Phytoremediation of arsenic by aquatic floating macrophytes

Phytoremediation of arsenic by aquatic floating macrophytes

著者 Rahman Mohammad Azizur

著者別名 ラハマン, モハマド アジズ

journal or

publication title

博士学位論文要旨 論文内容の要旨および論文審査 結果の要旨／金沢大学大学院自然科学研究科

volume 平成21年6月

page range 72‑74

year 2009‑06‑01

URL http://hdl.handle.net/2297/26912

氏名 学位の種類 学位記番号 学位授与の日付 学位授与の要件 学位授与の題目

ＲＡＨＭＡＮＭＯＨＡＭＭＡＤＡＺＩＺＵＲ 博士（学術）

博甲第１０５７号 平成２０年９月２６日

課程博士(学位規則第４条第１項）

Phytoremediationofarsenicbyaquaticnoatingmacrophytes．（１ 遊性水生植物を利用したヒ素のファイトレメデイエーション）

長谷川浩（理工研究域・准教授）

上田一正（理工研究域・教授)，国本浩喜（理工研究域・教授)，

平山直紀（理工研究域・准教授)，牧輝弥（理工研究域・准教授）

論文審査委員（主査）

論文審査委員（副査）

英文要約

Thephytoremediationofarsenicbyaquaticmacrophytes⑰加北、polｳﾉ抽吃αＬａｎｄ 乱れ加財〃qZc7"ｓＬ・wasinvestigated,P1antsweregrownhydroponicallyonMSculturesolutiolL

Arsenicuptakewashigherfrolnphosphate-deficientsolution，whenthesourcewasarsenata Arsenicuptakcwasnotaffectedbyphosphate,ＷｈｅｎｔｈｅｓｏｕｒｃｅｗａｓＤＭＡＡ・

Arsenicandphosphateuptakewasnegativelycorrelated(p＜0.001),andarsenicand

ironuptakewaspositivelycorrelated(p＜0.05),whentheinitialsour℃ewasarsenate・Arsenic

surfacesTheconcentrationsofAs(V)andAs(111)onplantssulftlceswereabout43％and58％

higherafterFe-plaqueinductionthanwithoutFe-plaqueinduction，respectivelybFe-plaque inductioｎｄｉｄｎｏｔａｆｆｂｃｔＭＭＡＡａｎdDMAAUptakeandadsorptionTheaqUaticmacrophytes mightusephysiologicalandadsolptive叩takemechanismsfbrarsenicuptakeandadiffbrent mechanismfbrMMAAandDMAAuptake

TheEDTAincrcasedabout4-6％ofarsenicuptake，ｗｈｅｎthesourcewasarsenateor arsenite,whiletheUptakewasnotaffもctedbyEDTAwhenthesou1℃ewasBmWLAand

本文

Phyto焔mediation，aplantbasedgreentechnologybecomespromisingtoremediate envirDnmentalpo1Iutionduetosomeunavoidablelimitationsoftraditionaltechnologieslnthe p1℃sentstudy>weinvestigatedtheuptakeofarsenate(As(V))anddimethylansinate(DMAA）

bywidelygrownaquaticmacrophytes⑰〃zｵｾﾉｍ７ｏ<Ｗ６ｈｉｚｚｚＬａｎｄ肋ん〃α〃ａ間zz7びＬ･to

P1antswelegrownhydroponicallyonstandardMurashigeandSkoog(ＭＳ）culturesolutions fbr6days・CompaMtothecontroltreatment，theaquaticInacrophytesaccumulated signifIcantlyhigheramountoFarsenicfiomphosphate-deficientsolution,whenthesourcewas arsenate､Howev飢arsenicuptakebythemacrophiyteswasnotaffbctedbyphosphate,when

thesou1℃ｅｗａｓＤＭＡＡＴhe⑰加乙Ⅵg/Z7pojiWlh伽Laccumulated51-fbldarsenicfromarsenate solutioncomparedtothat丘omDMAAsolutionwithinitia】concentrationsof40MandO､O2

Mofarsenicandphosphate,respectively

-72-

ArsenicandphosphateuptakeintotlleaquaticmacrOphyteswasnegativelycorlelated (P＜0.001),whenmitialsourcewasarsenatewhilethefewasnocorrelationbetweenarsenic andphoSphateUptake(p＞0.05),wheninitialsourcewasDMAA・Theresultsindicatesimilar sorptionmechanismsofAsO43~ａｎｄＰＯ４３~fbrUptakecarrie工Similarly，significantcorrelation (ｐ＜0.05)wasobservedbetweenarsenicandironuptakeintissuesofaquaticmacrOphytes，

wheninitialsourcewasarsenatewhilethecolrelationwasnotsignificant(ｐ＞0.05),when

mitialsourcewasDMAA・About５６％ofthetotalarsenicwasdistributedmtissucsofthe

plantscomparedto４４％mcitrate-bicarbonate-EDTA（CBE)‐extmct（Fe-plaque）ofplant surjklces，whentheplantswe1℃grownonarsenatesolution・ArsenicuptakeintotheaqUatic plantsftomDMAAsolutionwasneitheraffbctedbyphosphatcconcentrationsnorcorrelated withironuptake(p＞0.05).ThercsultssuggestthatadsorptionofarsenateonFe-plaquesof theplantsurfacescontributestothearsenicuptakegreatlybThus，arsenateuptakcinaqUatic plantsmightOccurthroughthephosphateuptakepathwayandbyphysico-Chemicaladsorption onFeplaqUesofplantsurfhcesaswelLTheplanｔｓｕｓｅｄｉｆｈｅｎｔｍｅｃｈａｎｉｓｍｓｆｂｒＤＭＡＡ

ｕptake・

Ｔｈｅ⑰伽cｺﾞｾﾉﾋzpoMi伽uptakeshigheranlountofarsenic，whenthesourcewas inorgamcarsenlcspecies（arsenateorarsenite）whiletheuptakewasnegligible,whenthe sourcewasmethylarsenicspecies（ＭＭＡＡｏｒＤＭＡＡ）TheconcentrationsofAs(V）ａｎｄ Ａｓ(Ⅲ)inCBE-extractsofthemacrophytegrowMfter]Fe-plaqUeinductionwereabout43％

and５８％higherthanthoseplantsgrDwnwithoutFe-plaqUeinduction,respectivelylheresults suggestthatasUbstantialamountofarsenateandarsenitewasaccumulaｔｅｄｂｙ⑰加娩m pol1W/i〃Lthroughthephysico-chemicaladsorptiononFe-plaquesofplantsuIfaces､TheFe‐

plaqUeinductiononplantsurfhcesｄｉｄｎｏｔａｆｆｅｃｔＭＭＡＡａｎｄＤＭＡＡｃｏｎｔｅｎｔsintissuesand

CBE-extractsofthemacrophyte

Theinf1uenceofethylenediaminetetlaaceticacid(EDnL)onthearsenicspeciesuptake bySP加庇mpol1WlA〃Lwasinvestigated､TheEDT3Aincreasedarsenicuptake,whenthe sourcewasarsenateorarsenitewhilctheUptakewasnotaffbctｅｄｂｙＥＤＴＺｋ,ｗｈｅｎｔｈｅｓｏｕｒｃｅ

ｗａｓＭＭＡＡａｎｄＤＭＡＡ・Itwasestimabedthatabout４６％ofarsenateorarsenitewas

desorbe｡/mobilizedfiomFe‐ｐｌａｑＵｅｓｏｆｐlantsu曲cesbyEDTAwhilethe desorption/mobilizationoforganoarsenicspecies(ＭＭＡＡａｎｄＤＭＡＡ)didnotoccurbythe additionofE]DIAItmightbebecausetheco-precipitationofironoccumsonlywithinolganic arsemc，especiallythearsenate・PhosphateuptakewasnotaffbctedbyEDpMhoughits

concentrationinCBBextractwasmuchhi2dlerthanthatofplanttissues・Ironuptakeinto

extractmdicatethatthemcreaseofinorganicarsenicuptakeintotheplantbyEDmMSrelated toironuptake．

－７３－

学位論文審査結果の要旨

_…壬△笈.腫一ＺＺ式.…乏心扇迂氏よH1提出壹妊迄霊位論Ｘに関しＸ１－畳審査;鶴、掴肌二審鴬した後…

霊威雲２０.窪_Ｚ員_lpL且腿審j蘆員｛こよゐ孟傭審査会塗襄'施丈蓮と.と測三､_要Ejii-jZO_集&月-回_剛二且襲溌表会 と論文審査委員会を開催_Ｌ,__以工の_よ_豆に判定Ｌ左１－－－－…＿…－－－－－－－－－－…＿………－

－迄素撒染溌瀧亥ﾘ撫以父芝烹ｆｚ詔｣z)Li1i匙M1凹篭rLは灸一三八上面から衛劫溌溌l旧購丞限_鐘れゑ患友・…嵐

然辻鉱’雲上蓬泡{閉上上難境修復技術と上重.Ｚｚ式上ｋＺ薑烹ごf壬烹Ｚ且ZZl軋睡貝塗集い:、1重且_凌班究 重住Ｊ苞If堂jir嵯基づ.§_捷化j去囚確立を鳳的と上玉｡‐現地よ_、採取.L・た漫遊i生水生植物、虫血且巨素査 選１Ｋ剛皇高搬繊式至極産搬索式動と_と典Ｉ具､.､證囚nＭ込み挙動囚議jliml塗検討Ｌ上｡_罵連!Z、究嵯鐘k）

:豆_､一生佳内企n2L諾上ﾋｰﾋｰ素.の２ＭK劇茎鯛幾陰嘉よ_n造著_し三_山鹿k'二と_堂_確認_し９－眞議}主Z(_壬と化臺 ltL灸と_phD【t卿錘馳jlj奴_鱸物訂思蝋__塑鰄i生式畳_二と．を阻良なにＬ迄.－重迄画__ユー価怨機迄素囚絃鋼腿企 1ZZfM.鰹丑(三闇ＬｎＪﾐｭｰ.化裳的燐質Ziﾐ類(』身上:ｉご_葱_!/LZj駿上j吉抗式ゑこ_と迄示した１－夏(ﾆｭｰ槙{M1lj細胞Ｉ皇猫 Ｉ土動匡素の化裳溌態別分振迭_を確立まる_と．と.測皇，一同獲迩蘆思.LL五根近傍1三鐙は鼠違憲囚化鍵種jitJiii を観澱｣し貧Ｊ鰯罵璽(二jHi成.さ#:とi二曇k粒王層蕊細胞、冒冒Ｑヒ素ZL取、鰹轟塗鵬薑冒ｳ冒固亘二．と産見虹麓しぬ

＿払上｡－本駈寵瞳､_淫遊i生Zk熊植物Ｑ_ﾋｰ素j1Mj1L丑議動JiPL農素化裳jmiQ雛幾．遂力蕊.閉止した－１Ｍ９１重迄

ｊＬ_蝿蝋競腫撞は重濤匡_素汚染｣(､携化』Ｅ煮思な知見を提鍵式畳駈究と_L忽玉憾土_(裳伽_Ｑ學菱位.に値まる ＭＰｌと判断した.－－－－－－－－－－－－．－－＿_…－－－－－－－－－－－－－－－－－…－－…

－７４－