異化性硫酸還元細菌に関する臨床細菌学的な検討 : 膣内細菌叢におけるDesulfovibrioの存在について

(1)

Title

異化性硫酸還元細菌に関する臨床細菌学的な検討 : 膣内細

菌叢におけるDesulfovibrioの存在について( 内容の要旨

(Summary) )

Author(s)

市石, 卓

Report No.(Doctoral

Degree)

博士(医科学) 連創博甲第3号

Issue Date

2010-06-30

Type

博士論文

Version

none

URL

http://hdl.handle.net/20.500.12099/33065

※この資料の著作権は、各資料の著者・学協会・出版社等に帰属します。

(2)

Lt jf. _{( -1=}

nlt.;

)

I:1).[=(;.I:a) fLrt-_ I).'il-J:i-'=(Ill:1,1.I:i--_lf;.il. I-tli':(:!!:l1.I-i-Fj

r.-j-T]I. Jj(

I:='{L:(_(/:

[;-I:i?lil !&-E]

'1it(1J_I:.i-'[Ti?_{_i-}'I1.l{J1_i

_f<{

L'_:i

i-f1--(:I- _{I;T_(;...)'i)))kL,I;1J}

t*

j=

(Bi4)

EP#

3 _%

5FBE 22 * 6 _j] 30 P

rig.fi;i.FTf

ifti_I:-}': -L!iJi

:iT(i.ILlliF,iEtY*3E;.jt3:u]I'f-;i:I Ily[:-J+ 73Eh;A.-.r-.Lf*turLi!_:i;-;'':[')I.J,jL11*..:I-.f

-BfPq#B]653i=ib'Bla

Desulfovibrio 0)rf#E=-3V

IT-(Study

on dissimilatory _{sulfate-reducing} bacteria in human

-First isolation _{of Desuuw1'bn'o} from

_{vaginal flora-)}

(_TF_-[1l'_)if*1.1. I,- i.i:- ([ll fl'-(F.,irhtf_)

.kill. Ji-: uT.T. -i- I ([.I.'ItJITE)1#il1. tI7iEih -):r',

-!DL-i;-ITi71-[),IJiiV) iRT-a

[dfqt5%tE]Bq]

% E]

_{Oy7)i+3tg&*BqS;'L(=J=L)}

BJ]6btttJ?f=4iBiPq

_{0)a##,LO) S#1i(=Bg18G#RfRAl*.}

Fq

EE)#@5%{jE

a)3E4TR4&l#tL-C. 1iAO)f#fR#i)(T7iEfO)

SJJ>+9iBiTiO)5&55 (=%b6f.

fBi(=%5%L&L

lfJ:b{64t#

tLTliL5tif3e1$1%tL15%ihf3tfSLTL1%.

Pq

EE)124k&#@!AFjEbt6

lihRt5r[i2@b(75&F5E(=L7]iA#S

^%.

3e##b{i-E.&%b{.

i%j51&rf BqS;'itlif#6i

(=<L1###

G19tO)gRt5r[i*#0)rfiELfn

6hTL 18 o

DesuuoTq'bn'o (i. PTOteObacten'a Pq _{6 -Pmteobacter1'a}

mO)j%1ETiqL&iZ5ta#TC>&

L).

##50)1t%dl

rE4&-CL&%i&P7K#h.Rfuj7]

LTfE,i?.1%*5T>.

rfTii(iJJ>tj:Llt%i6^TLt%.

iE*. =0) DesuuoTq'bn'o

b{Bg$1%

Fq

EE)i2Rg5%rEO)rEmd*%b{iehn1%1@6]

(=& i).

4S(=A;7iR85BrEO)FjEm4b{5L1=

tbt6.

El5B:1if*J:a#tLT;'i

E] S^TL1%. LblLJ=b{6. DesuMoTdbn'o

_{b{Bgji1%PqB]iiR8%rEO)#}

W.

5%45tift%i6h%##0)4i45Ab160y7)LAB4X;'5tP%h60)tE#iE@5aTi1#R.

*##0)*#jF,i

(=3S(+%iHRB3(=Bg1%1SBf15d)TZLL1.

iE*. DesuuoTq'brjo

_0)5Fi'1E%

L

EjB2ha#?A(=aSf+%ihFZB7(=E!R

L-C (i

tt(=liii9tFEEiSgfr5tO)Bg5ii2b(5RP&Sh-CL1%=tth

i).5%3e*j:G#*jfLbS>JSET#qS^TL1%b{. %^ 6 J215tO)%F!W(=?LITfif2tAJt+4*;JShTL 1*j:L1

o &*Je=5rL%i5b{Bgb% Pq EE)i*Rg*4TEO) i+=%%T.h%*#

#o)B%Ffga#jSf=3S(+%g=HRB3(=BgLTfi.

L7]i+4:gaqaBq$5i(=ct?TL.

*##i){@#a#tLTfitHIjaLJg

L_{1=tbSIJ] 6btt*j:?TL} _t%0)aft..

_{IjaBS,#.-C1*%0)rfTiSiTtBBTLb8.}

EF

;#%(i. 5E#*Bq

(=DesuuoTq'bn'o t5i%1%=ti)Shin 6^TL

_{1%&RJt5rL12$9>jEfEa5b{BiPqa5?A(=571}

iEL1=tO)#%b{&%=t(=;'i

a LJ' DesuuoT7'brlo

_fiB2Pqa5jE*

_{f=Tfri18qgElib(f5d)T75L1'.t%i.}

qLIESAfEfR%fB1,%t1%ie

#t%#;'L

tDesuuov1'brjo (

_=fSj%

Bq

fcL16SrRNAi&1f+f3*

ttjT>f%Rea1-time PCR;'ifif] LIT.

AiFqa#jS(=as(+%DesuMoTdbrlo

0)571jEr=%&(=?LITiE*j%Lj=o

[#&t*;.L]

#&.

+giBb{AJ**f5%(+1=*ti52G

(22-51&)

(4*;ll).

#*$513B

(19-45&)3SJ=t>.BEf4S^f4nLjJi

ft?T5t#%5%#LJ=,Sh%88G

(22-81&)

(4*;12)

0)%f+1913B

bt6t#Fxu=BiRr7jrf4*#tu=o

3=1=.

(3)

i;'iOf&;I

lt20)BiAr7 jl=? LIT. Ej5gO) #%Bkie#i%hfj]] L1.

_{qLlbSAi>f&ffB1,%tu=hRJt5aBqie#i%}

a5(21B )fli?T=. i%#%TiO)ie5i%ibbt6Desu1(ovjbn'o

0)L7}ACi%#f5E3f.

L7]iA##

(=?LITF] 2.

tE#

#RS5aTi5t.S&%9SBSLT=.

@4A;12

(=3sLIT.

i%a5P%1iO)ie#i%ibO)-%B(B*$29

i

t9t5k5ai?,Fa%2

1G 0)

i+50*)

(=?Lit.

i%#,.'@bt6hil

tisL1=DNA%if] L1. _Real-time _PCR;'Lfjq L_{tTDesuuov1'bn'o} _f=4Sj%Bq*j:

16SrRNAiaJf+0)*H

fH3>T=o

@4*;43Tli.

iW=(=#&(=hnif=#*$0)BiRr7

jkbt6FLELffh]]

tisu=DNA

fif]

L1.

_B]#(=*RS

_(=fSSEBqtJ

_{16SrRNAiEliij10)4*dj} fH3>1=.

[6B!t%*]

Ef]

;S%[i. B2Rr77o)ie#i%#bt6.

DesuuoTq'brlo

_{0)L7]LA&t%j5(=f&a}

L. DesuuoTq'brlo i)(.E)

_{A2. 5h'1E%}

a)3ftj:6f. B2(=LTEf#-C.f%=t%imd)TBB

6btf=L1=.

ie#t%ibbt6L7JiAEB] a:Lf=##fi

DesuuoTq'bn'o

)'DteSt1'Da1)'stF]2S^T=o D. )'DteSt1'na11'S(i.>Ej71J

0)5FiiEeb16L7}ABS^.

*3B

S^T=LO)T.. =hiTh. t=

Lbt60y7]i#b{6*;PDShTL

ltd:LIZ@LL15#;RTC,A?T=. ie5i%#;'i7f.0)

Desuuov1'bn'o

_0)BiRr7

7b1 6 0)4* tB*[i 3.1%t1&*T-&T3T=b(.

i%#7BS(=Pilitu2Lt=ie#i%#,.Bibt6EFl;#%b{&1fAf=*djLf=

50

4&660)

22%(= Desuuov1'brlo fS

_f=fSj%Bqtj:

16SrRNA

_{ialii+ff*tt T.fT=(4&;1 2)=tbt6.}

DesuuoTq'brjo

f*#PR(=(iie#i%#;'L(=iL)1$6^T=KAcEL)

SL1*T.B2Pqa#jS

EP

[=rfiELTL1%tO)t#;.Ar1S^1=.

3:T=.

BiAr77iL) DNA

_{%FLELffh5tHLT9SBGLT=}

Real-time PCR

(4*;4 3)Tli.

40

_4#0)

_{10%r=JSf=4Sj%Bqtj:}

16SrRNA

iSlf+0)TfjEf6*;PB1%=tb{-C51=.

*W*^(=J= i)

1$6^+=#5Et

DesuuoTq'brlo

_{bSL*BB. iEli}

j1S%P3&FjE85f61fiqf3ef$1%qLIE7K#flt-aljE4&tLTrEi1%=t.

%LT*@#0)

FqS#b{.

B%

_Fqa

50)%N=EE&1%S&LlfEq5fi*f%1%=t%%i%b%bt.

B2Pq

_(=rf#1%

DesuuoT7'br1'O fi. RB25t f4.

5A'1b#5tf4Sat#tB]#.

BEf4*S^f4Sii#(=3sLITt.

Pq

B]12@{jEt5tiii9t4TE#gf,J=BgjiLTL18q

gEi2b{& i).

%i&g5HRi7.Bu

0);#*fcL-4&;4iI{telft%i6^T=o

.i1[Ti7-i-'iFuE#.!,:nt0)!iH-.a

t= I,fL-nSJ.;:I_till)ffii<(T!Ii-['ig-,+rji (4=i i- 73

;I:urL+i;i-,Yi:

7Jt;

-I-(:-(E

L i i1,+IllVifiH-,=i)

rR

t!Lku)3:P]tI;T7)I;t1.I-.LilJttU-)l1-)iit;i,-i-i:}i-i;Q

LL.L 7-IL),][Z;a.r9:[L-i!q7jt-.).'EV)P.F(rkTi fL"-a.I.i V) I.Li!giit-]l'i1)"I) fi)rkf'rt-jJ

,lt3_{+ I)ii_rrf.a)}_bJ(i_{kiTit. i-'IF(/{l'-}_{'x=(fL id11!}J) _-L'f7f.rf_ )))I;/J}F!friLtiL8c _)tqi;.;I _{J)Tit:u)I..i!Si4t-.1.tf:::=i-J-}L I 1 i-:.:-i''6:I J=∼-Hli8nTR::=LJJir.lfEi-i1. #1:tl1/=tu)-(Fft]L't:)I/;S <.

F]/?:1F;),F(L^-h'-&f-^n{fT+,<JI _t=tljltt>71i)I;_Lb73 _a:ui'f.:i,+I

,Jy-?I y 7,(I L I

[kJL-;='qFtli:7)I;/(il)it

/TJ.: L iJ

1L tL1)'Jt;_lB

LF,'F. 4F;Wt&f[VfFki-75

;I:uTi1;)-u):A:

L L

,St,T:1J-I:_7Jt;it-ri'i--LTu1,-J-i 5 :=tLli,).

J5L-(=EJF;),F(IBA

_L L Ti,;et;;7ttLitilT= i Ju

itT.;iTJt-.abH I_-G<(I-:

,r;T._:q) ,j}j^ti1Wft,;.-I(:a) jT:.i-frrI-_i:% d)f=fII'i1',qi'T';ii7-II-i=a) fH EiJ1-:lT]:I -i,.JIF3),F(.r'I:jJt;4F;-fRt2.

,i_r,

N-ZT'tTr-8"pathogen synergy" i:Vl 5

4BEB:b';.ai

P 5 ilT % f=.

S I , 6 -PTOteObacterla W i=h%+8 DesuuoTr7'brjo

Ej:##i5#ni&/*1t-dJB?iE4hpC>b

8

#rB%uA(#1'

-A

LLJht,iEPl-1iJf".;1-iJ.i-ulfj L -I11E'I^L.I:+7D yTt_[t:.tJI!T.I,i-cRcjE;.jt;'t.M['f';ia) --frrL:Tt,

i:T77i-:a-,Y3

Tt

T'ifin;it!fi[iU) 177(-a'''!fl::'-Mf7t-iFLIBii_1 'fkL7ErJ_,lilli).J3 I i.,

,-5-['i;Hr1;-Ct_L<r)7J.; t[,,--+ffif(I i 7J 7 7- P --fTli-1= a).'}7Y:I.rtfEiri17ilriT=t.(]Tx:I

/JJWEret

5 I ilf=.i L I i,rr-717ii'fJt;BjW;;jLlf=d). i-L]tLES it-i, _{i J3} _{Tn7tET_!1i/J)1;}

I.:.ii,/u)(ilil(f'tT&7v)---1 Tt;ba I. ;FrF.

.;)-I.14ir-lFt'l'Jj= ･Ii-.-:I

(i8 -a;ifJia) rJ],Jt')'Jt;7TIHiii.fLt-? i-II i /)

, _Lt3. ll.1'7

i-;:i1-T,:-1h'6,+Ii-1.;)"'lLtf1(V;i-:iX6r)i'J)-I-ui.)if_[9-lJJJfq)_JL.:I:1<lk..ii!l':.a) '})f!!ft irLTr-I-iJt;llt7'..))nL I i. 7i),

,1=-&llFlf-[i}J1=(I(i-7T;tLlf,itfL:A(ii:-,iJl7E_'t+,5f2/)

,. _I a)

1i

I)_{!i1,.i!M5.)Ltd;I.i-J-}_{L /-HJ} _3't3

.[1jj.int)(=3$1-JILT>._,it,1J I._L )'Jt;Pyi ,

L{,)7)ftTt:u)r)L[i'f'{i:tluii= ff:;F2 rrru-I.r[Ji(tD,3i1EirTrT)-.flJ]iftlp1:F.i,J-I

fT-I-

,<Ji-IN)Ti-]'&-L';.5 /illL

1

/')1;.

^1 r';_:)-Lr7'.:fJ1;rAJ-tj-Tl-_,5I.i!qLLM;Ea).f>rU-r..ifT*.1'.I:)i(I

JLy.-%itLIIafniJ-i-;;I:u

['f.:iJt-;:':[']'J.FTfLif-ri/)I;

5tf/I)i tir-:,-i I_LIiIL5l:I)i-:I

(4)

(9'lJi tji+._-1=-L!f'l'irlf'-:a)lJ'iQJ)13:tl]i+;t]-,t'Ii':I;L' :+ ,3I.I;i';(jI.rib;IrigL -IT-i.F-h--Hi;I/;_{j( I.-J;;}L -Ii. _}3

=. ;i-=[J-)F7EvTt:i.lll',.L'f'l-1-.:I.-- rE:I;.i:-LLJ'JJL-)I-!-I:-.gbJ:-;'':n'J-T=tilf>L(lHiI, iJt;A/1tj:.i->i.,i--i:E-,!1_tJ1.1'..,J31 t U;7.1.T'..)J--:I.+L.Jf-,li-J1..:;-/9L -i=..rL1./-K-Ln.I.T,fI-23 1 I(.,iJ

x-LIB

i L/C , l1'BPq8=h' C18 Desu1(oTq'brjo

0)GTj1*:tE=-jb

lrC4*il

L,f=.

jT.I.:nl..i.L _{TE7t,iE(k3:,I)Jr-3)'q'i-far:I:n!,}+8 l[.l:,]L'r':fl-:i/I6-u:-:I

,_i,31&-i--Jr=t.-!KIJ_I!191 f. [lJG r. (3.1(;:,);/J

,_{i_I).}

1(I-;:]-4!-'f':.iJt;_-1I::I:/J:)1.'m'iLi.-iit -/I.._.5}RfEL f= _L1llia) iE11I!_ifkJ:-i.1['l'J4'!iirxf-i.3u_qI-,))Jf.!E,ir,fJ-TT-TJILlft.1=(1J1!)!t!::fj.:lJh//I)i rJ':I:fla)

tIlq(IhL -/=t-FIT:i.1f:iT.. --Ttt7JL 7 _:.)-I=ttU ll:>.;1't.[h:.)![JPl]].fI1!.}J

,J1--/

-t}l.'l),.fli.

(rlrjr_9-i(cllf".;3Eitjtr:i,1j.r!J-:1 )J[l_I;-f-I.-i!gJ11J1:_-Lit;tL,)J3

f=L. (JJL-I-/ir2)I;illr')()tLIFf]''i:a);I,:i4t._-1 1;1:EiI,-hi/:-L':It= 7,_a)I.i!Hi45i+rt)7)Ht_) _;7Y,rfE-:_i<_i1.i/i_'T=

-I t-{7f[rL:2 subt},pcs

(Desu1(oTr)'bL.1'OP)'geL; D. desurhn'caDS MB subtype, D. desuuun'caDS Essex6 subtype, 'DesuuoTq'bn'o (a)i.LFeldeDSIs', Desu1(ov1'b).)'o _vulgan's) n V_{'i!al i i}

-5%ii8

65i@re^

A 8 Desuuovlby1'0 1'nteStlDa11'S i B] 'iii'i.;Tt]=o D.)'D(Pst)'D(L1)'b.Ei.i t= 7' U _a).ii'j

_[L:Jli:/J.

r3_/J:JYlfE-_S

,-i,i1-ILL.a) i.i-Fi;I.--)5t;LJ(,)7D a)'7JJ,=t.t= T,))J,ttjU) 71-i-Li-_:J-rrrL-.

nJ{DtmEEi*WgEb1;A.inre.ba. n*iiEb5ko)4

_{r#Ej:Vliinb4.}

B iAj]]

5YtJ8.Ib*#rehb8

ttoJ<5i.J U >, tto

J<7.J U >. 9 '}/<9 9 L8=IrEf7;L2WIJr&k'j%L,TUlf=o

I u) (ui,

,7t=-LT-)FgEretEi!ti!WxFa)ff_tLlf;,:-IStIIf,-ir,

Tt[I1-;)

_1j!ITl

iL-,JiJIJAi-;)-L f=..;ET',iE(kS.I);)r-3))UJt;i;u,Ji).;i.L fit)')1J3 _-r=l-.:';-fg

tft(.LIL-_i.lriTfi7,7 77) ,

LB-AJl&-JII1[LiiL 7= DNA lj!(1=,+fJ*j._{f!(I.Deslt1(ot7.b).)'o(=4-!f.._i.yltJfLt-i- /)_I

> 7jr y - I)ll

y I,E= L,f= Real time PCR i:11J3f= i I a,

66%-clj:

50 1FfJqjll q(]

(220/.),i&%-Clj:

40

1Fr]*

4 qfJ(10%) Tt DeslZ1(otr).b).jo/J)I;I.'t),-.r!I:_i./illJ3 7=c I ittt_,V) /}J1-I-I:i:-_n!'=;.'=n'J=T:ii:-i= A: 8

1j:,i-;[#.A:ill.75

,tt,

-l']J[U-r=1-,3i. ;b 75t;rTITa) kLItiq)^= rf;{1-

fr1'i(.T'TTI.1..?.-

fi /jkBJa) _F)iE_{(ki-:)Jr-3r)i}_,li-_i-BT,LTlti.+ ,-Jll.l_1'-L't:J-I;j'-i''(;_tii:.-Tlf3--it3it f=fL-Ef

_ii)F.ii

I_I

LL?)I;net/lti'LTt_,-ll

f=. 3:f=, I _=T>-ak'LjE'i_Lf= Real time PCR Ej:,

_{RfLigve>,t%iitE=tEJ<SdSL2t3:4EHibre>b8}

I i bS,j%5 YLf=.

i). L__{/1=.i-[1i73cv=.}fj:f[,.--fSti=tiT-Jli: Ull/I:JtI-!Iiqbl;-;.'--r'1tI)I-tli;-:I i r). DeblZJR)I,)'b}.)'o'=ylJ':7f.[),I(If!1S:(l-=f-7i)_{klJI!v)-(;.:-fF_} i }LTa) _1*

[Lt!I.Slti:I-3 i ,rL, a)-lt1)i-ii: i)Ji))Tf,Fi!7FL f=L,

f^ _f=.

lJ'-7!iJ"I_,.'77iF!fE_L5:hf=i'f':J-TITI-.7Jt;t= 1,)7)1

tBU)!}-JY.Lt,rtEftiff(1r] b';tfA,J3 f= D.)'DteSt1'Da11'S_vChbJ3 f=I i

b#*p5avC>b

8.

As##AS#+8

I i:b';'j%5 YLTV 18 5&V IPqi5*f ifli _{_i..} lli-;;fn'-:7TJ1;.e.:s1;r'T()_{:I li'(_-:+:+}_,3Flit(E/)(3;.:-v)i:u)JB-iL;:1JIi.)u_T1.j'-iif.r'1:_.4:,.1[J1::(Li'lRJrirLh'-i- L I V) _fT=)H_,+I_Fg)

I,S-+

73

Lh. l]'_BPqE=

]f--7--TT_T1-

8 D.)'DteSt1'mL11'S Ej:, I;'Li::_i-tTI1.l',.)J.-:I.fit;1:J!kLu)rJ,lP_qJr_1:_I.i!Mt-.)ql:u)I.FtL)()i+;;i.isit3i=EiJ;-1r.J.;Ji-LL

I),]1+ fC StS.Jif-:1!Ii3ii.LFLIU)a.uJ_{L'{':iflI:_fT.Y,I}fTi7:r7_]+ a-)_([)'1:

TLfh-i L I i?;P;}1lI5 ii,3I-ii /T)ff'xi.ft_1:Jt;]-T. {v),3I i i,-,T,-[Tk5 it_,=i).,

I _n3altj:, _{=a1 6}

_a)iW*p5a

2= DesuuoTq'bn'o 0) t= I,

_3hF6i#

i L/CO) 5 6 ii 8

_{q#Jr*k'j%i-Jr##k%A/e>}

J3 r). _{Jlj:ijlF_U);:Lt.:nt.}_I _I);i-I:iT

i->iU-if:(iL7lTi7i-:IfLlf1:+/-a

LL._-l!uJ/ii'L f=._.

Ill{-_{a''!S.i=t.[.i:.j!}jE.I.:nl-a) _{* a}

I _a).;l[:iT_{i-a) ji tip.[;--r'.'7}Et.}_'1i;:-itfH.5..--[;i73ti: L I (1]A1]Ju[_[TV) 1_i'l"i_{a),i'lTf7-EvTt}

(I,)73 : I J).i-lllili'!)I;I::;'':(1I_I:.i-I:iT-i-Li-L I '7LE

rJqcT5ilf=r)lJii-,-'r(-f+7J I i _{,=YIT-I(E.ii).}L f=:.

1tl[.Af.i>;I.1-3i _tT. _{J'-;::(iE-.;-[:i?}_i-U)[),JJTlf',+i-_tlJ

,i.LL.L -I. I i-L:I r-1yr.:Jiif+7ulJT-1-/E];.T1-fit;7)-E)51f!.fE.L'1!h.)-7EPI-:'fjEiL7ti:u

[li1-DesLZuOT,)'b).)'o_{U)3:u]f_.r':f'1i::r'1FJ.}Laf;,-.I-):6':u]i.Ii.iJ:i::n'J_,i,::Ei-i'iuL.fllu:I);.5 Lt_):I 4[fi2U) _li$13;.1+:J_lil)]r.FLa.)_1t3:.-*r4!_fitill:I 1 i1

vr i"i-if,:f7!]_7LyI(1--? 7-=L _-uffTi7-1-II1;:i.J--]#/J).it). jiiJ(7)<iit[),J7;i_,+ri--!i--3i-IW },]=;J1;'fTTfi:-)it f=J) pet. _{1rli.n.I:1i-.5t.Lljhi=}i/LN.JtiL f=

t*JEL,flo

(5)

Suguru Ichiishi, Kaori Tanaka, Kenichi Nakao,

_Koji

Izumi, Hiroshige Mikamo, Kunitomo Watanabe, First isolation _{ofDesu1{oTq'bn'o} from the human

異化性硫酸還元細菌に関する臨床細菌学的な検討 : 膣内細菌叢におけるDesulfovibrioの存在について

Title