口腔扁平上皮癌の転移形成に及ぼすマトリックスメ タロプロテナーゼの影響
著者 熊谷 茂宏
著者別表示 Kumagai Shigehiro
雑誌名 平成7(1995)年度 科学研究費補助金 一般研究(C) 研究成果報告書概要
巻 1994 1995
ページ 2p.
発行年 1997‑03‑03
URL http://doi.org/10.24517/00066428
Creative Commons : 表示 ‑ 非営利 ‑ 改変禁止 http://creativecommons.org/licenses/by‑nc‑nd/3.0/deed.ja
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1995 Fiscal Year Final Research Report Summary
Roles of Matrix Metalloproteinases in Metastasis of Oral Squamous Cell Carcinoma
Research Project
Project/Area Number
06671997
Research Category
Grant-in-Aid for General Scientific Research (C)
Allocation Type
Single-year Grants
Research Field
Surgical dentistry
Research Institution
Kanazawa University
Principal Investigator
KUMAGAI Shigehiro Kanazawa Univ. School of Med. Asst. Prof., 医学部・附属病院, 講師 (00215013)
Co-Investigator(Kenkyū-buntansha)
YAMAMOTO Etsuhide Kanazawa Univ. School of Med. Professor, 医学部, 教授 (00092445)
Project Period (FY)
1994 – 1995
Keywords
Oral squamous cell carcinoma / Lymph node metastasis / Matrix metalloproteinase / Immunohistochemistry / Gelatin zymogram / In vivo model / Clincal materials
Research Abstract
In order to study the roles of matrix metalloproteinases (MMPs) in lymph node metastasis of oral squamous cell carcinoma (SCC), the expression of MMP-1 (tissue collagenase), MMP-2 (72kDa gelatinase/type IV collagenase), MMP-3 (stromelysin-1), MMP-9 (92kDa gelatinase/type IV collagenase)
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Published: 1997-03-03 URL: https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-06671997/066719971995kenkyu_seika_hokoku_
and TIMP-1 (tissue inhibitor of metalloproteinase-1) in oral SCC tissues obtained from biopsy specimens of 46 cases and from in vivo metastasis model was examined immunohistochemically or biochemistrically. Among these MMPs, MMP-1 and MMP-9 were immunolocalized in the carcinoma cells in 70.0% and 45.7% of oral SCC,respectively. On the other hand, MMP-2 and MMP-3 were positively stained only in 21.1% and 10.5%, respectively. TIMP-1 was immunolocalized in 22.2% of the cases, but the ratio of immunoreactive cells to the total carcinoma cells was only 0.6(]SY.+-.])0.2%. The ratio of positive cells for MMP-1 or MMP-9 in carcinomas with lymph node metastasis was significantly higher than that in carcinomas without metastasis. In in vivo metastasis model, gelatinolytic activity was higher in the culture media of OSC-19 cells (high metastatic phenotype) than that of OSC-20 cells (low metastatic phenotype). Gelatin-substrate gel electrophoresis also showed that the gelatin-degrading activity with molecular weight of 92.000 was higher in the culture media of OSC-19 cells than that of OSC-20 cells. These results suggest that MMP-1 and MMP-9 play pivotal roles in the degradation of extracellular matrix macromolecules during oral SCC metastasis.
