ガ類性フェロモン産生の分子機構に関する生物有機化学的研究（PDF）

全文

(1)s»:¼»;½. c <¼½». s3. .

(2) !. . .

(3) .

(4)

(5) . HD C1=02 !PQRAT)*k. !"#$% &'( )

(6) *. =`1[?34 Y 89: p50 !PQR. +,

(7) -./01'234 / . AT, cDNA 9$F#1.\2. 5 Butenandt6789:1;< =. 18Q*/0)!PQRAT EST (Expressed Se-. !"2 20 #1$>23 1959 #%? < &-'(. quence Tag) h#o#

(8) 1)234 . EST vQ#A. 02@AB:#% )*1 234 Butenandt 0. Q!#%,9 !PQRAT5Tk1. Karlson / + @AB:#% ,C , -.. 234 / k i#vN 2 . EFGH0 pherein 6IJ7 0 hor-. @AB:#%/w\k pgdesat1 , pgFAR . */D&'(1. 2000 vQ#A. mon 6K1.L7 MNO) pheromone 6!PQRA7 02. C\234 pgdesat1 fg{

(9) ). 3234 <S4 5TUN/D&'()V!P. g{ 6%tA{7 2 _)¡yz¢A1u*. QRA = /W/6K70X8Y. bifunctional N

(10) )V 1. Z 9 : /':134 5 . £b 9N!PQRAT5T multifunc-. &!PQRA ;N<[\= 6 >. tional

(11) !"tF# . ]7 )V/0 \?@^ _`A. /Y). o pgFAR v'%?C.3fg{G%¤¥. 500 SB &!PQRAC.;4 2. ) 1£b ¦p §¨

(12) f©. 2 / 5TN&!PQRAa ,CND. uv

(13) /\fg{G%¤¥C.. EM a ,CNFGb)0bHD 8

(14) c#d1e. 34 Y3 pgFAR ) bH=`; . I/. 0;CJNKL f=.;N. k1u23ª(m«89:\1-23/0. ;4. !PQRA/w\k)ª(m«23. /Y)M !PQRAN;5ONg71X.

(15) f. 0NZ34 . T/',\ 6¬v7 0;C_ ). 89:1Rh%02 &!PQRAI/ D8i. H&1X¡*/00NZ34. jk PQ = 1=R234 < lS) !PQRA. 3.. PBAN 89&4567' :; RNAi <. TUV)mWX@AB:#%I/Y3.YnYN9. =>. oApqZr9stuv[*/01w;x2. &!PQRA I/;¢ y1X PBAN. / lS1!PQRyzP{G

(16) 03500X -|. sb (PBANR) k. 9oAp \M1D}o%)=. £¤9§#1;3 RT-PCR. */01]~. ,z 2 PBAN sb®¥ /D&¯d)V. 34 <2 ^b / D/' .YnYN". i°#QzA U sb!"tF#¦* 7 ±§²¨³. 1_23=`1a[?/0) < b1 . G Av(w³sb (GPCR) )V/01¡234 .. 234 S MZ3 N\ 1*4. kbH 3N=`"02 89:-1;. 1. "#$%&'()*. 3 in vivo ) RNA ´ © 1 f 2 / o 1 ; . 89: !PQRyzP{G

(17) ) m 12 cW,. PBAN sb1µ?3 5 !PQRAT5Tk. UV(^ deNfgh ij/00X .YnY. ( pgdesat1, pgACBP, mgACBP, pgFAR, PBANR) @AB:#. N!PQRAT5Tk lNmnD&/4 . %I/lSy1 in vivo )ª234. o. mXpe%RA PBAN (Pheromone Biosynthesis. Activating Neuropeptide) qr1!PQRATsb. *0. UV Ca2 UVt u. fgh D= 6FFG. PBAN ?@ABCDEFGHIGJKL.M. 4.. PBAN r « !PQRAT ¬); . ;XUV Ca2 ®¯)V3?. PBAN GsF.

(18) 7 v2[2 @AB:#%w\.4 Y !. AUV Ca2 u1°CX 0±¶;3. PQRATUV5xNfgh y1 f*3? <. / ²³ª .;NZ34 @AB. )\\D1z W)*=`23 fgh ;\D. :#%I/UV·´ Ca2 9#zA1;. @AB:#%W1;N)\fg{0*.YnYND. PBAN r°CUV Ca2 v 1%?µ¸0¶. NpFG%FQ#%)V fgh PBAN. /0\234 . / D/' ¶. r_|2@AB:#%W1}~*&!PQRAW. "1; PBAN sb1¹23Gs%k·1=`. 02bH2;/01 234. 2. 2. +,-./01234567. 1,4,5-pF

(19) FA{ (IP3) 1¹238yi8%e,. @AB:#%I/ DE0</Av&b. PBAN r. ¸³ G Av( Gq, 9yGp#% ¹V. Ca2

(20) p ºº_|2D&.

(21) p.v&.

(22) 4. ¸HJ¹eyº». ¼½7. ῐ 1 BC,6 !"#1278 Moto et al.: Proc. Natl. Acad. Sci. USA, 100, 9156ῌ9161 (2003); Hull et al.: J. Biol. Chem., 279, 51500ῌ51507 (2004); Moto et al.: Proc. Natl. Acad. Sci. USA, 101, 8631ῌ8636 (2004); Ohnishi et al.: Proc. Natl. Acad. Sci. USA, 103, 4398ῌ4403 (2006) ; Hull et al., Biosci. Biotechnol. Biochem., 71, 1993ῌ2001 (2007), ·. Ca2

(23) . ;%&'<=DB%wm%&'d#& GSLxy/t.

(24) . z 674{-|M L/ df} %_Ddf. !. d%_ *Nc:;O<~Dc:;PQt;u/RS. "# )*#+,-. / 0 1 2

(25) 3 /. ##` 4 *NSy/tz 67T. 45 ).. U)PrIKV*N$O( 253

(26) *. . !"#$%&'( PBAN . 6 !"#78/9 : 1980

(27). N$OE-k` M$4: rIVL. ;< Roelofs )=. 2$/>? 1989 . WXV u/gP YZ )[? . ). /; PBAN 08@2A7 4<. !"#$R.o<2' \4I]^_. /BC,6DEF,6 G3! BC,6:. 4. 4 g`Y: uaS/ -. H !"#" #I$%#J4$K%LM. F

(28) Sb*NSy_Z/Lc. &NO 3'(2)P :*+,-QD*#+. L( Xd3G . ,-$R. (pgdesat1, pgFAR), PBAN / GST - 4 7012 U3V

(29) /W . J: e3f4gXeyhgh5L. 2X3BC,6Y/ RNAi 4: YZ12. (< Ji!/( Adrien Fonagy ). !"#"70 in vivo &5 S? [3. #6d-xyaBs-. jkX ) . lm ). 674-Q \/ ]^ :. . J¡0 )X. no )X. J. Joe Hull ) ¢C. _`a89 Ca2 (SOC channel) b PBAN. £#&. ¤p¥q rq4/:s45L. c:;%&'<=>V L/ PBAN %&.

(30)

(31) /tuv¦ )4§¥qG. '2]?$/d#@e Ad#@eb3dfd%_Da. ¨pX©wJ12rIyxªyk\z «¬. %ghi1 je 78B klm2VC. f {p: ® | y¯ M3°}. \ \n4o<2DE2FGp BC,6: qH. )P~Z4/ X;t;a79U)P. 6rI4G"srI 453t< J:uK. ~Z/±Puv¦ ]²/ y;<. jL G? . /<³G§G¨py ´. ῌ῏῎῍. µ yy¯yLx. 6 !"#1 : v"#/bc:. yx´ kῌ/P¶G .

(32)