㻌 ⪃ᐹ

ඃ⚽䛺㞝䜻䝒䝛䛛䜙䛾⢭ᾮ䛾Ᏻᐃⓗ౪⤥䛸㑇ఏⓗከᵝᛶ䛾⥔ᣢ䜢┠ⓗ䛻ேᕤᤵ⢭ἲ䛾

☜❧䜢ヨ䜏䛯䚹

䜎䛪䠈㞝䜻䝒䝛䛛䜙┤⭠㟁Ẽ่⃭ἲ䛷⢭ᾮ᥇ྲྀ䛾᮲௳タᐃ䜢⾜䛳䛯䚹㟁Ẽ่⃭䠄࿘Ἴᩘ

60 Hz䠅䜢1 V 䛛䜙5 V䜎䛷䠄1 ⛊㏻㟁䠉1 ⛊ఇṆ䛩䜛᧯స䠅䜢5 䝃䜲䜽䝹⧞䜚㏉䛧䛯䚹䝃䜲

䜽䝹ᩘ䛸⢭ᾮ᥇ྲྀ䛾㛵㐃䜢ㄪ䜉䛯䛸䛣䜝䠈1䛸 5 䝃䜲䜽䝹䛷䛿⢭ᾮ䛿 5V䛾䜏䛷᥇ྲྀ䛥䜜䠈2 䡚4 䝃䜲䜽䝹䛷䛿3V䛛䜙5V 䛾㛫䛷⢭ᾮ䛜᥇ྲྀ䛥䜜䛯 (Fig. 10)䚹䛣䛾ཎᅉ䛸䛧䛶1 䝃䜲䜽 䝹䛷䛿㈇Ⲵ่⃭䛜▷䛟⢭ᾮ᥇ྲྀ䛻䛿୙༑ศ䛷䛒䜚䠈5 䝃䜲䜽䝹䛷䛿 2䡚4 䝃䜲䜽䝹䛷᪤䛻⢭

ᾮ䛜᥇ྲྀ䛥䜜䛶䛧䜎䛳䛯䛯䜑䛸᥎ 䛥䜜䛯䚹1䡚5䝃䜲䜽䝹䜢ᇶᮏ䠄่⃭䠅᮲௳䛸䛧䛶᥇⢭ᡂຌ

⋡䜢ồ䜑䛯䛸䛣䜝 66.2%䠄47/71 㢌䠅䛷䛒䛳䛯䚹⏝ᡭἲ [14] 䛾᥇⢭ᡂຌ⋡䠄75%䠅䛻ẚ䜉䜔 䜔ప್䛷䛒䛳䛯䛜䠈ᢏ⾡ⓗ䛺୙័䜜䛥䠄┤⭠ෆ䜈䛾䝥䝻䞊䝤䛾ᤄධ῝ᗘ䛾ኻᐅ䠅䛸㞝䜻䝒䝛 䛾ᛶⓗᮍᡂ⇍䛜୍㒊ཎᅉ䛸䛧䛶⪃䛘䜙䜜䛯䚹1䡚5 䝃䜲䜽䝹ୗ䛻䛚䛡䜛ྛ㟁ᅽ1䡚5 V 䛻䛚 䛡䜛᥇ྲྀ㢌ᩘ䛸᥇⢭ᡂຌ⋡䜢㞟ィ䛧䛯䛸䛣䜝䠈᥇ྲྀ㢌ᩘ㡰఩䛿 5 V䛷22 㢌䠄᥇⢭ᡂຌ⋡䠖 31.0%䠅䠚 4 V 䛷19㢌䠄26.8% 䠚 3 V 䛷6㢌䠄8.5%䠅 䠚 2 V௨ୗ䛷0 㢌䠄0%䠅䛾㡰䛷䛒 䛳䛯䚹⢭ᾮ୰䛾⢭Ꮚᩘ㡰఩䛿㏫䛻3 V䛷62,900୓ 䠚 4 V䛷55,500୓ 䠚 5 V䛷27,900

33

୓䛾㡰䛷䛒䛳䛯䠄Fig. 11䠅䚹⢭ᾮ㔞䠄280䡚750 μL䠅䛸⢭Ꮚ⏕Ꮡᣦᩘ䠄37.5䡚90.3䠅䛻䛿㟁ᅽ㛫 䛷ᕪ䛿ㄆ䜑䜙䜜䛺䛛䛳䛯䚹䛣䜜䜙䛾⤖ᯝ䛛䜙䠈㟁ᅽ䜢 5 V 䜎䛷ୖ䛢䜛䛸᥇⢭ᡂຌ⋡䛿㧗䜎䜛 䛜䠈⢭Ꮚᩘ䛿ῶᑡ䛧䠈ಶయ䛻䜘䛳䛶䛿ⱞ③⑕≧䜢ㄏⓎ䛩䜛䛣䛸䛜ุ᫂䛧䛯䠈䛧䛯䛜䛳䛶䠈௨ ᚋ䛾⢭ᾮ᥇ྲྀ䛻䛿2䡚4 䝃䜲䜽䝹䛷㟁ᅽ3䡚4 V 䜢᥇⏝䛩䜛䛣䛸䛻䛧䛯䚹

㟁ᅽ3䡚4 V䛷⢭ᾮ䜢ᚓ䛯㞝䜻䝒䝛25㢌䠄3 V 䛷6㻌 㢌䠈4 V 䛷19㢌䠅䛾ෆ20㢌䜢⏝䛔

䛶෾⤖⢭ᾮ⼥ゎᚋ䛾ᛶ≧䜢᳨ウ䛧䛯䚹20 㢌䛾㑅ู䛻䛿⢭Ꮚ⏕Ꮡᣦᩘ䛜㧗䛔ಶయ䜢ඃඛ䛧 䛯䚹෾⤖ಖᏑ䛿ᾮయ❅⣲୰䛷 8 ᪥䡚52 㐌㛫⾜䛳䛯䛜䠈෾⤖⼥ゎᚋ䛾⢭Ꮚ⏕Ꮡᣦᩘ (Fig.

12) 䛿ᖹᆒ 28.5% పୗ䛧䛶䛔䛯䚹䛣䛾పୗ⋡䛿䝟䞁䝎 [43] 䜔䝠䜾䝬 [20] 䛷䛾ሗ࿌䛸䜋

䜌୍⮴䛧䛶䛔䛯䚹䜎䛯䠈8᪥㛫䛸25㐌㛫䛾෾⤖⼥ゎ⢭ᾮ䛾⢭Ꮚ⏕Ꮡᣦᩘ䛻ᕪ䛿ぢ䜙䜜䛺䛛 䛳䛯䛣䛸䛛䜙䠈∵⏝⢭ᾮᕼ㔘ᾮ䜢⏝䛔䛯෾⤖⢭ᾮ䛿᭱኱ 25 㐌㛫䛿ಖᏑ䛷䛝䜛䛸⪃䛘䜙䜜䛯䚹

෾⤖䞉⼥ゎ⢭ᾮ䜢⏝䛔䛶ேᕤᤵ⢭䜢ᐇ᪋䛧䛯ᮏᐇ㦂᮲௳ୗ䛷䛾㞤䜰䜹䜻䝒䝛䛾ཷ⫾⋡

䛿 81.3䠂䠄13/16 㢌䠅䛷䛒䛳䛯䚹䛣䛾⤖ᯝ䛿䜰䜹䜻䝒䝛䛒䜛䛔䛿ள✀䛷䛒䜛㖟䜻䝒䝛䛷䛾᪤

⤖ᯝ [7, 14, 15] 䛸䜋䜌୍⮴䛧䛶䛔䛯䚹∵⏝⢭ᾮᕼ㔘ᾮ䛿䜻䝒䝛⏝⢭ᾮᕼ㔘ᾮ䛸䛿䠄䝰䝹⃰

ᗘ䛻䛿ᕪ␗䛜䛒䜛䜒䛾䛾䠅ᵓᡂᡂศ䛿䝖䝸䝇⦆⾪ᾮ䜢୺య䛸䛧䛯䜒䛾䛷䛒䜛䛣䛸䛛䜙䠈௦᭰ᕼ 㔘ᾮ䛸䛧䛶ᛂ⏝䛷䛝䛯䛸⪃䛘䜙䜜䛯䚹䜎䛯䠈㧗䛔ཷ⫾⋡䛜ᚓ䜙䜜䛯⫼ᬒ䛻䛿䠈㑅ู䛧䛯㞝䜻 䝒䝛䛿ಶయᕪ䛜ᑡ䛺䛟䠈㑇ఏⓗ䛻䜒ඃ⚽䛷䛒䛳䛯䛣䛸䛜୍ᅉ䛸⪃䛘䜙䜜䛯䚹௒ᚋ䠈ேᕤᤵ⢭

䛾㢌ᩘ䜢ቑ䜔䛧䠈䛣䜜䜙䛾᥎ 䜢᳨ド䛩䜛ᚲせ䛜䛒䜛䚹

ᮏᐇ㦂䛷䛾ேᕤᤵ⢭䛿↓㯞㓉ୗ䠈⭡ቨ䜢௓䛧Ꮚᐑ㢕⟶䜢ಖᣢ䛧䠈⢭ᾮὀධჾ䛾䝥䝻䞊 䝤䜢㢕⟶῝㒊䛻ᤄධ䛧䛶⾜䛳䛯䚹Ꮚᐑ㢕⟶䛿㠀⦾Ṫᮇ䛻䛿ᑠ䛥䛟䛛䛴⣽䛟యእ䛛䜙ゐ▱䛩 䜛䛣䛸䛿ᅔ㞴䛷䛒䜛䛜䠈Ⓨ᝟ᮇ䛻䛺䜛䛸䠈◳䛟ኴ䛟䛺䜚ゐ▱ྍ⬟䛸䛺䜛䚹ேᕤᤵ⢭䛷䛿Ꮚᐑ㢕

⟶῝㒊䛻⢭ᾮ䜢ὀධ䛩䜛䛸ཷ⫾⋡䛜ྥୖ䛩䜛䛸䛾ሗ࿌ [15] 䜒䛒䜛䚹᪂つ⪃᱌䛧䛯⢭ᾮὀ ධჾ䛿እ⟄䜔ෆ⟄䛾఩⨨䜔ゅᗘ䛛䜙䝥䝻䞊䝤ඛ➃㒊䜢ㄆ▱䛩䜛䛣䛸䛜ྍ⬟䛷㢕⟶῝㒊䜎 䛷ㄏᑟ䛷䛝䜛฼Ⅼ䛜䛒䛳䛯䚹

34 5. ᑠᣓ

䜰䜹䜻䝒䝛䛻䛚䛡䜛ேᕤᤵ⢭ἲ䜢☜❧䛩䜛䛯䜑䛻䠈䜎䛪㞝䜻䝒䝛䛛䜙䛾⢭ᾮ᥇ྲྀ䜢┤⭠

㟁Ẽ่⃭ᑕ⢭ἲ䛷᳨ウ䛧䠈䛭䛾ᚋ䠈∵⏝⢭ᾮᕼ㔘ᾮ䛜⢭ᾮ䛾ᕼ㔘ቑ㔞䞉෾⤖ಖᏑ䛻ᛂ⏝

䛷䛝䜛䛛䜢ㄪ䜉䛯䚹⢭ᾮ᥇ྲྀ䛻䛿࿘Ἴᩘ60 Hz 䛷㟁ᅽ3䡚4 V䠈2䡚4 䝃䜲䜽䝹䛷Ⰻዲ䛺⢭

ᾮ㔞䠈⢭Ꮚᩘཬ䜃⢭Ꮚ⏕Ꮡᣦᩘ䛜ᚓ䜙䜜䛯䚹∵⏝⢭ᾮᕼ㔘ᾮ䜢⏝䛔䛯෾⤖䞉⼥ゎ⢭ᾮ䛾

ཷ⫾⋡䛿 81.3䠂䛷䛒䛳䛯䚹௨ୖ䠈⢭ᾮ᥇ྲྀ䛻䛚䛡䜛㟁Ẽ่⃭᮲௳䛸∵⏝⢭ᾮᕼ㔘ᾮ䛜䜰 䜹䜻䝒䝛䛾ேᕤᤵ⢭䛻ᛂ⏝䛷䛝䜛䛣䛸䛜᫂䜙䛛䛻䛺䛳䛯䚹

35

Fig. 8. An electroejaculator and rectal ejaculatory probe with four longitudinal electrodes for the red fox ^㻌

Fig. 9. An equipment for artificial insemination of the red fox. (A) outer probe;

(B) inner probe consisting of a syringe hub, thick portion, thin portion and bending tip portion ^㻌

36 Components

Molar concentration /

unit / weight percent Extender I Extender II

Tris mM 130 130

Citrate mM 45 45

Lactose mM 40 40

Raffinose mM 50 50

Fructose mM 85 85

Penicillin G IU/L 0.6 x 10⁶ 0.6 x 10⁶

Streptomycin g/L 0.6 0.6

Egg yolk v/v 20% 20%

Glycerol v/v - 13%

Table 5. Components of the semen extenders I and II

37

0 5 10 15

1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5