߣߒߡ૞⵾ߒߚ /I ว㊄ߣߪ⇣ߥࠆ߽ߩߢ޽ߞߚ‫ޕ‬Ḱ⚿᥏ಽᢔ᛼಴᧚ߩቶ᷷ߦ߅ߌࠆᒁᒛᒝᐲ߅ࠃ߮ ⠴ജߪߘࠇߙࠇ㨪/2C‫ޔ‬㨪/2C ࠍ␜ߒ‫ߪߢ͠ޔ‬ᒁᒛᒝᐲ㨪/2C‫ޔ‬⠴ജ㨪/2C ࠍ␜ߒߚ‫ޕ‬᛼಴᧚ߩᲣ⋧ߩ☸ᓘߪ߅ࠃߘ ǴO ߢ޽ࠅ‫ޔ‬ห⒟ᐲߩ☸ᓘࠍ߽ߟ⚐ࡑࠣࡀࠪ࠙ࡓ᛼಴᧚ߩ ⹜㛎⚿ᨐߣᲧセߔࠆߣ․ߦ ᐲߦ߅ߌࠆᒁᒛ⹜㛎ߦ߅޿ߡḰ⚿᥏ಽᢔߩലᨐ߇㗼⪺ߢ޽ߞߚ‫ޕ‬ 㧟㧕Ḱ⚿᥏ߣㄭૃ⚿᥏ߩ⚿᥏ᚑ㐳 %F;D ♽Ḱ⚿᥏ߪ㧞ర♽ߣ޿߁න⚐ߐߢ᭴ㅧ⸃ᨆߩᩰᅢߥኻ⽎ߣߒߡ↪޿ࠄࠇߚ߇‫‛ޔ‬ᕈ᷹ቯ߿⴫㕙 ⑼ቇߩ⎇ⓥߢߪ %F ߩើ⊒ᕈ߇໧㗴ߦߥߞߡ޿ࠆ‫ߩߘޔߡߞ߇ߚߒޕ‬ઍᦧߣߒߡ +P#I;D ♽߇↪޿ࠄ ࠇߡ޿ࠆ‫⃻ޕ‬࿷߹ߢ‫ޔ‬+P#I;D ♽ߩනḰ⚿᥏ߩ૞⵾ߦ߅޿ߡᚑഞߒߡ޿ࠆ߇‫ޔ‬లಽߥᄢ߈ߥ߅ࠃ߮ౣ ⃻ᕈ߇ᓧࠄࠇߥ߆ߞߚߚ߼‫੹ޔ‬ᐕᐲߦ߅޿ߡߐࠄߦ⹦⚦ߦᬌ⸛ߒߚ‫ޔߚ߹ޕ‬᭴ㅧ߿⻉‛ᕈߦ߅޿ߡḰ ⚿᥏ߣߩᲧセߣߒߡㄭૃ⚿᥏ߩᄢ߈ߥන⚿᥏߇㊀ⷐߢ޽ࠆߚ߼‫ߩߘޔ‬૞⵾߽⹜ߺߚ‫⚿ߩߘޕ‬ᨐ‫ޔ‬૞⵾.

(11) 研究活動報告. ᧦ઙࠍ⏕┙ߒߚߣߣ߽ߦ࿑㧝ߦ␜ߔࠃ߁ߦ EO ೨ᓟߩḰ⚿᥏߅ࠃ߮ޯ㧡OO ߩ ㄭૃ⚿᥏ࠍ૞⵾ߔࠆ ߎߣ߇ߢ߈ߚ‫ޕ‬Ḱ⚿᥏ߦߪ $TKFIOCP ᴺߣࡈ࡜࠶ࠢࠬߩਔᣇ߇ㆡ↪ߢ߈ߚߦኻߒߡ‫ ޔ‬ㄭૃ⚿᥏ߦߪ ࡈ࡜࠶ࠢࠬᴺߩߺ᦭ലߢ޽ࠆ‫ࠬࠢ࡜࠶ࡈޔߚ߹ޕ‬ᴺߢ૞⵾ߒߚḰ⚿᥏ߣㄭૃ⚿᥏ߩᒻᘒ߆ࠄߘࠇߙࠇ 㧡࿁ኻ⒓㕙ߣ 㕙߇቟ቯߢ޽ࠆߎߣ߇ಽ߆ߞߚ‫⃻ޕ‬࿷‫ޔ‬᭴ㅧࡕ࠺࡞ߦ߅ߌࠆේሶኒᐲߩಽᏓ߆ࠄ‫ޔ‬ ⚿᥏㕙ߩ቟ቯᕈࠍᬌ⸛ߔࠆߣߣ߽ߦ‫⹜ߩࠄࠇߎޔ‬ᢱࠍ↪޿ߚ⴫㕙⎇ⓥࠍㅴ߼ߡ޿ࠆ‫ޕ‬ ࿑㧝 ࡈ࡜࠶ࠢࠬᴺߢ૞⵾ߒߚḰ⚿᥏ C ߣ ㄭૃ⚿᥏ E ߅ࠃ߮ߘࠇߙࠇ 㧡࿁ኻ⒓ D ߣ F ᣇะ߆ࠄ᠟ߞߚ⢛㕙 : ✢࡜࠙ࠛ࿁᛬ࡄ࠲࡯ࡦ 㧠㧕ੑర♽ࡐ࡯࡜ࠬว㊄⸅ᇦߩᣂⷙ⺞⵾ᴺߩ㐿⊒㧔ᶐẃว㊄ൻᴺ㧕 ᧄᐕᐲߩ࠻ࡇ࠶ࠢࠬߩ㧝ߟߣߒߡߪ‫ޔ‬㕖ᜂᜬဳ㧞ర♽ࡐ࡯࡜ࠬว㊄⸅ᇦߩᣂߒ޿⺞⵾ࡊࡠ࠮ࠬ㧔ᶐ ẃว㊄ൻᴺ㧕ࠍ⏕┙ߒߚߎߣߢ޽ࠆ‫⸅ဳ࡯ࡀ࡜ޕ‬ᇦߦઍ⴫ߐࠇࠆࠃ߁ߦ㕖ᜂᜬဳࡐ࡯࡜ࠬ㊄ዻ⸅ᇦߪ ฎߊ߆ࠄ⍮ࠄࠇߡ޿ࠆ‫ߪࠄࠇߎޔߒ߆ߒޕ‬න৻㊄ዻ♽ߢ޽ࠆߎߣ߆ࠄ․ߦᾲ⊛቟ቯᕈߦਲߒߊ↪ㅜ߇ 㒢ቯߐࠇߡ޿ࠆ‫৻ޕ‬ᣇ‫ޔ‬ว㊄ߪ㊄ዻห჻ߩ⋧੕૞↪㧔ว㊄ൻലᨐ㧕ࠍ᦭ലߦᵴ↪ߢ߈ᵴᕈ‫ޔ‬ㆬᛯᕈ‫ޔ‬ ⠴ᾲᕈߥߤߩᯏ⢻ะ਄߇ᦼᓙߢ߈ࠆߎߣ߆ࠄ‫ࠬ࡜࡯ࡐޔ‬ว㊄⸅ᇦߦ⥝๧߇ᜬߚࠇߡ߈ߚ‫ޕ‬ ᧄ⎇ⓥߢߪ‫ޔ‬Al ၮ㊄ዻ㑆ൻว‛㧔Al3Ni, ǫAl-Pd ว㊄㧕ࠍ 20wt%NaOH ᳓ṁᶧߦࠃࠅㆬᛯ⊛ṁ಴ ಣℂ㧔࡝࡯࠴ࡦࠣ㧕ߐߖߡᓧߚࡐ࡯࡜ࠬ㊄ዻ⸅ᇦߦ╙ੑ㊄ዻర⚛ࠍ฽߻㊄ዻႮ᳓ṁᶧ(ZnCl2 etc.)ࠍ฽ᶐ ᜂᜬᓟ‫ޔ‬ว㊄ൻಣℂ㧔H2 ਛ, 500oC for 12h㧕ࠍᣉߔᚻᴺߦࠃࠅ‫ޔ‬㕖ᜂᜬဳࡐ࡯࡜ࠬว㊄⸅ᇦࠍ⺞⵾ߒߘ ߩว㊄ൻലᨐߦߟ޿ߡᬌ⸛ߒߚ‫ޕ‬ ⺞⵾ߒߚ 0K<P‫ޔ‬2F<P ♽ว㊄⸅ᇦߩ :4&⚦ሹಽᏓ‫࡞࡯ࡁ࠲ࡔޔ‬᳓⫳᳇ᡷ⾰㧔54/㧕෻ᔕߩ⚿ᨐࠍ ࿑ ߥࠄ߮ߦ⴫㧝ߦ␜ߔ‫⚿ߩࠄࠇߎޕ‬ᨐ߆ࠄ‫ޔ‬0K<P ♽2F<P ♽ߣ߽ߦ‫ࠆߔ᦭ࠍࠕࡐ࠰ࡔޔ‬ว㊄߇ᧄ ᚻᴺ㧔ᶐẃว㊄ൻᴺ㧕ߦࠃࠅ૞⵾ߢ߈ࠆߎߣ߇ࠊ߆ߞߚ‫⸅ߩࠄࠇߎޕ‬ᇦࠍ↪޿ߡࡔ࠲ࡁ࡯࡞᳓⫳᳇ᡷ ⾰෻ᔕ㧔%*1* *1ψ%1 *㧕ࠍⴕߞߚ⚿ᨐ‫ޔ‬0K ߣ 0K<P ว㊄‫ޔ‬2F ߣ 2F<P ว㊄ߣߢߪ↢ᚑ‛ߩ ㆬᛯ₸ߦᄢ߈ߥᏅ߇⷗ࠄࠇ‫ޔ‬᣿⏕ߥว㊄ൻലᨐ߇⏕⹺ߢ߈ߚ㧔⴫ ‫⚿ߩߎޕ‬ᨐߪ‫<ޔ‬P ߣߩว㊄ൻߦࠃ ࠅ 0K2F ߩ㔚ሶ⁁ᘒ߇ᄌൻߒ‫ޔ‬೽෻ᔕ߇⪺ߒߊᛥ೙ߐࠇߚߣ⠨߃ࠄࠇࠆ‫ޕ‬ታ㓙‫ࡒ࡞ࠚࡈޔ‬Ḱ૏ '( ߦ ኻߒ 0K<P2F<P ว㊄ߩଔ㔚ሶᏪ㧔F ࡃࡦ࠼㧕ߩ਄┵૏⟎ߪ 0K2F ߩ႐วߦᲧߴࠆߣࠃࠅૐߊߥࠆߎ ߣ߆ࠄ‫ޔߦ․ޔ‬%1 ߩๆ⌕࡮⸃㔌߇ᛥ߃ࠄࠇࡔ࠲ࡀ࡯࡚ࠪࡦ߇ㅴⴕߒߥ޿ߣ⸃㉼ߢ߈ࠆ㧔0K ♽ߩ႐ว㧕 ‫ޕ‬ ߹ߚ‫ᧄޔ‬ᚻᴺߢᓧࠄࠇߚ‫ࠬ࡜࡯ࡐޔ‬ว㊄⸅ᇦߪ‫ޔ‬ᓥ᧪ߩ࠽ࡁ☸ሶ⸅ᇦߣ⇣ߥࠅᾲ቟ቯᕈߦఝࠇߡ޿ࠆ ߎߣ߇ࠊ߆ߞߚ‫ޕ‬. a. b. c. d. 8.

(12) 9. 研究活動報告㩷. 㩷㪈㪌㪇. 㪉㪇㪇㪇. ⴫㧝 ฦว㊄ߩ SRM ㆬᛯ₸Ყセ Sample Selectivity (%)a) ٧ 㪈㪉㪇㪈㪍㪇㪇 ٧ ً CO CO2 CH4 ٧ after alloying Ni 2.8 41.0 56.2 ٌ ٌ Ni 㪐㪇㪈㪉㪇㪇 ٌ NiZn 97.4 1.9 0.7 before alloying Pd 97.1 0.7 2.2 Pd-Zn system 㪍㪇 ‫ع‬ 㪏㪇㪇 PdZn 0.5 99.5 0 NiZn ‫ ع‬PdZn (L1 ) (33 m /g) a) ‫ع‬ ‫ ع ع ع‬㪊㪇 ෻ᔕ᷷ᐲ㧦360 oC Pd (31 m /g) 㪋㪇㪇 after alloying ‫غ‬ ‫ غ‬Pd PdZn ‫غ‬ (23 m /g) before alloying 㪇 Ni (45 m /g) 㪇㪊㪇㪋㪇㪌㪇㪍㪇㪌㪇㪈㪈㪇 2 Theta / deg. rp / nm ࿑ 0K<P2F<P ♽ว㊄ߩ :4& ߥࠄ߮ߦ⚦ሹಽᏓ 㧡㧕᳓⚛ൻൻว‛ࠍ↪޿ߚᣂⷙ⸅ᇦ㐿ᜏ ㄭᐕ‫ޔ‬㊄ዻ᧚ᢱߦ᳓⚛ࠍๆ⬿ߐߖࠆ⋡⊛ߪ‫ޔ‬ਥߦ᳓⚛⾂⬿↪᧚ᢱߩ㐿⊒‫ޔ‬᳓⚛ߩ♖⵾߿⚵❱ߩᓸ⚦ ൻߢ޽ߞߚ‫৻ޕ‬ᣇ‫⸅ޔ‬ᇦൻቇߩಽ㊁ߢߪ‫⴫ޔ‬㕙࡮ๆ⬿᳓⚛ࠍ὇ൻ᳓⚛㘃ߩ᳓⚛ൻ෻ᔕߦ↪޿ࠆߣ޿߁ ႎ๔ߪ޽ࠆ߽ߩߩ‫ޔ‬᳓⚛ๆ⬿ಣℂ߿᳓⚛ൻൻว‛ߘߩ߽ߩࠍ⸅ᇦ᧚ᢱߦ↪޿ߚႎ๔଀ߪ߶ߣࠎߤߥ߆ ߞߚ‫ޕ‬᳓⚛ࠍๆ⬿ߔࠆߣ᭽‫ߥޘ‬᭴ㅧ࡮‛ᕈ࡮⚵❱ߦᄌൻࠍਈ߃ࠆߩߢ‫ޔ‬᳓⚛ๆ⬿ߪᣂⷙߥ⸅ᇦ᧚ᢱߩ ⺞⵾ᴺߣߒߡ߽᦭ലߢ޽ࠆߣ⠨߃ࠄࠇࠆ‫⎇ᧄޕ‬ⓥߪ‫ޔ‬᳓⚛ๆ⬿ಣℂ߹ߚߪ᳓⚛ൻൻว‛ࠍ⸅ᇦ᧚ᢱ࡮ ⺞⵾ߣߒߡ೑↪ߔࠆ߽ߩߢ޽ࠅ‫ޔ‬㊄ዻቇߣ⸅ᇦൻቇࠍⲢวߐߖߚ߹ߞߚߊᣂߒ޿⎇ⓥߢ޽ࠆ‫ޕ‬ after absorption (a) AuZr 3 before and after hydrogen absorption 2CO + O 2 2CO 2 100 before 80 after 60 40 (b) before absorption 20 0 0 50 100 150 200 250 300 o Tem perature / C ࿑ 3. ᳓⚛ๆ⬿೨ᓟ AuZr3 ߩ CO㉄ൻ෻ᔕߦ߅ߌࠆ CO ォൻ₸ ࿑ 4. ᳓⚛ๆ⬿೨ᓟߦ߅ߌࠆ AuZr3 ߩ CO ㉄ൻ෻ᔕᓟߩ SEM ௝ ᧄ⎇ⓥߢߪ᳓⚛ๆ⬿ࠍ⌀ⓨ⁁ᘒ߆ࠄQ%࡮/2Cߩ᳓⚛࿶ࠍශടߒߡⴕߞߡ޿ࠆ‫ޕ‬#W<T♽㊄ዻ 㑆ൻว‛ߩ᳓⚛ๆ⬿㊂ߪ<TỚᐲߦଐሽߒߡჇടߒ‫ޔ‬᭴ㅧଐሽᕈ߇ૐ޿ߎߣࠍ᣿ࠄ߆ߦߒߚ‫ޔߚ߹ޕ‬ #WỚᐲ߇Ⴧടߔࠆߣ᳓⚛ๆ⬿᷷ᐲ߇ૐਅߔࠆߎߣ߽ಽ߆ߞߚ‫ޕ‬#<T ##W#I%W ߩ᳓⚛ๆ⬿㊂ ߦ߅޿ߡߪ#W<T߇⪺ߒߊዊߐߊ‫ޔ‬#I<T‫ޔ‬%W<Tߩ㗅ߦჇ߃ࠆߎߣ߇ಽ߆ߞߚ‫ޔߪࠇߎޕ‬#W<Tߢߪ ᳓⚛ߪ࿕ṁߔࠆߩߦኻߒ‫ޔ‬#I<Tߣ %W<T ߪ᳓⚛ࠍๆ⬿ߔࠆߣਇဋൻ෻ᔕࠍ⿠ߎߒ <T* ߣ #I‫ޔ‬%W ߳ߣ ಽ⸃ߔࠆߚ߼ߢ޽ࠆ‫ޕ‬᳓⚛ๆ⬿ #W<T ߩ㉄ൻ᷷ᐲߪ Q%એ਄ૐਅߔࠆߚ߼‫ޔ‬%1㉄ൻ෻ᔕߦ߅޿ߡ߽ ૐ᷷߆ࠄ෻ᔕ߇ㅴⴕߒ‫ޔ‬᳓⚛ࠍๆ⬿ߐߖߥ޿ #W<Tࠃࠅૐ᷷ߢ%1ォൻ₸߇߶߷ ߣߥߞߚ ࿑ ‫ޕ‬ ً. NiZn (L10) Ni-Zn alloy. 0. 㩷. ً. 㩷 dVp/d(logrp). Intensity / a. u.. Ni-Zn system. 2. 2. 2. 2. CO conversion / %. QuickTimeý Ç² TIFF (Uncompressed) êLí£ÉvÉçÉOÉâÉÄ Ç ™Ç ± Ç ÃÉ É s N `É É Ç ¾å © ÇÇ ÈÇ ž ½… Ç Ç ÕK ï ó v Ç - Å ÇB.

(13) 研究活動報告. 10. ෻ᔕᓟߩ⴫㕙Ⓧ߽೨⠪ߪᩴએ਄Ⴧടߒߚ߇‫ޔ‬ᓟ⠪ߪߐ߶ߤᄌൻߒߥ߆ߞߚ‫ޕ‬࿑ߪ᳓⚛ๆ⬿᦭ήߩ #W<Tߦ߅ߌࠆ෻ᔕᓟߩ5'/௝ߢ޽ࠆ‫ޕ‬೨⠪ ࿑ C ߩᣇ߇#W☸ሶ߇⚦߆ߊಽᢔߒߡ޿ࠆߎߣ ߇ಽ߆ߞߚ‫ ޕ‬#I%W <T ߦ߅޿ߡ߽᳓⚛ๆ⬿ߐߖࠆߣࠃࠅૐ᷷߆ࠄ %1㉄ൻ෻ᔕ߇ㅴⴕߒߚ‫ࠇߎޕ‬ ߪ᳓⚛ๆ⬿ߦࠃࠅ↢ߓߚ #I‫ޔ‬%W࠽ࡁ☸ሶ߇⴫㕙ߦᨆ಴ߒ‫ޔ‬ᵴᕈὐߣߥߞߡ޿ࠆߚ߼ߢ޽ࠆ‫ޕ‬ 䋶㪀㩷 ᱜ䋲䋰㕙૕Ḱ⚿᥏䈮ኻ䈜䉎䉪䊤䉴䉺䈱ⓨ㑆లႯ䊝䊂䊦㩷 ᱜ㧞㧜㕙૕Ḱ⚿᥏ߩ᭴ㅧߪ‫ޔ‬㜞ኒᐲߥේሶ㓸ว૕ߢ޽ࠆᱜ㧞㧜㕙૕⊛ࠢ࡜ࠬ࠲߇Ḱ๟ᦼ⊛ߦⓨ㑆ࠍ లႯߒߚ᭴ㅧߣℂ⸃ߐࠇߡ޿ࠆ‫ߩ࠲ࠬ࡜ࠢߥ⚦⹦ޔߒ߆ߒޕ‬ⓨ㑆㈩⟎߿ߘࠇ߇ታ⃻ߔࠆ᧦ઙߦ㑐ߒߡ ߪ‫ޔ‬ᄙߊߩ⇼໧߇ᱷߐࠇߡ޿ࠆ‫⎇ᧄޕ‬ⓥߢߪ‫ޔ‬ᱜ㪉㪇㕙૕Ḱ⚿᥏ߦ߅ߌࠆࠢ࡜ࠬ࠲ߩ㈩⟎ߦኻߔࠆᐞ૗ ቇ⊛ߥ⠨ኤࠍⴕߞߚ‫ޕ‬㩷 ߹ߕ‫ޔ‬ᢙቇ⊛ࠕ࡞ࠧ࡝࠭ࡓߦࠃࠆᣂߒ޿ᱜ㪉㪇㕙૕Ḱᩰሶߩ૞ᚑࠍⴕߞߚ‫ޕ‬૞ᚑᚻᴺߣߒߡ‫ޔ‬㪥㫀㫀㫑㪼㫂㫀㩷㩿㪉㪇㪇㪏㪀㩷 ߦࠃࠅ㪉ᰴరḰᩰሶߩ૞ᚑᴺߣߒߡ⠨᩺ߐࠇߚࡐࠗࡦ࠻ࠗࡦࡈ࡟࡯࡚ࠪࡦ࡞࡯࡞ࠍ‫ޔ‬ᱜ㪉㪇㕙૕Ḱ ᩰሶߩ૞ᚑߦᔕ↪ߒߚ‫ߩߎޕ‬ᚻᴺߢߪ‫ޔ‬ㆡᒰߥၮᧄࠢ࡜ࠬ࠲ࠍ಴⊒ὐߣߒ‫⥄ޔ‬Ꮖ⋧ૃᄌ឵ⷙೣߩ➅ࠅ ㄰ߒߦࠃߞߡḰᩰሶ߇↢ᚑߐࠇࠆ‫⎇ᧄޕ‬ⓥߢߪ‫ࠅࠃޔ‬ᄙ᭽ߥḰᩰሶࠍ૞ᚑߔࠆߚ߼ߦࡐࠗࡦ࠻ࠗࡦࡈ ࡟࡯࡚ࠪࡦ࡞࡯࡞ߩ᜛ᒛ߽ⴕߞߚ‫⚿ߩߘޕ‬ᨐ‫ޔ‬㪊ᰴరࡍࡦࡠ࡯࠭ᩰሶߥߤᓥ᧪߆ࠄ⦟ߊ⍮ࠄࠇߡ޿ࠆ߽ ߩߦട߃ߡ‫ޔ‬ᢙᄙߊߩᣂߒ޿Ḱᩰሶࠍ૞ᚑߔࠆߎߣ߇಴᧪ߚ‫ޕ‬૞ᚑߒߚ㪝ဳᱜ㪉㪇㕙૕Ḱᩰሶߩ଀ࠍ࿑㪍㩿㪸㪀 ߦ␜ߔ㧔㪌࿁ኻ⒓ゲߦု⋥ߥࠬ࡜ࠗࠬߩᛩᓇ࿑㧕‫ޕ‬㩷 ᰴߦ‫ޔ‬਄⸥ߩ⚿ᨐࠍ↪޿ߡᱜ㪉㪇㕙૕Ḱ⚿᥏ߦኻߔࠆℂᗐ⊛ߥࠢ࡜ࠬ࠲ߩⓨ㑆లႯ᭴ㅧࠍត⚝ߒߚ‫ޕ‬ 㪚㪻㪌㪅㪎㪰㪹ᱜ㧞㧜㕙૕Ḱ⚿᥏ߦ߅޿ߡ‫⪉ޔ‬ᒻ㧟㧜㕙૕ࠢ࡜ࠬ࠲߇ၮᧄ⊛ߥ᭴ㅧ࡙࠾࠶࠻ߢ޽ࠆߎߣ߇⍮ࠄ ࠇߡ޿ࠆ㩿㪫㪸㫂㪸㫂㫌㫉㪸㩷 ࠄ‫ޔ‬㪉㪇㪇㪎㪀‫ޔߪ࠲ࠬ࡜ࠢߩߎޕ‬㧞࿁ゲᣇะߦ⪉ᒻߩ㕙ࠍធߒߚ㪹㪄㫃㫀㫅㫂㪸㪾㪼‫ޔ‬෸߮‫ޔ‬㧟࿁ ゲᣇะߦࠢ࡜ࠬ࠲ห჻߇ㇱಽ⊛ߦ⽾౉ߒߚ㪺㪄㫃㫀㫅㫂㪸㪾㪼ߦࠃߞߡ੕޿ߦ⚿วߒ‫ࠢ࡯ࡢ࠻࠶ࡀߩ࠲ࠬ࡜ࠢޔ‬᭴ ㅧࠍ᭴ᚑߔࠆ‫ޕ‬࿑㪌㩿㪸㪀ߩḰᩰሶߢߪ‫ޔ‬ㄭធߔࠆᩰሶὐߩᄙߊ߇㩷㪹㪄෸߮㩷㪺㪄㫃㫀㫅㫂㪸㪾㪼ߦࠃߞߡ⚿߫ࠇࠆ߇‫ޔ‬ ᩰሶὐ߇ㆊኒߥߚ߼ߐࠄߦ⍴〒㔌ߩ㫃㫀㫅㫂㪸㪾㪼߇޽ࠆഀวߢ฽߹ࠇࠆ‫৻ޔߢߎߘޕ‬ㇱߩᩰሶὐߩ㈩⟎ࠍ⺞ ᢛ㧔ᶖ෰࡮ㅊട㧕ߒ‫ޔ‬⍴〒㔌ߩ㫃㫀㫅㫂㪸㪾㪼ࠍ฽߹ߥ޿ℂᗐ⊛ߥ㩷㪹㪄㪺㩷 ࡀ࠶࠻ࡢ࡯ࠢࠍត⚝ߒߚ‫ޕ‬࿑㪌㧔㪹㧕ߪ ᓧࠄࠇߚ᦭㒢ࠨࠗ࠭ߩ㪹㪄㪺㩷 ࡀ࠶࠻ࡢ࡯ࠢߢ޽ࠆ‫ޕ‬࿑㪌㧔㪺㧕ߪ‫ޔ‬㧔㨎㧕ߩᩰሶὐ਄ߦ⪉ᒻ㪊㪇㕙૕ࠍ㈩⟎ ߒߚ߽ߩߢ‫߇࠲ࠬ࡜ࠢޔ‬ⓨ㑆ࠍ㜞ኒᐲߦలႯߒߡ߅ࠅ‫ޔ‬ᱷߐࠇߚࠊߕ߆ߥ㓗㑆ߪ㍈ⷺ⪉ᒻ㪍㕙૕ߦࠃߞ ߡභ߼ࠄࠇࠆ‫ޕ‬Ḱ⚿᥏ߩℂᗐ⊛ߥ᭴ㅧࡕ࠺࡞ࠍ᭴▽ߔࠆߚ߼ߦ‫ޔ‬ή㒢ࠨࠗ࠭ߩℂᗐ⊛㩷㪹㪄㪺ࡀ࠶࠻ࡢ࡯ ࠢߩሽ࿷ࠍℂ⺰⊛ߦ␜ߖࠆ߆ߤ߁߆߇ࡐࠗࡦ࠻ߣߥࠆ‫ޕ‬㩷㩷. ࿑㧢 (a) ࡐࠗࡦ࠻ࠗࡦࡈ࡟࡯࡚ࠪࡦ࡞࡯࡞ߦࠃࠅ૞ᚑߒߚ ࿑ 5 (a) ࡐࠗࡦ࠻ࠗࡦࡈ࡟࡯࡚ࠪࡦ࡞࡯࡞ߦࠃࠅ૞ ᣂߒ޿ᱜ 20 㕙૕Ḱᩰሶ‫ޔ‬ (b) ℂᗐ⊛ߥ b-c ࡀ࠶࠻ࡢ࡯ࠢ‫(ޔ‬c) ᚑߒߚᣂߒ޿ᱜ 20 㕙૕Ḱᩰሶ‫(ޔ‬b) ℂᗐ⊛ߥ b-c ࡀ࠶ (b)ߦၮߠߊ⪉ᒻ 30 㕙૕ࠢ࡜ࠬ࠲ߩ㈩⟎‫ޕ‬ ࠻ࡢ࡯ࠢ‫(ޔ‬c) (b)ߦၮߠߊ⪉ᒻ 30 㕙૕ࠢ࡜ࠬ࠲ߩ㈩⟎ 㩷㩷.

(14) 11. 研究活動報告. 䋷㪀㩷䋲ᰴరḰ๟ᦼ䉺䉟䊥䊮䉫䈱৻⥸⊛↢ᚑᴺ䈱⠨᩺䈫ᣂ䈚䈇䉺䉟䊥䊮䉫䈱૞ᚑ㩷 ᓥ ᧪ ‫ ࠍ ࠣ ࡦ ࡝ ࠗ ࠲ ࠭ ࡯ ࡠ ࡦ ࡍ ޔ‬ઍ ⴫ ଀ ߣ ߔ ࠆ ዋ ᢙ ߩ Ḱ ๟ ᦼ ࠲ ࠗ ࡝ ࡦ ࠣ ߇ ‫ ޔ‬㪈㪇 ࿁ ኻ ⒓ Ḱ ⚿ ᥏ 㧔㪻㪄㪘㫃㪄㪥㫀㪄㪚㫆‫ޔ‬㪻㪄㪘㫃㪄㪚㫌㪄㪚㫆╬㧕ߦ߅ߌࠆේሶࠢ࡜ࠬ࠲ߩ㈩⟎ࠍ⸥ㅀߔࠆᢙℂࡕ࠺࡞ߣߒߡᐢߊ↪޿ࠄ ࠇߡ᧪ߚ‫⃻ޔߒ߆ߒޕ‬ታߩ㪈㪇࿁ኻ⒓Ḱ⚿᥏ߦ߅ߌࠆේሶࠢ࡜ࠬ࠲ߩ㈩⟎ߪ‫⚵ޔ‬ᚑ߿᷷ᐲߦࠃߞߡᄙ᭽ ߦᄌൻߔࠆߣߣ߽ߦ‫ߡߞࠃߦࡦ࠱ࠗࠚࡈޔ‬േ⊛ߦ߽ំࠄ޿ߢ޿ࠆߎߣ߇⍮ࠄࠇߡ޿ࠆ‫ޕ‬㒢ࠄࠇߚ႐ว ࠍ㒰߈‫ߥ߁ࠃߩߎޔ‬᭴ㅧߪࡍࡦࡠ࡯࠭࠲ࠗ࡝ࡦࠣߦࠃߞߡᱜ⏕ߦ⸥ㅀߔࠆߎߣߪ಴᧪ߥ޿‫ޕ‬Ḱ⚿᥏᭴ ㅧߣߘߩ቟ቯᕈࠍ♽⛔⊛ߦℂ⸃ߔࠆߚ߼ߦߪ‫ࠅࠃޔ‬ᄙߊߩ⒳㘃ߩ࠲ࠗ࡝ࡦࠣࠍ⠨ኤߔࠆᔅⷐ߇޽ࠆ‫ޕ‬ એ਄ߩⷰὐ߆ࠄ‫⎇ᧄޔ‬ⓥߢߪ㪈㪇࿁ኻ⒓Ḱ๟ᦼ࠲ࠗ࡝ࡦࠣࠍ↢ᚑߔࠆ৻⥸⊛ᚻᴺࠍ⠨᩺ߒ‫ޔ‬᭽‫ߥޘ‬ᣂߒ ޿࠲ࠗࡊߩ࠲ࠗ࡝ࡦࠣߩ૞ᚑࠍ⹜ߺߚ‫ޕ‬㩷 ᧄᚻᴺߢߪ‫ߣ࡞࡯࡞ࡦ࡚ࠪ࡯࡟ࡈࡦࠗޔ‬๭߫ࠇࠆ⥄Ꮖ⋧ૃᄌ឵ⷙೣߩ➅ࠅ㄰ߒߦࠃߞߡή㒢ߦᄢ߈ ߥḰ๟ᦼ࠲ࠗ࡝ࡦࠣࠍ↢ᚑߔࠆ‫ߪ࡞࡯࡞ࡦ࡚ࠪ࡯࡟ࡈࡦࠗޕ‬㧔㧝㧕⋧ૃᄌ឵㧔᜛ᄢᄌ឵‫ޔ‬᜛ᄢᲧ₸ߪ 㪈㪇࿁ኻ⒓ࡉ࡜ࡌട⟲ߩ㪧㫀㫊㫆㫋න૏㩷 Ǽ㧔㤛㊄Ყ㧕ߩ⥄ὼᢙਸ਼߆ࠄㆬᛯ㧕‫ޔ‬෸߮㧔㧞㧕᜛ᄢߒߚฦ࠲ࠗ࡞ߩ ಽഀ‫ߩޔ‬㪉ࠬ࠹࠶ࡊ߆ࠄߥࠆ‫ࡊ࠶࠹ࠬޕ‬㧔㧞㧕㪄࠲ࠗ࡞ߩಽഀ㪄ߦὐ↢ᚑࡊࡠ࠮ࠬࠍ੺࿷ߐߖࠆߎߣߢ‫ޔ‬ ಽഀᣇᴺࠍኈᤃߦ৻⥸ൻߔࠆߎߣ߇಴᧪ࠆ‫ޔࠅࠃߦࠇߘޕ‬᭽‫ࠆߥ⇣ߥޘ‬⒳㘃ߩ࠲ࠗ࡝ࡦࠣࠍ૞ᚑߔࠆ ߎߣ߇಴᧪ࠆࠃ߁ߦߥߞߚ‫ޕ‬㩷 ਄⸥ߩᚻᴺࠍ↪޿ߡᧄ⎇ⓥߢ⊒⷗ߒߚ࠲ࠗ࡝ࡦࠣߩ଀ߣߒߡ‫ޔ‬㪊⒳㘃ߩࡊࡠ࠻࠲ࠗ࡞㧔㪊㪍ᐲߩ⪉ᒻ‫ޔ‬ ᱜ㩷㪌ⷺᒻ‫ޔ‬ᮻဳ㪍ⷺᒻ㧕ࠍᜬߟᣂߒ޿࠲ࠗࡊߩ㪈㪇࿁ኻ⒓࠲ࠗ࡝ࡦࠣ㩿࿑㪍㩿Ꮐ㪀㪀ࠍขࠅ਄ߍࠆ‫࡝ࠗ࠲ߩߎޕ‬ ࡦࠣߩࠗࡦࡈ࡟࡯࡚ࠪࡦ࡞࡯࡞㧔㪠㪩㪄㪈㧕ߪ㏜ᤋኻ⒓ᕈࠍ⎕ࠆߩߢ‫↢ޔ‬ᚑߒߚ࠲ࠗ࡝ࡦࠣߪࠠ࡜࡝࠹ࠖ ࠍᜬߟ‫ߩࠣࡦ࡝ࠗ࠲ޕ‬㗂ὐࠍ⋥੤⵬ⓨ㑆ߦᛩᓇߔࠆߣ‫ߥ࡞࠲ࠢ࡜ࡈޔ‬Ⴚ⇇ࠍᜬߟ⓹߇ᓧࠄࠇࠆ㧔࿑㪍㧔ฝ㧕㧕‫࡞࡯࡞ࡦ࡚ࠪ࡯࡟ࡈࡦࠗޕ‬㧔㪠㪩㪄㪈㧕ߩ㏜ᤋࠍߣߞߚ߽ߩࠍ㧔㪠㪩㪄㪉㧕ߣ⴫ߔߎߣߦߔࠆ‫ޕ‬㪉⒳㘃 ߩࠗࡦࡈ࡟࡯࡚ࠪࡦ࡞࡯࡞㧔㪠㪩㪄㪈㧕ߣ㧔㪠㪩㪄㪉㧕ࠍછᗧߩ㗅ᐨߢㆡ↪ߔࠆߎߣߢ‫ߦࠄߐޔ‬ή㒢ߦᄙߊߩ⒳ 㘃ߩ࠲ࠗ࡝ࡦࠣࠍ૞ᚑߔࠆߎߣ߇น⢻ߣߥࠆ‫ߩࠄࠇߎޕ‬ή㒢⒳㘃ߩ࠲ࠗ࡝ࡦࠣߪ੕޿ߦࡈࠚࠗ࠱ࡦࡈ ࡝࠶ࡊߦࠃࠅ㑐ଥઃߌࠄࠇߡ޿ࠆ‫ޕ‬Ḱ⚿᥏ߩ᭴ㅧ቟ቯൻߦኻߔࠆࡈࠚࠗ࠱ࡦំࠄ߉ߩലᨐࠍ⼏⺰ߔࠆ ਄ߢ‫ߥ↪᦭ߪࠣࡦ࡝ࠗ࠲ߩࠄࠇߎޔ‬⍮⷗ࠍਈ߃ࠆน⢻ᕈ߇޽ࠆߣ⠨߃ߡ޿ࠆ‫ޕ‬㩷. ࿑ 6 (Ꮐ) 3 ⒳㘃ߩ࠲ࠗ࡞߆ࠄߥࠆ࠲ࠗ࡝ࡦࠣ‫ޔ‬㧔ฝ㧕ࡈ࡜ࠢ࠲࡞ߥႺ⇇ࠍᜬߟභ᦭⓹. 㩷.

(15) 研究活動報告. 䇼⎇ⓥᵴേႎ๔䇽㩷㩷 ᢎ ಎ ഥ. ↢૕ᯏ⢻⸳⸘⎇ⓥಽ㊁䋨2008䋮1䌾㪉㪇㪇㪏䋮12䋩㩷 ᝼㩷㩷㪑ਅ᧛㩷 ᡽༹㩷 ᝼ 㩷㩷㪑↰ਛ㩷⾫㩷 ᢎ㩷㩷㪑⮳㩷 ᶈ㩷. ᢎ. ഥ㩷㩷㩷㩷㩷㩷 ᢎ㩷㩷䋺⍹੗㩷 ᄢ૓䋨㪮㪧㪠䋩㩷 ಎ ⡯ ຬ㩷㩷䋺㩷䊒䊥䉝䉴䊃䉡䉼䉟㩷䉡䊤䊮䉻䊥㩷 ቇᝄ․೎⎇ⓥຬ䋺ᮘญ㩷 ೰ᔒ䋨㪮㪧㪠䋩㩷 ᄢ ቇ 㒮 ↢㩷㩷㪑㩷 ఽፉ㩷 ⟤ቄ䇮ᐔ੗㩷 ᖘม䇮⨹㩷㓷ᶈ䇮ᧄศ㩷 ⓥ㩷 ቇ ㇱ ቇ ↢㩷㩷㪑ዊᳰ㩷 ๺ቁ䇮ખ㆏㩷 ⵨⾆㩷㩷. 㩷. 㩷. 㩷. ᧄ⎇ⓥಽ㊁ߢߪ㧘⥄Ꮖ⚵❱ൻ࠰ࡈ࠻ࡑ࠹࡝ࠕ࡞ߩ૞⵾ߣᯏ⢻ߦߟ޿ߡ⎇ⓥᵴേࠍⴕߞߡ޿ࠆ㧚 ᐕ ߩ⎇ⓥᵴേߣߒߡߪ㧘એਅߩࠃ߁ߦ᭎᜝ߐࠇࠆ㧚㩷㧝.⥄Ꮖ⚵❱ൻߦࠃࠆࡂ࠾ࠞࡓ᭴ㅧࡈࠖ࡞ࡓߩ૞⵾ߣᯏ⢻ߦ㑐ߔࠆ⎇ⓥ㩷㧔㧝㧕ࡂ࠾ࠞࡓ᭴ㅧࡈࠖ࡞ࡓߩ᠘᳓ᕈ೙ᓮߦ㑐ߔࠆ⎇ⓥ ᧄ⎇ⓥቶߢߪ‫⇹ޔ‬᳓ᕈ㜞ಽሶṁᶧࠍ࿕૕ၮ᧼਄ߦႣᏓߒടḨ㔓࿐᳇ਅߢ⵾⤑ߔࠆߎߣߦࠃࠅ‫࠾ࡂޔ‬ ࠞࡓ⁁ᄙሹ⾰⤑߇⥄Ꮖ⚵❱⊛ߦᒻᚑߐࠇࠆߎߣࠍ⷗޿ߛߒߡ޿ࠆ‫᦭ޕ‬ᯏṁᇦ߇ṁᶧ߆ࠄ⫳⊒ߔࠆ㓙ߩ ᳇ൻᾲߦࠃࠅṁᶧ⴫㕙߇಄ළߐࠇ‫ޔ‬ടḨ㔓࿐᳇ਛߩ᳓⫳᳇߇⚿㔺ߒߡṁᶧ⴫㕙ߦᒻᚑߒߚ᳓Ṣ߇Ძ▤ ജߦࠃࠅ⚦ኒలႯߒ‫ޔ‬ṁᇦ߅ࠃ߮᳓Ṣ߇⫳⊒ߒߚᓟߦ㜞ಽሶࡈࠖ࡞ࡓਛߦⓨሹ߇ᒻᚑߐࠇࠆߢ޽ࠆ‫ޕ‬ 㜞ಽሶṁᶧߦዋ㊂ߩਔⷫᇦᕈࡐ࡝ࡑ࡯ࠍᷝടߔࠆߎߣߢ‫⚿ޔ‬㔺ߒߚ᳓Ṣ߇቟ቯൻߐࠇ᳓Ṣ㑆ߢߩⲢว ߇ᅹߍࠄࠇ‫ޔ‬ဋ৻ߥሹᓘࠍ᦭ߔࠆ⚦ሹ߇ⷙೣ㈩೉ߒߚ᭴ㅧ૕㧔ࡂ࠾ࠞࡓ᭴ㅧࡈࠖ࡞ࡓ㧕߇ᒻᚑߐࠇࠆ‫ޕ‬ ߹ߚ‫ࡓࠞ࠾ࡂޔ‬᭴ㅧࡈࠖ࡞ࡓࠍ‛ℂ⊛ߦ೸㔌ߔࠆߎߣߦࠃߞߡ‫ޔ‬ᓸዋߥ⓭⿠߇ⷙೣ⊛ߦ㈩೉ߒߚࡇ࡜ ࡯⁁᭴ㅧ૕ࠍ૞⵾ߔࠆߎߣ߇಴᧪ࠆ‫⴫ߩ⪲ߩ⬒ޕ‬㕙߇⿥᠘᳓ᕈࠍ␜ߔߎߣߢ⍮ࠄࠇߡ޿ࠆࠃ߁ߦ‫⴫ޔ‬ 㕙ߩࡒࠢࡠߥಳಲ᭴ㅧߪ᳓߿ṁᇦߦኻߔࠆỨࠇᕈࠍᒝ⺞ߔࠆ૞↪߇޽ࠅ‫⁁࡯࡜ࡇޔ‬᭴ㅧ૕ߩ⴫㕙ߪ᠘ ᳓⴫㕙ߣߥࠆ‫ޕ‬ 0࠼࠺ࠪ࡞ࠕࠢ࡝࡞ࠕࡒ࠼ߣⷫ᳓ᕈࠕࠢ࡝࡞ࡕࡁࡑ࡯ߣߩ౒㊀ว૕ߪࡂ࠾ࠞࡓ᭴ㅧࡈࠖ࡞ࡓߩ૞⵾ ߦㆡߒߚਔⷫᇦᕈࡐ࡝ࡑ࡯ߢ޽ࠆ‫ޕ‬᷷ᐲᔕ╵ᕈߩࡕࡁࡑ࡯ߢ޽ࠆ 0ࠗ࠰ࡊࡠࡇ࡞ࠕࠢ࡝࡞ࠕࡒ࠼ߣ 0 ࠼࠺ࠪ࡞ࠕࠢ࡝࡞ࠕࡒ࠼ߩ౒㊀ว૕ࠍᣂⷙߦวᚑߒߚ‫ߩߎޕ‬㜞ಽሶߩⷫ᳓ᕈߪ᷷ᐲߦࠃߞߡ೙ᓮߔࠆ ߎߣ߇ߢ߈ࠆ‫ޕ‬᷷ᐲᔕ╵ᕈࠕࠢ࡝࡞ࠕࡒ࠼౒㊀ว૕ߣࡐ࡝ࠬ࠴࡟ࡦߩࠢࡠࡠࡎ࡞ࡓṁᶧ߆ࠄࡂ࠾ࠞࡓ ᭴ㅧࡈࠖ࡞ࡓߣࡇ࡜࡯⁁᭴ㅧ૕ࠍ૞⵾ߒ‫⴫ߩࠄࠇߘޔ‬㕙਄ߦ߅ߌࠆ᳓ߩធ⸅ⷺߩ᷷ᐲଐሽᕈࠍ᷹ቯߒ ߚ‫ࡓࠞ࠾ࡂޕ‬᭴ㅧࡈࠖ࡞ࡓߩធ⸅ⷺߪ޽߹ࠅ᷷ᐲߦଐሽߖߕ‫ ߘࠃ߅߅ޔ‬ᐲ⒟ᐲߢ޽ߞߚ‫৻ޕ‬ᣇ‫ޔ‬ ࡇ࡜࡯⁁᭴ㅧ૕ߢߪ ͠ࠍႺߦ㜞᷷ߢߪ ᐲએ਄ߩ⿥᠘᳓ᕈࠍ␜ߒ‫ޔ‬ ૐ᷷ߢߪ ᐲ⒟ߦᷫዋߒߚ 㧔࿑ 㧝㧕 ‫ޕ‬᳓Ṣߩ቟ቯൻࠍ߽ߚࠄߔਔⷫᇦᕈࡐ࡝ࡑ࡯ߦ᷷ᐲᔕ╵ᕈၮࠍዉ౉ߔࠆߎߣߢ‫ޔ‬᷷ᐲᄌൻߦᔕ╵ߒ ߡ⴫㕙Ứࠇᕈ߇ᄌൻߔࠆ⥄Ꮖ⚵❱ൻ᧚ᢱߩ૞⵾ߦᚑഞߒߚ‫ޕ‬ ᰴߦ‫ޔ‬ᨋ┙ߔࠆࡇ࡜࡯⁁⓭⿠ߩਛߦⷫ᳓ᕈߩ㊄ዻ࠼ࡔࠗࡦࠍⶄวൻߔࠆߎߣߢ‫ޔ‬᳓Ṣๆ⌕⢻ജࠍ᦭ ߔࠆ⿥᠘᳓⴫㕙ࠍ૞⵾ߒߚ‫⴫ߩߎޕ‬㕙ߪ‫ޔ‬᳓Ṣធ⸅ⷺ߇ ᐲએ਄ߩ⿥᠘᳓⴫㕙ߢ޽ࠅߥ߇ࠄ‫ޔ‬ၮ᧼. 12.

(16) 13. 研究活動報告. ࠍ෻ォߐߖߡ߽᳓Ṣࠍ଻ᜬߔࠆߎߣ߇ߢ߈ߚ‫࡯࡜ࡇࡦ࡟࠴ࠬ࡝ࡐޕ‬᭴ㅧਛߦಽᏓߒߚ࠾࠶ࠤ࡞࠼࡯ࡓ ߩഀวࠍჇടߐߖࠆߣ‫ޔ‬ធ⸅ⷺߪૐਅߔࠆ߽ߩߩ⿥᠘᳓⴫㕙ࠍ⛽ᜬߒߟߟ‫ޔ‬᳓Ṣߩォ⪭ⷺᐲߪᄢ߈ߊ ߥࠆ‫ޕ‬࿑㧞ߦ␜ߔࠃ߁ߦ‫ޔ‬㊄ዻ࠼࡯ࡓߩዉ౉߇ 㧑ࠍ⿥߃ࠆߣၮ᧼ࠍ෻ォߒߡ߽ OI ߩ᳓Ṣࠍ଻ᜬߔ ࠆๆ⌕ജ߇ᓧࠄࠇߚ‫ޕ‬. . . Fig.1. Thermal wettability control of the pin-cushion structured surface prepared from the honeycomb-patterned polymer film.. Fig.2. Contact angles 䋨䃂䋩 and sliding angles 䋨䂾䋩 of 5.0mg-water droplet on the hybrid nickel-dome㺍polystyrene-spike films. The inset shows a photograph of the water droplet on an inverted film with a dome density of 25%. 㧔㧞㧕ࡂ࠾ࠞࡓ᭴ㅧࡈࠖ࡞ࡓࠍၮ᧚ߣߔࠆ⚦⢩ၭ㙃ߦ㑐ߔࠆ⎇ⓥ ߔߢߦᚒ‫ࡓࠞ࠾ࡂޔߪޘ‬᭴ㅧࡈࠖ࡞ࡓ߇᦭ߔࠆ⴫㕙ᓸ⚦᭴ㅧ߇⚦⢩ߩჇᱺ‫ޔ‬ಽൻ‫ޔ‬ᒻᘒߥߤߦ⪺ߒ ޿ᓇ㗀ࠍ߽ߚࠄߔߎߣࠍ⷗޿ߛߒߡ޿ࠆ‫੹ޕ‬ᐕᐲߪ‫ޔ‬᭽‫ߥޘ‬ሹᓘࠍ᦭ߔࠆࡂ࠾ࠞࡓ᭴ㅧࡈࠖ࡞ࡓ਄ߢ 㛽⧘⚦⢩ࠍၭ㙃ߒ‫⴫⤑ޔ‬㕙ߩᓸ⚦ᒻ⁁߇㛽⧘⚦⢩ߩធ⌕᜼േ‫ޔ‬Ⴧᱺ‫ޔ‬㛽ᒻᚑᯏ⢻ߦ෸߷ߔᓇ㗀ߦߟ޿.

(17) 研究活動報告. 14. ߡ⺞ߴߚ‫ࠄ߆࠻࠶࡜ޕ‬㗡⬄㛽ࠍಾ㒰࿁෼ߒ‫ޔ‬㛽⧘⚦⢩ࠍಽ㔌ߒߚ‫ޕ‬ᚲቯᤨ㑆ၭ㙃ᓟ‫⚦ޔ‬⢩ធ⌕ᢙ‫⚦ޔ‬ ⢩ᒻᘒ‫⚦ޔ‬⢩㛽ᩰ㧔ࠕࠢ࠴ࡦ㧕‫ޔ‬ធ⌕⃰㧔ࡆࡦࠠࡘ࡝ࡦ㧕‫ࡓ࠙ࠪ࡞ࠞ߮ࠃ߅࠯࡯࠲ࠔࡈࠬࡎ࡝ࠞ࡞ࠕޔ‬ ࠍᨴ⦡ᓟ‫↹ޔ‬௝⸃ᨆࠍⴕ޿‫ޔ‬㛽ᒻᚑ㊂ࠍቯ㊂ൻߒߚ‫⿛ޔߚ߹ޕ‬ᩏဳ㔚ሶ㗼ᓸ㏜㧔5'/㧕‫࡯ࠡ࡞ࡀࠛޔ‬ಽ ᢔဳ : ✢ಽᨆⵝ⟎㧔'&#:㧕ߦࠃࠅ㛽ᒻᚑࠍⷰኤߒߚ‫ ߦࠄߐޕ‬462%4 ߦࠃࠅ㛽⚦⢩ߩಽൻࡑ࡯ࠞ࡯ߩ⸃ ᨆࠍⴕߞߚ‫ޕ‬ ၭ㙃 ᤨ㑆ᓟ߹ߢߪ‫ޔ‬ฦၮ᧼ߩធ⌕⚦⢩ᢙߪ߶߷ห᭽ߢ޽ߞߚ‫ޕ‬ᐔ⤑਄ߩ⚦⢩ߪ‫ࡓࠞ࠾ࡂޔ‬᭴ㅧࡈ ࠖ࡞ࡓ਄ߩ⚦⢩ߦᲧߴ╬ᣇ⊛ߦિዷߒធ⌕㕙Ⓧ߇⚂ ୚ᄢ߈߆ߞߚ‫ޔߦ․ޕ‬ሹᓘ ǴO ߩ ࡂ࠾ࠞࡓ᭴ㅧࡈࠖ࡞ࡓ਄ߢߪ‫⚦ޔ‬⢩ߪ⚦㐳ߊિዷߒ‫⚦ޔ‬⢩ߩ๟࿐㐳߇㐳޿ߎߣ߇ࠊ߆ߞߚ‫ࡘࠠࡦࡆޕ‬ ࡝ࡦᨴ⦡ߦࠃࠆធ⌕⃰㧔ǴO㧕ߩ⸃ᨆࠍⴕߞߚߣߎࠈ‫ޔ‬ᐔ⤑਄ߩ⚦⢩ࠃࠅሹᓘ ǴO ߩ ࡂ࠾ࠞࡓ᭴ㅧࡈࠖ࡞ࡓ਄ߩ⚦⢩ߩᣇ߇㜞ኒᐲߩធ⌕⃰߇⊒⃻ߒߡ޿ࠆߎߣ߇ࠊ߆ߞߚ‫ޔࠄ߆ߣߎߩߎޕ‬ ធ⌕⃰ࠍ੺ߒߚჇᱺ߿ᯏ⢻⊒⃻ߥߤߩࠪࠣ࠽࡞વ㆐߇↢ߓߡ޿ࠆߎߣ߇␜ໂߐࠇߚ‫ޕ‬ၭ㙃 ‫ᤨ ޔ‬㑆 ᓟߪ‫ޔ‬ሹᓘ ǴO ߩࡂ࠾ࠞࡓ᭴ㅧࡈࠖ࡞ࡓ਄ߩ⚦⢩ᢙߪჇടߔࠆߩߦኻߒߡ‫ޔ‬ሹᓘ Ǵ O ߩࡂ࠾ࠞࡓ⤑਄ߢߪ⚦⢩ᢙߩᷫዋ߇᷹ⷰߐࠇߚ‫ޕ‬ၭ㙃 ㅳ㑆ᓟߪ‫ޔ‬ሹᓘ ǴO ߩࡂ࠾ࠞࡓ᭴ ㅧࡈࠖ࡞ࡓ਄ߩ⚦⢩ߪᐔ⤑਄ߩ⚦⢩ߦᲧߴࠝࠬ࠹ࠝࠞ࡞ࠪࡦߥߤߩ㛽ಽൻࡑ࡯ࠞ࡯ߩ਄᣹߇⷗ࠄࠇߚ‫ޕ‬ ࿑㧟ߦၭ㙃 ᣣᓟߩࠕ࡞ࠞ࡝ࡎࠬࡈࠔ࠲࡯࠯߅ࠃ߮ࠞ࡞ࠪ࠙ࡓߩᨴ⦡௝‫ޔ‬㛽ᒻᚑ㊂ߩቯ㊂⚿ᨐࠍ␜ߔ‫ޕ‬ ฦၮ᧼਄ߢ㛽⧘⚦⢩߇ⷒߞߡ޿ࠆߎߣ߇⏕⹺ߐࠇߚ‫ޕ‬ሹᓘ ǴO ߩࡂ࠾ࠞࡓ᭴ㅧࡈࠖ࡞ࡓ਄ ߩ⚦⢩ߪᐔ⤑਄ߩ⚦⢩ߦᲧߴ㜞޿㛽ᒻᚑ⢻߇⹺߼ࠄࠇߚ‫ޕ‬ . Fig.3. Enhanced matrix mineralisation by osteoblasts on honeycomb-patterned films. Staining at the Day 28 showed extensive areas of alkaline phosphatase positive (blue) cells surrounding Alizarin red stained mineralised matrix. Quantification of bone formation reveals that 3-4ȝm and 5-6ȝm honeycomb films support greater production of matrix compared to flat film. .

(18) 15. 研究活動報告. 5'/ ߅ࠃ߮ '&#: ⷰኤߩ⚿ᨐ‫ࡓࠞ࠾ࡂޔ‬᭴ㅧࡈࠖ࡞ࡓߩࡈ࡟࡯ࡓࡢ࡯ࠢߩ⴫㕙ߥࠄ߮ߦሹߩౝㇱߦ࡝ ࡦ㉄ࠞ࡞ࠪ࠙ࡓߩ⚿᥏ߩᨆ಴߇⹺߼ࠄࠇߚ‫ޕ‬એ਄ߩ⚿ᨐ߆ࠄ‫ޔ‬ሹᓘ ǴO ߩࡂ࠾ࠞࡓ⤑ߪ㛽 ⧘⚦⢩ߩಽൻߣ㛽ᒻᚑ⢻ࠍะ਄ߐߖࠆߎߣ߇ࠊ߆ߞߚ‫ࡓࠞ࠾ࡂޔߚ߹ޕ‬᭴ㅧࡈࠖ࡞ࡓߦࡂࠗ࠼ࡠࠠࠪ ࠕࡄ࠲ࠗ࠻ࠍᨆ಴ߐߖߚ⴫㕙ߢߪ‫ޔ‬㛽⧘⚦⢩ᯏ⢻ߩߐࠄߥࠆะ਄߇ᦼᓙߢ߈ࠆ‫ࡓࠞ࠾ࡂޕ‬᭴ㅧࡈࠖ࡞ ࡓߦ߅ߌࠆᮮᣇะߩ⽾ㅢሹห჻ߩㅪㅢ᭴ㅧߪ‫ࡓࠞ࠾ࡂޔ‬᭴ㅧࡈࠖ࡞ࡓ਄ߢჇᱺߔࠆ㛽⧘⚦⢩㑆ߩ⋧੕ ૞↪߇น⢻ߢ޽ࠅ‫ޔߚ߹ޔ‬ၭ࿾ਛߩᩕ㙃ಽ߿ṁሽ㉄⚛ߩଏ⛎‫ޔ‬⠧ᑄ‛ߩឃ಴߇ࠬࡓ࡯࠭ߦⴕࠊࠇࠆߥ ߤߩ೑ὐࠍ߽ߚࠄߔߣ⠨߃ࠄࠇ‫ޔ‬㑐▵ᴦ≮‫ޔ‬㛽࡮エ㛽∔ᖚߦኻߔࠆ⎬⚵❱ౣ↢ᴦ≮߳ߩዷ㐿߇ᦼᓙߢ ߈ࠆ‫⎇ᧄޕ‬ⓥߪ‫⧷ޔ‬࿖࠾ࡘ࡯ࠞ࠶ࠬ࡞ᄢቇකቇㇱ /CTM$KTEJ ඳ჻ߥࠄ߮ߦർᶏ㆏ᄢቇഃᚑ⑼ቇ⎇ⓥᯏ ᭴ጊᧄ⽵᣿ᢎ᝼ߣߩ౒ห⎇ⓥߢ޽ࠆ‫ޕ‬ ᰴߦ‫ࡓࠞ࠾ࡂޔ‬᭴ㅧࡈࠖ࡞ࡓࠍ↪޿ߡⴊ▤⚵❱ౣ↢ࠍᬌ⸛ߔࠆߚ߼ߦ‫ߩ▤ⴊޔ‬᭴ᚑ⚦⢩ߢ޽ࠆᐔṖ ╭⚦⢩ߩၭ㙃ࠍⴕߞߚ‫ޕ‬ᚲቯᤨ㑆ၭ㙃ᓟ‫⚦ޔ‬⢩ࠍ᛫૕Ⱟశᨴ⦡ߒ‫౒ޔ‬ὶὐ࡟࡯ࠩ࡯⿛ᩏဳ㗼ᓸ㏜ %.5/ ߢⷰኤࠍⴕ޿‫⚦ޔ‬⢩ኒᐲ㨯ធ⌕ᒻᘒࠍ⹏ଔߒߚ‫ࡓࠞ࠾ࡂޕ‬᭴ㅧࡈࠖ࡞ࡓ‫ޔ‬ᐔ⤑਄ߢߪ⚦⢩ኒᐲ߇⇣ߥࠆ ߎߣ߆ࠄ‫ޔ‬ೋᦼធ⌕₸㨯⚦⢩ჇᱺߦᏅ⇣߇޽ࠆߎߣ߇ࠊ߆ߞߚ‫⚦ޔߪࠇߎޕ‬⢩߇⿷႐ߩ࠻ࡐࡠࠫ࡯ߩ㆑ ޿ࠍ⹺⼂ߒធ⌕ߒߚߎߣߦ↱᧪ߔࠆߣ⠨߃ࠄࠇࠆ‫ޕ‬%.5/ ↹௝߆ࠄ‫⚦ޔ‬⢩ߪᐔ⤑਄ߢߪᐢߊ⭯ߊિዷߒ 㧔࿑㧠C㧕‫৻ޔ‬ᣇ‫ࡓࠞ࠾ࡂޔ‬᭴ㅧࡈࠖ࡞ࡓ਄ߢߪ⚦㐳޿ᒻᘒߢ㈩ะߒߡធ⌕ߔࠆ㧔࿑㧠㨎㧕௑ะ߇ⷰኤ ߐࠇߚ‫ޔߪࠇߎޕ‬ᐔ⤑਄ߢߪ⚦⢩ធ⌕ߩ⿷႐ߦ೙㒢߇ߥ޿ߚ߼ᄢ߈ߊિዷߢ߈ࠆ߇‫ࡓࠞ࠾ࡂޔ‬᭴ㅧࡈ ࠖ࡞ࡓ਄ߢߪⓨሹㇱಽߦࠃࠅ⚦⢩ធ⌕߇೙㒢ߐࠇߚߚ߼ߣ⠨߃ࠄࠇࠆ‫ࡓࠞ࠾ࡂޕ‬᭴ㅧࡈࠖ࡞ࡓߩሹᓘ ߇ዊߐ޿߶ߤ‫⚦ޔ‬⢩ߪࠃߊિዷߒ㨯Ⴧᱺᕈ߇㜞޿௑ะࠍ␜ߒߚ‫ޕ‬ᐔ⤑਄ߣᲧセߒ‫ࡓࠞ࠾ࡂޔ‬᭴ㅧࡈࠖ࡞ ࡓ਄ߩᐔṖ╭⚦⢩ߪෘߺ߇޽ࠅ‫ߩߢ❱⚵▤ⴊޔߚ߹ޔ‬ធ⌕ᒻᘒߣ㘃ૃߒߡ޿ࠆߚ߼‫⚦ޔ‬⢩ᯏ⢻ߩะ਄ ߇ᦼᓙߢ߈ࠆ‫ޕ‬ . Fig.4. CLSM images of cells cultured on Flat and Honeycomb-patterned (HC) films. The cytoskelton protein actin filaments and vinculin are stained using imunofluoresence..