酸化還元酵素
第1群
S Cys
H NAD
+NH N
His
HC C O
-CH
2OPO
32-OH
S Cys NAD
+NH N
+His H H
HC
CH
2OPO
32-NADH OH
HC C
H O
CH
2OPO
32-OH
HC C
CH
2OPO
32-OH
OPO
32-glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.9)
NADH
NAD
+Pi
O
HC C O CH
2OPO
32-OH
S Cys NAD
+NH N
+His H
-
O P O
OH O
-HC C
H O
CH
2OPO
32-OH
S Cys
H NAD
+NH N
His
NAD: nicotinamide adenine dinucleotide
O O
N + O
OH OH
H H
H H
O P O
C O
O
N N N
N
NH 2
O O OH
H H
H H
O P
NH 2
R
+H + N
C NH 2 H O
H
+2H -2H
NAD：補酵素 ... 体内に最も多量に存在
発見 1904 by A. Harden & W. J. Young 構造決定 1935 by F. Schlenk & H. von Euler NADP：還元剤
発見 1934 by O. Warburg & W. Christian R = H → NAD
R = phosphate → NADP
nicotinamide adenine dinucleotide phosphate
O
S Cys
H NAD +
NH N
His
HC C
H O
CH 2 OPO 3 2- OH
HC C
H O
CH 2 OPO 3 2- OH
S H NAD +
NH
N
HC
C O -
CH 2 OPO 3 2- OH
S Cys NAD +
NH N +
His H
H HC
C
H O
CH 2 OPO 3 2- OH
S Cys
H NAD +
NH N
His
HC
C O
CH 2 OPO 3 2- OH
S NADH
NH N +
H
HC
C O -
CH 2 OPO 3 2- OH
S Cys NAD +
NH N +
His H
H
HC
C O
CH 2 OPO 3 2- OH
S Cys NADH
NH N +
His H
NADH
NAD + Pi
HC
C O
CH 2 OPO 3 2- OH
S Cys NAD +
NH N +
His H
- O P
O
OH
O -
S Cys
H NAD +
NH N
His
HC C
CH 2 OPO 3 2- OH
OPO 3 2- O
HC
C O
CH 2 OPO 3 2- OH
S NAD +
NH N +
H
- O P
O
OH
O -
電子伝達系・酸化的リン酸化
NAD: nicotinamide adenine dinucleotide
O O
N + O
OH OH
H H
H H
O P O
C O
O
N N N
N
NH 2
O O OH
H H
H H
O P
NH 2
+H + N
C NH 2 H O
H
+2H -2H
NAD：補酵素 ... 体内に最も多量に存在
発見 1904 by A. Harden & W. J. Young 構造決定 1935 by F. Schlenk & H. von Euler NADP：還元剤
発見 1934 by O. Warburg & W. Christian R = H → NAD
R = phosphate → NADP
nicotinamide adenine dinucleotide phosphate
O
C H
2CH
3O
CH
3O
CH
3O
O
C H C CH
3H
2C H
n CoQ: ubiquinone
n=10 高等動物
n=6 酵母
CH2CH3OCH3OCH3OOCHCCH3CH2HNAD+FMNH2FMNFe2+-SFe3+-SCoQH2ATP2H+nCoQ: ubiquinone n=10 高等動物 n=6 酵母NADH + H+CoQ + 2H+ADP + Pi複合体Ⅰ2e-2e- CH2CH3OCH3OCH3OOCHCCH3CH2HNAD+FMNH2FMNFe2+-SFe3+-SCoQH2ATP2H+nCoQ: ubiquinone n=10 高等動物 n=6 酵母NADH + H+CoQ + 2H+ADP + Pi複合体Ⅰ2e-2e-
vitamin C (L-ascorbic acid 、 L- アルコルビン酸 )
カルボン酸ではないのに、なぜ『酸』？
HO O
HO OH
O OH （環状）エステル
ラクトン
O
HO OH
HO O
OH
O
HO + OH
O - HO
OH
O O
酸化 還元
L-アスコルビン酸 還元型VC
このHがはずれ易い pKa=4.17
