Title
Improving the Utilization of Agricultural By-product as Feed for
Ruminant: Studies of Fermented Juice of Epiphytic Lactic Acid
Bacteria on Total Mixed Ration( 内容と審査の要旨(Summary) )
Author(s)
Yuli Yanti
Report No.(Doctoral
Degree)
博士(農学) 甲第705号
Issue Date
2019-03-13
Type
博士論文
Version
ETD
URL
http://hdl.handle.net/20.500.12099/77941
※この資料の著作権は、各資料の著者・学協会・出版社等に帰属します。㹙㸯㸲㹛 Ặ ྡ㸦ᮏ㸦ᅜ㸧⡠㸧 Yuli Yanti㸦ࣥࢻࢿࢩඹᅜ㸧 Ꮫ ࡢ ✀ 㢮 ༤ኈ㸦㎰Ꮫ㸧 Ꮫ グ ␒ ྕ ㎰༤⏥➨㸵㸮㸳ྕ Ꮫ ᤵ ᖺ ᭶ ᪥ ᖹᡂ㸱㸯ᖺ㸱᭶㸯㸱᪥ ◊ ✲ ⛉ ཬ ࡧ ᑓ ᨷ 㐃ྜ㎰Ꮫ◊✲⛉ ⏕≀⏕⏘⛉Ꮫᑓᨷ ◊✲ᣦᑟࢆཷࡅࡓᏛ ᒱ㜧Ꮫ
Ꮫ ㄽ ᩥ 㢟 ┠ Improving the Utilization of Agricultural By-product as Feed for Ruminant: Studies of Fermented Juice of Epiphytic Lactic Acid Bacteria on Total Mixed Ration 㸦ⱄᐙ␆⏝㣫ᩱࡋ࡚ࡢ㎰ᴗ⏘≀ࡢ⏝⋡ᨵ ၿ㛵ࡍࡿ◊✲㸸㎰ᴗ⏘≀TMR ࢧ࣮ࣞࢪ ᑐࡍࡿ╔ங㓟⳦Ⓨ㓝ᾮࡢຠᯝ㸧 ᑂ ᰝ ጤ ဨ ᰝ ᒱ㜧Ꮫ ᩍ ᤵ ᒣ ᮏ ᮒ ⨾ ᰝ ᒱ㜧Ꮫ ᩍ ᤵ ඵ௦⏣ ┿ ே ᰝ 㟼ᒸᏛ ᩍ ᤵ ➲ ᾉ ▱ ᏹ ㄽ ᩥ ࡢ ෆ ᐜ ࡢ せ ᪨ ✄ࢃࡽࡸࢺ࢘ࣔࣟࢥࢩ⛩࡞ࡢ㎰ᴗ⏘≀ࡣⱄᐙ␆⏝ࡢ㣫ᩱࡋ࡚ୡ⏺ⓗᗈࡃ ⏝ࡉࢀ࡚࠸ࡿࠋࡋࡋ࡞ࡀࡽ㸪ᰤ㣴౯ࡀపࡃ㸪ⱄື≀⤥ࡋࡓ㝿ࡣ ᬮ࢞ ࢫ࡛࠶ࡿ࣓ࢱࣥࡢᨺฟ㔞ࡀከ࠸ࡓࡵ㸪࢚ࢿࣝࢠ࣮⏝ຠ⋡ࡶప࠸ࡇࡀၥ㢟࡛࠶ࡿࠋ ᮏ◊✲ࡣ㸪㎰ᴗ⏘≀᫆Ⓨ㓝ᛶⅣỈ≀ࢆከࡃྵࡴ㣗ရ〇㐀⏘≀ࢆ⤌ࡳྜࢃࡏ㸪 ࡘಖᏑᛶࢆ㧗ࡵࡿࡓࡵΰྜ㣫ᩱ㸦TMR㸧ࢧ࣮ࣞࢪࡋ㸪ࡉࡽࢥࢫࢺࢆᢚ ࠼࡚ࢧ࣮ࣞࢪࡢရ㉁ࢆಖࡘࡓࡵ㸪స≀ඖ᮶╔ࡋ࡚࠸ࡿங㓟⳦ࢆᇵ㣴ࡋࡓ╔ ங㓟Ⓨ㓝ᾮ㸦FJLB㸧ࢆ⏝ࡍࡿࡇࡀ㸪㎰ᴗ⏘≀ TMR ࢧ࣮ࣞࢪࡢⓎ㓝ရ㉁ࢆᨵ ၿࡋ㸪ᐙ␆⏕⏘ࢆྥୖࡉࡏࡿ࠺ࢆ᫂ࡽࡍࡿࡇࢆ┠ⓗࡋࡓࠋ ᮏㄽᩥࡣ4 ❶࡛ᵓᡂࡉࢀ࡚࠸ࡿࠋ➨ 1 ❶࡛ࡣ㸪㎰ᴗ⏘≀ࡢ㣫ᩱᰤ㣴౯ࡢᨵၿ㛵 ࡍࡿ㐣ཤࡢ◊✲ࢆ≀⌮ⓗ㸪Ꮫⓗ࠾ࡼࡧ⏕≀Ꮫⓗᡭἲศ㢮ࡋ࡚⥲ㄝࡋ㸪ᐇ⏝ᛶ㸪Ᏻ ᛶ㸪⤒῭ᛶࡢⅬࡽ㎰ᴗ⏘≀ࢆTMR ࢧ࣮ࣞࢪࡋ࡚㸪Ⓨ㓝ရ㉁ᨵၿࡢࡓࡵ FJLB ࢆῧຍࡍࡿ᪉ἲࡀ᭷ᮃ࡛࠶ࡿࡇࢆ♧ࡋࡓࠋ ➨2 ❶࡛ࡣ㸪ᐇ㦂ᐊࢫࢣ࣮࡛ࣝ FJLB ࡢῧຍࡀ㎰ᴗ⏘≀࣭㣗ရ〇㐀⏘≀ TMR ࢧ ࣮ࣞࢪࡢⓎ㓝ရ㉁ᰤ㣴౯ཬࡰࡍᙳ㡪ࢆ㸪↓ῧຍ࠾ࡼࡧᕷ㈍ࡢங㓟⳦〇ẚ㍑ ࡋ㸪FJLB ࡢῧຍࡣ↓ῧຍ࠾ࡼࡧᕷ㈍ங㓟⳦〇ࡢῧຍẚ㸪 TMR ࢧ࣮ࣞࢪࡢங 㓟⃰ᗘࡀ㧗ࡵࡿࡇ࡛pH ࢆపࡃᢚ࠼㸪≀㸦DM㸧ࡢᦆኻࢆᑡ࡞ࡃࡋ㸪Ⓨ㓝ရ㉁ࢆᨵ ၿ࡛ࡁࡿࡇࢆ♧၀ࡋࡓࠋ ➨3 ❶࡛ࡣ㸪FJLB ࢆῧຍࡋࡓ㎰ᴗ⏘≀࣭㣗ရ〇㐀⏘≀ TMR ࢧ࣮ࣞࢪࡀࣄࢶ
ࢪ࠾ࡅࡿᰤ㣴⣲ࡢᾘ⋡㸪ⱄ⫶ෆⓎ㓝࠾ࡼࡧ❅⣲ฟ⣡ཬࡰࡍᙳ㡪ࢆ᳨ドࡋࡓࠋ ࡑࡢ⤖ᯝ㸪TMR ࢧ࣮ࣞࢪࡢⓎ㓝ရ㉁ࡣ↓ῧຍࡢሙྜ࡛ࡶ༑ศ㧗ࡃ㸪ࡑࡢࡓࡵᦤྲྀ 㔞࠾ࡼࡧᾘ⋡ᕪࡣㄆࡵࡽࢀ࡞ࡗࡓࠋࡲࡓ㸪FJLB ࡢῧຍࡣⱄ⫶୰ࡢࣥࣔࢽ ❅⣲⃰ᗘ࠾ࡼࡧⓎᛶ⬡⫫㓟㸦VFA㸧⃰ᗘࡶᙳ㡪ࡏࡎ㸪❅⣲ᦤྲྀ㔞㸪ἥ㔞࠾ࡼࡧ ✚㔞ࡶᙳ㡪ࡋ࡞࠸ࡇࢆ᫂ࡽࡋࡓࠋ ➨4 ❶࡛ࡣ㸪FJLB ࢆῧຍࡋࡓ㎰ᴗ⏘≀࣭㣗ရ〇㐀⏘≀ TMR ࢧ࣮ࣞࢪࡀࣄࢶ ࢪ࠾ࡅࡿ࢚ࢿࣝࢠ࣮ฟ⣡࠾ࡼࡧin vitro ἲࡼࡿ࣓ࢱࣥ⏕⏘㔞ࢆ᳨ドࡋࡓࠋ๓❶ྠ ᵝ㸪TMR ࢧ࣮ࣞࢪࡢⓎ㓝ရ㉁ࡣ↓ῧຍࡢሙྜ࡛ࡶ༑ศ㧗ࡃ㸪ࡑࡢࡓࡵ࢚ࢿࣝࢠ࣮ ฟ⣡ࡢᙳ㡪ࡣㄆࡵࡽࢀ࡞ࡗࡓࠋࡲࡓ㸪࣓ࢱࣥ⏕⏘㔞ࡶFJLB ࡢῧຍࡼࡿ᫂☜࡞ ຠᯝࡣ࡞ࡗࡓࡀ㸪㎰ᴗ⏘≀࣭㣗ရ〇㐀⏘≀ࢆTMR ࢧ࣮ࣞࢪࡍࡿࡇ࡛㸪 ࣓ࢱࣥࡼࡿ࢚ࢿࣝࢠ࣮ᦆኻࡣ㣫ᩱ⥲࢚ࢿࣝࢠ࣮ࡢ8㸣⛬ᗘᶆ‽ⓗ࡞⠊ᅖᢚ࠼ࡽ ࢀࡿࡇࡀ᫂ࡽ࡞ࡗࡓࠋ ௨ୖࡢࡇࡽࠊ㎰ᴗ⏘≀࣭㣗ရ〇㐀⏘≀TMR ࢧ࣮ࣞࢪ FJLB ࢆῧຍࡍࡿ ࡇࡣࢧ࣮ࣞࢪࡢⓎ㓝ရ㉁ࢆᨵၿࡍࡿྍ⬟ᛶࡀ♧၀ࡉࢀࡓࡀ㸪ங㓟⳦ࢆከࡃྵࡴ㣗 ရ〇㐀⏘≀ࢆΰྜࡋࡓሙྜࡣ㸪Ⓨ㓝ရ㉁ࡢᨵၿᑐࡍࡿຠᯝࡣ᫂☜࡛ࡣ࡞ࡗ ࡓࠋࡋࡋ࡞ࡀࡽ㎰ᴗ⏘≀ࢆTMR ࢧ࣮ࣞࢪࡍࡿࡇ࡛㸪࣓ࢱࣥ⏕⏘㔞ࡣᢚไ ࡍࡿࡇࡀྍ⬟࡛࠶ࡿࡇࡀ♧၀ࡉࢀࡓࠋ ᑂ ᰝ ⤖ ᯝ ࡢ せ ᪨ ✄ࢃࡽࡸࢺ࢘ࣔࣟࢥࢩ⛩࡞ࡢ㎰ᴗ⏘≀ࡣⱄᐙ␆⏝ࡢ㣫ᩱࡋ࡚㏵ୖᅜࢆ୰ᚰ ୡ⏺ⓗᗈࡃ⏝ࡉࢀ࡚࠸ࡿࠋࡋࡋ࡞ࡀࡽ㸪㎰ᴗ⏘≀ࡣ㣫ᩱࡋ࡚ࡢᰤ㣴౯ࡀప ࠸ୖ㸪ⱄື≀⤥ࡋࡓ㝿ࡣ ᬮ࢞ࢫ࡛࠶ࡿ࣓ࢱࣥࡢᨺฟ㔞ࡀከ࠸ࡓࡵ㸪࢚ࢿ ࣝࢠ࣮⏝ຠ⋡ࡶప࠸ࡇࡀၥ㢟࡛࠶ࡿࠋࡑࡇ࡛⏦ㄳ⪅ࡣ㸪㏵ୖᅜ࡛ࡶධᡭࡀᐜ᫆࡞ᰤ 㣴౯ࡢ㧗࠸㇋⭉⢑࡞ࡢ㣗ရ〇㐀⏘≀ࢆ㸪㎰ᴗ⏘≀ΰྜࡋ࡚㣫ᩱ㸦TMR㸧ࡋ㸪 ࡘࢧ࣮ࣞࢪࡋಖᏑᛶࢆ㧗ࡵࡿࡇࢆᥦࡋ㸪ࡉࡽࢥࢫࢺࢆᢚ࠼࡚ࢧ࣮ࣞࢪࡢ ရ㉁ࢆಖࡘࡓࡵ㸪స≀ඖ᮶╔ࡋ࡚࠸ࡿங㓟⳦ࢆᇵ㣴ࡋࡓ╔ங㓟Ⓨ㓝ᾮ㸦FJLB㸧 ࢆ⏝ࡍࡿࡇࡀ㸪㎰ᴗ⏘≀TMR ࢧ࣮ࣞࢪࡢⓎ㓝ရ㉁ࢆᨵၿࡋ㸪ᐙ␆⏕⏘ࢆྥୖ ࡉࡏࡿ࠺ࢆ᫂ࡽࡋࡼ࠺ࡋࡓࠋ ᮏㄽᩥࡣ4 ❶࡛ᵓᡂࡉࢀ࡚࠸ࡿࠋ➨ 1 ❶࡛ࡣ㸪㎰ᴗ⏘≀ࡢ㣫ᩱᰤ㣴౯ࡢᨵၿ㛵ࡍ ࡿ㐣ཤࡢ◊✲ࢆ≀⌮ⓗ㸪Ꮫⓗ࠾ࡼࡧ⏕≀Ꮫⓗᡭἲศ㢮ࡋ࡚⥲ㄝࡋ㸪ᐇ⏝ᛶ㸪Ᏻᛶ㸪 ⤒῭ᛶࡢⅬࡽ㎰ᴗ⏘≀ࡼࡾTMR ࢧ࣮ࣞࢪࢆㄪ〇ࡍࡿࡇ㸪ࡉࡽⓎ㓝ရ㉁ᨵ ၿࡢࡓࡵFJLB ࢆῧຍࡍࡿ᪉ἲࡀ᭷ᮃ࡛࠶ࡿࡇࡀ♧ࡉࢀ࡚࠸ࡿࠋ ➨2 ❶࡛ࡣ㸪ᐇ㦂ᐊࢫࢣ࣮࡛ࣝ FJLB ࡢῧຍࡀ㎰ᴗ⏘≀࣭㣗ရ〇㐀⏘≀ TMR ࢧ ࣮ࣞࢪࡢⓎ㓝ရ㉁ᰤ㣴౯ཬࡰࡍᙳ㡪ࢆ㸪↓ῧຍ࠾ࡼࡧᕷ㈍ࡢங㓟⳦〇ẚ㍑ࡋ㸪 FJLB ࡢῧຍࡣ↓ῧຍ࠾ࡼࡧᕷ㈍ங㓟⳦〇ࡢῧຍẚ㸪 TMR ࢧ࣮ࣞࢪࡢங㓟⃰ ᗘࢆ㧗ࡵࡿࡇ࡛pH ࢆపࡃᢚ࠼㸪ࡇࢀࡼࡾ≀㸦DM㸧ࡢᦆኻࡀ࡞ࡃ㸪Ⓨ㓝ရ㉁ࢆᨵ ၿ࡛ࡁࡿࡇࢆ♧၀ࡋࡓࠋ ➨3 ❶࡛ࡣ㸪FJLB ࢆῧຍࡋࡓ㎰ᴗ⏘≀࣭㣗ရ〇㐀⏘≀ TMR ࢧ࣮ࣞࢪࡀࣄࢶࢪ ࠾ࡅࡿᰤ㣴⣲ࡢᾘ⋡㸪ⱄ⫶ෆⓎ㓝࠾ࡼࡧ❅⣲ฟ⣡ཬࡰࡍᙳ㡪ࢆ᳨ドࡋࡓࠋࡑࡢ
⤖ᯝ㸪TMR ࢧ࣮ࣞࢪࡢⓎ㓝ရ㉁ࡣ↓ῧຍࡢሙྜ࡛ࡶ༑ศ㧗ࡃ㸪ࡇࢀࡣ㣗ရ〇㐀≀ ࡶࡶྵࡲࢀࡿங㓟⳦ࡢᙳ㡪ࡀࡁࡗࡓྍ⬟ᛶࢆ♧၀ࡋ࡚࠸ࡿࠋࡑࡢࡓࡵᦤྲྀ㔞 ࠾ࡼࡧᾘ⋡ࡶᕪࡣ࡞ࡃ㸪ࡲࡓ㸪FJLB ࡢῧຍࡣⱄ⫶୰ࡢࣥࣔࢽ❅⣲⃰ᗘ࠾ࡼ ࡧⓎᛶ⬡⫫㓟㸦VFA㸧⃰ᗘࡶᙳ㡪ࡏࡎ㸪❅⣲ᦤྲྀ㔞㸪ἥ㔞࠾ࡼࡧ✚㔞ࡶᙳ㡪 ࡋ࡞ࡗࡓࡇࢆ᫂ࡽࡋ࡚࠸ࡿࠋ ➨4 ❶࡛ࡣ㸪FJLB ࢆῧຍࡋࡓ㎰ᴗ⏘≀࣭㣗ရ〇㐀⏘≀ TMR ࢧ࣮ࣞࢪࡀࣄࢶࢪ ࠾ࡅࡿ࢚ࢿࣝࢠ࣮ฟ⣡࠾ࡼࡧin vitro ἲࡼࡿ࣓ࢱࣥ⏕⏘㔞ࢆ᳨ドࡋࡓࠋ๓❶ྠᵝ㸪 TMR ࢧ࣮ࣞࢪࡢⓎ㓝ရ㉁ࡣ↓ῧຍࡢሙྜ࡛ࡶ༑ศ㧗ࡃ㸪ࡑࡢࡓࡵ࢚ࢿࣝࢠ࣮ฟ⣡ ࡢᙳ㡪ࡣㄆࡵࡽࢀ࡞ࡗࡓࠋࡲࡓ㸪࣓ࢱࣥ⏕⏘㔞ࡶFJLB ࡢῧຍࡼࡿ᫂☜࡞ຠᯝࡣ ࡞ࡗࡓࡀ㸪㎰ᴗ⏘≀࣭㣗ရ〇㐀⏘≀ࢆTMR ࢧ࣮ࣞࢪࡍࡿࡇ࡛㸪࣓ࢱࣥ ࡼࡿ࢚ࢿࣝࢠ࣮ᦆኻࡣ㣫ᩱ⥲࢚ࢿࣝࢠ࣮ࡢ8㸣⛬ᗘᶆ‽ⓗ࡞⠊ᅖᢚ࠼ࡽࢀࡿࡇࢆ ᫂ࡽࡋ࡚࠸ࡿࠋ ࡇࢀࡽࡢ▱ぢࡣ㸪ⱄᐙ␆⏝ࡢ㣫ᩱࡋ࡚㎰ᴗ⏘≀ࡢ⏝ᨵၿࢆ⾜࠺ࡓࡵࡣ㸪㣗 ရ〇㐀⏘≀ΰྜࡋ࡚TMR ࢧ࣮ࣞࢪࡍࡿࡇࡀ㸪㣫ᩱရ㉁ࡢᨵၿ࠾ࡼࡧ࣓ࢱࣥ ⏕⏘㔞ࡢపῶࡢⅬࡽ᭷ຠ࡛࠶ࡾ㸪ࡘFJLB ࢆῧຍࡍࡿࡇ࡛ TMR ࢧ࣮ࣞࢪࡢⓎ 㓝ရ㉁ࢆᨵၿࡍࡿྍ⬟ᛶࢆ♧၀ࡍࡿࡶࡢ࡛࠶ࡿࠋᮏ◊✲ࡢ⤖ᯝࡣ㸪ࡃ㎰ᴗ⏘≀ࢆ ⱄᐙ␆⏝ࡢ㣫ᩱࡋ࡚ᗈࡃ⏝ࡋ࡚࠸ࡿ㏵ୖᅜࡢᐙ␆⏕⏘ᐤࡍࡿࡶࡢ࡛࠶ࡿࡇ ࢆㄆࡵࡿࠋ ᇶ♏࡞ࡿᏛ⾡ㄽᩥ
1) Yanti, Y., M. Yayota: Agricultural By-products as Feed for Ruminants in Tropical Area: Nutritive Value and Mitigating Methane Emission. Reviews in Agricultural Science, 5, 65-76, 2017.
2) Yanti, T., S Kawai., M. Yayota: Effect of total mixed rations containing agricultural by-products with the fermented juice of epiphytic lactic acid bacteria on rumen fermentation and nitrogen balance in ewes. Tropical Animal Health and Production, in press
