኱ࡁࡃపୗࡋ㸪ࡑࡢᚋ⦆ࡸ࠿࡟పୗࡍࡿഴྥࢆ♧ࡋࡓࠋTDO ⩌࡛ࡣ㸪ᐇ㦂ᮇ㛫ࢆ㏻ ࡌ࡚㡢㏿࡟᭷ពᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋTDR⩌࡟࠾࠸࡚ࡣ㸪TDሬᕸᚋ࡟㡢㏿ࡢୖ᪼ ࡀㄆࡵࡽࢀ㸪௨ᚋࡢ㡢㏿࡟㢧ⴭ࡞ኚ໬ࡣ࡞ࡃ㸪Control࡜᭷ពᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋ ࡇࡢࡼ࠺࡟㸪TDO ࠾ࡼࡧ TDR

ࣜࣜࣥ㓟࢝ࣝࢩ࣒࢘⣔▱ぬ㐣ᩄᢚไᮦࡢ

⬺⅊ᢚไ࡞ࡽࡧ࡟㇟∳⣽⟶ᑒ㙐ᛶ࡟㛵ࡍࡿ◊✲

᪥ᮏ኱Ꮫ኱Ꮫ㝔ṑᏛ◊✲⛉ṑᏛᑓᨷ 㐲⸨ ⫕

㸦ᣦᑟ㸸ᐑᓮ ┿⮳ ᩍᤵ㸧

ᴫ せ

㇟∳㉁▱ぬ㐣ᩄ⑕ࡣ㸪㇟∳㉁ࡢ㟢ฟ࡟కࡗ࡚㇟∳⣽⟶ࡀ㛤ཱྀࡋ㸪ࡑࡢෆ㒊ࡢ⤌⧊ᾮ ࡀ⛣ືࡍࡿࡇ࡜࡟ࡼࡗ࡚⚄⤒⤊ᮎࡀ่⃭ࡉࢀ㸪⑊③ࡀច㉳ࡉࢀࡿ⑌ᝈ࡛࠶ࡿࠋ㇟∳㉁

▱ぬ㐣ᩄ⑕࡟ᑐࡍࡿ἞⒪ἲࡣ࠸ࡃࡘ࠿ᣲࡆࡽࢀࡿࡀ㸪㛤ཱྀࡋࡓ㇟∳⣽⟶ࢆᑒ㙐ࡍࡿࡇ

࡜ࡀຠᯝⓗ࡛࠶ࡿ࡜⪃࠼ࡽࢀ࡚࠸ࡿࠋࡇࡢ㇟∳⣽⟶ࡢᑒ㙐ἲ࡜ࡋ࡚ࡣ㸪㇟∳㉁⾲㠃࡛

ࡢ⿕⭷ᙧᡂ࠶ࡿ࠸ࡣ⣽⟶ෆࢱࣥࣃࢡ㉁ᡂศࡢจᅛ࡞࡝ࡀ࠶ࡾ㸪ࡇࢀࡽࢆ┠ⓗ࡜ࡋࡓ▱

ぬ㐣ᩄᢚไᮦࡀ⮫ᗋᛂ⏝ࡉࢀ࡚࠸ࡿࠋࡇࡢ࠺ࡕ㸪㇟∳⣽⟶ࢆᑒ㙐ࡍࡿ᪉ἲ࡜ࡋ࡚ࡣ㸪

ࣞࢪࣥ⣔ᮦᩱࢆṑ㠃࡟ሬᕸࡍࡿࡶࡢ࡜㸪⣽⟶ෆ࡟࢝ࣝࢩ࣒࢘ሷ࡞࡝ࢆᯒฟࡉࡏࡿࡶࡢ

࡞࡝ࡀ࠶ࡿࠋ๓⪅ࡢ᪉ἲࡣ㸪ṑ㠃ࡢ☜ᐇ࡞஝⇱ࡸṑ㠃ࡢΎᤲ࡞࡝㸪㐺ษ࡞᥋╔ࢆ⋓ᚓ ࡍࡿࡓࡵ࡟ࡣཝᐦ࡞᧯సࢆᚲせ࡜ࡍࡿࠋ୍᪉㸪ᚋ⪅ࡢ᪉ἲࡣ㸪ᯒฟ≀ࡢᙧᡂ࡟᫬㛫ࢆ

せࡍࡿ࡜࡜ࡶ࡟㸪㓟ᛶ㣧㣗≀ࡢᦤྲྀ࡜࡜ࡶ࡟⁐ゎࡍࡿ࡜࠸࠺ḞⅬࢆ᭷ࡋ࡚࠸ࡿࠋࡑࡇ

࡛㸪⮫ᗋⓗ࡟⡆౽࡞᧯స࡛㸪☜ᐇ࡟㇟∳⣽⟶ࢆᑒ㙐ࡍࡿ▱ぬ㐣ᩄᢚไᮦࡀᮃࡲࢀ࡚࠸

ࡿࠋ

ࡑࡇ࡛㸪᪂ࡓ࡟㛤Ⓨࡉࢀࡓ⮬ᕫ◳໬ᛶࣜࣥ㓟࢝ࣝࢩ࣒࢘⣔▱ぬ㐣ᩄᢚไᮦࢆ⏝࠸㸪 ࡑࡢ⬺⅊ᢚไ࠾ࡼࡧ㇟∳⣽⟶ᑒ㙐ᛶ࡟ࡘ࠸࡚㸪㉸㡢Ἴ㏱㐣ἲ࡜࡜ࡶ࡟࣮ࣞࢨ࣮㉮ᰝ㢧 ᚤ㙾㸦LSM㸧ࢆ⏝࠸᳨࡚ウࡋࡓࠋࡉࡽ࡟㸪㉮ᰝᆺ㟁Ꮚ㢧ᚤ㙾㸦SEM㸧ほᐹࢆ⾜࠺࡜࡜

ࡶ࡟࢚ࢿࣝࢠ࣮ศᩓᆺX⥺ศᯒ㸦EDX㸧ࢆ⏝࠸࡚ඖ⣲ศᯒࢆ⾜࠸㸪⪃ᐹ㈨ᩱ࡜ࡋࡓࠋ  ᐃ࡟ࡣ㸪࢘ࢩୗ㢡๓ṑࡢ㇟∳㉁ࢆ4™4™1 mmࡢࣈࣟࢵࢡయ࡟ㄪᩚࡋ㸪⪏Ỉᛶࢩ

ࣜࢥ࣮ࣥ࢝ࣂ࢖ࢻ࣮࣌ࣃ࣮ࡢ #600࠿ࡽ #2000ࡲ࡛㡰ḟ◊☻ࡋ㸪30ศ㛫⢭〇Ỉ୰࡛㉸

㡢ἼὙίࢆࡋࡓࡶࡢࢆ⏝࠸ࡓࠋ▱ぬ㐣ᩄᢚไᮦ࡜ࡋ࡚ࡣ㸪ࢸ࢕࣮ࢫ࣓࢖ࢺࢹ࢕ࢭࣥࢩ

ࢱ࢖ࢨ࣮㸦TD㸪ࢡࣛࣞࣀࣜࢱࢣࢹࣥࢱࣝ㸧ࢆ⏝࠸ࡓࠋ

ヨ∦࡟ᑐࡍࡿ⬺⅊᮲௳࡜ࡋ࡚ࡣ㸪1᪥࡟ࡘࡁ2ᗘ㸪0.1 Mࡢங㓟⦆⾪ᾮ㸦pH 4.75㸧

࡟10ศ㛫ᾐₕࢆ⾜࠸㸪37 Υேᕤၚᾮ࡟ಖ⟶ࡋࡓࠋᐇ㦂ᮇ㛫ࢆ㏻ࡌ࡚ேᕤၚᾮ୰࡟ಖ

⟶ࡋࡓヨ∦ࢆ࣮࣋ࢫࣛ࢖ࣥ㸦Baseline㸧㸪TD ࢆሬᕸࡍࡿࡇ࡜࡞ࡃ⬺⅊᮲௳࡛ಖ⟶ࡋࡓ ヨ∦ࢆ De ⩌㸪TD ࢆሬᕸࡋ࡚㸪⬺⅊᮲௳࡛ಖ⟶ࡋࡓヨ∦ࢆ TDO ⩌㸪TD ࡢሬᕸࢆ 7

᪥ẖ࡟⾜࠸㸪⬺⅊᮲௳࡛ಖ⟶ࡋࡓヨ∦ࢆTDR⩌㸪TDࢆሬᕸࡋ㸪ᐇ㦂ᮇ㛫ࢆ㏻ࡌ࡚ே

ᕤၚᾮ୰࡟ಖ⟶ࡋࡓヨ∦ࢆࢥࣥࢺ࣮ࣟࣝ㸦Control㸧࡜ࡋࡓࠋ

㉸㡢Ἴ㏦ཷಙ⿦⨨࡜ࡋ࡚ࡣ㸪ࣃࣝࢧ࣮ࣞࢩ࣮ࣂ࣮㸦Model 5900PR㸪ࣃࢼ࣓ࢺࣜࢡࢫ㸧㸪

⦪Ἴ⏝ࢺࣛࣥࢫࢹ࣮ࣗࢧ࣮㸦V112㸪ࣃࢼ࣓ࢺࣜࢡࢫ㸧࠾ࡼࡧ࢜ࢩࣟࢫࢥ࣮ࣉ㸦Wave Runner LT584㸪ࣞࢡࣟ࢖㸧࠿ࡽᵓᡂࡉࢀࡿࢩࢫࢸ࣒ࢆ⏝࠸ࡓࠋ㉸㡢Ἴఏ᧛᫬㛫ࡢ ᐃ ࡣ㸪㉸㡢ἼὙί┤ᚋ㸦0᪥㸧㸪ᐇ㦂㛤ጞ7㸪14㸪21࠾ࡼࡧ28᪥ᚋ࡟タᐃࡋࡓࠋ

 ᐃࡣ㸪ヨ∦ࢆࢧࣥࣉࣝࢫࢸ࣮ࢪ࡟㟼⨨ࡋ࡚ࢺࣛࣥࢫࢹ࣮ࣗࢧ࣮ࢆᆶ┤࡟᥋ゐࡉࡏ㸪 㡢㏿ࡢఏ᧛᫬㛫࡜ヨ∦ࡢཌࡳ࡜࠿ࡽྛヨ∦ࡢ⦪Ἴ㡢㏿ࢆồࡵࡓࠋࡲࡓ㸪ヨ∦ࡢ⾲㠃ᛶ

≧ࢆ㸪LSM㸦VK-8700㸪࣮࢚࢟ࣥࢫ㸧ࢆ⏝࠸࡚ほᐹࡋࡓࠋSEMほᐹ࠾ࡼࡧඖ⣲ศᯒ࡟

ࡣ㸪㉸㡢Ἴ ᐃ⏝ヨ∦࡜ྠᵝࡢฎ⌮ࢆ⾜ࡗࡓヨ∦ࢆtert-ࣈࢱࣀ࣮ࣝ⃰ᗘୖ᪼⣔ิࢆ⏝

࠸࡚⬺Ỉࡋ㸪⮫⏺Ⅼ஝⇱㸦Model ID-3㸪࢚ࣜ࢜ࢽࢡࢫ㸧ࢆ⾜ࡗࡓࠋḟ࠸࡛㸪࢖࢜ࣥࢥ

࣮ࢱ࣮㸦Quick Coater Type SC-201㸪ࢧ࣮ࣥࣘ㟁Ꮚ㸧࡛㔠⵨╔ࢆ᪋ࡋ㸪SEM㸦ERA-8800FE㸪

࢚ࣜ࢜ࢽࢡࢫ㸧ࢆ⏝࠸࡚ຍ㏿㟁ᅽ10kVࡢ᮲௳࡛ほᐹࡍࡿ࡜࡜ࡶ࡟㸪EDX࡟ࡼࡿඖ⣲

⤌ᡂศᯒࢆ⾜ࡗࡓࠋ

ࡑࡢ⤖ᯝ㸪㡢㏿ࡢኚ໬ࡣྛ᮲௳࡟ࡼࡗ࡚␗࡞ࡾ㸪Baseline ࡟࠾ࡅࡿ㡢㏿ࡣᐇ㦂ᮇ㛫

ࢆ㏻ࡌ࡚ኚ໬ࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࡶࡢࡢ㸪De ⩌࡟࠾ࡅࡿ㡢㏿ࡣ㸪ᐇ㦂㛤ጞ 7 ᪥┠࡛

኱ࡁࡃపୗࡋ㸪ࡑࡢᚋ⦆ࡸ࠿࡟పୗࡍࡿഴྥࢆ♧ࡋࡓࠋTDO ⩌࡛ࡣ㸪ᐇ㦂ᮇ㛫ࢆ㏻

ࡌ࡚㡢㏿࡟᭷ពᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋTDR⩌࡟࠾࠸࡚ࡣ㸪TDሬᕸᚋ࡟㡢㏿ࡢୖ᪼

ࡀㄆࡵࡽࢀ㸪௨ᚋࡢ㡢㏿࡟㢧ⴭ࡞ኚ໬ࡣ࡞ࡃ㸪Control࡜᭷ពᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋ ࡇࡢࡼ࠺࡟㸪TDO ࠾ࡼࡧ TDR⩌࡟࠾࠸࡚ De⩌࡜ẚ㍑ࡋ࡚㧗࠸㡢㏿ࡀㄆࡵࡽࢀࡓࡢ ࡣ㸪TD ࡀ㇟∳⣽⟶ෆ࡟౵ධࡋ࡚㸪ࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࢆᙧᡂࡍࡿ࡜࡜ࡶ࡟㸪㇟

∳⣽⟶ෆ࡟ࡶ⤖ᬗᵓ㐀≀ࡀ௜╔ࡋࡓࡓࡵ࡜⪃࠼ࡽࢀࡓࠋࡲࡓ㸪TDO⩌࡜ࡣ␗࡞ࡾTDR

⩌࡛ࡣ㸪TD ࢆ཯᚟ሬᕸࡍࡿࡓࡵ࡟㸪ṑ㉁࡟ᑐࡍࡿ㓟ࡢᙳ㡪ࡀῶᙅࡉࢀࡓࡶࡢ࡜⪃࠼

ࡽࢀࡓࠋ

LSM࠾ࡼࡧSEMほᐹ࠿ࡽࡣ㸪㉸㡢ἼὙίᚋࡢヨ∦࡛ࡣ㸪ࢫ࣑࢔࣮ࣉࣛࢢࡣ᏶඲࡟

㝖ཤࡉࢀ㸪㇟∳⣽⟶ࡀ㛤ཱྀࡋࡓീࡀほᐹࡉࢀࡓࠋTD ሬᕸ┤ᚋ࡛ࡣ㸪⣽⟶ࡣ᏶඲࡟ᑒ 㙐ࡉࢀ㸪㇟∳㉁⾲㠃࡟⢏≧ሁ✚≀ࡀㄆࡵࡽࢀࡓࠋControl ࡟࠾࠸࡚ࡣ㸪㇟∳㉁⾲㠃࡟

⢏≧ሁ✚≀ࡀㄆࡵࡽࢀ㸪ᐇ㦂ᮇ㛫ࢆ㏻ࡌ࡚ࡑࡢ⾲㠃ᛶ≧࡟ኚ໬ࡣ࡞࠿ࡗࡓࠋTDO ⩌

࡛ࡣ㸪୍㒊࡟㇟∳⣽⟶ࡢ㛤ཱྀࡀㄆࡵࡽࢀࡓࡶࡢࡢ㸪TDR⩌࡛ࡣ㸪㇟∳⣽⟶ࡀ㛢ሰࡉࢀ

ࡿ࡜࡜ࡶ࡟ TD ሬᕸ㠃࡟ᯒฟ≀ࡀㄆࡵࡽࢀࡓࠋTD ࢆ⧞ࡾ㏉ࡋሬᕸࡍࡿࡇ࡜࡟ࡼࡗ࡚

㇟∳㉁⾲ᒙ࡟ᙧᡂࡉࢀࡓ⤖ᬗᵓ㐀≀ࡣ⦓ᐦ࡞ᵓ㐀࡜࡞ࡗ࡚࠾ࡾ㸪⤖ᬗᛶࡀྥୖࡋࡓྍ

⬟ᛶࡀ♧၀ࡉࢀࡓࠋ

EDX ࡟ࡼࡿඖ⣲ศᯒ࠿ࡽ㸪Baseline ࡛ࡣ㸪࢝ࣝࢩ࣒࢘࡜ࣜࣥ࡟ࣆ࣮ࢡࡀㄆࡵࡽࢀ㸪 Ca/Pẚࡣ1.84࡛࠶ࡗࡓࠋTDR⩌࡟࠾࠸࡚ࡶ࢝ࣝࢩ࣒࢘࡜ࣜࣥ࡟ࣆ࣮ࢡࡀㄆࡵࡽࢀ㸪 Ca/Pẚࡣ1.86࡛࠶ࡗࡓࠋࡇࡢࡼ࠺࡟Baseline࡜TDR⩌࡜࡛ᕪࡀ࡞࠿ࡗࡓ࡜ࡇࢁ࠿ࡽ㸪

ṑ㉁࡟㏆ఝࡋࡓࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࡀᙧᡂࡉࢀ࡚࠸ࡿࡇ࡜ࡀ♧၀ࡉࢀࡓࠋ ௨ୖࡢࡼ࠺࡟㸪ᮏ◊✲ࡢ⤖ᯝ࠿ࡽ㸪ࣜࣥ㓟࢝ࣝࢩ࣒࢘⣔▱ぬ㐣ᩄᢚไᮦ࡛࠶ࡿ TD ࡣ㸪㇟∳㉁ࡢ⬺⅊ᢚไ࠾ࡼࡧ㇟∳⣽⟶ᑒ㙐⬟ࢆ᭷ࡍࡿࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡗࡓࠋࡲࡓ㸪

⏕యぶ࿴ᛶ࡟ඃࢀࡿ࡜⪃࠼ࡽࢀ㸪⮫ᗋ࡟࠾ࡅࡿ᭷ຠᛶࡀᮇᚅࡉࢀࡿࠋ

࡞࠾㸪ᮏㄽᩥࡣ㸪ཎⴭㄽᩥ Endo H, Kawamoto R, Takahashi F, Takenaka H, Yoshida F, Nojiri K, Takamizawa T, Miyazaki M. Evaluation of a calcium phosphate desensitizer using an ultrasonic device. Dent Mater J 2013; 32: 456-461 ࢆᇶᖿㄽᩥ࡜ࡋ㸪ࡇࢀ࡟ඖ⣲⤌ᡂศ ᯒࡢᐇ㦂ࢹ࣮ࢱࢆຍ࠼ࡿࡇ࡜࡟ࡼࡗ࡚⥲ᣓࡋࡓࡶࡢ࡛࠶ࡿࠋ

⥴ ゝ

㇟∳㉁▱ぬ㐣ᩄ⑕ࡣ㸪㇟∳㉁ࡢ㟢ฟ࡟కࡗ࡚㇟∳⣽⟶ࡀ㛤ཱྀࡋ㸪ࡑࡢෆ㒊ࡢ⤌⧊ᾮ ࡀ⛣ືࡍࡿࡇ࡜࡟ࡼࡗ࡚⚄⤒⤊ᮎࡀ่⃭ࡉࢀ࡚⑊③ࡀច㉳ࡉࢀࡿ⑌ᝈ࡛࠶ࡿ ^1-3) ࠋ㇟

∳㉁▱ぬ㐣ᩄ⑕࡟ᑐࡍࡿ἞⒪ἲࡣ࠸ࡃࡘ࠿ᣲࡆࡽࢀࡿࡀ⁴⁾ 㸪㛤ཱྀࡋࡓ㇟∳⣽⟶ࢆᑒ㙐 ࡍࡿࡇ࡜ࡀຠᯝⓗ࡛࠶ࡿ࡜⪃࠼ࡽࢀ࡚࠸ࡿ⁵⁾ ࠋࡇࡢ㇟∳⣽⟶ࡢᑒ㙐ἲ࡜ࡋ࡚ࡣ㸪㇟∳

㉁⾲㠃࡛ࡢ⿕⭷ᙧᡂ࠶ࡿ࠸ࡣ⣽⟶ෆࢱࣥࣃࢡ㉁ᡂศࡢจᅛ࡞࡝ࡀ࠶ࡾ㸪ࡇࢀࡽࢆ┠ⓗ

࡜ࡋࡓ▱ぬ㐣ᩄᢚไᮦࡀ⮫ᗋᛂ⏝ࡉࢀ࡚࠸ࡿ⁶⁾ ࠋࡉࡽ࡟㸪㇟∳⣽⟶ࢆᑒ㙐ࡍࡿ᪉ἲ࡜

ࡋ࡚ࡣ㸪ࣞࢪࣥ⣔ᮦᩱࢆṑ㠃࡟ሬᕸࡍࡿࡶࡢ࡜㸪⣽⟶ෆ࡟࢝ࣝࢩ࣒࢘ሷ࡞࡝ࢆᯒฟࡉ ࡏࡿࡶࡢ࡞࡝ࡀ࠶ࡿࠋ๓⪅ࡢ᪉ἲࡣ㸪ṑ㠃ࡢ☜ᐇ࡞஝⇱ࡸṑ㠃ࡢΎᤲ࡞࡝㸪㐺ษ࡞᥋

╔ࢆ⋓ᚓࡍࡿࡓࡵ࡟ࡣཝᐦ࡞᧯సࢆᚲせ࡜ࡍࡿࠋ୍᪉㸪ᚋ⪅ࡢ᪉ἲࡣ㸪ᯒฟ≀ࡢᙧᡂ

࡟᫬㛫ࢆせࡍࡿ࡜࡜ࡶ࡟㸪㓟ᛶ㣧㣗≀ࡢᦤྲྀ࡜࡜ࡶ࡟⁐ゎࡍࡿ࡜࠸࠺ḞⅬࢆ᭷ࡋ࡚࠸

ࡓࠋࡑࡇ࡛㸪⮫ᗋⓗ࡟⡆౽࡞᧯స࡛㸪☜ᐇ࡟㇟∳⣽⟶ࢆᑒ㙐ࡍࡿ▱ぬ㐣ᩄᢚไᮦࡀᮃ

ࡲࢀ࡚࠸ࡿࡢࡀ⌧≧࡛࠶ࡿ⁷⁾ࠋ

ࣜࣥ㓟Ỉ⁐ᾮ୰࡛㸪ࣜࣥ㓟ᅄ࢝ࣝࢩ࣒࢘㸦௨ᚋ㸪TTCP㸧࡜↓Ỉࣜࣥ㓟Ỉ⣲࢝ࣝࢩ

࣒࢘㸦௨ᚋ㸪DCPA㸧࡜ࡀ1㸸1ࡢࣔࣝẚ࡛Ꮡᅾࡍࡿ࡜㸪Ỉࡀ௓ᅾࡍࡿࡇ࡜࡟ࡼࡗ࡚ࣁ

࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࡀᙧᡂࡉࢀࡿࡇ࡜ࡀሗ࿌ࡉࢀ࡚࠸ࡿ⁸⁾ ࠋࡇࡢ཯ᛂࢆᛂ⏝ࡋࡓࣜ

ࣥ㓟࢝ࣝࢩ࣒࢘ࢭ࣓ࣥࢺ࡟ࡘ࠸࡚ࡣ㸪ṑࡢ▼⅊໬⏝〇๣ ⁹⁾ 㸪᰿⟶඘ሸᮦ¹⁰⁾ ࠶ࡿ࠸ࡣ 㦵⿵ሸᮦ ¹¹⁾ ࡞࡝ࡢ᳨ウࡀ⾜ࢃࢀ࡚࠾ࡾ㸪▱ぬ㐣ᩄᢚไ࡬ࡢᛂ⏝࡟ࡘ࠸࡚ࡶ᳨ウࡉࢀ

࡚ࡁࡓ ¹²⁾ ࠋࡋ࠿ࡋ㸪ࣜࣥ㓟࢝ࣝࢩ࣒࢘ࢭ࣓ࣥࢺࡢḞⅬ࡜ࡋ࡚㸪TTCP ࡜ DCPA ࡜ࡢ

཯ᛂ࡟ 30 ศ௨ୖࡢ᫬㛫ࢆせࡍࡿࡇ࡜ࡀᣲࡆࡽࢀ㸪ࡇࢀࡀ⮫ᗋᛂ⏝࡟ࡘ࡞ࡀࡽ࡞࠿ࡗ

ࡓせᅉࡢࡦ࡜ࡘ࡜ࡉࢀ࡚ࡁࡓ ¹³⁾ ࠋࡑࡇ࡛㸪TTCP ࡜ DCPA ࡢ⢊ᮎ≉ᛶ࡟ኚ᭦ࢆຍ࠼

ࡿ࡜࡜ࡶ࡟ᚤ㔞ῧຍ≀ࢆㄪᩚࡍࡿࡇ࡜࡟ࡼࡗ࡚㸪࣮࣌ࢫࢺᛶ≧࡜࡜ࡶ࡟◳໬᫬㛫ࢆ㐺 ṇ໬ࡋࡓ▱ぬ㐣ᩄᢚไᮦࡀᕷ㈍ࡉࢀࡓࠋ

ࡑࡇ࡛ⴭ⪅ࡣ㸪ࡇࡢ⮬ᕫ◳໬ᛶࣜࣥ㓟࢝ࣝࢩ࣒࢘⣔▱ぬ㐣ᩄᢚไᮦࢆ⏝࠸㸪ࡑࡢ⬺

⅊ᢚไ࠾ࡼࡧ㇟∳⣽⟶ᑒ㙐ᛶ࡟ࡘ࠸࡚㸪㉸㡢Ἴ㏱㐣ἲ ¹⁴⁾ ࡜࡜ࡶ࡟࣮ࣞࢨ࣮㉮ᰝ㢧ᚤ 㙾㸦LSM㸧ࢆ⏝࠸᳨࡚ウࡋࡓࠋࡉࡽ࡟㸪㉮ᰝᆺ㟁Ꮚ㢧ᚤ㙾㸦SEM㸧ほᐹࢆ⾜࠺࡜࡜ࡶ

࡟࢚ࢿࣝࢠ࣮ศᩓᆺX⥺ศᯒ㸦EDX㸧ࢆ⏝࠸࡚ඖ⣲ศᯒࢆ⾜࠸㸪⪃ᐹ㈨ᩱ࡜ࡋࡓࠋ

ᮦᩱ࠾ࡼࡧ᪉ἲ 1 . ヨ∦ࡢ〇స

ᐇ㦂࡟ࡣ࢘ࢩୗ㢡๓ṑࢆ౑⏝ࡋ㸪ᐇ㦂࡟㝿ࡋ࡚◳⤌⧊⢭ᐦษ᩿ᶵ㸦Ecomet 4000㸪

Buehler㸧ࢆ⏝࠸࡚ࡑࡢ၁ഃ㇟∳㉁ࢆ⣙ 1mmࡢཌࡉ࡟ษ᩿ࡋࡓࠋḟ࠸࡛㸪ࢫ࣮ࣃ࣮ࣇ

࢓࢖ࣥࢲ࢖ࣖࣔࣥࢻ࣏࢖ࣥࢺ㸦ISO #021㸪ᯇ㢼㸧ࢆ⏝࠸࡚ࣈࣟࢵࢡయ㸦4™4™1 mm㸧

࡟ㄪᩚࡋ㸪ྛヨ∦ࡢ⾲㠃ࢆ⪏Ỉᛶࢩࣜࢥ࣮ࣥ࢝ࣂ࢖ࢻ࣮࣌ࣃ࣮ࡢ # 600࠿ࡽ# 2000 ࡲ

࡛ὀỈୗ࡛㡰ḟ◊☻ࡋࡓࠋ᭱⤊ⓗ࡞ヨ∦ࡢཌࡳ࠾ࡼࡧ኱ࡁࡉࡣ㸪࣐࢖ࢡ࣓࣮ࣟࢱ࣮

㸦CPM15-25DM㸪࣑ࢶࢺࣚ㸧ࢆ⏝࠸࡚ィ ࡋࡓࠋࡇࢀࡽࡢヨ∦ࢆ㸪⢭〇Ỉ୰࡛ 30 ศ

㛫㉸㡢ἼὙίࡍࡿࡇ࡜࡟ࡼࡗ࡚ࢫ࣑࣮ࣖࢆ㝖ཤࡋ㸪㇟∳⣽⟶ࡀ㛤ཱྀࡋࡓヨ∦ࢆ〇సࡋ ࡓࠋ

2 . ▱ぬ㐣ᩄᢚไᮦ࠾ࡼࡧேᕤၚᾮ

▱ぬ㐣ᩄᢚไᮦ࡜ࡋ࡚㸪⮬ᕫ◳໬ᛶࣜࣥ㓟࢝ࣝࢩ࣒࢘⣔࡛࠶ࡿࢸ࢕࣮ࢫ࣓࢖ࢺࢹ

࢕ࢭࣥࢩࢱ࢖ࢨ࣮㸦TD㸪ࢡࣛࣞࣀࣜࢱࢣࢹࣥࢱࣝ㸧ࢆ⏝࠸ࡓࠋTDࡢሬᕸ࡟࠶ࡓࡗ࡚㸪

㇟∳㉁ヨ∦ࢆྲྀࡾฟࡋ㸪ࢁ⣬ࢆ⏝࠸࡚వ๫Ỉศࢆྲྀࡾ㝖࠸ࡓࠋḟ࠸࡛㸪ヨ∦ࢆࢩࣜࢥ

࣮ࣥᆺ࡟㟼⨨ࡋ࡚㸪TD ࡀሬᕸ㠃௨እ࡟᥋ゐࡋ࡞࠸ࡼ࠺࡟ࡋࡓࠋࡲࡓ㸪ேᕤၚᾮ࡜ࡋ

࡚ࡣ㸪14.4 mM NaCl㸪16.1 mM KCl㸪0.3 mM MgCl2࣭6H2O2㸪2.0 mM K2HPO4㸪1.0 mM CaCl2࣭2H2O࠾ࡼࡧsodium carboxymethyl cellulose 0.1 g/100 mlࢆຍ࠼࡚pH 7.0 ࡟ㄪᩚ

ࡋࡓࡶࡢ࡜ࡋࡓ¹⁵⁾ ࠋ 3 . ಖ⟶᮲௳

ヨ∦ࡢಖ⟶᮲௳ࡣ㸪௨ୗ࡟♧ࡍ5⩌࡜ࡋ㸪ಖ⟶ᮇ㛫ࡣ࠸ࡎࢀࡶ28᪥㛫࡜ࡋࡓࠋ࡞

࠾㸪⬺⅊᧯సࡣ㸪1᪥࡟2ᅇ㸪28᪥㛫0.1 M ங㓟⦆⾪ᾮ¹⁶⁾ 㸦pH 4.75㸪0.75 mM CaCl2࣭ 2H2O࠾ࡼࡧ0.45 mM KH2PO4㸧࡟10ศ㛫స⏝ࡉࡏ㸪37Υேᕤၚᾮ୰࡟ಖ⟶ࡋࡓࠋ

1㸧 ࣮࣋ࢫࣛ࢖ࣥ㸦Baseline㸧㸸ヨ∦ࢆᐇ㦂ᮇ㛫ࢆ㏻ࡌ࡚ேᕤၚᾮ୰࡟ಖ⟶ࡋࡓࠋ 2㸧 De⩌㸸ヨ∦࡟TDࢆሬᕸࡍࡿࡇ࡜࡞ࡃ⬺⅊᮲௳࡛ಖ⟶ࡋࡓࠋ

3㸧 TDO⩌㸸ヨ∦࡟TDࢆ࣐࢖ࢡࣟࣈࣛࢩࢆ⏝࠸࡚30⛊㛫ሬᕸࡋ㸪ḟ࠸࡛⢭〇Ỉ

࡛ỈὙࡋࡓᚋ㸪⬺⅊᮲௳࡛ಖ⟶ࡋࡓࠋ

4㸧 TDR⩌㸸TDࡢሬᕸࢆ7᪥ẖ࡟⾜࠸㸪ᐇ㦂ᮇ㛫ࢆ㏻ࡌ࡚⬺⅊᮲௳࡛ಖ⟶ࡋࡓࠋ

5㸧 ࢥࣥࢺ࣮ࣟࣝ㸦Control㸧㸸ヨ∦࡟ TD ࢆሬᕸࡋ㸪ᐇ㦂ᮇ㛫ࢆ㏻ࡌ࡚ேᕤၚᾮ

୰࡟ಖ⟶ࡋࡓࠋ 4 . ㉸㡢Ἴ ᐃ

㉸㡢Ἴ㏦ཷಙ⿦⨨࡜ࡋ࡚ࡣ㸪ࣃࣝࢧ࣮ࣞࢩ࣮ࣂ࣮㸦Model 5900PR㸪ࣃࢼ࣓ࢺࣜࢡࢫ㸧㸪

⦪Ἴ⏝ࢺࣛࣥࢫࢹ࣮ࣗࢧ࣮㸦V112㸪ࣃࢼ࣓ࢺࣜࢡࢫ㸧࠾ࡼࡧ࢜ࢩࣟࢫࢥ࣮ࣉ㸦Wave

Runner LT584㸪ࣞࢡࣟ࢖㸧࠿ࡽᵓᡂࡉࢀࡿࢩࢫࢸ࣒¹⁷⁾ ࢆ⏝࠸ࡓ㸦Fig. 1㸧ࠋ㉸㡢Ἴఏ

᧛᫬㛫ࡢ ᐃࡣ㸪㉸㡢ἼὙί┤ᚋ㸦0᪥㸧㸪ᐇ㦂㛤ጞ7㸪14㸪21࠾ࡼࡧ28᪥ᚋ࡟タᐃ ࡋࡓࠋ࡞࠾TDR⩌ࡢ7᪥௨㝆࡟࠾࠸࡚ࡣ㸪TDሬᕸᚋ࡟ ᐃࢆ⾜ࡗࡓࠋ

 ᐃࡣ㸪ヨ∦ࢆࢧࣥࣉࣝࢫࢸ࣮ࢪ࡟㟼⨨ࡋ࡚ࢺࣛࣥࢫࢹ࣮ࣗࢧ࣮ࢆᆶ┤࡟᥋ゐࡉࡏ㸪

㉸㡢Ἴ㏱㐣ἲ࡟ࡼࡗ࡚⾜࠸㸪㡢㏿ࡢఏ᧛᫬㛫࡜ヨ∦ࡢཌࡳ࡜࠿ࡽྛヨ∦ࡢ⦪Ἴ㡢㏿ࢆ

ồࡵࡓࠋ࡞࠾㸪 ᐃࡣᐊ  23s1Υ㸪┦ᑐ‵ᗘ 50s5%ࡢᜏ ᜏ‵ᐊ࡛⾜࠸㸪ヨ∦ᩘ

ࡣྛ᮲௳࡟ࡘࡁ6ಶ࡜ࡋࡓࠋ 5 . ⤫ィศᯒ

ྛ᮲௳࡟࠾࠸࡚ᚓࡽࢀࡓ ᐃ್࡟ࡘ࠸࡚ࡣ㸪ศᩓศᯒ࡞ࡽࡧ࡟Tukey HSD test

ࢆ⏝࠸࡚㸪᭷ពỈ‽5%ࡢ᮲௳࡛⤫ィᏛⓗ᳨ᐃࢆ⾜ࡗࡓࠋ 6 . LSMほᐹ

ヨ∦⾲㠃ࡢᙧែⓗ࡞ほᐹ࡟ࡣ㸪LSM㸦VK-8700㸪࣮࢚࢟ࣥࢫ㸧ࢆ౑⏝ࡋࡓࠋ

7 . SEMほᐹ࠾ࡼࡧඖ⣲ศᯒ

㉸㡢Ἴ ᐃ⏝ヨ∦࡜ྠᵝࡢฎ⌮ࢆ⾜ࡗࡓヨ∦ࢆ tert-ࣈࢱࣀ࣮ࣝ⃰ᗘୖ᪼⣔ิࢆ⏝

࠸࡚⬺Ỉࡋ㸪⮫⏺Ⅼ஝⇱㸦Model ID-3㸪࢚ࣜ࢜ࢽࢡࢫ㸧ࢆ⾜ࡗࡓࠋḟ࠸࡛㸪࢖࢜ࣥࢥ

࣮ࢱ࣮㸦Quick Coater Type SC-201㸪ࢧ࣮ࣥࣘ㟁Ꮚ㸧࡛㔠⵨╔ࢆ᪋ࡋ㸪SEM㸦ERA-8800FE㸪

࢚ࣜ࢜ࢽࢡࢫ㸧ࢆ⏝࠸࡚ຍ㏿㟁ᅽ10 kVࡢ᮲௳࡛ほᐹࡍࡿ࡜࡜ࡶ࡟㸪EDX࡟ࡼࡿඖ⣲

⤌ᡂศᯒࢆ⾜ࡗࡓࠋ

ᡂ ⦼

ಖ⟶᮲௳ࡀ㸪࢘ࢩ㇟∳㉁ࢆ㏱㐣ࡍࡿ㡢㏿ࡢኚ໬࡟ཬࡰࡍᙳ㡪ࡢᡂ⦼ࢆTable 1࠾ࡼ

ࡧFig. 2࡟♧ࡋࡓࠋ㡢㏿ࡢኚ໬ࡣྛ᮲௳࡟ࡼࡗ࡚␗࡞ࡾ㸪Baseline࡟࠾ࡅࡿ㡢㏿ࡣᐇ

㦂ᮇ㛫ࢆ㏻ࡋ࡚ኚ໬ࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࡶࡢࡢ㸪De ⩌࡟࠾ࡅࡿ㡢㏿ࡣ㸪ᐇ㦂㛤ጞ 7

᪥┠࡛኱ࡁࡃపୗࡋ㸪ࡑࡢᚋ⦆ࡸ࠿࡟పୗࡍࡿഴྥࢆ♧ࡋࡓࠋTDO ⩌࡟࠾࠸࡚ࡣ㸪 㡢㏿ࡀపୗࡍࡿഴྥࢆ♧ࡋࡓࡀ㸪ᐇ㦂ᮇ㛫ࢆ㏻ࡌ࡚᭷ពᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋTDR

⩌࡟࠾࠸࡚ࡣ㸪TDሬᕸᚋ࡟㡢㏿ࡢୖ᪼ࡀㄆࡵࡽࢀ㸪௨ᚋࡢ㡢㏿࡟ኚ໬ࡣ࡞ࡃ㸪Control

࡜᭷ពᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࠋ

LSM࠾ࡼࡧSEMീࡢ௦⾲౛ࢆFigs. 3,4࡟♧ࡋࡓࠋ㉸㡢ἼὙίᚋࡢヨ∦࡛ࡣ㸪ࢫ࣑

࢔࣮ࣉࣛࢢࡣ᏶඲࡟㝖ཤࡉࢀ㸪㇟∳⣽⟶ࡀ㛤ཱྀࡋࡓീࡀほᐹࡉࢀࡓࠋTD ሬᕸ┤ᚋ࡛

ࡣ㸪⣽⟶ࡣ᏶඲࡟ᑒ㙐ࡉࢀ㸪㇟∳㉁⾲㠃࡟⢏≧ሁ✚≀ࡀㄆࡵࡽࢀࡓࠋControl ࡟࠾࠸

࡚ࡣ㸪㇟∳㉁⾲㠃࡟⢏≧ሁ✚≀ࡀㄆࡵࡽࢀ㸪ᐇ㦂ᮇ㛫ࢆ㏻ࡌ࡚ࡑࡢ⾲㠃ᛶ≧࡟ኚ໬ࡣ

࡞࠿ࡗࡓࠋTDO ⩌࡛ࡣ㸪୍㒊࡟㇟∳⣽⟶ࡢ㛤ཱྀࡀㄆࡵࡽࢀࡓࡶࡢࡢ㸪TDR ⩌࡛ࡣ㸪

㇟∳⣽⟶ࡀ㛢ሰࡉࢀࡿ࡜࡜ࡶ࡟TDሬᕸ㠃࡟ᯒฟ≀ࡀㄆࡵࡽࢀࡓࠋ

EDX࡟ࡼࡿඖ⣲⤌ᡂศᯒࡢ⤖ᯝࢆFig. 5࡟♧ࡋࡓࠋBaseline࡛ࡣ㸪࢝ࣝࢩ࣒࢘࡜ࣜ

ࣥ࡟ࣆ࣮ࢡࡀㄆࡵࡽࢀ㸪Ca/Pẚࡣ1.84࡛࠶ࡗࡓࠋTDR⩌࡟࠾࠸࡚ࡶ࢝ࣝࢩ࣒࢘࡜ࣜ

ࣥ࡟ࣆ࣮ࢡࡀㄆࡵࡽࢀ㸪Ca/Pẚࡣ1.86࡛࠶ࡗࡓࠋ

⪃ ᐹ

ṑ㉁࡟⏕ࡌࡿ⬺⅊࡜෌▼⅊໬࡜࠸࠺ࣉࣟࢭࢫࡣ㸪࡜ࡃ࡟ࡑࡢึᮇẁ㝵࡟࠾࠸࡚ᙧែ

ⓗ࡞ኚ໬࡜ࡋ࡚ᢕᥱࡍࡿࡇ࡜ࡣᅔ㞴࡛࠶ࡿࠋࡑࡇ࡛㸪ࡇࢀࢆほᐹࡍࡿࡓࡵ࡟࣐࢖ࢡࣟ

ࣛࢪ࢜ࢢ࣒ࣛ ¹⁸⁾ 㸪ඹ↔Ⅼ࣮ࣞࢨ࣮㢧ᚤ㙾 ^19,20) ࠶ࡿ࠸ࡣ Quantitative Light-induced

Fluorescenceἲ^21,22) ࡞࡝ࡀ⏝࠸ࡽࢀ࡚࠸ࡿࡀ㸪⿦⨨ࡀ኱ᆺ࡛࠶ࡿ࡞࡝ࡢၥ㢟Ⅼࢆ᭷ࡋ

࡚࠸ࡿࠋࡑࡇ࡛ⴭ⪅ࡣ㸪㠀◚ቯⓗ࡟ṑ㉁ࡢ⬺⅊⛬ᗘࢆ⤒᫬ⓗ࡟ᢕᥱ࡛ࡁࡿ࡜࡜ࡶ࡟㸪

⿦⨨඲యࡀẚ㍑ⓗᑠᆺ࡞ࡇ࡜ࢆ≉ᚩ࡜ࡍࡿ㉸㡢Ἴ㏱㐣ἲ࡟╔┠ࡋࡓࠋࡇࡢ㉸㡢Ἴ㏱㐣 ἲࡣ㸪ᕤᴗ⏺࠶ࡿ࠸ࡣ་Ꮫ㡿ᇦ࡟ᗈࡃᛂ⏝ࡉࢀ࡚࠾ࡾ㸪ṑ⛉㡿ᇦ࡟࠾࠸࡚ࡶṑ㉁ࡸṑ

⛉࡟࠾ࡅࡿ㉸㡢Ἴ≉ᛶ࡞࡝࡟ࡘ࠸࡚◊✲ࡉࢀ࡚࠸ࡿ ^23-25) ࠋᮏ◊✲࡛ࡣ㸪ࡇࡢ⿦⨨ࢆ

⏝࠸࡚㸪TD ࡀ㇟∳㉁ࡢ⬺⅊ᢚไ࠾ࡼࡧ㇟∳⣽⟶ᑒ㙐ᛶ࡟ཬࡰࡍᙳ㡪࡟ࡘ࠸᳨࡚ウࡋ ࡓࠋ

ࡑࡢ⤖ᯝ㸪Baseline࡟࠾ࡅࡿ㇟∳㉁ヨ∦ࡢ㡢㏿ࡣ3742㹼3775 m/s ࡛࠶ࡗࡓࡶࡢࡢ㸪 De⩌࡛ࡣ28᪥㛫⤒㐣ࡋࡓ᫬Ⅼ࡛3462 m/s ࡜᭷ព࡟పୗࡋࡓࠋࡲࡓ㸪TDO⩌࡛ࡣ㸪 ᐇ㦂ᮇ㛫ࢆ㏻ࡌ࡚㡢㏿࡟᭷ពᕪࡣㄆࡵࡽࢀ࡞࠿ࡗࡓࡀ㸪TDR⩌࡛ࡣ㡢㏿ࡀ᭷ព࡟ୖ᪼

ࡋࡓࠋࡇࡢࡼ࠺࡟㸪TDO ࠾ࡼࡧTDR ⩌࡟࠾࠸࡚ De⩌࡜ẚ㍑ࡋ࡚㧗࠸㡢㏿ࡀㄆࡵࡽ

ࢀࡓࡢࡣ㸪TD ࡀ㇟∳⣽⟶ෆ࡟౵ධࡋ࡚㸪ࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࢆᙧᡂࡍࡿ࡜࡜ࡶ

࡟²⁶⁾ 㸪㇟∳⣽⟶ෆ࡟ࡶ⤖ᬗᵓ㐀≀ࡀ௜╔ࡋࡓࡓࡵ࡜⪃࠼ࡽࢀࡓࠋࡲࡓ㸪TDO⩌࡜ࡣ

␗࡞ࡾTDR⩌࡛ࡣ㸪TDࢆ཯᚟ሬᕸࡍࡿࡓࡵ࡟㸪ṑ㉁࡟ᑐࡍࡿ㓟ࡢᙳ㡪ࡀῶᙅࡉࢀࡓ

ࡶࡢ࡜⪃࠼ࡽࢀࡓࠋ

LSMほᐹ࡛ࡣ㸪㉸㡢ἼὙί┤ᚋ㸦0᪥㸧࡟࠾࠸࡚ࡣ㸪㇟∳㉁⾲ᒙ࡟ࡣࢩࣜࢥ࣮ࣥ࢝

ࣂ࢖ࢻ࣮࣌ࣃ࣮ࡢ๐≧⑞ࡀㄆࡵࡽࢀ㸪㇟∳⣽⟶ࡀ㛤ཱྀࡋ࡚࠸ࡿീࡀほᐹࡉࢀࡓࠋࡇࡢ 㠃࡟TDࢆሬᕸࡍࡿ࡜㸪ヨ∦⾲㠃ࡣᯒฟ≀࡛そࢃࢀࡓീࢆ♧ࡋࡓࠋControl࡟࠾࠸࡚ࡣ㸪

TD ሬᕸᚋࡢ㇟∳㉁⾲㠃ᛶ≧࡟ኚ໬ࡣㄆࡵࡽࢀࡎ㸪ࣜࣥ㓟࠾ࡼࡧ࢝ࣝࢩ࣒࢘࢖࢜ࣥࡀ 㐣㣬࿴࡞⎔ቃ࡛ࡣ㸪TD ሬᕸ࡟ࡼࡗ࡚⏕ࡌࡓᯒฟ≀ࡀẚ㍑ⓗᏳᐃ࡛࠶ࡿࡇ࡜ࡀ♧ࡉࢀ

ࡓࠋTDO⩌࡟࠾࠸࡚ࡣ㸪pHࢧ࢖ࢡࣝࡢ㈇Ⲵ࡟ࡼࡗ࡚㇟∳⣽⟶ࡢ㛤ཱྀࡀほᐹࡉࢀࡓࡀ㸪 TDR⩌࡛ࡣTDࢆ⧞ࡾ㏉ࡋሬᕸࡋࡓࡇ࡜࡟ࡼࡗ࡚ᐇ㦂ᮇ㛫୰ࡶ⣽⟶ࡢ㛤ཱྀࡣㄆࡵࡽࢀ

ࡎ㸪⤒᫬ⓗ࡟㇟∳㉁⾲㠃࡟࠾ࡅࡿᯒฟ≀ࡀⴭ᫂࡟ㄆࡵࡽࢀࡓࠋ

SEMほᐹࡢ⤖ᯝ࠿ࡽࡣ㸪Baseline࡟࠾࠸࡚ࡣ㇟∳⣽⟶ࡢ㛤ཱྀ࡜࡜ࡶ࡟⣽⟶ෆ࡟ࡣࢫ

࣑࢔࣮ࣉࣛࢢࡶㄆࡵࡽࢀ࡞࠿ࡗࡓࠋྛ᮲௳࡟࠾ࡅࡿ28᪥⤒㐣ᚋࡢヨ∦࡟࠾ࡅࡿSEM

ീ࠿ࡽࡣ㸪De⩌࡛ࡣ㇟∳⣽⟶ࡀ₃ᩯ≧࡟㛤ཱྀࡋࡓീࢆ♧ࡋࡓࡢ࡟ᑐࡋ࡚㸪Control ࡛ ࡣ㇟∳⣽⟶ࡣ㛤ཱྀࡋ࡚࠸ࡿࡶࡢࡢ㸪De ⩌࡜ࡣ᫂ࡽ࠿࡟␗࡞ࡿീࢆ♧ࡋ࡚࠸ࡓࠋTDO

⩌࡟࠾࠸࡚ࡣ㸪pHࢧ࢖ࢡࣝࡀ㈇Ⲵࡉࢀ࡚࠸࡞࠸Control࡜ẚ㍑ࡍࡿ࡜㇟∳⣽⟶ࡢ㛤ཱྀ

ࡀㄆࡵࡽࢀࡓࡶࡢࡢ㸪⣽⟶ࡢ୍㒊࡟ࡣᯒฟ≀ࡀṧ␃ࡋ࡚࠾ࡾ㸪ࡉࡽ࡟ TDR ⩌࡟࠾࠸

࡚ࡣ㸪⾲ᒙ࡞ࡽࡧ࡟⣽⟶ཱྀ࡟ᯒฟ≀ࡀ඘‶ࡋ࡚࠸ࡿീࡀほᐹࡉࢀࡓࠋࡉࡽ࡟㸪EDX ࡢ⤖ᯝࢆࡶ࡜࡟Ca/Pẚࢆ⟬ฟࡋࡓ࡜ࡇࢁ㸪Baseline࡜TDR⩌࡜࡛ᕪࡀ࡞࠿ࡗࡓ࡜ࡇ

ࢁ࠿ࡽ㸪ṑ㉁࡟㏆ఝࡋࡓࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࡀᙧᡂࡉࢀ࡚࠸ࡿࡇ࡜ࡀ♧၀ࡉࢀࡓࠋ ᮏᐇ㦂ࡢ⤖ᯝ࠿ࡽ㸪TD ࡢሬᕸ࡟ࡼࡗ࡚㇟∳⣽⟶ࡀࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺ࡟ࡼࡗ

࡚ᑒ㙐ࡉࢀ㸪ࡉࡽ࡟⧞ࡾ㏉ࡋሬᕸࡍࡿࡇ࡜࡟ࡼࡗ࡚㇟∳㉁⾲ᒙࡶᯒฟ≀࡛そࢃࢀࡿࡇ

࡜ࡀ♧ࡉࢀࡓࠋࡲࡓ㸪ඖ⣲ศᯒࡢ⤖ᯝ࠿ࡽṑ㉁ࡢᵓᡂᡂศ࡟ኚ໬ࡀ࡞࠸ࡇ࡜࠿ࡽ⏕య ぶ࿴ᛶ࡟ඃࢀࡿ࡜⪃࠼ࡽࢀ㸪⮫ᗋ࡟࠾ࡅࡿ᭷ຠᛶࡀᮇᚅࡉࢀࡓࠋ

⤖ ㄽ

ࣜࣥ㓟࢝ࣝࢩ࣒࢘⣔▱ぬ㐣ᩄᢚไᮦࡀ㸪㇟∳㉁ࡢ⬺⅊ᢚไ࠾ࡼࡧ㇟∳⣽⟶ᑒ㙐ᛶ࡟

ཬࡰࡍᙳ㡪࡟ࡘ࠸࡚㸪㉸㡢Ἴ㏱㐣ἲࢆᛂ⏝ࡍࡿ࡜࡜ࡶ࡟LSM࠾ࡼࡧSEMࢆ⏝࠸᳨࡚

ウࡋࡓ⤖ᯝ㸪௨ୗࡢ⤖ㄽࢆᚓࡓࠋ

1. ㉸㡢Ἴ㏱㐣ἲࢆ⏝࠸ࡓ㡢㏿ ᐃࡢ⤖ᯝ㸪De⩌࡟࠾࠸࡚ࡣ㡢㏿ࡢపୗࢆ♧ࡋࡓࡀ㸪 TDR⩌࡛ࡣ㸪᭷ព࡞㡢㏿ࡢୖ᪼ࡀㄆࡵࡽࢀ㸪pHࢧ࢖ࢡࣝࡢ㈇Ⲵ࡟ࡼࡗ࡚ࡶ㡢㏿ࡣ ኚ໬ࡋ࡞࠿ࡗࡓࠋ

2. LSMࢆ⏝࠸ࡓ⾲㠃ᛶ≧ࡢほᐹ࠿ࡽࡣ㸪TDO⩌࡛ࡣ㇟∳⣽⟶ࡢ㛤ཱྀീࡀほᐹࡉࢀࡓ

ࡶࡢࡢ㸪TDR⩌࡛ࡣ㇟∳⣽⟶ࡀᑒ㙐ࡉࢀ࡚࠸ࡓࠋ

3. SEMほᐹ࠿ࡽࡣ㸪Control㸪TDO࠾ࡼࡧTDR⩌ࡢ࠸ࡎࢀ࡟࠾࠸࡚ࡶ⣽⟶῝㒊࡟ᯒ ฟ≀ࡀほᐹࡉࢀࡓࠋࡲࡓඖ⣲⤌ᡂศᯒ࡛ࡣ㸪ṑ㉁ࡢᵓᡂᡂศ࡟ኚ໬ࡣㄆࡵࡽࢀ࡞

࠿ࡗࡓࠋ

ᩥ ⊩

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4) Chu CH, Lam A, Lo EC. Dentin hypersensitivity and its management. Gen Dent 2011;

59: 115-122.

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6) Cummins D. Advances in the clinical management of dentin hypersensitivity: A review of recent evidence for the efficacy of dentifrices in providing instant and lasting relief. J Clin Dent 2011; 22: 100-107.

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1059-1070.

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ᛂ⏝ࡋࡓ㇟∳㉁▱ぬ㐣ᩄ⑕ࡢ἞⒪࡟㛵ࡍࡿ◊✲. ṑᮦჾ 1989; 8: 282-294.

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855-860.

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Sadeghpour A, Nakamoto T. Remineralization of artificial enamel lesions by theobromine.

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⾲

⾲䛚䜘䜃ᅗ㻌

Group Treatment time (days)

0 7 14 21 28

Baseline 3775 (55)^a,A 3742 (55)^a,A 3750 (54)^a,A 3761 (57)^a,A 3764 (56)^a,A De 3785 (50)^a,A 3590 (49)^b,C 3524 (43)^b,C 3495 (44)^b,C 3462 (44)^b,C TDO 3781 (57)^a,A 3794 (65)^a,A 3733 (66)^a,A 3674 (65)^a,A 3664 (61)^a,A TDR 3772 (57)^a,A 3945 (65)^b,B 3990 (68)^b,B 3992 (62)^b,B 3990 (51)^b,B Control 3782 (57)^a,A 3942 (65)^b,B 3940 (68)^b,B 3921 (62)^b,B 3924 (51)^b,B Table 1 Ultrasonic velocities (m/s) of bovine dentin specimens

Values in parenthesis indicate standard deviations (N = 6).

Within groups, values with the same lower-case superscript letter are not significantly different (P > 0.05).

Between groups at the same treatment times, values with the same upper-case superscript letter are not significantly different (P > 0.05).

Fig. 1 Experimental set-up of the ultrasonic device for detection of tooth demineralization.

3400 3500 3600 3700 3800 3900 4000 4100

0 7 14 21 28

Baseline De TDO TDR Control

Fig. 2 Sonic velocities of bovine dentin under the different treatment conditions with different storage periods.

Time ( days )

Sonic velocity ( m/s )

Control

0 day TD application

7 days 14 days 28 days

TDO

TDR

Fig. 3 Laser scanning microscope observations of dentin surfaces.

Baseline De 28 days

Control 28 days TDO 28 days

TDR 28 days

Element Wt% At%

C O P Ca

21.8 29.0 17.3 31.9

36.4 36.3 11.2 16.0 Ca /P : 1.84

Baseline

C O P Ca

14.3 32.0 18.3 34.1

Element Wt% At%

26.8 42.1 13.8 16.4 Ca /P : 1.86

TDR 28 days