抑制性シナプスにおける逆行性伝達物質の作用およ びその役割
著者 少作 隆子
著者別表示 Shosaku Takako
雑誌名 平成10(1998)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要
巻 1997 1998
ページ 2p.
発行年 1999‑12‑07
URL http://doi.org/10.24517/00066025
Creative Commons : 表示 ‑ 非営利 ‑ 改変禁止 http://creativecommons.org/licenses/by‑nc‑nd/3.0/deed.ja
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1998 Fiscal Year Final Research Report Summary
Roles of retrograde messengers at inhibitory synapses
Research Project
Project/Area Number
09680813
Research Category
Grant-in-Aid for Scientific Research (C)
Allocation Type
Single-year Grants
Section
⼀般
Research Field
神経・脳内⽣理学
Research Institution
Kanazawa University
Principal Investigator
OHNO-SHOSAKU Takako Kanazawa University, Fac.of Med., assistant professor, 医学部, 助教授 (60179025)
Project Period (FY)
1997 – 1998
Keywords
Hippocampus / Inhibitory synaptic transmission / Retrograde messenger / Synaptic plasticity / Calcium ion / Glutamic acid
Research Abstract
1.Recent studies have suggested that retrograde messengers might play an important role in synaptic modulations and plasticity in the central nervous system. In the hippocampus, depolarization of a CAl pyramidal neuron elicits a transient suppression of inhibitory synaptic inputs to the depolarized cell. This phenomenon is referred to as DSI (depolarization-induced suppression of inhibition). The studies with hippocampal slices demonstrated that a retrograde messenger should be involved in DSI.
2.In the present study, we examined mechanisms of DSI and the role of the retrograde messenger in DSI with cultured rat hippocampal neurons.
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Published: 1999-12-07
Research Products
(10 results)All Other All Publications (10 results)
URL: https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-09680813/096808131998kenkyu_seika_hokoku_
The results and conclusions are as follows.
(1)A pair of cultured hippocampal neurons can exhibit DSI similar to that previously reported to occur in hippocampal slices.
(2)Extracellular Ca^<2+> ions are prerequisite for the induction of DSI.
(3)The inhibition of voltage-dependent Ca^<2+> channels suppresses DSI.
(4)The release of the inhibitory transmitter from presynaptic terminals is suppressed during DSI.
(5)The protein kinase C activator (phorbol ester) suppresses DSI.
(6)The inhibition of CaM kinase II suppresses DSI.
(7)DSI can propagate from the synapses on a depolarized neuron to those on a non-depolarized neuron.
3.These results support the following hypotheses accounting for DSI.Depolarization of a postsynaptic neuron elicits an increase in postsynaptic intracellular Ca^<2+> concentration by activating Ca^<2+> channels, which in turn triggers the release of retrograde messenger via CaM kinase Il- dependent processes. The activation of presynaptic receptors by the retrograde messenger induces suppression of inhibitory transmission via phobol ester-sensitive processes.
[Publications] Takako Ohno-Shosaku: "Modulation of inhibitory transmission by retrograde signaling in cultured rat hippocampal neurons"
Neuroscience Research. Suppl.21. S60 (1997)
[Publications] Takako Ohno-Shosaku: "Properties of depolarization-induced suppression of inhibitory transmission in cultured rat hippocampal
neurons" Pfluegers Archiv. 435. 273-279 (1998)
[Publications] Takako Ohno-Shosaku: "Possible involvement of retrograde signaling in modulation of inhibitory transmission in cultured rat
hippocampal neurons" The Japanese Journal of Physiology. 48 Suppl.S20 (1998)
[Publications] Takako Ohno-Shosaku: "Calcium channels involved in depolarization-induced suppression of inhibition in cultured rat hippocampal
neurons" Neuroscience Research. Suppl.22. S97 (1998)
[Publications] Takako Ohno-Shosaku: "Slow synaptic responses and modulation./Propagation of depolarization-induced suppression of inhibition in
cultured rat hippocampal neurons" Springer-Verlag,Tokyo, (in press)
[Publications] Takako Ohno-Shosaku: "Modulation of inhibitory transmission by retrograde signaling in cultured rat hippocampal neurons."
Neuroscience Research, Supplement. 21. S60 (1997)
[Publications] Takako Ohno-Shosaku: "Properties of depolarization-induced suppression of inhibitory transmission in cultured rat hippocampal
neurons." Pfluegers Archiv. 435. 273-279 (1998)
[Publications] Takako Ohno-Shosaku: "Possible involvement of retrograde signaling in modulation of inhibitory transmission in cultured rat
hippocampal neurons." The Japanese Journal of Physiology. 48 Supplement. S20 (1998)
[Publications] Takako Ohno-Shosaku: "Calcium channels involved in depolarization-induced suppression of inhibition in cultured rat hippocampal
neurons." Neuroscience Research, Supplement. 22. S97 (1998)
[Publications] Takako Ohno-Shosaku: Slow synaptic responses and modulation./Propagation of depolarization-induced suppression of inhibition in
cultured rat hippocampal neurons.Springer-Verlag, Tokyo, (in press),
