小括

62

63

第4章 総括

本研究では、iBMECsへの分化誘導時の基底膜成分としてLN221Fを用いるこ とで、高いバリア機能を長期間維持する iBMECs を簡便に作製できることを明 らかにした。さらに、本研究では、剥離液を2段階で処理することにより、iBMECs の前駆細胞であるiEPCsを簡便に純化可能であることを示した。また、ROCK阻

害剤、TGF-β受容体阻害剤、およびGSK3β阻害剤を培地に加えることで、EPCs

としての性質を維持しつつ拡大培養が可能であることも明らかにした。これら の知見はヒトBBBにおける薬物動態や病態解析の研究に有用なiBMECsの作製 やヒト BBB の発生および分化機構の解明に大きく寄与することが予想される。

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謝辞

本研究の遂行に際し、終始御懇篤な御指導、御鞭撻を賜り、また本論文の御校 閲を頂きました名古屋市立大学大学院薬学研究科臨床薬学分野 松永民秀 教授 に深甚なる謝意を表します。

本研究の遂行に際し、格別の御配慮と御激励を賜りました名古屋市立大学大 学院薬学研究科臨床薬学分野 鈴木匡 教授に深甚なる謝意を表します。

本論文作成にあたり、種々の有益な御助言と御校閲を賜りました、名古屋市立 大学大学院薬学研究科細胞分子薬効解析学分野 山村寿男 教授、名古屋市立大 学大学院薬学研究科神経薬理学分野 粂和彦 教授ならびに名古屋市立大学大学 院薬学研究科病態解析学分野 青山峰芳 教授に深謝いたします。

本研究の遂行に際し、親身なる御指導、有益な御助言を賜り、バイオインフォ マティクス解析技術をご提供頂きました名古屋市立大学大学院薬学研究科臨床 薬学分野 星野真一 教授ならびに尾上耕一 助教に謹んで御礼申し上げます。

本研究の遂行に際し、有益な御助言とご協力を賜りました名古屋市立大学大 学院薬学研究科臨床薬学分野 岩尾岳洋 准教授ならびに菊池千草 講師に厚く 御礼申し上げます。

本研究の遂行に際し、終始御懇篤な御指導、御鞭撻を賜り、また本論文の御校 閲を頂きました名古屋市立大学大学院薬学研究科臨床薬学分野 坡下真大 講師

65

に深甚なる謝意を表します。

本研究を遂行するにあたり、hiPSCs をご供与頂きました国立成育医療研究セ ンター研究所 阿久津英憲 博士、宮川世志幸 博士、大喜多肇 博士、清河信敬 博 士、豊田雅士 博士ならびに梅澤明弘 博士に謹んで御礼申し上げます。

研究途上、種々の御便宜を図ってくださいました名古屋市立大学大学院薬学 研究科臨床薬学分野ならびに薬学部臨床薬学教育研究センターの卒業生、在室 生ならびに研究員に感謝致します。

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