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(1)Sci.. Repts.. Yokohama. Nail,. Electron. Univ.,. Sec.. II. No.. ,. 119,. October,. Observation. Microscopic. Apparatus. 40, pp,. of HeloTuOPSis. 1993. Egg. of. (Liliaceae). orientalis By. Hiroyuki. ANDOl). May. (Received,. Abstract.. The. tion. the. are. grains. cytoplasm. the. the. the. than Golgi. shaped have. Our. embryo-sac has. not. but. yet. also. come. examined. in. out. flowers. out. in autumn, daytime. by are. in. again. early in. from. a. year.. growth. seasonal. have. become. We. have. intended. developing. and. the. subcellular. structure. 1). Department. of. 2). To. organized. Tokiwadai,日odogaya-ku, all correspondence. to. Yokohama, should. prlOr. Education,. of. 240, be. of H.. Japan. addressed.. the. tO. come. by. one. or. time. orientalis. Our. out. hours. and. state. fertilization. Yokohama. whose. flowers. of two. and. structure. subcellular. orientalis.. orientalis. individuals. temperature. ovules. of H.. microscope, H.. of. whose. natural. investigate. apparatus. Faculty_. their. of. orientalis. electron. the. create. where. obtain. embryo-sac. of egg. Biology,. to. an. state,flowers. chamber in. structure. Heloniopsis. Only. could. to. seem. microscopic. individuals. changes. we. the. whom. a. not. richer. a. in complicated horseshoeare. synergids. scanty.. natural we. another. and in. Furthermore,. have. there The. very. uslng the. But. spring.. summer. breeding. is,. yet. egg. more. electron. and been. embryologically,. shifted That. seasons.. and. subcellular. microscope.. very. come. are organelles Characteristically,. metabolism.. In. The. their. with also in cell has a. cells and. in its cytoplasm, Both the synergids. physiology. has. plastids丘11ed. three tip.. nucellar. in structure.. Angiosperms. light. a. cells at the few organelles. cytoplasm.. the. on. of these. organelles. in the synergid. in. activity. knowledge in. egg. Large. epidermis.. cytoplasm. cell and. apparatus. high. a. the. are. egg. the. nucellar. epidermal. immature. are. organelles than. a. in. there. and. cytoplasm structure. with. contained. of. cytoplasm. poor. apparatus. is overlaid. synergids starch. egg. 10, 1993). to fertilizaapparatus prior of egg investigated the transmission using electron two is composed egg which of one cell and. was. on'entalis. The. microscope.. SAT61)2). structure. subcellular. in Heloniopsis. Yoshihiko. and. paper. presents. in this. National. of. species.. University,.

(2) H.. 2. ANDO. Material Many. individuals. Yamanashi. Prefecture. have■. them. beeli. University,. planted some. and. mentioned.. The. flower. hid. which. buffered ide. 0.1 M. in. graded. of. JEM-100CX. several The. Leblond. The. fixed. viscosity. uranyl. acetate were. of. an. of. tetrox-. osmium. in. (Spurr. resin. lead by. stained. ovary. dehydrated. epoxy. and. above. glutaraldehyde. l%. were. a. 1969). For. citrate.. silver. were. sections. Many. National. from. with. materials. in low. in. chamber. in 2.5%. post-fixed. stained. electron. growth. excised. were. sections. 1967).. the. were. Kiyosato. Yokohama. of the in. at. Prefecture.. lbaraki. bred. and. 2%. with some. transmission. campus. These丘Ⅹed. embedded. stained. the. clusters. buffer. and. in. ovules. buffer. phosphate. carbohydrate, &. (Rambourg. M. were. of. collected. were. City. been. have. out.. series. sections. detection. come. phosphate. ethanol. Many. clusters. 0.1. with. them. of. on. grown. and. Method. orientalis. Hitachi. at. and. SAT6. and. Heloniopsis. of. Y.. and. the. methanamine the. under. examined. microscope.. Observation l.. of. Nu(:ellus. Heloniopsis. orientali･s has. the. is. ovules. Several. the. 2⊥3. some. ization.. Several. contained. in. the. they. smaller. are. (a) An and. stained Egg Egg egg two. of. cells at the are. those these. in. present the. plastids. silver. in the. to. epidermal. be. at. starch. the of. become. to. fertil-. after. (Fig.. an. of. fertiliza-. 1A). are. is. embryo･sac in. infrequent. quite. cells. epidermal seems. or. tip. Before. grains. where. material. micropylar. persists. large. of nucellus. and The. the. periclinally. epidermis. (Fig. 1B). absent are. tip. The. into. material.. several. at. epidermis embryo-sac.. change. divide. usually. with. If they. with. tip. nucellar. the. with. plastids丘11ed. they. than. overlaid. an. nucellus. integuments.. two. of developing to. A. ovary.. with. develops.. embryo-sac. multi-layered. epidermal. in. the. as. an. between. destroyed. partially. large. nucellus.. contained. 2.. cell. of. are. grains. the. the. and. However,. flank. they. cells. end. gradually. is thinly. embryo-sac. cel･1ed layers,. micropylar. are. density,. epidermal. organized.. the. and. intervene. nucellus. in. ovules. is invested. it. and. the. of. cells. electron. organized. tion.. tip. nucellar. high. with. cells. nucellar. intervening. anatropous. crassinucellate. vegetative. the. near. many. cells. ones. the. at. tip.. nucellar. at. flank, The since. carbohydrate,. methanamine.. apparatus. cell ､apparatus. synergids.. of. the The. organized egg. cell. is. embryo-sac. (Fig. 2A). is. constructed. enclosed. with. of a. one. thin. egg. but.

(3) Egg. Apparatus. of Heloniopsis. on'entalis.

(4) H.. ANDO. and. Y.. SAT6.

(5) Egg. Apparatus. of. Heloniopsis. orientalis. 惑. 惑璽窪整 %. 望寵蛋藍援護濃.

(6) H.. ANDO. and. Y.. SAT6.

(7) Apparatus. Egg. irregular. iロ in. plastids the. Shape. the. well. are. In. is. plastid. matrix. the. matrix. of. mitochondrion.. are. which. evenly. plasm which. the. like. cisternae. Golgi. apparatus and. cally 丘ne. Each. of. osmiophilic like. shaped. Ends. few. are. Golgi. of. vesicles. or. plastids. the. within. the. the. (Fig. 3B). all of. the. of. the. characteristi-. of the. Very. Golgistack. apparatus. of the. periphery. the. from. (Fig. 4A).. face. Golgi. of. ip. stacked. different. pro丘1e. trams. Golgi. in. 4-6. synergids. sectional. cyto-. synergid lump. Golgi丘elds. But. cell.. cisterna at. 4B). It appears. composed. egg. with. each. form. is. in. invested. the. grain. These. of synergid. to. the. a. Nearly. threads.. of them. are. than. starch. the in. thickly. assemble. horseshoe. a. vesicles. horseshoe.. a. there. although. into. con-. (Figs. 4A,. plastids. throughout. apparatus. cell, many. bend. strongly. and. egg. mem-. are. density. a. of. inner. globules. (Fig. 4C).. half. within. Many. cytoplasm.. micropylar. apparatus. develop. cristae. Their. 丘11ed with. tangled. Golgi. throughout. matrix.. lipid. the. gathers. apparatus. their. electron. of. synergid. as. the. at. in. methanamine. partially. proBle. Golgi. several. cytoplasm.. synergid. Golgi. of. distributed. that. sections. higher. permeates. ER. in. invariably. the. their. several. and. exceed. distributed. mitochondria.. some. silver. throughout. in sectional. are. the. is. are. with. stained. cisterna. appears. apparatus. they. meaningfully. evenly. contained. immature which. 3B). of mitochondria,. than. numerou占ribosomes the. and. are. very. and. scattered with. (Fig.. Furthermore,. large. greatly. ER. globules larger. however,. in. are. all. 7. orientalis. almost. nearly. lipid. tained. grains. are. considerably. system,. become. Mitochondria. few. a. and. plastids. brane. Mitochondria. cytoplasm.. synergid. rather. large.. and. number.. of Heloniopsis. swell. out,. apparatus.. Discussion. (1) Nucellus The. micropylar. epidermis. of. a. with. are. (Sat6. epidermal. and. cell. or. tube. pollen that. cells. the. micropyle. orientalis. embryoISaC. epidermis. The. 1976a) some. the. tip. of. at. the. tip. of. enters. middle dissolve examined. into. the. nucellus. the. lamellae. nucellus.. of. before. the. by. the. us,. the. change. pollen change. in. tube. of epidemal. whose. in. Aucuba. is overlaid. and Wilms. between at. the. cell. or. from. the. pollen. epidermal. this. (1981a,. part. the. b) observed. embryo-sac. nucellar. an. with the. the. micropyle,. shape. arrives. plants. Just ￣before. the. tissue. overlaid. embryo-sac. 1976b). the. with. embryo-sac. nucellus.. I,n Spinacia,. nucellar. of. (Sate. of. overlaid. anglOSpermOuS. through. nucellus. An. tip. praecox layers. subepidermal. at. the. micropylar. Stachyurus. and. for. is. orientalis. fertilization.. known. is well. H.. Of. after. and. crassinucellate.. japonica. arrives. of. before. nucellus. nucellar. nucellus. tube. end. tip.. cells in shape. and In. H.. or/and.

(8) dissolution. the. of But. served.. more. and. in. of nucellus. the. cell at. metabolic. and. (2). Egg. The. low. apparatus. with. the. That. is, both. physiology. egg. orientalis. the. that. the. at. are. grain. starch. seems. ob-. the. epidermal. in physiological. some. relation. to. organelles. and. of. bear. in other. well-developed. to are. There. the There. few. the. before. same. the. entry. of. of. egg. egg. a. of the. Went. of. egg. H.. and. compared structure.. high. 1984),. 1965). apparatus. ribosomes. in. apparatus has. been the. although. mitochondria is. in. activity. which. many. very. cytoplasm,. synergids,. have. has. (Jensen. ribosomes the. cell. synergid. apparatus. &. cell is. egg. subcellular. to. (Willemse. the. where. that. in. of them. each. with. the. structure. structure. plants. many. both. seem. subcellular. angiospermous. with. ER. complicated. as. Gossypium. mitochondria,. fertilization. The. the. the. since. also. that. seems. Within. actively,. comparatively. the. it. as. such. function. it is noteworthy as. has. activities.. organelles. is nearly. ER. 2.. with. ones. nucellus. the且ank. Therefore,. metabolism.. of. 1.. It. latter.. to. metabolic. synergids. apparatus. features. and. cell, the. and. However,. Blled. plastids. the at. fertilization. structure.. seem. egg. examined. seems. in. hand,. other. Golgi. H.. This. di庁erent. are. been. not. from. nucellus.. physiological. the. on. ones. before. cell. Immature. in. in. than. have. apparatus. egg. very. the. tip. nucellar. tip exceeds. activity. into. tube. pollen. former. tissue. nucellar. of plastids;. appearance. nucellar. SAT6. the. of. cells at the. in the. larger. Y.. and. lamellae. middle. epidermal. flank. is. ANDO. H.. 8. known. and. as. orientalis. well.. has. the. follows: are. large. of egg. cytoplasm are. with. plastids丘11ed cell. two. and. horseshoe-shaped. Golgi. large. grain. or. grains. of. starch. in. synergids. in. apparatus. the. cytoplasm. of two. synergids. We of. will. embryo-sac. report. after. in. near. future. fertilization. on. and. the so. process on. in. H.. of. fertilization,. the. structure. orientalis.. ショウジョウバカマ(Heloniopsis orientalis,ユリ科)の受精前の卵装置の構造を透 2枚の珠皮が厚層珠心を囲んでいる｡過型電子顕微鏡を用いて観察した｡腫株は倒生で, 珠心表皮は部分的に並層分裂を行い, 2-3細胞層になり,受精後も消えずに生き残っている｡卵装置はこの表皮で覆われていることになる｡大きなデンプン粒で満たされた大形の色素体は,珠心側部の表皮細胞には含まれていても数は少ないが,珠心先端部の表皮細胞には必ず含まれており,数も多い｡卵装置は1個の卵細胞と繊形装置の発達した2 個の助細胞で構成されている｡受精前の2個の助細胞には構造上の相違は特にはみられ.

(9) Egg. of HelonioPsis. Apparatus. 9. orientalis. ない｡卵装置を構成する3細胞にも,デンプン粒を含む大形の色素体が存在する｡卵細胞の大部分は液胞で占められており,核は合点端に位置している｡核の周辺にはいろいろな細胞内小器官がみられるが,数は少なく,構造も単純である｡助細胞では,多数のリボソームを伴った粗面小胞体がよく発達し,もつれた糸が作る糸玉のように塊状に集まった小胞体が部分的にみられる｡クリステの発達したミトコンドリアも量的にかなり多く存在している｡繊形装置付近には多数のゴルジ体がみられるが,強く湾曲し馬蹄形状のゴルジ体が助細胞に特徴的に存在している｡馬蹄形状のゴルジ体とデンプン粒を含む大形の色素体の存在が,ショウジョウバカマの卵装置の構造的な特徴と思われる｡ Referenees A.. W.. JENSEN,. 1965 Amer.. cotton.. A.. RAMBOロRG,. Y.. sAT6,. T6hoku sptJRR, J.. M. M.. H. Neerl.. WILMS,. H.. Neerl.. Ser.. 1969. Ultrast.. B. WILMS,. Embryological. R.. WILLEMSE,. the. studies. T.. M.. studies. central. and. basement. of. 20:. cell of. and. membrane. periodic. and. acid-silver. 306-309.. cornaceous. some. on. Stachyurus. embedding. S°i. Rep.. plants.. T6hoku. PraeCOX. its. and. for. medium. Sci･. variety･. electron. Rep･. microscopy･. 31-34. WENT,. J.. L.. van. 1984. of Angiosperms.. (ed.), Embryology. ∫. 1981a. of. low-viscosity. and. egg. 117-130.. 131-138.. 26:. the. acid-Sciff. Res.. ⅠⅤ (Biol.), 37:. A. Res.. Staining. periodic. ∫. Ultrast.. ⅠⅤ (Biol.), 37:. Univ.. A.. by. Embryological. 1976b. of. composition. 1967. LEBLOND. techniques.. Ser.. and. 52: 78ト797. P.. structures. 1976a. Y.. C.. and. methanamine. Univ.. ultrastructure. ∫. Bot.. M.. associated. sAT6,. The. Ultrastructure. of the. pp.. developing. The. female. 159-196. embryo. gametophyte.. In. Springer-Verlag, sac. of spinach.. JoI寸RI, Berlin･. Acta. Bot･. Acta. Bot･. 30: 75-99,. ∫. 1981b 30:. Pollen. 101-122.. tube. penetration. and. fertilization. in. spinach..

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