スライド 1

県立広島病院 輸血部

藤井 明美

a b c ｄ

1 25D04 ^{Ｈ-1,Ｈ-2 ○}

2 25D06 ^{○ Ｌ-1 ○ ○ ○}

3 25D10 ^{○ Ｌ-4 ○}

4 25D11 ○ Ｌ-2 ○

5 25D12 ○ Ｌ-1 ○

6 25D14 ○ Ｌ-1 ○

7 25D16 ^{H-1(Ｈ2504) メーカー}

8 25D17 ^{○ Ｌ-1 ○}

9 25D18 ^{Ｌ-1 ○}

10 25D19 ^{○ Ｌ-1 ○}

11 25D21 ^{○ Ｌ-1 ○}

12 25D22 ^{○ Ｌ-3 検査センター}

13 25D26 ^{Ｌ-1 ○ ○}

14 25D27 ○ Ｌ-1 ○

15 25D29 Ｌ-2,H-1 ○ ○ ○

16 25D31 Ｌ-1 ○

17 25D35 ^{○ Ｌ-1 ○}

18 25D37 ^{○ Ｌ-1 ○}

19 25D40 ^{○ Ｌ-1 ○ ○}

20 25D42 ^{○ 検査センター}

造血幹移植 その他

使用試薬

No. DNA-QC Lab. No.

参加部門

S S P法のみ 輸血関連 臓器移植

a b c ｄ

造血幹移植 その他

使用試薬

No. DNA-QC Lab. No.

参加部門

S S P法の み 輸血関連 臓器移植

21

25D44

22

25D45

23

25D47

24

25D49

25

25D50

25D51

27

25D52

28

25D53

29

25D56

30

25D59

31

25D60

32

25D62

33

25D63

34

25D67

使用試薬名

•Low resolution

L-1 MocroSSPJPN

L-2 MicroSSPABDR

L-3 MicroSSP1L/2L/1A/1C

L-4 AllsetGold

•High resolution

H-1 MicroSSPAlleleSpecific

H-2 Unitray

SSP 単独 22 施設

Low resolution

ONELAMBDA MicroSSP JPN 18 施設 ONELAMBDA MicroSSP ABDR 1 施設 ONELAMBDA MicroSSP 1L/2L 1 施設 Invitrogen AllsetGold 1 施設

SSP+SSO,SBT 9(5) 施設

Low resolution

ONE LAMBDA MicroSSP JPN 4 施設 ONE LAMBDA MicroSSP 1A/1C/2L 1 施設

High resolution

ONE LAMBDA MicroSSP Allele Specific 3 施設

Invitrogen Unitray 1 施設

 低解像度（low resolution）のタイピング法

HLA 抗原（型）レベルの結果を目的として実施した場合 は第1区域でアリル表記するものとし第2区域でアリル表記 をしない．

＊ 第1区域において判別できない結果の場合は他の試薬kitまたはタイ ピング法により判別することが望ましい．

 中または高解像度（ high resolution ）のタイピング法

第 2 区域までの表記が必要．

 miss assign

 表記 ?

 解析困難

黄色

緑色

桃色

 「 //+ 」使用の表記

 「 * 」 ⇒ 「＊」

 コロン（ : ）未記入

 第一区域での ambiguty 表記

⇒原則として用いない

（ HLA タイピング結果のアリル表記法と結果報告の原則～ 2010 年度版）

No. Lab.ID HLA-A HLA-B HLA-C

1 25D04

A*24:02/21/100/+ 実施なし

B*44:03/60/61/+ 実施なし

C*14:03/10/22 C*15:02/26/28/+

2 25D06 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

3 25D10 A*24:02/04/05/+ A*33:01/02/03/+

B*44:02/03/04/+ B*51:02 4 25D11 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/07/+ B*51:02/34

5 25D12 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

6 25D14 A＊24:02/03/04/+ A＊33：01/03/04+ B＊44:03/07/13/+ B＊51:02/23 C＊14:03/22 C＊15:02/05/06/+

7 25D17 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 Cw*1402/03/05/+ Cw*1502/04/05/+

8 25D18 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

9 25D19 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

10 25D21 A*24:02/03/04/+ A*33:01/03/04/+ B*44:03/07/13/+ B*51:01/03/07/+ C*14:03/22 C*15:02/05/06/+

11 25D22 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/06/+ B*51:02/34 C*14:02/03/04/+ C*15:02/07/08/+

12 25D26 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

13 25D27 A*24:02/03/04/+ A*33:01/02/03/+ B*44:02/05/11/+ B*51:02/23 C*14:02/05/06/+ C*15:02/05/06/+

14 25D29 A*24:02/26/98/+ A*33:03/25/26/+ B*44:03/30/111/+ B*51:02 C*14:03/10/22 C*15:02/08/10/+

15 25D31 A*24:02//+ A*33:01/03/04/+ B*44:02//+ B*51:02/23/42

16 25D35 A*24:02/03/04/+ A*33:01/03/04/+ B*51:01/03/07/+ B*44:55 C*14:02/05/06/+ C*15:02/05/06/+

17 25D37 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/05/11/+ B*51:02/23 C*14:02/05/06/+ C*15:02/05/06/+

18 25D40 A*24:02/03/04/+ A*33:01/02/03/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

19 25D44 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

20 25D47 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02 C*14:02/03/05/+ C*15:02/04/05/+

21 25D49 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

22 25D50 A*24:02/03/04/+ A*33:01/02/03/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

23 25D51 A*24 A*33 B*51 B*44/51 C*14 C*15

24 25D52 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

25 25D53 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ -

26 25D59 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

27 25D62 A*24:02/03/04/+ A*33:01/03/04/+ B*44:02/03/06/+ B*51:02/34

28 25D63 A*24:02/03/04/+ A*33:01/03/04/+ B*44:03/07/13/+ B*51:02/23 C*14:02/05/06/+ C*15:02/05/06/+

29 25D67 A*24:02/03/04/+ A*33:01/03/04/+. B*44:02/03/05/+ B*51:02/23 C*14:02/03/05/+ C*15:02/04/05/+

 25D21

4G false positive

 25D35

4G 6D false positive

 25D27 25D37 25D53

判定間違い（解析ソフト使用なし？）

 表記?

HLA-A33

HLA-A*33:01/02/03/+ ⇒ 33:02欠番 HLA-B44

判定表:HLA-B*44:02/05/11/+とHLA-B*44:03/07/13/+のambiguity 解析ソフト:HLA-B*44:02/03/05/+

HLA-Cw14

判定表：HLA-C*14:02/05/06/+とHLA-C*14:03/22のambiguity

解析ソフト:HLA-B*14:02/03/05/+

No. Lab.ID HLA-A HLA-B HLA-C 1 25D04

A*24:02/21/100/+ 実施なし

B*52:01/07/08/+ 実施なし

C*12:02/22/27/+ 実施なし

2 25D06 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+

3 25D10 A*02:03/25/38/+ A*24:02/04/05/+

B*52:01/04/05/+ B*56:01/16/19/+

4 25D11 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+

5 25D12 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/08/+ C*01:02/03/04/+ C*12:02/03/04/+

6 25D14 A＊02:01/02/03/+ A＊24:02/03/04/+ B＊52:01/05/06/+ B＊56:01/02/08/+ C＊01:02/03/05/+ C＊12:02/03/04/+

7 25D17 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ Cw*01:02/03/04/+ Cw*12:02/03/04/+

8 25D18 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+

9 25D19 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+

10 25D21 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/08/+ C*01:02/03/05/+ C*12:02/03/04/+

11 25D22 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/07/+ C*12:02/14/16/+

12 25D26 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+

13 25D27 A*02:01/02/03/+ A*24:02/03/04/+ B52:01/05/06/+ B*56:01/02/08/+ C*01:02/03/05/+ C*12:02/03/04/+

14 25D29 A*02:01/02/03/+ A*24:02/21/98/+ B*52:01/05/06/+ B*56:01

15 25D31 A*02:01//+ A*24:02//+ B*52:01//+ B*56:01/02/04/+

16 25D35 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/08/+ C*01:02/03/05/+ C*12:02/03/04/+

17 25D37 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/08/+ C*01:02/03/05/+ C*12:02/03/04/+

18 25D40 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+

19 25D44 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+

20 25D47 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/08/+ C*01:02/03/04/+ C*12:02/03/04/+

21 25D49 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/05/+ C*12:02/03/04/+

22 25D50 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+

23 25D51 A*02 A*24 B*49/52 B*56 C*01 C*12

24 25D52 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+

25 25D53 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+

26 25D59 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*12:02/03/04/+

27 25D62 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+

28 25D63 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/08/+ C*01:02/03/05/+ C*12:02/03/04/+

29 25D67 A*02:01/02/03/+ A*24:02/03/04/+ B*52:01/05/06/+ B*56:01/02/04/+ C*01:02/03/04/+ C*06:02/04/06/+

 25D67

8B false positive

 表記 ? HLA-B56

判定表:HLA-B*56:01/02/08/+とHLA-B*56:04/27 のambiguity

解析ソフト :HLA-B*56:01/02/04/+

No. Lab.ID HLA-A HLA-B HLA-C 1 25D04

A*03:01/04/17/+ 実施なし

B*07:02/52/55/+ 実施なし

C*07:02/56/75/+ 実施なし

2 25D06 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/03/05/+ C*07:01/02/03/+

3 25D10 A*03:01/03/04/+ A*24:02/04/05/+

B*07:02/05/06/+ B*44:02/03/04/+

4 25D11 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/03/07/+

5 25D12 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/03/05/+ C*07:01/02/03/+.

6 25D14 A＊03:01/02/03/+ A＊24:02/03/04/+ B＊07:02/07/09/+ B＊44:02/05/11/+ C＊05:01/03/05/+ C＊07:01/02/03/+

7 25D17 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ Cw*05:01/03/05/+ Cw*07:01/02/03/+

8 25D18 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/03/05/+ C*07:01/02/03/+

9 25D19 A*03:01/02/03/+ A*24:02/03/04/+ B*07:05/06/80/+ B*44:03/07/13/+ C*05:01/03/05/+ C*07:01/02/03/+

10 25D21 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:03/07/13/+ C*05:01/03/05/+ C*07:01/02/03/+

11 25D22 A*03:01/02/04/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/03/07/+ C*05:01/03/04/+ C*07:01/02/05/+

12 25D26 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/03/05/+ C*07:01/02/03/+

13 25D27 A*03:01/02/03/+ A*24:02/03/04/+ B07:05/06/80/+ B*44:02/05/11/+ C*05:01/02/03/+ C*07:01/02/03/+

14 25D29 A*03:01/04/83/+ A*24:02/98/101/+ B*07:02/22/58/+ B*44:02/112/113/+ C*05:01/07/08/+ C*07:02/32/56/+

15 25D31 A*03:01//+ A*24:02//+ B*07:02/05/06/+ B*44:02/03/05/+

16 25D35 A*03:01/02/03/+ A*24:02/03/04/+ B*07:05/06/80/+ B*44:03/07/13/+ C*05:01/03/05/+ C*07:01/02/03/+

17 25D37 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:03/07/13/+ C*05:01/03/05/+ C*07:01/02/03/+

18 25D40 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/02/03/+ C*07:01/02/03/+

19 25D44 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/03/05/+ C*07:01/02/03/+

20 25D47 A*03:01/02/04/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/03/05/+ C*07:01/02/03/+

21 25D49 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/02/03/+ C*07:01/02/03/+

22 25D50 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/02/03/+ C*07:01/02/03/+

23 25D51 A*03 A*24/31/33 B*07 B*44 C*05 C*07

24 25D52 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/03/05/+ C*05:01/03/05/+ C*07:01/02/03/+

25 25D53 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/03/05/+ C*07:01/02/03/+

26 25D59 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/03/05/+ C*07:01/02/03/+

27 25D62 A*03:01/02/04/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/03/07/+

28 25D63 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/03/05/+ C*07:01/02/03/+

29 25D67 A*03:01/02/03/+ A*24:02/03/04/+ B*07:02/07/09/+ B*44:02/05/11/+ C*05:01/03/05/+ C*07:01/02/03/+

 25D19 25D35

4C 4F false positive

 25D21 25D37 4F false positive

 25D27

4C false positive

No. Lab.ID HLA-A HLA-B HLA-C

1 25D04 A*31:01/15/16/+ 実施なし

B*39:02 実施なし

2 25D06 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

3 25D10 A*26:01/02/04/+ A*31:01/05/09/+

B*39:02/08/13/+ B*15:01/04/07/+

4 25D11 A*26:01/02/03/+ A*31:01/02/04/+ B*39:02/08/13/+ B*15:01/07/26/+

5 25D12 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+.

6 25D14 A＊26:01/02/03/+ A＊31:01/02/04/+ B＊15:01/04/07/+ B＊39:02/08/13/+ C＊03:03/11/12/+ C＊07:01/02/03/+

7 25D16 B*15:01/50/102/+ B*39:02/08/13/+

8 25D17 A*26:01/02/03/+ A*31:01/02/04/+ B*39:02/08/13/+ B*15:01/04/07/+ Cw*07:01/02/03/+ Cw*03:03/11/12/+

9 25D18 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

10 25D19 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

11 25D21 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

B*15:01/102/125/+ B*39:02/08/13/+

12 25D22 A*26:01/02/04/+ A*31:01/02/04/+ B*39:02/08/13/+ B*15:01/07/27/+ C*03:03/11/13/+ C*07:01/02/05/+

13 25D26 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

14 25D27 A*26:01/02/03/+ A*31:01/02/04/+ B15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

15 25D29 A*26:02 A*31:01/25/27/+ B*15:01/50/102/+ B*39:02 C*03:03/52/53/+ C*07:02/56/75/+

16 25D31 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+

17 25D35 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*07:01/02/03/+ C*03:03/11/12/+

18 25D37 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

19 25D40 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

20 25D44 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

21 25D47 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

22 25D49 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

23 25D50 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

24 25D51 A*26 A*31 B*15 B*39 C*03 C*07

25 25D52 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

26 25D53 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*07:01/02/03/+ C*03:03/11/12/+

27 25D59 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

28 25D62 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/07/27/+ B*39:02/08/13/+

29 25D63 A*26:01/02/04/+ A*31:01/02/04/+ B*39:02/08/13/+ B*15:01/04/07/+ C*07:01/02/03/+ C*03:03/11/12/+

30 25D67 A*26:01/02/03/+ A*31:01/02/04/+ B*15:01/04/07/+ B*39:02/08/13/+ C*03:03/11/12/+ C*07:01/02/03/+

No. Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1

1 25D04 実施なし 実施なし 実施なし 実施なし DQB1*03:03/20/30/+ DQB1*06:04/34/36/+

2 25D06 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01 DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

3 25D10 DRB1*09:01/02/03/+ DRB1*13:01/02/16/+ DRB3*01/02/03 DRB4*01:01/02/03/+

4 25D11 DRB1*09:01/02/03/+ DRB1*13:02/21/23/+ DRB3*01/02/03 DRB4*01:01/02/03/+

5 25D12 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01:01/02:01/+ DRB4*01:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

6 25D14 DRB1＊09:01/02/03/+ DRB1＊13:01/02/03/+ DRB3＊01/02/03 DRB4＊01 DQB1＊03:03/06/12/+ DQB1＊06:01/02/03/+

7 25D17 DRB1*0901/02/03/+ DRB1*1301/02/03/+ DRB3*0101/02/03/+ DRB4*0101/02/03/+ DQB1*0601/02/03/+ DQB1*0303/06/12/+

8 25D18 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

9 25D19 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB4*01:01/02/03/+ DRB3*01:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

10 25D21 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

11 25D22 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

12 25D26 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01//+ DRB4*01 DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

13 25D27 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01:01/02/03/+ DRB4*01:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

14 25D29 DRB1*09:01/11/12/+ DRB1*13:02/99/124/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:03/30/31/+ DQB1*06:04/34/36/+

15 25D31 DRB1*09:01/02/03/+ DRB1*13:01//+ DRB3*01/02/03 DRB4*01

16 25D35 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01 DQB1*06:01/02/03/+ DQB1*03:03/06/12/+

17 25D37 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01 DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

18 25D40 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

19 25D44 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

20 25D47 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01:01/02/03/+ DRB4*01:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

21 25D49 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

22 25D50 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01 DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

23 25D51 DRB1*09 DRB1*13 DRB3*01/02/03 DRB4*01 DQB1*03/04 DQB1*06

24 25D52 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB4*01:01/02/03/+ DRB3*01/02/03 DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

25 25D53 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01:01/02/03/+ DRB4*01:01/02/03/+ DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

26 25D59 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01 DQB1*03:03/06/12/+ DQB1*06:01/02/03/+

27 25D62 DRB1*09:01/02/03/+ DRB1*13:02/21/23/+ DRB3*01/02/03 DRB4*01

28 25D63 DRB1*09:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB4*01 DQB1*06:01/02/03/+ DQB1*03:03/06/12/+

No. Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1

1 25D04 DRB1*13:12/118 実施なし 実施なし 実施なし 実施なし 実施なし

2 25D06 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

3 25D10 DRB1*13:07/12/38/+ DRB1*15:01/02/03/+ DRB5*01/02 DRB3*01/02/03 4 25D11 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02

5 25D12 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01:01/02:01/+ DRB5*01:01/02:02/+ DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

6 25D14 DRB1＊15:01/02/03/+ DRB1＊13:01/02/03/+ DRB5＊01/02 DRB3＊01/02/03 DQB1＊03:01/04/09/+ DQB1＊06:01/02/03/+

7 25D17 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB5*01:01/02/03/+ DRB3*01:01/02/03/+ DQB1*06:01/02/03/+ DQB1*03:01/04/09/+

8 25D18 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

9 25D19 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01:01/02/03/+ DRB5*01:01/02/03/+ DQB1*06:01/02/03/+ DQB1*03:01/04/09/+

10 25D21 DRB1*15:01/02/03/+ DRB1*13:01/02/03/+ DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

11 25D22 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

12 25D26 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01//+ DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

13 25D27 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01:01/02/03/+ DRB5/01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

14 25D29 DRB1*13:12 DRB1*15:02/44/47/+ DRB3*01/02/03 DRB5*01/02 15 25D31 DRB1*13:01//+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02

16 25D35 DRB1*15:01/02/03/+ DRB1*13:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*06:01/02/03/+ DQB1*03:01/04/09/+

17 25D37 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*06:01/02/03/+ DQB1*03:01/04/09/+

18 25D40 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

19 25D44 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

20 25D47 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01:01/02/03/+ DRB5*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

21 25D49 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

22 25D50 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

23 25D51 DRB1*13 DRB1*15 DRB3*01/02/03 DRB5*01/02 DQB1*03 DQB1*06

24 25D52 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

25 25D53 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01:01/02/03/+ DRB5*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

26 25D59 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*03:01/04/09/+ DQB1*06:01/02/03/+

27 25D62 DRB1*13:01/02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02

28 25D63 DRB1*13:01//02/03/+ DRB1*15:01/02/03/+ DRB3*01/02/03 DRB5*01/02 DQB1*06:01/02/03/+ DQB1*03:01/04/09/+

No. Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1

1 25D04 DRB1*01:01/17/18/+ DRB1*04:03/71/93/+ 実施なし 実施なし

DQB1*05:01/07/11 実施なし

2 25D06 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

3 25D10 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ - 4 25D11 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ -

5 25D12 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

6 25D14 DRB1＊01:01/02/04/+ DRB1＊04:01/02/03/+ DRB4＊01 - DQB1＊05:01/02/03/+ DQB1＊03:02/05/07/+

7 25D17 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*05:01/02/03/+ DQB1*03:02/05/07/+

8 25D18 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

9 25D19 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*05:01/02/03/+ DQB1*03:02/05/07/+

10 25D21 DRB1*01:01/02/04/+ DRB1*04:01/03/04/+ DQB1*05:01/02/03/+ DQB1*03:02/05/07/+

11 25D22 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

12 25D26 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

13 25D27 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

14 25D29 DRB1*01:01/38/41/+ DRB1*04:03/93/96/+ DRB4*01:01/02/03/+ - DQB1*03:02/32 DQB1*05:01/07/11/+

15 25D31 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01 -

16 25D35 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01 - DQB1*05:01/02/03/+ DQB1*03:02/05/07/+

17 25D37 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ ― DRB4*01 DQB1*05:01/02/03/+ DQB1*03:02/05/07/+

18 25D40 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

19 25D44 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

20 25D47 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

21 25D49 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

22 25D50 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01 DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

23 25D51 DRB1*01 DRB1*04 DRB4*01 DQB1*03 DQB1*05

24 25D52 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

25 25D53 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01:01/02/03/+ DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

26 25D59 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01 - DQB1*03:02/05/07/+ DQB1*05:01/02/03/+

27 25D62 DRB1*01:01/02/04/+ DRB1*04:01/02/03/+ DRB4*01 -

28 25D63 DRB1*01:01/02/04/+ DRB1*04:01/03/04/+ - DRB4*01 DQB1*05:01/02/03/+ DQB1*03:02/05/07/+

No. Lab.ID HLA-DRB1 HLA-DRB3/4/5 HLA-DQB1

1 25D04 DRB1*14:06/108/121 DRB1*14:54/70/75/+ 実施なし 実施なし 実施なし 実施なし

2 25D06 DRB1*14:01/02/04/+ - DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

3 25D10 DRB1*14:01/14/23/+ DRB1*14:02/06/13/+ DRB3*01/02/03 DRB3*01/02/03

4 25D11 DRB1*14:01/02/03/+ - DRB3*01/02/03 -

5 25D12 DRB1*14:01/02/05/+ - DRB3*01:01/02:01/+ - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

6 25D14 DRB1＊14:01/02/05/+ - DRB3＊01/02/03 - DQB1＊03:01/04/09/+ DQB1＊05:01/02/03/+

7 25D16 DRB1*14:54/82 DRB1*14:06/81/108

8 25D17 DRB1*14:01/02/04/+ - DRB3*01:01/02/03/+ - DQB1*05:01/02/03/+ DQB1*03:01/04/09/+

9 25D18 DRB1*14:01/02/04/+ - DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

10 25D19 DRB1*14:01/02/04/+ - DRB3*01:01/02/03/+ - DQB1*05:01/02/03/+ DQB1*03:01/04/09/+

11 25D21 DRB1*14:01/02/05/+ DRB1*14:01/02/05/+ DQB1*05:01/02/03/+ DQB1*03:01/04/09/+

12 25D22 DRB1*14:01/04/07/+ DRB1*14:02/05/06/+ DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

13 25D26 DRB1*14:01/02/04/+ - DRB3*01//+ - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

14 25D27 DRB1*14:01/02/05/+ - DRB3*02:01/02/03/+ - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

15 25D29 DRB1*14:06/81/108 DRB1*14:54 DRB3*01/02/03 -

16 25D31 DRB1*14:01//+ - DRB3*01/02/03 -

17 25D35 DRB1*14:01/02/05/+ - DRB3*01/02/03 - DQB1*05:01/02/03/+ DQB1*03:01/04/09/+

18 25D37 DRB1*14:01/02/05/+ DRB1*14:01/02/05/+ DRB3*01/02/03 DRB3*01/02/03 DQB1*05:01/02/03/+ DQB1*03:01/04/09/+

19 25D40 DRB1*14:01/02/04/+ - DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

20 25D44 DRB1*14:01/02/04/+ - DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

21 25D47 DRB1*14:01/02/04/+ - DRB3*01:01/02/03/+ - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

22 25D49 DRB1*14:01/02/05/+ - DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

23 25D50 DRB1*14:01/02/04/+ - DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

24 25D51 DRB1*03/13/14 DRB1*14 DRB3*01/02/03 - DQB1*03 DQB1*05

25 25D52 DRB1*14:01/02/04/+ - DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

26 25D53 DRB1*14:01/02/04/+ - DRB3*01:01/02/03/+ DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

27 25D59 DRB1*14:01/02/04/+ - DRB3*01/02/03 - DQB1*03:01/04/09/+ DQB1*05:01/02/03/+

28 25D62 DRB1*14:01/02/03/+ - DRB3*01/02/03 -

29 25D63 DRB1*14:01/02/05/+ - DRB3*01/02/03 - DQB1*05:01/02/03/+ DQB1*03:01/04/09/+

 25D10 25D21 25D37 判定（表記 ? ）間違い

⇒ homo 接合と判定されていないためハイフン「 - 」

 25D12 25D17 25D19 25D47 25D53

HLA-DRB3*01:01/02/03/+ ⇒ DRB3*01?

HLA-DRB3*01/02/03 or HLA-DRB3*01:01/02:01/+

DRB5*01:01 ～ 14 DRB5*02:02 ～ 05

DRB5*01:01/02:02/+ or DRB5*01/02 DRB3*01:01 ～ 06/08 ～ 13

DRB3*02:01 ～ 17/19 ～ 25/28 DRB3*03:01 ～ 03

DRB3*01:01/02:01/+ or DRB3*01/02/03 DRB4*01:01 ～ 07

DRB4*02:01N ←JPN では PCR 陰性

DRB3*03:01N ←JPN では PCR 陰性

DRB4*01:01/02/03/+ or DRB4*01

• 試験結果ミスによるmiss assignは少なく 特にClassⅡのtypingに関しては良好な結果 であった

• 昨年に比較するとfalse positiveが散見され miss assignにつながっている

（特定の施設に同傾向）

• 同試薬であっても結果表記は様々であり

ambiguityの表記方法が統一していない