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1) T. Michinobu, M. Bito, Y. Yamada, Y. Katayama, K.

Noguchi, E. Masai, M. Nakamura, S. Ohara, K. Shige- hara,Bull. Chem. Soc. Jpn.,80, 2436῎2442 (2007).

2) IJ‚ƒK„…L=†‡ˆMN45, 239῎246 (2007).

3) T. Michinobu, M. Hishida, M. Sato, Y. Katayama, E.

Masai, M. Nakamura, Y. Otsuka, S. Ohara, K. Shigehara, Polym. J.,40, 68῎75 (2008).

4) T. Michinobu, Y. Inazawa, K. Hiraki, Y. Katayama, E.

Masai, M. Nakamura, S. Ohara, K. Shigehara, Chem.

Lett.,37, 154῎155 (2008).

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Table 2

DHA High-LA 9.9

high-ALA 16.7 1.7 n-6/

n-3 !" High-LA 1.2 high-ALA 0.7 #

BDNF $% Fig. 1 High-LA

74 pg/g striatum High-ALA 124 pg/g striatum &'() 1.6 %#

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1) Miyazawa D.,et al.,Life Sci.,73, 2083῍2090 (2003).

2) Ikemoto A.,et al.,J. Lipid Res.,42, 1655῍1663 (2001).

Table 1. Fatty acid composition of dietary oils.

Fatty acid Sa%ower oil Perilla oil

16 : 0 6.7 3.1

18 : 0 2.4 1.9

18 : 1 16.1 16.8

18 : 2 n-6 73.9 15.7

18 : 3 n-3 0.4 59.2

20 : 0 0.3 0.1

20 : 1 0.2 0.2

n-6/n-3 168.0 0.3

Values represent the percentage of total fatty acids.

Table 2. Fatty acid composition of striatal total lipids.

Fatty acid (FA) High-LA High-ALA

of total fatty acids (w/w) Saturated FA

14 : 0 0.10.0 0.10.0

16 : 0 DMA 2.80.2 2.20.4 ῎

16 : 0 21.52.4 20.41.1

18 : 0 DMA 4.30.4 3.60.8

18 : 0 24.33.2 21.70.8

20 : 0 0.40.1 0.30.1

22 : 0 0.50.2 0.30.1

24 : 0 0.70.3 0.50.1

Monounsaturated FA 14 : 1

16 : 1 0.40.1 0.40.0

18 : 1 DMA 1.40.4 1.20.2

18 : 1 18.11.1 17.71.1

20 : 1 1.50.5 1.20.2

22 : 1 0.10.0 0.10.1

24 : 1 1.80.7 1.40.3

N-6 polyunsaturated FA

18 : 2 n-6 0.50.1 0.50.1

20 : 3 n-6 0.30.0 0.50.0 ῎

20 : 4 n-6 8.00.4 8.50.4

22 : 4 n-6 2.40.4 2.10.4

22 : 5 n-6 1.00.2 0.20.0 ῎

N-3 polyunsaturated FA

18 : 3 n-3 Tr Tr

22 : 5 n-3 0.10.1 0.40.3

22 : 6 n-3 9.80.9 16.71.4 ῎

n-6/n-3 1.20.0 0.70.1 ῎

Total FA (mg/g of brain) 26.34.2 30.12.8 Values represent meansSD, n4. An asterisk (῎) in- dicates significant di#erence between the two dietary groups (P‘0.05 by Student’sttest). DMA, dimethylace- tal derived from plasmalogen; Tr, trace (‘0.1).

Fig 1. BDNF content of in weeks old mice striatum.

BDNF content in mice fed high-LA or High-ALA diet were determined as described in the text.

Values represent meansSD for 4 mice. An asterisk (῎) indicates significant di#erence between the two dietary groups (P‘0.005 by Student’sttest)

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