Fig. 16 Detail of Type II Japanese native linage. The dotted line is an enlarged tree.
Number in parentheses are the number of individuals.
Ishikawa, Nozaki (8) Ishikawa, Anamizu (20)
Ishikawa, Nagasaki (6)
0.02
Type II
Table 1 Comparison of the characteristics of each dojo loach specimen collected from the Naka-ikemi Wetland, Fukui Prefecture, Japan.
Specimen no.
No. in
Fig. 3 Date of collection Sex mtDNA lineages
Total number of vertebrae
Nuclear DNA lineages
Total number of AFLP bands
1 A 23 May 2013 Male Type II 47 Type II ―
2 a 23 May 2013 Female Type II 47 Type II ―
3 a 23 May 2013 Female Type II 47 Type II ―
4 a 23 May 2013 Male Type II 48 Type II ―
5 b 24 June 2013 Female Type II 46 Type II ―
6 b 24 June 2013 Male Type I 48 Type I ―
7 b 24 June 2013 Female Type I 49 Type I ―
8 b 24 June 2013 Male Type I 50 Type I ―
9 b 24 June 2013 Female Type I 48 Type I ―
10 b 24 June 2013 Male Type I 49 Type I ―
11 b 24 June 2013 Female Type II 47 Type II 87
12 b 24 June 2013 Male Type I 49 Type I 58
13 b 24 June 2013 Male Type I 49 Type I 55
14 c 24 June 2013 Male Type I 48 Type I ―
15 c 20 November 2013 Female Type II 47 Type II ―
16 c 20 November 2013 Male Type II 46 Type II ―
17 c 20 November 2013 Female Type II 45 Type II ―
18 c 20 November 2013 Male Type II 47 Type II ―
19 c 20 November 2013 Male Type II 47 Type II ―
20 d 24 June 2013 Male Type I 48 Type I ―
21 d 24 June 2013 Female Type II 47 Type II ―
22 e 24 June 2013 Female Type II 47 Type II ―
23 e 24 June 2013 Male Type II 47 Type II ―
24 e 24 June 2013 Male Type II 48 Type II ―
25 e 24 June 2013 Male Type II 48 Type II ―
26 e 20 November 2013 Female Type II 48 Type II ―
27 e 20 November 2013 Female Type II 48 Type II ―
28 e 20 November 2013 Female Type II 46 Type II ―
29 e 20 November 2013 Male Type II 47 Type II ―
30 f 24 June 2013 Female Type II 47 Type II ―
31 f 24 June 2013 Female Type II 48 Type II ―
32 f 24 June 2013 Male Type II 48 Type II ―
33 f 24 June 2013 Female Type II 47 Type II ―
34 f 24 June 2013 Female Type II 45 Type II ―
35 f 24 June 2013 Male Type II 48 Type II ―
36 g 20 November 2013 Male Type II 47 Type II 93
37 g 20 November 2013 Male Type II 45 Type II ―
38 g 20 November 2013 Male Type II 46 Type II ―
39 g 20 November 2013 Female Type II 45 Type II 99
40 g 20 November 2013 Female Type I 51 Type I 59
41 g 20 November 2013 Male Type II 45 Type II ―
42 g 20 November 2013 Male Type II 46 Type II 90
43 g 20 November 2013 Male Type II 45 Type II ―
44 g 20 November 2013 Male Type II 44 Type II ―
45 g 20 November 2013 Male Type I 49 Type I 55
46 g 20 November 2013 Male Type I 48 Type I 53
47 h 20 November 2013 Male Type II 46 Type II ―
48 i 20 November 2013 Female Type I 49 Type I ―
49 ― 24 May 2015 Male Type I ― Type I 55
50 ― 24 May 2015 Female Type I ― Type I 55
51 ― 24 May 2015 Female Type I ― Type I 55
52 ― 24 May 2015 Female Type II ― Type II 99
53 ― 24 May 2015 Male Type II ― Type II 99
54 ― 24 May 2015 Male Type II ― Type II 99
Table 2 Comparison of the characteristics of each dojo loach specimen collected from the Naka-ikemi Wetland, Fukui Prefecture, Japan.
Specimen no.
Date of collection
No. in Fig. 4
Standard
length (mm) Sex mtDNA
lineages
1 April 17, 2016 St. 1 47 Female Type II
2 April 17, 2016 St. 1 51 Male Type II
3 April 17, 2016 St. 1 48 Female Type II
4 April 17, 2016 St. 1 61 Male Type II
5 April 17, 2016 St. 1 49 Female Type II
6 April 17, 2016 St. 1 65 Male Type II
7 April 17, 2016 St. 1 49 Male Type II
8 April 17, 2016 St. 1 79 Female Type II
9 April 17, 2016 St. 1 70 Male Type II
10 April 17, 2016 St. 1 60 Male Type II
11 April 17, 2016 St. 1 66 Male Type II
12 April 17, 2016 St. 1 71 Male Type II
13 April 17, 2016 St. 1 88 Male Type II
14 April 17, 2016 St. 1 64 Male Type II
15 April 17, 2016 St. 1 51 Female Type II
16 April 17, 2016 St. 1 48 Female Type II
17 April 17, 2016 St. 1 43 Female Type II
18 April 17, 2016 St. 1 49 Female Type II
19 April 17, 2016 St. 1 67 Female Type II
20 April 17, 2016 St. 1 32 Female Type II
21 April 17, 2016 St. 1 62 Female Type II
22 April 17, 2016 St. 1 44 Female Type II
23 April 17, 2016 St. 1 45 Female Type II
24 April 17, 2016 St. 1 57 Female Type II
25 April 17, 2016 St. 1 50 Male Type II
26 April 17, 2016 St. 1 112 Female Type II
27 April 17, 2016 St. 1 49 Male Type II
28 April 17, 2016 St. 1 40 Female Type II
29 April 17, 2016 St. 1 56 Male Type II
30 April 17, 2016 St. 1 45 Female Type II
31 April 17, 2016 St. 1 53 Female Type II
32 April 17, 2016 St. 2 55 Male Type II
33 April 17, 2016 St. 2 31 Unknown Type II
34 April 17, 2016 St. 2 34 Unknown Type I
35 April 17, 2016 St. 2 49 Unknown Type II
36 April 17, 2016 St. 2 35 Unknown Type II
37 April 17, 2016 St. 2 35 Unknown Type II
38 April 17, 2016 St. 2 35 Unknown Type I
39 April 17, 2016 St. 2 35 Unknown Type II
40 April 17, 2016 St. 2 80 Female Type II
41 April 17, 2016 St. 2 77 Female Type II
42 April 17, 2016 St. 2 78 Female Type II
43 April 17, 2016 St. 2 94 Female Type II
44 April 17, 2016 St. 3 35 Unknown Type I
45 April 17, 2016 St. 3 80 Male Type II
46 May 30, 2016 St. 1 48 Female Type II
47 May 30, 2016 St. 1 35 Unknown Type II
48 May 30, 2016 St. 1 40 Male Type II
49 May 30, 2016 St. 1 51 Male Type II
50 May 30, 2016 St. 1 76 Female Type II
51 May 30, 2016 St. 1 35 Unknown Type II
52 May 30, 2016 St. 1 49 Male Type II
53 May 30, 2016 St. 1 35 Unknown Type II
54 May 30, 2016 St. 1 81 Female Type II
55 May 30, 2016 St. 1 81 Male Type II
56 May 30, 2016 St. 1 118 Male Type II
57 May 30, 2016 St. 1 81 Female Type II
58 May 30, 2016 St. 1 81 Male Type II
59 May 30, 2016 St. 1 81 Female Type II
60 May 30, 2016 St. 1 130 Female Type II
61 May 30, 2016 St. 1 100 Female Type II
62 May 30, 2016 St. 2 35 Unknown Type II
63 May 30, 2016 St. 2 35 Unknown Type II
64 May 30, 2016 St. 2 58 Female Type II
65 May 30, 2016 St. 2 65 Male Type II
66 May 30, 2016 St. 2 71 Female Type II
67 May 30, 2016 St. 2 72 Female Type II
68 May 30, 2016 St. 2 58 Female Type II
69 May 30, 2016 St. 2 58 Unknown Type II
70 May 30, 2016 St. 2 56 Female Type II
71 May 30, 2016 St. 2 35 Unknown Type II
72 May 30, 2016 St. 2 35 Unknown Type I
73 May 30, 2016 St. 2 62 Unknown Type I
74 May 30, 2016 St. 2 58 Male Type II
75 May 30, 2016 St. 2 97 Female Type II
76 May 30, 2016 St. 2 81 Male Type II
77 May 30, 2016 St. 2 81 Male Type I
78 May 30, 2016 St. 2 78 Female Type II
79 July 3, 2016 St. 1 24 Unknown Type II
80 July 3, 2016 St. 1 47 Male Type II
81 July 3, 2016 St. 1 58 Male Type II
82 July 3, 2016 St. 1 56 Female Type II
83 July 3, 2016 St. 1 70 Male Type II
84 July 3, 2016 St. 1 77 Female Type II
85 July 3, 2016 St. 1 81 Female Type II
86 July 3, 2016 St. 1 90 Male Type II
87 July 3, 2016 St. 1 78 Male Type II
88 July 3, 2016 St. 1 92 Male Type II
89 July 3, 2016 St. 1 112 Female Type II
90 July 3, 2016 St. 1 121 Female Type II
91 July 3, 2016 St. 2 64 Female Type I
92 July 3, 2016 St. 2 28 Unknown Type II
93 July 3, 2016 St. 2 32 Unknown Type II
94 July 3, 2016 St. 2 33 Unknown Type II
95 July 3, 2016 St. 2 29 Unknown Type II
96 July 3, 2016 St. 2 34 Unknown Type II
97 July 3, 2016 St. 2 26 Unknown Type II
98 July 3, 2016 St. 2 44 Unknown Type I
99 July 3, 2016 St. 2 33 Unknown Type II
100 July 3, 2016 St. 3 83 Female Type I
101 July 3, 2016 St. 3 84 Male Type I
102 July 3, 2016 St. 3 96 Female Type I
103 August 14, 2016 St. 1 35 Unknown Type II
104 August 14, 2016 St. 1 35 Unknown Type II
105 August 14, 2016 St. 1 103 Female Type II
106 August 14, 2016 St. 1 110 Male Type II
107 August 14, 2016 St. 1 81 Male Type II
108 August 14, 2016 St. 1 53 Female Type II
109 August 14, 2016 St. 1 115 Male Type II
110 August 14, 2016 St. 1 53 Female Type II
111 August 14, 2016 St. 1 110 Male Type II
112 August 14, 2016 St. 1 118 Male Type II
113 August 14, 2016 St. 1 80 Female Type II
114 August 14, 2016 St. 1 115 Female Type II
115 August 14, 2016 St. 1 133 Male Type II
116 August 14, 2016 St. 1 98 Female Type II
117 August 14, 2016 St. 1 106 Male Type II
118 August 14, 2016 St. 1 129 Female Type II
119 August 14, 2016 St. 2 33 Unknown Type II
120 August 14, 2016 St. 2 40 Male Type II
121 August 14, 2016 St. 2 37 Unknown Type II
122 August 14, 2016 St. 3 102 Female Type II
123 August 14, 2016 St. 3 70 Male Type II
124 August 14, 2016 St. 3 52 Female Type II
125 August 14, 2016 St. 3 97 Female Type I
126 August 14, 2016 St. 3 114 Male Type I
127 August 14, 2016 St. 3 103 Male Type II
128 August 14, 2016 St. 3 94 Male Type I
129 August 14, 2016 St. 3 108 Male Type I
130 August 14, 2016 St. 3 110 Female Type I
131 October 23, 2016 St. 3 ― ― Type I
132 October 23, 2016 St. 3 ― ― Type I
133 October 23, 2016 St. 3 ― ― Type I
134 October 23, 2016 St. 3 ― ― Type I
135 October 23, 2016 St. 3 ― ― Type I
136 October 23, 2016 St. 3 ― ― Type I
137 October 23, 2016 St. 3 ― ― Type I
138 October 23, 2016 St. 3 ― ― Type I
139 October 23, 2016 St. 3 ― ― Type I
140 October 23, 2016 St. 3 ― ― Type II
141 July 17, 2017 St. 3 ― ― Type I
142 July 17, 2017 St. 3 ― ― Type I
143 July 17, 2017 St. 3 ― ― Type I
144 July 17, 2017 St. 3 ― ― Type I
145 July 17, 2017 St. 3 ― ― Type I
146 July 17, 2017 St. 3 ― ― Type I
147 July 17, 2017 St. 3 ― ― Type I
148 July 17, 2017 St. 3 ― ― Type I
149 July 17, 2017 St. 3 ― ― Type I
150 July 17, 2017 St. 3 ― ― Type II
Table 3 Sampling locations, number of individuals and Accession number of Misgurnus anguillicaudatus and its related species, used in the phylogenetic analyses.
NO. No. in Fig. 7
Locality
N Accession No.
Prefecture/Country City, Town, Lake or River
1 1 Hokkaido Kutcharo Lake 8 ―
2 2 Nayoro 2 ―
3 ― AB473261
4 ― AB473262
5 ― AB473263
6 3 Memanbetsu 4 ―
7 4 Abashiri 15 ―
8 5 Kotanbetsu 1 ―
9 Furano ― AB473266
10 ― AB473267
11 6 Hokuryu 6 ―
12 7 Sorachi District 4 ―
13 8 Monbetsu 3 ―
14 Atsuma ― AB473279
15 ― AB473267
16 Mukawa ― AB473267
17 Kyowa ― AB473276
18 ― AB473277
19 ― AB473267
20 Imakane ― AB473274
21 ― AB473275
22 ― AB473267
Misgurnus anguillicaudatus
23 9 Jyunsai Lake 31
24 10 Hokkaido Onuma Lake 18
25 Aomori Hirakawa ― AB473266
26 Towada ― AB473274
27 ― AB473267
28 Fujisaki ― AB473266
29 Goshogawara ― AB473277
30 ― AB473281
31 Tohoku ― AB473292
32 11 Yokohama 11 ―
33 12 Shichinohe 3 ―
34 Iwate Oshu ― AB473277
35 Hanamaki ― AB473267
36 Miyagi Yamamoto ― AB473301
37 ― AB473274
38 Imaizumi ― EF508556
39 ― EF508557
40 Natori ― AB473266
41 ― AB473267
42 13 8
43 14 Toyosato 7
44 Akita Hago ― AB473281
45 Kazuno ― AB473267
46 Yokote ― AB473266
47 Yamagata Tsuruoka ― AB473281
48 Fukushima Yabuki ― AB473266
49 Sugakawa ― AB473308
50 Fukushima Sugakawa ― AB473309
51 Ibaraki Kashima ― AB614357
52 Tochigi Otawara ― AB473281
53 Sakura ― AB473312
54 ― AB473313
55 ― AB473314
56 Gunma Kawaba ― AB473317
57 Saitama Hanno ― AB473279
58 Chiba Minamiboso ― AB473279
59 Niigata Niigata ― AB473266
60 ― AB473281
61 ― AB473279
62 ― AB473288
63 Nagaoka ― AB473267
64 15 Sado 4 ―
65 16 Tokamachi 11
66 Toyama Himi ― AB473325
67 17 Ishikawa Wajima 42 ―
68 18 Anamizu 20 ―
69 19 Nagasaki 9 ―
70 20 Nozaki 8 ―
71 21 Neyamu 1 ―
72 22 Kouda 29 ―
73 23 Enome 4 ―
74 24 Hannoura 8 ―
75 ― AB599978
76 Ishikawa Nanao ― AB614359
77 25 Shinbo 21 ―
78 26 Akaura 3 ―
79 Hodatsu ― AB473274
80 ― AB473331
81 ― AB473333
82 Kanazawa ― AB473288
83 ― AB473326
84 ― AB473329
85 Komatsu ― AB473330
86 27 Fukui Tsuruga, Naka-ikemi Wetland 12 ―
87 Tsuruga, Ikenokouchi 1 ―
88 28 Fukui 3 ―
89 Echizen ― AB898249
90 29 1 ―
91 ― AB473266
92 ― AB473336
93 30 Kurokawa 5 ―
94 31 Obama 1 ―
95 ― AB899674
96 ― AB899684
97 Katsuyama ― AB473334
98 32 1 ―
99 Aichi Owariasahi ― AB473266
100 Yatomi ― AB473279
101 33 Anjo 3 ―
102 34 Aichi Inuyama 1 ―
103 35 Nagoya 1 ―
104 Gifu Gujo ― AB473301
105 36 Kiso River 1 ―
106 37 Kaizucho 3 ―
107 Shiga Azuchi ― AB473281
108 Higashiomi ― AB473301
109 ― AB473350
110 Kyoto Kyotango ― AB473301
111 38 9 ―
112 39 Miyazu 2 ―
113 40 Maizuru 1 ―
114 Nara Yamazoe ― AB473359
115 41 Kashihara 2 ―
116 Hyogo Kato ― AB473267
117 Wakayama Minabe ― AB473362
118 ― AB473363
119 ― AB473365
120 42 Kujinokawa 1 ―
121 Tottori Oyama ― AB473368
122 Okayama Kasaoka ― AB473267
123 43 Maniwa 3 ―
124 Yamaguchi Ube ― AB473383
125 Hagi ― AB473384
126 Hofu ― AB473386
127 Iwaguni ― AB614358
128 44 Yamaguchi Tawarayama 1 ―
129 Kagawa Takamatsu ― AB473396
130 Ehime Seiyo ― AB473398
131 Kochi Haruno ― AB473267
132 Fukuoka Fukuoka ― AB978285
133 Miyako ― AB473400
134 Saga Yoshinogari ― AB473401
135 Nagasaki Tsushima ― AB473402
136 Miyazaki Ebino ― AB473406
137 45 Kagoshima Ichikikushikino 2 ―
138 China Hejiang ― AY625700
139 Russia Sakhalin ― JN858856
140 ― JN858857
141 ― JN858858
142 ― JN858859
143 ― AB473261
144 ― AB473277
145 ― AB473308
146 ― EF508557
147 Amur River ― JN858854
148 Lotos Lake ― JN858860
149 Poland Warmia-Masuria ― EF508561
Misgurnus nikolskyi
Misgurnus fossilis
Misgurnus dabryanus
150 China Huangshan ― AY625701
151 Russia Amur River ― EF508565
152 47 Kyoro Maizuru 1 ―
153 Korea ― EU670767
154 Russia Amur River ― JN858850
155 Korea ― EU508498
156 Germany Niedersachsen ― EU508508
157 Slovakia Presov ― AY887851
158 India West Bengal ― EU508583
Misgurnus mohoity
Cobitis lutheri
Cobitis taenia
OutGroup
Sabanejewia balcanica
Pangio pangia
Table 4. The list summarizes the results of D-loop Haplotypes, Nuclear DNA linages and phylogeny Groups.
No. mtDNA linages
D-loop Haplotypes
Nuclear DNA
linages Groups N
1 Type I Haplotype III ― Group 1 1
Haplotype I Type I / Type I Group 3 1 Haplotype II Type I / Type I Group 3 6
2 Type I ― ― Group 1 2
3 Type I ― ― Group 3 ―
4 Type I ― ― Group 3 ―
5 Type I ― ― Group 3 ―
6 Type I ― ― Group 1 2
― ― Group 3 2
7 Type I Haplotype I Type I / Type I Group 3 4
Haplotype II Type I / Type I Group 3 11
8 Type I ― ― Group 1 1
9 Type II ― ― Japanese native ―
10 Type II ― ― Japanese native ―
11 Type I Haplotype III ― Group 1 3
Type II Haplotype V ― Japanese native 3
12 Type II ― ― Japanese native 4
13 Type II ― ― Japanese native 3
14 Type II ― ― Japanese native ―
15 Type II ― ― Japanese native ―
16 Type II ― ― Japanese native ―
17 Type I ― ― Group 3 ―
Misgurnus anguillicaudatus
18 Type II ― ― Japanese native ―
19 Type II ― ― Japanese native ―
20 Type II ― ― Japanese native ―
21 Type I ― ― Group 3 ―
22 Type II ― ― Japanese native ―
23 Type I Haplotype III Type I / Type II Group 1 11
Type II ― Type II / Type II Japanese native 20
24 Type I Haplotype III Type I / Type II Group 1 7
Type II ― Type II / Type II Japanese native 11
25 Type II ― ― Japanese native ―
26 Type II ― ― Japanese native ―
27 Type II ― ― Japanese native ―
28 Type II ― ― Japanese native ―
29 Type I ― ― Group 1 ―
30 Type II ― ― Japanese native ―
31 Type II ― ― Japanese native ―
32 Type II ― ― Japanese native 11
33 Type II ― ― Japanese native 3
34 Type I ― ― Group 1 ―
35 Type II ― ― Japanese native ―
36 Type II ― ― Japanese native ―
37 Type II ― ― Japanese native ―
38 Type II ― ― Asian continent origin ―
39 Type I ― ― Group 4 ―
40 Type II ― ― Japanese native ―
41 Type II ― ― Japanese native ―
42 Type II ― ― Japanese native 8
43 Type II ― ― Japanese native 7
44 Type II ― ― Japanese native ―
45 Type II ― ― Japanese native ―
46 Type II ― ― Japanese native ―
47 Type II ― ― Japanese native ―
48 Type II ― ― Japanese native ―
49 Type II ― ― Japanese native ―
50 Type II ― ― Japanese native ―
51 Type II ― ― Asian continent origin ―
52 Type II ― ― Japanese native ―
53 Type I ― ― Group 6 ―
54 Type I ― ― Group 6 ―
55 Type I ― ― Group 6 ―
56 Type II ― ― Asian continent origin ―
57 Type II ― ― Asian continent origin ―
58 Type II ― ― Asian continent origin ―
59 Type II ― ― Japanese native ―
60 Type II ― ― Japanese native ―
61 Type II ― ― Asian continent origin ―
62 Type II ― ― Japanese native ―
63 Type II ― ― Japanese native ―
64 Type II ― ― Japanese native 4
65 Type II ― ― Japanese native 11
66 Type II ― ― Japanese native ―
67 Type I Hap A ― Group 1 4
Type II Hap B Type II / Type II Japanese native 38
68 Type II ― ― Japanese native 20
69 Type II ― ― Japanese native 9
70 Type II ― ― Japanese native 8
71 Type II ― ― Japanese native 1
72 Type I Hap A Type I / Type II Group 1 4
Type II ― Type II / Type II Japanese native 25
73 Type II ― ― Japanese native 4
74 Type II ― ― Japanese native 8
75 Type II ― ― Japanese native ―
76 Type I ― ― Group 1 ―
77 Type I Haplotype XI Type I / Type I Group 6 20
Type II Hap C Type II / Type II Japanese native 1
78 Type II ― ― Japanese native 3
79 Type II ― ― Japanese native ―
80 Type II ― ― Japanese native ―
81 Type II ― ― Japanese native ―
82 Type II ― ― Japanese native ―
83 Type II ― ― Japanese native ―
84 Type II ― ― Japanese native ―
85 Type II ― ― Japanese native ―
86 Type I Hap D Type I / Type I Group 2 6
Type II Hap B Type II / Type II Japanese native 6
87 Type I ― ― Group 2 1
88 Type II Haplotype VII ― Asian continent origin 3
89 Type I ― ― Group 1 ―
90 Type II ― ― Japanese native 1
91 Type II ― ― Japanese native ―
92 Type II ― ― Japanese native ―
93 Type II ― ― Asian continent origin 5
94 Type II ― ― Japanese native 1
95 Type II ― ― Japanese native ―
96 Type II ― ― Japanese native ―
97 Type II ― ― Japanese native ―
98 Type II ― ― Japanese native 1
99 Type II ― ― Japanese native ―
100 Type II ― ― Asian continent origin ―
101 Type II ― ― Asian continent origin 3
102 Type II ― ― Japanese native 1
103 Type II ― ― Japanese native 1
104 Type II ― ― Japanese native ―
105 Type II ― ― Japanese native 1
106 Type II ― ― Japanese native 3
107 Type II ― ― Japanese native ―
108 Type II ― ― Japanese native ―
109 Type II ― ― Japanese native ―
110 Type II ― ― Japanese native ―
111 Type II ― ― Japanese native 9
112 Type II ― ― Japanese native 2
113 Type II ― ― Asian continent origin 1
114 Type II ― ― Asian continent origin ―
115 Type II Haplotype VII ― Asian continent origin 2
116 Type II ― ― Japanese native ―
117 Type II ― ― Japanese native ―
118 Type II ― ― Japanese native ―
119 Type II ― ― Japanese native ―
120 Type II ― ― Japanese native 1
121 Type II ― ― Japanese native ―
122 Type II ― ― Japanese native ―
123 Type II ― ― Japanese native 3
124 Type II ― ― Japanese native ―
125 Type II ― ― Japanese native ―
126 Type II ― ― Asian continent origin ―
127 Type II ― ― Japanese native ―
128 Type II ― ― Japanese native 1
129 Type II ― ― Japanese native ―
130 Type II ― ― Japanese native ―
131 Type II ― ― Japanese native ―
132 Type II ― ― Japanese native ―
133 Type II ― ― Japanese native ―
134 Type II ― ― Japanese native ―
135 Type II ― ― Japanese native ―
136 Type II ― ― Japanese native ―
137 Type II ― ― Japanese native 2
138 Type II ― ― Asian continent origin ―
139 Type I ― ― Group 1 ―
140 Type I ― ― Group 1 ―
Misgurnus nikolskyi
141 Type I ― ― Group 1 ―
142 Type I ― ― Group 3 ―
143 Type I ― ― Group 3 ―
144 Type I ― ― Group 1 ―
145 Type I ― ― Group 5 ―
146 Type I ― ― Group 4 ―
147 ― ― ― ― ―
148 ― ― ― ― ―
149 ― ― ― ― ―
150 ― ― ― ― ―
151 ― ― ― ― ―
152 ― ― ― ― 1
153 ― ― ― ― ―
154 ― ― ― ― ―
155 ― ― ― ― ―
156 ― ― ― ― ―
157 ― ― ― ― ―
158 ― ― ― ― ―
Misgurnus fossilis
Misgurnus dabryanus
Misgurnus mohoity
Cobitis lutheri
Cobitis taenis
OutGroup
Sabanejewia balcanica
Pangio pangia
Table 5 Sampling locations, mtDNA linages, D-loop Haplotypes and number of individuals of Misgurnus anguillicaudatus, used in the phylogenetic analyses.
NO. No. in Fig. 7
Locality mtDNA
linages
D-loop
Haplotypes N Prefecture/Country City, Town, Lake or River
1 1 Hokkaido Kutcharo Lake Type I Haplotype I 6
Type I Haplotype II 1 Type I Haplotype III 1
7 4 Abashiri Type I Haplotype I 4
Type I Haplotype II 2
11 6 Hokuryu Type I Haplotype III 3
Type II Haplotype V 2
23 9 Jyunsai Lake Type I Haplotype III 1
24 10 Onuma Lake Type I Haplotype III 2
67 17 Ishikawa Wajima Type I Hap A 1
Type II Hap B 2
72 22 Kouda Type I Haplotype III 1
Type I Hap A 1
77 25 Shinbo Type I Haplotype XI 4
Type II Hap C 1
86 27 Fukui Tsuruga, Naka-ikemi Wetland Type I Hap D 6
Type II Hap B 6
88 28 Fukui Type II Haplotype VII 1
115 41 Nara Kashihara Type II Haplotype VII 2
Misgurnus anguillicaudatus
Table 6 Species and DDBJ accession numbers of DNA sequences used in the phylogenetic analyses of D-loop.
Species Haplotypes Accession number
Misgurnus anguillicaudatus Haplotype I AB306717
AB306721 AB306723
Haplotype II AB306724
AB306725
Haplotype III AB306729
AB306730
Haplotype IV AB306731
Haplotype V AB306733
Haplotype VII AB306762
Haplotype VIII AB306783
Haplotype X AB306786
Haplotype XI AB306789
AB306790
Haplotype XII AB306791
Misgurnus nikolskyi AB242171
Misgurnus dabryanus Hap 1 LC025649
Misgurnus mohoity Hap 5 LC025653
Cobitis striata striata AB054125