㧙 㧙
52.೨┙⣼≸ߦኻߔࠆ photodynamic therapy
ේ⑲㓶1)ޔผ1)ޔၫශᄥ㇢1)ޔୖᨋ ⌬2)ޔ㒠ᐈ⌬ᄦ2)
1)㜞⍮ᄢቇකቇㇱᴲዩེ⑼ቇᢎቶޔ2)㜞⍮ᄢቇකቇㇱ∛ℂቇᢎቶ
(⋡⊛)
5-ࠕࡒࡁࡉࡦ㉄Ⴎ㉄Ⴎ(ALA)ࠍ↪ߚ photodynamic diagnosis(PDD)ߪ⣖ᄖ⑼㗔ၞߦ߅ߡᐢߊ᥉
ߒߡ߅ࠅޔᴲዩེ⑼㗔ၞߦ߅ߡߪ⣾⢲≸ߢߘߩ↪ᕈ߇ႎ๔ߐࠇߡࠆޕ೨┙⣼≸ߦ߅ߌࠆ ALA ࠍ↪ߚphotodynamic diagnosis(PDD)߅ࠃ߮photodynamic therapy(PDT)ߦߟߡߪߘߩ↪ᕈ߇ႎ๔ߐ ࠇߡߥޕ࿁ᚒޘߪޔ೨┙⣼≸⚦⢩ᩣߢࠆ PC-3 ⚦⢩ߦኻߒߡޔALA ࠍ↪ߚ PDD ߅ࠃ߮ PDT ࠍⴕޔߘߩᴦ≮ലᨐ߅ࠃ߮⚦⢩ᱫ⸃ᨆࠍⴕ߁ߎߣߦࠃࠅ⥃ᐥᔕ↪ࠍ⋡ᜰߒߚޕ
(ኻ⽎ߣᣇᴺ)
PC-3⚦⢩ߦ 0.1-1.0M ߩ ALA ࠍᷝടߒޔ0.5-3 ᤨ㑆ၭ㙃ᓟޔPpIX ߩ↢วᚑߦ㑐ߒߡⰯశ㗼ᓸ㏜߅ࠃ߮
Flowcytemetry(FACScan)ࠍ↪ߡ ALA Ớᐲ߿ၭ㙃ᤨ㑆ߣ⚦⢩ౝ PpIX Ớᐲߩ㑐ㅪᕈߦߟߡߩᬌ⸛ࠍ
ⴕߞߚޕ
PC-3 ⚦⢩ߦ 0.5nM ALA ࠍᷝടߒޔ3 ᤨ㑆ၭ㙃ᓟߦ PDT ࠍ 10 ಽⴕߞߚޕᾖⵝ⟎ߪ TheraBeam
VR630(150W Na-Li lamp, ᵄ㐳 550-740nm (2 ፄᕈࡇࠢ 630nmޔ670nm))ࠍ↪ߚޕPDT ᓟߦ Anexin-V
߅ࠃ߮ PI ᨴ⦡ᓟߦ FACScan ࠍ↪ߡ⚦⢩ᱫ populationޔcaspase-3 ᵴᕈߩ᷹ቯޔࡒ࠻ࠦࡦ࠼ࠕ⤑㔚 ߩ᷹ቯࠍታᣉߒޔࡒ࠻ࠦࡦ࠼ࠕ⚻↱ߩ⚦⢩ᱫߢࠆ߆ߤ߁߆ޔcaspase ଐሽᕈ⚦⢩ᱫߢࠆ߆ߤ߁߆ޔ ⚦⢩ᱫࡔࠞ࠾࠭ࡓߩᬌ⸛ࠍⴕߞߚޕߐࠄߦޔNOC-18 ߿ deferoxamine ߥߤߩ ferrocheletase 㒖ኂࠍ↪ ߡޔPC-3 ⚦⢩ߦ߅ߌࠆ⚦⢩ౝ PpIX ߩ⫾Ⓧଦㅴലᨐ߅ࠃ߮ PDT ߢߩ⚦⢩ᱫߩଦㅴലᨐߩήߦߟ ߡᬌ⸛ࠍⴕߞߚޕ⛯ߡᜂ≸ࡕ࠺࡞ࠍ↪ߡ PDD/PDT ࠍⴕޔᴦ≮ᓟ⚵❱(⣲≌)ߦ߅ߌࠆਇนㅒᄌᕈ ㇱߩഀวࠍ 2 ᰴరߢ⹏ଔߔࠆߣߣ߽ߦޔanti-single stranded DNA(ssDNA)᛫ࠍ↪ߚ∉ᨴ⦡ᴺߦ ࠃࠆ⚵❱ቇ⊛⹏ଔࠍⴕޔߘߩ᛫⣲≌ലᨐࠍ⸃ᨆߔࠆޕ
(⚿ᨐ)
PDTߦࠃࠅ⣲≌⚦⢩ߪࠕࡐ࠻ࠪࠬࡀࠢࡠࠪࠬ߇⺃ዉߐࠇޔPC-3 ⚦⢩ߦኻߔࠆ PDT ߪ↪ߢ
ࠆߣ⠨߃ࠄࠇࠆޕߐࠄߦ PpIX ↢วᚑࠍᓮߔࠆߎߣߦࠃࠅ PDT ߩᴦ≮♖ᐲߪߐࠄߦะߒޔᓟߪ ⥃ᐥᔕ↪ߦߟߥ߇ࠆน⢻ᕈ߇ࠆߣᕁࠊࠇߚޕ
