2016 6
2
旅と研究
東京薬科大学薬学部
野水 基義
4
4
糖脂質関連分子の機能および構造解析
〜蛍光顕微鏡と質量分析装置を効果的に利用して〜
大阪大学大学院理学研究科
樺山 一哉
9
DNA はどこまで（化学的に）入れ換え可能か？
名古屋大学大学院工学研究科
樫田 啓
15
九州大学大学院工学研究院 特任助教
与那嶺 雄介
大阪大学大学院工学研究科 博士前期課程２年
高 靖馳
22
イリノイ大学留学体験記
鳥取大学大学院工学系研究科
稲葉 央
26
第 19 回 生命化学研究会、第 10 回 バイオ関連化学シンポジウム、第 43 回 国際核酸 化学シンポジウム（ISNAC 2016）、第 26 回 バイオ・高分子シンポジウム
受賞、異動 編集後記
- 2 -
8 9
4 I 8
6 4 1
I I
I I
I
H
4 I
4 I
I 4 9
4 4
4 I
6 6 4
I I
9 I 4 I6
I 4
4 9
6
- 3 -
I 4 4
I9 1 I 4
9 4 I
I 4 4 4 I
I 69
2016 5
- 4 -
( )
1996 20 N-
GM3
(1) GM3
(2)
GM3 GM3
GM3 IR
IR
IR
(3) GM3 IR
GM3 IR (4, 5)
(6, 7)
- 5 - 2.
(8)
Fluorescence recovery after photobleaching FRAP
FRAP
(ROI)
( )
1
GM3 IR caveolin-1 FRAP
GM3 GM3
IR GM3
IR/ caveolin-1 (4) 2
- 6 -
Y
2 2
FITC
20 ( 3A)
Rhodamine-C18
2.0 µl/min 5.0 µl/min
( 3B) EGFP CHO-K1
( 4A) (IR-EGFP)
β- MβCD
FRAP MβCD
FRAP ( 4B) IR-EGFP ( 4C)
”
- 7 - 3.
2 GM3
GM3
DRM
GM3 TOF-MS DRM Triton X-100
MS
1,2- DCE
( 99.99%
) MS
(6)
TNF-α
3T3-L1 DRM
DCE Triton X-100
DRM LC-MS
MS
Svennerholm
LC-MS 100 pM 96 TNF-α
GM3 2
5
3T3-L1 TNF-α GM3 2
C57BL/6J
KK KKAy
C16 C18 (9)
ob/ob C16 SM
C24 SM (10)
GM3 LC-MS
2
4.
- 8 -
LC-MS
5.
1. Kabayama K, Ito N, Honke K, Igarashi Y, & Inokuchi J (2001) Suppression of integrin expression and tumorigenicity by sulfation of lactosylceramide in 3LL Lewis lung carcinoma cells. J Biol Chem 276(29):26777-26783.
2. Tagami S, et al. (2002) Ganglioside GM3 participates in the pathological conditions of insulin resistance. J Biol Chem 277(5):3085-3092.
3. Kabayama K, et al. (2005) TNF-α-induced insulin resistance in adipocytes as a membrane microdomain disorder: involvement of ganglioside GM3. Glycobiology 15(1):21-29.
4. Kabayama K, et al. (2007) Dissociation of the insulin receptor and caveolin-1 complex by
ganglioside GM3 in the state of insulin resistance. Proc Natl Acad Sci USA 104(34):13678-13683.
5. Sekimoto J, Kabayama K, Gohara K, & Inokuchi J (2012) Dissociation of the insulin receptor from caveolae during TNFalpha-induced insulin resistance and its recovery by D-PDMP. FEBS Lett 586(2):191-195.
6. Suzuki Y & Kabayama K (2012) Convenient and rapid removal of detergent from glycolipids in detergent-resistant membrane microdomains. J Lipid Res 53(3):599-608.
7. Kojima H, Suzuki Y, Ito M, & Kabayama K (2015) Structural characterization of neutral glycosphingolipids from 3T3-L1 Adipocytes. Lipids 50(9):913-917.
8. Kabayama K (2012) Modulation of growth factor receptors in membrane microdomains. Yakugaku Zasshi 132(4):417-423.
9. Tanabe A, et al. (2009) Obesity causes a shift in metabolic flow of gangliosides in adipose tissues.
Biochem Biophys Res Commun 379(2):547-552.
10. Samad F, Hester KD, Yang G, Hannun YA, & Bielawski J (2006) Altered adipose and plasma sphingolipid metabolism in obesity: a potential mechanism for cardiovascular and metabolic risk.
Diabetes 55(9):2579-2587.
- 9 - [email protected]
DNA
DNA A T G C
DNA DNA RNA
DNA Origami
Romesberg 2014
[1] D-ribose
[2]
D-(deoxy)ribose D-threoninol D-threoninol
D-ribose
[3]
NMR [3b]
- 10 -
[4]
Tyagi Molecular Beacon
MB
[5] MB
In Stem Molecular Beacon
(ISMB) MB DNA RNA
[4a]
PLL-g-Dex
ISMB DNA
DNA M DNA
- 11 -
ISMB 500
[4c] Cy3 ISMB [4b, 4e]
RNA [4e]
[6]
DNA p-
4A [6a]
DNA G-C
4B p-
[2+2]
340nm
4C [6c]
[7]
[8]
5A π
DNA
NMR
(A) (B)
(C) (B)
Z
- 12 -
5B
[8b]
5C [8a]
[8c, 8d]
[9]
D-threoninol
P Q
P-Q DNA DNA P-P Q-Q
(A) (B) (C)
- 13 - DNA
DNA RNA
- 14 -
N. Dai, J. M. Foster, I. R. Correa, F. E. Romesberg, Nature 2014, 509, 385-388.
[2] J. Štambaský, M. Hocek, P. Kočovský, Chem. Rev. 2009, 109, 6729-6764.
[3] a) H. Kashida, T. Fujii, H. Asanuma, Org. Biomol. Chem. 2008, 6, 2892-2899; b) T. Fujii, H. Kashida, H.
Asanuma, Chem. Eur. J. 2009, 15, 10092-10102.
[4] a) H. Kashida, T. Takatsu, T. Fujii, K. Sekiguchi, X. Liang, K. Niwa, T. Takase, Y. Yoshida, H. Asanuma, Angew. Chem. Int. Ed. 2009, 48, 7044-7047; b) Y. Hara, T. Fujii, H. Kashida, K. Sekiguchi, X. Liang, K. Niwa, T. Takase, Y. Yoshida, H. Asanuma, Angew. Chem. Int. Ed. 2010, 49, 5502-5506; c) H. Asanuma, T. Osawa, H. Kashida, T. Fujii, X. Liang, K. Niwa, Y. Yoshida, N. Shimada, A. Maruyama, Chem. Commun. 2012, 48, 1760-1762; d) T. Fujii, Y. Hara, T. Osawa, H. Kashida, X. Liang, Y. Yoshida, H. Asanuma, Chem. Eur. J.
2012, 18, 10865-10872; e) H. Kashida, T. Osawa, K. Morimoto, Y. Kamiya, H. Asanuma, Bioorg. Med. Chem.
2015, 23, 1758-1762.
[5] S. Tyagi, F. R. Kramer, Nat. Biotechnol. 1996, 14, 303-308.
[6] a) H. Kashida, H. Ito, T. Fujii, T. Hayashi, H. Asanuma, J. Am. Chem. Soc. 2009, 131, 9928-9930; b) H.
Kashida, T. Hayashi, T. Fujii, H. Asanuma, Chem. Eur. J. 2011, 17, 2614-2622; c) H. Kashida, T. Doi, T.
Sakakibara, T. Hayashi, H. Asanuma, J. Am. Chem. Soc. 2013, 135, 7960-7966.
[7] a) T. Doi, H. Kashida, H. Asanuma, Org. Biomol. Chem. 2015, 13, 4430-4437; b) T. Doi, H. Kawai, K.
Murayama, H. Kashida, H. Asanuma, Chem. Eur. J., in press.
[8] a) H. Kashida, K. Sekiguchi, H. Asanuma, Chem. Eur. J. 2010, 16, 11554-11557; b) H. Kashida, K. Sekiguchi, N. Higashiyama, T. Kato, H. Asanuma, Org. Biomol. Chem. 2011, 9, 8313-8320; c) H. Kashida, H. Asanuma, Phys. Chem. Chem. Phys. 2012, 14, 7196-7204; d) H. Kashida, N. Higashiyama, T. Kato, H. Asanuma, Bioorg.
Med. Chem. 2013, 21, 6191-6197.
[9] T. Doi, T. Sakakibara, H. Kashida, Y. Araki, T. Wada, H. Asanuma, Chem. Eur. J. 2015, 21, 15974-15980.
- 15 -
ImPACT [email protected]
2010
2016 1
ImPACT
nm µm
DNA MOF bottom-up
D MEMS top-down
4
Programmable motion of DNA origami mechanisms
A. E. Marras, L. Zhou, H.-J. Su, and C. E. Castro, Proc. Natl. Acad. Sci. USA, 2015, 112, 713–718.
DNA 100 nm
DNA
2 3
DNA DNA
1a
1b TEM 11-147 79.7°
29.2 nm
2 -
1c TEM
1 DNA TEM (a) (b)
(c) - (d, e) Bennett
- 16 -
Programmed switching of single polymer conformation on DNA origami
A. Krissanaprasit,M. Madsen,J. B. Knudsen, D. Gudnason,W. Surareungchai,V. Birkedal,and K. V.
Gothelf, ACS Nano 2016, 10, 2243−2250.
DNA DNA DNA
2
APPV 9
DNA-APPV 2a
DNA
DNA DNA
2b
AFM DNA
2b 2b
DNA
RgDNA 2c
LgDNA;
2d DNA DNA R DNA LrDNA
2e
2 DNA FRET
6
3D printed microtransporters: Compound micromachines for spatiotemporally controlled delivery of therapeutic agents
T.-Y. Huang, M. S. Sakar, A. Mao, A. J. Petruska, F. Qiu, X.-B. Chen, S. Kennedy, D. Mooney, and B. J.
2 (a) DNA (b)
DNA (c) (d)
(e)
- 17 - Nelson; Adv. Mater. 2015, 27, 6644–6650.
µm
direct laser writing
3a
3b
3c 3d
Cellular cargo delivery: Toward assisted fertilization by sperm-carrying micromotors
M. Medina-Sańchez, L. Schwarz, A. K.Meyer,F. Hebenstreit,and O. G. Schmidt; Nano Lett. 2016, 16, 555−561.
4a
Adv. Mater.
2015
70 µm/s
HOS 4bi
4ci 4cii
4ciii
3 (a) (b) (c)
( ) ( ) (d)
4 (a) (b) (i) (ii)
(c) (i) (ii)
(iii)
- 18 -
- 19 - Gao Jingchi
2
Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow
Ma, H.; Tu, L. C.; Naseri, A.; Huisman, M.; Zhang. S.; Grunwald, D.; Pederson, T. Nat Biotechnol. 2016, 34, 528−530.
CRISPR/Cas9
CRISPR/Cas9
Cas9 dCas9
RNA Single-guide RNA, sgRNA
dCas9
sgRNA dCas9
CRISPR/Cas9
CRISPRainbow
dCas9 sgRNA dCas9
sgRNA
1. CRISPRainbow
- 20 -
2. CRISPRainbow
MCP BFP PP7 PCP GFP boxB
N22 RFP 1 1 sgRNA
1 sgRNA 7
CRISPRainbow 15
CRISPRainbow
sgRNA
dCas9 sgRNA U2OS
2
CRISPRainbow dCas9
Visualization of protein−specific glycosylation inside living cells
Doll, F.; Buntz, A.; Späte A. K.; Schart, V. F.; Timper, A.; Schrimpf, W.; Hauck, C. R.; Zumbusch, A.;
Wittmann, V. Angew. Chem. Int. Ed. 2016, 55, 2262−2266.
Glycosylation
N O-GlcNAc 3
Protein of interest, POI EGFP
Ac4GlcNCyoc
Ac4GlcNCyoc Inverse-electron-demand Diels-Alder
reaction, DAinv EGFP
FRET EGFP EGFP
- 21 -
4.
3.
FRET
Fluorescence lifetime imaging microscopy, FLIM
FLIM
FRET
EGFP Ac4GlcNCyoc
Ac4GlcNAc
FRET
EGFP
Foxo1 p53 Akt1 4
O-GlcNAc
EGFP HEK293T
Ac4GlcNCyoc
TAMRA-Tz FLIM-FRET
FRET EGFP
Ac4GlcNAc FRET
Akt1
Akt1 O-GlcNAc
- 22 - [email protected]
2015 1 2016 2
2016 3
UC Christopher J. Chang Chris
Jefferson Chan Jeff
Jeff
iCeMS
Jeff
University of Illinois at Urbana–Champaign, UIUC
−20
UIUC Prof. Lu Prof. Moore
- 23 - NMR
UIUC Jeff
Jeff
Jeff
Jeff Jeff
Jeff
- 24 - Jeff
Genetically-encoded
sensors Jeff
“proposal competition”
iCeMS
Jeff
Jeff
Lab Olympic
8 2014 SciFinder Future Leaders in Chemistry
https://www.cas.org/products/scifinder/futureleaders-alumni CAS Chemical Abstract Service
SciFinder CAS
Mt. Hood
- 25 -
2014 “ ” Mt. Hood
Jeff
Jeff
- 26 -
第 19 回 生命化学研究会 〜生命化学・温故知新〜
https://res.tagen.tohoku.ac.jp/FBC/
2016 8 1 13:30 ~ 2 12:00
(https://www.kgh.ne.jp/10/access/)
1. JR 60
2. 90
)
JAL 291 0800- 0935, ANA 3811 0720- 0900
3 0845- 1038
545- 731( ) 0804- 0943- 1043
541 0943- 1020
8,000 18,360
E-mail 6 20
tsumu @ b.s.osakafu-u.ac.jp @
) )
40
6 20
A4 tsumu @
b.s.osakafu-u.ac.jp @ ) )
(https://res.tagen.tohoku.ac.jp/FBC/)
) ___ __
(
599-8531 1-1
TEL 072-254-9834
- 27 -
第 10 回 バイオ関連化学シンポジウム
（第 31 回 生体機能関連化学シンポジウム、第 19 回 バイオテクノロジー部会シンポジウム）
http://jointsympo.csj.jp/
-
-
9 7 9
JR 6 29 7 15
7 22 )
1 2 3
4 5 6 7
◎ 1 2
◎ 15 5
* 1 1 2
WEB http://jointsympo.csj.jp/
7,000 3,000
9,000 4,000
7 22 9,000 5,000
11,000 6,000
9 8 ANA
8,000
WEB http://jointsympo.csj.jp/
/
923-1292 1-1
10
/FAX (0761)51-1650 E-mail: [email protected] http://jointsympo.csj.jp/
- 28 -
2016 9 27 29
5 860-0047 1-14-1
1. Chemistry of Nucleosides, Nucleotides, and Their Analogues 2. Medicinal Chemistry of Nucleosides and Oligonucleotides 3. DNA/RNA Chemistry and Biochemistry
4. DNA/RNA Structure and Recognition 5. Ribozymes, siRNAs, and miRNAs 6. DNA/RNA Materials and Diagnostics
7. Drug Delivery Systems and Nanotechnology of Oligonucleotides
2016 4 11 6 17
2016 4 11 7 8
2016 4 11 8 12
30,000 35,000
10,000 15,000
http://isnac2016.org/
E-mail: [email protected]
Tel: 096-342-3873, Fax: 096-342-3679
- 29 -
第 26 回 バイオ・高分子シンポジウム
http://www.spsj.or.jp/entry/7 28 ( ) 29 ( ) 28 ( )
9
)
)
(1)
(2)
(3)
(4)
(5)
(6)
1.
2.
3. 20 12 8
4
1)
7,560
5,400 3,240
2) 5,000 2,500
http://www.spsj.or.jp/entry/
