狂犬病ウイルス小松川株の遺伝学的および生物学的性状に関する研究
全文
(2) }0Zl\p ´7U
(3) |Ua~ º. 2020 ^ [»SUSU½³KFU Á[»SUÂ ¿ t À s.
(4) }0Zl\p ´7U
(5) |Ua~ º. ¿ t À s.
(6) u. «3¥. 1. §. 2. 1 }0Zl\p ´7Ua~ q©. 10. _¬. 11. kfH
(7) hy. 13. 1-1. O¾. 13. 1-2. 0. 13. 1-3. RNA d@. 15. 1-4. ²¯? PCR. 16. 1-5. u4521¦m. 17. 1-6. &-jQPµC xW RNA ~E
(8) RT-PCR. 18. 1-7. 12y 21¦m. 19. 1-8. ATU¦m. 20. 1-9. La¦m. 21. 1-10. J0¢® ,&·µC w°. 21 22. n 1-1. }0Zl\p &-rc. 22. 1-2. Zl\p5¤ }0p ATU. 22. º:a q©.
(9) 1-3. Zl\p RV303 p &-rc
(10) J´7T La q©. 23. 1-4. Zl\p
(11) 5¤ 0p N ¢®. 24. ,&·µC w° 1-5. Zl\p
(12) 5¤ 0p P ¢®. 25. ,&·µC w° 1-6. Zl\p
(13) 5¤ 0p M ¢®. 25. ,&·µC w° 1-7. Zl\p
(14) 5¤ 0p G ¢®. 26. ,&·µC w° 1-8. Zl\p
(15) 5¤ 0p L ¢®. 27. ,&·µC w° X. 29. N¤. 36. 2 }0Zl\p |Ua~ q©. 56. _¬. 57. kfH
(16) hy. 59. 2-1. O¾
(17) 0. 59. 2-2. )2". 60. 0bo8 zW. 2-3. NA Jp Rv q©. 61. 2-4. NA Jp cell-to-cell bo q©. 61. 2-5. J0p Ga q©. 62.
(18) 2-6. J0p 9=a q©. 62. 2-7. J0pbo+ {6i¹ q©. 63. 2-8. bo+ 0 A]. 63. 2-9. ¨¦m. 64. n. 66. 2-1. NA Zl\p Rv. 66. 2-2. NA Zl\p cell-to-cell bo. 66. 2-3. Zl\p Ga q©. 67. 2-4. Zl\p 9=a q©. 68. 2-5. Zl\pbo+ {6i¹. 68. 2-6. Zl\pbo+ 0 A]. 69. X. 71. N¤. 76. 3 }0Zl\p /'2%#y . 84. _¬. 85. kfH
(19) hy. 87. 3-1. O¾H
(20) 0. 87. 3-2. V>¸&-*.,$ pKoma r. 87. 3-3. V>¸&-*.,$ pKoma boa0 MI. 89. 3-4. £;<o¡ boa0 MI ª. 90. 3-5. rKoma p &- Y=´7T+22 ª . 90.
(21) RT-PCR 3-6. ´7T+22 ª D¼¶ Bg(!21 ¦m 91 3-7. ´7T+22 ª 21¦m. 91. 3-8. NA rKoma p Rv q©. 91. 3-9. NA rKoma p cell-to-cell bo q©. 92. 3-10. rKoma p Ga q©. 92. 3-11. rKoma p 9=a q©. 92. 3-12. ¨¦m. 93. n. 94. 3-1. boa0 MI ª. 94. 3-2. rKoma p&- Y=´7T+22 ª. 94. 3-3. NA rKoma p Rv. 95. 3-4. NA rKoma p cell-to-cell bo. 96. 3-5. rKoma p Ga q©. 96. 3-6. rKoma p 9=a q©. 96. X. 98. N¤. 101. ¬. 110. ±. 112. `e. 113.
(22) ÏUÍ. BSL. B'+4TD;&OGN. E-MEM. 'T/N|Ùsq. FCS. (2ÄbË. FFU. D*T,3h_. FITC. DN+P4'RS'68+2%AT=. GM. uªsq. IFN. 'R7TD)PR. LD50. 50% ÈÕ. MM. ¿ªsq. MOI. vÔ. NA ¼Å. I(3·½ÊÆ«¼Å. ORF. +TFRSMT<&R/SDOTJ. PBS. MRÓÁÌ©¨Út. RIG-I. Retinoic acid-inducible gene I. RT j. ÑÐej. 1.
(23) ÀÎ ¥£®ßÔ»·½¯¤ 100%Ûȧ$¢ "('N3 \¦dÒ¯!ßC=$mcoYg¡®Â "¥£® ZØf³Q.8Rxp "Uß®{ "f² ßµº #®®i`"¥£®('N3ÝLE>(' N3¸M91('N3}ÞßzXr$ß'@ß%L'/I-:A Úôg¡ß. l¹0(KM$®i~ÝO5NH%Þ
(24) Ç ¬. x¾
(25) "(29)C=ß #. g¡ na$k " ('N3.
(26) ßÖWy^×ÝÒ 2090 ß# 6 ]VÞ(30, 61)$½ ßÉwßܱ°·½¯¤$¶
(27) [ "¥£®ßß +4%?%3,R%C`UÐqr$×c$¬8a
(28) !(69)ߢßQ.8RB8{D
(29) ß%%ß%DM, _ÊVR$&iצ 59,000 \®"(18) ¤!ßQ.8Rt¹{D>ߥ£®²Ô®;S I Ô½ªjÅÝ". #ߥ£®²µº$´s
(30) ß A²6¬Ã¸ #
(31)
(32) ß #B8fµÄ # ß UÐ ©Ï/Q.8Rt¹"®Öy #(31) &ß 2004 %!M,ßQ.8Rt¹¥£®®ßÜѶp( 'N3¶$.ª "²!©Ï
(33) '/VK #(71)ß N( *T-TSFP=0NMH #ß #W®¥£® { "²
(34) g #
(35)
(36) ßG 6¬!
(37) ¯/VÂ. 2.
(38) ]X 1 /"{
(39) (51)ß6¬$k [
(40) ®^ 53 /VK #" . ßfO#"(31). ¤!ß²µºI p§ #" ß
(41) µº´6º b ߥ£®Ö^×&Ø&³Q.8Rt¹$]x "ß "|Ù3!®¯$Ø E¯" |Ù3 ³3"TN
(42) g¡. na|Ù$k eß. . |Ù 3ß7ß14 28 ´À 5 P!Q.8R$t¹ " u Z #"(70)Ôna!YÕ('N3|Ù$k "W Fߥ£®('N3{ "3/PEMR9PQ.8Rt¹zo# " ½"(70) |Ù3ÛfÄ. #. "ߥ£®X¸R"%%ß%DM,_ÊVR ßÛ1Q.8RÜPt¹3/PEMRt¹e½³Æ q½ #"ß{DB8Τ"(18)
(43) ß ¥£®²µºGz߸|Ù3$º?S*} "ß!\1 fÛ¯ZØÔ. #" w¯Z. زÔߥ£®('N3®icÕ "U kVµ½" ¥£®ßg¡N&xp "¥£®('N3na$)
(44) " !+v "a$)
(45) `524
(46) ('N3ߢè« u$½~·½
(47) ß
(48) ɧ5$ig " ß&·½ ¥:Ì "
(49) ß&·½¥rY
(50) ©ßÔ»·½¯¤. 3.
(51) $,ȳÚ@³±$dÈ (30) ¥£®('N3` 5glß~¨«. &·½¥rY "('N3¯=. ·½24ß&·½¥rY
(52) ßzXȳ·½¯¤$d È ('N3¯=·½®iU´³L#"('N3 ·½24·½®iß #¥£®®lWE" ß%® "l('N3·ÇÔ½f<$8[OGN¿y " ß²w¾¯ZØÔ "Ô½Uk Vh #". #". ¥£®('N3Jߦ 12,000 tUÚ8£I'3ÓUÓ RNA !ß3'~¡. NßPßMßG L Í+[ÛßË·ÇÝ
(53). # # NßPßMßG L ·ÇÞ$0T>
(54) "(32)('N3¤[ß NßPßL ·ÇJ RNA . "@.O+,F2>ßMßG ·. ÇzX¼Å«°Ç(Ô². "RGPTF #". (32)N ·ÇJ RNA ©F
(55) ß@.O+,F2>Þ" N-RNA ¼F`$ "(32)L ·ÇßRNA 0x RNA M!LT5
(56) ß
(57) dQ[" P ·ÇßN-RNA ¼F`$ÒT
(58) J RNA ¼» mRNA Ðe$¸(7, 14, 32)°Ç(Ô²$¹n
(59) " M · Çß('N3¤[7ÊÔ½f<$ (32)°Ç²XÐ 3'.¤ 7 " G ·ÇßzX¼Å('N3J24 Õ# "(32)Uß #. ('N3·ÇßNßP M ·Ç. ß('N33PίÕ# " y. "(5, 44, 45, 62,. 67, 68) ßc·Ç('N3¼»3PÎÙf<. 4.
(60) $q" . ß #·Ç-. icÕ#
(61) ". c$Ò ('N3®. #". ¥£®('N3®ic "l('N3·ÇÔ½ f<ß # m$ªÁ #/ß 1980 *&Õ. 1990 *9×ß&3K.PTNp`!Ç. T #¹&3¥:i¿g!ߥ£®('N3 G ·Ç· ½24·½®i%®Ô½f<$q y. . (10, 56)1994 ß.PTR@ #('N3J cDNA . (. 'N3$P. "S«ß"MBT3)A;&.3¥£®('N3 9µº #(58)¥£®('N3JQZ:$E¯ "W!ߥ£®('N3®icÕ "Ú» ³Â_
(62) $`³ßª!ß('N3 G ·Ç·½®i Ô½" %µÄ #(12, 63, 64)ßNßP M ·Ç ß #. "Ç 3S%=T23Pί$Ò ß·½®iÕ. # " VK #"(22, 24, 44, 45, 49). ßP ·Çß
(63) Ç. 3Í@x$)
(64) ß·½24Ô½f<$q y #(54, 75) #. . ߥ£®('N3®i "l·ÇÔ. ½$yµ¶
(65) ß®ic$8[OGN¨¿ "VÔ½ ¯$V
(66) Uß ¯ß¥£®('N3Ç / "®i c$
(67) j`
(68) E¯"Ç "Gc$y "ßÊX¸iÝ"¬VoiÞ$ª®i¿g. 5.
(69) Ù"{
(70) ßV¯YC]Ý?iSQ.8RiÝ"8yo iÞ¿gh. #"U´³ß8yoi߬Voi$(1 . I(3±ÖÁ¾* " jº #"ß jº #8yoi$l¹Ú·½¥sÛ¼Åß. Á¾*$. ¸ G¼ÅÛÜ@
(71) y©T8yoiµº #" *©ß8yoi߬Voi"Ç$ " "/ß·½¥sۼŠ"uf§y¤. #. #"·. ½°3߬Voi!ß8yoiÛ y "(59)ß8yoi·½24߬Voi!©
(72) . #. "(59, 74)Ò߬Voi$/!RßB'+4TD;&OGN ÝBSLÞ3 ]Ý?½"{
(73) ߬Voi!·½24$ "8yoißBSL2 ]Ý?/!RE¯" ß /!RB_"8yoi$ª®i¿gx¸#ß® icÕ "Ô½¯v\h. #Uß #. ¯$. ¥£®²Ôª "WF߬VoiG ¯h #"$µÄ " . #" ß®²$Ô. "ß8yoi ¬Voi®i¿g$Ò ß
(74) ®i c$8[OGNy. "½". ÊXßÍ+³"¥£®('N3ß g¡¹! # "¿ #ß
(75) # #ÇÂ@
(76) "(29) ßl ('N3ßÂ@Ä8Ç$h
(77) . ß
(78) x¾Ù". Ô½Ç$
(79) "ZP"¥£®('N3ßzX`ȳ. 6.
(80) ·½¯¤ß #ÕÁ
(81) [X¸g$µ " [=('N3$m
(82) ßU³®icc¬Voix #" . #"
(83) ßc¥£®¯/f²$Ô ". ß Í+<³S©¡<³¤$ "¼\¬Voi¿g$Ò ßc ¥£®('N3{ó®ic$y $¯7 ½"
(84)
(85) .
(86) ßbÔ³. ߬Voi®icÕ "8[O. GNkVßB8µ #"΢߼\¬Voi© ¡<³¤$¢³p¼SÁ
(87) ^. <³. ß #®i¿gª !¯. #"¾ßTroupin. #¬Voiߥ. ß$¬&8Ñ #¬Voi. l¹Q.8RißFÀ 321 icJÈ'U8[¥<³¿g$¸ ß #. ('N3i 8 ¥£ÝCosmopolitanßAfrica 2ßAfrica 3ß. Arctic-RelatedßIndian subcontinentßAsianßRAK-SK BatsÞ8Ù #" $VK
(88) (66)UßMBT3)A;&.3µº #ß ® i 8 [ c ¿ g E ¯ " ¬ V o À 7 i ß RABV-Dog i (53) ß RABV-Fox i (53) ß 1088 i (21) ß 8743THA i (34) ß GD-SH-01 i(65) SHBRV-18 i(11)ßV 8 ¥£ 3 ÝCosmopolitanßAsian BatsÞ}
(89) "]V!ß 5 ¥£ÝAfrica 2ßAfrica 3ßArctic-RelatedßIndian subcontinent RAK-SKÞ} "¬VoiÍ+<³©¡<³¤$¿y
(90) ß. #. ß. MBT3)A;&.3$µº " ßc¬Voi{Ã. ³®icÕ "Ô½¯$h" ". 7. #".
(91) §a&ßß]*ße8Ñ #¥£®( 'N3|fFi´
(92) iߥ£®YÌ #" 1957 ]* 1940 *ß½hA©ÃÝWuJO,9' @ 8Ñ #(3, 25, 50)
(93) ß|fFißx "6U«¬ Voi
(94) ß ¥£®xª #(2, 3, 19, 42, 50)/ß |fFi N Í+[È'U8[¥<³¿g. ß('N3i¥. £ Arctic-Related }
(95) ßP2%v{qrg¡C= N3i¾ " y. 8Ñ #('. " (3) # ß ¥ £ . Arctic-Related } "¬Voi®i´¼Á # Wy v . ß. (36)ß|fFi$ª®i¿g$]x " ß. ¬Voi{ó®ic$¿y "Ô½m!h. #. "g" Uß|fFiÍ+<³©¡<³¤kVßB8 h #"Î/ßiJtUÈ'ßN Í+[]X È'¿¶"ßcJÈ'Ußi´¼È$Á
(96) VKÍ+<³¢ $y.
(97) VKßi®i
(98). ©¡<³¤ß #`¥³Á # ¢ ß|fFiß'@. 8Ñ #ßKNK9=I(3. Á¾*!¿ #$`³ß¦it¹!¯
(99) KNK9=±c^$)`¦t¹ "±-~¨«*$ 19 P¡!¿
(100) (50)ßI(3 " 20 P]V±*]x #"
(101) ß *!ß|fFi¬Voi
(102) Ç$;. 8.
(103) ß8yoiÙ
(104) ©¡<³¤$h
(105) E¯1y]Ðß Arai. ß *. ß|fFi$8yoi
(106) 8Ù
(107). "(2)]V!ß|fFi©¡<³¤$´¼¿y " ß®i ¿g "iªy. " ߬Voi8y. oi©¡<³¤ÅÕ "É2|¯h. #".
(108) ߥ£®('N3|fFi¥<³È´ ¼¯$h" ß. ®i¿g "iª$y. ". $´³
(109) ßiÍ+<³©¡<³¤$¢³Á
(110) 1 ßk$*2T.R1T$ª|fFicJtUÈ'$sy
(111) ßi('N3i8[¥<³Õß ¢ $y. iÍ+<³.
(112) 2 ßin vitro in vivo ]Ý¥!|. fFiß*®³¬Voi8yoi©¡<³¤Ù SÅ $y " ß®i¿g "|fFiª$Á
(113) 3 ß®i¿gS«³U"MBT3)A;&.3$|f Fiµº "ß('N3J cDNA . |fFiÝrKoma iÞ. $P.
(114) ßiM|fFiÝÊ©TÞ©¡<³¤$p¼SÁ
(115) . 9.
(116) 1. ¥£®('N3|fFiÍ+<³¤Á. 10.
(117) c¸. #|fFii N Í+[U8[¥<³¿g !ß|fFi¥£ Arctic-Related }
(118) ßP2%v{qrg¡ C=. 8Ñ #('N3i¾ " y. #(3, 37)
(119).
(120) ßN Í+[]XJtUÈ'¿¶"ßiÈ$´¼ Á
(121) #]x #¢ßÇ ¬ß"¥ £®('N3i×JG¨W$K¶5 "VKxp " (20, 41)ß|fFiÈ$!lµuy "ßcJÈ'U ¥<³¿g½. #". ß|fFicJÈ'¿¶" ße ¢³Í+<³¿g]xÍ|fFißÊX'@. ßi 8Ñ. #ßKNK9=±-~¨«*ß. I(3±*!¿. #ßÒ¬VoiÄ. #v\:$JV.
(122) "E¯" :µ!ß|fFi¬Voi
(123) ©¡<³¤$;ß
(124) 8yoiÙ
(125) ¤$h
(126) E¯ 1y #ߥ£®('N3®i
(127) ©¡<³ ¤ß. l('N3·Ç¯LÔ$D %. y #" #. ÓmUG. kVUßiJ$¿g " ß('. N3i©¡<³¤$uy " ". #". 1 ß|fFiÈ$´¼uy " ß. i¬Voi.
(128) ¤$
(129) "$Í+<³!Ó. ³ " $´. ³
(130) ßicJÈ'$k$*2T.R3¿g!sy
(131) s. 11.
(132) y
(133) GÈ'Uß|fFi('N3i¥<³Õ$y
(134) Vß|fFicJÈ'$ß*®³¬Voi8yoi JÈ'¢³p¼Á
(135) . 12.
(136) c^ 1-1. sÛ¼Å. I(3·½Ê¼ÅÆ« NA ¼Å(46)$ 37²y
(137) CO2 'R-"GT 7TÝCL1QßeßÞ5sÛ
(138) Ý5% CO2ß95%qÞNA ¼Å uªsqÝGMÞ
(139) ß10%(2ÄËÝFCSÞ Ý»40: F7524ßSigmaß %!M,ß. TMGÞ~>'T/N MEM sqÝE-MEMÞÝ»40: 051-. 07615ßC9D&NJßßeÞ$ª¿ªsqÝMMÞ
(140) ßGM ~> " FCS Õ$ 5% $ª
(141) . 1-2. ('N3. ÊX'@. 8Ñ #|fFißKNK9=* #ßI(3. $ª±*!¿ #(50)ß|fFi3=9.( 'N3»"ºß24 *´I(3±Y+$ªÖ¬HÉ "('N3.PT?R/$¸$`³ßV±Y+$ß1,400 ×gß 10 8×ß?dÆi
(142) ß
(143) V$ MM 2 ÏnHÉ
(144) ß 20 ×106 ~ 26 ×106 HÉ('N3$ªQ
(145) kß96 ()N¨«sÛF OT=Ý»40: 655180ßGreiner Bio-Oneß+T3=M%ß.OJ3. "R. 37TÞVhEsÛ # NA ¼Å$ R.3Ý»40: 05905ßNissuiß ßeÞwz
(146) ßVÂHÉ('N3 25 µL $l()Nt¹
(147) Ðß1 HÉ!À 24 ()N NA ¼ÅHÉ('N3$t¹
(148) ('N3$¼ÅJ "ß37²y
(149) CO2 'R-"GT7T ÝCL1QÞ Ý5% CO2ß95%qÞ& 1 z×sÛ$¸ß% R.. 13.
(150) 3ÝNissuiÞ¼Å$wz
(151) ß100 µL GM $l()N>kß 12 ×sÛ
(152) ßl()NsÛ$cP.
(153) ß-80x
(154) ¾ßl()N NA ¼Å "('N3$(y "ß VÂsÛP.¼Å$ªß×tª#p`"('N3pi 7$¸ ß¼Å$8y "ßl()N 100 µL 4ÞL N J%N<C>-MRÓÁÌÝ»40: 163-20145ßC9D&NJÞ$>ß ßz×$¸kß²ÀÄ6¨$´³
(155) ßl()N 50 µL 100Þ!7TNÝ»40: 137-01823ßC9D&NJÞ$>ß1 8×ß¼Å
(156) ßMRÓÁÌ©¨ÚtÝPBSÞ! 4,000 HÉ #p¥£®('N3 N ·ÇK.PTNp` 13-27(48)$ 50 µL ( )N>ß37ß1 z×d¼Åj PBS ¼Å$wz
(157) ß2,000 HÉ #DN+P4'RS'68+2%AT=ÝFITCÞ¹ pI(3 IgGÝ»40: 55514ßCappelß%!M,ß,MD*N?%GÞ$ 50 µL ()N>ß37ß1 z×dj$¸% PBS ¼ Å$wzßAxiovert 200 ØNËÝCarl Zeiss Microscopy Co., Ltd.ß>':ß +TBT09
(158) RÞ$ª¼Å$±D
(159) ßl()N "('N3pi $(y
(160) 1 HÉ('N3$t¹
(161) À 24 ()N 10%]ß 2 ()N]piÎHɧxp " $µÄ
(162) ß
(163) Î ()N. P. #sÛ$ª%ßVÂÖ¬HÉ$¸2 P. ´Ö¬HÉßP´G ß()NpiΧ 10%] Hɧxp " $µÄ
(164) Vß
(165) Î()N. h. #sÛ. $.PT?R/('N3
(166) Â3=9.('N3»ª
(167) . 14.
(168) .PT?R/ #|fFi3=9.('N3$ß]mÛM »
(169) ßMM $> 500 µL ·]
(170) .PT?R/('N3 $ß. R.3ÝNissuiÞwz
(171) 6 ()N¨«sÛFOT=Ý». 40: 657160ßGreiner Bio-OneÞV NA ¼Åt¹
(172) 37²y
(173) CO2 'R-"GT7TÝCL1QÞÝ5% CO2ß95%qÞ& 1 z×sÛ $¸ß% R.3ÝNissuiÞ¼Å$wz
(174) Vß6 mL GM $ ()N>¼Å$ 10 ×sÛ
(175) ß
(176) sÛ$P.
(177) ß1,400 ×gß 10 8×ß?dÆi
(178) h. #V&('N3$. uI ". ß V$% 6 ()N¨«sÛFOT=V NA ¼ÅVÂG t¹
(179) ß¼Å$ 14 ×sÛ
(180) sÛ$P.ß1,400 ×gß10 8×ß?dÆi$¸ßh #V$3=9.('N3
(181) 80x
(182) ß$ "|fFi/!Rß BSL3 ]Ýx²& ¸. 1-3. RNA U7. k$*2T.R3¿gª "|fFiJ cDNA $F " ßGi3=9.('N3. J RNA $U7
(183) ß250 µL . 3=9.('N3 750 µL TRI Reagent LSÝ»40: TS120ßMolecular Research Center, Inc.ß%!M,ß+ '+GÞ$>ß }F$ 5 8 ×ß?Ò¬
(184)
(185) ß }F 200 µL .PP NJÝ»4 0: 034-02603ßC9D&NJÞ$~>
(186) ß12,000 ×gß15 8×ß4d. 15.
(187) Æi$¸Æi8Ñ #ßRNA $mVE$P.
(188) ß 500 µL '6FPTNÝ»40: 166-04836ßC9D&NJÞ$~>
(189) Vß }F$ 12,000 ×gß8 8×ß4dÆi
(190) ÆiV$× -
(191) t 1 mL 70% 7TNÝ»40: 057-00456ßC9D&N JÞ$~>
(192) ß. 7,500 ×gß5 8×ß4dÆi$¸V$>. c×-ßt{
(193) 5.0 µL RNase Wm¨ºÝ»40:10977-015ß Invitrogenß%!M,ß,MD*N?%GÞ$>" ßRNA $»
(194) . 1-4. ÑÐe PCR. U7 #VÂJ RNA $ÒT
(195) ßÑÐeÝRTÞj" 1 Ó cDNA F$¸$`³ß4.5 μL RNA 6.5 µL º $>VßRandom HexamersÝ50 µMÞ Ý»40: N8080127ßInvitrogenÞ dNTP MixÝ10 mMÞÝ»40: 18427013ßInvitrogenÞ$ 1.0 μL ~>
(196) }F$ 65 5 8×>
(197) r&j&
(198) ß 5×First-Strand Buffer $ 4.0 µLß0.1 M DDTßRNaseOUT Recombinant Ribonuclease InhibitorÝ40 U/ µLÞ Ý»40: 10777019ßInvitrogenÞ SuperScript Reverse TranscriptaseÝ200 U/ µLÞÝ»40: 18080093ß InvitrogenÞ$ 1.0 μL >Vß25S5 8×ß50S60 8×ß70S 15 8×d RT j$¸ k$*2T.R3¿gª" 2 Ó cDNA $uI "ßV 1 Ó cDNA $ÒT
(199) PCR $¸ Ðß® 1-1 ¶
(200) 3 ¨FL. 16.
(201) 'IT$ª" ß3' 5' ~¡Ör$×('N3JcÖ$ ¢ " 3 cDNA DL/!R=Ý7 1-1Þ$uI
(202) $`³ßl 1.5 μL FL'ITÝ10 µMÞ 25.0 μL KOD One PCR Master Mix -BlueÝ»40: KMM-201ßTOYOBOßßYÌÞ$ 21.0 µL ¨º>
(203) }F 1.0 μL ÒT cDNA $~>
(204) VßÂd PCR $¸ ß98S10 ×:ß62S5 ×%?TMR/ 68S25 ×Öj. "1'.N$ßÀ 35 P¡!¿
(205) . VÂjßPCR ¡%PT3NÔqyg!ßcDNA uI$µÄ
(206) ßTAEÝ40 mM Tris-acetate/1 mM EDTAÞÁÌ$ ª»
(207) 8(JEPI'>Ý0.5 µg/ mLÞ Ý»40: 161-0433ßBIO RADß%!M,ß,MD*N?%GÞm 1.2%%PT3NÝ»40: 31201193ßNippon GeneßßeÞ$ª
(208) PCR ¡$Ôqyg
(209) ß UV =LR3'N. AT7TÝATTOßßeÞ DNA BR>xp. $µÄ
(210) . 1-5. k$*2T.R3¿g. |fFicJÈ'$sy "ßRT-PCR !uI #V cDNA $ßk$*2T.R1T$ª¿g
(211) ß%PT3N '!7
(212) cDNA $ßNucleoSpin Gel and PCR Clean-upÝ»40: 740609ß Macherey-Nagelß>':ß<"TORÞ$ª
(213) »
(214) » cDNA $ Covaris S2 Focused-ultrasonicatorÝ»40: 500217ßCovarisß%!M,ßI 18"T49:GÞ$ªi@
(215) ßIon Plus Fragment Library KitÝ». 17.
(216) 40: 4471252ßThermo Fisher Scientificß%!M,ßI18"T49:GÞ "ßDNA L'ELMTª
(217) kßh. # DNA L'ELM. T$ßk$*2T.R1TIon Proton sequencerÝThermo Fisher ScientificÞ ¿g
(218) zß2T.R3ö
(219) Ion PI Hi-Q Sequencing 200 KitÝ»40: A26433ßThermo Fisher ScientificÞ$ß2T.R389F
(220) Ion PI Chip Kit v3Ý»40: A26770ßThermo Fisher ScientificÞ$ª ¿gh. #MT>tUÈ'U('N3JÈ'$. "ßCLC Genomics Workbench version 10.0 softwareÝCLC bioß<RI T.ß+TD3Þ$ª
(221)
(222) ÐßMDOR3È'
(223) ߥ£®('N 3 RV303 iJÈ'ÝGenBank %.492R0ßKY860613Þ$ª . 1-6. J~¡tUÈ'sy RNA ¤@. RT-PCR. VÂk$*2T.R3¿gsyJ 3' 5'~ ¡ÖrtUÈ'$¿¶ "ßJ RNA $¤@
(224) Vß%~¡Ör $m cDNA $ RT-PCR uI
(225) Ý7 1-2ÞJ RNA ¤@ ßRNA 3'~¡ 5'~¡$©F "x$ T4 RNA Ligase 1Ý»40: M0204SßNEBß%!M,ßI18"T49:GÞ$ª
(226) $`³ß 1.5 µL T4 RNA Ligase 1Ý10 U/ µLÞß2.0 µL 10 xReaction Bufferß4.0 µL 50% PEG 8000ß1.0 µL ATPÝ10 mMÞ 1.0 µL RNaseOUTÝ40 U/ µLÞ$ 5.5 µL RNase Wm¨º>" ·]
(227) jß *ÃJ RNA $ 5.0 µL ~>
(228) }F$ 2 z× 25d. 18.
(229) j h. #¤@ RNA $ßRNeasy MinElute Cleanup KitÝ»4. 0: 74204ßQiagenß>':ßCN<RÞ!»
(230) kßVÂ!¤@ # RNA $ÒT
(231) RT-PCR $¸
(232) Ð ßRT PCR $ Á¾
(233) 8" TE 5 ¸ E ¯ PrimeScript™ One Step RT-PCR Kit Ver.2Ý»40: RR055AßTakaraßß ºÞ$ª
(234) $`³ß2.0 µL PrimeScript 1 step Enzyme Mixß 25.0 µL 2 ×1step Bufferßl 2.0 µL FL'ITKoma-cirF1 KomacirR1Ý10 µMÞÝ® 1-2Þ$ß7 µL RNase Wm¨º>
(235) } F RNA $ 12.0 µL >VßÂd RT-PCR $¸ ß50S30 8×ß94S2 8×d RT $¸ß94S30 ×ß 58S30 ×ß72S1 8×j1'.N$ 30 P¡!¿ PCR $¸ kßh. # PCR ¡$ÒT
(236) Nested PCR $¸ßcDNA $. uI
(237) $`³ß12.5 μL KOD One PCR Master Mix -Blue-l 0.75 μL FL'ITKoma-cirF2 Koma-cirR2Ý10 µMÞÝ® 1-2Þ$ 6.0 μL ¨º~>
(238)
(239) ß }FV RT-PCR ¡ 5.0 μL $>ßÂd% PCR $¸ ß98S10 ×ß 55S5 ×ß68S5 ×j1'.N$ 30 P¡!¿
(240)
(241) ßÔqy g! cDNA uI$µÄ
(242) . 1-7. 1RT"2T.R3¿g. ('N3J~¡ÖrÈ'$sy "ßVÂ cDNA $1RF N
(243) ß1RT"2T.R3¿g$¸$`³ßBigDye. 19.
(244) Terminator v3.1 Cycle Sequencing KitÝ»40: 4337455ßApplied Biosystemsß %!M,ßI18"T49:GÞ$ªß2T.R3j$¸ Ðß FL'IT
(245) ßKoma-cirF2 Koma-cirR2Ý2 µMÞÝ® 1-2Þ$ª
(246)
(247) ß2T.R3j¡$ß3500 Series Genetic AnalyzerÝApplied BiosystemsÞ$ª¿g
(248) ßk$*2T.R3¿gV¿g!sy #|fFi cJÈ'ßNCBI GenBank <T7GT3ÍS$Ô #Ý%. 492R0ßLC553558Þ. 1-8. 8[¥<³¿g. |fFi¥£®('N3i8[¥<³Õ$Á " ß #. cJÈ'U8[¥j$
(249) ¿gßÍ+<. ³vF"À 55 i¥£®('N3i$ª
(250) Ý® 1-3Þ$`³ (66)8ÙU 8 ¥£ÝCosmopolitanßAfrica 3ß. ßTroupin. Arctic-RelatedßAfrica 2ßIndian subcontinentßAsianßRAK-SKßBatsÞ
(251) # #. ß]V('N3i$ÇT
(252) ß #. GenBankÝNCBIÞ. cJÈ'$. /h
(253) ]V 55 i|fFivÔÈ'$. "ß6D=()% MEGAXÝVer. 10.1Þ(35)m#"%FMT2R MUltiple Sequence Comparison by Log-ExpectationÝMUSCLEÞ$ª. vÔÈ'$ª
(254) ßGTR+G+I K<NUD!¥j$
(255) ßh. #¥jßl8o%"µ§ÝET=3=L. 9Fµ§Þ$ 1,000 PjR!³
(256) . 20.
(257) ß|fFi¥£ Arcitc-Related } "('N3i8[¥< ³Õ$VÂG !Á
(258) ß|fFicJÈ '¥£ Arctic-Related } "À 22 i Ý® 1-3ÞcJÈ'Uß 8[¥j$
(259) . 1-9. G¿g. |fFi RV303 iJlÍ+[G§$7 "ß MEGAXÝVer. 10.1Þ5 Distance !?"T$ªÍ+[×:§ÝpdistanceÞ$. 1-10. l('N3·Ç%. |fFil·Ç%. ÓÈ'p¼. ÓÈ'$ߥ£®('N3iGÈ'p. ¼
(260) $`³ß¬Voi RV303 iÝGenBank %.492R0: KY860613ÞßBV9901PJ iÝKC171645Þß1088 iÝAB645847ÞßRABV-Dog iÝLN879480Þ8yoi¬iiÝAB044824Þ ßCVS iÝLC325820Þ ß ERA iÝAB781935ÞÀ 7 i|fFil·Ç%. ÓÈ'Uß. MEGA X m#"%FMT2R MUSCLE $ªß # $
(261) . 21. vÔÈ'.
(262) © 1-1. ¥£®('N3|fFiJ. k$*2T.R1T$ªtUÈ'¿¶!ß|fFiJ RNA cÖ 11,927 tU" ¶ #Ý7 1-3Þh. #ctUÈ. 'Uß
(263) J$Á
(264) ©ßi NßPßMßG L Í +[+TFRSMT<&R/SDOTJÝORFÞ1' ß
(265) # # 1,353ß 894ß609ß1,575 6,384 tU ßi NßPßMßG L ·Çßl450ß297ß202ß524 2,127 %. ÓmU. #" uy #ßlÍ+[ ORF VßmRNA FÙf<$qÐeÔ;2/N Ý3'-UUGURRNGA-5'ßR A / G $ß N A / C / G / U $¶ Þ(15)ß. Ðe©2/NÝ3'-WCUUUUUUU-. 5'ßW A / U $¶ Þ(15)xp " ¶ #Ý® 1-4Þ . 1-2. |fFi*®³¥£®('N3i8[¥<³ÕÁ. |fFi¥£®('N3i8[¥<³Õ$y. ". ß|fFi*®³('N3iÀ 55 icJtUÈ'U ß8[¥j$
(266) Ý7 1-4Þ
(267) ©ßh. #¥jßTroupin. (66)VK # 8 ¥£ ÝCosmopolitanßAfrica 3ßArctic-Relatedß Africa 2ßIndian subcontinentßAsianßRAK-SK BatsÞ! #" µÄ # ¥jß|fFiߥ£ Arctic-Related } " ¶ #ß]V|fFi¥£Õß 100%ET=3=L9Fµ§!d #. 22.
(268) kß|fFi¥£ Arctic-Related } "('N3i8[¥ <³Õ$!´¼Á "ßicJÈ'ßG¥£} "À 22 iGÈ'U8[¥j$
(269) Ý7 1-5Þ
(270) ©ß Troupin ß. (66)VKG ߥ£ Arctic-Related 8Ù #('N3i 5 ¥£ÝArcticßAL1aßAL1bßAL2 AL3Þ8Ù. # #. ¥£ß|fFiß&RÖRßP2%eqr. e%%qr8Ñ #¬VoiÝ® 1-3Þ #"¥£ AL2 }
(271) ¢ß¥£ AL2 } "iß|fFieP2%7@ -« "¬Voi RV303 i¾ |fFi RV303 i¥<³ÕߥjV 100%ET=3=L9Fµ§ d #. 1-3. |fFi RV303 iJlÍ+[GÁ. kß|fFiß. i¾ " (y
(272) ¬Voi. RV303 icJÈ'$p¼ " ß%iÍ+<³¤$p¼SÁ
(273) 9ß%iJ1' p¼
(274) ©ßRV303 iJ ß11,927 tU #"|fFiJpß1 tU ¶ #Ý7 1-6Þ$`³ß|fFiJ 5'~¡%. ÓÚ0T>. Ör_¬ " 11,915 _ U ßRV303 iJ;
(275)
(276) UßlÍ+[ ORF tU\ÐeÔ;Ðe©2/NÈ'ß |fFi RV303 i×>cUÈ
(277) kß%icJtUÈ'G$Á
(278) ©ß #. 23. Jt.
(279) UÈ'G§ 98.2%Ý® 1-5Þ ßlÍ+[ ORF tUÈ' G§ß97.2~98.5%4l·Çuy%. ÓÈ'G§. $Á
(280) ß
(281) G§ 98.0% P ·Ç$×ßc ·Ç 99.0%]VÛG§$¶
(282) ß5 ('N3·Ç% ÓÈ'$p¼
(283) ©ß|fFi RV303 i×À 17 % UÅÄ. #Ý7 1-7Þ. 1-4. |fFi*®³('N3i N ·Ç%. kß|fFil·Ç%. ÓÈ'p¼. ÓÈ'$¬Voi RV303 iÈ'p. ¼
(284) Ý7 1-7Þ ßN ·ÇÈ'$p¼
(285) ©ß80 _ 1 % UÅÄ. Óm. Óm. #$`³ß|fFi N ·Ç 80 _ "%. ÓD)?N%L?RÝFÞ{
(286) ßRV303 i% RÝLÞ N ·Ç 80 _%. ÓP'2. ÓmUß('N3i×. "x$Á "ß|fFi*®³¬Voi8yoi N · Ç%. ÓÈ'$p¼
(287) Ý7 1-8Þ
(288) ©ßRV303 i$×ßÁ
(289) . c('N3iD)?N%L?RÝFÞmUx #" ¶ # ß®iÔ½" VK #" N ·Ç 273 _D) ?N%L?RÝFÞmU(43, 44, 60)ß394 _8P2RÝYÞmU(43, 44, 60) 395 _D)?N%L?RÝFÞmU(44, 60)ß #%. . ÓmU|fFi$mc('N3ix #Ý7 1-8Þ. 24.
(290) 1-5. |fFi*®³('N3i P ·Ç%. |fFi¬Voi RV303 i P ·Ç% X%. ÓmUÅÄ. 172 284 _%. ÓÈ'p¼. ÓÈ'ßÀ 6 . #Ý7 1-7Þ$`³ß60ß65ß82ß160ß. ÓmU|fFi RV303 i×. |fFi P ·Ç 60 _%3L RÓÝDÞ 172 _%N ? RÝRÞßRV303 i$×ßÁ
(291) c('N3i×x #Ý7 1-9Þ|fFi P ·Ç 284 _%3L RÝNÞßÁ
(292) ¦C\('N3ix #UßRV303 i$mm!C\ i4MRÝSÞ¬W
(293) ß65 160 _%. ÓmU. xßÁ
(294) ('N3iÝÀ 8 iÞßGÐ_ 4~5 ¹Ù% ÓmU #$ " µÄ #Uß|fFi P ·Ç 82 _%N ?RÝRÞmUßÁ
(295) ('N3ic/N7 RÝQÞmU¬W
(296) ß|fFi$mc('N3iß®iÔ½%. ÓmU. " 56ß58 66 _P'2RÝLÞmU(22, 60)ß81 _D)?N%L? RÝFÞmU(22, 60) 226 _%3L RÝNÞmU(22, 60)$
(297) " ¶ #Ý7 1-9Þ. 1-6. |fFi*®³('N3i M ·Ç%. |fFi¬Voi RV303 i M ·Ç% 2 X%. ÓmUÅÄ. M ·Ç 155 156 _%. ÓÈ'p¼. ÓÈ'×ßÀ. #Ý7 1-7Þ$`³ß|fFi. Óß
(298) # #=O+?RÝTÞ%. 25.
(299) N ?RÝRÞ{
(300) ßRV303 iG_¬%. Óß
(301) # #'. 6P'2RÝIÞM2RÝKÞ 2 %. ÓmUß. *®³('N3i%. ÓÈ'p¼
(302) ©ß|fFi M ·Ç . 155 _=O+?RÝTÞmUßÁ
(303) c('N3i'6P' 2RÝIÞmU¬W
(304) Ý7 1-10Þ Uß|fFi M ·Ç 156 _ %N ?RÝRÞmUßRV303 i$×ßÁ
(305) c('N3i× x # ß|fFi$mc('N3iß®iÔ½%. ÓmU. "ß95 _BMRÝVÞmU(24, 60)$
(306) " ¶ #Ý7 1-10Þ. 1-7. |fFi*®³('N3i G ·Ç%. |fFi¬Voi RV303 i G ·Ç% À 3 X%. ÓÈ'p¼. ÓÈ'p¼!ß. ÓmUÅµÄ #Ý7 1-7Þ $`³ß|fFi. G ·Ç-5 _Ý2/N
(307) F8>ÖrÞ ß187 _ 478 _%
(308) # #ßFPMRÝPÞß%L?RÝAÞ/N7 {
(309) ßGÐ_ RV303 i%. Ó. RÓÝEÞ. ÓmU
(310) # #ß4MRÝSÞß=O+?. RÝTÞ/M2RÝGÞß*®³('N3i%. Ó. È'p¼
(311) ©ß|fFi G ·Ç-5 _FPMRÝPÞmUßERA i% ÓÈ'Ä. #Ußm! 7 i RV303 iG. 4MRÝSÞmU$
(312) Ý7 1-11Þß|fFi G ·Ç 187 _ "%L?RÝAÞmUßix #. ßERA i. !8+?RÝMÞmUßERA i]Xi=O+?RÝTÞmU¬W
(313). 26.
(314) |fFi G ·Ç 478 _/N7. RÓÝEÞmUßÁ
(315) c. ('N3i/M2RÝGÞmU¬W
(316) ß|fFi$mc('N3iß®iÔ½%. ÓmU. 147 _M2RÝKÞmU(56)ß242 _%L?RÝAÞmU(27, 64)ß255 _ %3L RÓÝDÞmU(27, 64)ß268 _'6P'2RÝIÞmU(27, 64) 333 _%N ?RÝRÞmU(10, 63)$
(317) " ¶ #Ý7 111Þ kßG ·Ç N TÓ>´
(318) ßÓ>½%. ÓÈ'. Ý%3L R-X-4MR / =O+?R [X FPMR]Xc% \. Ó]Þ.
(319) _¬$Á
(320) Ý7 1-11Þ
(321) ©ß8yoi¬iiß. CVS i ERA i " G ·ÇVÓ>Ð_ 3 X!߬ ii ERA iß37ß247 319 _ßCVS i 37ß 204 319 __¬
(322)
(323) Uß|fFiPÁ
(324) ¬V oli " G ·ÇVÓ>Ð_\ 2 X!ß
(325) _¬ #i 37 319 _. 1-8. |fFi*®³('N3i L ·Ç%. |fFi¬Voi RV303 i L ·Ç%. ÓÈ'p¼. ÓÈ'×"%. ÓmUß118ß334ß597ß1,124 2,085 _À 5 XÖ # Ý7 1-7Þ #. 5%. ÓmUß*®³('N3i%. Ó. È'p¼
(326) ©ß|fFi L ·Ç 118 _ "=O+?RÝTÞmU ßRV303 i BV9901PJ $×ßÁ
(327) c('N3i. 27.
(328) x #Ý7 1-12ÞUß|fFi L ·Ç 334ß597ß1,124 2,085 _ "%L?RÝAÞßBMRÝVÞßD)?N%L?RÝFÞ %L?RÝAÞmUß #PÁ
(329) ('N3ic x #. ß
(330) # #BMRÝVÞß'6P'2RÝIÞßP'2RÝLÞ. =O+?RÝTÞmU¬W
(331) . 28.
(332) D ßk$*2T.R3¿g!ß|fFicJÈ'$s y
(333)
(334) ©ß('N3iJÝ7 1-3ß® 1-4Þߥ £®('N3iG " µÄ #(32)sy #ic JÈ'Ui¥<³È$uy
(335) ©ß|fFi¥£ Arctic-Related }
(336) ß P2%v{qr7@-. 8 Ñ #¬V oi. RV303 i¥<³¾ " ¶ #Ý7 1-4ß1-5Þ ß N Í+[tUÈ'U]x #Ä-¥<³¿g©(3, 37)U È
(337) cJÈ' N Í+[È'U¿g%®!G h. # ß|fFiJßLiu. VK
(338) G. ¨W(41)È $K¶5
(339) "]*ßArai ßN Í+ [È'U¥<³¿g©Uß|fFi"ß(k$¬Y Wx¾¿'³S½³xg$Ò ßYÑeqr 24
(340) uy
(341) "(3)h K¹. #. . # !ß uy. . #. |fFi¬Voi
(342) ©¡<³¤$
(343) "$Í+ <³!Ó. uy "ß|fFiß. i¾ " . (y
(344) ¬Voi RV303 icJÈ'$¢³p¼
(345)
(346) ©ß| fFi J 1' ß RV303 i Jp ß1 tU Ý7 1-6Þß%icJtUÈ'G§ß98.2%ßl·Ç%. ÓÈ. 'G§ 98.099.8% #ÚÛÝ® 1-5Þ ßÉ2| ßPÁ!ß%il·ÇÄ. 29. #%. ÓmUÅ.
(347) \ 17 " (y
(348) Ý7 1-7Þ |fFi 1940 * e'@. 8Ñ #ßKNK9=I(3 "Á¾*. ¿ #{
(349) (50)ßRV303 i 1980 *P2%v{qr7@. 8Ñ #"(37) ß8Ñ #*SqrSzX¹ß. .
(350) *"%iÙ
(351) Í+<³¤$
(352) . ßÚÉ2|¶Î" . ß
(353) # #('N3i. *$Ò ß%ilÇTSÝÆ³Â@ÞLÔ$
(354) # k u~ #. #$Ò ß¥£®('N3®i
(355) ©¡<³ ¤ß. l('N3·Ç¯LÔ$D %. Gy #"
(356) ß # l·Ç×Ä . ÓmU. kVUß|fFi RV303 i. #À 17 %. ÓÅÝ7 1-7Þl·Ç¯ß. ('N3¤D LÔD "
(357) |fFi RV303 i N ·Ç%. 1 X%. ÓÈ'$p¼
(358) ©ß80 _. ÓÅµÄ #Ý7 1-7ß1-8Þ ßPß%. ÓÈ'p¼ª¥£®('N3À 8 ißRV303 i N ·Ç 80 _P'2RÝLÞ$ß|fFi$m
(359) #]X('N3iD )?N%L?RÝFÞ$
(360) !ß Åß|fFi RV303 iC4 #:". #Uß('N3. ®iSÇ 3PίԽ" VK #" 273 _ß394 _ 395 _%. ÓmU(43, 44)ß|fFi RV303 i×>cx. #]*ßMasatani. (44)ßN ·ÇV #. 30. mUßzX¼.
(361) Å('N3 RNA 4R1T8[ßRetinoic acid-inducible gene IÝRIG-IÞ x@PÎÕ#
(362) ßI T'R7TD)PRÝIFNÞ©[;ß iÔ½" $VK
(363) |fFi # . ®. mU$
(364) . ßi N ·Ç RIG-I x@PÎx$
(365) ߮i?. #
(366) "E¯. #. |fFi RV303 i P ·Ç% ÓÈ'G§ 98.0Þß ·Çp¼
(367) ÕÁ
(368) Ý® 1-5ÞßP ·Ç vÀ 6 XÝ60 _ß65 _ß82 _ß160 _ß171 _ 284 _Þ% ÓÅµÄ #Ý7 1-7ß1-9Þ ]*ßDelmas. (9)ߥ£®('N. 3i$ml¹M91('N3J¿g!ß5 ¹Ù('N3·Ç & P ·Ç%. ÓÈ'G§ $¶
(369) ßP Í. +[ dN/dS ÝG¤¬W\{ "ÚG¤¬W\O6p§ÞÛ. $y
(370) ßP ·Çß('N3x¾Ù¯$¿
(371) . v\:$B "Ç$ $¶
(372) "|fFi. RV303 i P ·Ç× 6 %. ÓÅµÄ #§aß. P ·ÇÇÕ#
(373) ". #. Uß('N3®i IFN Í@xÔ½" VK #" P ·ÇV 5 %. ÓmUÝ56 _ß58 _ß66 _ß81 _. 226 _Þ(22)ß|fFi RV303 i%®>cx # Ý7 1-9Þ
(374) ß|fFi P ·Çß('N3®iÇ 3PίԽ¯$
(375) "uy #
(376)
(377) ß%i×Ä # 6 X%. ÓÅß60 _ß65 _ 82 _ß. 31.
(378) ¯³Ô½VÂ% ¾t
(379) " . ÓmU 3 Ý58 _ß66 _ 81 _Þ ßP ·Ç IFN Í@x-. LÔ$.
(380) "E¯1yß60 _ß65 _ 82 _%. Ó. IFN Í@xÕ#
(381) "#ß|fFi RV303 iß lzXg¡¹ " IFN ¥$f§³Í@ " P ·Ç¯ $Â@ ß
(382) # #zX¹Æ
(383) E¯ |fFi RV303 i M ·Ç% Ý155 _ 156 _Þ%. ÓÅÄ. #". ÓÈ'×ß2 X #Ý7 1-7ß1-10Þ #. ßM ·ÇVß('N3®iLÔ$D %. ÓmU
(384) . 95 _BMRÝVÞGy #"(24) BMRmUß·½¼Å %=T23[;Õ#
(385) ß©
(386) ß±5 "('N3rY ·½®i?#
(387) " ß95 _BMR$
(388) . #"(49)|fFi M ·Ç ßVÂ%=T23[;x. $
(389) ß('N3·½®iÔ½f<$
(390) " ¶5 # ß%. ÓÈ'p¼ªÀ 8 i('N3iß156 _M. 2RÝKÞ$
(391) RV303 i . ßGÐ_%. ÓÅßRV303 i:C4 #©"(i"Uß 155 _% Óß|fFi=O+?RÝTÞ$
(392) . =O+?RmU M ·Ç¯LÔ$#"Wy "ß|fFi©¡<³¤-. :@$.
(393) "E¯. 1y |fFi RV303 i G ·Ç%. 32. ÓÈ'p¼!ß-5 _ß.
(394) 187 _ 478 _%. ÓÅÄ #Ý7 1-7ß1-11ÞG ·Ç. N ~¡! 19 mUÝ-19~-1 _Þ2/N
(395) F8>ÖrßG · Ç "*ß N«¬5'i #"ß 19 mU('N3¤ [&m#" (8, 13, 32)
(396) ß|fFi%. ÓÈ' . -5 _FPMRÝPÞ('N3LÔ$D E¯ #"Uß¼ÅXÖr_¬ " 187 _Åߥ£®('N3kB `"?08R%48N0MRkB`uy©FÖrÝ189~214 mUÞ (39)¾. _¬
(397) 187 _% ÓmUßÁ
(398) . 8 iß|fFi%L?RÝAÞ$
(399)
(400) ß % L?RmUß|fFi G ·ÇkB`©F¯=LÔ$D
(401) " E¯. #"|fFi RV303 i" 478 _%. ßip¼ "ß|fFi6Uß/N7 478 _%. RÓÝEÞ$
(402) . ÓmUßG ·Ç¼Å5Ör_¬ " . ²$¹n
(403) " M ·ÇªLÔ$ "Uß('N3·½24Ô½%. Ó. ß°Ç(Ô.
(404) "E¯. ÓmU
(405) . #" 147 _. MRÝKÞ(56)|fFi RV303 i×>cx #ß ('N3·½®iÔ½" 242 _%L?RÝAÞß255 _%3L RÓÝDÞß268 _'6P'2RÝIÞ 333 _%N ?RÝRÞG x #(10, 27, 63, 64)]V!ß|fFi G ·Çß·½ 24·½®i½¯$
(406) "E¯Û #. #ßG ·ÇVxp " N TÓ>Ð_\߬Voi. 33.
(407) 8yoi×" VK #"(17, 74)$`³ß¬V oi G ·ÇV 1~2 Xß8yoi 3~4 XÓ>Ð_xp "Ý7 1-13Þ
(408) ß|fFi G ·ÇV%. ÓÈ'UßG·. Ç " N TÓ>Ð_\$Á
(409) ߬VoiG 2 X Ý37 _ 319 _Þ" $µÄ
(410) Ý7 1-11Þ]*ßYamada. . (73, 74)ßG ·ÇV 3 X N TÓ>Ð_$ "¬Vo:i ¿g$Ò ß Ó>Ð_u>('N3·½°3V j·½24$. $VK
(411) ¯ß¬Voi8y. oi·½°3·½24Å G ·ÇÓÙÅÕ# " $K¶5
(412) "
(413) ßU´³¬VoiG ßG · ÇV 2 XÓ>Ð_$ "|fFiß8yoi!·½ °3Û·½24$¶ E¯Û UßRNA M!LT5
(414) . #. #" L ·Ç%. ÓÈ'p¼. !ß|fFi RV303 i× 118 _ß334 _ß597 _ß1,124 _ 2,085 _À 5 X%. ÓÅµÄ #Ý7 1-7ß1-12Þ #. ß334 _ß597 _ß1,124 _ 2,085 _ 4 mUÅß # |fFi('N3i"% © . # #. ÓmU$:!h
(415) . :|fFi L ·Ç¯-. LÔ$D
(416) "E¯ kV^ß #. . #"ßL ·Ç8[¯Õ " :D ¯³LÔ$D " . 3Ó"
(417)
(418) ß('N3¼» " L ·Ç¯³Ô½$ "ß|fFi L ·Ç RNA M!LT5x$ß*®³¬Voi. 34.
(419) 8yoi L ·Çp¼ " ßB "Ô½Á×´ " # 1 ß|fFicJÈ'$sy
(420) VßGÈ'Ui ¥<³Èuyß. Í+<³¤Á$]x
(421)
(422) ©ß|. fFiP2%v{q7@- ¾ " (y
(423) . 8Ñ #¬Voi RV303 i. ß|fFi RV303 icJÈ'p. ¼!ß%iÍ+<³¤Ù
(424) " y | fFiC4 #l¹:i©¡<³¤"E¯1yßh. LÔ$D
(425). #Í+<³¯!ß|. fFi®i
(426) ©¡<³¤¬VoiÙ
(427) "E¯ Û. #. 35.
図
関連したドキュメント
The following result about dim X r−1 when p | r is stated without proof, as it follows from the more general Lemma 4.3 in Section 4..
Then it follows immediately from a suitable version of “Hensel’s Lemma” [cf., e.g., the argument of [4], Lemma 2.1] that S may be obtained, as the notation suggests, as the m A
0.1. Additive Galois modules and especially the ring of integers of local fields are considered from different viewpoints. Leopoldt [L] the ring of integers is studied as a module
[Mag3] , Painlev´ e-type differential equations for the recurrence coefficients of semi- classical orthogonal polynomials, J. Zaslavsky , Asymptotic expansions of ratios of
Failing to provide return transportation or pay for the cost of return transportation upon the end of employment, for an employee who was not a national of the country in which
[r]
Amount of Remuneration, etc. The Company does not pay to Directors who concurrently serve as Executive Officer the remuneration paid to Directors. Therefore, “Number of Persons”
関西学院大学手話言語研究センターの研究員をしております松岡と申します。よろ