シリアンハムスターにおける低温ショックタンパク質の発現調節に関する研究
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(7) Ǘ ɒɁ. aCSF. artificial cerebrospinal fluid ¢ķȦȥʝƸ. CHA. N6-cyclohexyladenosine. CIRP. cold-inducible RNA-binding protein. HP ORF. . Hibernation protein open reading frame. RBM3. RNA-binding motif 3. RNP. ribonucleoprotein. RT-PCR. Reverse transcription polymerase chain reaction. SD. Standard deviation ƛƿ»ĸ. . .
(8) ȏɆ. |sOôDĚ9ýÞLj:°ƽŗĹŖOŧ/ʥęɴƽĿĘà%3Fƹɴ °ƽOĦ8¸03K-EʥĤĦ%-ƴÞOȍŧ'K"54KŹʥ yglGgʥ w769ɴ9IJċ!0Ơʖ:ʥ°ƽŗĹŖOŧ03 K8FNI(ʥʙɞ%ƪƽ¯'KǏĕ83ʥȬI°ƽO ǏĕƽĿ8ɤ8B4!K ʣ39ʤ"9ǍɘOÉǥ5ÉǥÞLj :ʥÉǥOȶ"58H03°ƽOȍŧ'K-E9Ww]ƷəOŠ3 K5ǎɄ#L3KÉǥOȶN7Ě9ýÞLj:ʥ±I9æĄ8HJ ƹɴ°ƽ¯%-Ēò8:ʥőŦÞOȍŧ'K"54(8ơ8ȭK´ ;ʥ|s4:°ƽ 27OăK5ʥŋÞǟ7őŦÞOȍŧ'K"54 (ʥĚ9Ēòő¼ƞOɝ"'"5ǧIL3Kʣ30ʤŹʥÉǥÞLj:ʥ ÉǥƁʥ°ƽ 10¨8ɮ'K8FNI(ʥőŦÞGùõ50-ǐú ƴÞOȍŧ'K"54K"9"5IʥÉǥÞLj:¯°ƽ8ȚKljƢ 7ƝƙO¸ƈ%3K5șILK ȯǟ8ʥ¯ƽƁ8:ʥȆȢÆ9ƚ7ɸȃ9ƴŖ¯%ʥ§ɖƝȣ¯ 'K"9"58HJâǜÎɻ83:ƚ7osƉŊ4KĨ ʉ8¯°ƽ:ʥőǸƔēʥȥʝů¾ʥȦƔēGȦę¾ʥȫĀǯƕʥƷàǺȳȵ 76I9 ŌOűÿ'K"5đ÷#L3Jʥ+9ƈÝŖɍſ#L3 Kʣ10, 20, 43, 48ʤB-ʥPGśƓǛ769ɬȶOŠÖ'K98F¯°ƽ ƈÝ4K5șIL3Kʣ57, 58ʤɺǼ7ʊĪ9ƣKîȣŖ9ʞǚŘ 4KŸǐÂȦǛ83Fʥ¯ɹȃ8HJńɝ"#LKȦʊĪOȑû'K -EȦB-:ÄɟOÊå'K¯°ƽǜƮưǡ#L11Kʣ16, 44ʤ%% 7Iʥ|sOôDÉǥOȶN7Ě9ýÞLj:ƗĿ8°ƽ¯'K5 ǐúƴÞOȍŧ47%-03ʥ¯°ƽ9osOãÎ8âǜŔǒ' . .
(9) K8ȭ037+"4ʥÉǥÞLj9ŧ1¯°ƽȚŖ9ZvhOɄſ%ʥ |sOôE-ʐÉǥÞLj8ɰǒ'K"54L;ʥ¯°ƽ9osOâǜ Ŕǒ4K5ƉŊ%- ƌǩDz4:ʥÉǥÞLj8. K¯°ƽȚŖ9ZvhOɄſ'Kǿí5%. 3ʥ¯ƽeo^l{^ɛ8Ǧǡ%-ól{^ɛ:¯ƽ769ȆȢgs g8H03ɑı#LKl{^ɛ~Q4Kʣ28, 63, 64ʤ¯ƽe o^l{^ɛ5%3óĦ#Lʥƙɩ7I=8Ɲȣ9ɄƑɬP4KF95 %3ʥCold-inducible RNA-binding proteinʣCIRPʤ5 RNA-binding motif3ʣRBM3ʤ ũ!ILK"LI9l{^ɛ:ʥN ƋǶ½8 RNA ȊòtTOʥC Ƌ Ƕ½8R]v_eontTOŧ1B-ʥCIRP é= RBM3 :ʥ |sʣ6, 35ʤG Ugʣ7, 36ʤʥosʣ9, 60ʤ76Ě9ÞLjDZ83 ƚ7ȫĀ8ǝǍ%3K"5đ÷#L3K#I8ưǡ'@"58ʥ IJċ!0Ơʖ4Kfgʣ56, 61ʤʥěċýÞLj4K^ ʣ11, 12ʤ5 0-Éǥ'KýÞLj8Ǧǡ%ʥȎȕǟ7ɲ¬ĠɄƑOȶ0-ǩDz83ʥ Ȧʥőȫʥȝȫé=Ǹț49Éǥ9 RBM3 9ǝǍ9žđ÷#L3K B-ʥCIRP é= RBM3 : RNA Ȋòl{^ɛ5%3ʥRNA 9 5’B-: 3’ʐȘ ɌʓčGRrvàʓč8Ȋò'K"58HJƚ7ɲ¬Ġ9ɠÈŌɕǻ 8ʂ%3K"5ǧIL3Kʣ29, 65ʤ´;ʥ UgG|s9ȆȢO ǒ-ǩDz4:ʥ3’ʐȘɌʓč8Ȋò%- CIRP é= RBM3 8HJdZrSR ɲ¬Ġ9Rrvàɕǻ#LKʣ28ʤB-ʥǾĶ4:ʥCIRP ȘɌ ɕǻGŢɹà²ǒ768ʂɪ'K19 mRNA 8Ȋò%ʥĤĦŖOʞE3 Kʣ58ʤRBM3 : miRNA 8Ȋò'K"58HJʥȎȕǟ7l{^ɛ9Ș Ɍ8ňʑO3K"5đ÷#L3Kʣ9ʤ#I8ʥ"LI9l{^ ɛ8:ʥTNF- GɭɹàƫȃʥŝɾĄĠ9Úĝ768ńɝ"#LKRs egOŠÖ'KƝȣK"5FſI5703Kʣ5, 25, 40, 62, 65ʤ¯ƽ . .
(10) 4Ďʘ%- Ug9ȦgTgƛƌ83:ʥRBM3 :ǬȉļȆȢ9ǐĢƉ ʁOłɾ'K5đ÷#L3Kʣ51ʤ"9H8ʥ¯ƽeo^l{^ɛ :¯ƽƁ8ǝǍOž#)K"5ʥB-ʥȆȢ¸ɗ݃OF1"5ʥ5> -19ljŐũ!ILK"LI9ljŐ8HJʥól{^ɛ:ƗĿ9¯°ƽ Ɓ83ʥǐúƴÞOȍŧ'K-E8¸ɗƝȣOǝŮ'KîȣŖK5ș ILKRBM3 813:Éǥ'KýÞLj8. KǝǍ9žđ÷#L. 3KF99ʥCIRP 813:Ɗ.đ÷7+"4ʥƌǩDz4:ʥ¯ƽe o^l{^ɛ9/ CIRP 8Ǧǡ%ʥÉǥÞLj8. KǝǍÞŜOſI8. 'K"5ʥB-ʥʐÉǥÞLj?9ŔǒîȣŖOȾÍ'"5Oǡǟ5%- ƌǩDz4:ÉǥÞLj5%3ʥeRyglOǒ-ÉǥÞLj8 3ʥȬǝǟ8ÉǥOɑǝ#)K-E8:ʥÉ9H7Ǩżé=ĭÊǏĕ4ʗ ȟ'K"5Œʒ4K"9H7Ǐĕ4ɉà'K"58HJʥÉǥÞLj :ʥȬǝǟ7Éǥ8ŒȽ7ƚ7ɲ¬ĠOĘà#)3KIJċ!0Ơʖ4 KeRygl:ʥĨʛĩÆ403FÊȱŁÆ4Ǩżé=ĭÊǏĕO ÇǍ'K"58HJȬǝǟ7ÉǥOɑǝ4KB-ʥÉǥƁ8:ÉǥÞLj9 ȦÆ4Rrxe A1 ìī°ɚƴà#LK"58HJʥ°ƽ¯'Kʣ50ʤ eRygl:ʥ¢DŽǟ8Rrxe A1 ìī°RcvgsʣCHAʤOȦ ĩÆš'K"58HJʥÉǥƚ9¯°ƽ?ɑı'KŹƮǪǴ#L3Kʣ31, 50ʤ"9H7ljŐOÕǒ%3ʥƌǩDz4:ÉǥÞLj5%3eRyg lOǒ- Ƿ 1 ǵ4:ʥeRygl8. K CIRP ɲ¬Ġ9ĻĹƁ5Ȭǝǟ7. ÉǥƁ9ǝǍOɄƑ%ʥǝǍ8ljŐǟ7ĘàĢĈ'K96ƖɈ%- Ƿ 2 ǵ4:ʥÉǥƁ9 CIRP ɲ¬Ġ9ǝǍɕǻOńɝ"'ȽĄOſI8'K -E8ʥÉǥƁ9ʗȟǏĕ5ʥ°ƽ¯8Ǧǡ%ʥƖɈ%-Ƿ 3 ǵ4:ʥÉ ǥƁ9 CIRP ɲ¬ĠǝǍƚŃ:ʐÉǥÞLj?Ŕǒ4K96OſI8 . .
(11) 'K-E8ʥÉǥ%7ÞLj4K Ug5os8. K CIRP ɲ¬Ġ9ǝǍ. OɄƑ%ʥÉǥÞLj5óƚ8Ęà'K6ƖɈ%-. . .
(12) Ĩ ʛƍŶé=ĨʛŹƮ. ĨʛÞLj Ĩʛ8:°ɺ 108 g-164 gʥ9-26 ɫʡ9ʋeRyglʥ°ɺ 34-44ʩʥ 8-12 ɫʡ9 ddY Ȁȋ9ʋ Ugé= °ɺ 257-388ʩʥ8-12 ɫʡ9 Sprague-Dawley ʣSDʤȀȋ9ʋosOǒ-'@39ÞLj:żƌ SLCʣʏĵ, żƌʤI ɜÃ%-ĨʛÞLj:¨9Ĩʛ8µ'KB4ʥĩƽ 22°CʥſƂøƉ 12 Ɓʁʦ 12 ƁʁʣſƉ 7 : 00-19 : 00ʤ8ɋĦ#L-Ǐĕ4ʗȟ%ʥʙ: MFʣYWl ɸƦ, Ɛ¡, żƌʤO-ʙ5ƫ:ȬǓ8Űë4KH8%- ƌǩDz8. KÞLjĨʛ:Ä3ʥĴʃěģÞLjĨʛğü«4ɐî#L-F9. 4JʥĴʃěģÞLjĨʛëŞȿǰ8ō03Ĩź%-ʣşɐǘð: 15096, 16030, 17015ʤ. ÉǥÞLj9²Í ƌǩDz4:ʥeRyglOĨʛĩÆ4Ȭǝǟ7Éǥ?ɑı%-ǭ IÉ9ǏĕOÇǍ'K-EʥeRyglO 4-59ÊȱŁÆ4ʥſƂ øƉ 8 Ɓʁʦ16 ƁʁʣſƉ 18 : 00- 2 : 00ʤ83ʗȟ%-¯ƽǏĕ4:eR ygl: 1 á(1¹Ôʗȟ5%-ŭɅ)(8°ƽOƾĦ'K-Eʥq segqʣDAS-7007; BioMedic Data Systems, rUVR, ǽćʤO ǒ-ygl8:sgmʣIPTT-300; BioMedic Data Systemsʤ OȡɴȞǔʜʁ9Ǡ8ČEɣP.°ƽƾĦ9ʉ8:ʥmegq ʣDAS-7007; BioMedic Data SystemsʤOygl8$'"58HJʥyg l8ŭɅ)(8°ƽOɇƾ%-ygl:ʥ1 ƇʁĭÊǏĕé=ſƂø Ɖ8ʚà#)3IʥʗŶOÖʅ%-ʗŶOÖʅ%3I: 10 : 00 é= 18 :. . .
(13) 00 8°ƽOƾĦ%-ygl9°ƽ 15¨57Jʥǡɀ8HKƴÞ¼ ƞʥŭɅ×ǁ8į%39œɳȶÞ9ƷĜé=°OE-°ßȾIL-Ēòʥ ÉǥOȶ03K5ÓĦ%-Éǥ²ÍƮ:ʥ4 á9yglOóƁ8ɰǒ% -Éǥ%-ygl:ÉǥȖ5%3Ŭƍ%-"LI:Ƿ 1 ǵ83Ȭǝǟ 7ÉǥƁ9 CIRP ɲ¬ĠǝǍOɕ@K-E9ɄƑ8³ǒ%-4 á9/ 2 B: 3 á9yglÉǥOȶ0-Ɓǃ4ʥó&lT_4Éǥ²ÍƮOɰ ǒ%3K98FNI(Éǥ%37yglOʥĭÊé=ǨżǏĕ ʗȟȖ5%3Ŭƍ%-"LI:Ƿ 2 ǵ83ʥ¯°ƽ5:7I7F99Éǥ OȶH7Ǐĕ?ɉà%-"58HK CIRP ɲ¬ĠǝǍ?9ňʑOɕ@KE9ɄƑ8³ǒ%-. R rxe A1 Rcvgs8HK¢DŽǟ¯°ƽ9²Í Ƿ 2 ǵ4:ʥÉǥÙ9ĘàOȉ(8Éǥƚ9¯°ƽıÃ9CO¢DŽǟ8ÇǍ %ʥCIRP ɲ¬Ġ9ǝǍ?9ňʑOƖɈ%-+9-EʥÉǥƁ9°ƽ¯8Ĭ %3KRrxe A1 ìī°9Rcvgs4K CHA Oǒ3¯°ƽ?ɑ ı%-yglO²Í%-229ǏĕƽĿ83ʗȟ%3JĭÊʚà%3 7ygl8ʥRrxe A1 Rcvgs N6-cyclohexyladenosineʣCHAʤ ʣSigma, h, ǽćʤOȦĩÆš%-Ü3ʥęɴIÊå'K"5 8H03ʥ¢DŽǟ8¯°ƽ?ıÃ%-ȦĩÆš9-Eʥygl8:[ TtZvʣAG-6; WTb, ¡ɵ, żƌʤOǖȔ%-ǖȔ9ʉ8:ʥy gl8`lʣ200 mg/kgʤé=\efʣ10 mg/kgʤOƺò%ʥȪȨ Æš'K"58HJÄɟʟɷOȶ0-ʟɷ%-yglʔɴ9ƩOÐJʥ ȦĆĦȹȔʣSR-6N; Narishige Scientific Laboratory, Ɛ¡, żƌʤ8ĆĦ%-y gl9ʔȰʜOʎÍ%ʥ_ 9ŀƛOƾĦ%-ygl9Ȧĉą. . .
(14) ʣ33ʤOèdž%ʥ[TtZvOï½Ȧĩʣ-0.3 mm AP, 2.6 mm L, 4.0 mm DVʤ 8ŪÃ%-ʎÍ%-ʔȰʜ8wfO 2 ƌČEɣCʥ[TtZv5wf OʥrlisOǒ3ĆĦ%-[TtZvÆ8:mZv ʣAD-6; WTbʤOǖȔ%-B-ʥ°ƽOƾĦ'K-Eʥ°ƽƾĦ ǒsgmOȪȨÆ8ČEɣP.ȷŌʥ¢DŽǟ7¯°ƽ8ɑı'K B4ʥygl8:ij75F 1 ɫʁ9ăŏƉʁO-CHA :¢ķȦȥ ʝƸʣaCSF; in mM: 13.9 NaCl, 0.5 KCl, 0.13 MgSO4, 0.24 CaCl2, 0.124 KH2PO4, 1.5 NaHCO3, 1 glucoseʤ8ǀɄ%-¢DŽǟ7¯°ƽ8ɑı'K-Eʥ T^T fV^eZvʣAMI-6; WTbʤO[TtZv8ŪÃ%ʥ 5 µL 9Ƹɼ57KH8ɕŵ%- 1 nmol 9 CHA Oš%-š9ʉ:y glO 228¸ƽ%-s8-°ƽ 32¦ɤB4¯%3 I:ÊȱŁ8ǯÞ#)-ygl9°ƽ¯%ʥŭɅ8į'KœɳêŔ ƷĜ%3I:ʥŧȌǟ8°ƽOƾĦ'K-E8°ƽɇƾǒʣML312; AD InstrumentsʤOȜɿI 2 cm 8ŪÃ%-4ɇƾ#LK°ƽ 10 8ɮ%-Ɓǃ4yglOĤƘƥ%ʥȈȓOŬƍ%-Ĩʛ:ʥs ƽĿʥÊȱŁÆƽĿʥsgmƽĿé=°ƽƽĿO Power Lab ʣAD Instrumentsʥetv, YgsRʤO¤%3b}l?ȉƁ ǟ8Ɋɽ%- B-ʥǷ 2 ǵ4:ʥ¯°ƽ9ňʑOʆ- CHA šȬ°8HK CIRP 9ɱŤ ǟgTe_?9ňʑOɕ@-+9-EʥCHA OšŌʥ°ƽO 378 ȍŧ%-ȖO²Í%-ƌĨʛ4: CHA Oš'KʉʥyglO 35-38 8¸ƽ%-s98-sgm8HJ°ƽOƾĦ%7 Iʥs9ƽĿOɰħɕǻ%-CHA Oš%3I 4 ƁʁŌ8ygl OĤƘƥ%ʥȈȓOŬƍ%-. . .
(15) T k~õÃʟɷȲ8HK¢DŽǟ¯°ƽ9²Í Ƿ 2 ǵ4:eRyglʥǷ 3 ǵ4: ddY Ȁ Ugé= SD Ȁos O³ǒ%ʥRrxeȀ9ɚƴàO¤#(ʥ¯ƽ+9F9 CIRP ǝǍ8éA' ňʑOƖɈ%-+9-EʥÞLj8ʥʟɷȲO²ǒ#)ʥ°ƽɕǻOȶN7 NJŜ4ęɴIÊå%-Ĩʛ8:ʥ229ǏĕƽĿ83ʗȟ%3JĭÊʚ à%37ÞLjOǒ-"9ŹƮ8HJʥÞLj:ÉǥƁ8ńɝ"#LK RrxeȀ9ɚƴàO¤#(ʥŅÖǟ8¯°ƽ?ıÃ#LKʟɷȲ8:° ÆI9ūÍ9ɨõÃʟɷȲ9Tk~ʣ~X®õÃʟɷƸ; Abbot japanʥƐ¡ʥżƌʤOǒ-°ƽOȜɿI 2 cm B4ŪÃ%ʥ ǢȩƽĿOƾĦ%-#I8osǒőʍąʍƗʣv^rSZʥƐ¡ʥ żƌʤOǒ3ʥĖĺĀʣDAM50 Differential Amplifier; World Precision Instrument, ~m, RZʤO¤%3őʍąOƾĦ%-°ƽO¯#)Kʉ8:ʥB (ʥĻŰƽOö'KÞLjOTk~ʣǂĿ 2ʢʥƵɨ 2.0 L/minʤOƵà #)3Ko^g8ǯÞ#)ʥÞà%-+%3ɨG8°ƽ5 őʍąOȹǦ%ʥñĨʛ8HKƽĿƎª?ǯÞ#)-ǯÞ8ʥ Tk~9õÃ: g^9ȹǦ8HJȶ0-ʣƵɨ 1.5 L/minʤĨʛ:ʥ ÞLj9Ǣȩƽʥőʍąé=ÊȱŁÆƽĿO Power Lab O¤%3b}l? ȉƁǟ8Ɋɽ%- ƌŹƮ4:ʥÞLj9°ƽ¯8őŦŴ¯'K+%3ʥʟɷO Ȍ. -Ēò8:Ɔȇǟ8őŦÞ¼ƞ'K+9-EʥőŦÞOŨƛ8Ƥʈǟ. 8Tk~9ǂĿO!-Tk~9ǂĿ:őŦʨ300 ă/min 9Ɓ 8Tk~ǂĿ 2ʢʥőŦ 200-300 ă/min 9Ɓ8 1.5ʢʥőŦ 150-200 ă/min 9Ɓ8 1.0ʢʥőŦ 100-150 ă/min 9Ɓ8 0.5ʢʥ őŦʧ100 ă/min 570-Ɓ 0ʢ 5%-ʣą 1ʤTk~ǂĿO!-ŌʥőŦŴž%-Ɓ8:ʥĿ . .
(16) Tk~ǂĿO!ʥÇĿőŦ¯%3ITk~ǂĿO!- Ƿ 2 ǵé=Ƿ 3 ǵ4:ʥɡĿ7¯°ƽ8HK CIRP ɲ¬Ġ9ǝǍ?9ňʑOɕ @K-Eʥ°ƽO 284ȍŧ%-ȖO²ŝ%-Ĩʛ4:ʥB( 258ɋĦ% -s94ÞLjOÊå%-ÞLj9°ƽ 288ɮ%-Is9ƽ ĿO 289ɋĦ8ĘƄ%-°ƽOɂĮ%7Iʥs9ƽĿOɰħɕǻ %-yglOǒ-Ĩʛ4: 2 Ɓʁʥ UgOǒ-Ĩʛ4: 1 Ɓʁʥ °ƽO 28
(17) 183ȍŧ%-2883ȍŧŌɨG8ĤƘƥ%ȈȓOŬƍ%- Ƿ 2 ǵ4:ʥygl83ʥɡĿ7¯°ƽ8HK CIRP ɲ¬ĠǝǍ9Ę àʥÉǥƚ9ƗĿ9¯°ƽ83Fȍŧ#LK96OƖɈ%-+ 9-Eʥ°ƽO 284ȍŧ%-Ōʥ10B4ŕÊ%-ȖO²ŝ%-Ĩʛ4:ʥ °ƽO 2883 2 Ɓʁȍŧ%-ŌʥɨG8 4-59ÊȱŁÆ?ǯÞ%ʥ°ƽ 1087KB4ÊåOȌ. -ygl9°ƽ 108ɮ%-Ɓǃ4ĤƘƥ. %ʥȈȓOŬƍ%- B-ʥǷ 2 ǵé=Ƿ 3 ǵ4:ʥɡĿ7ƽĿčOȃŽɧJɭKH8¯° ƽ?ɑı'K"58HK CIRP ɲ¬ĠǝǍ?9ňʑOɄƑ%-+9-Eʥ°ƽ O 10B-: 15B4ŕÊ%-ȖO²ŝ%-Ĩʛ4:ʥÞLj:ʟɷOõÃ# )Þà%-ŌʥɨG8 4-59ÊȱŁÆ?ǯÞ%ʥ°ƽ 10B-: 15 87KB4ÊåOȌ. -Ƿ 2 ǵ83ʥygl:°ƽ 108ɮ%-. Ɓǃ4ĤƘƥ%ʥȈȓOŬƍ%-Ƿ 3 ǵ83 Ug:ʥ°ƽ 158ɮ %-Ɓǃ4ĤƘƥ%- #I8ʥǷ 2 ǵ4:ʥɡĿ7¯°ƽOȉʛ%7Ēò9ʥƗĿ7¯°ƽ8H K CIRP ɲ¬ĠǝǍ?9ňʑOƖɈ%-+9-EʥyglOǒ3ʥ°ƽ O 10B4ŕÊŌ 6 Ɓʁȍŧ%-ȖO²ŝ%-Ĩʛ4:ʥygl:ʟɷ OõÃ#)Þà%-ŌʥɨG8 4-59ÊȱŁÆ?ǯÞ%ʥ°ƽO 10B4 Êå%-ŌʥÊȱŁƽĿO 8±48ɋĦ%ʥ¯°ƽNJŜOȍŧ%-6 ƁʁŌ8 . .
(18) ĤƘƥ%ʥȈȓOŬƍ%- Ƿ 3 ǵ4:ʥ Ug83ʥ¯°ƽ9ňʑOʆ-ʟɷȬ°8HK CIRP 9 ɱŤǟgTe_?9ňʑOɕ@-+9-EʥTk~OõÃŌʥ °ƽO 378ȍŧ%-ȖO²Í%-ƌĨʛ4:ʟɷOõÃ#)Þà%-Ōʥ ɨG8 UgO 35-388¸ƽ%-s98-°ƽOƾĦ%7 Iʥs9ƽĿOɰħɕǻ%-Tk~OõÃ%3I 2 ƁʁŌ8 UgOĤƘƥ%ʥȈȓOŬƍ%- . eRygl9ĻŰƽƁ8. KʇāÆƽĿ9ƾĦ. DžÌȔ9eRygl8Tk~OõÃ#)ʟɷ%-9/ʥƽĿ ɇƾǒ9sgmOʇāÆ8ŪÃ%-ʇāÆ4ɇƾ#L-ƽĿ:Ǿ Ķ9ƽĿOêƀ%3K5ș-B-ʥóƁ8ȪȨÆ8Ô9sgm OŪÃ%ʥȪė8ȒòǿOǒ3ĆĦ%-ȷŌ 1 ɫʁ:ăŏƉʁ5%- ĩƽ 22°CʥſƂøƉ 12 Ɓʁʦ12 ƁʁʣſƉ 7 : 00-19 : 00ʤ8ɋĦ#L-Ǐĕ 4ʗȟ%ʥʙ5ƫ:ȬǓ8Űë4KH8%-ʇāÆé=ȪȨÆ9ƽĿO 1 ż 1 ăʥſƉ83òɇ 7 żʁɇƾ%-. ĭÊǏĕ8ɉà%- Ug9²Í Ƿ 3 ǵ4:ʥ Ug8. KĭÊ×ǁ8HK CIRP ǝǍ?9ňʑOſI8'. K-E UgO 4-59ÊȱŁÆ4 1 ɫʁʗȟ%ʥĭÊǏĕʗȟȖO²ŝ%- ¯ƽǏĕ4 Ug: 1 á(1¹Ôʗȟ5%ʥʙ5ƫ:ȬǓ8Űë#)-. . .
(19) Ȉ ȓ9Ŭƍ ĻŰƽƁ9eRyglʥ Ugé=osʥÉǥƁ9eRy gl9ȦʥőȫʥȠʥȝȫʥȧȫé=ǾĶOŬƍ%-B-ʥ¢DŽǟ¯° ƽƁʥǨżĭÊƎª83ʗȟ%ʐÉǥƁ9ÞLj9Ȧé=őȫOŬƍ%-Ŭ ƍ9ʉ8:ʥÞLj8Tk~ʟɷOź%ʥʕÞȤIŲȵƥ%-ȫĀ: Krebs’s Ƹʣin mM: 118.05 NaCl,4.69 KCl, 2.52 CaCl2, 1.16 MgSO4, 1.16 NaH2PO4, 25.0 NaHCO3, 1.11 Glucoseʤ4Ʋƶ%-ŌʥƸ°dzȃ4ŕǁ8ÊË%-CIRP ɲ¬Ġ9ɄƑʣŌɥʤOȶB4:ʥ-809ÊȱŁÆ4¸Ģ%-. RNA ťÍé= cDNA 9²ŝ -8083¸Ģ%-ȦʥőȫʥȠʥȝȫʥȧȫé=ǾĶ9ȈȓLJȁ 50 mg Iʥ RNA ťÍ\osʣTRIZOL® Reagent; Thermo Fisher ScientificʥƐ¡ʥżƌB-: TRI Reagent; Molecular Research Center, Inc., YyTY, ǽćʤOǒ3ʥsl RNA OťÍ%-ɴ9d813: TURBOTMDNase enzymes ʣThermo Fisher ScientificʤOǒ3ʥƺÃ%-ax DNA 9ʆçOȶ0- ŎIL- RNA d9 260 nm 9Ưɾ8. KõÁĿOõÁĿɇ. ʣSpectrophotometer; HITACHIʥƐ¡ʥżƌʤ8HJƾĦ%-ƾĦ#L-õ ÁĿOF58 RNA 9ǂĿOǹÍ%-sl RNA 3 g IɦɠÈêŔO ȶʥcDNA Oòŝ%-ɦɠÈêŔ:ʥmT ʣThermo Fisher ScientificʤʥɦɠÈɸȃʣSuper Script® Reverse Transcriptase; Thermo Fisher ScientificʤOǒ3ʥd dT^ʣGeneAmp® PCR System 2700; Thermo Fisher Scientificʤ83ȶ0-. . .
(20) T 9²ŝ Ż8đ÷9K|sʣNM_001280.2ʤʥ UgʣNM_007705.2ʤʥos ʣNM_031147ʤ9 CIRP mRNA 9ÅɧɶÑOèș8%3ʥCIRP ɲ¬Ġ9ɠÈLj 9ƖÍ9-E8ǒKT O²ŝ%-+9-E8ʥ ř#LKʀĞb tOôDT O³ǒ%-Forward T O Cirp1ʣFʤʥreverse T O CirpʣRʤ5%3ȸ 1 8ǫ%-#I8ʥygl9 CIRP 9 open reading frame ʣORFʤÄɾOɄƑ'K-E8ʥ ř#LK ORF HJƵé= Ƶ9ɶÑ8ǣȺǟ7T O²ŝ%-T : Ugé=os9 ɶÑOèș8²ŝ%-ORF Äɾ9e^WgɄƑ8³ǒ%- forward T O Cirp2ʣFʤʥreverse T O Cirp2ʣRʤ5%3ȸ 1 8ǫ%-. PCR Ʈ Taq jʣPlatinum® Taq DNA Polymerase High FidelityʥT~q^ xfʤOǒ3ʥcDNA êŔƸ 1 L I PCR Oȶ0-d dT^ Oǒ3ʥÒƉĘŖ 94 2 Îʥ123 94 30 Ǯʥ55 30 Ǯʥ72 1 Î83ʥ28–35 dT^ʣŌɥʤʥ+9Ō 72 3 Î9ƽĿɋĦ83ǡǟ9ɶÑ Oŧ1 cDNA OĖĺ%-êŔƻ9Ƹ: 0.4 g/mL 9WnfU Ttʣu ZTqg^, ¡ɵ, żƌʤOôD 1.5%R[gaʣuZTqg^ʤO ǒ3ʍƪƱÞOȶ0-ʍƪƱÞ%-a8:ʥȅęȐsgTw lʣUVP Laboratory ProductsʥZ~XvRʥǽćB-: ChemiDoc™ XRS+ system; BIO RAD, Z~XvRʥǽćʤ8H03ȅęȐOdžİ%ʥêŔǐŝ LjOîɀà%-yglOǒ-Ĩʛ8. K PCR êŔ9dT^Ŵ:ʥ. cDNA 9ǓƏ9ȫĀ8H03Ħ5%-"LI9dT^ŴOɋĦ'K-E8ʥ 19d4 25-40 dT^9 PCR OȶʥƖÍ#LKzt9{l. . .
(21) 8ɯ7"5OǪɐ%-+%3ʥPCR ǑLj9ĖĺƃȐs8 ɮ#7dT^ŴOŬǒ%-ygl83:ʥȦʦ28 dT^ʥő ȫʦ29 dT^ʥȠʦ28 dT^ʥȝȫʦ35 dT^ʥȧȫʦ29 dT^ʥ ǾĶʦ30 dT^8ɋĦ%- Ugé=osǓƏ9 cDNA 83:Ä3 9ȫĀ4 30 dT^8ɋĦ%-. Ĕ ďɶÑ9Ʉɔ RT-PCR 8HJĖĺ%- CIRP cDNA 9ĔďɶÑOɄɔ%-ygl9Ļ Ĺ°ƽƁé=ÉǥƁʥ Ug9ĻŰƽƁ9őȫ8ǓƏ'K RNA I²ŝ%cDNA ƸOʥCirp1ʣFʤé=ʣRʤOǒ3Ėĺ#)-ʍƪƱÞ%-ŌʥR[ ga9ǡǟ9zt9ɴÎ9 PCR ǑLjOÏJÍ%ʥmT^se^ WgƮOȶ0-PCR ǑLj:eZƮʣNucleoSpin® Gel and PCR Clean-upʥMACHEREY-NAGEL, r, tTpʤ83ǾȻ%-ǾȻLj9 ɴOÇĿʍƪƱÞ%ʥǡǟ9zt9CǾȻ#L3K"5OǪɐ%-Ōʥ \osʣABI Prism® Big Dye® Terminator v3.1 Cycle Sequencing KitsʥThermo Fisher ScientificʤOǒ3e^WgêŔOȶ0-d dT^9ƽ ĿɋĦ:ʥ96 1 Îʥ123 96 10 Îʥ50 5 Îʥ60 4 ÎO 25 dT^ 83êŔOȶ0-+9ŌT Oʆç%ʥDNA ŷLJOǾȻ%-ǾȻ %-ŷLJ9Ʉɔ:ʥ n\}DNA e`dʣABI Prism 3100 Genetic Analyzer, Thermo Fisher ScientificʤO³ǒ%- +9ŌʥORF ÄɾOɄƑ'K-E8ʥ ř#LK ORF HJƵé=Ƶ9ɶ Ñ8ǣȺǟ7T Cirp2ʣFʤé= Cirp2ʣRʤ8H03Ėĺ%- PCR ǑLj 83e^WgɄƑOȶ0-Cirp1ʣFʤé=ʣRʤ8H03Ʉɔ%-ɶ ÑôBLK"5OǪɐ%ʥyglé= UgIƖÍ#L- cDNA ɶÑ. . .
(22) OƬĦ%-. Ǖ À9Ŵºàé=ȋɇÌǎ ŎIL- PCR ǕÀ: Image J k~sUVRʣNational Institutes of Health, t, ǽćʤOǒʥȴÁŅĿOztƧ8Ŵºà%-19d IŎIL-zt9/ʥ800 bp ¦ɤ9ztO long formʥB- 500 bp ¦ɤ9 ztO short form 5%-Long form 9ŴºO short form 9Ŵº4Û0-F9 O long form/short form 5%3ǹÍ%ʥǝǍɼ9ƨOǫ%- ŎIL-Ŵºrl:Ä3ĻĊº ± ƛƿ»ĸʣStandard deviation; SDʤ4ȸɊ %-2 Ȗʁ9ƖĦ:ʥStudent’s t ƖĦB-: Welch’s t ƖĦOǒ-2 Ȗ¨ 49ƖĦ:ʥDunnett’s ƖĦOǒ-P º < 0.05 9ĒòOȋɇģǟ8ƈŚ7 ĸK5%-ȋɇɄƑ8: Statcel4 k~sUVR ʣOMS, Đnj, żƌʤO ǒ-. . .
(23) ą 1ʦőŦŴ8HKTk~ǂĿ9ɕǻ. Tk~Oǒ3eRyglO¢DŽǟ8¯°ƽ?ɑı'Kʉ8 :ʥőŦŴOŨƛ8%3ʥõÃ#)KTk~9ǂĿOɕǻ%-őŦŴ ʨ300 ă/min 95:Tk~ǂĿO 2%5%-óƚ8ʥőŦŴ 200-300 ă/min 95 1.5%ʥőŦŴ 150-200 ă/min 95 1.0%ʥőŦŴ 100-150 ă/min 95 1.0%ʥőŦŴʧ100 ă/min 958: 0%5%-. .
(24) ȸ 1ʦĨʛ8³ǒ%-T . T :ʥ|sʥ Ugé=os9 CIRP mRNA 9ÅɧɶÑI²ŝ%- Cirp1 9 forward é= reverse T :+L,LʀĞbté=ȇƞbtO ôDCirp2 T : ř#LK ORF 9ę½9ɶÑ8Ȋò'KForward T OʣFʤʥreverse T OʣRʤ5ǫ'. .
(25) Ƿ 1ǵ. eRygl9ÉǥƁ8. K CIRP ɲ¬ĠǝǍ9ɄƑ. ÉǥÞLj:ʥÉǥ8ƗĿ9¯°ƽ57K8FNI(ʥȮúǟ7ʊĪ: 7ƌĨʛ4³ǒ%-eRygl83FʥÉǥ9¯°ƽƁ8 őȫ:ȿØƟ%ƳŋÞO¸03K"5ǧIL3Kʣ30ʤ"9"5 IʥeRygl9Éǥ8:ʥ¯ƽ8HKʊĪOăɳ'K-E9Ɲƙ ĢĈ%3K5șILKƌǩDz4:ʥʊĪOăɳ'K-E9Ɲƙ9<5 15%3 CIRP ɲ¬Ġ8Ǧǡ%-Éǥ8 CIRP ƝȣOǝŮ'K5'L;ʥ eRygl9Éǥ8:ĻĹƁ5ƨɢ%3 CIRP ɲ¬Ġ9ǝǍž %3K5șILKB-ʥeRygl9 CIRP ɲ¬Ġ9đ÷:Ɗ. 70--E CIRP ɲ¬Ġ9ǝǍK96OſI8'KŒȽ0 -+"4ʥƌǵ4:ʥeRygl8. KĻĹƁ9 CIRP ɲ¬Ġ9ǝ. ǍNJŜOſI8'K"5ʥB-ʥÉǥƁ8 CIRP ɲ¬Ġ9ǝǍĘà'K9 6OſI8'K"5Oǡǟ5%- . . .
(26) Ȋƒ. ĻŰƽȖ9eRygl8. K CIRP mRNA _9Ǫɐ. ĻŰƽȖ9eRygl9ȦʥőȫʥȠʥȝȫé=ȧȫ83ʥ RT-PCR Ʈ8HJ CIRP ɲ¬ĠǝǍOƖȄ%-5"MʥÄ39ȫĀ83ʥ1 Ȉ9T Iȁ 500 bpʥ700 bpʥ800 bp 9 3 19dTh9 PCR ǑLjĖ ĺ#Lʣą 2ʤ ȁ 500 bp 9ǑLjO short formʥ ȁ 700 bp 9ǑLjO medium formʥ ȁ 800 bp 9ǑLjO long form 5%-ax DNA 9ƺÃ9ƈDžOǪɐ'K-Eʥ ɴ9d813: DNase ÌǎO%- RNA ƸI²ŝ%- cDNA 4 PCR êŔOȶ0-DNase ÌǎŌ9 RNA I²ŝ%- cDNA d83Fʥ óƚ8 3 DZʖ9 PCR ǑLjĖĺ#L- őȫIĖĺ#L- PCR ǑLjOǒ3ʥe^WgɄƑOȶ0-5"Mʥ short form 9 ř#LK ORF ɶÑ:ʥ Ugʥosé=|s4đ÷#L3 K CIRP mRNA 9 ORF ɶÑ5ʞǣóŖOƈ%3-ǣóNj: Ug4 94ʢʥ os 93ʢʥ|s 89ʢ40-ʣą 3ʤB-ʥORF IRxɹɶÑO ř %-ygl5¥ÞLjDZ59RxɹɶÑ9ǣóNj:ʥ Ug 99.4ʢʥ os 98.8ʢʥ|s 95.9ʢ40-ʣą 4ʤ%-03ʥshort form 5%3ƖÍ #L- mRNA :ʥCIRP mRNA 9_4K5Ǫɐ#L- B-ʥȁ 800 bp 9 PCR ǑLjʣlong formʤ9 ř#LK ORF :ʥshort form 5 ORF 9ʀĞǃI 502 bp :ĥÄ8Ȯ%3-Źʥ3’ ƋǶ½9ɶÑ8 212 bp 9ĔďɶÑŪÃ#L3-|s CIRP mRNA 9W\kOèș8ʥW\ kƙɩO ř%-5"Mʥlong form 9ɶÑ:ʥyglCIRP mRNA _9Ƿ 6 W\k5Ƿ 7 W\k9ʁ8 CIRP mRNA 9Ts8-KɶÑ ŪÃ#L-Ň4K"5ſI570-ʣą 5ʤ"9Ƿ 6 W\k5Ƿ 7 W\k9ʁ8Ts8-KɶÑĢĈ'K5ƙɩ:ʥ Ugé= . .
(27) |s83đ÷9KɠÈzRsʣXM_006513165.1ʤé= ʣNM_001300829.2ʤ5Ȯ%3-ygl83ʥŪÃ#L-ɶÑ8 : stop btĢĈ%-%-03ʥlong form 9ɶÑI ř#LKRx ɹ:ʥCIRP 5: C-ƋǶ9Ǚ7KF94KîȣŖǫþ#L- ȁ 700 bp 9 PCR ǑLjʣmedium formʤ813:ʥmT^se^Wg ɄƑOɏC-ʥǑLj9ɼ short form G long form 5ƨɢ%3ijɼ4Jʥɶ ÑOƬĦ'K"5470-. É ǥȖ9eRygl8. K CIRP mRNA ǝǍ. ÉǥȖ9eRygl9ȦʥőȫʥȠʥȝȫé=ȧȫ8. K CIRP. mRNA 9ǝǍO RT-PCR Ʈ8HJɄƑ%-Ä39ȫĀ83ʥÉǥȖ4: ädTh9ȁ 500 bp 9 mRNA 8ǝǍʌȁ%ʥ+9¥9gTe_z Rs9ǝǍƼij'K"5ſI870-ʣą 6ʤĔďɶÑOɄƑ%-5 "Mʥȁ 500 bp 9ĖĺǑLj9ɶÑ:ʥĻŰƽȖ4ƖÍ#L- 3 DZʖ9ǑLj9 / short form 9F95ĥÄ8Ȯ%3-. ĻŰƽȖ5ÉǥȖ9eRygl8. K CIRP mRNA ǝǍ9ƨ. ɢ ĻŰƽȖé=ÉǥȖ9 CIRP mRNA ɄƑ83ƖÍ#L- short form é= long form 9zt9ȴÁŅĿOŴºà%ʥshort form 5 long form 9ǝǍɼ9ƨ O long form/short form 5%3ǹÍ%-ĻŰƽȖé=ÉǥȖ9ŴºOƨɢ% -5"MʥȦʥőȫʥȠʥȝȫé=ȧȫ83ʥÉǥȖ4:ĻŰƽȖ8į %3ʥlong form/short form :ƈŚ8Ƽij%-ʣą 7ʤ1BJĻŰƽȖ4: short form 55F8 long form ǝǍ%3-ʥÉǥȖ4: short form 8ǝǍʌȁ %-"5ſI870-. . .
(28) ą 2ʦĻŰƽȖ9eRygl8. K CIRP mRNA ɄƑ. ĻŰƽȖ9eRygl9őȫʥȦʥȠʥȝȫé=ȧȫ83ǝǍ%3 - CIRP mRNA 9 RT-PCR ǑLj9ʍƪƱÞÀOǫ'Ȧʦ28 dT^ʥőȫʦ 29 dT^ʥȠʦ28 dT^ʥȝȫʦ35 dT^ʥȧȫʦ29 dT^83 RT-PCR Oȶ0-ÈǤƜ9Ŵº: Z9ĔďįʣbpʤOǫ'ȁ 500 bp 9ǑLjO short formʥȁ 800 bp 9ǑLjO long form 5%-§ȸ5%3 3 á9ygl 8. KȊƒOǫ'. .
(29) ą 3ʦShort form bt'K CIRP 9_9 YrS_~ʣORFʤ8. KĔďɶÑ. eRygl9őȫ83ǝǍ%3- mRNA 9/ʥshort form 9Y rS_~ʣORFʤ9ɶÑOǫ'B-ʥŻ8đ÷9K U gʥosʥ|s9 CIRP 9 ORF ɶÑ5ƨɢ%-ygl9ĔďɶÑ5 Ugʥosʥ|s9ĔďɶÑ59ǣóNj:+L,Lʥ94%ʥ93%ʥ89%40 -. .
(30) ハムスター MASDEGKLFVGGLSFDTNEQALEQVFSKYGQISEVVVVKDR マウス MASDEGKLFVGGLSFDTNEQALEQVFSKYGQISEVVVVKDR ラット MASDEGKLFVGGLSFDTNEQALEQVFSKYGQISEVVVVKDR ヒト MASDEGKLFVGGLSFDTNEQSLEQVFSKYGQISEVVVVKDR. ETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQA ETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQA ETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQA ETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQA GKSSDNRARGYRGGSAGGRGFFRGGRSRGRGFSRGGGDRGY GKSSDNRSRGYRGGSAGGRGFFRGGRSRGRGFSRGGGDRGY GKSSDNRSRGYRGGSAGGRGFFRGGRSRGRGFSRGGGDWGY GKSSDNRSRGYRGGSAGGRGFFRGGRGRGRGFSRGGGDRGY GGGRFESRSGGYGGSRDYYASRSQGGSYGYRSSGGSYRDSY GGGRFESRSGGYGGSRDYYASRSQGGSYGYRSSGGSYRDSY GGGRFESRSGGYGGSRDYYASRSQGGSYGYRSSGGSYRDSY GGNRFESRSGGYGGSRDYYSSRSQSGGYSDRSSGGSYRDSY DSYATHNE DSYATHNE DSYATHNE DSYATHNE. 相同率 99.4 % 98.8 % 95.9 %. ą 4ʦShort form bt'K CIRP _9RxɹɶÑ. eRygl9őȫ83ǝǍ%3- mRNA 9/ʥshort form 9 ORF ɶÑI ř#LKRxɹɶÑOǫ'B-ʥŻ8đ÷9K Ugʥo sʥ|s9 CIRP 9RxɹɶÑ5ƨɢ%-ygl9RxɹɶÑ5 U gʥosʥ|s9RxɹɶÑ59ǣóNj:+L,Lʥ99.4%ʥ98.8%ʥ95.9% 40-. . .
(31) ą 5ʦeRygl8. K short form é= long form 9W\kƙɩ. |s CIRP mRNA 9W\kƙɩOèș8ʥshort form 5 long form 9ƙɩO ř %ʥƨɢ%-:ʀĞbtʥ:ȇƞbtOǫ'Ŵġ:ʀĞbt I9ĔďŴOǫ'_9ĂɃ4ǫ'ɴÎʥlong form 9/ short form 5 Ǚ7KɶÑ4K. .
(32) ą 6ʦÉǥȖ9eRygl8. K CIRP mRNA ǝǍɄƑ. eRygl9őȫʥȦʥȠʥȝȫé=ȧȫ83ʥÉǥ8ǝǍ%3 - CIRP mRNA 9 RT-PCR ǑLj9ʍƪƱÞÀOǫ'ą 2 5óƚ8Ȧʦ28 dT ^ʥőȫʦ29 dT^ʥȠʦ28 dT^ʥȝȫʦ35 dT^ʥȧȫʦ29 d T^83 RT-PCR Oȶ0-ÈǤƜ9Ŵº: Z9 bp Oǫ'§ȸ5% 3 3 á9ygl8. KȊƒOǫ'. .
(33) ą 7ʦĻŰƽȖ5ÉǥȖ9eRygl8. K. CIRP 9gTe_zRsǝǍ9ƨɢ. ĻŰƽȖ5ÉǥȖ9eRygl8. KgTe_zRs. 9ǝǍOĦɼǟ8Ɏ¶%-Short form 5 long form 9ǝǍɼ9ƨʣlong form/short formʤOĻĊº± SD 4ǫ'Ǟ9z:ĻŰƽȖʣn=5ʤʥʠ9z:Éǥ Ȗʣn=5ʤ9rlOǫ'ȋɇ8: Student’s t ƖĦB-: Welch’s t ƖĦOǒ -**:ĻŰƽȖ5ƨɢ%3ʥÉǥȖȋɇǟ8ƈŚ40-"5Oȸ' ʣP < 0.01ʤ. .
(34) șĮ. ¯ƽeo^l{^ɛ:ʥ¯ƽƁ8ǝǍž'K"5ſI5703 KÎĠ4Kʣ6, 7, 35, 36ʤB-ʥĎʘȆȢOǒ-ǩDz8HJʥ¯ƽe o^l{^ɛ8:ȆȢO¸ɗ'K²ǒK"5ſI8#L3Kʣ5, 25, 39, 61, 64ʤ+"4ʥƌǩDz4: CIRP ɲ¬Ġ8Ǧǡ%ʥÉǥÞLj9°Æ8 3F CIRP 9ǝǍĖÜ%3K94:75 ř%-+"4ƌǵ4:ʥ ÉǥÞLj4KeRygl9 CIRP ɲ¬Ġ9ǝǍOɄƑ%-ƌǵ9Ȋ ƒ8HJʥeRygl83ʥĻŰƽƁ8 CIRP _Obt 'K mRNA ǝǍ%3K"5ʥB-ʥȼŴ9gTe_zRsǝ Ǎ%3K"5ſI570-#I8ʥÉǥƁ8:Ɲȣǟ7 CIRP _ 8 mRNA 9ǝǍʌȁ'K"5ſI570-"9"5IʥÉǥÞLj9 ÉǥƁ8: CIRP ɲ¬Ġ9ǝǍ9äȂ7ĖÜ4:7ʥɱŤǟgTe_9 ɕǻO¤%-ʥǝǍZvhĢĈ'K"5ǫþ#L- ĻŰƽƁ9eRyglI:ij75F 3 DZʖ9 PCR ǑLjĖĺ #L-+"4ʥPCR ǑLj9ɶÑOɄɔ%-Ȋƒʥshort form 5Ħȗ%-ȁ 500 bp 9ǑLj:ʥ|sʥ Ugé=os83Ż8đ÷9K CIRP mRNA 5ʞ ǣóŖOǫ%-%-03ʥshort form 5%3ƖÍ#L-ɶÑ: CIRP 9y gl_4KîȣŖʞ|s CIRP 4:ʥRNA Ȋòɴ®4K ribonucleoproteinʣRNPʤ1 é= 2 9ɶÑGʥ+9ɶÑOôD RNA ȊòtT ʣRxɹ 1-84 ǘʤ9ɶÑƬĦ#L3Kʣ35ʤƌǩDz4ƬĦ%-eR ygl9 CIRP _I ř#LKRxɹɶÑ:ʥ|s CIRP 5ʥ RNP .. 47 RNA ȊòtTÄč4ěɴÎȮ%-ʣą 8ʤ%-0. 3ʥyglCIRP Fʥ|s CIRP 5óƚ8ʥRNA Ȋòl{^ɛ5%3Ɲȣ %3K5șILK . .
(35) ȯǟ8ʥl{^ɛ9ǝǍOž#)K-E8:ʥɲ¬Ġ9ɠÈO·ɬ' K"58HJ mRNA 9ɼOĖÜ#)ʥȘɌ#LKl{^ɛ9ɼOĖÜ#)K ZvhșILKŹʥÉǥƁ9eRygl:ʥCIRP mRNA 9ǝǍOĖÜ#)K-E8Œ(%FɠÈɼOĖÜ#)KŒȽ:75șIL K7*7IʥɱŤǟgTe_OĘà#)K"59C4 CIRP l{^ɛ 9ǝǍ9ÝNjOĖÜ#)ILKîȣŖKI4KÉǥƁ9H8ƽĿ 9¯'KNJƭ83:ʥƚ7ɸȃƴŖ¯'K-EʥɠÈ9Ę à:l{^ɛ9ĖÜ8BJĬ47îȣŖK+9-EʥɱŤǟ gTe_9ÏJƅ9C4ŒȽ7l{^ɛ9ǝǍÝNjOž#)KƝ ƙ4K5'L;ʥěĘƈǒ4K£ŌʥĨʉ8 CIRP ɲ¬Ġ9ɠÈé= CIRP mRNA 9ǝǍɼ813Ħɼ%ʥɱŤǟgTe_9ĘàgTe _zRs9ǝǍɼ869H7ňʑOéA'9ſI8'KŒȽK ƌǵ4ſI8%- long form 9ɶÑ:ʥshort form 9Ƿ 6 W\k5Ƿ 7 W \k9ʁ8ʥCIRP mRNA 9Ts8ǣņ'KɶÑŪÃ#LKŇ40 -"9"5IʥCIRP ɲ¬Ġ9ǝǍ8:ɱŤǟgTe_ȶNL3 Jʥlong form : CIRP ɲ¬Ġ8ǓƏ'KgTe_zRs4K5ș ILK+"4ʥlong form l{^ɛ?ȘɌ#L-Ēò9ƝȣO ƾ%- Long form bt%3K5 ř#LKRxɹɶÑ:ʥRNA ȊòtT 4 CIRP 5ĥÄ8Ȯ%-+9-Eʥlong form IȘɌ#LKl{^ɛF CIRP l{^ɛ5óƚ8 RNA Ȋòl{^ɛ4KîȣŖK%%ʥ long form bt'KRxɹɶÑ9 C ƋǶ: CIRP _9+L5:Ǚ7 K"5 ř#L-%-03ʥlong form IȘɌ#LKl{^ɛ:ʥCIRP 5: C ƋǶ½9Ǵ°ƙɩǙ7KîȣŖKCIRP ƴŖà'K-E8: nàK:ɹà#LK"5ŒȽ57Kʥ+L: CIRP 9 C ƋǶ4ɝ "K5đ÷Kʣ8ʤ+9-Eʥlong form IȘɌ#LKl{^ɛ:ʥRNA . .
(36) 8:Ȋò'KF99ʥnàK:ɹà#L(8 CIRP 5%39ƴŖO F-7"5Oǫþ%3K%-03ʥĻĹƁ8: long form IȘɌ#L Kl{^ɛ:Ɲȣǟ7 CIRP l{^ɛ9tusw[qS5%39ʼn ÛOƒ-%3K5șĮ%-1BJʥĻĹƁ8: CIRP 9ǝǍ:ɐEILKF 99ʥ+9Ɲȣ:ŠÖ#L3KîȣŖKŹʥÉǥƁ9eRy gl83ʥĻŰƽƁ8ĢĈ%3-ȼŴ9gTe_zRs :ɂĮ#L(ʥCIRP 9_9CǝǍ%3-"9"5IʥÉǥƁ8 :tusw[qS5%3¿RTk~X9ǝǍƼij'K-EʥƝ ȣǟ7 CIRP 9ƴŖà:ŠÖɄʆ#LK1BJʥĻĹƁ8: CIRP l{^ ɛ:ĢĈ'KF99Ɲȣ:ŠÖ#L3JʥÉǥƁ8: CIRP 9Ɲȣ9ŠÖO Ʉʆ'K5NJŜřĦ4Kʣą 9ʤ%-03ʥɱŤǟgTe_ Oǒ- CIRP ǝǍƝľ:ʥÉǥƁ8 CIRP l{^ɛ9ǝǍÝNjOž#)K .. 47ʥȃŽƝȣOǝŮ#)K-E8ƈǒ7Zvh4K5K. ʣą 9ʤ ƌǵ9ɺȽ7Ȋƒ5%3ʥĻŰƽƁ5ÉǥƁ8. KgTe_{l. 9ĘàʥőȫʥȦʥȠʥȝȫé=ȧȫ5ƚ7ȫĀIÅɧ8ĢĈ 'K"5ſI570-"9ȊƒIʥɱŤǟgTe_9Zvh :Ě9ȫĀ8Åɧ9F94K5șILKɱŤǟgTe_9ɕ ǻ8H03 CIRP l{^ɛƚ7ȫĀ83¸ɗ݃OǝŮ%3K5 ƉŊ4K ƌǵ4:ʥÉǥÞLj4KeRygl83ʥÉǥƁ8 CIRP ɲ¬ Ġ9ǝǍ9Ęàɝ"K"5O ř%ʥCIRP ɲ¬Ġ9ǝǍɄƑOȶ0-Ȋƒ 5%3ʥeRygl4:ĻĹƁé=ÉǥƁ96/I403F CIRP mRNA ǝǍ%3K"5ſI570-B-ʥĻĹƁ8:ȼŴ9g Te_zRsǝǍ%3JʥÉǥƁ8: CIRP mRNA ¨ę9gTe . .
(37) _zRs9ǝǍƼij%3-1BJʥĻĹƁIÉǥƁ8:ɱŤǟ gTe_9ɕǻɝ"JʥÉǥƁ8: CIRP mRNA 9ǝǍ8ʌȁ%3- #I8ʥgTe_zRs9ɶÑ:ʥCIRP 9tusw[qS4 K"5Oǫþ%3K"5IFʥÉǥƁ9ǝǍƚŃ: CIRP l{^ɛ9ǝ ǍÝNjé=ƝȣOʞEKîȣŖK. . .
(38) ą 8ʦShort form bt'KyglCIRP _5 |s CIRP l{^ɛ9ƝȣǟtT9ƨɢ. Short form IóĦ#L-eRygl9 CIRP _9ƙɩOʥ|s CIRP 4Ż8đ÷9KƝȣǟtT5ƨɢ%-|s CIRP 9ʀĞbt I 1-84 ǘǡ9Rxɹ: RNA ȊòtT4Klj8 RNPʣribonucleoproteinʤ 1 é= RNP2 : RNA Ȋòn~5%3ǐLjDZʁ4Ľ¸Ģ#L3KɶÑ4 K84 ǘǡ¨ʄ9Rxɹʓč:ʥ_eonʓč4KǞ9z: RxɹɶÑOǫ'|s CIRP 5yglCIRP 83ʥǙ7KRxɹ 9COȸɊ%ʥǞţ9ɴÎ:ǣó4K"5Oǫ'. .
(39) ą 9 : eRygl8. KɱŤǟgTe_9ɕǻOǒ-. CIRP l{^ɛǝǍZvh. ƌǩDzIșĮ4K CIRP l{^ɛǝǍƝƙ9©ɓOą4ǫ'ĻŰƽƁ 8:KǰĿ9ɠÈOȶʥȼŴ9gTe_zRs8Îų'K" 58HJ CIRP l{^9òŝɼGƝȣ¯ɕŵ#L3KÉǥƁ8: CIRP l{^ɛObt'K mRNA ?gTe_Oʌȁ#)K"58H03Ș ɌGƝȣOʞE3KîȣŖǫþ#LK"9Ɲƙ8H03ʥÉǥƁ9H 7ŕǁ7ƽĿĘà8ɨG8įŔ4K5ș-. .
(40) Œ 2ő. LiAlZdNPm)< CIRP *ƙúŀN_hBLlH*Æ¥Ž»*ēƃ. Œ 1 ő%+ƷŴľŀ(ńć) CIRP ƚ~ÌN_hBLlHƈŖ@< &Ć:&(!ńć)çƍ=< CIRP *ƙúŀN_hBLl HƈŖ*Ž»@Ć:)ƷIJ<&+Ʒxì*ĵijęŬ@ēƃƷƨ ń¢į.ðķ$ 5)ƝŽ% <%Čő%+Ʒńć*ƙúŀ N_hBLlH*Æ¥@çƍŽ»@Ć:)<&@Łŀ& LiAlZdNPm@ń<5)+ňăĎ}«-ØĴÄ)$ƯŪ <&ŽĜ=<Ƶ4, 19ƶ:)Ʒ*9
(41) (ĴÄ%ƯŪ<&)9; ń *ĩ&$`jelğ7ƚ~ÌľIJ)Ė (Æ¥çƍ=< &Á¶=$ <Ƶ2, 14, 41, 54ƶ 2Ʒńć)Ħ.Û<ƣ) +Ů%ASYLl A1 Õ@yLHVjïƬ% <Ƶ23, 50ƶ * 9
(42) (ń *Æ¥«-ńÛć*LHVj)ŅŁƷCIRP *ƙúŀN_ hBLlH)êƪ<*'
(43) @Ć:)<&&*5)ƷØ ĴÄ%ƯŪZdNPm2+ASYLl A1 ÕAJWNT@ŮÓ øqĦ.ƇÛZdNPm* CIRP *N_hBLlHľIJ@Ɓď :)ƷƳƜŹ@ķ $Ħ.ƇÛ<&)9;Ʒń)ơq<A SYLlŘ*LHVj@yƷĦ*6*)9!$ńĖ* CIRP *ƙú ŀN_hBLlH@IJ%<'
(44) ēƃ. . .
(45) ŝĐ. Ø«-ňăĎ}ƯŪ)9< CIRP mRNA *N_hBLlHľIJ.*êƪ ØĴÄ«-ňăĎ}ƯŪ CIRP *ƙúŀN_hBLlHƈŖ)êƪ@q <*'
(46) @Ɓď<5Ʒń$ ( 6**Ø«-ňăĴÄ%Ɵ ĉƠƯŪƷØ«-ňăĴÄƯŪť*ZdNPm@ķ $ CIRP mRNA * Ɓď@Ż!=:*ZdNPm&²PBclH%Ø«-ňăĎ}%Ư ŪZdNPm* 50%|o+ń@ŜƱńZdNPm+Œ 1 ő ) $Ʒń&$Ɓď) ķRT-PCR *ŝĐƷØ«-ňăĴ Ä%ƯŪť*ZdNPm*Ů«-îų: 3 Č*[lUē=Ƶ¼ 10ƶ2Ʒshort form & long form *ľIJƞ*Ě@ŕƷ¼ 11 )2&5 Ø«-ňăĴÄƯŪť&áàĦť*ZdNPm) $ƷĈõ(ß+Ɔ5 :=(!. ASYLl A1 ÕAJWNTƵCHAƶ@ķ wĬŀ(Ħ)9< CIRP mRNA *N_hBLlHľIJ.*êƪ 7 ¦*ZdNPm) $ƷCHA *ŮÓøq&żƩ:*©@ś3± ?<&)9!$Ħ.ƇÛ/$*ZdNPm*Ħ*ŜćŀÆ ¥@¼ 12 )ŋ4 ¦*ZdNPm%+ƷCHA øq:ƷĦ 10) Ɨ<2%) 350-465 Ƶ400±42 n=4ƶ@ŽÒƣ*Ŵľŀ(ń) $*ĦƗćƠ6ř 500 Ŏã% <Ƶ19, 50ƶ5Ʒ=:*ZdN Pm*ĦpƔã+Ŵľŀ(ń&Ʈ$ :)Ʒ=:*ZdN Pm+Ŵľŀ(ń&²Ė)Ʒ@2<5Ë£@µ$ nĂƷ3 ¦* ZdNPm+ƷĚƐŀŚĄ Ħp@ŋ$;Ʒ10)Ɨ<2%) !ćƠ+ 165-215 Ƶ193±20 n=3ƶ% !Ħp)ćƠ@Ž . .
(47) 4 ¦@ Slow ťƷŚĄĦp 3 ¦@ Fast ť&Slow ť& Fast ť * 10ƗćƠ)+ŞƂŀ)Ĉõ(ßƆ5:=RT-PCR )9;ƷĦ pƔã)?:/$*ZdNPm) $Ʒîų%+ short form *3 ē=Ƶ¼ 13ƶŮ) $+ƷÒƱ) 7 ¦*ZdNPm*
(48). Ʒ. 10Ɨ2%*ćƠĈõ)Ɵ! Slow ť%+Ʒshort form .*ƥřƆ5 :=Ƶ¼ 13 slow ťƶnĂƷ Fast ť*ZdNPm%+ƷŮ) $+ short form &&6) long form *ľIJƆ5:=Ƶ¼ 13 fast ťƶnĂƷCHA @ø qìƷĦ@ 37)şûť%+Ʒîų«-Ů) $ƷƙúŀN_hBL lH*\Pml*Æ¥+çƍ=(!Ƶ¼ 14, 15ƶ 2Ʒshort form & long form *ľIJƞ*Ě@ŕƷáàĦť*ZdNP m&ĚƐîų) $+ƷCHA @øqĦ&(! Slow ť«Fast ť%ƷĈõ(ßƆ5:=Ƶ¼ 15ƶŮ) $ƷSlow ť%+îų&² Ė)ƷáàĦť&ĚƐŞƂÎŀ)Ĉõ)Æ¥$ Ƶ¼ 15 slow ťƶ n ĂƷCHA @øqĚƐŀŚĄĦp Fast ť%+ƷŮ)< long form/ short form Ě+áàĦť&ĚƐ$Ĉõ(ß+Ɔ5:=(!Ƶ¼ 15 fast ťƶ2ƷCHA @øqìƷĦ@ 37)şûť) $+îųƷŮ &6)ƷáàĦť& long form/ short form Ě)ß+(!. Ə ã(Ħ)9< CIRP mRNA *N_hBLlHľIJ.*êƪ CHA @ķ wĬŀĦ%+ƷŮ) $N_hBLlH*ľIJ) 2 "* \PmlƆ5:=$Ʒĺ(<ľIJ\Pml@ŋZdNPm+Ʒ Ħ*pƔã)6Ƙ ;Ʒ8!;&Ħp@ŋ±)*3 ńĖ* CIRP *ƙúŀN_hBLlHƆ5:=8!;&Ħp @±)+ƷŚĄp±9;Ɵ ćƠƷƏã(ĦãÀ@ŜƱ <!$ƷƏã(Ħ*6* CIRP ƚ~Ì@ƈŖ<®Ŭò@ēƃ . .
(49) %ƷASYLl@y( ĂĠ% ;Ʒ"ƷÇƛ*Ħã@ƈŖ <&)9;Ʒ¢į*Ħ@þ<&%<ƳƜŹ@ķ ĦƇÛ Ġ@ķ 28zƒ2%*Ħ@Əã*ĦƷ10*Ħ@Ĕã* Ħ&ÑŦƷƏã(Ħ)9!$ CIRP Æ¥<*'
(50) ƈ/22 *ÓĦ%ƯŪ$ ZdNPm@ƷƳƜ&©*ś3±?%wĬŀ) Ħ.ƇÛČĂĠ)9<łűĦ*Æ¥@¼ 16 )ŋwĬŀ)Ħ@ 28%şûť%+Ʒîų«-Ů) $ƷN_hBLlH[iAlT*
(51) short form £&(<ńć*\Pmlē=Ƶ¼ 17 Kl_j 3ƶ2Ʒshort form & long form *ľIJƞ*Ě@ŕ&>ƷáàĦť *ZdNPm&ĚƐƷĈõ(ßƆ5:=Ƶ¼ 18 Kl_j 3ƶĦ@ 28%şûìƷ102%ñť) $6²Ė)Ʒńť&Ʈ ƷN_hBLlH short form .ƥř\Pmlē=Ƶ¼ 17 Kl _j 4ƶ2ƷáàĦť&ĚƐƷlong form/ short form Ě)" $6ŞƂÎ ŀ)Ĉõ)Æ¥$ <&Ɔ5:=Ƶ¼ 18 Kl_j 4ƶ=:*Zd NPm*łűĦ%ħÑĦ 28.Ɨ<2%*ćƠ+ƷBO^jhl @´Ê5$: 40-70 Ƶ51±8 , n=10ƶì% ! 2ƷZdNPm*Ħ@ 28%şûƷñƔ) 102%p ±) CIRP *N_hBLlHÆ¥<'
(52) @ēƃZdNPm) BO^jhl@´ìƔ7) 4)şûŸä)ō¢& >Ʒ80-185 Ƶ111±31 , n=9ƶì)Ħ 10)Ɨ*ĂĠ%+ 3228*ĦãÀ)ν<ćƠ+ 8-10 Ƶ8.9 ± 1.0 , n=9ƶ!Ƶ¼ 16ƶ Ħ@ 102%ñť%+Ʒîų«-Ů) $Ʒshort form .*ƥř+Ć :)Ɔ5:=(!Ƶ¼ 17 Kl_j 1ƶ Long form/ short form Ě)" $ 6ƷáàĦť&ĚƐƷŞƂÎŀ)Ĉõ)Æ¥$ <&+Ɔ5:=( !Ƶ¼ 18 Kl_j 1ƶ2ƷƟćƠ*Ĕã*Ħ@ŜƱ<&)9; . .
(53) CIRP *ƙúŀN_hBLlHÆ¥<*'
(54) @ƈ/Ħ@ 102 %ñì 6 ćƠşûť%+Ʒîų«-Ů) $ƷN_hBLlH[iA lT* short form .*ƥř+Ć:)Ɔ5:=(!Ƶ¼ 17 Kl_j 2ƶ Long form/ short form Ě)" $6ƷáàĦć&ĚƐƷĈõ)Æ¥$ < &+Ɔ5:=(!Ƶ¼ 18 Kl_j 2ƶ ŗ Ý*Ħã«- CIRP mRNA *N_hBLlHľIJ ÓĦ 22%ƯŪLiAlZdNPm*ŗÝ*Ħã@ƷƢº)ƄŤ ĦƂħķThlNalQm)9;Ƃħ&>Ʒ32.2±3.0% !Ų Ű)ƄŤThlNalQm)9;ƂħŲŰĦã+Ʒ36.6±0.8%. !2ƷRT-PCR )9; CIRP mRNA *N_hBLlHľIJ@Ɓď &>ƷîųƷŮƷūƷũų«-ůų*ľIJ&+ĺ(;ƷŗÝ%+áàĦť ) $6 long form *ľIJ+Ü(Ʒ short form ÈľIJ$ Ƶ¼ 19ƶ 2ƷáàĦť&ńť)N_hBLlH\Pml*Ƙ +Ɔ5:= (!Ƶ¼ 19ƶLong form/ short form Ě)" $6ƷáàĦť&ĚƐƷ ŞƂÎŀ)Ĉõ)Æ¥$ <&+Ɔ5:=(!Ƶ¼ 20ƶ. . .
(55) ¼ 10ƸØ«-ňăĴÄƯŪť*ZdNPm)< CIRP mRNA ľIJƁď. Ø«-ňăĴÄƯŪť*LiAlZdNPm)<îų«-Ů)ľIJ$ CIRP mRNA * RT-PCR Ķį*Ʀěġ¢@ŋŮƸ28 KBGjƷî ųƸ29 KBGj)$ RT-PCR @Ż!ŃĘ*ÿ+bmDm* bp @ŋ {ż&$ 3 ¦*ZdNPm)<ŝĐ@ŋĚƐÚƊ&$áà Ħť«-ńťƷ== 1 ¦*ŝĐ@Þ)ŋ. .
(56) ¼ 11ƸØ«-ňăĴÄƯŪť)<N_hBLlH[iAlTľIJ*Æ¥. °*îų«-Ů)< short form & long form *ľIJƞ*ĚƵlong form/short formƶ@ŕáàĦťƵn=5ƶƷńťƵn=5ƶ«-Ø«ňăĴÄƯŪťƵn=7ƶ @á¾± SD %ŋÈƝĚƐ)+ Dunnett’s Ġ@ķ ƷáàĦť&==*ť*@ĚƐ**+áàĦť&ĚƐ$Ş Ƃŀ)Ĉõ% !&@żƵP < 0.01ƶ. .
(57) ¼ 12ƸCHA @ķ $Ħ.ƇÛLiAlZdNPm)< Ħ*ŜćŀÆ¥. LiAlZdNPm*ŮÓ)ASYLl A1 ÕAJWNTƵCHAƶ@ø q$:*Ħ*ŜćŀÆ¥@ŋƴÃ;*bmG%ŋťƵFast ťƶ &Ŀù*bmG%ŋťƵSlow ťƶ*Ħ 10)Ɨ<2%*ćƠ )+Ĉõ(ß !. .
(58) ¼ 13ƸCHA @ķ $Ħ.ƇÛLiAlZdNPm)< CIRP mRNA ľIJƁď. CHA @øq©LiAlZdNPm)<îų«-Ů)ľIJ$ CIRP mRNA * RT-PCR Ķį*Ʀěġ¢@ŋŮƸ28 KBGjƷîųƸ29 KBGj)$ RT-PCR @Ż!ŃĘ*ÿ+bmDm* bp @ŋĚƐ &$áàĦć«-ńć* PCR @ŋSlow ť+ 10Ɨ2%*ćƠ Ĉõ)Ɵ!ťƷ2ƷFast ť+ 10Ɨ2%*ćƠň!ť% < PCR Kl_jp*bmG+Ʒ¼ 12 ) $Ħp@ŋ&²n%. <ĚƐÚƊ&$áàĦť* PCR @ŋ. .
(59) ¼ 14ƸCHA øqìĦ@ 37)şûť)< CIRP mRNA ľIJƁď. CHA øqìƷĦ@ 37% 4 ćƠşûLiAlZdNPm)<Ʒî ų«-Ů)ľIJ$ CIRP mRNA * RT-PCR Ķį*Ʀěġ¢@ŋ ŮƸ28 KBGjƷîųƸ29 KBGj)$ RT-PCR @Ż!ŃĘ*ÿ+ bmDm* bp @ŋ{ż&$ 2 ¦*ZdNPm)<ŝĐ@ŋĚ ƐÚƊ&$áàĦť«-ńť*ŝĐ@Þ)ŋ. .
(60) ¼ 15ƸCHA øq)9< CIRP N_hBLlH[iAlTľIJ*Æ¥. °*îų«-Ů)< short form & long form *ľIJƞ*ĚƵlong form/short formƶ@ŕáàĦťƵn=5ƶ&ƷńťƵn=5ƶ ƷSlow ť Ƶn=4ƶ ƷFast ťƵn=3ƶ«- CHA @øqìƷĦ@ 37)şûťƵn=4ƶ @á¾ SD %ŋÈƝĚƐ)+ Dunnett’s Ġ@ķ ƷáàĦť&= =*ť*@ĚƐ**+áàĦť&ĚƐ$ŞƂŀ)Ĉõ% !& @żƵP < 0.01ƶ. .
(61) ¼ 16ƸBO^jhl@ķ $Ħ.ƇÛLiAlZdNPm)< Ħ*ŜćŀÆ¥. LiAlZdNPm)BO^jhl@´©Â±*Ħ*ŜćŀÆ ¥ŋĦ@ 28%şûť* 1 @ƷĦ@ 28%şûìƷ 102%ñť* 1 @%bmGHh^%ŋ2ƷĦ@ 10 2%ñť* 1 @ƷĦ@ 102%ñì 6 ćƠşûť* 1 @ %bmGHh^%ŋ¼r*ņ¨+ZdNPm*śţ@üčPB clH@ŋ$;ƷĹ¯+¼ 17 «- 18 *Kl_jĹ¯&nŵ$ <. .
(62) ¼ 17ƸBO^jhl@ķ $Ħ.ƇÛLiAlZdNPm)< CIRP mRNA ľIJƁď. BO^jhl@ķ $wĬŀ)Ħ&LiAlZdNPm)<Ʒî ų«-Ů)ľIJ$ CIRP mRNA * RT-PCR Ķį*Ʀěġ¢@ŋ ŮƸ28 KBGjƷîųƸ29 KBGj)$ RT-PCR @Ż!ŃĘ*ÿ+ bmDm* bp @ŋKl_j 1 +ƷĦ@ 102%ñťƷKl_j 2 +Ħ@ 102%ñì 6 ćƠşûťƷKl_j 3 +Ħ@ 28%şû ťƷKl_j 4 +Ħ@ 28%şûì 102%ñť:Ʒ =={ż* 1 @ŋĚƐÚƊ&$áàĦť«-ńť*ŝĐ@Þ) ŋ. .
(63) ¼ 18ƸBO^jhl@ķ $Ħ.ƇÛLiAlZdNPm)< CIRP N_hBLlH[iAlTľIJ*Æ¥. °*îų«-Ů)< short form & long form *ľIJƞ*ĚƵlong form/short formƶ@ŕáàĦťƵn=4ƶ&ƷńťƵn=4ƶ ƷĦ@ 102%ñťƵKl_j 1Ƹn=4ƶƷĦ@ 102%ñì 6 ćƠşû ťƵKl_j 2Ƹn=5ƶƷĦ@ 28%şûťƵKl_j 3Ƹn=5ƶ«- Ħ@ 28%şûì 102%ñťƵKl_j 4Ƹn=5ƶ@á¾ SD %ŋÈƝĚƐ)+ Dunnett’s Ġ@ķ ƷáàĦť&==*ť*@Ě Ɛ**+áàĦť&ĚƐ$ŞƂŀ)Ĉõ% !&@żƵP < 0.01ƶ. .
(64) ¼ 19ƸáàĦť«-ńť*LiAlZdNPm)< ŗÝ* CIRP mRNA ľIJƁď. áàĦť«-ńť*LiAlZdNPm) $ŗÝ)ľIJ$ CIRP mRNA * RT-PCR Ķį*Ʀěġ¢@ŋRT-PCR + 30 KBGj)$ Ż!ŃĘ*ÿ+bmDm* bp @ŋ{ż&$°ť 3 ¦*ZdN Pm)<ŝĐ@ŋ. .
(65) ¼ 20ƸáàĦť&ńť*LiAlZdNPm)<ŗÝ* CIRP *N_hBLlH[iAlTľIJ*ĚƐ. áàĦť&ńť*LiAlZdNPm) $ŗÝ)ľIJ$ < short form & long form *ľIJƞ*ĚƵlong form/short formƶ@á¾± SD %ŋ Ŀ*[m+áàĦťƵn=5ƶƷƴ*[m+ńťƵn=5ƶ*SmP@ŋŞ Ƃ)+ Student’s t ēÑ@ķ **+áàĦť&ĚƐ$ƷńťŞƂŀ )Ĉõ% !&@żƵP < 0.01ƶĚƐ&$Ʒîų)<áàĦć «-ńć*SmP@ŋ. .
(66) ŧÙ. Œ 2 ő%+ƷŴľŀ(ńć)Ɔ5:= CIRP ƚ~Ì*ƙúŀN_hBL lH*Æ¥@çƍŽ»@Ć:)<&@Łŀ&*5Ʒ ń¢į% <LiAlZdNPm@ķ $Ė (Ď}p% CIRP *N_hBL lHľIJ*Ɓď@Ż!*ŝĐƷÒƣ)Ħ&(!$ ( ±)+Ʒ ØĴÄ«-ňăĎ}.*ƫ¥7ASYLl A1 Õ@yLHVj)9 !$ƷƙúŀN_hBLlH*Æ¥+çƍ=( &Ć:&(! nĂƷƏã(Ħ@§(ćƠşû<&%ƷCIRP *ƙúŀN_h BLlH*ľIJ\PmlńĖ)Æ¥Ŵľŀ(ń) $ƷLiA lZdNPm+8!;&Ħp@µ<5ƷƏã(Ħ@ÿćƠ ŜƱ$ <&ŧ :=<*&:ƷLiAlZdNPm*Ŵľŀń ) $6ƷƏã(Ħ)9;ƙúŀN_hBLlHÆ¥<®Ŭòŋ ¸=< =2%Ʒńć*Ħ)Ú$Ũò@ıí<5)+Ʒń *ĉƠ )<Ė (ų¹%*ƚ~Ì*ľIJÆ¥«-=)
(67) ieSilHïŽ % <&ŧ :=$ ,Ʒń¢į%+Ħp)Ő!$ƷîŔ* FfR_MflGLgl@éö<IXELl 43 *ľIJoąƷîŔ*¬ š)ơq<TkaWl C *ľIJp$ <œ*Á¶ <Ƶ2, 14, 41ƶ !$Čʼnŏ) $6ƷCIRP *ƙúŀN_hBLlH*Æ¥Ʒń ć*Ħp)Ő!$çƍ=$ <®Ŭò@ēƃƷŝĐ &$ƷØ«-ňăĎ}%*ƯŪ*3%ƷÒƣ)Ħp*ƍ!$ ( ZdNPm) $+Ʒńć)ƀÙ=9
(68) ( short form * CIRP mRNA .*ƥř+ŊƆ=(!²ĉƠƷ²ĴÄ%ƯŪZdNPm ń$ <&:Ʒ*ĴÄ:q :=<Ħãī«-ī+Ʒ <Ŏ . .
(69) ãƎ*`jel7ŌŜŀ(Æ¥@6:$ <+% <*ÒƱ) ƷØ«-ňăĎ}%ƯŪZdNPmń *ĩ@Ðu$ '
(70) )" $+Ć:%+( 6**ƷÜ(&6ƛŀ)+ń< 5*Æ¥@ŜƱ$ <&ŧ :=<2ƷCHA 2+BO^jhl@ķ wĬŀ(ĂĠ%Ħ@pÒƱ%+ƷØĴÄ%ƯŪ$ ( Zd NPm@ķ 6**ƷĦ*Ď})9!$+ CIRP *N_hBLlH+ń Ė)Æ¥$ <*&:6ƷØ«-ňăĎ}+ CIRP *N_hBL lH*Æ¥)ïƬ%+( &ŋ¸= LiAlZdNPm*Ŵľŀ(ń)+Ʒſâpƛ*ASYLl A1 ÕÅ¡<&ƝŽ% <&Á¶ <Ħp*5)ƷASYL l A1 Õ+ Gi źĿë¿Õ&$Ʒſâpƛ) $ċĒ:*Ĝî òLHVj@÷ƷĮĶĵęĕ@÷<Ƶ50ƶ *Ňž+ƷŴľŀ( ń@Ż!$ <ZdNPm7MiN*Ů)ASYLl A1 Õ*AlPJ WNT@øq<&Ŵľŀ(ĦprĀ<&
(71) ÒƱ)9!$ƅĆ= $ <Ƶ23, 50ƶ*9
(72) )ƷŴľŀ(ń*Ħ.*Û)+ASYLl Ř*ƋĢ¥ƝŽ% <&:ƷČʼnŏ) $6 CIRP *ƙúŀN_hB LlH*Æ¥)ASYLl)9<êƪ <&vô%Ʒ ASYLl A1 Õ@İĺŀ)Ģò¥<ķƞ* CHA @ŮÓøqƷLiAlZdN Pm@ńĖ*Ħ.ƇÛ<ĂĠƵ31ƶ@ķ $ CIRP ƚ~Ì*Ɓď@Ż !îų) $+ CHA @øqĦ&(!±)+Ʒvô&nŵ $ CIRP *N_hBLlH+Æ¥%Ʒ6ASYLlŘ*ƋĢ¥ *6*êƪ<&=,ƷĦ)ƇÛ<&(Ʒ37)şû± %6 CIRP *N_hBLlHÆ¥<&vôƷCHA @øqì) 37)şû±)+ CIRP *N_hBLlHÆ¥+Ɔ5:=(!" 2;ƷĦ&(!±)+ CIRP *N_hBLlH+Æ¥<6**ƷA . .
(73) SYLl A1 ÕLHVj*rđ%*Ģò¥*3%+ƙúŀN_hBLlH *Æ¥+çƍ=( &Ć:&(! CHA @ŮÓ)øqĦ&(!ZdNPm*Ů) $ƷĦp Ĉõ)Ą ZdNPm%+ƷCIRP *N_hBLlH+ńĖ*Æ¥@ƍ (!Ŷ·Ĥ &)ƷbCN*ģưNhBNėČ@ķ ʼnŏ) $Ʒ33zƒ*Ħp%+ CIRP mRNA *ľIJÅ¡<nĂƷ17zƒ%+ ľIJÅ¡( &
(74) Á¶ <Ƶ52ƶ*&:ƷĦpƔã*Ƙ ƙúŀN_hBLlH)êƪ@«1$ <®Ŭò <&ŧ Ħ pĄ ±ƷƏã(ĦÀ%*νćƠ+ňƷĦpƕ ±)+ Əã(ĦÀ%*νćƠ+Ɵ "2;ƷƏã(ĦãÀ%*νćƠ)9! $ƙúŀN_hBLlH*Æ¥*ĈĭĝÑ$ <®Ŭò < ĦpƔãĄ!ZdNPm%+ƷŮ&îų:ĺ(<N_hBLl H\Pmlē=*&+ƷŮ&îų*Ħãª×)+ĺ(! 5% <&ŧ :=<ŚĄĦ@pZdNPm+Ŵľŀ(ńć *9
(75) (s2!Ë£@µ$:Ʒ-!£% !ČÒƱ) <Ů«-îų*Ħã+Ć:%+( 6**ƷZdNPms2!Ë£@ µ$ &+Ʒƭƛ*rî)¤2=$ <5ƷŮ*ĦãĚƐŀŠ 7)Æ¥$ &@ŋ¸$ <îų+à)*rî) <5Ʒ ĦñƔ)ŷ. ±)6Ů0')ñƔ(Ħãp@ŜƱ$ ( *%+(. &vô$ <=:*&:ƷZdNPm*Ů) $N_hBL lHÆ¥(!*+ƷN_hBLlHÆ¥*5)ƝŽ(ĦãÀ@ŚĄ Ɠ;Ɩ$2!$ <:%+( &ŧ !$ƷCHA @ķ ÒƱ%+Ʒîų&Ů%ĺ(<ŝĐí:=6**ƷƙúŀN_hBLlH *ľIJÆ¥<Ž»+ƷŮ&îų) $²Ė% ;ƷƏã(Ħ% < ®ŬòƲ &ŧ :=< . .
(76) %ƷƏã(Ħ=ŴN_hBLlH@Æ¥<®Ŭò@ēƅ ´ƳƜŹ% <BO^jhl@ķ $ƷĦƈŖrđ@ƳƜ<ĂĠ )9;Çƛ:ZdNPm*@©ƷĦ@ 28% 2 ćƠşû* ĂĠ)9;ƷƏã(ĦãÀ@§)ŜƱZdNPm@N_hB LlH@Ɓď&>Ʒshort form )Ú$ long form Ć:)ĥÜ< ńĖ*ƙúŀN_hBLlH*\Pml)Æ¥$ *ľIJ\Pml +ƷĦ 102%p±)6şû=2ƷBO^jhl@ķ ĂĠ% !$6ƷƏã(ĦãÀ@ŚĄƓ;Ɩ<9
(77) )Ĕã*Ħ2% ñ±)+ƷşûćƠ@åƟ&$6Ʒîų«-Ů%* CIRP *N _hBLlHÆ¥(!!$ƷBO^jhl@ķ ĦƇ Û)9!$ƷN_hBLlHÆ¥)Ú<Əã(ĦãÀ*ƝŽòèŋ¸ =CHA @ķ ĦƇÛĠ%+ŚĄĦp±)+Ʒîų) $N_hBLlHÆ¥CHA @ķ ĦƇÛĠ%+Ħ 32zƒ)2%p$:Ÿä)ōƷÇƛ:©$ <* 5Ʒ28zƒ@ƓƖ<5)+ř 60 Ŏã@ŽnĂƷBO^jhl )9<ĦƇÛĠ%+ƳƜłì:Ÿä)$©@Ż!$ <* 5Ʒ28.Ɨ<5)!ćƠ+ 30 ĊĨ% !!$Ʒ CHA @ķ <ĂĠ&BO^jhl@ķ <ĂĠ%+ƷƏã(ĦãÀ%*νć Ơ)Ƙ !5)N_hBLlH\Pml)6Ƙ ž=&ŧ :=< Ŵľŀ(ń) $Ʒƭ@*)¤3Ƒ49
(78) )@s5Ë£@& ;Ʒ8!;&Ħ@p$ ĖÌƀÙ=<Ƶ19, 31ƶ! $ƷŮ@³5tŽ(ų¹+ñī)7=$ ( &ŧ :=<ČÒƱ) $ƷƏã(Ħ)9;Æ¥ƙúŀN_hBLlH+ƷĔã*Ħ .ƇÛì)6ńĖ)Æ¥22% !*&:ƷŴľŀ( . .
(79) ń) $6ƷƏã(Ħ@ƟćƠŜƱ<&)9;Ʒ*Ơ) CIRP * ƙúŀN_hBLlH@Æ¥Ʒ*ì*Ĕã*Ħć)ƍ<ƤÔ) $ <®Ŭò < Əã(Ħã% CIRP ƚ~Ì*ƙúŀN_hBLlHÆ¥<*% =,Ʒ áàć:Ħ):=$ <ŗÝ) $+Ʒńć%(&6N_hB LlH\Pmlńć&²Ė% <®Ŭò <bCN%*Á¶Ƶ46ƶ& ²Ė)ƷLiAlZdNPm*ŗÝ*Ħã+Ʒáàć:ŲŰ9;6Ħ) şû=$ *9
(80) )ĦĎ}p) <ŗÝ)< CIRP ƚ~Ì*ľ IJ@ƈ/&>ƷŒ 1 ő%Ɓď@Ż!ŮƷîųƷūƷũų«-ůų&+ĺ (;Ʒáàć:ńĖ*N_hBLlH\Pml@ŋ$ <&Ć: &(!²n% !$6ų¹)9!$N_hBLlH*\Pmlĺ( !$ &:ƷńĖ* CIRP *ƙúŀN_hBLlH*ƈŖ)&!$Ʒ Əã(Ħ*6*ƝŽ(Ž»% <&@ŋ$ < :)Ʒ]T7bCN*ŗÝ%+ CIRP Pl\GƌĖ ( RNA *ÏÑò@ Ʋ5<ęŬ@ľýƷŗÌéö)Öq$ <Ƶ34, 59ƶĸŗÝ7ŗÝƧŭ ļœ*Ļó%+ŗÝĦã 33zƒ9;6oą<=:*Ļó%ŗÌéö )ĺà@*+ƷCIRP ƚ~Ì*ľIJp<5% <&ijƁ=$ <Ƶ34ƶLiAlZdNPm) $6ŗÝĦã+=$;Ʒá àć: CIRP Pl\GƌŗÝ) $ęŬ$ <®Ŭò+§)ŧ : =<"2;ƷCIRP *ƙúŀN_hBLlH*\PmlńĖ% <& ƷCIRP ęŬ<5)ƝŽ% <®Ŭòŋ¸=à)Ħ% < ŗÝ) $ƷƙúŀN_hBLlH*ƈŖęĕͽ<*'
(81) +ÉÆ Ŷ·Ĥ İ)ƷĸŗÝ7ŗÝƧŭļ*9
(82) (Ļó) $ƷČʼnŏ%ē = long form *ľIJƲ2!$ <(:,Ʒlong form *ľIJ7ęŬ@÷ <&ƷŗÝ)<Ļó*ā(ĞĽĠ)Ţ<®Ŭò < . .
(83) Œ 2 ő%+Ʒ$*ÒƱ) $ƷƏã*Ħ&
(84) Ď}p%+ CIRP *ƙ úŀN_hBLlHńĖ% <&@ŋ!$ƷCIRP *ƙú ŀN_hBLlH@ńĖ)Æ¥<t(Ž»+Əã(Ħ% <&ŝƉ# LiAlZdNPm+ƷŴľŀ(ńć) $ƷńÛć*8! ;&Ħp*Ơ)Əã(Ħ@ƟćƠŜƱƷCIRP *ľIJĖæ@Æ ¥$ <&ŧ :=<. . .
(85) ı 3į. ƏĨĘ/A CIRP 0ĨąŽêĥSelHRqO0ŨÍ. þĪĭ0ùļĥ.ĦĆ1ƚĨĘ0<( ēʼnåEĨ.Ę<©; $®xĘŗ/}wƚ ē0ĖEĐĢA,+Aı 2 į+1ƚ ĨĘ+ARmFq`jSVrEĢ*ƚŕĤĥ.Ĩ,1ěĮ$òĎ +'*<ƚŴÕ. ēEŃëA,/>'*ƚĨą CIRP 0ŽêĥSe lHRqOEØů,+$0ĩš1ƚĨ.Ę/* <ĨĘ,¨ą0 CIRP žÃĤĞEŨÍ+A¥ŏåEī¯*AƏ ĨĘ/*< CIRP 0ŽêĥSelHRqOEūĴAkK]TjŶ A.?2ƚCIRP 0ĉŏ/>@ûÜBA ēʼnå0āEƚƏĨĘ/} w+A¥ŏåA"+ı 3 į+1ƚĨ.Ę,*hIS¤4 lWZEĢƚCIRP 0ŽêĥSelHRqO0kK]TjĨʵú0< 0.0ƚƏĨĘ+'*<ŶA<0.0-Eö?/A ,EĦĥ,$ RmFq`jSVr/AŽêĥSelHRqOūĴ0ęà1ƚÔÒē ÷/Şð0SelHRqOamFqZŶA,ƚ8$ƚ"B?0Şð0 SelHRqOamFqZƚŴÕ. ē/>'*ƚCIRP EPr[A3, (0 mRNA /ƍķA,+A"+ƚ8. 1ƏĨĘ/*ƚŞð. 0SelHRqOamFqZÔÒ÷/ĤĞ*A0-Ăť$ł *ƚƏĨĘ/*<ŴÕ. ē/>'*ŽêĥSelHRqO0¿ ØůBA0-EĂť$. . .
(86) Ŀā. ÔÒēŇ0hIS/A CIRP mRNA ţĀ ÔÒēŇ0hIS0ŒƚáŔƚōƚŊŔƚœŔ¤4ĵÏ/*ƚCIRP ž ÃĤĞ0ĂĹE RT-PCR Ď/>@Ŝ'$ŒƚáŔƚōƚŊŔ¤4œŔ? 1 1 Ľ0elHhr?ķ 500 bpƚ700 bpƚ800 bp 0 3 (0QHT0 PCR ġĘ ¾ÓB$Ƙ´ 21ƙRmFq`jSVrEĢ$ÈƖ,¨ą/ƚķ 500 bp 0ġĘE short formƚķ 800 bp 0ġĘE long form ,$8$ƚĵÏ?1ķ 500 bp 0 short form ĂBƚ|0 2 (0QHT0 PCR ġĘ1 short form ,ċ ŵ*ö?/Ś,*ĂB$Ƙ´ 21ƙ áŔ?¾ÓB$ PCR ġĘEĢ*ƚRrNJqSţĀ/>@¼¹ƁE ţŪ$Ŀā,*ƚshort form 0Ɓ1ƚYrVfrS/º¬0AhIS 0 CIRP mRNAƘNM_007705.3ƙ,Ç/sŖ*$Ƙ´ 22ƙ8$ƚlong form 0Ɓ1ƚYrVfrS/º¬0AųamFqZƘXM_006513165.1ƙ,s Ŗ*$yçBA ORF 1ƚshort form , ORF 0ƇÂĖ? 502 bp 8+1 Ç/sŖ*$sòƚ3’ ýİ0Ɓ1ģ.'*$Long form 0Ɓ 1ƚshort form 0ùÝ0JMUq0 5’ ýİ/SZWeP[qE©: 217 bp 0 ¼¹ƁìB$<0+'$Ƙ´ 22ƙ. hIS/AŴÕ. ē/>A CIRP mRNA 0SelHRqOĤĞ5 0ÛƑ ()*ƚ`jSVr+ CIRP 0ŽêĥSelHRqOE¿ !$ŴÕ. ēhIS/*< CIRP žÃEūĴA¥ŏåEĂť$ĠÅ0$; /ƄŠ.Ŕ°,*ƚŒ,áŔ/ŀ'*ţĀEŜ'$ÔÒēE«*$ hISEƚƗƂ,£0Ľ9§D!+ēE 28 5ŨÍƚ1 ÷ƈŃë$. . .
(87) RTPCR 0ĿāƚēE 28 +Ńë$Ň+1ƚáŔ¤4Œ/*ƚSel HRqOamFqZ short form 5ƍķ$ƚĨŇ,Ɠ0bVrqĂ B$Ƙ´ 23ƙ 8$ƚhIS0ēEŴÕ. ē¸/Ĕ¶!. ƚäŷ/ 15 8+ u!. $»§/1 CIRP 0SelHRqO/ÛƑEwA-EĂť$hIS /HUcnlqEª!$Ýƚŷ=/ 4 /Ńë$řÖ/Ĭ!$ 0òĎ/>@ƚ32-28 0ēÕ¸/Ĕ¶A÷ƈ1 5-7 %'$67-72 Ƙ69
(88) 2 , n=3ƙÝ/hIS0đƀē 15 /Ż$CIRP 0SelHRqOb VrqEţĀ$,CƚēE 15 8+ä$Ň+1ƚáŔ¤4Œ/ *ƚshort form 50ƍķ1ö?/ŧ;?B.'$Ƙ´ 24ƙ ?/ƚHUcnlqEªÝƚēE 37 /Ńë$Ň+1ƚáŔ¤4Œ /*ƚŽêĥSelHRqO0bVrq0¿ 1ØůB.'$Ƙ´ 25ƙ 8$ƚŒ¤4áŔ/*ƚshort form , long form 0ĤĞƅ0ċEijƚ ÔÒēŇ0`jSVr,ċŵ$,CƚēE 28 +Ńë$Ň+1úè .Ñŧ;?B$Ƙ´ 26ƙsòƚēE 15 8+ä$Ҥ4ēE 37 /Ńë$Ň+1ÔÒēŇ,ċŵƚŁŤÆĥ/úè/¿ *A,1 ŧ;?B.'$Ƙ´ 26ƙ. h IS/AÊğ½+0ƔŌ/>A CIRP mRNA 0SelHRqO ĤĞ50ÛƑ ŝƐ?0Êĕ/>@ CIRP 0ŽêĥSelHRqOūĴ¿ A 0-EĂť$"0$; 4 0ğ½+ 1 ŹƈƔŌ$Êğ½ƔŌŇ0 hISE CIRP mRNA 0ţĀEŜ'$RT-PCR 0ĿāƚhIS0Œ¤4 áŔ? 3 þ0aq[ĂB$Ƙ´ 27ƙ8$ƚshort form , long form 0Ĥ. . .
(89) Ğƅ0ċEij$Êğ½ƔŌŇ,ÔÒēŇ0hIS/*ƚúè. Ñ1ŧ;?B.'$Ƙ´ 28ƙ. Ô ÒēŇ0lWZ/A CIRP mRNA ţĀ ÔÒēŇ0lWZ0ŒƚáŔƚōƚŊŔƚœŔ¤4ĵÏ/*ƚCIRP ž ÃĤĞ0ĂĹE RT-PCR Ď/>@Ŝ'$áŔƚōƚŊŔ¤4œŔ?1 1 Ľ0elHhr?ķ 500 bpƚ700 bpƚ800 bp 0 3 (0QHT0 PCR ġƾ ÓB$Ƙ´ 29ƙRmFq`jSVrEĢ$ÈƖ,¨ą/ƚķ 500 bp 0ġ ĘE short formƚķ 800 bp 0ġĘE long form ,$8$ƚĵÏ?1ķ 500 bp 0 short form ĂBƚ|0 2 (0QHT0 PCR ġĘ1 short form ,ċŵ* ö?/Ś,*ĂB$Ƙ´ 29ƙ?/ƚŒ?1ƚķ 1.1kbp 0 PCR ġĘĂB$8$ƚŒ/*ķ 500 bp 0 short form 1¢/ĂB $<00ƚķ 700 bp ¤4ķ 800 bp 0 PCR ġĘ1 short form ,ċŵ*ö?/ Ś,*ĂB$Ƙ´ 29ƙ. lWZ/AŴÕ. ē/>A CIRP mRNA 0SelHRqOĤĞ5 0ÛƑ ()*ƚ`jSVr¤4hIS+ CIRP 0ŽêĥSelHRqOE¿ ! $ŴÕ. ēlWZ/*< CIRP žÃEūĴA¥ŏåEĂť$ 22 +ƔŌ*$lWZEƚƗƂ,£0Ľ9§D!+zėĥ/ 28 0 ē+ 1 ÷ƈŃë$RTPCR 0ĿāƚēE 28 +Ńë$Ň+1ƚáŔ¤ 4Œ/*ƚSelHRqOamFqZ short form 5ƍķ$ƚĨ÷,Ɠ 0bVrqĂB$Ƙ´ 30ƙ. . .
(90) ´ 21ƜÔÒēŇ0hIS/A CIRP mRNA ĤĞţĀ. ÔÒēŇ0hIS/*ŒƚáŔƚōƚŊŔƚœŔ¤4ĵÏ/ĤĞ* $ CIRP mRNA 0 RT-PCR ġĘ0ƎČďEīPCR 1* 30 QHNn+ Ŝ'$ħĈ0ð1hrKr0 bp Eīķ 500 bp 0ġĘE short formƚ ķ 800 bp 0ġĘE long form ,$~ŝ,* 3 ¡0hIS/AĿāEī . .
(91) ´ 22ƜhIS/A short form ¤4 long form 0JMUqĄŸ. Short form ¤4 long form 0JMUqĄŸEć×´+ī1ƇÂP[qƚ 1ļĊP[qEīðÄ1ƇÂP[q?0¼¹ðEīOor0±Ţ+ ī$ƀƚlong form 0& short form ,ģ.AƁ+A. .
(92) ´ 23ƜēE 28 +Ńë$Ň0hIS/A CIRP mRNA ĤĞ. ēE 28 +Ńë$hIS/*Œ¤4áŔ/ĤĞ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČďEīPCR 1* 30 QHNn+Ŝ'$ħĈ 0ð1hrKr0 bp Eī~ŝ,* 3 ¡hIS/AĿāEīÐ /ċŵÌŭ,*ÔÒēŇ0 PCR Eī. .
(93) ´ 24ƜēE 15 8+ä$Ň0hIS/A CIRP mRNA ĤĞţĀ. ēE 15 8+ä$Ň0hIS/*Œ¤4áŔ/ĤĞ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČďEīPCR 1* 30 QHNn+Ŝ'$ ħĈ0ð1hrKr0 bp Eī~ŝ,* 3 ¡0ĿāEīÐ/ċŵ Ìŭ,*ÔÒēŇ0 PCR Eī. .
(94) ´ 25ƜHUcnlqEªÝƚēE 37 /Ńë$Ň0hIS/A CIRP mRNA ĤĞ. HUcnlqEªÝƚēE 37 /Ńë$hIS/AŒ¤4áŔ/Ĥ Ğ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČďEīPCR 1* 30 Q HNn+Ŝ'$ħĈ0ð1hrKr0 bp Eī~ŝ,* 3 ¡0Ŀā Eī ċŵÌŭ,*ÔÒēŇ,ēE 28 +Ńë$Ň0 PCR Eī. .
(95) ´ 26ƜhIS/AHUcnlqƗƂ/>A CIRP SelHRqOamFqZ0¿ . ¦0Œ¤4áŔ/A short form , long form 0ĤĞƅ0ċƘlong form/short formƙEij$ÔÒēŇƘn=5ƙƚēE 28 +Ńë$ŇƘn=4ƙƚ ēE 15 8+ä$ŇƘn=3ƙ¤4HUcnlqEªÝƚēE 37 / Ńë$ŇƘn=4ƙEÔ·
(96) SD +īÁƄċŵ/1 Dunnett’s ĎEĢƚ ÔÒēŇ,"B#B0Ň0Eċŵ$**1ÔÒēŇ,ċŵ*ŁŤĥ /úè+'$,EŝƘP < 0.01ƙ. .
(97) ´ 27ƜÊğ½ƔŌŇ0hIS/A CIRP mRNA ĤĞţĀ. Êğ½ƔŌŇ0hIS/*ƚŒ¤4áŔ/ĤĞ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČďEīPCR 1* 30 QHNn+Ŝ'$ħĈ0 ð1hrKr0 bp Eī~ŝ,* 3 ¡0ĿāEīċŵÌŭ,*Ô ÒēŇ,ēE 28 +Ńë$Ň0 PCR Eī. .
(98) ´ 28ƜÊğ½ƔŌŇ0hIS/A CIRP SelHRqOamFqZ0¿ . ¦0Œ¤4áŔ/A short form , long form 0ĤĞƅ0ċƘlong form/short formƙEij$ÔÒēŇƘn=5ƙ ƚ ēŇƘn=4ƙ¤4Êğ ½ƔŌŇƘn=5ƙEÔ·
(99) SD +īÁƄċŵ/1 Dunnett’s ĎEĢƚÔ ÒēŇ,"B#B0Ň0Eċŵ$**1ÔÒēŇ,ċŵ*ŁŤĥ/ úè+'$,EŝƘP < 0.01ƙ . .
(100) ´ 29ƜÔÒēŇ0lWZ/A CIRP mRNA ĤĞ. ÔÒēŇ0lWZ0ŒƚáŔƚōƚŊŔƚœŔ¤4ĵÏ/ĤĞ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČďEīPCR 1* 30 QHNn+Ŝ'$ 2 ¡0lWZ/AĿāEīħĈ0ð1hrKr0 bp Eīķ 500 bp 0ġĘE short formƚķ 800 bp 0ġĘE long form ,$. .
(101) ´ 30ƜēE 28 +Ńë$Ň0lWZ/A CIRP mRNA ĤĞţĀ. ēE 28 +Ńë$lWZ/*ƚŒ¤4áŔ/ĤĞ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČďEīPCR 1* 30 QHNn+Ŝ'$ħĈ 0ð1hrKr0 bp Eī~ŝ,* 3 ¡0ĿāEīċŵÌŭ,* ÔÒēŇ0 PCR Eī. .
(102) ňË. ı 3 į+1ƚĨĘ+ARmFq`jSVr/*Ŷ$ CIRP ž Ã0ŽêĥSelHRqO0ūĴkK]TjƏĨĘ/<ŶA0Ăť$"0ĿāƚƏĨĘ+AhIS¤4lWZ/*<ÔÒ ē÷/1ĉŏĥ. CIRP mRNA E©:Şð0SelHRqOamFqZÅ ¶A,ö?,.'$8$ƚ`jSVr,¨ą/ƚŴÕ. ēƚ CIRP 0ŽêĥSelHRqO0bVrqE¿ !A,<īB$ þĪĭ/*ƚhIS¤4lWZ/*<ƚÔÒ÷? CIRP mRNA E© :Şð0SelHRqOamFqZ0ĤĞŧ;?B$0,?ƚhI S¤4lWZ+<ƚĨĘ,¨ą/ƚŽêĥSelHRqOE@øA0 9+ĉŏĥ. CIRP mRNA 0ĤĞ0ĝE¾=,+A¥ŏåAh IS/A long form 0Ɓ1ƚYrVfrS0<0,sŖ*$<00ü %ĉŏ/(*1º¬B*.<0+'$Ɓ?yçBA long form 0Pr[AVqbNŮ0ĄŸ1ƚĉŏĥ. CIRP 0 C ýİ0 5 (0Fi _ƃƚģ.A 9 (0Fi_ƃƁ/ņíD'$Ú+'$0Ɓ0Ą Ÿ1ƚı 1 į+ö?,.'$RmFq`jSVr0 long form 0ĄŸ,¨ą+ A$'*ƚÔÒē÷0RmFq`jSVr,¨ą/ƚƏĨĘ+ AhIS¤4lWZ/*<ƚlong form Pr[AVqbNŮ1ĉŏĥ . CIRP VqbNŮ0[i\qZ^LXGd,*0ĉŏEë(¥ŏåA (8@ƚŽêĥSelHRqOE@øA,/>@ƚCIRP VqbNŮ0Ĥ ĞĝEtõ!A%+.ƚĉŏE¢/Ĥî!AkK]TjhIS ¤4lWZ/*<D'*A,ī¯B$ ı 3 į+ù<ƄŠ.Ŀā1ƚĨ.hIS¤4lWZ/*<ƚzė ĥ/ ē/ŨÍA,/>'*ƚĨą0SelHRqObVrq50¿. . .
(103) EĞ+$,+Azėĥ. ēŨÍ1ƗƂśEĢ*ŜDB$ ƚƗƂśEª!$Ý/ 37 +Ńë$»§/1SelHRqO1¿ .'$$;ƚSelHRqObVrq0¿ 1ƗƂś0ÛƑ+1.ƚ ē0 u/ÅA<0,A8$ƚhIS/*1ƚäĕ/ēE u!$»§/1 CIRP 0SelHRqObVrq1¿ .'$$;ƚŴ Õ. ēŽêĥSelHRqOE¿ !A$;/ƄŠ+A,EÙ ïëAĿā,.'$ŽêĥSelHRqOŴÕ.ēÕ¸+ØůB A,1ƚRmFq`jSVr,¨ą+A$'*ƚ ēEĕ,* CIRP EūĴAkK]TjĨʵú0<0+1.ƚƏĨĘ/< Ŷ*Ŷ*A¥ŏåA,ī¯B$0ň,sŖ*ƚh IS+1ƕ0vŰ,ğ½ēÕ0 u/>'*ƚ1 ó+ēE 10 6- u !AóĨEŜE,A,ĩ?B*AƘ3ƙ8$ƚlWZ/ *<ƕ0vŰ/>@ƚ1 +1A<00đƀēE u!AĉĄ D'*AƘ42ƙ$'*ƚŲ+ē uAĚč1ƚĨ0>.ăÕ 0 ē+.*<ŶA"0$;ƚĨ.Ę/*<ƚ ē/ >A CIRP 0ĤĞĉĄÅB*A¥ŏåA ŝƐ?0 ēĕŽêĥSelHRqOE¿ !A-EĂť A$;ƚhISEÊğ½u/* 1 ŹƈƔŌ$1 Źƈ0 ēğ½ƔŌ, ÿ1ƚhIS/*şŘŐŋĽŅ0źÚé/~ŝBAÊ50ƒ ŧ;?BAÿ+AƘ47ƙ(8@ƚ0ÿ+1ƚŝƐ?0 ēĕ1 ¢/Ų/ÛƑE¤7*A,ň?BAÊğ½ƔŌ09+1ƚ ŽêĥSelHRqO0bVrq/úè.¿ 1.'$$'*ƚCIRP 0SelHRqObVrq1ŝƐ? ēĕ+1.ƚĽŅ"0<0 ē,.AâŠA,ö?,.'$ÈƋ/ƚĽŅ0ēÕđƀē>@ ĵÏ+1ƚÔÒē÷/*< long form Śƚshort form ÙĤĞ. . .
(104) *$"0$;ƚŴÕ. ē"0<0ŽêĥSelHRqO/ÛƑA, ũEïëAĿā,.'$ lWZ0Œ+1ƚlong form 1ƚÔÒē÷/<ĤĞÎ.<00ƚŴÕ. ē/>@ long form 0ĤĞ1>@ĒÎ*A,<ö?,.'$$ '*ƚŒ/*<áŔ,¨ą0ŽêĥSelHRqO0ūĴ/>A CIRP ž ÃßĉĄ1ŶA,ň?BAsòƚlWZ0Œ?1RmFq`j SVr=hIS?1ĂB.'$ķ 1.1kbp 0aq[ĂB$8$ƚ ŴÕ. ē/*<ķ 1.1kbp 0aq[1¿ E.'${Ýƚķ 1.1kbp 0 PCR ġĘ0ƁEţĀAâŠA<00ƚlWZ0Œ/Ɖ*1ƚRm Fq`jSVr=hIS,1ģ.AkK]TjŶA¥ŏåA ıƛį+1ĨĘ/*ƚCIRP žÃ0ĤĞūĴƚ ē/ƊAƋ/ ĸô CIRP VqbNŮ0ĉŏEĤîA$;/ƄŠ+A,ňË*$s òƚþį/*ƚĨ.Ę+AhIS¤4lWZ/*<¨ą0 ŽêĥSelHRqOĤĞūĴŶA,ŧ;?B$0,?ƚ B?0Ę+1Ĩ÷0>. ēƌÉ?ŬAĦĥ,1ģ.AĦĥ 0$;/ŽêĥSelHRqOūĴEĉŏ!*A¥ŏåACIRP E_ WNFIZ$ĻŎ+1PqZprn0ĻŎ,ċŵƚmitogen-activated protein kinaseƘMAPKƙ= WntƚFgZrRSIJ0RO\nľű0ĤĞ/ö?.ż šB*A0,?ƚCIRP 1æÒĥ/ĉŏ*A,AƘ51ƙ B?0RO\nŻĶ1ĻŎ0ĕ/Ì*ĸôÌãƚĠ0æÒåE '*A(8@ƚĨ÷0>.Ų ē/ƊA,Ěču+. ,<ƚĻŎofn+1ƚŽêĥSelHRqOEĢ$ CIRP 0ĤĞtõĉĄƚ Ġ0æÒåEŃëA$;/ĉŏ*A¥ŏåA8$ƚCIRP Vqb NŮ0ĤĞ0tõ/1ƚ ē0|/< ƃĸƚĺÀńIJ0ĕØƆ,.A Ƙ45, 55, 64, 65ƙ"0$;ƚ ēÀ0г/>'*ƚCIRP 0ŽêĥSel. . .
(105) HRqOūĴØůBƚæÒåE($;/ĉŏA¥ŏå<A{ ÝƚCIRP 0ŽêĥSelHRqO0 ēÀ0г,ā/(*<ö?/ *,ûÜBA þĪĭ+1ƚRmFq`jSVr1ƚCIRP 0SelHRqOß/>@ ē/ÌAʼnåEĜÞ+*A¥ŏåA,ň*Aþį0Īĭ/> '*ƚĨ.Ę+AhIS,lWZ/*< CIRP 0ŽêĥSelH RqOEĨą/ß+A,ö?,.'$$'*ƚƏĨĘ +'*< ē/ÌAʼnåEĜÞ+A¥ŏåī¯B$Ĩ. ĘEÙĥ/ ē,A,/>@ƚę/Ŗĥ.ƌÉ1ƚáŔ/A vñő+AƘ13ƙ"+ƚĨą0 CIRP ĤĞą×088 ē/ÍA ,/>@ƚáŔ/ůA ē÷0ƌÉE²ſA,ûÜ+A{Ýƚ ƏĨĘ0 ē÷0ƌÉ/Ì*-0>.āEĤîA0-Ŧ Ļ/ţĀ*âŠA. . .
(106) š ¡ŦÈ. ďŇŏ&ƸŃĮ%0{|Īŧê8ƬŃĮ$uj5! $25ׯ8Ɨ5!8ĉŝŀ#łĚ!5. {|Ī. Ƹ{Īi. Ŀĵ. 8hĄƸ3$ŜŨ%Ƈěũ8Ċ5{ĪD_JAIeSAƊ CIRP $ ńłŔ 1 ő. &ŃĮ. 5Da:eQ\FIf$ 5ŃĆ%. CIRP %ƕóŀFW`<DeB%¸8ą3 !Ŕ 2 ő W`<DeBƂŕ%¸8Ù
(107) ƋŶ® ƍÖ%{|Ī ŹąŔ 3 ő. &%ƕóŀF. 5!$. &ƬŃĮ$ Ńę% CIRP %ƕóŀFW`<D. eB%Ƃŕ8ĵ5!$ð%!$24ƸŃę% CIRP %ƕ óŀFW`<DeB%Ƃŕ8sƸƬŃĮ$0{|Īŧê8uj.
(108) 5. ũê ʼn©6ƶ° 31Ʒ ĕĮ$ &ƸĪÖ$24ƕóŀFW`<DeB ¸5!. %´¥ 4ƶ37, 53ƷƸ%]?OG\ ¨mĮ$0 Ɛ5 ũê 53$Ƹ¨mĮ%³ƲŜŨ8ĺŇŏ$2Ƹ{γƲ$24 CIRP %Ŀĵ%hĄ!!0$ƸƕóŀFW`<DeB$ƞŶ#®¾. 5 SRSF5. %Ŀĵ hĄ5!´¥65ƶ15Ʒ Ƹĸ|. 0 SRSF5 %. ¸8s CIRP %ƕóŀFW`<DeB ¸ ũê 5!Ŧ 5,4ƸƍÖ#{Ī$24ƕóŀFW`<DeB ¸5!Ŀŷ &Ƹ¨mĮ$ 5ƕóŀFW`<DeB8sĪÖ$25IeSAƊĿ ĵ%ƂŕěĘ8ĸ| 6,. Ƹ{Īi. lŶ#Ŷ®. ŷƞŶ#Ņŷ. 5. % CIRP IeSAƊ%hĄ$&ƸƌcXb%Ħê. 5!Ŧ36
(109) Z=F%ŢŠůŜŨ8ĺŇŏ$ . ƸƍÖ#{Ī&ƕóŀWd^fIf8¸5!$24Ɩy¾%ƌ8 Ħê5!´¥ 5ƶ1Ʒ,Ƹƌ®¾ Sp1 32%ĪÖĐxi. 73.
(110) $ ĦêƸCIRP mRNA %hĄ$Çj5ƶ49ƷgāƸƌà Ƃŕ$25ěũŀ# CIRP %hĄ$0´¥ 5!Ƹ{Ī&Z =F%ŢŠůŜŨ$ & CIRP % pre-mRNA 3%FW`<DeBĴ8 hĄ5ƶ17ƷďŇŏ. ą3 !ƕóŀFW`<DeB%¸&Ƹ¨. mĮ% CIRP %Ŀĵ]?OG\$ ĀŸ%Ŀŷ!# ŃĮ8ĺƸŃĆ%¸$ą3 $5!8łŀ! %Ňŏ Ŵ765%k%(!$ƸDZaF8ĺ Hibernation proteinƶHPƷ%Ňŏ 5ƶ24ƷDZaF hĄƸūŋŞř. ƞŶ#Ý8ô5%. &ƸŃĆ$%-ū. HP . &# !´¥65. 63%Ňŏ$ĺ365DZaF1ƸEaF#"%ŃĮ&Àŕê% Ń8Ŵ Ƹ63%Į8ĺá36¸$&Àŕê%® ¾ ƣj5 ũê8Ŧî5æŶ 5ÄƩ$ƸHP %Ŀĵ$&Ô¦ aG\ƶcircannual rhythmƷ ƣj5ƶ24ƷgāƸďŇŏ Da:eQ\FIf&ƸÄƳÅ. ÄƳ$ĺ. {Ī)ąĈķ¶8ĈČ ơ#52. $Ƃŕ5!$24 1 Ô8ƐŭĿŀ#Ń8Ŵ!.
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