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シリアンハムスターにおける低温ショックタンパク質の発現調節に関する研究

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(1)Title. シリアンハムスターにおける低温ショックタンパク質の発 現調節に関する研究( 本文(Fulltext) ). Author(s). 堀井, 有希. Report No.(Doctoral Degree). 博士(獣医学) 甲第541号. Issue Date. 2019-09-20. Type. 博士論文. Version. ETD. URL. http://hdl.handle.net/20.500.12099/79040. ※この資料の著作権は、各資料の著者・学協会・出版社等に帰属します。.

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(7) Ǘ ɒ˜Ɂ. aCSF. artificial cerebrospinal fluid ¢ķȦȥʝƸ. CHA. N6-cyclohexyladenosine. CIRP. cold-inducible RNA-binding protein. HP ORF.  . Hibernation protein open reading frame. RBM3. RNA-binding motif 3. RNP. ribonucleoprotein. RT-PCR. Reverse transcription polymerase chain reaction. SD. Standard deviation ƛƿ»ĸ. . .

(8) ȏɆ.  |sOôDĚ9ýŸÞLj:°ƽŗĹŖOŧ/ʥęɴƽĿĘà%3Fƹɴ °ƽO˜Ħ8¸03K-EʥĤĦ%-ƴÞOȍŧ'K"54K˜Źʥ y‡gl—Ggʥ‹ w769˜ɴ9IJċ!0Ơʖ:ʥ°ƽŗĹŖOŧ03 K8FNI(ʥʙ›ɞ%ƪƽ¯š'KǏĕš83ʥȬI°ƽO ǏĕƽĿ8ɤ’‘8B4š!K ʣ39ʤ"9ǍɘOÉǥ5ÉǥÞLj :ʥÉǥOȶ"58H03°ƽOȍŧ'K-E9Ww‘]—ƷəOŠ3 K5ǎɄ#L3KÉǥOȶN7Ě9ýŸÞLj:ʥ±I9æĄ8HJ ƹɴ°ƽ¯š%-Ēò8:ʥőŦÞOȍŧ'K"54(8ơ8ȭK´ ;ʥ|s4:°ƽ 27OšăK5ʥŋÞǟ7őŦÞOȍŧ'K"54 (ʥĚ9Ēòő¼ƞOɝ"'"5ǧIL3Kʣ30ʤ˜ŹʥÉǥÞLj:ʥ ÉǥƁʥ°ƽ 10¨š8ɮ'K8FNI(ʥőŦÞGùõ50-ǐú ƴÞOȍŧ'K"54K"9"5IʥÉǥÞLj:¯°ƽ8ȚKljƢ 7ƝƙO¸ƈ%3K5șILK  ˜ȯǟ8ʥ¯ƽƁ8:ʥȆȢÆ9ƚ7ɸȃ9ƴŖ¯š%ʥ§ɖƝȣ¯ š'K"9"58HJâǜÎɻ83:ƚ7ˆosƉŊ4KĨ ʉ8¯°ƽ:ʥőǸƔēʥȥʝů¾ʥȦƔēGȦę¾ʥȫĀǯƕʥƷàǺȳȵ 76I9 ŌOűÿ'K"5đ÷#L3Jʥ+9ƈÝŖɍſ#L3 Kʣ10, 20, 43, 48ʤB-ʥPGśƓǛ769ɬȶOŠÖ'K98F¯°ƽ ƈÝ4K5șIL3Kʣ57, 58ʤɺǼ7ʊĪ9ƣKîȣŖ9ʞǚŘ 4KŸǐÂȦǛ83Fʥ¯ɹȃ8HJńɝ"#LKȦʊĪOȑû'K -EȦB-:ÄɟOÊå'K¯°ƽǜƮưǡ#L11Kʣ16, 44ʤ%% 7Iʥ|sOôDÉǥOȶN7Ě9ýŸÞLj:ƗĿ8°ƽ¯š'K5 ǐúƴÞOȍŧ47%-03ʥ¯°ƽ9ˆosOãÎ8âǜŔǒ' . .

(9) K8ȭ037+"4ʥÉǥÞLj9ŧ1¯°ƽȚŖ9ˆZvh‡OɄſ%ʥ |sOôE-ʐÉǥÞLj8ɰǒ'K"54L;ʥ¯°ƽ9ˆosOâǜ Ŕǒ4K5ƉŊ%-  ƌǩDz4:ʥÉǥÞLj8. K¯°ƽȚŖ9ˆZvh‡OɄſ'Kǿí5%. 3ʥ¯ƽeŽo^l”{^ɛ8Ǧǡ%-ól”{^ɛ:¯ƽ769ȆȢgs ’g8H03ɑı#LKl”{^ɛ~Q†—4Kʣ28, 63, 64ʤ¯ƽeŽ o^l”{^ɛ5%3óĦ#Lʥƙɩ7I=8Ɲȣ9ɄƑɬP4KF95 %3ʥCold-inducible RNA-binding proteinʣCIRPʤ5 RNA-binding motif3ʣRBM3ʤ ũ!ILK"LI9l”{^ɛ:ʥN ƋǶ½8 RNA ȊòtˆT”OʥC Ƌ Ƕ½8R‘]v”_e”ontˆT”Oŧ1B-ʥCIRP é= RBM3 :ʥ |sʣ6, 35ʤG Ugʣ7, 36ʤʥosʣ9, 60ʤ76Ě9ÞLjDZ83 ƚ7ȫĀ8ǝǍ%3K"5đ÷#L3K#I8ưǡ'@"58ʥ IJċ!0Ơʖ4Kfgʣ56, 61ʤʥěċýŸÞLj4K^ ʣ11, 12ʤ5 0-Éǥ'KýŸÞLj8Ǧǡ%ʥȎȕǟ7ɲ¬ĠɄƑOȶ0-ǩDz83ʥ Ȧʥőȫʥȝȫé=Ǹț49Éǥ9 RBM3 9ǝǍ9™žđ÷#L3K B-ʥCIRP é= RBM3 : RNA Ȋòl”{^ɛ5%3ʥRNA 9 5’B-: 3’ʐȘ ɌʓčG„Rrv‘àʓč8Ȋò'K"58HJƚ7ɲ¬Ġ9ɠÈŌɕǻ 8ʂœ%3K"5ǧIL3Kʣ29, 65ʤ´;ʥ UgG|s9ȆȢO ǒ-ǩDz4:ʥ3’ʐȘɌʓč8Ȋò%- CIRP é= RBM3 8HJd—ZrSR ”ɲ¬Ġ9„Rrv‘àɕǻ#LKʣ28ʤB-ʥǾĶ4:ʥCIRP ȘɌ ɕǻGŢɹà²ǒ768ʂɪ'K19 mRNA 8Ȋò%ʥĤĦŖOʞE3 Kʣ58ʤRBM3 : miRNA 8Ȋò'K"58HJʥȎȕǟ7l”{^ɛ9Ș Ɍ8ňʑOœ3K"5đ÷#L3Kʣ9ʤ#I8ʥ"LI9l”{^ ɛ8:ʥTNF- GɭɹàƫȃʥŝɾĄĠ9Úĝ768ńɝ"#LKR„s— egOŠÖ'KƝȣK"5FſI5703Kʣ5, 25, 40, 62, 65ʤ¯ƽ . .

(10) 4Ďʘ%- Ug9ȦgTgƛƌ83:ʥRBM3 :ǬȉļȆȢ9ǐĢƉ ʁOłɾ'K5đ÷#L3Kʣ51ʤ"9H8ʥ¯ƽeŽo^l”{^ɛ :¯ƽƁ8ǝǍO™ž#)K"5ʥB-ʥȆȢ¸ɗ݃OF1"5ʥ5> -19ljŐũ!ILK"LI9ljŐ8HJʥól”{^ɛ:ƗĿ9¯°ƽ Ɓ83ʥǐúƴÞOȍŧ'K-E8¸ɗƝȣOǝŮ'KîȣŖK5ș ILKRBM3 813:Éǥ'KýŸÞLj8. KǝǍ9™žđ÷#L. 3KF99ʥCIRP 813:Ɗ.đ÷7+"4ʥƌǩDz4:ʥ¯ƽe Žo^l”{^ɛ9/ CIRP 8Ǧǡ%ʥÉǥÞLj8. KǝǍÞŜOſI8. 'K"5ʥB-ʥʐÉǥÞLj?9ŔǒîȣŖOȾÍ'"5Oǡǟ5%-  ƌǩDz4:ÉǥÞLj5%3ʥeR”y‡gl—Oǒ-ÉǥÞLj8 3ʥȬǝǟ8ÉǥOɑǝ#)K-E8:ʥÉ9H7Ǩżé=ĭÊǏĕš4ʗ ȟ'K"5Œʒ4K"9H7Ǐĕš4ɉà'K"58HJʥÉǥÞLj :ʥȬǝǟ7Éǥ8ŒȽ7ƚ7ɲ¬ĠOĘà#)3KIJċ!0Ơʖ4 KeR”y‡gl—:ʥĨʛĩÆ403FÊȱŁÆ4Ǩżé=ĭÊǏĕO ÇǍ'K"58HJȬǝǟ7ÉǥOɑǝ4KB-ʥÉǥƁ8:ÉǥÞLj9 ȦÆ4Rrxe” A1 ìī°ɚƴà#LK"58HJʥ°ƽ¯š'Kʣ50ʤ eR”y‡gl—:ʥ¢DŽǟ8Rrxe” A1 ìī°RcvgsʣCHAʤOȦ ĩÆšœ'K"58HJʥÉǥƚ9¯°ƽ?ɑı'KŹƮǪǴ#L3Kʣ31, 50ʤ"9H7ljŐOÕǒ%3ʥƌǩDz4:ÉǥÞLj5%3eR”y‡g l—Oǒ-  Ƿ 1 ǵ4:ʥeR”y‡gl—8. K CIRP ɲ¬Ġ9ĻĹƁ5Ȭǝǟ7. ÉǥƁ9ǝǍOɄƑ%ʥǝǍ8ljŐǟ7ĘàĢĈ'K96ƖɈ%- Ƿ 2 ǵ4:ʥÉǥƁ9 CIRP ɲ¬Ġ9ǝǍɕǻOńɝ"'ȽĄOſI8'K -E8ʥÉǥƁ9ʗȟǏĕ5ʥ°ƽ¯š8Ǧǡ%ʥƖɈ%-Ƿ 3 ǵ4:ʥÉ ǥƁ9 CIRP ɲ¬ĠǝǍƚŃ:ʐÉǥÞLj?Ŕǒ4K96OſI8 . .

(11) 'K-E8ʥÉǥ%7ÞLj4K Ug5os8. K CIRP ɲ¬Ġ9ǝǍ. OɄƑ%ʥÉǥÞLj5óƚ8Ęà'K6ƖɈ%-. . .

(12) Ĩ ʛƍŶé=ĨʛŹƮ. ĨʛÞLj  Ĩʛ8:°ɺ 108 g-164 gʥ9-26 ɫʡ9ʋeR”y‡gl—ʥ°ɺ 34-44ʩʥ 8-12 ɫʡ9 ddY Ȁȋ9ʋ Ugé= °ɺ 257-388ʩʥ8-12 ɫʡ9 Sprague-Dawley ʣSDʤȀȋ9ʋosOǒ-'@39ÞLj:żƌ SLCʣʏĵ, żƌʤI ɜÃ%-ĨʛÞLj:¨š9Ĩʛ8µ'KB4ʥĩƽ 22°CʥſƂøƉ 12 Ɓʁʦ 12 ƁʁʣſƉ 7 : 00-19 : 00ʤ8ɋĦ#L-Ǐĕ4ʗȟ%ʥʙ: MFʣYW”l ‘ɸƦ, Ɛ¡, żƌʤOœ-ʙ5ƫ:ȬǓ8Űë4KH8%-  ƌǩDz8. KÞLjĨʛ:Ä3ʥĴʃěģÞLjĨʛğü«4ɐî#L-F9. 4JʥĴʃěģÞLjĨʛëŞȿǰ8ō03Ĩź%-ʣşɐǘð: 15096, 16030, 17015ʤ. ÉǥÞLj9²Í  ƌǩDz4:ʥeR”y‡gl—OĨʛĩÆ4Ȭǝǟ7Éǥ?ɑı%-ǭ IÉ9ǏĕOÇǍ'K-EʥeR”y‡gl—O 4-59ÊȱŁÆ4ʥſƂ øƉ 8 Ɓʁʦ16 ƁʁʣſƉ 18 : 00- 2 : 00ʤ83ʗȟ%-¯ƽǏĕ4:eR ”y‡gl—: 1 á(1¹Ôʗȟ5%-ŭɅ)(8°ƽOƾĦ'K-Eʥq ’ˆs—egq‡ʣDAS-7007; BioMedic Data Systems, rUVR, ǽćʤO ǒ-y‡gl—8:s”g„”m—ʣIPTT-300; BioMedic Data Systemsʤ OȡɴȞǔʜʁ9Ǡš8ČEɣP.°ƽƾĦ9ʉ8:ʥ—m—egq‡ ʣDAS-7007; BioMedic Data SystemsʤOy‡gl—8$'"58HJʥy‡g l—8ŭɅ)(8°ƽOɇƾ%-y‡gl—:ʥ1 •ƇʁĭÊǏĕé=ſƂø Ɖ8ʚà#)3IʥʗŶOÖʅ%-ʗŶOÖʅ%3I: 10 : 00 é= 18 :. . .

(13) 00 8°ƽOƾĦ%-y‡gl—9°ƽ 15¨š57Jʥǡɀ8HKƴÞ¼ ƞʥŭɅ×ǁ8į%39œɳȶÞ9ƷĜé=°OžE-°ßȾIL-Ēòʥ ÉǥOȶ03K5ÓĦ%-Éǥ²ÍƮ:ʥ4 á9y‡gl—OóƁ8ɰǒ% -Éǥ%-y‡gl—:ÉǥȖ5%3Ŭƍ%-"LI:Ƿ 1 ǵ83Ȭǝǟ 7ÉǥƁ9 CIRP ɲ¬ĠǝǍOɕ@K-E9ɄƑ8³ǒ%-4 á9/ 2 B: 3 á9y‡gl—ÉǥOȶ0-Ɓǃ4ʥó&lT†”_4Éǥ²ÍƮOɰ ǒ%3K98FNI(Éǥ%37y‡gl—OʥĭÊé=ǨżǏĕ ʗȟȖ5%3Ŭƍ%-"LI:Ƿ 2 ǵ83ʥ¯°ƽ5:7I7F99Éǥ OȶH7Ǐĕ?ɉà%-"58HK CIRP ɲ¬ĠǝǍ?9ňʑOɕ@KE9ɄƑ8³ǒ%-. R rxe” A1 Rcvgs8HK¢DŽǟ¯°ƽ9²Í  Ƿ 2 ǵ4:ʥÉǥÙ9ĘàOȉ(8Éǥƚ9¯°ƽıÃ9CO¢DŽǟ8ÇǍ %ʥCIRP ɲ¬Ġ9ǝǍ?9ňʑOƖɈ%-+9-EʥÉǥƁ9°ƽ¯š8Ĭ œ%3KRrxe” A1 ìī°9Rcvgs4K CHA Oǒ3¯°ƽ?ɑ ı%-y‡gl—O²Í%-229ǏĕƽĿ83ʗȟ%3JĭÊʚà%3 7y‡gl—8ʥRrxe” A1 Rcvgs N6-cyclohexyladenosineʣCHAʤ ʣSigma, †h—, ǽćʤOȦĩÆšœ%-Ü3ʥęɴIÊå'K"5 8H03ʥ¢DŽǟ8¯°ƽ?ıÃ%-ȦĩÆšœ9-Eʥy‡gl—8:[ TtZvŒ—’ʣAG-6; WTb‡, ¡ɵ, żƌʤOǖȔ%-ǖȔ9ʉ8:ʥy ‡gl—8`l†”ʣ200 mg/kgʤé=\ef”ʣ10 mg/kgʤOƺò%ʥȪȨ Æšœ'K"58HJÄɟʟɷOȶ0-ʟɷ%-y‡gl—ʔɴ9ƩOÐJʥ ȦĆĦȹȔʣSR-6N; Narishige Scientific Laboratory, Ɛ¡, żƌʤ8ĆĦ%-y ‡gl—9ʔȰʜOʎÍ%ʥ’_ 9ŀƛOƾĦ%-y‡gl—9Ȧĉą. . .

(14) ʣ33ʤOèdž%ʥ[TtZvŒ—’Oï½Ȧĩʣ-0.3 mm AP, 2.6 mm L, 4.0 mm DVʤ 8ŪÃ%-ʎÍ%-ʔȰʜ8wfO 2 ƌČEɣCʥ[TtZvŒ—’5wf Oʥr”l‘iˆ”sOǒ3ĆĦ%-[TtZvŒ—’Æ8:m†—Zv Œ—’ʣAD-6; WTb‡ʤOǖȔ%-B-ʥ°ƽOƾĦ'K-Eʥ°ƽƾĦ ǒs”g„”m—OȪȨÆ8ČEɣP.ȷŌʥ¢DŽǟ7¯°ƽ8ɑı'K B4ʥy‡gl—8:ij75F 1 ɫʁ9ăŏƉʁO-CHA :¢ķȦȥ ʝƸʣaCSF; in mM: 13.9 NaCl, 0.5 KCl, 0.13 MgSO4, 0.24 CaCl2, 0.124 KH2PO4, 1.5 NaHCO3, 1 glucoseʤ8ǀɄ%-¢DŽǟ7¯°ƽ8ɑı'K-Eʥ T^“T ”fV^eŽ”ZvŒ—’ʣAMI-6; WTb‡ʤO[TtZvŒ—’8ŪÃ%ʥ 5 µL 9Ƹɼ57KH8ɕŵ%- 1 nmol 9 CHA Ošœ%-šœ9ʉ:y‡ gl—O 228¸ƽ%-€’—s™8-°ƽ 32¦ɤB4¯š%3 I:ÊȱŁ8ǯÞ#)-y‡gl—9°ƽ¯š%ʥŭɅ8į'KœɳêŔ ƷĜ%3I:ʥŧȌǟ8°ƽOƾĦ'K-E8°ƽɇƾǒ€“—ʣML312; AD InstrumentsʤOȜɿI 2 cm 8ŪÃ%-€“—4ɇƾ#LK°ƽ 10 8ɮ%-Ɓǃ4y‡gl—OĤƘƥ%ʥȈȓOŬƍ%-Ĩʛ:ʥ€’—s ƽĿʥÊȱŁÆƽĿʥs”g„”m—ƽĿé=°ƽ€“—ƽĿO Power Lab ʣAD Instrumentsʥetv—, Y—gsRʤO¤%3b”}Œ—l—?ȉƁ ǟ8Ɋɽ%-  B-ʥǷ 2 ǵ4:ʥ¯°ƽ9ňʑOʆ- CHA šœȬ°8HK CIRP 9ɱŤ ǟg€Te”_?9ňʑOɕ@-+9-EʥCHA OšœŌʥ°ƽO 378 ȍŧ%-ȖO²Í%-ƌĨʛ4: CHA Ošœ'Kʉʥy‡gl—O 35-38 8¸ƽ%-€’—s9™8-s”g„”m—8HJ°ƽOƾĦ%7 Iʥ€’—s9ƽĿOɰħɕǻ%-CHA Ošœ%3I 4 ƁʁŌ8y‡gl —OĤƘƥ%ʥȈȓOŬƍ%-. . .

(15) T k~‘”õÃʟɷȲ8HK¢DŽǟ¯°ƽ9²Í  Ƿ 2 ǵ4:eR”y‡gl—ʥǷ 3 ǵ4: ddY Ȁ Ugé= SD Ȁos O³ǒ%ʥRrxe”Ȁ9ɚƴàO¤#(ʥ¯ƽ+9F9 CIRP ǝǍ8éA' ňʑOƖɈ%-+9-EʥÞLj8ʥʟɷȲO²ǒ#)ʥ°ƽɕǻOȶN7 NJŜ4ęɴIÊå%-Ĩʛ8:ʥ229ǏĕƽĿ83ʗȟ%3JĭÊʚ à%37ÞLjOǒ-"9ŹƮ8HJʥÞLj:ÉǥƁ8ńɝ"#LK Rrxe”Ȁ9ɚƴàO¤#(ʥŅÖǟ8¯°ƽ?ıÃ#LKʟɷȲ8:° ÆI9ūÍ9ɨõÃʟɷȲ9Tk~‘”ʣ~X—’”®õÃʟɷƸ; Abbot japanʥƐ¡ʥżƌʤOǒ-°ƽ€“—OȜɿI 2 cm B4ŪÃ%ʥ ǢȩƽĿOƾĦ%-#I8osǒőʍąʍƗʣv—^ˆrSZ‘ʥƐ¡ʥ żƌʤOǒ3ʥĖĺĀʣDAM50 Differential Amplifier; World Precision Instrument, ~“m, RˆZʤO¤%3őʍąOƾĦ%-°ƽO¯š#)Kʉ8:ʥB (ʥĻŰƽOö'KÞLjOTk~‘”ʣǂĿ 2ʢʥƵɨ 2.0 L/minʤOƵà #)3Kƒo^g8ǯÞ#)ʥ›Þà%-+%3ɨG8°ƽ€“—5 őʍą€“—OȹǦ%ʥñĨʛ8HKƽĿƎª?ǯÞ#)-ǯÞ8­ʥ Tk~‘”9õÃ: g^9ȹǦ8HJȶ0-ʣƵɨ 1.5 L/minʤĨʛ:ʥ ÞLj9Ǣȩƽʥőʍąé=ÊȱŁÆƽĿO Power Lab O¤%3b”}Œ—l—? ȉƁǟ8Ɋɽ%-  ƌŹƮ4:ʥÞLj9°ƽ¯š8­őŦŴ¯š'K+%3ʥʟɷO Ȍ. -Ēò8:Ɔȇǟ8őŦÞ¼ƞ'K+9-EʥőŦÞOŨƛ8Ƥʈǟ. 8Tk~‘”9ǂĿOš!-Tk~‘”9ǂĿ:őŦʨ300 ă/min 9Ɓ 8Tk~‘”ǂĿ 2ʢʥőŦ 200-300 ă/min 9Ɓ8 1.5ʢʥőŦ 150-200 ă/min 9Ɓ8 1.0ʢʥőŦ 100-150 ă/min 9Ɓ8 0.5ʢʥ őŦʧ100 ă/min 570-Ɓ 0ʢ 5%-ʣą 1ʤTk~‘”ǂĿOš!-ŌʥőŦŴ™ž%-Ɓ8:ʥ˜Ŀ . .

(16) Tk~‘”ǂĿO™!ʥÇĿőŦ¯š%3ITk~‘”ǂĿOš!-  Ƿ 2 ǵé=Ƿ 3 ǵ4:ʥɡĿ7¯°ƽ8HK CIRP ɲ¬Ġ9ǝǍ?9ňʑOɕ @K-Eʥ°ƽO 284ȍŧ%-ȖO²ŝ%-Ĩʛ4:ʥB( 258ɋĦ% -€’—s9™4ÞLjOÊå%-ÞLj9°ƽ 288ɮ%-I€’—s9ƽ ĿO 289ɋĦ8ĘƄ%-°ƽOɂĮ%7Iʥ€’—s9ƽĿOɰħɕǻ %-y‡gl—Oǒ-Ĩʛ4: 2 Ɓʁʥ UgOǒ-Ĩʛ4: 1 Ɓʁʥ °ƽO 28

(17) 183ȍŧ%-2883ȍŧŌɨG8ĤƘƥ%ȈȓOŬƍ%-  Ƿ 2 ǵ4:ʥy‡gl—83ʥɡĿ7¯°ƽ8HK CIRP ɲ¬ĠǝǍ9Ę àʥÉǥƚ9ƗĿ9¯°ƽ83Fȍŧ#LK96OƖɈ%-+ 9-Eʥ°ƽO 284ȍŧ%-Ōʥ10B4ŕÊ%-ȖO²ŝ%-Ĩʛ4:ʥ °ƽO 2883 2 Ɓʁȍŧ%-ŌʥɨG8 4-59ÊȱŁÆ?ǯÞ%ʥ°ƽ 1087KB4ÊåOȌ. -y‡gl—9°ƽ 108ɮ%-Ɓǃ4ĤƘƥ. %ʥȈȓOŬƍ%-  B-ʥǷ 2 ǵé=Ƿ 3 ǵ4:ʥɡĿ7ƽĿčOȃŽɧJɭKH8¯° ƽ?ɑı'K"58HK CIRP ɲ¬ĠǝǍ?9ňʑOɄƑ%-+9-Eʥ°ƽ O 10B-: 15B4ŕÊ%-ȖO²ŝ%-Ĩʛ4:ʥÞLj:ʟɷOõÃ# )›Þà%-ŌʥɨG8 4-59ÊȱŁÆ?ǯÞ%ʥ°ƽ 10B-: 15 87KB4ÊåOȌ. -Ƿ 2 ǵ83ʥy‡gl—:°ƽ 108ɮ%-. Ɓǃ4ĤƘƥ%ʥȈȓOŬƍ%-Ƿ 3 ǵ83 Ug:ʥ°ƽ 158ɮ %-Ɓǃ4ĤƘƥ%-  #I8ʥǷ 2 ǵ4:ʥɡĿ7¯°ƽOȉʛ%7Ēò9ʥƗĿ7¯°ƽ8H K CIRP ɲ¬ĠǝǍ?9ňʑOƖɈ%-+9-Eʥy‡gl—Oǒ3ʥ°ƽ O 10B4ŕÊŌ 6 Ɓʁȍŧ%-ȖO²ŝ%-Ĩʛ4:ʥy‡gl—:ʟɷ OõÃ#)›Þà%-ŌʥɨG8 4-59ÊȱŁÆ?ǯÞ%ʥ°ƽO 10B4 Êå%-ŌʥÊȱŁƽĿO 8±48ɋĦ%ʥ¯°ƽNJŜOȍŧ%-6 ƁʁŌ8 . .

(18) ĤƘƥ%ʥȈȓOŬƍ%-  Ƿ 3 ǵ4:ʥ Ug83ʥ¯°ƽ9ňʑOʆ-ʟɷȬ°8HK CIRP 9 ɱŤǟg€Te”_?9ňʑOɕ@-+9-EʥTk~‘”OõÃŌʥ °ƽO 378ȍŧ%-ȖO²Í%-ƌĨʛ4:ʟɷOõÃ#)›Þà%-Ōʥ ɨG8 UgO 35-388¸ƽ%-€’—s9™8-°ƽOƾĦ%7 Iʥ€’—s9ƽĿOɰħɕǻ%-Tk~‘”OõÃ%3I 2 ƁʁŌ8 UgOĤƘƥ%ʥȈȓOŬƍ%- . eR”y‡gl—9ĻŰƽƁ8. KʇāÆƽĿ9ƾĦ.  DžÌȔ9eR”y‡gl—8Tk~‘”OõÃ#)ʟɷ%-9/ʥƽĿ ɇƾǒ9s”g„”m—OʇāÆ8ŪÃ%-ʇāÆ4ɇƾ#L-ƽĿ:Ǿ Ķ9ƽĿOêƀ%3K5ș-B-ʥóƁ8ȪȨÆ8Ô9s”g„”m —OŪÃ%ʥȪė8ȒòǿOǒ3ĆĦ%-ȷŌ 1 ɫʁ:ăŏƉʁ5%- ĩƽ 22°CʥſƂøƉ 12 Ɓʁʦ12 ƁʁʣſƉ 7 : 00-19 : 00ʤ8ɋĦ#L-Ǐĕ 4ʗȟ%ʥʙ5ƫ:ȬǓ8Űë4KH8%-ʇāÆé=ȪȨÆ9ƽĿO 1 ż 1 ăʥſƉ83òɇ 7 żʁɇƾ%-. ĭÊǏĕ8ɉà%- Ug9²Í Ƿ 3 ǵ4:ʥ Ug8. KĭÊ×ǁ8HK CIRP ǝǍ?9ňʑOſI8'. K-E UgO 4-59ÊȱŁÆ4 1 ɫʁʗȟ%ʥĭÊǏĕʗȟȖO²ŝ%- ¯ƽǏĕ4 Ug: 1 á(1¹Ôʗȟ5%ʥʙ5ƫ:ȬǓ8Űë#)-. . .

(19) Ȉ ȓ9Ŭƍ  ĻŰƽƁ9eR”y‡gl—ʥ Ugé=osʥÉǥƁ9eR”y ‡gl—9ȦʥőȫʥȠʥȝȫʥȧȫé=ǾĶOŬƍ%-B-ʥ¢DŽǟ¯° ƽƁʥǨż–ĭÊƎª83ʗȟ%ʐÉǥƁ9ÞLj9Ȧé=őȫOŬƍ%-Ŭ ƍ9ʉ8:ʥÞLj8Tk~‘”ʟɷOź%ʥʕÞȤIŲȵƥ%-ȫĀ: Krebs’s Ƹʣin mM: 118.05 NaCl,4.69 KCl, 2.52 CaCl2, 1.16 MgSO4, 1.16 NaH2PO4, 25.0 NaHCO3, 1.11 Glucoseʤ4Ʋƶ%-ŌʥƸ°dzȃ4ŕǁ8ÊË%-CIRP ɲ¬Ġ9ɄƑʣŌɥʤOȶB4:ʥ-809ÊȱŁÆ4¸Ģ%-. RNA ťÍé= cDNA 9²ŝ  -8083¸Ģ%-ȦʥőȫʥȠʥȝȫʥȧȫé=ǾĶ9ȈȓLJȁ 50 mg Iʥ RNA ťÍ\osʣTRIZOL® Reagent; Thermo Fisher ScientificʥƐ¡ʥżƌB-: TRI Reagent; Molecular Research Center, Inc., YyTY, ǽćʤOǒ3ʥs—l ‘ RNA OťÍ%-˜ɴ9d”€‘813: TURBOTMDNase enzymes ʣThermo Fisher ScientificʤOǒ3ʥƺÃ%-ax‡ DNA 9ʆçOȶ0- ŎIL- RNA d”€‘9 260 nm 9Ưɾ8. KõÁĿOõÁĿɇ. ʣSpectrophotometer; HITACHIʥƐ¡ʥżƌʤ8HJƾĦ%-ƾĦ#L-õ ÁĿOF58 RNA 9ǂĿOǹÍ%-s—l‘ RNA 3 g IɦɠÈêŔO ȶʥcDNA Oòŝ%-ɦɠÈêŔ:ʥ”m‡€T —ʣThermo Fisher ScientificʤʥɦɠÈɸȃʣSuper Script® Reverse Transcriptase; Thermo Fisher ScientificʤOǒ3ʥd— ‘dT^—ʣGeneAmp® PCR System 2700; Thermo Fisher Scientificʤ83ȶ0-. . .

(20) € T —9²ŝ  Ż8đ÷9K|sʣNM_001280.2ʤʥ UgʣNM_007705.2ʤʥos ʣNM_031147ʤ9 CIRP mRNA 9ÅɧɶÑOèș8%3ʥCIRP ɲ¬Ġ9ɠÈLj 9ƖÍ9-E8ǒK€T —O²ŝ%-+9-E8ʥ ř#LKʀĞb t”OôD€T —O³ǒ%-Forward €T —O Cirp1ʣFʤʥreverse €T —O CirpʣRʤ5%3ȸ 1 8ǫ%-#I8ʥy‡gl—9 CIRP 9 open reading frame ʣORFʤÄɾOɄƑ'K-E8ʥ ř#LK ORF HJ™Ƶé=š Ƶ9ɶÑ8ǣȺǟ7€T —O²ŝ%-€T —: Ugé=os9 ɶÑOèș8²ŝ%-ORF Äɾ9e—^W”gɄƑ8³ǒ%- forward €T —O Cirp2ʣFʤʥreverse €T —O Cirp2ʣRʤ5%3ȸ 1 8ǫ%-. PCR Ʈ  Taq „ˆ—jʣPlatinum® Taq DNA Polymerase High FidelityʥT~q^ x“f—ʤOǒ3ʥcDNA êŔƸ 1 L I PCR Oȶ0-d— ‘dT^ —Oǒ3ʥÒƉĘŖ 94 2 Îʥ123 94 30 Ǯʥ55 30 Ǯʥ72 1 Î83ʥ28–35 dT^‘ʣŌɥʤʥ+9Ō 72 3 Î9ƽĿɋĦ83ǡǟ9ɶÑ Oŧ1 cDNA OĖĺ%-êŔƻ9Ƹ: 0.4 g/mL 9WnfU‡“ Ttʣu ZTqg^, ¡ɵ, żƌʤOôD 1.5%R[“—ga‘ʣuZTqg^ʤO ǒ3ʍƪƱÞOȶ0-ʍƪƱÞ%-a‘8:ʥȅęȐs”gT‘†w— l—ʣUVP Laboratory ProductsʥZ~X‘vRʥǽćB-: ChemiDoc™ XRS+ system; BIO RAD, Z~X‘vRʥǽćʤ8H03ȅęȐOdžİ%ʥêŔǐŝ LjOîɀà%-y‡gl—Oǒ-Ĩʛ8. K PCR êŔ9dT^‘Ŵ:ʥ. cDNA 9ǓƏ9ȫĀ8H03˜Ħ5%-"LI9dT^‘ŴOɋĦ'K-E8ʥ 19d”€‘4 25-40 dT^‘9 PCR OȶʥƖÍ#LKz”t9{l. . .

(21) —”8ɯ7"5OǪɐ%-+%3ʥPCR ǑLj9ĖĺƃȐ€s—8 ɮ#7dT^‘ŴOŬǒ%-y‡gl—83:ʥȦʦ28 dT^‘ʥő ȫʦ29 dT^‘ʥȠʦ28 dT^‘ʥȝȫʦ35 dT^‘ʥȧȫʦ29 dT^‘ʥ ǾĶʦ30 dT^‘8ɋĦ%- Ugé=osǓƏ9 cDNA 83:Ä3 9ȫĀ4 30 dT^‘8ɋĦ%-. Ĕ ďɶÑ9Ʉɔ  RT-PCR 8HJĖĺ%- CIRP cDNA 9ĔďɶÑOɄɔ%-y‡gl—9Ļ Ĺ°ƽƁé=ÉǥƁʥ Ug9ĻŰƽƁ9őȫ8ǓƏ'K RNA I²ŝ%cDNA ƸOʥCirp1ʣFʤé=ʣRʤOǒ3Ėĺ#)-ʍƪƱÞ%-ŌʥR[ “—ga‘™9ǡǟ9z”t9ɴÎ9 PCR ǑLjOÏJÍ%ʥmT’^se—^ W”gƮOȶ0-PCR ǑLj:eZˆ”’”ƮʣNucleoSpin® Gel and PCR Clean-upʥMACHEREY-NAGEL, rŒ—’”, tTpʤ83ǾȻ%-ǾȻLj9 ˜ɴOÇĿʍƪƱÞ%ʥǡǟ9z”t9CǾȻ#L3K"5OǪɐ%-Ōʥ \osʣABI Prism® Big Dye® Terminator v3.1 Cycle Sequencing KitsʥThermo Fisher ScientificʤOǒ3e—^W”gêŔOȶ0-d— ‘dT^—9ƽ ĿɋĦ:ʥ96 1 Îʥ123 96 10 Îʥ50 5 Îʥ60 4 ÎO 25 dT^ ‘83êŔOȶ0-+9Ō€T —Oʆç%ʥDNA ŷLJOǾȻ%-ǾȻ %-ŷLJ9Ʉɔ:ʥ ‘n\Š}—DNA e—`”d—ʣABI Prism 3100 Genetic Analyzer, Thermo Fisher ScientificʤO³ǒ%-  +9ŌʥORF ÄɾOɄƑ'K-E8ʥ ř#LK ORF HJ™Ƶé=šƵ9ɶ Ñ8ǣȺǟ7€T —Cirp2ʣFʤé= Cirp2ʣRʤ8H03Ėĺ%- PCR ǑLj 83e—^W”gɄƑOȶ0-Cirp1ʣFʤé=ʣRʤ8H03Ʉɔ%-ɶ ÑôBLK"5OǪɐ%ʥy‡gl—é= UgIƖÍ#L- cDNA ɶÑ. . .

(22) OƬĦ%-. Ǖ À9Ŵºàé=ȋɇÌǎ  ŎIL- PCR ǕÀ: Image J k~sUVRʣNational Institutes of Health, ˆ —”t, ǽćʤOǒʥȴÁŅĿOz”tƧ8Ŵºà%-˜19d”€‘ IŎIL-z”t9/ʥ800 bp ¦ɤ9z”tO long formʥB- 500 bp ¦ɤ9 z”tO short form 5%-Long form 9ŴºO short form 9Ŵº4Û0-F9 O long form/short form 5%3ǹÍ%ʥǝǍɼ9ƨOǫ%-  ŎIL-Ŵºr—l:Ä3ĻĊº ± ƛƿ»ĸʣStandard deviation; SDʤ4ȸɊ %-2 Ȗʁ9ƖĦ:ʥStudent’s t ƖĦB-: Welch’s t ƖĦOǒ-2 Ȗ¨ ™49ƖĦ:ʥDunnett’s ƖĦOǒ-P º < 0.05 9ĒòOȋɇģǟ8ƈŚ7 ĸK5%-ȋɇɄƑ8: Statcel4 k~sUVR ʣOMS, Đnj, żƌʤO ǒ-. . .

(23) ą 1ʦőŦŴ8HKTk~‘”ǂĿ9ɕǻ. Tk~‘”Oǒ3eR”y‡gl—O¢DŽǟ8¯°ƽ?ɑı'Kʉ8 :ʥőŦŴOŨƛ8%3ʥõÃ#)KTk~‘”9ǂĿOɕǻ%-őŦŴ ʨ300 ă/min 95:Tk~‘”ǂĿO 2%5%-óƚ8ʥőŦŴ 200-300 ă/min 95 1.5%ʥőŦŴ 150-200 ă/min 95 1.0%ʥőŦŴ 100-150 ă/min 95 1.0%ʥőŦŴʧ100 ă/min 958: 0%5%-. .

(24) ȸ 1ʦĨʛ8³ǒ%-€T —. €T —:ʥ|sʥ Ugé=os9 CIRP mRNA 9ÅɧɶÑI²ŝ%- Cirp1 9 forward é= reverse €T —:+L,LʀĞbt”é=ȇƞbt”O ôDCirp2 €T —: ř#LK ORF 9ę½9ɶÑ8Ȋò'KForward € T —OʣFʤʥreverse €T —OʣRʤ5ǫ'.  .

(25) Ƿ 1ǵ. eR”y‡gl—9ÉǥƁ8. K CIRP ɲ¬ĠǝǍ9ɄƑ.  ÉǥÞLj:ʥÉǥ8ƗĿ9¯°ƽ57K8FNI(ʥȮúǟ7ʊĪ: 7ƌĨʛ4³ǒ%-eR”y‡gl—83FʥÉǥ9¯°ƽƁ8 őȫ:ȿØƟ%ƳŋÞO¸03K"5ǧIL3Kʣ30ʤ"9"5 IʥeR”y‡gl—9Éǥ8:ʥ¯ƽ8HKʊĪOăɳ'K-E9Ɲƙ ĢĈ%3K5șILKƌǩDz4:ʥʊĪOăɳ'K-E9Ɲƙ9<5 15%3 CIRP ɲ¬Ġ8Ǧǡ%-Éǥ8 CIRP ƝȣOǝŮ'K5'L;ʥ eR”y‡gl—9Éǥ8:ĻĹƁ5ƨɢ%3 CIRP ɲ¬Ġ9ǝǍ™ž %3K5șILKB-ʥeR”y‡gl—9 CIRP ɲ¬Ġ9đ÷:Ɗ. 70--E CIRP ɲ¬Ġ9ǝǍK96OſI8'KŒȽ0 -+"4ʥƌǵ4:ʥeR”y‡gl—8. KĻĹƁ9 CIRP ɲ¬Ġ9ǝ. ǍNJŜOſI8'K"5ʥB-ʥÉǥƁ8 CIRP ɲ¬Ġ9ǝǍĘà'K9 6OſI8'K"5Oǡǟ5%- . .  .

(26) Ȋƒ. ĻŰƽȖ9eR”y‡gl—8. K CIRP mRNA ‚‰“_9Ǫɐ.  ĻŰƽȖ9eR”y‡gl—9ȦʥőȫʥȠʥȝȫé=ȧȫ83ʥ RT-PCR Ʈ8HJ CIRP ɲ¬ĠǝǍOƖȄ%-5"MʥÄ39ȫĀ83ʥ1 Ȉ9€T —Iȁ 500 bpʥ700 bpʥ800 bp 9 3 19dTh9 PCR ǑLjĖ ĺ#Lʣą 2ʤ ȁ 500 bp 9ǑLjO short formʥ ȁ 700 bp 9ǑLjO medium formʥ ȁ 800 bp 9ǑLjO long form 5%-ax‡ DNA 9ƺÃ9ƈDžOǪɐ'K-Eʥ ˜ɴ9d”€‘813: DNase ÌǎO%- RNA ƸI²ŝ%- cDNA 4 PCR êŔOȶ0-DNase ÌǎŌ9 RNA I²ŝ%- cDNA d”€‘83Fʥ óƚ8 3 DZʖ9 PCR ǑLjĖĺ#L-  őȫIĖĺ#L- PCR ǑLjOǒ3ʥe—^W”gɄƑOȶ0-5"Mʥ short form 9 ř#LK ORF ɶÑ:ʥ Ugʥosé=|s4đ÷#L3 K CIRP mRNA 9 ORF ɶÑ5ʞǣóŖOƈ%3-ǣóNj: Ug4 94ʢʥ os 93ʢʥ|s 89ʢ40-ʣą 3ʤB-ʥORF IR†xɹɶÑO ř %-y‡gl—5¥ÞLjDZ59R†xɹɶÑ9ǣóNj:ʥ Ug 99.4ʢʥ os 98.8ʢʥ|s 95.9ʢ40-ʣą 4ʤ%-03ʥshort form 5%3ƖÍ #L- mRNA :ʥCIRP mRNA 9‚‰“_4K5Ǫɐ#L-  B-ʥȁ 800 bp 9 PCR ǑLjʣlong formʤ9 ř#LK ORF :ʥshort form 5 ORF 9ʀĞǃI 502 bp :ĥÄ8˜Ȯ%3-˜Źʥ3’ ƋǶ½9ɶÑ8 212 bp 9ĔďɶÑŪÃ#L3-|s CIRP mRNA 9W\k”Oèș8ʥW\ k”ƙɩO ř%-5"Mʥlong form 9ɶÑ:ʥy‡gl—CIRP mRNA ‚‰“ _9Ƿ 6 W\k”5Ƿ 7 W\k”9ʁ8 CIRP mRNA 9T”s“”8-KɶÑ ŪÃ#L-Ň4K"5ſI570-ʣą 5ʤ"9Ƿ 6 W\k”5Ƿ 7 W\k”9ʁ8T”s“”8-KɶÑĢĈ'K5ƙɩ:ʥ Ugé= .  .

(27) |s83đ÷9KɠÈzR”sʣXM_006513165.1ʤé= ʣNM_001300829.2ʤ5˜Ȯ%3-y‡gl—83ʥŪÃ#L-ɶÑ8 : stop bt”ĢĈ%-%-03ʥlong form 9ɶÑI ř#LKR†x ɹ:ʥCIRP 5: C-ƋǶ9Ǚ7KF94KîȣŖǫþ#L-  ȁ 700 bp 9 PCR ǑLjʣmedium formʤ813:ʥmT’^se—^W”g ɄƑOɏC-ʥǑLj9ɼ short form G long form 5ƨɢ%3ijɼ4Jʥɶ ÑOƬĦ'K"5470-. É ǥȖ9eR”y‡gl—8. K CIRP mRNA ǝǍ.  ÉǥȖ9eR”y‡gl—9ȦʥőȫʥȠʥȝȫé=ȧȫ8. K CIRP. mRNA 9ǝǍO RT-PCR Ʈ8HJɄƑ%-Ä39ȫĀ83ʥÉǥȖ4: ä˜dTh9ȁ 500 bp 9 mRNA 8ǝǍʌȁ%ʥ+9¥9g€Te”_z R”s9ǝǍƼij'K"5ſI870-ʣą 6ʤĔďɶÑOɄƑ%-5 "Mʥȁ 500 bp 9ĖĺǑLj9ɶÑ:ʥĻŰƽȖ4ƖÍ#L- 3 DZʖ9ǑLj9 / short form 9F95ĥÄ8˜Ȯ%3-. ĻŰƽȖ5ÉǥȖ9eR”y‡gl—8. K CIRP mRNA ǝǍ9ƨ. ɢ  ĻŰƽȖé=ÉǥȖ9 CIRP mRNA ɄƑ83ƖÍ#L- short form é= long form 9z”t9ȴÁŅĿOŴºà%ʥshort form 5 long form 9ǝǍɼ9ƨ O long form/short form 5%3ǹÍ%-ĻŰƽȖé=ÉǥȖ9ŴºOƨɢ% -5"MʥȦʥőȫʥȠʥȝȫé=ȧȫ83ʥÉǥȖ4:ĻŰƽȖ8į %3ʥlong form/short form :ƈŚ8Ƽij%-ʣą 7ʤ1BJĻŰƽȖ4: short form 55F8 long form ǝǍ%3-ʥÉǥȖ4: short form 8ǝǍʌȁ %-"5ſI870-. . .

(28) ą 2ʦĻŰƽȖ9eR”y‡gl—8. K CIRP mRNA ɄƑ. ĻŰƽȖ9eR”y‡gl—9őȫʥȦʥȠʥȝȫé=ȧȫ83ǝǍ%3 - CIRP mRNA 9 RT-PCR ǑLj9ʍƪƱÞÀOǫ'Ȧʦ28 dT^‘ʥőȫʦ 29 dT^‘ʥȠʦ28 dT^‘ʥȝȫʦ35 dT^‘ʥȧȫʦ29 dT^‘83 RT-PCR Oȶ0-ÈǤƜ9Ŵº: —Z—9ĔďįʣbpʤOǫ'ȁ 500 bp 9ǑLjO short formʥȁ 800 bp 9ǑLjO long form 5%-§ȸ5%3 3 á9y‡gl— 8. KȊƒOǫ'. .

(29) ą 3ʦShort form b—t'K CIRP 9‚‰“_9 Y—€”—rS”_~’—‡ʣORFʤ8. KĔďɶÑ. eR”y‡gl—9őȫ83ǝǍ%3- mRNA 9/ʥshort form 9Y— €”—rS”_~’—‡ʣORFʤ9ɶÑOǫ'B-ʥŻ8đ÷9K U gʥosʥ|s9 CIRP 9 ORF ɶÑ5ƨɢ%-y‡gl—9ĔďɶÑ5 Ugʥosʥ|s9ĔďɶÑ59ǣóNj:+L,Lʥ94%ʥ93%ʥ89%40 -. .

(30) ハムスター MASDEGKLFVGGLSFDTNEQALEQVFSKYGQISEVVVVKDR マウス MASDEGKLFVGGLSFDTNEQALEQVFSKYGQISEVVVVKDR ラット MASDEGKLFVGGLSFDTNEQALEQVFSKYGQISEVVVVKDR ヒト  MASDEGKLFVGGLSFDTNEQSLEQVFSKYGQISEVVVVKDR. ETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQA ETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQA ETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQA ETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQA GKSSDNRARGYRGGSAGGRGFFRGGRSRGRGFSRGGGDRGY GKSSDNRSRGYRGGSAGGRGFFRGGRSRGRGFSRGGGDRGY GKSSDNRSRGYRGGSAGGRGFFRGGRSRGRGFSRGGGDWGY GKSSDNRSRGYRGGSAGGRGFFRGGRGRGRGFSRGGGDRGY GGGRFESRSGGYGGSRDYYASRSQGGSYGYRSSGGSYRDSY GGGRFESRSGGYGGSRDYYASRSQGGSYGYRSSGGSYRDSY GGGRFESRSGGYGGSRDYYASRSQGGSYGYRSSGGSYRDSY GGNRFESRSGGYGGSRDYYSSRSQSGGYSDRSSGGSYRDSY DSYATHNE DSYATHNE DSYATHNE DSYATHNE.  相同率  99.4 %  98.8 %  95.9 %. ą 4ʦShort form b—t'K CIRP ‚‰“_9R†xɹɶÑ. eR”y‡gl—9őȫ83ǝǍ%3- mRNA 9/ʥshort form 9 ORF ɶÑI ř#LKR†xɹɶÑOǫ'B-ʥŻ8đ÷9K Ugʥo sʥ|s9 CIRP 9R†xɹɶÑ5ƨɢ%-y‡gl—9R†xɹɶÑ5 U gʥosʥ|s9R†xɹɶÑ59ǣóNj:+L,Lʥ99.4%ʥ98.8%ʥ95.9% 40-. . .

(31) ą 5ʦeR”y‡gl—8. K short form é= long form 9W\k”ƙɩ. |s CIRP mRNA 9W\k”ƙɩOèș8ʥshort form 5 long form 9ƙɩO ř %ʥƨɢ%-:ʀĞbt”ʥ:ȇƞbt”Oǫ'Ŵġ:ʀĞbt” I9ĔďŴOǫ'_’—9ĂɃ4ǫ'ɴÎʥlong form 9/ short form 5 Ǚ7KɶÑ4K. .

(32) ą 6ʦÉǥȖ9eR”y‡gl—8. K CIRP mRNA ǝǍɄƑ. eR”y‡gl—9őȫʥȦʥȠʥȝȫé=ȧȫ83ʥÉǥ8ǝǍ%3 - CIRP mRNA 9 RT-PCR ǑLj9ʍƪƱÞÀOǫ'ą 2 5óƚ8Ȧʦ28 dT ^‘ʥőȫʦ29 dT^‘ʥȠʦ28 dT^‘ʥȝȫʦ35 dT^‘ʥȧȫʦ29 d T^‘83 RT-PCR Oȶ0-ÈǤƜ9Ŵº: —Z—9 bp Oǫ'§ȸ5% 3 3 á9y‡gl—8. KȊƒOǫ'. .

(33) ą 7ʦĻŰƽȖ5ÉǥȖ9eR”y‡gl—8. K. CIRP 9g€Te”_zR”sǝǍ9ƨɢ. ĻŰƽȖ5ÉǥȖ9eR”y‡gl—8. Kg€Te”_zR”s. 9ǝǍOĦɼǟ8Ɏ¶%-Short form 5 long form 9ǝǍɼ9ƨʣlong form/short formʤOĻĊº± SD 4ǫ'Ǟ9z—:ĻŰƽȖʣn=5ʤʥʠ9z—:Éǥ Ȗʣn=5ʤ9r—lOǫ'ȋɇ8: Student’s t ƖĦB-: Welch’s t ƖĦOǒ -**:ĻŰƽȖ5ƨɢ%3ʥÉǥȖȋɇǟ8ƈŚ40-"5Oȸ' ʣP < 0.01ʤ. .

(34) șĮ. ¯ƽeŽo^l”{^ɛ:ʥ¯ƽƁ8ǝǍ™ž'K"5ſI5703 KÎĠ4Kʣ6, 7, 35, 36ʤB-ʥĎʘȆȢOǒ-ǩDz8HJʥ¯ƽeŽ o^l”{^ɛ8:ȆȢO¸ɗ'K²ǒK"5ſI8#L3Kʣ5, 25, 39, 61, 64ʤ+"4ʥƌǩDz4: CIRP ɲ¬Ġ8Ǧǡ%ʥÉǥÞLj9°Æ8 3F CIRP 9ǝǍĖÜ%3K94:75 ř%-+"4ƌǵ4:ʥ ÉǥÞLj4KeR”y‡gl—9 CIRP ɲ¬Ġ9ǝǍOɄƑ%-ƌǵ9Ȋ ƒ8HJʥeR”y‡gl—83ʥĻŰƽƁ8 CIRP ‚‰“_Ob—t 'K mRNA ǝǍ%3K"5ʥB-ʥȼŴ9g€Te”_zR”sǝ Ǎ%3K"5ſI570-#I8ʥÉǥƁ8:Ɲȣǟ7 CIRP ‚‰“_ 8 mRNA 9ǝǍʌȁ'K"5ſI570-"9"5IʥÉǥÞLj9 ÉǥƁ8: CIRP ɲ¬Ġ9ǝǍ9äȂ7ĖÜ4:7ʥɱŤǟg€Te”_9 ɕǻO¤%-ʥǝǍˆZvh‡ĢĈ'K"5ǫþ#L-  ĻŰƽƁ9eR”y‡gl—I:ij75F 3 DZʖ9 PCR ǑLjĖĺ #L-+"4ʥPCR ǑLj9ɶÑOɄɔ%-Ȋƒʥshort form 5Ħȗ%-ȁ 500 bp 9ǑLj:ʥ|sʥ Ugé=os83Ż8đ÷9K CIRP mRNA 5ʞ ǣóŖOǫ%-%-03ʥshort form 5%3ƖÍ#L-ɶÑ: CIRP 9y‡ gl—‚‰“_4KîȣŖʞ|s CIRP 4:ʥRNA Ȋòɴ®4K ribonucleoproteinʣRNPʤ1 é= 2 9ɶÑGʥ+9ɶÑOôD RNA ȊòtˆT” ʣR†xɹ 1-84 ǘʤ9ɶÑƬĦ#L3Kʣ35ʤƌǩDz4ƬĦ%-eR ”y‡gl—9 CIRP ‚‰“_I ř#LKR†xɹɶÑ:ʥ|s CIRP 5ʥ RNP .. 47 RNA ȊòtˆT”Äč4ěɴΘȮ%-ʣą 8ʤ%-0. 3ʥy‡gl—CIRP Fʥ|s CIRP 5óƚ8ʥRNA Ȋòl”{^ɛ5%3Ɲȣ %3K5șILK .  .

(35)  ˜ȯǟ8ʥl”{^ɛ9ǝǍO™ž#)K-E8:ʥɲ¬Ġ9ɠÈO·ɬ' K"58HJ mRNA 9ɼOĖÜ#)ʥȘɌ#LKl”{^ɛ9ɼOĖÜ#)K ˆZvh‡șILK˜ŹʥÉǥƁ9eR”y‡gl—:ʥCIRP mRNA 9ǝǍOĖÜ#)K-E8Œ(%FɠÈɼOĖÜ#)KŒȽ:75șIL K7*7IʥɱŤǟg€Te”_OĘà#)K"59C4 CIRP l”{^ɛ 9ǝǍ9ÝNjOĖÜ#)ILKîȣŖKI4KÉǥƁ9H8ƽĿ 9¯š'KNJƭ83:ʥƚ7ɸȃƴŖ¯š'K-EʥɠȒ‘9Ę à:l”{^ɛ9ĖÜ8BJĬœ47îȣŖK+9-EʥɱŤǟ g€Te”_9ÏJƅ9C4ŒȽ7l”{^ɛ9ǝǍÝNjO™ž#)KƝ ƙ4K5'L;ʥěĘƈǒ4K£ŌʥĨʉ8 CIRP ɲ¬Ġ9ɠÈé= CIRP mRNA 9ǝǍɼ813Ħɼ%ʥɱŤǟg€Te”_9Ęàg€Te” _zR”s9ǝǍɼ869H7ňʑOéA'9ſI8'KŒȽK   ƌǵ4ſI8%- long form 9ɶÑ:ʥshort form 9Ƿ 6 W\k”5Ƿ 7 W \k”9ʁ8ʥCIRP mRNA 9T”s“”8ǣņ'KɶÑŪÃ#LKŇ40 -"9"5IʥCIRP ɲ¬Ġ9ǝǍ8:ɱŤǟg€Te”_ȶNL3 Jʥlong form : CIRP ɲ¬Ġ8ǓƏ'Kg€Te”_zR”s4K5ș ILK+"4ʥlong form l”{^ɛ?ȘɌ#L-Ēò9ƝȣO ƾ%- Long form b—t%3K5 ř#LKR†xɹɶÑ:ʥRNA ȊòtˆT” 4 CIRP 5ĥÄ8˜Ȯ%-+9-Eʥlong form IȘɌ#LKl”{^ɛF CIRP l”{^ɛ5óƚ8 RNA Ȋòl”{^ɛ4KîȣŖK%%ʥ long form b—t'KR†xɹɶÑ9 C ƋǶ: CIRP ‚‰“_9+L5:Ǚ7 K"5 ř#L-%-03ʥlong form IȘɌ#LKl”{^ɛ:ʥCIRP 5: C ƋǶ½9Ǵ°ƙɩǙ7KîȣŖKCIRP ƴŖà'K-E8:ˆ n‘àK:”ɹà#LK"5ŒȽ57Kʥ+L: CIRP 9 C ƋǶ4ɝ "K5đ÷Kʣ8ʤ+9-Eʥlong form IȘɌ#LKl”{^ɛ:ʥRNA .  .

(36) 8:Ȋò'KF99ʥˆn‘àK:”ɹà#L(8 CIRP 5%39ƴŖO F-7"5Oǫþ%3K%-03ʥĻĹƁ8: long form IȘɌ#L Kl”{^ɛ:Ɲȣǟ7 CIRP l”{^ɛ9t†u”sw[qS5%39ʼn ÛOƒ-%3K5șĮ%-1BJʥĻĹƁ8: CIRP 9ǝǍ:ɐEILKF 99ʥ+9Ɲȣ:ŠÖ#L3KîȣŖK˜ŹʥÉǥƁ9eR”y‡ gl—83ʥĻŰƽƁ8ĢĈ%3-ȼŴ9g€Te”_zR”s :ɂĮ#L(ʥCIRP 9‚‰“_9CǝǍ%3-"9"5IʥÉǥƁ8 :t†u”sw[qS5%3¿RTk~X—‡9ǝǍƼij'K-EʥƝ ȣǟ7 CIRP 9ƴŖà:ŠÖɄʆ#LK1BJʥĻĹƁ8: CIRP l”{^ ɛ:ĢĈ'KF99Ɲȣ:ŠÖ#L3JʥÉǥƁ8: CIRP 9Ɲȣ9ŠÖO Ʉʆ'K5NJŜřĦ4Kʣą 9ʤ%-03ʥɱŤǟg€Te”_ Oǒ- CIRP ǝǍƝľ:ʥÉǥƁ8 CIRP l”{^ɛ9ǝǍÝNjO™ž#)K .. 47ʥȃŽƝȣOǝŮ#)K-E8ƈǒ7ˆZvh‡4K5K. ʣą 9ʤ  ƌǵ9ɺȽ7Ȋƒ5%3ʥĻŰƽƁ5ÉǥƁ8. Kg€Te”_{l. —”9ĘàʥőȫʥȦʥȠʥȝȫé=ȧȫ5ƚ7ȫĀIÅɧ8ĢĈ 'K"5ſI570-"9ȊƒIʥɱŤǟg€Te”_9ˆZvh ‡:Ě9ȫĀ8Åɧ9F94K5șILKɱŤǟg€Te”_9ɕ ǻ8H03 CIRP l”{^ɛƚ7ȫĀ83¸ɗ݃OǝŮ%3K5 ƉŊ4K  ƌǵ4:ʥÉǥÞLj4KeR”y‡gl—83ʥÉǥƁ8 CIRP ɲ¬ Ġ9ǝǍ9Ęàɝ"K"5O ř%ʥCIRP ɲ¬Ġ9ǝǍɄƑOȶ0-Ȋƒ 5%3ʥeR”y‡gl—4:ĻĹƁé=ÉǥƁ96/I403F CIRP mRNA ǝǍ%3K"5ſI570-B-ʥĻĹƁ8:ȼŴ9g€ Te”_zR”sǝǍ%3JʥÉǥƁ8: CIRP mRNA ¨ę9g€Te .  .

(37) ”_zR”s9ǝǍƼij%3-1BJʥĻĹƁIÉǥƁ8:ɱŤǟ g€Te”_9ɕǻɝ"JʥÉǥƁ8: CIRP mRNA 9ǝǍ8ʌȁ%3- #I8ʥg€Te”_zR”s9ɶÑ:ʥCIRP 9t†u”sw[qS4 K"5Oǫþ%3K"5IFʥÉǥƁ9ǝǍƚŃ: CIRP l”{^ɛ9ǝ ǍÝNjé=ƝȣOʞEKîȣŖK. . .

(38) ą 8ʦShort form b—t'Ky‡gl—CIRP ‚‰“_5 |s CIRP l”{^ɛ9ƝȣǟtˆT”9ƨɢ. Short form IóĦ#L-eR”y‡gl—9 CIRP ‚‰“_9ƙɩOʥ|s CIRP 4Ż8đ÷9KƝȣǟtˆT”5ƨɢ%-|s CIRP 9ʀĞbt” I 1-84 ǘǡ9R†xɹ: RNA ȊòtˆT”4Klj8 RNPʣribonucleoproteinʤ 1 é= RNP2 : RNA Ȋò‰n—~5%3ǐLjDZʁ4Ľ¸Ģ#L3KɶÑ4 K84 ǘǡ¨ʄ9R†xɹʓč:ʥ_e”onʓč4KǞ9z—: R†xɹɶÑOǫ'|s CIRP 5y‡gl—CIRP 83ʥǙ7KR†xɹ 9COȸɊ%ʥǞţ9ɴÎ:ǣó4K"5Oǫ'. .

(39) ą 9 : eR”y‡gl—8. KɱŤǟg€Te”_9ɕǻOǒ-. CIRP l”{^ɛǝǍˆZvh‡. ƌǩDzIșĮ4K CIRP l”{^ɛǝǍƝƙ9©ɓOą4ǫ'ĻŰƽƁ 8:KǰĿ9ɠÈOȶʥȼŴ9g€Te”_zR”s8Îų'K" 58HJ CIRP l”{^9òŝɼGƝȣ¯ɕŵ#L3KÉǥƁ8: CIRP l”{^ɛOb—t'K mRNA ?g€Te”_Oʌȁ#)K"58H03Ș ɌGƝȣOʞE3KîȣŖǫþ#LK"9Ɲƙ8H03ʥÉǥƁ9H 7ŕǁ7ƽĿĘà8ɨG8įŔ4K5ș-. .

(40) Œ 2ő. LiAlZdNPm)< CIRP *ƙúŀN_hBLlH*Æ¥Ž»*ēƃ.  Œ 1 ő%+ƷŴľŀ(˜ńć) CIRP ƚ~ÌN_hBLlHƈŖ@­< &Ć:&(!˜ńć)çƍ=< CIRP *ƙúŀN_hBLl HƈŖ*Ž»@Ć:)Ʒ–IJ<&+Ʒxì*ĵijęŬ@ēƃƷƨ ˜ń¢į.ðķ$ 5)ƝŽ% <%Čő%+Ʒ˜ńć*ƙúŀ N_hBLlH*Æ¥@çƍŽ»@Ć:)<&@Łŀ&  LiAlZdNPm@˜ń<5)+ňăĎ}«-ؙĴÄ)$ƯŪ <&ŽĜ=<Ƶ4, 19ƶ:)Ʒ*9

(41) (ĴÄ%ƯŪ<&)9; ˜ń *ĩ&$`jel›ğ7ƚ~ÌľIJ)Ė (Æ¥çƍ=< &Á¶=$ <Ƶ2, 14, 41, 54ƶ 2Ʒ˜ńć)‚ƒĦ.ے<ƣ) +ٕ%ASYLl A1 ­Õƒ@yLHVjïƬ% <Ƶ23, 50ƶ * 9

(42) (˜ń *Æ¥«-˜ńےć*LHVj)ŅŁƷCIRP *ƙúŀN_ hBLlH)êƪ<*'

(43) @Ć:)<&&*5)ƷØ ™ĴÄ%ƯŪZdNPm2+ASYLl A1 ­ÕƒAJWNT@Ůӕ øq‚ƒĦ.ƇÛZdNPm* CIRP *N_hBLlHľIJ@Ɓď :)ƷƳƜŹ@ķ $‚ƒĦ.ƇÛ<&)9;Ʒ˜ń)ơq<A SYLlŘ*LHVj@yƷ‚Ħ*6*)9!$˜ńĖ* CIRP *ƙú ŀN_hBLlH@–IJ%<'

(44) ēƃ.  . .

(45) ŝĐ. ؙ«-ňăĎ}ƯŪ)9< CIRP mRNA *N_hBLlHľIJ.*êƪ  ؙĴÄ«-ňăĎ}ƯŪ CIRP *ƙúŀN_hBLlHƈŖ)êƪ@q <*'

(46) @Ɓď<5Ʒ˜ń$ ( 6**ؙ«-ňăĴÄ%Ɵ ĉƠƯŪƷؙ«-ňăĴÄƯŪť*ZdNPm@ķ $ CIRP mRNA * Ɓď@Ż!=:*ZdNPm&²PBclH%ؙ«-ňăĎ}%Ư ŪZdNPm* 50%|o+˜ń@ŜƱ˜ńZdNPm+Œ 1 ő ) $Ʒ˜ń‰ƒ&$Ɓď) ķRT-PCR *ŝĐƷؙ«-ňăĴ Ä%ƯŪť*ZdNPm*Ů«-îų: 3 Č*[lUēš=Ƶ¼ 10ƶ2Ʒshort form & long form *ľIJƞ*Ě@ŕšƷ¼ 11 )2&5 ؙ«-ňăĴÄƯŪť&áàƒĦť*ZdNPm) $ƷĈõ(ß+Ɔ5 :=(!. ASYLl A1 ­ÕƒAJWNTƵCHAƶ@ķ wĬŀ(‚ƒĦ)9< CIRP mRNA *N_hBLlHľIJ.*êƪ  7 ¦*ZdNPm) $ƷCHA *Ůӕøq&ƒżƩ:*™©@ś3± ?<&)9!$‚ƒĦ.ƇÛ/$*ZdNPm*ƒĦ*ŜćŀÆ ¥@¼ 12 )ŋ4 ¦*ZdNPm%+ƷCHA øq:ƷƒĦ 10) Ɨ<2%) 350-465 ›Ƶ400±42 › n=4ƶ@ŽÒƣ*Ŵľŀ(˜ń) $*‚ƒĦƗćƠ6ř 500 ›Ŏã% <Ƶ19, 50ƶ5Ʒ=:*ZdN Pm*ƒĦ‚pƔã+Ŵľŀ(˜ń&Ʈ$ :)Ʒ=:*ZdN Pm+Ŵľŀ(˜ń&²Ė)Ʒƒ@2<5Ë£@µ$ nĂƷ3 ¦* ZdNPm+ƷĚƐŀŚĄ ƒĦ‚p@ŋ$;Ʒ10)Ɨ<2%) !ćƠ+ 165-215 ›Ƶ193±20 › n=3ƶ% !ƒĦ‚p)ćƠ@Ž  . .

(47) 4 ¦@ Slow ťƷŚĄƒĦ‚p 3 ¦@ Fast ť&Slow ť& Fast ť * 10ƗćƠ)+ŞƂŀ)Ĉõ(ßƆ5:=RT-PCR )9;ƷƒĦ‚ pƔã)?:/$*ZdNPm) $Ʒîų%+ short form *3 ēš=Ƶ¼ 13ƶŮ) $+ƷÒƱ)‡ 7 ¦*ZdNPm*

(48). Ʒ. 10Ɨ2%*ćƠĈõ)Ɵ! Slow ť%+Ʒshort form .*ƥřƆ5 :=Ƶ¼ 13 slow ťƶnĂƷ Fast ť*ZdNPm%+ƷŮ) $+ short form &&6) long form *ľIJƆ5:=Ƶ¼ 13 fast ťƶnĂƷCHA @ø qìƷƒĦ@ 37)şûť%+Ʒîų«-Ů) $ƷƙúŀN_hBL lH*\Pml*Æ¥+çƍ=(!Ƶ¼ 14, 15ƶ  2Ʒshort form & long form *ľIJƞ*Ě@ŕšƷáàƒĦť*ZdNP m&ĚƐîų) $+ƷCHA @øq‚ƒĦ&(! Slow ť«Fast ť%ƷĈõ(ßƆ5:=Ƶ¼ 15ƶŮ) $ƷSlow ť%+îų&² Ė)ƷáàƒĦť&ĚƐŞƂÎŀ)Ĉõ)Æ¥$ Ƶ¼ 15 slow ťƶ n ĂƷCHA @øqĚƐŀŚĄƒĦ‚p Fast ť%+ƷŮ)< long form/ short form Ě+áàƒĦť&ĚƐ$Ĉõ(ß+Ɔ5:=(!Ƶ¼ 15 fast ťƶ2ƷCHA @øqìƷƒĦ@ 37)şûť) $+îųƷŮ &6)ƷáàƒĦť& long form/ short form Ě)ß+(!. Ə ã(‚ƒĦ)9< CIRP mRNA *N_hBLlHľIJ.*êƪ  CHA @ķ wĬŀ‚ƒĦ%+ƷŮ) $N_hBLlH*ľIJ) 2 "* \PmlƆ5:=$Ʒĺ(<ľIJ\Pml@ŋZdNPm+Ʒƒ Ħ*‚pƔã)6Ƙ  ;Ʒ8!;&ƒĦ‚p@ŋ±)*3˜ ńĖ* CIRP *ƙúŀN_hBLlHƆ5:=8!;&ƒĦ‚p @±)+ƷŚĄ‚p±9;Ɵ ćƠƷƏã(ĦãÀ@ŜƱ <!$ƷƏã(‚Ħ*6* CIRP ƚ~Ì@ƈŖ<®Ŭò@ēƃ  . .

(49) %ƷASYLl@y( ĂĠ% ;Ʒ"ƷÇƛ*Ħã@ƈŖ <&)9;Ʒ¢į*ƒĦ@þ„<&%<ƳƜŹ@ķ ‚ƒĦƇÛ Ġ@ķ 28zƒ2%*‚ƒĦ@Əã*‚ƒĦƷ10*ƒĦ@Ĕã*‚ƒ Ħ&ÑŦƷƏã(‚ƒĦ)9!$ CIRP Æ¥<*'

(50) ƈ/22 *ÓĦ%ƯŪ$ ZdNPm@ƷƳƜ&™©*ś3±?%wĬŀ)‚ƒ Ħ.ƇÛČĂĠ)9<łűĦ*Æ¥@¼ 16 )ŋwĬŀ)ƒĦ@ 28%şûť%+Ʒîų«-Ů) $ƷN_hBLlH[iAlT*

(51) short form £&(<˜ńć*\Pmlēš=Ƶ¼ 17 Kl_j 3ƶ2Ʒshort form & long form *ľIJƞ*Ě@ŕš&>ƷáàƒĦť *ZdNPm&ĚƐƷĈõ(ßƆ5:=Ƶ¼ 18 Kl_j 3ƶƒĦ@ 28%şûìƷ102%ñ™ť) $6²Ė)Ʒ˜ńť&Ʈ ƷN_hBLlH short form .ƥř\Pmlēš=Ƶ¼ 17 Kl _j 4ƶ2ƷáàƒĦť&ĚƐƷlong form/ short form Ě)" $6ŞƂÎ ŀ)Ĉõ)Æ¥$ <&Ɔ5:=Ƶ¼ 18 Kl_j 4ƶ=:*Zd NPm*łűĦ%ħуĦ 28.Ɨ<2%*ćƠ+ƷBO^jhl @´’Ê5$: 40-70 ›Ƶ51±8 ›, n=10ƶì% !  2ƷZdNPm*ƒĦ@ 28%şûƷñƔ) 102%‚p ±) CIRP *N_hBLlHÆ¥<'

(52) @ēƃZdNPm) BO^jhl@´’ìƔ7) 4)şû™Ÿä)ō¢& >Ʒ80-185 ›Ƶ111±31 ›, n=9ƶì)ƒĦ 10)Ɨ*ĂĠ%+ 3228*ĦãÀ)ν<ćƠ+ 8-10 ›Ƶ8.9 ± 1.0 ›, n=9ƶ!Ƶ¼ 16ƶƒ Ħ@ 102%ñ™ť%+Ʒîų«-Ů) $Ʒshort form .*ƥř+Ć :)Ɔ5:=(!Ƶ¼ 17 Kl_j 1ƶ Long form/ short form Ě)" $ 6ƷáàƒĦť&ĚƐƷŞƂÎŀ)Ĉõ)Æ¥$ <&+Ɔ5:=( !Ƶ¼ 18 Kl_j 1ƶ2ƷƟćƠ*Ĕã*‚ƒĦ@ŜƱ<&)9;  . .

(53) CIRP *ƙúŀN_hBLlHÆ¥<*'

(54) @ƈ/ƒĦ@ 102 %ñ™ì 6 ćƠşûť%+Ʒîų«-Ů) $ƷN_hBLlH[iA lT* short form .*ƥř+Ć:)Ɔ5:=(!Ƶ¼ 17 Kl_j 2ƶ Long form/ short form Ě)" $6ƷáàƒĦć&ĚƐƷĈõ)Æ¥$ < &+Ɔ5:=(!Ƶ¼ 18 Kl_j 2ƶ  ŗ Ý*Ħã«- CIRP mRNA *N_hBLlHľIJ  ÓĦ 22%ƯŪLiAlZdNPm*ŗÝ*Ħã@ƷƢº•)ƄŤ ƒĦƂħķThlNalQm)9;Ƃħ&>Ʒ32.2±3.0% !Ų Ű•)ƄŤThlNalQm)9;ƂħŲەĦã+Ʒ36.6±0.8%. !2ƷRT-PCR )9; CIRP mRNA *N_hBLlHľIJ@Ɓď &>ƷîųƷŮƷūƷũų«-ůų*ľIJ&+ĺ(;ƷŗÝ%+áàƒĦť ) $6 long form *ľIJ+Ü(Ʒ short form ÈľIJ$ Ƶ¼ 19ƶ 2ƷáàƒĦť&˜ńť)N_hBLlH\Pml*Ƙ +Ɔ5:= (!Ƶ¼ 19ƶLong form/ short form Ě)" $6ƷáàƒĦť&ĚƐƷ ŞƂÎŀ)Ĉõ)Æ¥$ <&+Ɔ5:=(!Ƶ¼ 20ƶ.  . .

(55) ¼ 10Ƹؙ«-ňăĴÄƯŪť*ZdNPm)< CIRP mRNA ľIJƁď. ؙ«-ňăĴÄƯŪť*LiAlZdNPm)<îų«-Ů)ľIJ$  CIRP mRNA * RT-PCR Ķį*Ʀěġ¢Ž@ŋŮƸ28 KBGjƷî ųƸ29 KBGj)$ RT-PCR @Ż!—ŃĘ*ÿŠ+bmDm* bp @ŋ {ż&$ 3 ¦*ZdNPm)<ŝĐ@ŋĚƐÚƊ&$áàƒ Ħť«-˜ńťƷ== 1 ¦*ŝĐ@Þ)ŋ. .

(56) ¼ 11Ƹؙ«-ňăĴÄƯŪť)<N_hBLlH[iAlTľIJ*Æ¥. °‰ƒ*îų«-Ů)< short form & long form *ľIJƞ*ĚƵlong form/short formƶ@ŕšáàƒĦťƵn=5ƶƷ˜ńťƵn=5ƶ«-ؙ«ňăĴÄƯŪťƵn=7ƶ @ᾊ± SD %ŋÈƝĚƐ)+ Dunnett’s Ġ@ķ ƷáàƒĦť&==*ť*Š@ĚƐ**+áàƒĦť&ĚƐ$Ş Ƃŀ)Ĉõ% !&@żƵP < 0.01ƶ. .

(57) ¼ 12ƸCHA @ķ $‚ƒĦ.ƇÛLiAlZdNPm)< ƒĦ*ŜćŀÆ¥. LiAlZdNPm*Ůӕ)ASYLl A1 ­ÕƒAJWNTƵCHAƶ@ø q$:*ƒĦ*ŜćŀÆ¥@ŋƴÃ;*bmG%ŋťƵFast ťƶ &Ŀù*bmG%ŋťƵSlow ťƶ*ƒĦ 10)Ɨ<2%*ćƠ )+Ĉõ(ß !. .

(58) ¼ 13ƸCHA @ķ $‚ƒĦ.ƇÛLiAlZdNPm)< CIRP mRNA ľIJƁď. CHA @øq™©LiAlZdNPm)<îų«-Ů)ľIJ$  CIRP mRNA * RT-PCR Ķį*Ʀěġ¢Ž@ŋŮƸ28 KBGjƷîųƸ29 KBGj)$ RT-PCR @Ż!—ŃĘ*ÿŠ+bmDm* bp @ŋĚƐ &$áàƒĦć«-˜ńć* PCR Ž@ŋSlow ť+ 10Ɨ2%*ćƠ Ĉõ)Ɵ!ťƷ2ƷFast ť+ 10Ɨ2%*ćƠň!ť% < PCR Kl_jp*bmG+Ʒ¼ 12 ) $ƒĦ‚p@ŋ‰ƒ&²n%. <ĚƐÚƊ&$áàƒĦť* PCR Ž@ŋ. .

(59) ¼ 14ƸCHA øqìƒĦ@ 37)şûť)< CIRP mRNA ľIJƁď. CHA øqìƷƒĦ@ 37% 4 ćƠşûLiAlZdNPm)<Ʒî ų«-Ů)ľIJ$  CIRP mRNA * RT-PCR Ķį*Ʀěġ¢Ž@ŋ ŮƸ28 KBGjƷîųƸ29 KBGj)$ RT-PCR @Ż!—ŃĘ*ÿŠ+ bmDm* bp @ŋ{ż&$ 2 ¦*ZdNPm)<ŝĐ@ŋĚ ƐÚƊ&$áàƒĦť«-˜ńť*ŝĐ@Þ)ŋ. .

(60) ¼ 15ƸCHA øq)9< CIRP N_hBLlH[iAlTľIJ*Æ¥. °‰ƒ*îų«-Ů)< short form & long form *ľIJƞ*ĚƵlong form/short formƶ@ŕšáàƒĦťƵn=5ƶ&Ʒ˜ńťƵn=5ƶ ƷSlow ť Ƶn=4ƶ ƷFast ťƵn=3ƶ«- CHA @øqìƷƒĦ@ 37)şûťƵn=4ƶ @ᾊ SD %ŋÈƝĚƐ)+ Dunnett’s Ġ@ķ ƷáàƒĦť&= =*ť*Š@ĚƐ**+áàƒĦť&ĚƐ$ŞƂŀ)Ĉõ% !& @żƵP < 0.01ƶ. .

(61) ¼ 16ƸBO^jhl@ķ $‚ƒĦ.ƇÛLiAlZdNPm)< ƒĦ*ŜćŀÆ¥. LiAlZdNPm)BO^jhl@´’™©±*ƒĦ*ŜćŀÆ ¥ŋƒĦ@ 28%şûť* 1 †@ƷƒĦ@ 28%şûìƷ 102%ñ™ť* 1 †@%bmGHh^%ŋ2ƷƒĦ@ 10 2%ñ™ť* 1 †@ƷƒĦ@ 102%ñ™ì 6 ćƠşûť* 1 †@ %bmGHh^%ŋ¼r*ņ¨+ZdNPm*śţ@üčPB clH@ŋ$;ƷĹ¯+¼ 17 «- 18 *Kl_jĹ¯&nŵ$ <. .

(62) ¼ 17ƸBO^jhl@ķ $‚ƒĦ.ƇÛLiAlZdNPm)< CIRP mRNA ľIJƁď. BO^jhl@ķ $wĬŀ)‚ƒĦ&LiAlZdNPm)<Ʒî ų«-Ů)ľIJ$  CIRP mRNA * RT-PCR Ķį*Ʀěġ¢Ž@ŋ ŮƸ28 KBGjƷîųƸ29 KBGj)$ RT-PCR @Ż!—ŃĘ*ÿŠ+ bmDm* bp @ŋKl_j 1 +ƷƒĦ@ 102%ñ™ťƷKl_j 2 +ƒĦ@ 102%ñ™ì 6 ćƠşûťƷKl_j 3 +ƒĦ@ 28%şû ťƷKl_j 4 +ƒĦ@ 28%şûì 102%ñ™ť:Ʒ =={ż* 1 †@ŋĚƐÚƊ&$áàƒĦť«-˜ńť*ŝĐ@Þ) ŋ. .

(63) ¼ 18ƸBO^jhl@ķ $‚ƒĦ.ƇÛLiAlZdNPm)< CIRP N_hBLlH[iAlTľIJ*Æ¥. °‰ƒ*îų«-Ů)< short form & long form *ľIJƞ*ĚƵlong form/short formƶ@ŕšáàƒĦťƵn=4ƶ&Ʒ˜ńťƵn=4ƶ ƷƒĦ@ 102%ñ™ťƵKl_j 1Ƹn=4ƶƷƒĦ@ 102%ñ™ì 6 ćƠşû ťƵKl_j 2Ƹn=5ƶƷƒĦ@ 28%şûťƵKl_j 3Ƹn=5ƶ«-ƒ Ħ@ 28%şûì 102%ñ™ťƵKl_j 4Ƹn=5ƶ@ᾊ SD %ŋÈƝĚƐ)+ Dunnett’s Ġ@ķ ƷáàƒĦť&==*ť*Š@Ě Ɛ**+áàƒĦť&ĚƐ$ŞƂŀ)Ĉõ% !&@żƵP < 0.01ƶ. .

(64) ¼ 19ƸáàƒĦť«-˜ńť*LiAlZdNPm)< ŗÝ* CIRP mRNA ľIJƁď. áàƒĦť«-˜ńť*LiAlZdNPm) $ŗÝ)ľIJ$  CIRP mRNA * RT-PCR Ķį*Ʀěġ¢Ž@ŋRT-PCR + 30 KBGj)$ Ż!—ŃĘ*ÿŠ+bmDm* bp @ŋ{ż&$°ť 3 ¦*ZdN Pm)<ŝĐ@ŋ. .

(65) ¼ 20ƸáàƒĦť&˜ńť*LiAlZdNPm)<ŗÝ* CIRP *N_hBLlH[iAlTľIJ*ĚƐ. áàƒĦť&˜ńť*LiAlZdNPm) $ŗÝ)ľIJ$ < short form & long form *ľIJƞ*ĚƵlong form/short formƶ@ᾊ± SD %ŋ Ŀ*[m+áàƒĦťƵn=5ƶƷƴ*[m+˜ńťƵn=5ƶ*SmP@ŋŞ Ƃ)+ Student’s t ēÑ@ķ **+áàƒĦť&ĚƐ$Ʒ˜ńťŞƂŀ )Ĉõ% !&@żƵP < 0.01ƶĚƐ&$Ʒîų)<áàƒĦć «-˜ńć*SmP@ŋ. .

(66) ŧÙ.  Œ 2 ő%+ƷŴľŀ(˜ńć)Ɔ5:= CIRP ƚ~Ì*ƙúŀN_hBL lH*Æ¥@çƍŽ»@Ć:)<&@Łŀ&*5Ʒ˜ ń¢į% <LiAlZdNPm@ķ $Ė (Ď}p% CIRP *N_hBL lHľIJ*Ɓď@Ż!*ŝĐƷÒƣ)‚ƒĦ&(!$ ( ±)+Ʒ ؙĴÄ«-ňăĎ}.*ƫ¥7ASYLl A1 ­Õƒ@yLHVj)9 !$ƷƙúŀN_hBLlH*Æ¥+çƍ=( &Ć:&(! nĂƷƏã(‚ƒĦ@§›(ćƠşû<&%ƷCIRP *ƙúŀN_h BLlH*ľIJ\Pml˜ńĖ)Æ¥Ŵľŀ(˜ń) $ƷLiA lZdNPm+8!;&ƒĦ‚p@µ<5ƷƏã(‚ƒĦ@ÿćƠ ŜƱ$ <&ŧ :=<*&:ƷLiAlZdNPm*Ŵľŀ˜ń ) $6ƷƏã(‚ƒĦ)9;ƙúŀN_hBLlHÆ¥<®Ŭòŋ ¸=<  =2%Ʒ˜ńć*‚ƒĦ)Ú$Ũò@ıí<5)+Ʒ˜ń *ĉƠ )<Ė (ų¹%*ƚ~Ì*ľIJÆ¥«-=)

(67) ieSilHïŽ % <&ŧ :=$† ,Ʒ˜ń¢į%+ƒĦ‚p)Ő!$ƷîŔ* FfR_MflGLgl@éö<IXELl 43 *ľIJoąƷîŔ*¬ š)ơq<TkaWl C *ľIJ‚p$ <œ*Á¶ <Ƶ2, 14, 41ƶ !$Čʼnŏ) $6ƷCIRP *ƙúŀN_hBLlH*Æ¥Ʒ˜ń ć*ƒĦ‚p)Ő!$çƍ=$ <®Ŭò@ēƃƷŝĐ &$Ʒؙ«-ňăĎ}%*ƯŪ*3%ƷÒƣ)ƒĦ‚p*ƍ!$ ( ZdNPm) $+Ʒ˜ńć)ƀÙ=9

(68) ( short form * CIRP mRNA .*ƥř+ŊƆ=(!²ĉƠƷ²ĴÄ%ƯŪZdNPm˜ ń$ <&:Ʒ*ĴÄ:q :=<ĦãŸī«-‘Ÿī+Ʒ <Ŏ  . .

(69) ã“Ǝ*`jel7ŌŜŀ(Æ¥@6:$ <+% <*ÒƱ)‡ Ʒؙ«-ňăĎ}%ƯŪZdNPm˜ń *ĩ@Ðu$  '

(70) )" $+Ć:%+( 6**ƷÜ(&6ƛ›ŀ)+˜ń< 5*Æ¥@ŜƱ$ <&ŧ :=<2ƷCHA 2+BO^jhl@ķ wĬŀ(ĂĠ%ƒĦ@‚pÒƱ%+ƷؙĴÄ%ƯŪ$ ( Zd NPm@ķ 6**Ʒ‚Ħ*Ď})9!$+ CIRP *N_hBLlH+˜ń Ė)Æ¥$ <*&:6Ʒؙ«-ňăĎ}+ CIRP *N_hBL lH*Æ¥)ïƬ%+( &ŋ¸=  LiAlZdNPm*Ŵľŀ(˜ń)+Ʒ•Œſâpƛ*ASYLl A1 ­ ՃÅ¡<&ƝŽ% <&Á¶ <ƒĦ‚p*5)ƷASYL l A1 ­Õƒ+ Gi źĿ”ë¿­Õƒ&$Ʒſâpƛ) $ċĒ:*Ĝî òLHVj@÷žƷĮĶĵęĕ@÷ž<Ƶ50ƶ *Ňž+ƷŴľŀ(˜ ń@Ż!$ <ZdNPm7MiN*ٕ)ASYLl A1 ­Õƒ*AlPJ WNT@øq<&Ŵľŀ(ƒĦ‚prĀ<&

(71) ÒƱ)9!$ƅĆ= $ <Ƶ23, 50ƶ*9

(72) )ƷŴľŀ(˜ń*‚ƒĦ.*ے)+ASYLl Ř*ƋĢ¥ƝŽ% <&:ƷČʼnŏ) $6 CIRP *ƙúŀN_hB LlH*Æ¥)ASYLl)9<êƪ <&vô%Ʒ ASYLl A1 ­Õƒ@İĺŀ)Ģò¥<ķƞ* CHA @ŮӕøqƷLiAlZdN Pm@˜ńĖ*‚ƒĦ.ƇÛ<ĂĠƵ31ƶ@ķ $ CIRP ƚ~Ì*Ɓď@Ż !îų) $+ CHA @øq‚ƒĦ&(!±)+Ʒvô&nŵ $ CIRP *N_hBLlH+Æ¥%Ʒ6ASYLlŘ*ƋĢ¥ *6*êƪ<&=,Ʒ‚ƒĦ)ƇÛ<&(Ʒ37)şû± %6 CIRP *N_hBLlHÆ¥<&vôƷCHA @øqì) 37)şû±)+ CIRP *N_hBLlHÆ¥+Ɔ5:=(!" 2;Ʒ‚ƒĦ&(!±)+ CIRP *N_hBLlH+Æ¥<6**ƷA  . .

(73) SYLl A1 ­ÕƒLHVj*rđ%*Ģò¥*3%+ƙúŀN_hBLlH *Æ¥+çƍ=( &Ć:&(!  CHA @Ůӕ)øq‚ƒĦ&(!ZdNPm*Ů) $ƷƒĦ‚p Ĉõ)Ą ZdNPm%+ƷCIRP *N_hBLlH+˜ńĖ*Æ¥@ƍ (!Ŷ·Ĥ &)ƷbCN*ģưNhBNėČ@ķ ʼnŏ) $Ʒ33zƒ*‚Ħp%+ CIRP mRNA *ľIJÅ¡<nĂƷ17zƒ%+ ľIJÅ¡( &

(74) Á¶ <Ƶ52ƶ*&:ƷƒĦ‚pƔã*Ƙ ƙúŀN_hBLlH)êƪ@«1$ <®Ŭò <&ŧ ƒĦ ‚pĄ ±ƷƏã(‚ĦÀ%*νćƠ+ňƷƒĦ‚pƕ ±)+ Əã(‚ĦÀ%*νćƠ+Ɵ "2;ƷƏã(ĦãÀ%*νćƠ)9! $ƙúŀN_hBLlH*Æ¥*ĈĭĝÑ$ <®Ŭò <  ƒĦ‚pƔãĄ!ZdNPm%+ƷŮ&îų:ĺ(<N_hBLl H\Pmlēš=*&+ƷŮ&îų*Ħãª×)+ĺ(! 5% <&ŧ :=<ŚĄƒĦ@‚pZdNPm+Ŵľŀ(˜ńć *9

(75) (s2!Ë£@µ$:Ʒ€-œ!ƒ£% !ČÒƱ) <Ů«-îų*Ħã+Ć:%+( 6**ƷZdNPms2!Ë£@ µ$ &+Ʒƭƛƒ*rî)¤2=$ <5ƷŮ*ĦãĚƐŀŠ 7)Æ¥$ &@ŋ¸$ <îų+à)ƒ*rî) <5Ʒƒ ĦñƔ)ŷ. ±)6Ů0')ñƔ(Ħã‚p@ŜƱ$ ( *%+(. &vô$ <=:*&:ƷZdNPm*Ů) $N_hBL lHÆ¥(!*+ƷN_hBLlHÆ¥*5)ƝŽ(ĦãÀ@ŚĄ Ɠ;Ɩ$2!$ <:%+( &ŧ !$ƷCHA @ķ ÒƱ%+Ʒîų&Ů%ĺ(<ŝĐí:=6**ƷƙúŀN_hBLlH *ľIJÆ¥<Ž»+ƷŮ&îų) $²Ė% ;ƷƏã(‚ƒĦ% < ®ŬòƲ &ŧ :=<  . .

(76)  %ƷƏã(‚Ħ=ŴƒN_hBLlH@Æ¥<®Ŭò@ēƅ ´’ƳƜŹ% <BO^jhl@ķ $ƷƒĦƈŖrđ@ƳƜ<ĂĠ )9;Çƛ:ZdNPm*ƒ@™©ƷƒĦ@ 28% 2 ćƠşû* ĂĠ)9;ƷƏã(ĦãÀ@§›)ŜƱZdNPm@„šN_hB LlH@Ɓď&>Ʒshort form )Ú$ long form Ć:)ĥÜ<˜ ńĖ*ƙúŀN_hBLlH*\Pml)Æ¥$ *ľIJ\Pml +ƷƒĦ 102%‚p±)6şû=2ƷBO^jhl@ķ ĂĠ% !$6ƷƏã(ĦãÀ@ŚĄƓ;Ɩ<9

(77) )Ĕã*‚ƒĦ2% ñ™±)+ƷşûćƠ@åƟ&$6Ʒîų«-Ů%* CIRP *N _hBLlHÆ¥(!!$ƷBO^jhl@ķ ‚ƒĦƇ Û)9!$ƷN_hBLlHÆ¥)Ú<Əã(ĦãÀ*ƝŽòèŋ¸ =CHA @ķ ‚ƒĦƇÛĠ%+ŚĄƒĦ‚p±)+Ʒîų)  $N_hBLlHÆ¥CHA @ķ ‚ƒĦƇÛĠ%+ƒĦ 32zƒ)2%‚p$:™Ÿä)ōƷÇƛ:™©$ <* 5Ʒ28zƒ@ƓƖ<5)+ř 60 ›Ŏã@ŽnĂƷBO^jhl )9<‚ƒĦƇÛĠ%+ƳƜłì:™Ÿä)$™©@Ż!$ <* 5Ʒ28.Ɨ<5)!ćƠ+ 30 ›ĊĨ% !!$Ʒ CHA @ķ <ĂĠ&BO^jhl@ķ <ĂĠ%+ƷƏã(ĦãÀ%*νć Ơ)Ƙ  !5)N_hBLlH\Pml)6Ƙ žš=&ŧ :=<  Ŵľŀ(˜ń) $Ʒƭ@ƒ*•Œ)¤3Ƒ49

(78) )ƒ@s5Ë£@& ;Ʒ8!;&ƒĦ@‚p$ ĖÌƀÙ=<Ƶ19, 31ƶ! $ƷŮ@³5tŽ(ų¹+ñī)™7=$ ( &ŧ :=<ČÒƱ)  $ƷƏã(‚ƒĦ)9;Æ¥ƙúŀN_hBLlH+ƷĔã*‚ƒĦ .ƇÛì)6˜ńĖ)Æ¥22% !*&:ƷŴľŀ(˜  . .

(79) ń) $6ƷƏã(‚ƒĦ@ƟćƠŜƱ<&)9;Ʒ*Ơ) CIRP * ƙúŀN_hBLlH@Æ¥Ʒ*ì*Ĕã*‚ƒĦć)ƍ<ƤÔ) $ <®Ŭò <  Əã(Ħã% CIRP ƚ~Ì*ƙúŀN_hBLlHÆ¥<*% =,Ʒ áàć:‚Ħ):=$ <ŗÝ) $+Ʒ˜ńć%(&6N_hB LlH\Pml˜ńć&²Ė% <®Ŭò <bCN%*Á¶Ƶ46ƶ& ²Ė)ƷLiAlZdNPm*ŗÝ*Ħã+Ʒáàć:Ųە9;6‚Ħ) şû=$ *9

(80) )‚ĦĎ}p) <ŗÝ)< CIRP ƚ~Ì*ľ IJ@ƈ/&>ƷŒ 1 ő%Ɓď@Ż!ŮƷîųƷūƷũų«-ůų&+ĺ (;Ʒáàć:˜ńĖ*N_hBLlH\Pml@ŋ$ <&Ć: &(!²n‰ƒ% !$6ų¹)9!$N_hBLlH*\Pmlĺ( !$ &:Ʒ˜ńĖ* CIRP *ƙúŀN_hBLlH*ƈŖ)&!$Ʒ Əã(‚Ħ*6*ƝŽ(Ž»% <&@ŋ$ <  :)Ʒ]T7bCN*ŗÝ%+ CIRP Pl\GƌĖ ( RNA *ÏÑò@ Ʋ5<ęŬ@ľýƷŗÌéö)Öq$ <Ƶ34, 59ƶ‹ĸŗÝ7ŗÝƧŭ ļœ*Ļó%+ŗÝĦã 33zƒ9;6oą<=:*Ļó%ŗÌéö )ĺà@*+ƷCIRP ƚ~Ì*ľIJ‚p<5% <&ijƁ=$ <Ƶ34ƶLiAlZdNPm) $6ŗÝĦã+‚ˆ=$;Ʒá àć: CIRP Pl\GƌŗÝ) $ęŬ$ <®Ŭò+§›)ŧ : =<"2;ƷCIRP *ƙúŀN_hBLlH*\Pml˜ńĖ% <& ƷCIRP ęŬ<5)ƝŽ% <®Ŭòŋ¸=à)‚Ħ% < ŗÝ) $ƷƙúŀN_hBLlH*ƈŖęĕͽ<*'

(81) +ÉÆ Ŷ·Ĥ İ)Ʒ‹ĸŗÝ7ŗÝƧŭļ*9

(82) (Ļó) $ƷČʼnŏ%ēš = long form *ľIJƲ2!$ <(:,Ʒlong form *ľIJ7ęŬ@÷ž <&ƷŗÝ)<Ļó*ā(ĞĽĠ)Ţ<®Ŭò <  . .

(83) Œ 2 ő%+Ʒ“$*ÒƱ) $ƷƏã*‚Ħ&

(84) Ď}p%+ CIRP *ƙ úŀN_hBLlH˜ńĖ% <&@ŋ!$ƷCIRP *ƙú ŀN_hBLlH@˜ńĖ)Æ¥<t(Ž»+Əã(‚Ħ% <&ŝƉ# LiAlZdNPm+ƷŴľŀ(˜ńć) $Ʒ˜ńےć*8! ;&ƒĦ‚p*Ơ)Əã(‚ƒĦ@ƟćƠŜƱƷCIRP *ľIJĖæ@Æ ¥$ <&ŧ :=<.  . .

(85) ı 3į. Ə•ĨŸĘ/A CIRP 0•ĨąŽêĥSelHRqO0ŨÍ.  þĪĭ0ùļĥ.ĦĆ1ƚ•ĨŸĘ0<( ēʼnåE•Ĩ.ŸĘ<©; $®xŸĘŗ/}wƚ †ē0›ĖEĐĢA,+Aı 2 į+1ƚ •ĨŸĘ+ARmFq`jSVrEĢ*ƚŕĤĥ.•Ĩ,1ěĮ$òĎ +'*<ƚŴÕ. †ēEŃëA,/>'*ƚ•Ĩą CIRP 0ŽêĥSe lHRqOEØů,+$0ĩš1ƚ•Ĩ.ŸĘ/* <•ĨŸĘ,¨ą0 CIRP žƒÃĤĞEŨÍ+A¥ŏåEī¯*AƏ• ĨŸĘ/*< CIRP 0ŽêĥSelHRqOEūĴAkK]TjŶ A.?2ƚCIRP 0ĉŏ/>@ûÜBA ēʼnå0žāEƚƏ•ĨŸĘ/} w+A¥ŏåA"+ı 3 į+1ƚ•Ĩ.ŸĘ,*hIS¤4 lWZEĢƚCIRP 0ŽêĥSelHRqO0kK]Tj•ĨŸĘµú0< 0.0ƚƏ•ĨŸĘ+'*<ŶA<0.0-Eö?/A ,EĦĥ,$  RmFq`jSVr/AŽêĥSelHRqOūĴ0ęà1ƚÔ҆ē ÷/Şð0SelHRqOamFqZŶA,ƚ8$ƚ"B?0Şð0 SelHRqOamFqZƚŴÕ. †ē/>'*ƚCIRP EPr[A3, (0 mRNA /ƍķA,+A"+ƚ8. 1Ə•ĨŸĘ/*ƚŞð. 0SelHRqOamFqZÔÒ÷/ĤĞ*A0-Ăť$ł *ƚƏ•ĨŸĘ/*<ŴÕ. †ē/>'*ŽêĥSelHRqO0¿  ØůBA0-EĂť$. . .

(86) Ŀā. Ô҆ēŇ0hIS/A CIRP mRNA ţĀ  Ô҆ēŇ0hIS0ŒƚáŔƚōƚŊŔƚœŔ¤4ĵÏ/*ƚCIRP ž ƒÃĤĞ0ĂĹE RT-PCR Ď/>@Ŝ'$ŒƚáŔƚōƚŊŔ¤4œŔ? 1 1 Ľ0elHhr?ķ 500 bpƚ700 bpƚ800 bp 0 3 (0QHT0 PCR ġĘ ¾ÓB$Ƙ´ 21ƙRmFq`jSVrEĢ$ÈƖ,¨ą/ƚķ 500 bp 0ġĘE short formƚķ 800 bp 0ġĘE long form ,$8$ƚĵÏ?1ķ 500 bp 0 short form ×Bƚ|0 2 (0QHT0 PCR ġĘ1 short form ,ċ ŵ*ö?/ŚŽ,*×B$Ƙ´ 21ƙ   áŔ?¾ÓB$ PCR ġĘEĢ*ƚRrNJqSţĀ/>@¼¹ƁšE ţŪ$Ŀā,*ƚshort form 0Ɓš1ƚYrVfrS/º¬0AhIS 0 CIRP mRNAƘNM_007705.3ƙ,ǐ/sŖ*$Ƙ´ 22ƙ8$ƚlong form 0Ɓš1ƚYrVfrS/º¬0Aų”amFqZƘXM_006513165.1ƙ,s Ŗ*$yçBA ORF 1ƚshort form , ORF 0ƇÂĖ? 502 bp 8+1 ǐ/sŖ*$sòƚ3’ ýİŒ0Ɓš1ģ.'*$Long form 0Ɓš 1ƚshort form 0ùÝ0JMUq0 5’ ýİŒ/SZWeP[qE©: 217 bp 0 ¼¹ƁšìB$<0+'$Ƙ´ 22ƙ. hIS/AŴÕ. †ē/>A CIRP mRNA 0SelHRqOĤĞ5 0ÛƑ  ()*ƚ`jSVr+ CIRP 0ŽêĥSelHRqOE¿ !$ŴÕ. †ēhIS/*< CIRP žƒÃEūĴA¥ŏåEĂť$ĠÅ0$; /ƄŠ.Ŕ°,*ƚŒ,áŔ/ŀ'*ţĀEŜ'$Ô҆ēE«*$ hISEƚƗƂ,–£0Ľ9§D!+†ēE 28 5ŨÍƚ1 ÷ƈŃë$. . .

(87) RTPCR 0Ŀāƚ†ēE 28 +Ńë$Ň+1ƚáŔ¤4Œ/*ƚSel HRqOamFqZ short form 5ƍķ$ƚ•ĨŇ,Ɠ„0bVrq× B$Ƙ´ 23ƙ  8$ƚhIS0†ēEŴÕ. ē¸/Ĕ¶!. ƚäŷ/ 15 8+ u!. $»§/1 CIRP 0SelHRqO/ÛƑEwA-EĂť$hIS /HUcnlqEª!$Ýƚŷ=/ 4 /Ńë$–řÖ/ĬŸ!$ 0òĎ/>@ƚ32-28 0ēÕ¸/Ĕ¶A÷ƈ1 5-7 ˜%'$67-72 ˜Ƙ69

(88) 2 ˜, n=3ƙÝ/hIS0đƀ†ē 15 /Ż$CIRP 0SelHRqOb VrqEţĀ$,Cƚ†ēE 15 8+ä–$Ň+1ƚáŔ¤4Œ/ *ƚshort form 50ƍķ1ö?/ŧ;?B.'$Ƙ´ 24ƙ  ?/ƚHUcnlqEªÝƚ†ēE 37 /Ńë$Ň+1ƚáŔ¤4Œ /*ƚŽêĥSelHRqO0bVrq0¿ 1ØůB.'$Ƙ´ 25ƙ  8$ƚŒ¤4áŔ/*ƚshort form , long form 0ĤĞƅ0ċEij—ƚ Ô҆ēŇ0`jSVr,ċŵ$,Cƚ†ēE 28 +Ńë$Ň+1úè .Ñŧ;?B$Ƙ´ 26ƙsòƚ†ēE 15 8+ä–$Ҥ4†ēE 37 /Ńë$Ň+1Ô҆ēŇ,ċŵƚŁŤÆĥ/úè/¿ *A,1 ŧ;?B.'$Ƙ´ 26ƙ. h IS/Aʖğ½+0ƔŌ/>A CIRP mRNA 0SelHRqO ĤĞ50ÛƑ  †ŝƐ?0ʖĕ/>@ CIRP 0ŽêĥSelHRqOūĴ¿ A 0-EĂť$"0$; 4 0ğ½+ 1 ŹƈƔŌ$ʖğ½ƔŌŇ0 hISE‡— CIRP mRNA 0ţĀEŜ'$RT-PCR 0ĿāƚhIS0Œ¤4 áŔ? 3 þ0aq[×B$Ƙ´ 27ƙ8$ƚshort form , long form 0Ĥ. .  .

(89) Ğƅ0ċEij—$ʖğ½ƔŌŇ,Ô҆ēŇ0hIS/*ƚúè. Ñ1ŧ;?B.'$Ƙ´ 28ƙ. Ô Ò†ēŇ0lWZ/A CIRP mRNA ţĀ  Ô҆ēŇ0lWZ0ŒƚáŔƚōƚŊŔƚœŔ¤4ĵÏ/*ƚCIRP ž ƒÃĤĞ0ĂĹE RT-PCR Ď/>@Ŝ'$áŔƚōƚŊŔ¤4œŔ?1 1 Ľ0elHhr?ķ 500 bpƚ700 bpƚ800 bp 0 3 (0QHT0 PCR ġƾ ÓB$Ƙ´ 29ƙRmFq`jSVrEĢ$ÈƖ,¨ą/ƚķ 500 bp 0ġ ĘE short formƚķ 800 bp 0ġĘE long form ,$8$ƚĵÏ?1ķ 500 bp 0 short form ×Bƚ|0 2 (0QHT0 PCR ġĘ1 short form ,ċŵ* ö?/ŚŽ,*×B$Ƙ´ 29ƙ?/ƚŒ?1ƚķ 1.1kbp 0 PCR ġĘ×B$8$ƚŒ/*ķ 500 bp 0 short form 1¢˜/×B $<00ƚķ 700 bp ¤4ķ 800 bp 0 PCR ġĘ1 short form ,ċŵ*ö?/ ŚŽ,*×B$Ƙ´ 29ƙ. lWZ/AŴÕ. †ē/>A CIRP mRNA 0SelHRqOĤĞ5 0ÛƑ  ()*ƚ`jSVr¤4hIS+ CIRP 0ŽêĥSelHRqOE¿ ! $ŴÕ. †ēlWZ/*< CIRP žƒÃEūĴA¥ŏåEĂť$ 22 +ƔŌ*$lWZEƚƗƂ,–£0Ľ9§D!+zėĥ/ 28 0 † ē+ 1 ÷ƈŃë$RTPCR 0Ŀāƚ†ēE 28 +Ńë$Ň+1ƚáŔ¤ 4Œ/*ƚSelHRqOamFqZ short form 5ƍķ$ƚ•Ĩ÷,Ɠ „0bVrq×B$Ƙ´ 30ƙ. .  .

(90) ´ 21ƜÔ҆ēŇ0hIS/A CIRP mRNA ĤĞţĀ. Ô҆ēŇ0hIS/*ŒƚáŔƚōƚŊŔƚœŔ¤4ĵÏ/ĤĞ* $ CIRP mRNA 0 RT-PCR ġĘ0ƎČEīPCR 1* 30 QHNn+ Ŝ'$”ħĈ0ð‹1hrKr0 bp Eīķ 500 bp 0ġĘE short formƚ ķ 800 bp 0ġĘE long form ,$~ŝ,* 3 ¡0hIS/AĿāEī .  .

(91) ´ 22ƜhIS/A short form ¤4 long form 0JMUqĄŸ. Short form ¤4 long form 0JMUqĄŸEć×´+ī1ƇÂP[qƚ 1ļĊP[qEīðÄ1ƇÂP[q?0¼¹ðEīOor0±Ţ+ ī$ƀ˜ƚlong form 0& short form ,ģ.AƁš+A. .

(92) ´ 23Ɯ†ēE 28 +Ńë$Ň0hIS/A CIRP mRNA ĤĞ. †ēE 28 +Ńë$hIS/*Œ¤4áŔ/ĤĞ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČEīPCR 1* 30 QHNn+Ŝ'$”ħĈ 0ð‹1hrKr0 bp Eī~ŝ,* 3 ¡hIS/AĿāEīÐ /ċŵÌŭ,*Ô҆ēŇ0 PCR ŽEī. .

(93) ´ 24Ɯ†ēE 15 8+ä–$Ň0hIS/A CIRP mRNA ĤĞţĀ. †ēE 15 8+ä–$Ň0hIS/*Œ¤4áŔ/ĤĞ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČEīPCR 1* 30 QHNn+Ŝ'$ ”ħĈ0ð‹1hrKr0 bp Eī~ŝ,* 3 ¡0ĿāEīÐ/ċŵ Ìŭ,*Ô҆ēŇ0 PCR ŽEī. .

(94) ´ 25ƜHUcnlqEªÝƚ†ēE 37 /Ńë$Ň0hIS/A CIRP mRNA ĤĞ. HUcnlqEªÝƚ†ēE 37 /Ńë$hIS/AŒ¤4áŔ/Ĥ Ğ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČEīPCR 1* 30 Q HNn+Ŝ'$”ħĈ0ð‹1hrKr0 bp Eī~ŝ,* 3 ¡0Ŀā Eī ċŵÌŭ,*Ô҆ēŇ,†ēE 28 +Ńë$Ň0 PCR ŽEī. .

(95) ´ 26ƜhIS/AHUcnlqƗƂ/>A CIRP SelHRqOamFqZ0¿ . ¦Š†0Œ¤4áŔ/A short form , long form 0ĤĞƅ0ċƘlong form/short formƙEij—$Ô҆ēŇƘn=5ƙƚ†ēE 28 +Ńë$ŇƘn=4ƙƚ †ēE 15 8+ä–$ŇƘn=3ƙ¤4HUcnlqEªÝƚ†ēE 37 / Ńë$ŇƘn=4ƙEÔ·‹

(96) SD +īÁƄċŵ/1 Dunnett’s ĎEĢƚ Ô҆ēŇ,"B#B0Ň0‹Eċŵ$**1Ô҆ēŇ,ċŵ*ŁŤĥ /úè+'$,EŝƘP < 0.01ƙ. .

(97) ´ 27Ɯʖğ½ƔŌŇ0hIS/A CIRP mRNA ĤĞţĀ. ʖğ½ƔŌŇ0hIS/*ƚŒ¤4áŔ/ĤĞ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČEīPCR 1* 30 QHNn+Ŝ'$”ħĈ0 ð‹1hrKr0 bp Eī~ŝ,* 3 ¡0ĿāEīċŵÌŭ,*Ô Ò†ēŇ,†ēE 28 +Ńë$Ň0 PCR ŽEī. .

(98) ´ 28Ɯʖğ½ƔŌŇ0hIS/A CIRP SelHRqOamFqZ0¿ . ¦Š†0Œ¤4áŔ/A short form , long form 0ĤĞƅ0ċƘlong form/short formƙEij—$Ô҆ēŇƘn=5ƙ ƚ †ēŇƘn=4ƙ¤4ʖğ ½ƔŌŇƘn=5ƙEÔ·‹

(99) SD +īÁƄċŵ/1 Dunnett’s ĎEĢƚÔ Ò†ēŇ,"B#B0Ň0‹Eċŵ$**1Ô҆ēŇ,ċŵ*ŁŤĥ/ úè+'$,EŝƘP < 0.01ƙ . .

(100) ´ 29ƜÔ҆ēŇ0lWZ/A CIRP mRNA ĤĞ. Ô҆ēŇ0lWZ0ŒƚáŔƚōƚŊŔƚœŔ¤4ĵÏ/ĤĞ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČEīPCR 1* 30 QHNn+Ŝ'$ 2 ¡0lWZ/AĿāEī”ħĈ0ð‹1hrKr0 bp Eīķ 500 bp 0ġĘE short formƚķ 800 bp 0ġĘE long form ,$.  .

(101) ´ 30Ɯ†ēE 28 +Ńë$Ň0lWZ/A CIRP mRNA ĤĞţĀ. †ēE 28 +Ńë$lWZ/*ƚŒ¤4áŔ/ĤĞ*$ CIRP mRNA 0 RT-PCR ġĘ0ƎČEīPCR 1* 30 QHNn+Ŝ'$”ħĈ 0ð‹1hrKr0 bp Eī~ŝ,* 3 ¡0ĿāEīċŵÌŭ,* Ô҆ēŇ0 PCR ŽEī.  .

(102) ňË.  ı 3 į+1ƚ•ĨŸĘ+ARmFq`jSVr/*Ŷ$ CIRP žƒ Ã0ŽêĥSelHRqO0ūĴkK]TjƏ•ĨŸĘ/<ŶA0Ăť$"0ĿāƚƏ•ĨŸĘ+AhIS¤4lWZ/*<ÔÒ †ē÷/1ĉŏĥ. CIRP mRNA E©:Şð0SelHRqOamFqZÅ ¶A,ö?,.'$8$ƚ`jSVr,¨ą/ƚŴÕ. †ēƚ CIRP 0ŽêĥSelHRqO0bVrqE¿ !A,<īB$  þĪĭ/*ƚhIS¤4lWZ/*<ƚÔÒ÷? CIRP mRNA E© :Şð0SelHRqOamFqZ0ĤĞŧ;?B$0,?ƚhI S¤4lWZ+<ƚ•ĨŸĘ,¨ą/ƚŽêĥSelHRqOE™@øA0 9+ĉŏĥ. CIRP mRNA 0ĤĞ0žĝE¾=,+A¥ŏåAh IS/A long form 0Ɓš1ƚYrVfrS0<0,sŖ*$<00ü %ĉŏ/(*1º¬B*.<0+'$Ɓš?yçBA long form 0Pr[AVqbNŮ0ĄŸ1ƚĉŏĥ. CIRP 0 C ýİ0 5 (0Fi _ƃƚģ.A 9 (0Fi_ƃƁš/ņíD'$Ú+'$0Ɓš0Ą Ÿ1ƚı 1 į+ö?,.'$RmFq`jSVr0 long form 0ĄŸ,¨ą+ A$'*ƚÔ҆ē÷0RmFq`jSVr,¨ą/ƚƏ•ĨŸĘ+ AhIS¤4lWZ/*<ƚlong form Pr[AVqbNŮ1ĉŏĥ . CIRP VqbNŮ0[i\qZ^LXGd,*0ĉŏEë(¥ŏåA (8@ƚŽêĥSelHRqOE™@øA,/>@ƚCIRP VqbNŮ0Ĥ ОĝEtõ!A%+.ƚĉŏE¢˜/Ĥî!AkK]TjhIS ¤4lWZ/*<D'*A,ī¯B$  ı 3 į+ù<ƄŠ.Ŀā1ƚ•Ĩ.hIS¤4lWZ/*<ƚzė ĥ/ †ē/ŨÍA,/>'*ƚ•Ĩą0SelHRqObVrq50¿. .  .

(103)  E“Ğ+$,+Azėĥ. †ēŨÍ1ƗƂśEĢ*ŜDB$ ƚƗƂśEª!$Ý/ 37 +Ńë$»§/1SelHRqO1¿  .'$$;ƚSelHRqObVrq0¿ 1ƗƂś0ÛƑ+1.ƚ †ē0 u/ˆÅA<0,A8$ƚhIS/*1ƚäĕ/†ēE u!$»§/1 CIRP 0SelHRqObVrq1¿ .'$$;ƚŴ Õ. †ēŽêĥSelHRqOE¿ !A$;/ƄŠ+A,EÙ ïëAĿā,.'$ŽêĥSelHRqOŴÕ.ēÕ¸+ØůB A,1ƚRmFq`jSVr,¨ą+A$'*ƚ ēEĕ,* CIRP EūĴAkK]Tj•ĨŸĘµú0<0+1.ƚƏ•ĨŸĘ/<‘ Ŷ*Ŷ*A¥ŏåA,ī¯B$0ň,sŖ*ƚh IS+1ƕ0vŰ,ğ½ēÕ0 u/>'*ƚ1 ó’+†ēE 10 6- u !Aó’‚ĨEŜŸE,A,ĩ?B*AƘ3ƙ8$ƚlWZ/ *<ƕ0vŰ/>@ƚ1 ’+1A<00đƀ†ēE u!AĉĄ D'*AƘ42ƙ$'*ƚŲ+†ē uAĚč1ƚ•Ĩ0>.ăÕ 0 †ē+.*<ŶA"0$;ƚ•Ĩ.ŸĘ/*<ƚ ē/ >A CIRP 0ĤĞĉĄ‰ÅB*A¥ŏåA  †ŝƐ?0 ēĕŽêĥSelHRqOE¿ !A-EĂť A$;ƚhISEʖğ½u/* 1 ŹƈƔŌ$1 Źƈ0 ēğ½ƔŌ, ÿ1ƚhIS/*şŘŐŋĽŅ0źÚé/~ŝBAʖ50ƒ  ŧ;?BAÿ+AƘ47ƙ(8@ƚ0ÿ+1ƚ†ŝƐ?0 ēĕ1 ¢˜/Ų/ÛƑE¤7*A,ň?BAʖğ½ƔŌ09+1ƚ ŽêĥSelHRqO0bVrq/úè.¿ 1.'$$'*ƚCIRP 0SelHRqObVrq1†ŝƐ? ēĕ+1.ƚĽŅ"0<0 ē,.AâŠA,ö?,.'$ÈƋ/ƚĽŅ0ēÕđƀ†ē>@ ĵÏ+1ƚÔ҆ē÷/*< long form Śƚshort form ÙĤĞ. . .

(104) *$"0$;ƚŴÕ. ē"0<0ŽêĥSelHRqO/ÛƑA, €ũEïëAĿā,.'$  lWZ0Œ+1ƚlong form 1ƚÔ҆ē÷/<ĤĞÎ.<00ƚŴÕ. †ē/>@ long form 0ĤĞ1>@ĒÎ*A,<ö?,.'$$ '*ƚŒ/*<áŔ,¨ą0ŽêĥSelHRqO0ūĴ/>A CIRP ž ƒÃœßĉĄ1ŶA,ň?BAsòƚlWZ0Œ?1RmFq`j SVr=hIS?1×B.'$ķ 1.1kbp 0aq[×B$8$ƚ ŴÕ. ē/*<ķ 1.1kbp 0aq[1¿ E.'${Ýƚķ 1.1kbp 0 PCR ġĘ0ƁšEţĀAâŠA<00ƚlWZ0Œ/Ɖ*1ƚRm Fq`jSVr=hIS,1ģ.AkK]TjŶA¥ŏåA  ıƛį+1•ĨŸĘ/*ƚCIRP žƒÃ0ĤĞūĴƚ †ē/ƊAƋ/ ĸô CIRP VqbNŮ0ĉŏEĤîA$;/ƄŠ+A,ňË*$s òƚþį/*ƚ•Ĩ.ŸĘ+AhIS¤4lWZ/*<¨ą0 ŽêĥSelHRqOĤĞūĴŶA,ŧ;?B$0,?ƚ B?0ŸĘ+1•Ĩ÷0>. †ēƌÉ?‰ŬAĦĥ,1ģ.AĦĥ 0$;/ŽêĥSelHRqOūĴEĉŏ!*A¥ŏåACIRP E_ WNFIZ$ĻŎ+1PqZprn0ĻŎ,ċŵƚmitogen-activated protein kinaseƘMAPKƙ= WntƚFgZrRSIJ0RO\nľű0ĤĞ/ö?.ż š—B*A0,?ƚCIRP 1æÒĥ/ĉŏ*A,AƘ51ƙ B?0RO\nƒŻĶ1ĻŎ0ĕ/Ì*ĸôÌãƚĠ†0æÒåE ‰'*A(8@ƚ•Ĩ÷0>.Ų †ē/ƊA,Ěču+. ,<ƚĻŎofn+1ƚŽêĥSelHRqOEĢ$ CIRP 0ĤĞtõĉĄƚ Ġ†0æÒåEŃëA$;/ĉŏ*A¥ŏåA8$ƚCIRP Vqb NŮ0ĤĞ0tõ/1ƚ ē0|/< ƃĸƚĺÀńIJ0ĕØƆ,.A Ƙ45, 55, 64, 65ƙ"0$;ƚ †ēÀ0г/>'*ƚCIRP 0ŽêĥSel. . .

(105) HRqOūĴØůBƚæÒåE‰($;/ĉŏA¥ŏå<A{ ÝƚCIRP 0ŽêĥSelHRqO0 ēÀ0г,žā/(*<ö?/ *,ûÜBA  þĪĭ+1ƚRmFq`jSVr1ƚCIRP 0SelHRqOœß/>@ † ē/ÌAʼnåEĜÞ+*A¥ŏåA,ň*Aþį0Īĭ/> '*ƚ•Ĩ.ŸĘ+AhIS,lWZ/*< CIRP 0ŽêĥSelH RqOE•Ĩą/œß+A,ö?,.'$$'*ƚƏ•ĨŸĘ +'*< †ē/ÌAʼnåEĜÞ+A¥ŏåī¯B$•Ĩ. ŸĘEٜĥ/ †ē,A,/>@ƚę/Ŗ­ĥ.ƌÉ1ƚáŔ/A vñő+AƘ13ƙ"+ƚ•Ĩą0 CIRP ĤĞą×088 †ē/͏A ,/>@ƚáŔ/ůA †ē÷0ƌÉE²ſA,ûÜ+A{Ýƚ Ə•ĨŸĘ0 †ē÷0ƌÉ/Ì*-0>.žāEĤîA0-Ŧ Ļ/ţĀ*âŠA. . .

(106) š ¡ŦÈ.  ďŇŏ&Ƹ‰Ń˜Į%0{|Īŧê8Ƭ‰Ń˜Į$uj5! $25ׯ8­Ɨ5!8ĉŝŀ#łĚ!5. {|Ī. Ƹ{Īi. Ŀĵ. 8hĄƸ3$ŜŨ%€Ƈěũ8Ċ5{ĪD_JAIeSAƊ CIRP $ ńłŔ 1 ő. &‰Ń˜Į. 5Da:eQ\FIf$ 5‰ŃĆ%. CIRP %ƕóŀFW`<DeB%¸™8ą3 !Ŕ 2 ő W`<DeBƂŕ%¸™8Ù

(107) ƋŶ® ƍÖ%{|Ī ŹąŔ 3 ő. &%ƕóŀF. 5!$. &Ƭ‰Ń˜Į$ ‰Ńę% CIRP %ƕóŀFW`<D. eB%Ƃŕ8‡ĵ5!$ð–%!$24Ƹ‰Ńę% CIRP %ƕ óŀFW`<DeB%Ƃŕ8sƸƬ‰Ń˜Į$0{|Īŧê8uj.

(108) 5.  ũê ʼn©6ƶ° 31Ʒ  ĕĮ$ &ƸĪÖ$24ƕóŀFW`<DeB ¸™5!. %´¥ 4ƶ37, 53ƷƸ%]?OG\ ¨m˜Į$0 Ɛ5 ũê 53$Ƹ¨m˜Į%³ƲŜŨ8ĺŇŏ$2Ƹ{γƲ$24 CIRP %Ŀĵ%hĄ!!0$ƸƕóŀFW`<DeB$ƞŶ#®¾. 5 SRSF5. %Ŀĵ hĄ5!´¥65ƶ15Ʒ Ƹĸ|†. 0 SRSF5 %. ¸™8s CIRP %ƕóŀFW`<DeB ¸™ ũê 5!Ŧ 5,4ƸƍÖ#{Ī$24ƕóŀFW`<DeB ¸™5!Ŀŷ &Ƹ¨m˜Į$ 5ƕóŀFW`<DeB8sĪÖ$25IeSAƊĿ ĵ%ƂŕěĘ8ĸ|†  6,. Ƹ{Īi. lŶ#Ŷ®. ŷŒƞŶ#Ņŷ. 5. % CIRP IeSAƊ%hĄ$&ƸƌˆcXb%Ħê™. 5!Ŧ36

(109) Z=F%ŢŠůŜŨ8ĺŇŏ$ . ƸƍÖ#{Ī&ƕóŀWd^fIf8¸™5!$24Ɩy¾%ƌˆ8 Ħê™5!´¥ 5ƶ1Ʒ,Ƹƌˆ®¾ Sp1 32%ĪÖĐxi. 73.

(110) $ Ħê™ƸCIRP mRNA %hĄ$Çj5ƶ49ƷgāƸƌˆà Ƃŕ$25ěũŀ# CIRP %hĄ$0´¥ 5!Ƹ{Ī&Z =F%ŢŠůŜŨ$ & CIRP % pre-mRNA 3%FW`<DeB—Ĵ8 hĄ5ƶ17ƷďŇŏ. ą3 !ƕóŀFW`<DeB%¸™&Ƹ¨. m˜Į% CIRP %Ŀĵ]?OG\$ ĀŸ%Ŀŷ!#  ‰Ń˜Į8ĺƸ‰ŃĆ%¸™$ą3 $5!8łŀ!  %Ňŏ Ŵ765%k%(!$ƸDZaF8ĺ Hibernation proteinƶHPƷ%Ňŏ 5ƶ24ƷDZaF hĄƸūŋŞř. ƞŶ#ݔ8ô5%. &Ƹ‰ŃĆ$%-ū†. HP . &# !´¥65. 63%Ňŏ$ĺ365DZaF1ƸEaF#"%‰Ń˜Į&Àŕê% ‰Ń8Ŵ Ƹ63%˜Į8ĺá36¸™$&Àŕê%® ¾ ƣj5 ũê8Ŧî5æŶ 5ÄƩ$ƸHP %Ŀĵ$&Ô¦ aG\ƶcircannual rhythmƷ ƣj5ƶ24ƷgāƸďŇŏ Da:eQ\FIf&ƸÄƳņ. ÄƳ$ĺ. {Ī)ąĈķ¶8ĈČ ơ#52. $Ƃŕ5!$24 1 Ô8ƐŭĿŀ#‰Ń8Ŵ!.

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