4 - Tues Junichi Ito.Commercialization

2016.10.25

Higashimaru Co.,Ltd.

Jun-ichi Ito

About us

—

Company name ： Higashimaru Co.,Ltd.

—

Year of establishment ： 1979

—

Head office ： Kagoshima pref., Japan

—

Number of employees ： 171

—

Major business area ： aquaculture feed, food

—

Common stock ： 603.9 million yen

—

Revenue ： 8,568 million yen (2015)

Head office

Formula feed

For larvae For prawn For fish Flied noodles Instant noodles Soup

Tokyo

Microalgae industry

Aurantiochytrium residue

Oil

・

_Squalene

・

_DHA

・

_Astaxanthin

Pharmaceuticals

Cosmetics

Chemicals

Health foods

Biofuel

Microalgae

Chapter 1

Chapter 2

Aurantiochytrium

Feed

(For protein)

(For DHA)

Today’s topic

Chapter 1.

Utilization of Aurantiochytrium residue as a protein

source for aquaculture

Chapter 2.

Application of algae as a DHA source for aquaculture

0 20,000 40,000 60,000 80,000 100,000 120,000 140,000 160,000 180,000

Fish meal

Fish oil

Binder

Vitamins

Minerals Others

Trends in aquaculture feed

Price of fish meal

Imported from Peru, Chile

yen/t

Increasing demand

Diet containing low or no fish meal

Is it possible to use algae as protein source of the diet?

Composition of fish feed

Aquaculture requires a lot of fish meal.

0 50 100 150 Thr Val Met Ile Leu Phe His Lys Arg 0 100 200 300 Thr Val Met Ile Leu Phe His Lys Arg 0 50 100 150 Thr Val Met Ile Leu Phe His Lys Arg

Fish meal Soybean meal

Corn

gulten meal Residue Moisture 8.7 13.2 9.6 12.9

Crude protein 66.0 44.2 59.6 71.5

Crude lipid 8.5 1.0 0.8 1.0

Crude ash 17.2 5.7 2.4 10.5 Hydrocarbon-extracted _{residue of Aurantiochytrium}

Same as fish meal

Essential amino acid profile comparing with fish meal (%)

Corn gulten meal

Soybean meal

Lack of methionine

Lack of lysine

Proximate analysis (%)

Add crystal amino acid

Comparison with major protein source

7 5 8 0 8 5 9 0 9 5 1 0 0 0 1 2 3 4 5 Co n tr o l R e s2 . 5 % R e s5 . 0 % Fe e d e ff ic ie n c y (% ) Da ir y f e e d in g r a te (% ) D FR FE 0 1 2 3 4 5 6 7 0 2 4 6 8 Me an b o d y w e ig h t（ ｇ） W e e k s Co n tr o l R e si d u e 2 . 5 % R e si d u e 5 . 0 % Rearing conditions

Treatments: control, residue 2.5%, residue 5％

Fish ：Kuruma prawn, mean body weight 0.4 g, 20 fishes / tank Tank ：100 L

Water ：filtered sea water（23.2～28.1℃） Feeding ：once a day, evening

Same performance among the tests

ⅰ）

_{Feeding trial with Kuruma prawn}

0 2 0 4 0 6 0 8 0 1 0 0 0 2 4 6 8 Me a n b o d y w e ig h t（ ｇ） W e e k s Co n tr o l R e si d u e 2 . 5 % R e si d u e 5 . 0 % 5 0 6 0 7 0 8 0 9 0 1 0 0 0 1 2 3 4 5 Co n tr o l R e s2 . 5 % R e s5 . 0 % Fe e d e ff ic ie n c y (% ) Da ir y f e e d in g r a te (% ) D FR FE Rearing conditions

Treatments: control, residue 2.5%, residue 5％

Fish ：Red sea bream, mean body weight 22 g, 15 fishes / tank Tank ：1 t

Water ：filtered sea water（23.2～27.6℃） Feeding ：twice a day, satiation

Same performance among the tests

ⅱ）

_{Feeding trial with red sea bream}

5 0 6 0 7 0 8 0 9 0 1 0 0 0 . 0 0 . 3 0 . 6 0 . 9 1 . 2 1 . 5 Co n tr o l R e s5 % R e s1 0 % Fe e d e ff ic ie n c y (% ) Da ir y f e e d in g r a te (% ) D FR FE 6 8 1 0 1 2 1 4 1 6 0 4 8 1 2 Me an b o d y w e ig h t（ ｇ） W e e k s Co n tr o l R e si d u e 5 % R e si d u e 1 0 % Rearing conditions

Treatments: control, residue 5%, residue 10％

Fish ：Abalone, mean body weight 8.2 g, 30 fishes / tank Tank ：1 t

Water ：filtered sea water（20.0～27.5℃） Feeding ：once a day, evening

Same performance among the tests

ⅲ）

_{Feeding trial with abalone}

・

Residue is excellent protein source for aqua feed

・

Residue can replace fish meal.

Summary

1.Utilization of Aurantiochytrium residue as a protein source

If algal residue could be adopted in all species...

Aquaculture feed 280,000 ｔ×5％＝14,000 ｔ

http://www.kusudama.jp/recipe/keyword/d0mt3h000000 http://zukan.com/fish/leaf13579 http://www.uoya.co.jp/uoyasake/how_awabi.html

Kuruma prawn

_{Red sea bream}

_Abalone

Today’s topic

Chapter 1.

Utilization of Aurantiochytrium residue as a protein

source for aquaculture

Chapter 2.

Application of algae as a DHA source for aquaculture

0 10 20 30 40 50 60 70 80 Japan China USA World India

Effect of DHA on human

Effects of DHA on human

・

Maintaining functional

development of the brain

・

Preventing cardiovascular

disease and cognitive deficit

Japanese government recommends taking

1 g/day

of EPA and DHA.

⇒

About

0.8 g/day

(average)

(kg/person)

Annual seafood consumption

（FAO：Food balance sheets, MAFF, Food Balance Sheet ）

Is it possible to enrich DHA content of seafood ?

What is DHA?

・

C22：6n-3 (ω3 fatty acid)

・

produced by marine microalgae

・

transfer to fish by bioaccumulation

4 5 6 7 8 9 10 0 2 4 6 8 M ea n bo dy w ei gh t ( g) Weeks Control DHA algae 2.5% DHA algae 5.0% 0.0 0.1 0.2 0.3 DHA c o n te n t ( % ) Control DHA algae 2.5% DHA algae 5.0% Rearing conditions

Treatments: control, DHA algae 2.5%, DHA algae 5％

Fish ：Kuruma prawn, mean body weight 4.5 g, 12 fishes / tank Tank ：100 L

Water ：filtered sea water（14.8～25.1℃） Feeding ： once a day, evening

DHA content

30%UP

×

1.5 DHA

×

2 .1 DHA

http://www.gardenhills.jp/shopping

ⅰ）

_{Kuruma prawn}

Same performance

250 270 290 310 330 350 0 2 4 6 8 M ea n bo dy w ei gh t ( g) Weeks Control DHA algae 2.5% DHA algae 5.0% 0.0 0.1 0.2 0.3 0.4 0.5 DHA c o n te n t ( % ) Control DHA algae 2.5% DHA algae 5.0% Rearing conditions

Treatments: control, DHA algae 2.5%, DHA algae 5％

Fish ：Red sea bream, mean body weight 275 g, 15 fishes / tank Tank ：1 t

Water ：filtered sea water（14.4～18.7℃） Feeding ：twice a day, satiation

ⅱ）

_{Red sea bream}

DHA content

20%UP

http://www.kensanpin.org/products/products/list.ph...

Same performance

Objective: to produce DHA enriched seafood

It is possible to produce seafood with high DHA content

by feeding algal DHA-rich diet.

30%

ＵＰ

20%

ＵＰ

Summary

2. Application of algae as a DHA source for aquaculture.

EPA+DHA intake

0.8 g/day×

1.3

＝

_1.04

_g/day

(dietary reference intake)

Conclusion

・

_{Microalgae are excellent protein sources for aquaculture because}

amino acid profile is similar to that of fish meal.

・

_{Algal oil-extracted residue can be blended: 5% in kuruma prawn feed}

and in red sea bream feed, and 10% in abalone feed.

・

_{It is possible to produce seafood with high DHA content by feeding}

algal DHA-rich diet.

We aspire to establish sustainable aquaculture system which contributes

to the health of society through the microalgae products in the future.

Acknowledgement

This work was supported by Council for Science, Technology and

Innovation(CSTI), Cross-ministerial Strategic Innovation Promotion

Program (SIP)

Funding agency : NARO.

Thank you for your kind attention.