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(1)東北大学 多元物質科学研究所. 研 究 業 績・活 動 報 告 2016 年 (平成 28 年)12 月.

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(3) 多元物質科学研究所. 研究業績・活動報告 目 次 研究活動報告 ·············································································· 有機・生命科学研究部門 ········································································································. 1 1. 無機材料研究部門 ················································································································· 14 プロセスシステム工学研究部門 ······························································································· 27 計測研究部門 ······················································································································· 45 サステナブル理工学研究センター ···························································································· 67 先端計測開発センター ··········································································································· 85 高分子・ハイブリッド材料研究センター ··················································································· 93 新機能無機物質探索研究センター ···························································································· 107 その他 ································································································································ 118. 研究会報告 ·················································································123 学会発表講演目録 ········································································156 有機・生命科学研究部門 ········································································································ 156 無機材料研究部門 ················································································································· 158 プロセスシステム工学研究部門 ······························································································· 160 計測研究部門 ······················································································································· 163 サステナブル理工学研究センター ···························································································· 166 先端計測開発センター ··········································································································· 169 高分子・ハイブリッド材料研究センター ··················································································· 171 新機能無機物質探索研究センター ···························································································· 173 その他 ································································································································ 175. 研究業績目録 ··············································································176 有機・生命科学研究部門 ········································································································ 176 無機材料研究部門 ················································································································· 178 プロセスシステム工学研究部門 ······························································································· 183 計測研究部門 ······················································································································· 187 サステナブル理工学研究センター ···························································································· 191 先端計測開発センター ··········································································································· 196 高分子・ハイブリッド材料研究センター ··················································································· 199 新機能無機物質探索研究センター ···························································································· 203 その他 ································································································································ 206. 業績目録著者索引 ········································································208.

(4) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌. 研究活動報告. 1. ⏕࿨ᶵ⬟ศᏊྜᡂ໬Ꮫ◊✲ศ㔝. ࠙◊✲άືሗ࿌ࠚ. 䠄2016. 1䡚2016. 12䠅. ᩍ 㻌 㻌 ᤵ䠖Ọḟ ྐ ຓ 㻌 㻌 ᩍ䠖㨣ሯ ࿴ග, ᒣ⏣ ◊ ஦ົ⿵బဨ䠖ᮌᮧ ⌮㤶 ኱ Ꮫ 㝔 ⏕䠖బ⸨ ᠇኱(䡚2016ᖺ3᭶), ⛅⃝ ᣅஓ(䡚2016ᖺ3᭶), బ䚻ᮌ Ḡᏹ (䡚2016ᖺ3᭶), 㞵ᐑ ᣅဢ (䡚2016ᖺ3᭶), 㜿㒊 ཭ு (䡚2016ᖺ3᭶), ᑠᯘ ೔ோ (䡚2016ᖺ3᭶), Ᏹబ⨾ ᙯ, ᑠᖹ ೺ኴ, ᐑୗ ༟ஓ, Hazemi Madoka Eurika, ୹㔝 ᏹு (2016ᖺ4᭶䡚), ⏣ཱྀ ᫭ྐ(2016ᖺ4᭶䡚) ᮏ◊✲ศ㔝䛷䛿䚸᭷ᶵ໬Ꮫ䜢䝧䞊䝇䛻ᶵ⬟ᛶศᏊ䜢タィ䞉ྜᡂ䛧䚸䛭䜜䜙䜢⏝䛔䛶㑇ఏᏊⓎ⌧䜢໬Ꮫⓗ䛻 ไᚚ䛩䜛᪉ἲㄽ䛾㛤Ⓨ䜢┠ᣦ䛧䛶◊✲άື䜢⾜䛳䛶䛔䜛䚹2016ᖺ䛾ᮏ◊✲ᡤ䛻䛚䛡䜛୺䛺◊✲άື䛸䛧䛶䛿䚸 ௨ୗ䛾䜘䛖䛻ᴫᣓ䛥䜜䜛䚹.  ᰾㓟㧗ḟᵓ㐀䛻䛚䛡䜛㑅ᢥⓗ䜰䝹䜻䝹໬཯ᛂ䛾㛤Ⓨ ㏆ᖺ䚸᰾㓟䛜ᙧᡂ䛩䜛㧗ḟᵓ㐀䛿㑇ఏᏊⓎ⌧ไᚚᶵᵓ䛻䛚䛔䛶㔜せ䛺ᙺ๭䜢ᣢ䛴䛣䛸䛜ศ䛛䛳䛶䛝䛶䛚 䜚䚸䛣䜜䜙䛾ᵓ㐀䛻⤖ྜ䛩䜛䝥䝻䞊䝤䛾㛤Ⓨ䛜᳨ウ䛥䜜䛶䛔䜛䚹ᡃ䚻䛾◊✲ᐊ䛷䛿䛩䛷䛻䚸᰾㓟㧗ḟᵓ㐀䛾 䝰䝕䝹䛸䛧䛶䚸⬺ሷᇶ㒊఩䜢ᣢ䛴䠎ᮏ㙐DNAෆ䛾䝏䝭䞁䛻ᑐ䛧䛶䚸㠀ᖖ䛻㑅ᢥⓗ䛻཯ᛂ䛩䜛䝥䝻䞊䝤䜢㛤Ⓨ䛧 䛶䛔䜛䚹ᮏᖺᗘ䛿䛣䛾ᵓ㐀䛻ᇶ䛵䛝䚸U-U䝭䝇䝬䝑䝏ᵓ㐀䜢ᣢ䛴2ᮏ㙐RNAᵓ㐀䚸ཬ䜃䜾䜰䝙䞁㇏ᐩ䛺㓄ิ䛷 ᙧᡂ䛥䜜䜛4ᮏ㙐ᵓ㐀䛻ᑐ䛧㑅ᢥⓗ䛻䜰䝹䜻䝹໬䛩䜛ศᏊ䛻䛴䛔䛶᳨ウ䜢⾜䛳䛯䚹U-U䝭䝇䝬䝑䝏ᵓ㐀䛿CUG 䝖䝸䝥䝺䝑䝖䝸䝢䞊䝖䛻䛚䛔䛶䜏䜙䜜䜛ᵓ㐀䛷䛒䜚䚸➽䝆䝇䝖䝻䝣䜱䞊Ⓨ⑕䛾ཎᅉ䛸䜒ゝ䜟䜜䛶䛚䜚䚸䛣䛾ᵓ㐀䜢䜰 䝹䜻䝹໬䛩䜛ศᏊ䛿䛣䜜䜙䛾⑌ᝈ἞⒪ἲ䛻⧅䛜䜛ྍ⬟ᛶ䛜䛒䜛䚹䛥䜙䛻䚸4ᮏ㙐ᵓ㐀䛿ᰁⰍయᮎ➃䛻ぢ䜙䜜䜛 䠍ᮏ㙐䝔䝻䝯䜰㡿ᇦ䛷ᙧᡂ䛥䜜䜛䛣䛸䛜䜟䛛䛳䛶䛝䛶䛚䜚䚸䛣䛾ᵓ㐀䜢䜰䝹䜻䝹໬䛩䜛ศᏊ䛿䚸⒴⣽⬊䛷άᛶ໬ 䛥䜜䛶䛔䜛䝔䝻䝯䝷䞊䝊䛾㜼ᐖ䛻⧅䛜䜛䛸ᮇᚅ䛥䜜䜛䚹. 䐟 U-U䝭䝇䝬䝑䝏ᵓ㐀䛻⤖ྜ䛧䚸䜰䝹䜻䝹໬䛩䜛䝥䝻䞊䝤䛾㛤Ⓨ䠖ᡃ䚻䛿U-U䝭䝇䝬䝑䝏ᵓ㐀䜢䜰䝹䜻䝹໬ 䛩䜛䝥䝻䞊䝤䛸䛧䛶ᅗ䠍䛻♧䛩䝖䝸䜰䝆䞁ㄏᑟయ䜢タィ䛧䛯䚹ᮏศᏊ䛿U-U䝭䝇䝬䝑䝏ᵓ㐀䛻ᑐ䛧䛶Ỉ⣲⤖ྜ 䜢ᙧᡂ䛧཯ᛂⅬ䛜㏆᥋䛩䜛䛣䛸䛷䜰䝹䜻䝹໬䛜㐍⾜䛩䜛䛸ᮇᚅ䛧䛯䚹 ᅗ㸯 88࣑ࢫ࣐ࢵࢳᵓ㐀ࢆ࢔ࣝ࢟ࣝ໬ࡍࡿࣉࣟ ࣮ࣈࡢタィ. ᅗ ࢔ࣝ࢟ࣝ໬ࣉ࣮ࣟࣈࡢ཯ᛂᛶホ౯ 5v Ale. . U U G C GC G C C G CG C G U U. 3v Al.  RNA (Z=U)㻌. 100. Yield (%). Yield (%). 40. U-U ssU U-A. 20 0. 20. 40. 60. 80. 100 120. T-T. . 80. 60. 0. DNA (Z=T)㻌. 100. . 80. R=. CZG GCX GCG C 6FAM5v㻌 GAC CGY CGG C FAM5v㻌. 60 40. C-C. 20 0. Reaction time (h). 0. 20. 40. 60. 80. 100. 120. A-A, G-G U-A. Reaction time (h). 䛣䛾ศᏊ䜢ྜᡂ䛧䚸䛭䛾཯ᛂᛶ䜢ホ౯䛧䛯䛸䛣䜝䚸RNA୰䛾U-U䝭䝇䝬䝑䝏ᵓ㐀䛻ᑐ䛧䛶䛿䜋䛸䜣䛹཯ᛂ䛫䛪 DNA୰䛾T-T䝭䝇䝬䝑䝏ᵓ㐀䛻ᑐ䛧䛶㠀ᖖ䛻㑅ᢥⓗ䛻཯ᛂ䛩䜛䛣䛸䛜䜟䛛䛳䛯䚹.

(5) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌. 研究活動報告. 2. 4ᮏ㙐DNAᵓ㐀䜢䜰䝹䜻䝹໬䛩䜛ศᏊ䛾㛤Ⓨ䠖ᡃ䚻䛿ᅗ3䛻♧䛧䛯పศᏊ䜢⏝䛔䛶䚸䝠䝖䝔䝻䝯䜰㓄ิ䜢ᣢ䛴 4ᮏ㙐DNAᵓ㐀䛻ᑐ䛩䜛䜰䝹䜻䝹໬཯ᛂ䛻䛴䛔䛶᳨ウ䛧䛯䚹䛭䛾⤖ᯝ䚸௒ᅇྜᡂ䛧䛯䝥䝻䞊䝤䛿4ᮏ㙐䛻ᑐ 䛧䛶㑅ᢥⓗ䛻཯ᛂ䛧䚸1ᮏ㙐䛚䜘䜃2ᮏ㙐DNA䛻ᑐ䛩䜛཯ᛂᛶ䛿ప䛔䛣䛸䜢᫂䜙䛛䛻䛧䛯䚹䛥䜙䛻䜰䝹䜻䝹໬䛧 䛯4ᮏ㙐DNA䜢༢㞳䛧䚸䛭䛾CD䝇䝨䜽䝖䝹䜢☜ㄆ䛧䛯䛸䛣䜝䚸ᮍಟ㣭䛾4ᮏ㙐DNA䛸䜋䜌ྠ䛨䝇䝨䜽䝖䝹䜢♧䛧 䛯䚹䛣䛾⤖ᯝ䛿䜰䝹䜻䝹໬䛧䛯4ᮏ㙐ᵓ㐀䛜ᮍಟ㣭䛾4ᮏ㙐DNA䛸㢮ఝ䛾ᵓ㐀䛷䛒䜛䛣䛸䜢♧၀䛧䛶䛔䜛䚹䜎䛯 䜰䝹䜻䝹໬య䛿ᮍಟ㣭䛾4ᮏ㙐DNA䛻ẚ䜉⼥ゎ ᗘ䛜ୖ᪼䛧䛶䛚䜚䚸䜰䝹䜻䝹໬䛻䜘䛳䛶4ᮏ㙐ᵓ㐀䛜Ᏻᐃ ໬䛥䜜䜛䛣䛸䜒♧၀䛥䜜䛯䚹 ᅗ㸱 㸲ᮏ㙐'1$ࡢᵓ㐀࡜࢔ࣝ࢟ࣝ໬ศᏊࡢᵓ㐀. ᅗ㸲 㸲ᮏ㙐'1$࡟ᑐࡍࡿ཯ᛂᛶ. Alkylation yield (%). 40. 䠮䠙. G4. 30. 20. ss-DNA. 10. ds-DNA. 0 0. 20. 40 60 Reaction time (h). 80. 䠎䠊 ᰾㓟⤖ྜ䝍䞁䝟䜽㉁䛻ᑐ䛩䜛ᯫᶫ཯ᛂศᏊ䛾㛤Ⓨ㻌 ᰾㓟⤖ྜ䝍䞁䝟䜽㉁䛿䚸⏕యෆ䛻䛚䛔䛶䚸ᵝ䚻䛺㔜せ䛺ാ䛝䜢♧䛩䛣䛸䛜ศ䛛䛳䛶䛚䜚䚸䛣䛾ᶵ⬟䜢㑅ᢥⓗ 䛻ไᚚ䛩䜛䛣䛸䛿㠀ᖖ䛻ព⩏䛜䛒䜛䛸⪃䛘䜙䜜䜛䚹ᡃ䚻䛿䛩䛷䛻ᶆⓗ᰾㓟䛻ᑐ䛧䛶㓄ิ㑅ᢥⓗ䛛䛴ሷᇶ㑅ᢥ ⓗ䛻ඹ᭷⤖ྜ䜢ᙧᡂ䛩䜛ᯫᶫ཯ᛂᛶ᰾㓟䜢㛤Ⓨ䛧䛶䛔䜛䚹䛣䛾ᯫᶫ཯ᛂᛶ᰾㓟ሷᇶ䛿ᶆⓗ䛻㏆᥋䛩䜛䛣䛸 䛷㧗䛔཯ᛂᛶ䜢♧䛩䛣䛸䜢ሗ࿌䛧䛶䛔䜛䚹௒ᅇ䛣䛾ᵓ㐀䛻ᇶ䛵䛝᪂䛯䛻᰾㓟⤖ྜ䝍䞁䝟䜽㉁䛻ᯫᶫᙧᡂ䛩䜛 ཯ᛂ᰾㓟䜢タィ䛧䛯䚹䛣䛾཯ᛂᛶሷᇶ䛿ᶆⓗ䜾䜰䝙䞁䛻ᑐ䛧䛶Ỉ⣲⤖ྜ䜢ᙧᡂ䛩䜛䛣䛸䛷䚸཯ᛂⅬ䛷䛒䜛䝡䝙 䝹ᇶ䛜2ᮏ㙐䛾䝯䝆䝱䞊䜾䝹䞊䝤 䛻㓄ྥ䛩䜛䛣䛸䛷䚸 ㏆᥋䛧䛯᰾㓟⤖ྜ䝍䞁䝟䜽㉁䛻཯ᛂ䛩䜛䛣䛸䜢ᮇᚅ䛧 䛯䚹ᮏᖺᗘ䛿䜰䝃䜲䜽䝸䝑䜽䛺⢾㒊䜢ᣢ䛴ㄏᑟయ䜢⤌䜏㎸䜣䛰䜸䝸䝂䝚䜽䝺䜸䝏䝗䜢ྜᡂ䛧䚸⺮ⓑ㉁䜈䛾཯ᛂ䛻 䛴䛔䛶᳨ウ䜢⾜䛳䛯䚹௒ᅇ䚸䝰䝕䝹⺮ⓑ㉁䛸䛧䛶䚸䝅䝖䝅䞁䜢䝯䝏䝹໬䛩䜛䝯䝏䝹䝖䝷䞁䝇䝣䜵䝷䞊䝊䛻ᑐ䛩䜛཯ ᛂᛶ䛻䛴䛔䛶᳨ウ䛧䛯䚹䝅䝖䝅䞁䝯䝏䝹䝖䝷䞁䝇䝣䜵䝷䞊䝊䛿䜶䝢䝆䜵䝛䝔䜱䝑䜽䝇䛻㛵䜟䜛㠀ᖖ䛻㔜せ䛺㓝⣲䛷 䛒䜛䚹AOVT⤌䜏㎸䜣䛰䜸䝸䝂䝚䜽䝺䜸䝏䝗䜢ྜᡂ䛧䚸2ᮏ㙐DNA䜢ᙧᡂ䛥䛫䛯ᚋ䚸㓝⣲䛸཯ᛂ䛥䛫䛯䛸䛣䜝䚸ᅗ 䠒䛻♧䛩䜘䛖䛻䚸䝯䝹䜹䝥䝖䜶䝍䝜䞊䝹Ꮡᅾୗ䛻䛚䛔䛶䜒䚸䜰䝹䜻䝹໬཯ᛂ䛜㐍⾜䛩䜛䛣䛸䛜䜟䛛䛳䛯䚹 㻔ᅗ㻡㻕᰾㓟⤖ྜ⺮ⓑ㉁䛻཯ᛂ䛩䜛ᯫᶫศᏊ䛾タィ㻌. 㻔ᅗ㻢㻕䝅䝖䝅䞁䝯䝏䝹䝖䝷䞁䝇䝣䜵䝷䞊䝊䛻ᑐ䛩䜛䜰䝹䜻䝹໬཯ᛂ㻌 5’ 3’. GGGAATTAAAG X GCAAGACCCG3’ CCCTTAATTTC G*CGTTCTGGGC5’ con 1h. 3h. X=. 6h 18h 24h. Nu BSA adduct. DNMT-1. *C= Methylcytosine(MeC). Hydrogen bonding 4-Amino-2-Oxo-6-Vin ylTriazine (AOVT). 3 uM Duplex, 1 uM HhaI DNMT-1, 60 uM S-AdenosylMethionine (SAM), 50 mM Tris-HCl (pH 7.5 ), 㻌 18.5 mM NaCl, 5 mM β-mercaptoethanol, 10 mM EDTA, 24.6 μg/ml BSA 6.2 % Glycerol.㻌 The gel was stained with Framingo fluorescent gel stein.

(6) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌ 研究活動報告. 3. ࠙◊✲άືሗ࿌ࠚ㻌 㻌 ⏕࿨ᶵ⬟ไᚚ≀㉁໬Ꮫ◊✲ศ㔝䠄2016䠊1䡚2016䠊12䠅㻌 ᩍ ᤵ : ࿴⏣ ೺ᙪ ෸ ᩍ ᤵ : Ⲩᮌ ಖᖾ ຓ ᩍ㸸 ᆏᮏ Ύᚿ ᢏ ⾡ ⫋ ဨ : ᾏཎ ኱㍜ ኱ Ꮫ 㝔 ⏕ : ୖᯇ ுᖹ, ዟᮌ ᬸ, Ⳣ஭ ⚈ຍ, ✄ᇉ 㞞ோ, 㯮἟ ៅ, బ⸨ ᓅோ, ㇏ᒸ ኊኴ, す㈅ ຬ㍤, ᯇᓥ ⴌ㤶, Wijak YOSPANYA, ⳥ụ 㝧ኴ, ⚟⯅ ᝆ㔛, ⸨ᇛ ♸ஓ㻌 ᮏ◊✲ศ㔝䛷䛿᰾㓟䜔䝍䞁䝟䜽㉁䛺䛹⏕య㧗ศᏊ䛾䛜䜣⣽⬊≉␗ⓗ᰾㓟་⸆䜈䛾ᒎ㛤䜔⣽⬊ෆྍど໬ ヨ⸆䜈䛾ᒎ㛤䛺䛹ḟୡ௦䜲䞁䝔䝸䝆䜵䞁䝖ᆺ䝘䝜䝞䜲䜸ᶵ⬟ᮦᩱ䜈䛾ᛂ⏝䚸䛥䜙䛻⏕యศᏊ䛾ᵓ㐀ኚ໬䛸ᶵ⬟ ไᚚゎ᫂䜢┠ᣦ䛧䛯㧗᫬㛫ศゎ⬟෇஧Ⰽ䝇䝨䜽䝖䝹 ᐃ⿦⨨䛾㛤Ⓨ䛺䛹⏕࿨໬Ꮫ䛛䜙≀⌮໬Ꮫ䜎䛷ศ㔝ᶓ ᩿ⓗ䛻ᇶ♏䛛䜙ᛂ⏝䜎䛷ᖜᗈ䛔◊✲άື䜢ᒎ㛤䛧䛶䛔䜛䚹2016 ᖺ䛾◊✲άື䛿䚸௨ୗ䛾䜘䛖䛻ᴫᣓ䛥䜜䜛䚹 䠍䠊MMP-9 άᛶ䜢ά⏝䛧䛯᪂つ䛜䜣⣽⬊㑅ᢥⓗ⣽⬊ෆᑟධ䝅䝇䝔䝮䛾ᵓ⠏㻌 㻌 ᡃ䚻䛿⒴⣽⬊≉␗ⓗ䞉㑅ᢥⓗ᰾㓟་⸆䜢┠ᣦ䛧䚸䝨䝥䝏䝗䝸䝪᰾㓟䠄PRNA䠅䛸ྡ௜䛡䛯⣽⬊ෆ⎔ቃᛂ⟅ᛶ 䜢᭷䛩䜛᪂つேᕤ᰾㓟䜢タィ䞉ྜᡂ䛧䚸䛭䛾᭷⏝ᛶ䛻䛴䛔䛶ሗ࿌䛧䛶䛝䛯䚹䛥䜙䛻㧗ຠ⋡⣽⬊ᑟධ≉ᛶ௜୚ 䜢┠ᣦ䛧䚸⣽⬊ᑟධ䝰䝆䝳䞊䝹䛸䛧䛶䜸䝸䝂䜰䝹䜼䝙䞁 (Argn)䛸䛾」ྜ໬䜢᳨ウ䛧䚸Argnᑟධ䛜ᴟ䜑䛶᭷ຠ䛺 ᪉ἲㄽ䛷䛒䜛஦䜢ぢฟ䛧䛯䚹≉䛻㧗⣽⬊ᑟධ≉ᛶ䛸ప⣽⬊ẘᛶ䛸䛔䛖┦཯䛩䜛≉ᛶ⋓ᚓ䛻䛿䚸8ṧᇶ䛾䜰䝹䜼 䝙䞁ᑟධ䛜᭱㐺䛷䛒䜛䛣䛸䜒ሗ࿌䛧䛯䚹ᮏ◊✲䛷䛿䚸䜘䜚Ᏻ඲䞉Ᏻᚰ䛺⒴⣽⬊㑅ᢥⓗ᰾㓟་⸆䝅䝇䝔䝮㛤Ⓨ䜢 ┠ᣦ䛧䚸䜸䝸䝂䜰䝹䜼䝙䞁㒊఩䜈䛾⒴⣽⬊⭷㑅ᢥᛶ௜୚䜢᳨ウ䛧䛯䚹ලయⓗ䛻䛿䚸⒴⣽⬊㏆ഐ䛷≉␗ⓗ䛻㧗 㢖ᗘⓎ⌧䛜ሗ࿌䛥䜜䚸⒴䛾ᾐ₶䜔㌿⛣䜈䛾㛵୚䛜ሗ࿌䛥䜜䛶䛔䜛Matrix Metalloproteinase-9(MMP-9)1䛻╔┠ 䛧䚸䜰䝹䜼䝙䞁㒊఩䛻MMP-9ㄆ㆑㓄ิ䜢௓䛧ඃ䜜䛯⾑୰␃ᛶ䜢♧䛩䝫䝸䜶䝏䝺䞁䜾䝸䝁䌦䝹(PEG)䜢ᑟධ䛧䚸 MMP-9䛜䜒䛴䝥䝻䝔䜰䞊䝊άᛶ䜢ά⏝䛧䛯⒴⣽⬊㑅ᢥⓗ⣽⬊⭷㏱㐣ᶵ⬟䜢᭷䛩䜛PRNA㛤Ⓨ䛻ྲྀ⤌䜣䛰䚹 㻌 MMP-9 䛾ᇶ㉁㓄ิ䛸䛧䛶䚸≉␗ᛶ䛾㧗䛔㓄ิ䛸䛧䛶ሗ࿌䛥䜜䛶䛔䜛 7 ṧᇶ䝨䝥䝏䝗䠄PLGLRSK䠅䜢㑅ᢥ䛧䚸 N ᮎ䜎䛯䛿 C ᮎ➃䜢⺯ගಟ㣭䛧䛯䝨䝥䝏䝗䜢ྜᡂ䛧䚸MMP-9 䛻䜘䜛≉␗ⓗษ᩿≉ᛶ䜢᳨ウ䛧䛯䚹ᇶ㉁䝨䝥䝏. 2. 䝗䛾ྜᡂ䛻䛿䚸Fmoc ᅛ┦ἲ䜢㐺⏝䛧䚸㏫┦ HPLC 䜢⏝䛔䛶༢㞳⢭〇ᚋ䚸ESI-TOF-MS 䛻䜘䜚ྠᐃ䛧䛯䚹 ㏿ᗘ ㄽゎᯒ䛚䜘䜃ษ᩿఩⨨䛾ྠᐃ䜢⾜䛳䛯⤖ᯝ䚸ᇶ㉁䝨䝥䝏䝗䛿 N ᮎ䜎䛯䛿 C ᮎಟ㣭䛻㛵䜟䜙䛪 G 䛸 L 㛫䛷㑅 ᢥⓗ䛻ษ᩿䜢ཷ䛡䜛䛣䛸䛜᫂䜙䛛䛻䛺䛳䛯䚹 㻌 ḟ䛻䚸ᇶ㉁䝨䝥䝏䝗䜢௓䛧䛶䝫䝸䜶䝏䝺䞁䜾䝸䝁䞊䝹䠄PEG䠅䠄ᖹᆒศᏊ㔞 5000䠅䜢ᑟධ䛧䛯䜰䝹䜼䝙䞁䜢 8 ṧ ᇶ᭷䛩䜛 PEG ಟ㣭䝨䝥䝏䝗䛾タィ䞉ྜᡂ䜢⾜䛳䛯䚹䛣䛾 PEG ಟ㣭䝨䝥䝏䝗䛿䚸PEG ಟ㣭๓䛻ẚ㍑䛧཯ᛂ᫬㛫 䛾㐜ᘏ䛿ほ 䛥䜜䛯䜒䛾䛾䚸MMP-9 ษ᩿䛻క䛖 PEG 㒊఩䛾ゎ㞳䛜ྍ⬟䛷䛒䜛䛣䛸䛜᫂䜙䛛䛻䛺䛳䛯䚹䛣䛾⤖ ᯝ䜢㋃䜎䛘䚸Hela ⣽⬊䜢⏝䛔ษ᩿๓ᚋ䛷䛾⣽⬊⭷㏱㐣ᛶ䜢᳨ウ䛧䛯䚹ษ᩿๓䛾 PEG ಟ㣭䝨䝥䝏䝗䛿⣽⬊⭷ ㏱㐣ᛶ䛜㢧ⴭ䛻ᢚไ䛥䜜䛯䛾䛻ᑐ䛧䚸ษ᩿ᚋ䛾䝰䝕䝹䝨䝥䝏䝗䛿ຠᯝⓗ䛺⣽⬊ᑟධ䛜ほ 䛥䜜䛯䚹 ௨ୖ䛾⤖ᯝ䛛䜙䚸MMP-9 䛾Ⓨ⌧䛧䛶䛔䜛 ⒴⣽⬊㑅ᢥⓗ䛺⣽⬊⭷㏱㐣ᛶไᚚ䛻ᡂຌ䛧 䛯䚹ᮏ⣔䜢⏝䛔䜛䛣䛸䛻䜘䜚⣽⬊ᑟධẁ㝵䛻 䛚䛔䛶䜒⒴⣽⬊㑅ᢥᛶ䜢᭷䛩䜛ඃ䜜䛯䝕䝸 䝞䝸䞊䝅䝇䝔䝮๰〇䛜ᮇᚅ䛥䜜䜛䚹⌧ᅾᮾி ་⛉ṑ⛉኱ᶓ⏣㝯ᚨඛ⏕䛾༠ຊ䜢ᚓ䛶䚸⣽⬊ 䛚䜘䜃ື≀䝺䝧䝹䛷䛾᰾㓟་⸆ຠᯝ䛻䛴䛔 䛶ヲ⣽䛻᳨ウ䛧䛶䛔䜛䚹 (1) C. Gialeli et al, FEBS J. 2011, 278, 16–27 (2) Y. Huang et al, Biopolymers, 2013, 100, 790–795..

(7) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌ 研究活動報告. 㻞㻚㻌 㻌 ⿵ᅉᏊ㛫䜢ඹ᭷⤖ྜ䛻䜘䛳䛶㐃⤖䛧䛯ேᕤ㓝⣲」ྜయᵓ⠏䛻㛵䛩䜛◊✲㻌 ⏕యෆ䛷䛿䚸」ᩘ䛾㓝⣲䛜㐃⥆ⓗ䛛䛴༠ዌⓗ䛻ᇶ㉁ศᏊ䛻స⏝䛩䜛䛣䛸䛻䜘䛳䛶༢⊂䛷䛿㐩ᡂᅔ㞴䛺ከẁ 㝵ゐ፹཯ᛂ䜔䜹䝇䜿䞊䝗㐣⛬䜢ྍ⬟䛸䛧䛶䛔䜛䚹ኳ↛䛷䛿䚸≉ᐃ⣽⬊ෆ䜸䝹䜺䝛䝷䜈䛾ᒁᅾ໬䛻䜘䜛ᒁᡤ㓝⣲ ⃰ᗘ䛾ྥୖ䜔䚸≉␗ⓗ䝍䞁䝟䜽㉁㛫┦஫స⏝䛻ᇶ䛵䛟㉸ศᏊ」ྜయᙧᡂ䛻䜘䛳䛶䚸ᇶ㉁䛾άᛶ୰ᚰ㛫⛣ື᫬ 䛻䛚䛡䜛䝞䝹䜽⁐ᾮ୰䜈䛾ᣑᩓ䜔୙Ᏻᐃ୰㛫య䛾ศゎ䜢᭱ᑠ㝈䛻ᢚไ䛧䚸ຠ⋡ⓗ䛛䛴㐃⥆ⓗ䛺ከẁ㝵ゐ፹ ཯ᛂ䛻䜘䜛⏕⌮άᛶ≀㉁䛾๰〇䜢ᐇ⌧䛧䛶䛔䜛䚹ᚑ䛳䛶䚸⏕≀䛾ኳ↛㓝⣲䛜♧䛩ຠ⋡ⓗ䝅䞊䜿䞁䝅䝱䝹ゐ፹཯ ᛂ䜢䚸௵ព䛾㓝⣲䜢⤌䜏ྜ䜟䛫䛶ேᕤⓗ䛛䛴⮬ᅾ䛻ᐇ⌧ฟ᮶䜜䜀䚸⏕యෆ䝯䝍䝪䝸䝑䜽⣔䛾ά⏝䛷䛿ྜᡂᅔ㞴 䛺᪂つᶵ⬟ᛶ໬ྜ≀䛾ㄪ〇䜔䚸⎔ቃㄪ࿴ᆺゐ፹཯ᛂ䜈䛾ᛂ⏝䛜ྍ⬟䛸䛺䜛䜒䛾䛸⪃䛘䜙䜜䜛䚹௨ୖ䛾⫼ᬒ䜢 ㋃䜎䛘䛶䚸ᮏ◊✲䛷䛿䚸஧䛴䛾␗䛺䜛㓝⣲άᛶ୰ᚰ㛫䛾㊥㞳䜔㓄ྥ➼䜢ྵ䜑䛯✵㛫㓄⨨ไᚚ䜔≉␗ⓗ┦஫ ศᏊㄆ㆑䛻䜘䛳䛶䚸ຠ⋡ⓗ䛻ከẁ㝵㐃⥆཯ᛂ䜢ྍ⬟䛸䛩䜛䝍䞁䝟䜽㉁ᆺ㧗ᛶ⬟ゐ፹䛾㛤Ⓨ䜢┠ⓗ䛸䛧䛯䚹ලయ ⓗ䛻䛿䚸䝣䝷䝪䝍䞁䝟䜽㉁䛷䛒䜛䜾䝹䝁䞊䝇䜸䜻䝅䝎䞊䝊㻔㻳㻻㼤㻕䜔䝦䝮䝍䞁䝟䜽㉁䛷䛒䜛 㻴㼛㼞㼟㼑㼞㼍㼐㼕㼟㼔㻌 㻼㼑㼞㼛㼤㼕㼐㼍㼟㼑㻌 㻔㻴㻾㻼㻕➼䛾⿵ᅉᏊྵ᭷㓝⣲䛻╔┠䛧䚸䛭䜜䜙䛾άᛶ୰ᚰ䛻Ꮡᅾ䛩䜛 㻲㼘㼍㼢㼕㼚㻌㻭㼐㼑㼚㼕㼚㼑㻌㻰㼕㼚㼡㼏㼘㼑㼛㼠㼕㼐㼑㻌㻔㻲㻭㻰㻕䛸䝦䝮䝁 䝣䜯䜽䝍䞊䜢ඹ᭷⤖ྜ䜢௓䛧䛶㐃⤖䛧䛯ேᕤ⿵ᅉᏊ」ྜయタィ䛸ྜᡂ䜢ヨ䜏䛯䚹⿵ᅉᏊ」ྜయ䛾タィ䛻䛒䛯䛳 䛶䛿䚸ィ⟬ᶵ໬Ꮫⓗᡭἲ䜢⏝䛔䜛䛣䛸䛷஧䛴䛾⿵ᅉᏊ䜢⤖䜆䝸䞁䜹䞊䛾㛗䛥䜔ᑟධ఩⨨䛾᭱㐺໬䜢ᅗ䛳䛯䚹௒ ᚋ䚸䜰䝫䝍䞁䝟䜽㉁䛸䛾෌ᵓᡂ䜢⾜䛖䛣䛸䛷䚸䝍䞁䝟䜽㉁άᛶ୰ᚰ㛫䛾㊥㞳䜔㓄ྥ䜢ྵ䜑䛯✵㛫㓄⨨ไᚚ䛸㧗ຠ ⋡䝅䞊䜿䞁䝅䝱䝹ゐ፹཯ᛂ⣔䛾ᐇ⌧䜢┠ᣦ䛩䚹㻌 3䠊⏕య㧗ศᏊ䛾ືⓗᣲື䛾ゎᯒ䜢┠ᣦ䛧䛯㧗ឤᗘ䞉㧗᫬㛫᫬㛫ศゎ෇஧Ⰽ ᐃἲ䠄CD䠅䛾㛤Ⓨ䛸ᛂ⏝ ᐇ㦂䝟䝷䝯䞊䝍䛷䛒䜛೫ගᏊ᪉఩ゅ䜢⢭ᐦ䛻䝁䞁䝖䝻䞊䝹䛩䜛䛣䛸䛷䚸ಙྕᙉᗘ䛾ぢ䛛䛡ୖ䛾ቑ኱䚸䛩䛺䜟䛱 CD 䛾㧗ឤᗘ᳨ฟ䛜ྍ⬟䛻䛺䜛䛣䛸䜢ᐇド䛧䛶䛝䛯䚹ᮏᖺᗘ䛿䚸⏕య㧗ศᏊ䛻䛚䛡䜛ᵓ㐀ኚ໬䛾䝰䝕䝹໬ྜ≀ 䛸䛧䛶䚸ㄏ㉳Ⰽ⣲ศᏊ䛜䝅䜽䝻䝕䜻䝇䝖䝸䞁䛻ໟᦤ䛥䜜䛯㘒య䛾ືⓗᣲື䛻↔Ⅼ䜢䛒䛶䛯◊✲䜢⾜䛳䛯䚹䝅䜽䝻䝕 䜻䝇䝖䝸䞁䛻ໟᦤ䛥䜜䛯Ⰽ⣲䛜䚸ගບ㉳䛻క䛔ゎ㞳䛩䜛䛸䛔䛖⌧㇟䛜䛔䛟䛴䛛ሗ࿌䛥䜜䛶䛔䜛䛜䚸䛭䜜䜙䛾ሗ࿌ 䛿ගບ㉳≧ែ䛾྾཰䝇䝨䜽䝖䝹༶䛱㐣Ώ྾཰䝇䝨䜽䝖䝹䛾ᚤᑠ䛺ኚ໬㔞䛛䜙㆟ㄽ䛥䜜䛶䛝䛯䚹ᡃ䚻䛿䚸䝅䜽䝻䝕 䜻䝇䝖䝸䞁䛻ໟᦤ䛥䜜䜛䛣䛸䛷Ⰽ⣲䛾྾཰ᖏ䛻ㄏ㉳ CD 䛜⌧䜜䜛䛣䛸䛻╔┠䛧䚸ㄏ㉳ CD 䛾ᙉᙅ䛛䜙ໟᦤ㘒య䛾ᙧ ᡂ䇷ゎ㞳⌧㇟䜢ᤊ䛘䜛䛣䛸䛜ྍ⬟䛷䛒䜝䛖䛸⪃䛘䛯䚹ᐇ㦂䛿䚸䝯䝏䝹䜸䝺䞁䝆䜢䃒-䝅䜽䝻䝕䜻䝇䝖䝸䞁䛻ໟᦤ䛥䛫 䛯ໟᦤ㘒య䜢⏝䛔䛶⾜䛳䛯䚹䝯䝏䝹䜸䝺䞁䝆䛿䚸ẚ㍑ⓗ㧗⃰ᗘ᮲௳ୗ䛷䚸䃒-䝅䜽䝻䝕䜻䝇䝖䝸䞁䛸䠎䠖䠎㘒య䜢ᙧ ᡂ䛩䜛䛣䛸䛜▱䜙䜜䛶䛔䜛䚹㐣Ώ྾཰ ᐃ䛻䜘䜛ணഛⓗ䛺⪃ᐹ䛷䛿䚸䝯䝏䝹䜸䝺䞁䝆䜢ගບ㉳䛩䜛䛣䛸䛻䜘䜚䚸䝬 䜲䜽䝻⛊䛾᫬㛫䝇䜿䞊䝹䛻䛚䛔䛶䝯䝏䝹䜸䝺䞁䝆䛜䃒-䝅䜽䝻䝕䜻䝇䝖䝸䞁䛛䜙ᨺฟ䛥䜜䜛䛣䛸䜢♧၀䛩䜛䝕䞊䝍䛜 ᚓ䜙䜜䛯䚹䛣䛾᳨ウ⤖ᯝ䛿䚸䝬䜲䜽䝻⛊䛾᫬㛫ᛂ⟅⬟䜢♧䛩᫬㛫ศゎ CD ᐃ䛻䜘䜚䚸ᇶᗏ≧ែ䛾䝯䝏䝹䜸䝺䞁 䝆䛜♧䛩ㄏ㉳ CD 䛾ῶᑡ䛸ᑐᛂ䛧䛶䛔䜛䛣䛸䛛䜙⿬௜䛡䜙䜜䛯䚹ᚓ䜙䜜䛯㐣Ώ྾཰䛚䜘䜃㐣Ώ෇஧Ⰽᛶ䛾ᐃ㔞 ⓗ䛺᳨ウ䛛䜙䚸ගບ㉳䛻క䛖ゎ㞳䛾཰⋡䛿 0.8 ⛬ᗘ䛸㧗ຠ⋡䛷䛒䜚䚸௒ᚋ䚸ග䝇䜲䝑䝏➼䜈䛾ᛂ⏝䛜ᮇᚅ䛷䛝 䜛䚹䛭䛾䜋䛛䛾䝖䝢䝑䜽䛸䛧䛶䚸[6]䝦䝸䝉䞁ศᏊ䛾ᐊ ⁐ᾮ୰䛻䛚䛡䜛ບ㉳୕㔜㡯≧ែ䛾෇஧Ⰽᛶ ᐃ䛻ᡂຌ 䛩䜛䛺䛹䚸᫬㛫ศゎ CD ᐃἲ䛾ᛂ⏝䛻ྥ䛡䛯ᇶ♏ⓗ䛺◊✲䜢⾜䛳䛯䚹 䜎䛯䚸ඹྠ◊✲ᣐⅬ䛻䜘䜛ຓᡂ䜢ཷ䛡ከᩘ䛾ඹྠ◊✲䜢⾜䛳䛯䚹䜻䝷䝹䛺໬ྜ≀䛷䛒䜛䝦䝸䝉䞁䛾ගᏛ≉ᛶ 䜢䝁䞁䝖䝻䞊䝹䛩䜛䛯䜑䛾⨨᥮ᇶᑟධἲ䛻㛵䛩䜛◊✲䚸䛭䛧䛶୍㔜㡯ບ㉳Ꮚศ⿣䜢฼⏝䛧䛯㧗ຠ⋡୕㔜㡯ບ㉳ Ꮚ⏕ᡂ䛻㛵䛩䜛◊✲䜢៞ᛂ኱Ꮫ䛾⩚᭯㒊෸ᩍᤵ䛸ඹྠ䛧䛶⾜䛳䛯 1䚸2䚸3䚸4䚹 1. 2. 3. 4.. Horinouchi, H.; Sakai, H.; Araki, Y.; Sakanoue, T.; Takenobu, T.; Wada, T.; Tkachenko, N. V.; Hasobe, T. Chemistry - A European Journal. 2016, 22, 9631-9641. Yamamoto, Y.; Sakai, H.; Yuasa, J.; Araki, Y.; Wada, T.; Sakanoue, T.; Takenobu, T.; Kawai, T.; Hasobe, T. Journal of Physical Chemistry C. 2016, 120, 7421-7427. Sakuma, T.; Sakai, H.; Araki, Y.; Wada, T.; Hasobe, T. Physical Chemistry Chemical Physics. 2016, 18, 5453-5463. Sakuma, T.; Sakai, H.; Araki, Y.; Mori, T.; Wada, T.; Tkachenko, N. V.; Hasobe, T. Journal of Physical Chemistry A. 2016, 120, 1867-1875.. 䜎䛯䚸ඹྠ◊✲ᣐⅬ䛻䜘䜛ຓᡂ䜢ཷ䛡ከᩘ䛾ඹྠ◊✲䜢⾜䛳䛯䚹≉䛻ᡃ䚻䛾ບ㉳≧ែᣲືゎᯒᢏ⾡䚸㉸ ศᏊග୙ᩧ໬Ꮫ◊✲䛸䛾⼥ྜ䛻䜘䜚ྡ኱ὸ἟ᩍᤵ䚸ᶔ⏣෸ᩍᤵ䛸ேᕤ᰾㓟䛾䚸㜰኱⏘㐃ᮏ㒊すᔱඛ⏕䛸⺮ ⓑ㉁䜢୙ᩧ཯ᛂሙ䛸䛧䛯◊✲䚸៞ᛂ኱⩚᭯㒊෸ᩍᤵ䛸䝦䝸䝉䞁⣔䜻䝷䝹ศᏊ⣔ᵓ⠏䛸䛾≉ᛶゎᯒ䚸ṑᏛ㒊ව ᖹ༤ኈ䛸䛾▱ぬ㐣ᩄ⑕἞⒪⸆䜢┠ᣦ䛧䛯ṑ⛉ᮦᩱ᥈⣴䛺䛹᥎㐍䛧䚸 ከ䛟䛾ᡂᯝ䜢ୖ䛢䜛䛣䛸䛜ฟ᮶䛯䚹. 4.

(8) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌ 研究活動報告. 5. ࠙◊✲άືሗ࿌ࠚ㻌. ⏕࿨㢮ఝᶵ⬟໬Ꮫ◊✲ศ㔝䠄2016䠊1䡚2016䠊12䠅㻌. ᩍ ෸ ᩍ ຓ ኱ Ꮫ 㝔. ᤵ㸸࿴⏣ ೺ᙪ(ව௵) ᤵ㸸⛅ᒣ බ⏨ ᩍ㸸Ᏹ஭ ⨾✑Ꮚ ⏕㸸Adam Wawro䠄2016䠊1䡚9䠅,. Rui Li䠄2016䠊1䡚9䠅. 㻌. ᮏ◊✲ศ㔝䛷䛿䚸⏕యᶵ⬟ᛶศᏊ䛷䛒䜛䝍䞁䝟䜽㉁䜢ᇶ┙䛸䛧䚸㑇ఏᏊᕤᏛⓗᡭἲ䚸᭷ᶵྜᡂ໬Ꮫⓗᡭἲ 䜢⤌䜏ྜ䜟䛫䜛䛣䛸䛷⏕యศᏊ䛸ேᕤศᏊ䛾฼Ⅼ䜢ྲྀ䜚ධ䜜䛯䝴䝙䞊䜽䛺ᶵ⬟䜢᭷䛩䜛ศᏊ䚸䛒䜛䛔䛿䛭䛾ᶵ ⬟䜢⮬⏤⮬ᅾ䛻ไᚚ䛷䛝䜛ேᕤศᏊ䛾タィ䛸ᵓ⠏䜢⾜䛳䛶䛔䜛䚹䜎䛯䚸ගᶵ⬟Ⓨ⌧䛻㔜せ䛺㟁Ꮚ䜔䜶䝛䝹䜼 䞊⛣ື཯ᛂ䛾䝎䜲䝘䝭䝑䜽䝇䚸▷ᑑ࿨✀䛾㟁Ꮚ≧ែ䛸⎔ቃሙຠᯝ䜢ゎ᫂䛩䜛䛣䛸䜢┠ⓗ䛸䛧䛶䚸᫬㛫ศゎ䛚䜘 䜃䝟䝹䝇 㻱㻼㻾 ἲ䚸☢ሙຠᯝ䛾ほ 䛻ຍ䛘䛶᪂つ䛾䝟䝹䝇ศගᡭἲ䛾㛤Ⓨ䛻ྥ䛡䛯◊✲άື䜢⾜䛳䛶䛔䜛䚹 2016 ᖺ䛾◊✲άື䛸䛧䛶䛿䚸௨ୗ䛾䜘䛖䛻ᴫᣓ䛥䜜䜛䚹 㻌 ගཷᐜ䝍䞁䝟䜽㉁ PYP 䜢⏝䛔䛯ගᛂ⟅ᛶ䝒䞊䝹䛾㛤Ⓨ. 1.. ⏕࿨άື䛾୺ᖿ䜢ᢸ䛖䝍䞁䝟䜽㉁䛿䚸㧗ᗘ䛺ᶵ⬟ᛶ䛻ຍ䛘䚸⏕యぶ࿴ᛶ䚸⏕ศゎᛶ䚸ศᏊ䝃䜲䝈䛺䛹䛾ほ Ⅼ䛛䜙ᖜᗈ䛔ศ㔝䛷䛾ᛂ⏝䛜ᮇᚅ䛥䜜䛶䛔䜛⏕యᮦᩱ䛾䜂䛸䛴䛷䛒䜛䚹ᙜ◊✲ᐊ䛷䛿䚸㯤Ⰽ䝤䝗䜴⌫⳦⏤᮶ Ꮝᙧᡂẘ⣲ alpha-hemolysin(Hla)䜢䛿䛨䜑✀䚻䛾䝰䝕䝹䝍䞁䝟䜽㉁䛻ᑐ䛧䛶ගཷᐜయ䛷䛒䜛 Photoactive yellow protein (PYP)䜢⼥ྜ䛩䜛䛣䛸䛷䝍䞁䝟䜽㉁䜢ᇶ┙䛸䛩䜛ගไᚚྍ⬟䛺ศᏊ䝒䞊䝹䛾㛤Ⓨ䜢┠ᣦ䛧䛶䛔 䜛䚹 䛣䜜䜎䛷㐍䜑䛶䛝䛯ගᛂ⟅ᛶຍỈศゎ㓝⣲䛾ᵓ⠏䛻㛵䛧䛶䛿䚸ᑐ㇟䛸䛩䜛㓝⣲䛾」ᩘ㒊఩䜈䛾 PYP 䛾⼥ ྜ䜢᳨ウ䛧䚸㑇ఏᏊᕤᏛⓗᡭἲ䜢⏝䛔䛶 PYP 㓄ิ䜢ᑟධᚋ䚸ྛ䚻䛾ᨵኚయ䛻䛴䛔䛶኱⭠⳦ BL21(DE3)䜢⏝ 䛔䛯኱㔞Ⓨ⌧䛚䜘䜃⢭〇⣔䛾ᵓ⠏䜢⾜䛳䛯䚹PYP ⼥ྜ䛻క䛔኱䛝䛟㓝⣲άᛶ䜢኱䛝䛟ᦆ䛺䛳䛯ᨵኚయ䜢㝖䛔 䛯ṧ䜚䛾⼥ྜయ䛻䛴䛔䛶䛿䚸PYP ᥋⥆௜㏆䛾䜰䝭䝜㓟㓄ิ䛾᭱㐺໬䜢ྜ䜟䛫䛶㐍䜑䛶䛚䜚䚸ᘬ䛝⥆䛝䚸ᐇ⏝໬ 䜢ぢᤣ䛘䛯䝺䝧䝹䛷䛾ගᛂ⟅ᛶ๰ฟ䛻ྥ䛡䛶᳨ウ䜢⾜䛳䛶䛔䜛䚹 䜎䛯䚸䛣䜜䜎䛷␗䛺䜛䝃䝤䝣䜯䝭䝸䞊䛾㓝⣲䛻㛵䛧䛶䛿䚸PYP ⼥ྜ䜢᪋ 䛩䛣䛸䛻䜘䛳䛶㓝⣲཯ᛂ䛾㏿ᗘ䝟䝷䝯䞊䝍䛻ᑐ䛧䛶㢧ⴭ䛺ගᛂ⟅ᛶ䜢ぢ ฟ䛩䛣䛸䛻ᡂຌ䛧䛶䛚䜚䚸ᮏᖺᗘ䛿⣽⬊ᛶ⢓⳦䜢⏝䛔䛯䜽䜸䝷䝮䝉䞁䝅 䞁䜾䛾ගไᚚ⬟䛻䛴䛔䛶ホ౯䜢⾜䛳䛯䚹Fig.1 䛻䛿䚸⣽⬊ᛶ⢓⳦㞟ྜయ 䛾㢧ᚤ㙾ほᐹ䛾⤖ᯝ䜢♧䛩䚹ᬯ᮲௳ୗ䛸ẚ㍑䛧䚸㟷Ⰽග↷ᑕୗ䛻䛚䛔 䛶䛿⣽⬊ᛶ⢓⳦䛾㞟ྜ䛜ᢚไ䛥䜜䚸኱䛝䛺㞟ྜయ䛾ᙧᡂ䛿ほᐹ䛥䜜䛺 䛛䛳䛯䚹ᐇ㝿䚸㟷Ⰽග↷ᑕୗ䛻䛚䛔䛶䛿㓝⣲άᛶ䛜㜼ᐖ䛥䜜䜛䛣䛸䛜ヨ 㦂⟶䝺䝧䝹䛷☜ㄆฟ᮶䛶䛔䜛䚹㞟ྜ㐣⛬䛷䛿䚸䝅䜾䝘䝹ఏ㐩≀㉁䛷䛒 䜛 cAMP 䛾ಶయ䛾࿘㎶䛻䛚䛡䜛⃰ᗘ䛜㔜せ䛺㘽䛸䛺䜛䛣䛸䛜ሗ࿌䛥䜜䛶 䛚䜚䚸cAMP 䛾ศゎ䛜ᢚไ䛥䜜䜛䛣䛸䛷ᒁᡤⓗ䛻 cAMP ⃰ᗘ䛜ቑ኱䛧䚸 ຠ⋡ⓗ䛺㞟ྜ䛜ጉ䛢䛥䜜䛯䛸⪃䛘䜙䜜䜛䚹䛣䜜䛻䛴䛔䛶䛿䚸ᘬ䛝⥆䛝䚸 ヲ⣽䛺ศᏊ≉ᛶゎᯒ䜢㐍䜑䛶䛚䜚䚸ගᛂ⟅ᛶศᏊ䝒䞊䝹䜈䛾Ⓨᒎ䛜ᮇ ᚅ䛥䜜䜛䚹. Fig.1 ⣽⬊ᛶ⢓⳦䛾㞟ྜホ౯ (a) ᬯ᮲௳ୗ (b)㟷Ⰽග↷ᑕୗ.

(9) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌ 研究活動報告. 2.. ගᶵ⬟Ⓨ⌧䛻㛵୚䛩䜛㐣⛬䛾㟁Ꮚ䝇䝢䞁ග໬Ꮫ 㟁Ⲵศ㞳䛸෌⤖ྜ㐣⛬䛿ග཯ᛂึᮇ㐣⛬䜢⌮ゎ䛩䜛ୖ䛷㔜せ䛷䛒䜛䛸䛸䜒䛻䚸ศᏊ⣲Ꮚ䜔ኴ㝧ග䜶䝛䝹. 䜼䞊ኚ᥮䛸䛾㛵㐃䛷ᗈ䛟㛵ᚰ䛾ᡶ䜟䜜䛶䛔䜛◊✲ᑐ㇟䛸䛧䛶䛒䜛䚹䜎䛯䚸㟁Ꮚ䛾㟁Ⲵ䜀䛛䜚䛷䛺䛟䝇䝢䞁䛾ᛶ㉁ 䜢฼⏝䛧䛯ᛂ⏝䛻䛴䛔䛶㛵ᚰ䛜ᗈ䛜䛳䛶䛚䜚䚸䛣䛾Ⅼ䛻╔┠䛧䛯ගᶵ⬟ᛶศᏊ䛾ศᏊタィ䛜㐍䜑䜙䜜䛶䛔䜛䚹 䛭䛾㝿䛻䚸㟁Ⲵ䠄䜎䛯䛿㟁Ꮚ䠅㍺㏦䛸ྠᵝ䛻䚸㟁Ꮚ䝇䝢䞁䛜㛵䜟䜛㐣⛬䛻䛴䛔䛶䛾ᇶᮏⓗ䛺▱ぢ䛾⵳✚䛜㔜せ 䛺ᣦ㔪䜢ᥦ౪䛩䜛䜒䛾䛸⪃䛘䜙䜜䛶䛔䜛䚹䛣䜜䜎䛷㐍䜑䛶䛝䛯ගᶵ⬟Ⓨ⌧䛻㛵୚䛩䜛ᖖ☢ᛶάᛶ✀䛾㟁Ꮚᵓ 㐀䜢䚸㟁Ꮚ䝇䝢䞁䜢䝥䝻䞊䝤䛸䛧䛶᫂䜙䛛䛻䛩䜛◊✲䜢㐍䜑䛶䛝䛯䚹㻌 ୍㐃䛾⣔⤫ⓗ䛻ศᏊタィ䛥䜜䛯㟁Ꮚ౪୚య䇲ཷᐜయ㐃⤖⣔䜢◊✲ᑐ㇟䛸䛧䛶⏝䛔䚸ບ㉳≧ែ䛚䜘䜃㟁Ⲵ ศ㞳≧ែ䛾㟁Ꮚ䝇䝢䞁໬Ꮫⓗ◊✲䛛䜙䚸䐟㔜ཎᏊ䠄Pt䠅䛾䝇䝢䞁㌶㐨┦஫స⏝䛾ᐤ୚䚸䐠㟁Ꮚ౪୚య䠉ཷᐜ య㛫䛾㓄ྥ䛸㊥㞳ไᚚ䚸䛚䜘䜃䐡㠀ᒁᅾ໬䛧䛯 hole 䛾Ꮡᅾᵝᘧ➼䛾ᅉᏊ䛻䛴䛔䛶᳨ウ䜢㐍䜑䛶䛝䛯䚹䛭䜜 䛮䜜䛾ᐤ୚䛻䛴䛔䛶ᩚ⌮䛧䚸㟁Ⲵศ㞳䛾㧗ຠ⋡໬䛸⤖ᯝ䛸䛧䛶⏕ᡂ䛩䜛㟁Ⲵศ㞳≧ែ䛾㛗ᑑ࿨໬䛾㐩ᡂ䛻 ྥ䛡䛯ᣦ㔪䜢☜❧䛩䜛䛣䛸䜢ヨ䜏䛶䛔䜛䚹䛣䛾୰䛷䚸᪂つ䛾ศᏊෆ㟁Ⲵศ㞳⣔䛾᥈⣴䛸ᨵⰋ䜢㐍䜑䚸ᯫᶫ㒊 䛾ᵓᡂཬ䜃 hole 䛾Ꮡᅾᵝᘧไᚚ䛸㟁Ꮚ䝇䝢䞁≧ែ㛫⦆࿴䛾ᅉᏊ䛻䛴䛔䛶᳨ウ䜢㐍䜑䛯䚹ᮏᖺᗘ䛿䚸≉䛻䚸 ᯫᶫ㒊䛾㔜ཎᏊ䛻䜘䜛ಟ㣭䛾ຠᯝ䛻䛴䛔䛶ゎ᫂䛧䛯䚹㐣Ώⓗ䛺䝷䝆䜹䝹ᑐ䛾 EPR 䝇䝨䜽䝖䝹䛾ゎᯒ䛛䜙䚸䝷䝆 䜹䝹ᑐ䛾஺᥮┦஫స⏝䛾➢ྕ䜔኱䛝䛥䛿ಟ㣭䛾ຠᯝ䜢ཷ䛡䛺䛔䛣䛸䛜䜟䛛䜛䚹୍᪉䚸䛭䛾ຠᯝ䛿䚸㟁Ꮚ䝇䝢䞁 䝎䜲䝘䝭䜽䝇䛻㢧ⴭ䛻⾲䜜䜛䚹౛䛘䜀䚸ບ㉳≧ែ䛛䜙䛾཯ᛂ๓㥑య䛾䝇䝢䞁ከ㔜ᗘ䜢ኚ䛘䜛䜀䛛䜚䛷䛺䛟䚸⏕ᡂ 䛩䜛䝷䝆䜹䝹ᑐෆ䛷䛾䝇䝢䞁⦆࿴䚸ᑐ䛾ᑑ࿨➼䛻ᙳ㡪䜢୚䛘䜛䛣䛸䛜ヲ⣽䛻᫂䜙䛛䛻䛥䜜䛯䚹௒ᚋ䚸㟁Ⲵศ㞳 䛾㧗ຠ⋡໬䞉㛗ᑑ࿨໬䜢㐩ᡂ䛩䜛䛯䜑䛻䚸᭱䜒㐺ษ䛺ศᏊಟ㣭ᣦ㔪䜢☜❧䛩䜛䛣䛸䜢┠ᣦ䛧䛶㐍䜑䜛䚹. 3.. 䝘䝜䝃䜲䝈㉸ศᏊ⣔䛷䛾ග཯ᛂ໬Ꮫ. 㻌 䝘䝜䝃䜲䝈䛾ศᏊ⣔䛾ග཯ᛂ໬Ꮫ◊✲䜢㐍䜑䛯䚹 ᖹ㠃ᕧ኱䃟ศᏊ⣔䛜స䜚ฟ䛩୍ḟඖ✚ᒙᵓ㐀䛻╔┠䛧䚸䛣䛾✚ᒙᵓ㐀య䛾㟁Ⲵ㍺㏦፹య䛸䛧䛶䛾≉␗ᛶ 䜢᫂䜙䛛䛻䛩䜛◊✲䜢ィ⏬䚸ඹྠ◊✲䠄ᮾᕤ኱䞉⚟ᓥ◊䚸᪂₲኱䞉⏕㥖◊䠅䛸䛧䛶㐍䜑䜛䛣䛸䛸䛧䛯䚹㟁Ⲵ⏕ᡂ䚸 ㍺㏦㐣⛬䛷䛾㟁Ꮚ-䝩䞊䝹ᑐ䛾ᙺ๭䛻䛴䛔䛶䚸☢ሙຠᯝ䜢ྵ䜐㟁Ꮚ䝇䝢䞁䝎䜲䝘䝭䜽䝇◊✲䜢㐍䜑䛶䛔䜛䚹 䛣䛾◊✲䛸୪⾜䛧䛶䚸䛣䜜䜎䛷㐍䜑䛶䛝䛯 DNA ஧㔜㙐䛾ග㛤⿣䛻㛵୚䛩䜛䝷䝆䜹䝹ᑐ䛾㟁Ꮚ䝇䝢䞁ືຊᏛ ⓗ䛺◊✲䜢⫼ᬒ䛸䛧䛶䚸஧㔜㙐ษ᩿䛾ศᏊ⛉Ꮫⓗ䛺ᶵᵓ䛾ゎ᫂䛩䜛ඹྠ◊✲䛸䛧䛶ᒎ㛤䛧䛯䚹ඹྠ◊✲⪅䜙 䛾⌮ㄽⓗ䛺ྲྀ䜚ᢅ䛔䛻䜘䜚䚸▷㙐 DNA 䛾㙐㛤⿣ᶵᵓ䛜ヲ⣽䛻᫂䜙䛛䛻䛥䜜䛶䛝䛶䛔䜛䚹௒ᚋ䚸⎔ቃሙ䛾ຠᯝ 䛺䛹䜒⪃៖䛧䛯ゎᯒ䛜ィ⏬䛥䜜䛶䛔䜛䚹 䛥䜙䛻䚸᪂つ䛾ཎᏊෆໟ䝣䝷䞊䝺䞁䛾ᛶ㉁䛸㟁Ꮚᵓ㐀䜢ゎ᫂䛧䚸ගᶵ⬟ᛶᮦᩱ䜈䛾ᛂ⏝䜢ᒎᮃ䛧䛯◊✲䚸 ᾮᬗศᏊ䛾ගຎ໬ᶵᵓ䛻㛵୚䛩䜛ບ㉳≧ែ䛚䜘䜃ᖖ☢ᛶ✀䛾ᛶ㉁䛻䛴䛔䛶᫂䜙䛛䛻䛩䜛◊✲䜢ᘬ䛝⥆䛝㐍 䜑䛶䛔䜛䚹㻌. 6.

(10) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌ 研究活動報告. 7. ࠙◊✲άືሗ࿌ࠚ㻌 ᩍ. ⏕యศᏊᵓ㐀◊✲ศ㔝䠄2016䠊1䡚2016䠊12䠅㻌 ᤵ : ✄ⴥ ㅬḟ. ෸ ᩍ ᤵ : 㛛಴ ᗈ ຓ ᩍ : Ώ㒊 ⪽ ຓᩍ(ᖺಧไ)㸸஭ୖ 㐨㞝, ዟᮧ ṇᶞ, ኳ㈅ భኴ ◊✲ᨭ᥼⪅㸸୰ᮧ ⧊Ụ ◊✲⿵ຓဨ㸸ྜྷ⏣ ┤Ꮚ ᑠᯘ ⨾㤶 ᢏ ⾡ ⫋ ဨ㸸ᑠᕝ ⣪ஓຍ, ୖᯘ ࡉ࠾ࡾ ኱ Ꮫ 㝔 ⏕ : 㔠ᮧ 㐍࿃(D3), ⸨ᮏ ᣅᚿ(M1), ኱ᮧ ᆂ୍(M2), ኴᐓ ⤖(M2), ཎᒣ 㯞ዉ⨾(M2), ᰗ⃝ Ꮥಇ(M2), స⏣ ⳯ዉ⨾(M1), ᖹᒣ ༓ᑜ(M1), ⚟⏣ ὒ(M1), 㡑 㞷(M1) Ꮫ 㒊 Ꮫ ⏕㸸Elza F. Sofia(Ꮫ㒊 4 ᖺ) 㻌 ᮏ◊✲ศ㔝䛷䛿䚸⣽⬊䛻䛚䛡䜛䝍䞁䝟䜽㉁ရ㉁⟶⌮䝅䝇䝔䝮䛻䛴䛔䛶䚸ᵓ㐀⏕≀Ꮫ䚸⏕໬Ꮫ䚸䝥䝻䝔䜸䝭䜽䝇䚸 ⣽⬊⏕≀Ꮫ䛺䛹ከゅⓗ䜰䝥䝻䞊䝏䛻䜘䜚◊✲άື䜢⾜䛳䛶䛔䜛䚹2016 ᖺ䛾୺䛯䜛◊✲άື䛿䚸௨ୗ䛾䜘䛖䛻 ᴫᣓ䛥䜜䜛䚹. 㻌 㻝㻚㻌 䝠䝖⣽⬊䛾ศἪ⤒㊰䛻䛚䛡䜛 pH 䛸ள㖄䛻౫Ꮡ䛧䛯᪂䛯䛺䝍䞁䝟䜽㉁ရ㉁⟶⌮ᶵᵓ䛾ゎ᫂ ᪂つྜᡂ䛥䜜䛯ศἪ䝍䞁䝟䜽㉁䛿䚸ᑠ⬊యෆ⭍䛷ṇ䛧䛔❧యᵓ㐀䜢ᙧᡂ䛧䚸䝂䝹䝆య䜢⤒⏤䛧䛶⣽⬊እ䜈䛸 ㍺㏦䛥䜜䜛䚹୍᪉䚸㧗ḟᵓ㐀ୖᮍᡂ⇍䛺ศἪ䝍䞁䝟䜽㉁䜔䚸ᑠ⬊యᒁᅾ䝅䜾䝘䝹㓄ิ䜢䜒䛯䛺䛔ᑠ⬊యᒁᅾ 䝍䞁䝟䜽㉁(Ero1a䜔 Prx4 䛺䛹) 䛜䝂䝹䝆య䛻⛣⾜䛩䜛䛸䚸PDI 䝣䜯䝭䝸䞊䝍䞁䝟䜽㉁䛾 1 䛴䛷䛒䜛 ERp44 䛻ᤕ ᤊ䛥䜜䚸ᑠ⬊య䜈䛸㏫㍺㏦䛥䜜䜛䚹ERp44 䛿 a䚸b䚸b’䛾 3 䛴䛾䝏䜸䝺䝗䜻䝅䞁ᵝ䝗䝯䜲䞁䛸䚸C ᮎ䝔䞊䝹㡿ᇦ䛷ᵓ ᡂ䛥䜜䜛䚹䛣䜜䜎䛷䛾ᡃ䚻䛾◊✲䛛䜙䚸ERp44 䛿 pH ኚ໬䛻ᛂ⟅䛧䚸䜘䜚㓟ᛶ᮲௳䛾䝂䝹䝆య䛻䛚䛔䛶 C ᮎ䝔 䞊䝹䜢㛤䛝䚸ᇶ㉁䜢ຠ⋡Ⰻ䛟ᤕᤊ䛩䜛ᶵᵓ䜢ᥦၐ䛧䛯䚹⯆࿡῝䛔䛣䛸䛻ᡃ䚻䛿䚸ERp44 䛜 Zn2+䛸⤖ྜ䛩䜛䛣䛸䜒 Ⓨぢ䛧䛶䛚䜚䚸ᮏ◊✲ᐊ䛷᪂䛯䛻Ỵᐃ䛧䛯 ERp44 䛾㧗ศゎ⬟⤖ᬗᵓ㐀䛾᝟ሗ䛛䜙䚸C ᮎ䝔䞊䝹䛾᰿ඖ䛻Ꮡᅾ 䛩䜛䝠䝇䝏䝆䞁䝸䝑䝏㡿ᇦ䛜 Zn2+⤖ྜ㒊఩䛷䛒䜛䛸ண᝿䛧䛯䚹 䛭䛣䛷⢭〇䝍䞁䝟䜽㉁䜢⏝䛔䛯ᐃ㔞ⓗゎᯒ䜢㐙⾜䛧䚸Zn2+ῧຍ䛜 ERp44 䛸 Ero1 a 䛾ぶ࿴ᛶ䜢ቑ኱䛩䜛䛣䛸 䜢ぢฟ䛧䚸䜎䛯 Zn2+⤖ྜ䛻䜘䛳䛶 C ᮎ䝔䞊䝹䛜㛤䛟䛣䛸䛜ᙉ䛟♧၀䛥䜜䛯䚹䛥䜙䛻 2.45 Å ศゎ⬟䛻䛚䛔䛶 Zn2+ ⤖ྜᆺ ERp44 䛾⤖ᬗᵓ㐀ゎᯒ䛻ᡂຌ䛧䚸ண᝿㏻䜚䚸C ᮎ䝔䞊䝹᰿ඖ䛾䠏䛴䛾䝠䝇䝏䝆䞁䛜 Zn2+䛸㓄఩䛩䜛䛣䛸 䜢᫂䜙䛛䛻䛧䛯䚹௨ୖ䛾䛣䛸䛛䜙䚸Zn2+䛾⤖ྜ䛿 C ᮎ䝔䞊䝹䛾኱䛝䛺ᵓ㐀ኚ໬䜢ᘬ䛝㉳䛣䛧䚸ᇶ㉁⤖ྜ㒊఩䛜 㟢ฟ䛥䜜䜛䛣䛸䛜♧၀䛥䜜䛯䚹䜎䛯䚸Zn2+⤖ྜᆺ ERp44 䛿䚸b’䝗䝯䜲䞁୰䛾䠎䛴䛾䝠䝇䝏䝆䞁䛸 Zn2+䜢௓䛧䛯஧㔞 య䜢ᙧᡂ䛩䜛䛣䛸䛜᫂䜙䛛䛸䛺䛳䛯䚹䛣䜜䜙䛾⌧㇟䛿䚸䝠䝖ᇵ㣴⣽⬊ෆ䛷䜒෌⌧䛥䜜䚸Zn2+䛾Ḟஈ䜔෌ῧຍ䛻䜘 䜚䚸ERp44 䛸 Ero1 a 䛸䛾ぶ࿴ᛶ䛜䛭䜜䛮䜜ῶᑡ䚸ቑ኱䛧䛯䚹䛥䜙䛻ὀ┠䛩䜉䛝䛣䛸䛻䚸Zn2+䛾Ḟஈ䛿䚸ERp44 䜢䝂䝹䝆య䛻⵳✚䛥䛫䚸෌ῧຍ䛿 ERp44 䜢ᑠ⬊య䜈㏫㍺㏦䛥䛫䛯䚹⣽⬊ෆ䛻䛚䛔䛶䜒 ERp44 䛿஧㔞య䜢ᙧ ᡂ䛩䜛䛣䛸䜢ぢฟ䛧䚸୍᪉䛷 Zn2+⤖ྜ䛻㛵䜟䜛䝠䝇䝏䝆䞁䜢䜰䝷䝙䞁䛻⨨᥮䛧䛯ኚ␗య䛷䛿 Ero1 aศἪᢚไά ᛶ䛜᭷ព䛻పୗ䛧䛯䚹௨ୖ䛾䜘䛖䛻䚸ERp44 䛿 pH 䛻䜘䜛ไᚚ䛻ຍ䛘䛶䚸Zn2+䛸䛾⤖ྜ䜢௓䛧䛶䚸ᇶ㉁⤖ྜ⬟䜔 ⣽⬊ෆᒁᅾ䛜ไᚚ䛥䜜䜛䛣䛸䜢᫂䜙䛛䛻䛧䛯䚹䛣䜜䛿䚸䜋ஙື≀⣽⬊䛾ศἪ⤒㊰䛻䛚䛡䜛඲䛟᪂䛧䛔䝍䞁䝟䜽 ㉁ရ㉁⟶⌮ᶵᵓ䛾ゎ᫂䛷䛒䜛䚹㻌.

(11) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌ 研究活動報告. 㻞㻚ᑠ⬊య䜹䝹䝅䜴䝮䝫䞁䝥 SERCA2b, SERCA2a 䛾⤖ᬗᵓ㐀ゎᯒ䛸άᛶไᚚᶵᵓ ᑠ⬊య䛿⭷䝍䞁䝟䜽䜔ศἪ䝍䞁䝟䜽䛾ྜᡂ䛾ሙ䛷䛒䜚䚸䛭䛾㔜せ䛺⏕⌮ⓗᶵ⬟䛻䛚䛔䛶ᑠ⬊యෆ䛾㓟໬㑏 ඖ⎔ቃ䛚䜘䜃䜹䝹䝅䜴䝮⃰ᗘ䛜ᐦ᥋䛻㛵୚䛧䛶䛔䜛䚹ᑠ⬊య⭷୰䛻Ꮡᅾ䛩䜛䜹䝹䝅䜴䝮䝫䞁䝥 SERCA2b 䛿 ATP ౫Ꮡⓗ䛻⣽⬊㉁ෆ䛛䜙ᑠ⬊యෆ䜈䜹䝹䝅䜴䝮䜢㍺㏦䛩䜛ᶵ⬟䜢᭷䛧䚸ᑠ⬊యෆ䛾䜹䝹䝅䜴䝮⃰ᗘ䛾ᜏᖖ ᛶ⥔ᣢ䛻㛵䜟䜛䚹ᡃ䚻䛿ி㒔⏘ᴗ኱Ꮫ䛾Ọ⏣࿴ᏹᩍᤵ䛾䜾䝹䞊䝥䛸䛾ඹྠ◊✲䛾୍⎔䛸䛧䛶䚸ᑠ⬊యᒁᅾ 䝆䝇䝹䝣䜱䝗㑏ඖ㓝⣲ ERdj5 䛜 SERCA2b 䛾άᛶไᚚ䛻㛵䜟䜛䛣䛸䜢⢭〇䝍䞁䝟䜽㉁䜢⏝䛔䛯ゎᯒ䛻䜘䜚᫂䜙 䛛䛻䛧䚸䛭䛾ᡂᯝ䜢ㄽᩥ䛸䛧䛶Ⓨ⾲䛧䛯 1䚹䛭䛣䛷䚸䛥䜙䛺䜛ᑠ⬊యရ㉁⟶⌮ᶵᵓ䛾ゎ᫂䜢┠ᣦ䛧䚸SERCA2b 䛚 䜘䜃䛭䛾䜰䜲䝋䝣䜷䞊䝮䛷䛒䜚 TM11 䜢䜒䛯䛺䛔 SERCA2a 䛾ᵓ㐀ゎᯒ䛻ྲྀ䜚⤌䜣䛰䚹 ᩘᖺ䛻䜟䛯䜛䝃䞁䝥䝹ㄪ〇ἲ䛚䜘䜃⤖ᬗ໬᮲௳䛾᳨ウ䛾⤖ᯝ䚸ᡃ䚻䛿 3.3- 3.4 Å 䛾ศゎ⬟䛷㓟໬ᆺ䞉㑏ඖ ᆺ SERCA2b 䛚䜘䜃 SERCA2a 䛾⤖ᬗᵓ㐀ゎᯒ䛻ᡂຌ䛧䛯䚹㑏ඖᆺ SERCA2b 䛷䛿㓟໬ᆺ䛻ẚ䜉 TM11 ⏤ ᮶䛸ᛮ䜟䜜䜛㟁Ꮚᐦᗘ䛜䜘䜚ᙉ䛟ほ 䛥䜜䛯䚹୍᪉䛷䚸㑏ඖᆺ SERCA2b 䛻䛚䛔䛶䜹䝹䝅䜴䝮⤖ྜ㒊఩䛻䛒䜛 Ca2+ 䛾༨᭷⋡䛜ⴭ䛧䛟పୗ䛩䜛䛺䛹䚸㓟໬㑏ඖ౫Ꮡⓗ䛺ᕪ䛜ᵓ㐀ゎᯒ䛾⤖ᯝ䛛䜙᫂䜙䛛䛸䛺䛳䛯䚹䛥䜙䛻䚸 SERCA2b 䛾ᗄ䛴䛛䛾཯ᛂ୰㛫≧ែ䛾ᵓ㐀Ỵᐃ䜔䛥䜙䛺䜛ศゎ⬟䛾ྥୖ䜢┠ᣦ䛧䚸SERCA2b 䜢≉␗ⓗ䛻ㄆ ㆑䛩䜛䝰䝜䜽䝻䞊䝘䝹ᢠయ䛾䝇䜽䝸䞊䝙䞁䜾䛚䜘䜃ㄪ〇䜢᏶஢䛥䛫䛯䚹 ௨ୖ䛾ᵓ㐀⏕≀Ꮫⓗ䜰䝥䝻䞊䝏䛻ຍ 䛘 䚸SERCA2b 䛾ά ᛶ 䜢䜘 䜚⏕ ⌮ⓗ ᮲ ௳䛻㏆ 䛔 ᮲௳ ୗ䛷 ホ౯ 䛩䜛 䛯 䜑䚸 ෌ᵓ ᡂ 䝥䝻 䝔䜸䝸 䝫 䝋 䞊䝮 䜔 SERCA2b 㐣๫Ⓨ⌧⣽⬊䛛䜙ㄪᩚ䛧䛯䝭䜽䝻䝋䞊䝮䜢⏝䛔䛯ᐇ㦂⣔䜢ᵓ⠏䛧䛯䚹 1. Ushioda et al., Proc Natl Acad Sci U S A. (2016) 113(41):E6055-E6063. 㻟㻚㻌LDL ཷᐜయ䜢䝰䝕䝹ᇶ㉁䛻䜒䛱䛔䛯䚸ᑠ⬊యෆ䛻䛚䛡䜛䝍䞁䝟䜽㉁䛾䝣䜷䞊䝹䝕䜱䞁䜾ᶵᵓ䛾ゎᯒ 䝆䝇䝹䝣䜱䝗⤖ྜ䛾ᙧᡂ䛿䚸ከ䛟䛾⏕≀䛻䛚䛔䛶䚸ศἪ䝍䞁䝟䜽㉁䜔⭷䝍䞁䝟䜽㉁䛾❧యᵓ㐀ᙧᡂ䜔ᶵ⬟Ⓨ ⌧䛻㔜せ䛷䛒䜛䚹့ஙື≀⣽⬊䛾ᑠ⬊యෆ䛷䝆䝇䝹䝣䜱䝗⤖ྜ䛜ຠ⋡䜘䛟ᙧᡂ䛥䜜䛺䛔䛸⢾ᒀ⑓䜔㧗⬡⾑⑕ 䛾ཎᅉ䛻䛺䜚䛖䜛䚹䜘䛳䛶䚸䝆䝇䝹䝣䜱䝗⤖ྜ䛾ᙧᡂ䛾௙⤌䜏䜢▱䜛䛣䛸䛿㔜せ䛷䛒䜛䚹䛧䛛䛧䚸ᑠ⬊య୰䛻㏦䜚 ㎸䜎䜜䛶䛝䛯䝍䞁䝟䜽㉁䛾䝫䝸䝨䝥䝏䝗㙐䛻ᑐ䛧䛶䚸䛔䛴䚸䛹䛾㓝⣲䛜䚸䛹䛾䜘䛖䛻䛧䛶䝆䝇䝹䝣䜱䝗⤖ྜ䛜ᑟධ 䛥䜜䜛䛾䛛䛻䛴䛔䛶䛿䜋䛸䜣䛹ศ䛳䛶䛔䛺䛔䚹䛣䛾௙⤌䜏䜢ゎ᫂䛩䜛䛯䜑䛻䚸⚾䛯䛱䛿⾑ᾮ୰䛾 LDL ⢏Ꮚ䛾 ⣽⬊ෆ䜈䛾ྲྀ䜚㎸䜏䛻ᚲせ䛺⭷䝍䞁䝟䜽㉁䛷䛒䜛 LDL ཷᐜయ(LDLR)䜢䝰䝕䝹ᇶ㉁䛾୍䛴䛸䛧䛶⏝䛔䛯䚹䛺 䛚䚸LDLR 䛿ከᩘ䛾䝗䝯䜲䞁䛛䜙䛺䜛୍ᅇ⭷㈏㏻䝍䞁䝟䜽㉁䛷䛒䜚䚸LDLR ศᏊୖ䛻䝆䝇䝹䝣䜱䝗⤖ྜ䛿䚸7 ಶ䛾 R 䝗䝯䜲䞁䛸 3 ಶ䛾 EGF 䝗䝯䜲䞁䛾ྛ䚻䛻 3 ᮏ䛪䛴䚸ィ 30 ᮏᏑᅾ䛩䜛䚹 ௒ᅇᡃ䚻䛿䚸䝸䝪䝋䞊䝮ୖ䛷⩻ヂྜᡂ㏵ୖ䛛䛴ᑠ⬊యෆ䛷ఙ㛗䛩䜛 LDLR 䛾䝫䝸䝨䝥䝏䝗㙐䛻䝆䝇䝹䝣䜱䝗 ⤖ྜ䛜ᑟධ䛥䜜䜛㐣⛬䜢ほᐹ䛩䜛䛯䜑䛾⣔䜢ᵓ⠏䛩䜛䛣䛸䛻ᡂຌ䛧䛯䚹䛣䛾⣔䜢฼⏝䛩䜛䛣䛸䛻䜘䛳䛶䚸1) LDLR 䜈䛾䝆䝇䝹䝣䜱䝗⤖ྜ䛾ᑟධ䛿⩻ヂ䛸ඹᙺ䛧䛶㉳䛣䜚䛖䜛䛣䛸䚸2) ⩻ヂ䛸ඹᙺ䛧䛶㉳䛣䜛 LDLR 䛾㓟໬ⓗ 䛺䝣䜷䞊䝹䝕䜱䞁䜾཯ᛂ䛿䠏䛴䛾䝣䜵䞊䝈䛻ศ䛡䜙䜜䜛䛣䛸䚸3) 䛭䛾䛖䛱䛾➨䠍䛸➨䠎䛾䝣䜵䞊䝈䛿䚸LDLR 䛾 N ᮎ➃㏆ഐ䛻Ꮡᅾ䛩䜛䠓ಶ䛾 R 䝗䝯䜲䞁䛾䛭䜜䛮䜜䛻䝆䝇䝹䝣䜱䝗⤖ྜ䛜ᑟධ䛥䜜䜛཯ᛂ䜢䜅䛟䜐䜒䛾䛷䛒䜛䛣䛸 䜢᫂䜙䛛䛻䛧䛯䚹䛣䛾䜘䛖䛺ゎᯒ䛛䜙䚸ᑠ⬊యෆ䛻䛚䛡䜛䝍䞁䝟䜽㉁䛾䝣䜷䞊䝹䝕䜱䞁䜾཯ᛂ䛿䝸䝪䝋䞊䝮ୖ䛷䛾 䝨䝥䝏䝗䛾ఙ㛗཯ᛂ䛸ᙉ䛟ඹᙺ䛧䛶ẁ㝵ⓗ䛻㐍⾜䛧䛖䜛䛣䛸䛜ึ䜑䛶┤᥋ⓗ䛻♧䛥䜜䛯䚹 㻠㻚 ⮅≉␗ⓗ䛻Ⓨ⌧䛩䜛 PDI 䝣䜯䝭䝸䞊䝍䞁䝟䜽㉁䠄PDIp䠅䛾⏕⌮ⓗ䛺ᶵ⬟䛾ゎᯒ ့ஙື≀⣽⬊䛾ᑠ⬊యෆ䛻䛿䝆䝇䝹䝣䜱䝗⤖ྜᙧᡂ䛻㛵䜟䜛䛸ண᝿䛥䜜䜛 PDI 䝣䜯䝭䝸䞊䝍䞁䝟䜽㉁䛜 20 ✀ 㢮䜒Ꮡᅾ䛩䜛䚹䛭䛾䛖䛱䛾୍䛴䛷䛒䜛 PDIp 䛿䚸䜲䞁䝇䝸䞁䜔ᾘ໬㓝⣲䛺䛹⏕࿨䛻䛸䛳䛶㔜せ䛺䝍䞁䝟䜽㉁䜢኱ 㔞䛻⏘⏕䛩䜛⮅⮚䛻≉␗ⓗ䛻Ꮡᅾ䛩䜛䛣䛸䛜▱䜙䜜䛶䛔䛯䚹䛧䛛䛧䚸䛭䛾ᶵ⬟䛻䛴䛔䛶䛿䜋䛸䜣䛹䜟䛛䛳䛶䛔 䛺䛛䛳䛯䚹䛭䛣䛷⚾䛯䛱䛿䚸PDIp 䛾⏕⌮ⓗᶵ⬟䜢ゎ᫂䛩䜛䛯䜑䚸◊✲䜢㐍䜑䛯䚹䛣䜜䜎䛷䛻 PDIp 䛿⮅⮚䛰䛡 䛷䛺䛟⫶䚸ᑠ⭠䛺䛹ᾘ໬䛻㛵䜟䜛ჾᐁ䛻Ⓨ⌧䛧䛶䛚䜚䚸≉䛻⮅⮚䛾እศἪ⣽⬊䛷ከ䛔䛣䛸䜢ぢฟ䛧䛯䚹᭦䛻䚸 PDI 䝣䜯䝭䝸䞊䛻ᒓ䛩䜛㓝⣲䛜ᇶ㉁䛻స⏝䛩䜛㝿䛻䛿䚸㓝⣲䛸ᇶ㉁䛜ศᏊ㛫䛾䝆䝇䝹䝣䜱䝗⤖ྜ䛷㐃⤖䛧䛯」. 8.

(12) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌ 研究活動報告. 9. ྜయ䜢ᙧᡂ䛩䜛䛣䛸䜢฼⏝䛧䛶䚸PDIp 䛾ᇶ㉁ೃ⿵䝍䞁䝟䜽㉁䜢㉁㔞ศᯒ䛻䜘䛳䛶ከᩘྠᐃ䛧䛯䚹ᮏᖺᗘ䛿䚸䛣 䛾䜘䛖䛻ྠᐃ䛧䛯ᇶ㉁ೃ⿵䝍䞁䝟䜽㉁䛸 PDIp 䜢䝠䝖⏤᮶䛾ᇵ㣴⣽⬊ᰴ䛷䛒䜛 HeLa ⣽⬊୰䛷ඹⓎ⌧䛥䛫䜛䛣䛸 䛻䜘䛳䛶䚸PDIp 䛜䛣䜜䜙䛾䝍䞁䝟䜽㉁䛾⏕ྜᡂ䛻ᯝ䛯䛩ᙺ๭䜢ゎᯒ䛧䛯䚹ྠᐃ䛥䜜䛯ᇶ㉁ೃ⿵䝍䞁䝟䜽㉁䛾䛖 䛱䚸䜻䝰䝖䝸䝥䝅䞁 B 䛾ሙྜ䛻䛿 PDIp 䛾Ⓨ⌧㔞䛾ቑຍ䛻క䛔䚸⣽⬊୰䛻䛚䛡䜛䝍䞁䝟䜽㉁䛾⵳✚㔞䛾ቑ኱䛜 ぢ䜙䜜䛯䚹䜎䛯䚸䜶䝷䝇䝍䞊䝊䛾ሙྜ䛻䛿䚸ᇵ㣴⣽⬊୰䛷䜶䝷䝇䝍䞊䝊䜢ᙉⓎ⌧䛩䜛䛸䜶䝷䝇䝍䞊䝊䛿⣽⬊ෆ䛷 㧗ศᏊ㔞䛾จ㞟య䜢ᙧᡂ䛧䛯䛜䚸PDIp 䛾ඹⓎ⌧䛻䜘䜚䛣䛾จ㞟య䛿ῶᑡ䛧䚸䜶䝷䝇䝍䞊䝊༢㔞య䛾⣽⬊ෆ⵳ ✚㔞䛚䜘䜃ᇵᆅ୰䜈䛾ศἪ㔞䛜ቑ኱䛧䛯䚹䛣䛾䜘䛖䛺ほᐹ⤖ᯝ䛛䜙 PDIp 䛿ᾘ໬㓝⣲䛾⏕ྜᡂ䜢ಁ㐍䛧䛶䛔䜛 䛣䛸䛜ᙉ䛟♧၀䛥䜜䛯䚹 㻡㻚㻌 ↓⣽⬊ྜᡂ⣔䜢⏝䛔䛯᪂⏕㙐䝆䝇䝹䝣䜱䝗⤖ྜᙧᡂᶵᵓ䛾ゎ᫂ 䝸䝪䝋䞊䝮ୖ䛷⏕ྜᡂ䛥䜜䛯᪂⏕㙐(⭷䝍䞁䝟䜽㉁䜔ศἪ䝍䞁䝟䜽㉁)䛿䚸ᑠ⬊యෆ䛷䝆䝇䝹䝣䜱䝗⤖ྜᙧᡂ 䜢క䛖ᢡ䜚␚䜏䚸䛩䛺䜟䛱㓟໬ⓗ䝣䜷䞊䝹䝕䜱䞁䜾䜢⤒䛶ኳ↛䛾❧యᵓ㐀䜢⋓ᚓ䛩䜛䚹䛣䜜䜎䛷䛾◊✲䛻䜘䜚䚸 ᑠ⬊య䛻Ꮡᅾ䛩䜛 20 ✀㢮௨ୖ䛾 PDI family 䛜䛣䛾䝆䝇䝹䝣䜱䝗⤖ྜᙧᡂ䜢ಁ㐍䛧䛶䛔䜛䛣䛸䛜᫂䜙䛛䛸䛺䛳䛶 䛔䜛䚹䛧䛛䛧䛺䛜䜙䚸䛭䜜䜙 PDI family 䛜䛹䛾ẁ㝵(⩻ヂ㏵୰䚸䛒䜛䛔䛿⩻ヂᚋ)䛷䚸᪂⏕㙐䛾䝆䝇䝹䝣䜱䝗⤖ྜ ᙧᡂ䜢ゐ፹䛧䛶䛔䜛䛛䛿ᮍ䛰᫂䜙䛛䛷䛿䛺䛔䚹䛭䛣䛷 PDI family 䛻䜘䜛᪂⏕㙐䛾ศᏊㄆ㆑ᶵᵓཬ䜃䝆䝇䝹䝣䜱 䝗⤖ྜᙧᡂᶵᵓ䛾ゎ᫂䜢┠ᣦ䛧䛯䚹ᡃ䚻䛿↓⣽⬊䝍䞁䝟䜽㉁ྜᡂ⣔䛸䝠䝖⾑Ύ䜰䝹䝤䝭䞁(HSA)䛾 N ᮎ➃䛛䜙 ✀䚻䛾㛗䛥䛾䝁䞁䝇䝖䝷䜽䝖䜢⏝䛔䜛䛣䛸䛷䚸PDI 䜎䛯䛿 ERp46 䛻䜘䜛⩻ヂ㏵୰䛾 HSA 䛾䝆䝇䝹䝣䜱䝗⤖ྜᙧᡂ 䛾ほ 䛻ᡂຌ䛧䛯䚹䛭䛾⤖ᯝ䚸HAS 䛾䝸䝪䝋䞊䝮䛛䜙㟢ฟ䛧䛶䛔䜛㡿ᇦ䛾㛗䛥䜔⏝䛔䜛 PDI 䝣䜯䝭䝸䞊㓝⣲䛻䜘 䛳䛶᪂⏕㙐䛾䝆䝇䝹䝣䜱䝗⤖ྜᙧᡂ䛻᭷ព䛺ᕪ䛜䛒䜛䛣䛸䛜♧䛥䜜䛯䚹⌧ᅾ䚸PDI family 㓝⣲䛾᪂⏕㙐䜈䛾స ⏝ᶵᗎ䜢ヲ⣽䛻ㄪ䜉䜛䛯䜑䚸pro-BPTI 䛺䛹␗䛺䜛ᇶ㉁䛾⣔⤫ⓗ䛻㛗䛥䜢ኚ䛘䛯䝁䞁䝇䝖䝷䜽䝖䜢⏝䛔䛶䚸PDI family 䛻䜘䜛᪂⏕㙐䜈䛾䝆䝇䝹䝣䜱䝗⤖ྜᙧᡂ཯ᛂ䜢ゎᯒ䛧䛶䛔䜛䚹 㻢㻚㻌ERdj5 䛸 BiP 䛾ඹᙺ䛻䜘䜛䝆䝇䝹䝣䜱䝗⤖ྜ㑏ඖᶵᵓ ᑠ⬊యෆ䛷䝭䝇䝣䜷䞊䝹䝗䛧䛯䝍䞁䝟䜽㉁䛿ᑠ⬊య㛵㐃ศゎ(ERAD)䛻䜘䛳䛶㏿䜔䛛䛻ศゎ䛥䜜䚸䝍䞁䝟䜽㉁ 䛾ရ㉁䛜⟶⌮䛥䜜䜛䚹䛣䜜䜎䛷䛻㠀⢾㙐䝭䝇䝣䜷䞊䝹䝗䝍䞁䝟䜽㉁䛾ศゎ㐣⛬䛻䛚䛔䛶䚸BiP䛜䝭䝇䝣䜷䞊䝹䝗䝍 䞁䝟䜽㉁䜢ㄆ㆑䛧䚸ERdj5䛻ཷ䛡Ώ䛩䛣䛸䛷䚸ERdj5䛜䝭䝇䝣䜷䞊䝹䝗䝍䞁䝟䜽㉁ෆ䛾䝆䝇䝹䝣䜱䝗⤖ྜ䜢㑏ඖ䛩 䜛䛣䛸䛜ሗ࿌䛥䜜䛶䛔䜛(Ushioda, et al. Mol Biol Cell. 2013)䚹୍᪉䚸⢾㙐䝭䝇䝣䜷䞊䝹䝗䝍䞁䝟䜽㉁䛻䛴䛔䛶䚸᭱ ึ䛻EDEM1䛜䝭䝇䝣䜷䞊䝹䝗䝍䞁䝟䜽㉁䜢ㄆ㆑䛧ERdj5䛾Cᮎ➃䜽䝷䝇䝍䞊䛻⤖ྜ䛩䜛䛣䛸䛻䜘䜚䚸䝭䝇䝣䜷䞊䝹䝗 䝍䞁䝟䜽㉁䛾㠀ኳ↛ᆺ䛾䝆䝇䝹䝣䜱䝗⤖ྜ䛜ERdj5䛾Cᮎ➃䜽䝷䝇䝍䞊୰䛾άᛶ㒊఩䛻䜘䛳䛶㑏ඖ䛥䜜䜛䛣䛸䛜 ♧䛥䜜䛶䛔䜛(Hagiwara, et al. Mol. Cell 2011)䚹䛧䛛䛧䛺䛜䜙䚸BiP䜔EDEM1䛜䛹䛾䜘䛖䛻䝭䝇䝣䜷䞊䝹䝗䝍䞁䝟䜽 ㉁䜢ㄆ㆑䛧䚸䛹䛖䜔䛳䛶ERdj5䛻ཷ䛡Ώ䛩䛛䚸䛭䛧䛶ERdj5䛜䛹䛾䜘䛖䛺䝯䜹䝙䝈䝮䛷䝭䝇䝣䜷䞊䝹䝗䝍䞁䝟䜽㉁䛾 䝆䝇䝹䝣䜱䝗⤖ྜ䜢㑏ඖ䛩䜛䛛ᮍ䛰୙᫂䛺Ⅼ䛜ከ䛔䚹䛭䛣䛷ኳ↛ᵓ㐀䜢䜒䛴䝍䞁䝟䜽㉁䜔䝭䝇䝣䜷䞊䝹䝗䛧䛯䝍 䞁䝟䜽㉁䜢ᇶ㉁䛸䛧䛶⏝䛔䚸BiPᏑᅾୗ䛒䜛䛔䛿㠀Ꮡᅾୗ䛻䛚䛔䛶ERdj5䛻䜘䜛䝆䝇䝹䝣䜱䝗⤖ྜ䛾㑏ඖ䜢ㄪ䜉 䛯⤖ᯝ䚸BiP䛜ERdj5䛾䝆䝇䝹䝣䜱䝗⤖ྜ㑏ඖ⬟䜢ಁ㐍䛩䜛䛣䛸䛜᫂䜙䛛䛸䛺䛳䛯䚹୍᪉䚸ኚᛶᇶ㉁䜢౑⏝䛧䚸 BiPᏑᅾୗ䛻䛚䛔䛶ERdj5䛻䜘䜛ᇶ㉁䜈䛾䝆䝇䝹䝣䜱䝗⤖ྜᑟධ⬟䜢ホ౯䛧䛯⤖ᯝ䚸ERdj5䛻䜘䜛ᇶ㉁䜈䛾䝆䝇 䝹 䝣 䜱 䝗 ⤖ ྜ ᑟ ධ ⬟ 䛿 ௚ 䛾 PDI 䝣 䜯 䝭 䝸 䞊 䛸 ẚ 䜉 䚸 ⴭ 䛧 䛟 ప 䛛 䛳 䛯 䚹 ௨ ୖ 䛾 ⤖ ᯝ 䛛 䜙 䚸 ኱ ⭠ ⳦ 䛻 䛚 䛡 䜛 DsbA-DsbB 䝆䝇䝹䝣䜱䝗⤖ྜ㓟໬⤒㊰䛸DsbC-DsbD 䝆䝇䝹䝣䜱䝗⤖ྜ㑏ඖ⤒㊰䛸ྠᵝ䛻䚸့ஙື≀⣽⬊䛻 䛚䛡䜛ᑠ⬊య䛻䛚䛔䛶䜒䚸䝆䝇䝹䝣䜱䝗⤖ྜ䛾㓟໬⤒㊰䛸㑏ඖ⤒㊰䛜ศ᩿䛥䜜䜛䛣䛸䛜♧၀䛥䜜䛯䚹௒ᚋ䚸ከ ✀ከᵝ䛺ᇶ㉁䜢⏝䛔䜛䛣䛸䛷䚸ERdj5䛸BiP䛾ඹᙺ䛻䜘䜛䝆䝇䝹䝣䜱䝗⤖ྜ㑏ඖᶵᵓ䛾ᬑ㐢ᛶ䜢᫂䜙䛛䛻䛩䜛ண ᐃ䛷䛒䜛䚹.

(13) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌ 研究活動報告. ࠙◊✲άືሗ࿌ࠚ. 10. ⣽⬊ᶵ⬟ศᏊ໬Ꮫ◊✲ศ㔝㸦2016㸬1㹼2016㸬12㸧㻌 ᩍ ෸ ᩍ ຓ ᢏ⾡⫋. ᤵỈୖ㐍 2016 ᖺ 4 ᭶ࠥ㸧 ᤵᯇ஭ᩄ㧗 ᩍᑠ࿴⏣ಇ⾜ 2016 ᖺ 11 ᭶ࠥ

(14)  ဨ༓ᅜ཭Ꮚ. ◊ ✲ ᨭ ᥼ ဨబ⸨ᑑᏊ ᢏ ⾡ ᨭ ᥼ ဨ⠛ᓮ⋹㤶  ᮏ◊✲ศ㔝䛷䛿䚸ձ䝞䜲䜸䜲䝯䞊䝆䞁䜾䝥䝻䞊䝤䛾㛤Ⓨ䛸䜲䝯䞊䝆䞁䜾䛻ᇶ䛵䛟⏕࿨⌧㇟䛾ゎ᫂䚸ղ㔠ᒓ䝍 䞁䝟䜽㉁䛾ᵓ㐀䛸ᶵ⬟㛵䛩䜛◊✲䚸䛻኱ู䛥䜜䜛◊✲䜢⾜䛳䛶䛔䜛䚹2016 ᖺ䛾◊✲άື䛸䛧䛶䛿䚸ձ䛻䛴䛔䛶 䛿⣽⬊ෆ Mg2+ືែ䜢㛗᫬㛫ྍど໬䛩䜛పศᏊ-䝍䞁䝟䜽㉁䝝䜲䝤䝸䝑䝗⺯ග䝥䝻䞊䝤䛾㛤Ⓨ䛸䜰䝫䝖䞊䝅䝇䛻 䛚䛡䜛⣽⬊ෆ Mg2+ືែ䛾ゎᯒ䜢⾜䛳䛯䠄㡯┠ 1䠅䚹ղ䛻䛴䛔䛶䛿⑓ཎᛶ⣽⳦⏤᮶䛾᪂ᆺ䝦䝮ศゎ㓝⣲䚸䛚䜘 䜃ேᕤ㔠ᒓ㓝⣲䛾๰ฟ䛻㛵䛩䜛◊✲䜢୺䛻㐍䜑䛯䠄㡯┠ 2䠈3䠅䚹䛭䜜䜙䛾ᡂᯝ䛿௨ୗ䛾䜘䛖䛻ᴫᣓ䛥䜜䜛䚹 㻌. 1䠊 ⣽⬊ෆ Mg2+ືែ䜢ྍど໬䛩䜛⺯ග䝥䝻䞊䝤䛾㛤Ⓨ䛸䜰䝫䝖䞊䝅䝇䛻䛚䛡䜛⣽⬊ෆ Mg2+ືែ䛾ゎᯒ Mg2+䛿⏕య䛻ᚲせ୙ྍḞ䛺䜲䜸䞁䛷䛒䜛䛜䚸䛭䛾⣽⬊ෆືែ䛻䛴䛔䛶䛿୙᫂䛾䛣䛸䛜ከ䛔䚹䛭䛾୍ᅉ䛜䚸 ඃ䜜䛯 Mg2+ྍど໬ゎᯒ䝒䞊䝹䛾୙㊊䛻䛒䜛䚹≉䛻䚸ỗ⏝䛥䜜䛶䛔䜛ᕷ㈍ Mg2+䝥䝻䞊䝤䛿పศᏊ䛷䛒䜚䚸㛗᫬ 㛫 ᐃ䛻䛚䛔䛶⣽⬊እ₃ฟ䛜ၥ㢟䛸䛺䜛䚹䜎䛯䚸䝥䝻䞊䝤ศᏊ䛾⣲᪩䛔ᣑᩓ䛻䜘䜚䚸⣽⬊ෆᒁᡤ䛾⃰ᗘኚ໬ 䜢ᤊ䛘䜛䛣䛸䜒㞴䛧䛔䚹⣽⬊ෆ Ca2+䛾◊✲䛷ỗ⏝䛥䜜䜛⺯ග⺮ⓑ㉁䝥䝻䞊䝤䛻䛴䛔䛶䜒䚸⣽⬊ෆ Mg2+䛾◊✲ 䛷䛿ᐇ⏝ẁ㝵䛻䛿⮳䛳䛶䛔䛺䛔䚹ᮏ◊✲䛷䛿䚸⏕⣽⬊ෆ䛷Ⓨ⌧䛥䛫䛯䝍䜾䝍䞁䝟䜽㉁䛻⤖ྜྍ⬟䛺᪂つ Mg2+ 䝥䝻䞊䝤䇾MGH䇿䜢㛤Ⓨ䛧䛯䚹᰾䜔⣽⬊⭷ෆⴥ䛺䛹䛾⣽⬊ෆᒁᡤ䛻䝍䜾䝍䞁䝟䜽㉁䜢Ⓨ⌧䛥䛫䚸䛭䛣䛻 Mg2+䝥 䝻䞊䝤䜢≉␗ⓗ䛻ඹ᭷⤖ྜ䛥䛫䜛䛣䛸䛷䚸≉ᐃ䛾䜸䝹䜺䝛䝷䜔⣽⬊ෆ䝗䝯䜲䞁䛻䛚䛡䜛 Mg2+䛾ྍど໬䛜ྍ⬟䛸 䛺䛳䛯䠄ᅗ 1䠅䚹䝥䝻䞊䝤ᢞ୚ᚋ䛛䜙 24 ᫬㛫䛻䛚䛔䛶䜒⣽⬊ෆ䛛䜙༑ศ䛺⺯ග䛜ほᐹ䛥䜜䚸⣽⬊ෆ Mg2+ືែ䛾 㛗᫬㛫ゎᯒ䜒ྍ⬟䛸䛺䛳䛯䚹 ḟ䛻䚸䜰䝫䝖䞊䝅䝇᫬䛾⣽⬊ෆ Mg2+ືែ䛾䜲䝯䞊䝆䞁䜾䜢ヨ䜏䛯䚹䜰䝫䝖䞊䝅䝇䛿ᩘ᫬㛫䛻䛚䜘䜆⣽⬊ෆ䜲 䝧䞁䝖䛷䛒䜚䚸᪤Ꮡ䝥䝻䞊䝤䛷䛿䝸䜰䝹䝍䜲䝮ゎᯒ䛜୙ྍ ⬟䛷䛒䛳䛯䚹MGH 䜢⏝䛔䛶ゎᯒ䛧䛯⤖ᯝ䚸䜰䝫䝖䞊䝅䝇䛻 䜘䜛⣽⬊⦰ᑠ䛾ᚋ䚸⣽⬊ෆ Mg2+⃰ᗘ䛾ቑ኱䛜ぢ䜙䜜䛯䚹 䛣䛾䛸䛝⣽⬊ෆ ATP ⃰ᗘ䛿኱䛝䛟ῶᑡ䛧䚸⣽⬊ෆ Mg2+⃰ ᗘ䛸 ATP ⃰ᗘ䛾㛵ಀ䛻䛿㈇䛾┦㛵䛜ぢ䜙䜜䛯䚹䛩䛺䜟䛱䚸 䜰䝫䝖䞊䝅䝇䛻䛚䛡䜛⣽⬊ෆ Mg2+⃰ᗘୖ᪼䛾※䛜 ATP 䛻㓄఩䛧䛶䛔䛯 Mg2+䛾ゎ㞳䛷䛒䜛䛣䛸䛜ᙉ䛟♧၀䛥䜜䛯䚹 ᮏ⤖ᯝ䛿䚸⣽⬊ෆ Mg2+䛾⃰ᗘไᚚ䛜䝖䝷䞁䝇䝫䞊䝍䞊ά ᛶ䛻䜘䜛䜒䛾䛰䛡䛷䛿䛺䛔䛣䛸䜢♧၀䛩䜛᪂䛯䛺▱ぢ䛷䛒 䜛䚹. ᅗ 1㻌 Mg2+䝥䝻䞊䝤䛾⣽⬊ෆᒁᡤ䜈䛾ᒁᅾ໬.

(15) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌ 研究活動報告. 11. 2䠊 ⤖᰾⳦䛾᪂ᆺ䝦䝮ศゎ㓝⣲ IsdG 䛻㛵䛩䜛◊✲ 䝦䝮ศゎ㓝⣲䛿㔜せ䛺⏕⌮ᶵ⬟䜢ᢸ䛖䛰䛡䛷䛺䛟䚸䝦䝮䛾⮬ᕫ㓟໬䜢ಁ䛩≉Ṧ䛺཯ᛂᶵᵓ䛻䜒ὀ┠䛥䜜䛶 䛔䜛䚹䛭䛾ヲ⣽䛿㏻ᖖᆺ㓝⣲䠄䝦䝮䜸䜻䝅䝀䝘䞊䝊䚸HO䠅䛻䛴䛔䛶䛾䜏䚸䜋䜌ゎ᫂䛥䜜䛶䛝䛯䛜䚸㏆ᖺ䚸HO 䛸 䛿␗䛺䜛䝍䜲䝥䛾䝦䝮ศゎ㓝⣲䛜⑓ཎᛶ⣽⳦䛛䜙┦ḟ䛔䛷Ⓨぢ䛥䜜䛯䚹୰䛷䜒䚸IsdG ᆺ㓝⣲䛿䝦䝮䜢␗ᖖ䛻 ṍ䜑䛶⤖ྜ䛩䜛≉ᚩⓗ䛺ᵓ㐀䜢ᣢ䛱䚸CO 䜢Ⓨ⏕䛧䛺䛔≉Ṧ䛺ᶵᵓ䛷཯ᛂ䜢ゐ፹䛩䜛䠄ᅗ 2䠅䚹๓ᖺ䜎䛷䛻䚸⤖ ᰾⳦䛾 IsdG ᆺ㓝⣲䠄MhuD䠅䛾཯ᛂゎ᫂䛻ᡂຌ䛧䚸䇿Ỉ㓟໬䝦䝮䇿୰㛫య䛾䠎ཎᏊ㓟⣲ῧຍ䛻䜘䜛≉Ṧ䛺㛤⎔ ᶵᵓ䜢᫂䜙䛛䛻䛧䛯䚹ᮏᖺ䛿୺䛻 IsdG ᆺ㓝⣲䛾௦⾲䛷䛒䜛㯤Ⰽ䝤䝗䜴⌫⳦䛾 IsdG 䛻䛴䛔䛶䚸䛭䛾཯ᛂゎ᫂ 䛻ྲྀ䜚⤌䜣䛰䚹 IsdG 䛾䝦䝮௦ㅰ⏘≀䛿 HCHO 䛸䝇 䝍䝣䜱䝻䝡䝸䞁䠄SB䠅䛸ሗ࿌䛥䜜䛶䛔䛯䛜䚸 䜘䜚⏕యෆ䛻㏆䛔཯ᛂ᮲௳䛷䛿␗䛺 䜛⏕ᡂ≀䛜ᚓ䜙䜜䛯䚹ESI-MS 䛸 NMR 䛻䜘䜛ᵓ㐀Ỵᐃ䛾⤖ᯝ䚸䛣䛾⏕ᡂ≀䛿 HCHO 䛜㐟㞳䛧䛶䛔䛺䛔"formyl SB" 䛸Ỵᐃ䛥䜜䛯䠄ᅗ 2䠅䚹formyl SB 䛾⏕ ᡂ䛿ู䜾䝹䞊䝥䛛䜙䜒♧၀䛥䜜䛶䛔䛯 䛜䚸᪤ሗ䛾཯ᛂ᮲௳䛷䛾୺⏕ᡂ≀䛿. ᅗ 2㻌 䝦䝮ศゎ㓝⣲䛾཯ᛂᶵᵓ. formyl SB 䛻㑏ඖ๣䠄䜰䝇䝁䝹䝡䞁㓟䠅䛜௜ຍ䛧䛯䜒䛾䛷䛒䛳䛯䚹ḟ䛻䚸໬Ꮫྜᡂ䛧䛯Ỉ㓟໬䝦䝮䛸 IsdG 䛾」ྜ య䜢ㄪ〇䛧䚸䛭䛾཯ᛂ䜢ゎᯒ䛧䛯䚹䛭䛾⤖ᯝ䚸Ỉ㓟໬䝦䝮䛾㛤⎔䛿 O2 䛾ῧຍ䛾䜏䛷㐍⾜䛧䚸ゐ፹཯ᛂ䛸ྠ୍ 䛾⏕ᡂ≀䛜ᚓ䜙䜜䛯䚹䜘䛳䛶䚸IsdG 䛷䜒䚸௚䛾䝦䝮ศゎ㓝⣲䛸ྠᵝ䚸Ỉ㓟໬䝦䝮䛜୰㛫య䛷䛒䜛䛸⪃䛘䜙䜜䚸䠎 ཎᏊ㓟⣲ῧຍ䛻䜘䜛㛤⎔ᶵᵓ䛜♧၀䛥䜜䜛䚹䛣䜜䜙䛾ᡂᯝ䛛䜙䚸IsdG 䛾୺せ䛺཯ᛂ⤒㊰䛿 MhuD 䛸ྠ➼䛸䜏 䛺䛩䛣䛸䛜䛷䛝䚸௒ᚋ䚸ྠ఩య䝷䝧䝹䛻䜘䜛ᶵᵓ䛾☜ㄆ䜔 HCHO 䛾㐟㞳ᶵᵓ䜒᳨ウ䛧䚸␗ᖖ䛻ṍ䜣䛰䝦䝮䛾 ≉Ṧ䛺ศゎᶵᵓ䛾ゎ᫂䜢┠ᣦ䛩䚹. 3䠊 䝦䝮ศゎ㓝⣲䜢཯ᛂሙ䛸䛧䛶฼⏝䛧䛯ேᕤ㓝⣲䛾๰ฟ 䝦䝮ศゎ㓝⣲䛿ᖹ㠃≧䛾㔠ᒓ㘒య䜢ᐜ᫆䛻ྲྀ䜚㎸䜐䛣䛸䛜䛷䛝䚸୰ᚰ㔠ᒓୖ䛷䛾ᑠศᏊάᛶ໬䛻㐺䛧䛯 άᛶ୰ᚰᵓ㐀䜢᭷䛧䛶䛔䜛䚹䛭䛣䛷䚸䝦䝮ศゎ㓝⣲䜢཯ᛂሙ䛸 䛧䛶⏝䛔䚸ྜᡂ䛧䛯㔠ᒓ㘒య䛸䛾」ྜయ䜢ㄪ〇䛩䜛䛣䛸䛷䚸᪂ 䛯䛺ேᕤ㓝⣲䛾๰ฟ䜢ヨ䜏䛯䚹 䝦䝮ศゎ㓝⣲䛸䛧䛶䝷䝑䝖 HO-1䚸䝆䝣䝔䝸䜰⳦ HmuO 䜢䚸㘒 య䛸䛧䛶Ỉ⣲Ⓨ⏕άᛶ䜢᭷䛩䜛 Co 㘒య䠄ᅗ䠏䚸1, 2䠅䜢⏝䛔䛯䛸 䛣䜝䚸」ྜ໬䛻䜘䛳䛶᭱኱䠔ಸ䛾άᛶྥୖ䛜ぢ䜙䜜䛯䚹ྠ䛨㘒 య䛸䝭䜸䜾䝻䝡䞁䠄௦⾲ⓗ䛺䝦䝮䝍䞁䝟䜽㉁䠅䛾」ྜయ䛷䛿䠎ಸ ⛬ᗘ䛾άᛶྥୖ䛧䛛ㄆ䜑䜙䜜䛪䚸䝦䝮ศゎ㓝⣲䛾᭷⏝ᛶ䛿᫂ 䜙䛛䛷䛒䜛䚹ศග ᐃ䜔䝅䝭䝳䝺䞊䝅䝵䞁䛾⤖ᯝ䚸Co 㘒య䛾⤖ ྜᵝᘧ䛿ᑡ䛺䛟䛸䜒䠎✀㢮䛒䜛䛸⪃䛘䜙䜜䚸䛭䛾⢭ᐦไᚚ䛻䜘䜛 ᭦䛺䜛άᛶྥୖ䜒ᮇᚅ䛥䜜䜛䚹. ᅗ 3㻌 Co 㘒య䛸䝦䝮ศゎ㓝⣲䛾ᵓ㐀.

(16) ◊㻌 ✲㻌 ά㻌 ື㻌 ሗ㻌 ࿌ 研究活動報告. ࠙◊✲άືሗ࿌ࠚ ᩍ ຓ ຓ. ⏕࿨ศᏊࢲ࢖ࢼ࣑ࢡࢫ◊✲ศ㔝䠄2016.1ࠥ2016.12䠅㻌 ᤵ : 㧗ᶫ ⪽ ᩍ : 㙊ᙧ Ύே ᩍ : ᑠ஭ᕝ ᾈஅ. ◊ ✲ ᨭ ᥼ ဨ : 㛫㔝 ⤮᲍Ꮚ ᢏ ⾡ ⫋ ဨ : ୖᯘ ࡉ࠾ࡾ, 㧗ᶫ Ὀே ኱ Ꮫ 㝔 ⏕ : ྜྷ⏣ ᩥ, 㰺⸨ 㞞ᔞ, ᮧ⏣ ᓫே, ఀ⸨ ඃᚿ, ΎỈ ᝆ⏕, ᯇ⏣ ᬛ⣪, ᚚᏊᰘ ┤⣖, Dwiky Rendra Graha Subekti, ஭ᓮ Ᏻ⣪⨾, 㔠ἑ ┬, 㧗ᶫ ᕦ Ꮫ 㒊 ⏕ : Kamonsprasetsuk Supawich, Revyan Magistra Yudhistira 㻌 ᮏ◊✲ศ㔝䛷䛿䚸➨୍䛻㧗᫬㛫ศゎ⬟䛾୍ศᏊ⺯ගほᐹ⿦⨨䜢⏝䛔䛶䚸䝍䞁䝟䜽㉁䛾䝣䜷䞊䝹䝕䜱䞁䜾㐠 ື䜢ほᐹ䛩䜛◊✲䜢ᐇ᪋䛧䛯䚹➨஧䛻䚸⺯ග㢧ᚤ㙾䜢⏝䛔䜛䛣䛸䛷䚸Ⓨ䜺䞁ไᚚ䝍䞁䝟䜽㉁䛷䛒䜛 p53 䛾ᶵ⬟ ゎᯒ䜢⾜䛳䛯䚹➨୕䛻䚸⺯ගほᐹ䛻䜘䜛ศᏊ㑅ู⿦⨨䜢㛤Ⓨ䛧䚸䝣䜯䞊䝆䜢୍ಶయ䛤䛸䛻㑅ู䛩䜛䛣䛸䛷䝍䞁 䝟䜽㉁䛾䝕䝄䜲䞁䜢⾜䛖䛯䜑䛾◊✲䜢⥅⥆䛧䛯䚹 㻝㻚㻌 㧗᫬㛫ศゎ⬟୍ศᏊ⺯ගศගἲ䛻䜘䜛䝍䞁䝟䜽㉁䛾䝣䜷䞊䝹䝕䜱䞁䜾㐠ື䛾ほᐹ㻌 ᙜ◊✲ᐊ䛷䛿䚸䛣䜜䜎䛷䛻ᥦ᱌䛥䜜䛶䛔䜛୍ศᏊ⺯ගほᐹἲ䛾᫬㛫ศゎ⬟䠄1 䝭䝸⛊⛬ᗘ䠅䛻ẚ䜉䛶䚸୍᱆ ௨ୖ㧗䛔᫬㛫ศゎ⬟䜢ᣢ䛴䝷䜲䞁ᆺඹ↔Ⅼ㢧ᚤ㙾䜢㛤Ⓨ䛧䛶䛝䛯䚹ᮏ◊✲䛷䛿䚸䛣䛾⿦⨨䜢⏝䛔䛶䚸䝴䝡䜻 䝏䞁䛾ኚᛶ≧ែ䛻䛚䛡䜛ᵓ㐀䛾୙ᆒ୍ᛶ䛸㐜䛔䝎䜲䝘䝭䜽䝇䜢᫂䜙䛛䛻䛧䛯䚹◊✲䛾ึᮇ䛻䛚䛔䛶䚸 ᐃ䛻⏝ 䛔䜛ὶ㊰⾲㠃䜈䛾ヨᩱ䛾྾╔䛜୍ศᏊ ᐃ䛾෌⌧ᛶ䜢పୗ䛥䛫䜛䛣䛸䜢ぢ䛔䛰䛧䛯䚹䛭䛾䛯䜑䛻䚸྾╔㜵Ṇ 䛾䛯䜑䛾䛥䜎䛦䜎䛺᪉⟇䜢ヨ䛧䛯䚹䛭䛾⤖ᯝ䚸⏕య㐺ྜᛶ䝫䝸䝬䞊䛸䛧䛶▱䜙䜜䜛 MPC 䝫䝸䝬䞊䜢䝉䝹⾲㠃䛻 ඹ᭷⤖ྜ䛥䛫䛶ぶỈ໬䛩䜛䛣䛸䛻䜘䜚䚸ヨᩱ䛾྾╔䛻ཎᅉ䛩䜛ၥ㢟䜢ゎỴ䛧䛯䚹ᚓ䜙䜜䛯䝕䞊䝍䛻䜘䜛䛸䚸ኚᛶ ≧ែ䛾䝴䝡䜻䝏䞁䛻䛚䛡䜛 FRET ຠ⋡䛾ศᕸᖜ䛜ᗈ䛟䚸ኚᛶ≧ែ䛾䝴䝡䜻䝏䞁䛾ᵓ㐀䛾୙ᆒ୍ᛶ䛜♧၀䛥䜜 䛯䚹䛥䜙䛻䚸ኚᛶ≧ែ䛻䛚䛡䜛ಶ䚻䛾 FRET ຠ⋡䛾᫬⣔ิ䝕䞊䝍䜢ㄪ䜉䜛䛸䚸100 µs 䛛䜙 1 ms 䛾㛫䛾᫬㛫㡿 ᇦ䛷䛿᫂ⓑ䛺 FRET ຠ⋡䛾ኚ໬䜢♧䛥䛺䛔䛣䛸䛜ほᐹ䛥䜜䛯䚹䛣䛾䛣䛸䛿䚸ኚᛶ≧ែ䛾䝴䝡䜻䝏䞁䛻䛚䛔䛶䚸䝭 䝸⛊௨ୖ䛾䜖䛳䛟䜚䛧䛯㐠ື䛜Ꮡᅾ䛩䜛䛣䛸䜢♧䛧䛯䚹ᮏ◊✲䛻䛚䛔䛶䚸䝴䝡䜻䝏䞁䛾Ⓨ⌧䜔⢭〇䛿ྎ‴䜰䜹 䝕䝭䜰䝅䝙䜹䛾 Chen ᩍᤵ䜙䛸䛾ඹྠ◊✲䛸䛧䛶ᐇ᪋䛧䛯䚹MPC 䝫䝸䝬䞊䝁䞊䝔䜱䞁䜾䛿䚸ᮾி኱Ꮫ䛾㧗஭ᩍᤵ 䜙䛸䛾ඹྠ◊✲䛸䛧䛶ᐇ᪋䛧䛯䚹ᮏ◊✲䛾ᡂᯝ䜢Ḣᩥᑓ㛛ㄅ䛻Ⓨ⾲䛧䛯䠄M. Saito, S. Kamonprasertsuk, S. Suzuki, K. Nanatani, H. Oikawa, K. Kushiro, M. Takai, P.-T. Chen, E. H.-L. Chen, R. P.-Y. Chen, and S. Takahashi, J. Phys. Chem. B 120, 8818-8829, (2016).䠅䚹 ศᏊ䝅䝱䝨䝻䞁 GroEL 䛜ᇶ㉁䝍䞁䝟䜽㉁䛾䝣䜷䞊䝹䝕䜱䞁䜾䜢᥼ຓ䛩䜛ᶵᵓ䜢䚸୍ศᏊ⺯ගศගἲ䛻䜘䜚ㄪ 䜉䛯䚹㟷Ⰽ⺯ග䝍䞁䝟䜽㉁䠄BFP䠅䜢ᇶ㉁䝍䞁䝟䜽㉁䛸䛧䛶䚸GroEL 䛾ྛ཯ᛂ䝃䜲䜽䝹䛻䛚䛡䜛 BFP 䛾ᵓ㐀ኚ໬ 䜢ほᐹ䛧䛯䚹䛭䛾⤖ᯝ䚸T ᆺ䛾 GroEL 䛻⤖ྜ䛧䛯 BFP 䛾ᵓ㐀䛜୙ᆒ୍䛷䛒䜛䛾䛻ᑐ䛧䚸R ᆺ䛾 GroEL 䛻⤖ྜ 䛧䛯 BFP 䛿䚸཰⦰䛧䛶䜘䜚ᆒ୍䛺ᵓ㐀䛻㌿⛣䛧䛯䚹௚䛾ᇶ㉁䝍䞁䝟䜽㉁䛻䛴䛔䛶ᚓ䜙䜜䛯⤖ᯝ䛸ẚ䜉䜛䛣䛸䛷䚸 GroEL 䛾స⏝䛜ᇶ㉁䛾኱䛝䛥䛻ᛂ䛨䛶␗䛺䜛䛣䛸䛜♧၀䛥䜜䛯䚹. 12.

Fig. 10 䛻 LiG4FSA䚸LiG4TFSA 䜢ྵ䜐␲ఝᅛయ㟁ゎ㉁䜢౑⏝䛧 䛯඲ᅛయ䝸䝏䜴䝮㟁ụ䛾 0.2–10  C 䛷䛾ᨺ㟁ᐜ㔞䜢♧䛧䛯䚹0.2  C 䛾 ᨺ㟁ᐜ㔞䛿 FSA ⣔䛷 155  mAh  g -1 䚸TFSA ⣔䛷 162  mAh  g -1 䛸䚸㧗 䛔ṇᴟ฼⏝⋡䜢♧䛧䛯䚹䛣䜜䛿䚸ṇᴟά≀㉁䛸␲ఝᅛయ㟁ゎ㉁䛾
Fig. 4. 䠍 H-MRI 䛻䜘䜛 Nd 3+ ᢳฟ㐣⛬䛾ྍど໬

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