水環境汚染物質の動態評価研究拠点の構築[平成１ ９年度報告書]

熊本大学学術リポジトリ

水環境汚染物質の動態評価研究拠点の構築[平成１ ９年度報告書]

著者 安部, 眞一, 北野, 健, 滝尾, 進, 古川, 憲治, 逸 見, 泰久, 木田, 建次, 森村, 茂, 湯, 岳琴, 川越 , 保徳, 嶋田, 純, 滝川, 清, 小池, 克明, 城, 昭 典, 河原, 正泰

発行年 2008‑03

URL http://hdl.handle.net/2298/7883

⎇ⓥߩ⋡⊛

ᧄ᜚ὐᒻᚑߢߪޔ࿾⃿ⷙᮨߢߩ⋭⾗Ḯ࡮ᜬ⛯࡮ᓴⅣ␠ળߩታ⃻ߦ߅޿ߡਛᩭߣߥࠆⅣ Ⴚ⑼ቇߩಽ㊁ߢ↹ᦼ⊛ߥ⽸₂ࠍߔࠆߚ߼ߦޔᾢᧄᄢቇߩ┙࿾᧦ઙߦ㈩ᘦߒޔ✕ᕆᕈߩ㜞

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࠾࠭ࡓ߇ਇ᣿ߩ߹߹∋ᑷ⁁ᘒߦ޽ࠆ᦭᣿࡮౎ઍᶏߩౣ↢ୃᓳ߳ߩ⽸₂ࠍᔨ㗡ߦ⟎߈ޔⅣ Ⴚࡎ࡞ࡕࡦߥߤߩⅣႺᳪᨴ‛⾰ߩ↢‛ࡕ࠾࠲࡝ࡦࠣ⑼ቇഃᚑޔᴪጯၞߦ߅ߌࠆᩕ㙃Ⴎ㒰

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᳓ߣᶏ᳓ߦ〙ࠆᴪጯၞ᳓ᓴⅣߩ⸘᷹࡮⸃ᨆ࡮⹏ଔᚻᴺߩ㜞ᐲൻߣᔕ↪ޔᓸ㊂ߢᄙ᭽ߥⅣ Ⴚᳪᨴ‛⾰ߩ㜞♖ᐲቯ㊂ᚻᴺߩ㐿⊒ߣᔕ↪ߥߤߩవዉ⊛⎇ⓥࠍផㅴߔࠆޕ߹ߚޔߎࠇࠄ ߩ⎇ⓥࠍㅢߓߡ✚ว⊛ߥ⎇ⓥ⢻ജߣ㜞ᐲߥᵢኤജࠍ᦭ߔࠆഃㅧᕈ⼾߆ߥੱ᧚ࠍ⢒ᚑߔ ࠆߣߣ߽ߦޔⅣႺ↥ᬺഃᚑߦ⽸₂ߔࠆޕ

౒ห⎇ⓥ⠪

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቟ㇱ⋿৻࡮⥄ὼ⑼ቇ⎇ⓥ⑼࡮ᢎ᝼

Ṛየ ㅴ࡮ᴪጯၞ࠮ࡦ࠲࡯࡮ᢎ᝼

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ᧁ↰ஜᰴ࡮⥄ὼ⑼ቇ⎇ⓥ⑼࡮ᢎ᝼

Ꮉ⿧଻ᓼ࡮⥄ὼ⑼ቇ⎇ⓥ⑼࡮ಎᢎ᝼

᫪᧛ ⨃࡮⥄ὼ⑼ቇ⎇ⓥ⑼࡮ಎᢎ᝼

㧔%㧕ᴪጯၞ᳓ᓴⅣߩ⹏ଔ

᎑↰ ⚐࡮⥄ὼ⑼ቇ⎇ⓥ⑼࡮ᢎ᝼

ṚᎹ ᷡ࡮ᴪጯၞ࠮ࡦ࠲࡯࡮ᢎ᝼

ዊᳰస᣿࡮⥄ὼ⑼ቇ⎇ⓥ⑼࡮ᢎ᝼

㧔&㧕ᓸ㊂ⅣႺᳪᨴ‛⾰ߩቯ㊂

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ᴡේᱜᵏ࡮⥄ὼ⑼ቇ⎇ⓥ⑼࡮ᢎ᝼

̪ᐔᚑ㧝㧤ᐕ㧠᦬ᡷ⚵ߦࠃࠅޔᣥℂቇㇱޔᎿቇㇱᚲዻߩᢎຬߪᄢቇ㒮⥄ὼ⑼ቇ⎇ⓥ⑼ᚲ ዻߦߥࠅ߹ߒߚޕ

⋡⊛ߦኻߔࠆㅴ᝞⁁ᴫ

ਅ⸥ߦ␜ߔࠃ߁ߦޔฦࠣ࡞࡯ࡊߢߩㅴ᝞⁁ᴫߪ㗼⪺ߢ޽ࠅޔ੐ᬺផㅴᜂᒰ⠪

⋧੕ߩ᦭ᯏ⊛ㅪ៤߽ᷓ߹ࠅߟߟ޽ࠆߩߢޔ㗅⺞ߦ⊒ዷߒߡ޿ࠆߣ⠨߃ࠄࠇࠆޕ

㧔A㧕ⅣႺᳪᨴ‛⾰ߩ↢‛ࡕ࠾࠲࡝ࡦࠣ

࡮੹ᐕᐲߪޔ㜞᳓᷷ߦࠃࠆࡅ࡜ࡔߩ㓶ൻࠍ⺃ዉߒߡ޿ࠆน⢻ᕈ߇޽ࠆಽᴲᕈ࿃ሶ䋨ࠦ࡞

࠴࠱࡞䋩ߩหቯߦᚑഞߒߚޕ߹ߚޔࠗࡕ࡝♖Ꮍߩ♖ේ⚦⢩ౝߢ⊒⃻ߔࠆRNA⚿ว࠲ࡦࡄ

ࠢ⾰߇ޔ╙㧣਎ઍ♖ේ⚦⢩ߢߩߺ⊒⃻ߒޔࡊࡠ࡜ࠢ࠴ࡦߦࠃߞߡᶖᄬߔࠆߎߣޔ᛫ࠕࡐ

࠻࡯ࠪࠬᵴᕈࠍ᦭ߒߡ޿ࠆߎߣޔࠕࡐ࠻࡯ࠪࠬߩଦㅴߦ㑐ਈߔࠆPdcd4ߣ⚿วߔࠆߎߣޔ ࠍ᣿ࠄ߆ߦߒߚߩߢޔࡊࡠ࡜ࠢ࠴ࡦߦࠃࠆࠕࡐ࠻࡯ࠪࠬ⺃⿠ᯏ᭴ߦߟ޿ߡℂ⸃߇ᷓ߹ߞ ߚޕ߹ߚޔࠗࡕ࡝↢ᱺ⚦⢩ߩಽൻߦኻߔࠆࡆࠬࡈࠚࡁ࡯࡞ A ߩᓇ㗀ߦߟ޿ߡၭ㙃♽ࠍ

↪޿ߡ⺞ߴߚޕߎࠇࠄߩ⚿ᨐ߆ࠄޔ⋡ᮡߪචಽ㆐ᚑߢ߈ߚߣ⠨߃ࠄࠇࠆޕ

࡮;EH ߩࠕࡦࡕ࠾ࠕߦࠃࠆ⊒⃻⺃ዉ߇⪲⁁૕ߛߌߢߥߊ♻⁁૕ߦ߅޿ߡ߽⏕⹺ߢ߈ߚޕ

߹ߚ㧘㌃ࠬ࠻࡟ࠬߦኻߒߡ߽♻⁁૕ߪ⪲⁁૕ߣห᭽ߦ⦡⺞ᄌൻࠍ␜ߔߩߦኻߒ㧘⓸⚛ᰳ

ਲࠬ࠻࡟ࠬߦኻߒߡߪ♻⁁૕ߪ⠴ᕈࠍ߽ߟߎߣ߇᣿ࠄ߆ߦߥߞߚޕߎߩࠃ߁ߦ㧘♻⁁૕

ߢߪ⪲⁁૕ߣߪ⇣ߥࠆࠬ࠻࡟ࠬᔕ╵ࠍ߽ߟߎߣ߇᣿ࠄ߆ߦߥࠅ㧘♻⁁૕ߢߩࠬ࠻࡟ࠬᔕ

╵․ᕈࠍ᣿ࠄ߆ߦߔࠆߣ޿߁ᧄᐕᐲߩ⋡ᮡߪචಽ㆐ᚑߐࠇߚߣ⠨߃ࠄࠇࠆޕ

࡮ࡂࡑࠣ࡝ߩ⒩⽴ߩ⌕ᐩ႐ᚲߣߒߡ㧘⍾࿾㧘․ߦᴡᎹౝߩ⍾࿾ߩ㊀ⷐᕈ߇᣿ࠄ߆ߦߥߞ ߚ㧚ടᏓ㉿ߢߪᄙߊߩ⒩⽴߇ᴡᎹౝߦ⌕ᐩߒ㧘ᚑ㐳ߣ౒ߦᶏၞߦ⒖േߒߚ㧚⊕Ꮉᴡญߢ

߽㧘ᄐቄߩᄢ㔎ߩᓟߦᴡᎹౝߩࡂࡑࠣ࡝߇ᷫዋߒ㧘ㅒߦᶏၞߩࡂࡑࠣ࡝߇Ⴧടߒߚ߇㧘 ߎࠇߪ㧘ᴡᎹ߆ࠄᶏၞ߳ߩࡂࡑࠣ࡝ߩฃേ⊛ߥ⒖േߢ޽ࠆߣ⠨߃ࠄࠇࠆ㧚ߎࠇࠄߩ੐ታ ߪ㧘⍾࿾㧘․ߦᴡᎹౝߩ⍾࿾ߩⅣႺߩᖡൻ߇ࡂࡑࠣ࡝⾗ḮߩუṌߦ⋥⚿ߔࠆߎߣࠍ␜ໂ ߒߡ޿ࠆ㧚ࡂࡑࠣ࡝ỗᷫߩේ࿃ߣߒߡ㧘ᴡᎹౝߩ⍾೑ណข߿⍾㒐࠳ࡓᑪ⸳ߦࠃࠆᵹਅ⍾

㊂ߩᷫዋ߇ᜰ៰ߐࠇߡ޿ࠆ߇㧘ᧄ⎇ⓥߩ⚿ᨐ߽ߘࠇࠍᡰᜬߒߡ޿ࠆ㧚

㧔B㧕ⅣႺᳪᨴ‛⾰ߩࡃࠗࠝ࡮࡟ࡔ࠺ࠖࠛ࡯࡚ࠪࡦ

࡮ᐔᚑ ᐕᐲߩ⎇ⓥᚑᨐࠍฃߌߡޔ੹ᐕᐲߪ #PCOOQZ ෻ᔕߩታឃ᳓߳ߩㆡ↪ࠍⷞ㊁ߦޔ ታឃ᳓ࠍಣℂኻ⽎ߦ⎇ⓥࠍㅴ߼ߚޕታឃ᳓ߦߪޔߎࠇ߹ߢ૶↪ߒߡ߈ߚวᚑήᯏឃ᳓ߣ

㆑ߞߡޔ᦭ᯏ‛߿ 55 ߣ޿ߞߚ #PCOOQZ ෻ᔕࠍ㒖ኂߔࠆᚑಽ߇฽᦭ߐࠇޔታឃ᳓ಣℂߢ

໧㗴ߣߥࠆޕߘߎߢޔ55 ฽᦭㊂ߩᄙ޿ࡃࠗࠝࠟࠬࡊ࡜ࡦ࠻⣕㔌ᶧࠍಣℂኻ⽎ߦޔ2') ߢ ൮᜝࿕ቯൻߒߚ⎣ൻࡍ࡟࠶࠻ࠍ↪޿ߚㇱಽ੝⎣㉄ൻಣℂࠍⴕ޿ޔࡃࠗࠝࠟࠬࡊ࡜ࡦ࠻⣕

㔌ᶧਛߩࠕࡦࡕ࠾ࠕߩ⚂ඨ㊂ࠍ੝⎣㉄ߦ㉄ൻߔࠆㇱಽ੝⎣㉄ൻಣℂࠍޔMI0OFC[

ߩ⽶⩄ၞߢᵹ౉᳓ߩ᦭ᯏ‛ޔ55 Ớᐲߩᓇ㗀ࠍฃߌࠆߎߣߥߊ቟ቯߒߡⴕ߁ߎߣ߇ߢ߈ ߚޕ⛯ߊ #PCOOQZ ಣℂࠍޔหߓߊ 2') ߢ൮᜝࿕ቯൻߒߚ #PCOOQZ ࡍ࡟࠶࠻ࠍ↪޿ߡⴕߞ ߚޕᒰೋߪޔㇱಽ੝⎣㉄ൻಣℂ᳓ࠍᏗ㉼ߒߡ⹜㛎ߒߚ߇ޔᦨ⚳⊛ߦᏗ㉼ߔࠆߎߣߥߊㇱ ಽ ੝ ⎣ ㉄ ൻ ಣ ℂ ᳓ ࠍ ߘ ߩ ߹ ߹ #PCOOQZ ࡍ ࡟ ࠶ ࠻ ࠍ ↪ ޿ ߚ ࡝ ࠕ ࠢ ࠲ ߦ ଏ ⛎ ߒ ޔ

MI0OFC[ ߣ޿߁㜞޿⓸⚛⽶⩄㊂ߢ #PCOOQZ ಣℂ߇น⢻ߢ޽ࠆߎߣࠍ⹺߼ߚޕᧄታ 㛎ߢ↪޿ߚ #PCOOQZ ࡍ࡟࠶࠻ߪ 2') ♽ߩࠥ࡞ߦ #PCOOQZ ⩶߇൮᜝࿕ቯߐࠇߡ޿ࠆߎߣ߆ ࠄޔ᦭ᯏ‛߿ 55 ߩᓇ㗀ࠍ #PCOOQZ ⩶߇ฃߌ㔍ߊߥߞߡ޿ࠆޕ੍ᗐㅢࠅߦޔ#PCOOQZ ࡍ

࡟࠶࠻ߪ᦭ᯏ‛ޔ55 ߩᓇ㗀ࠍฃߌࠆߎߣߥߊᯏ⢻ߔࠆߎߣࠍታឃ᳓ಣℂߢ⹺߼ߚޕ ㇺᏒਅ᳓ಣℂ႐ߩህ᳇ᕈᶖൻ⣕㔌ᶧࠍಣℂኻ⽎ߦޔំേᐥߦࠃࠆㇱಽ੝⎣㉄ൻಣℂࠍⴕ

޿ޔMI0OFC[ ߩ㜞޿⽶⩄ၞߢޔ#PCOOQZ ߦㆡߒߚㇱಽ੝⎣㉄ൻಣℂࠍ㆐ᚑߢ߈ߚޕ

⛯ߊ $: ࠍᜂ૕ߣߔࠆ #PCOOQZ ಣℂߢ߽᦭ᯏ‛߿ 55 ߩᓇ㗀ࠍฃߌࠆߎߣߥߊ #PCOOQZ ෻ ᔕ߇⿠ߎࠆߎߣࠍ⹺߼ߚޕ

࡮࿾⃿߇ᛴ߃ࠆᄢ߈ߥⅣႺ໧㗴ߩ৻ߟߢ޽ࠆⅣႺࡎ࡞ࡕࡦ᭽‛⾰ߩ໧㗴ߦ㑐ߒߡޔวᚑ ൻว‛ߛߌߢߥߊᄤὼࡎ࡞ࡕࡦ߇ᐢߊಽᏓߔࠆߎߣ߆ࠄޔህ᳇߅ࠃ߮ᅢ᳇⊛↢‛ಣℂߣ

ࠝ࠱ࡦ㉄ൻࠍ⚵ߺวࠊߖߚಣℂࡊࡠ࠮ࠬࠍ⎇ⓥ࡮㐿⊒ߒߚޕ߹ߚޔ㤥ᄢ⼺ᾚ᳝㉯ㅧ㈶߆ ࠄන㔌ߐࠇߚCompound-6ߪ㤥ᄢ⼺ᾚ᳝ߦ․⇣⊛ߢߪߥߊޔ☨ޔ⦱߿㤈὾㈪☺߅ࠃ߮὾

㈪☺߆ࠄ⵾ㅧߒߚ㉯ㅧ㈶ߦ߽฽߹ࠇߡ޿ࠆߎߣ߇ࠊ߆ߞߚޕ὾㈪☺ߪ὾㈪ߩ⵾ㅧㆊ⒟ߢ ᄢ㊂ߦ೽↢ߔࠆ߽ߩߢ޽ࠅޔએ೨ߪᶏᵗᛩ᫈߿὾ළߦࠃߞߡಣಽߐࠇߡ޿ߚ߇ޔ὾㈪☺

߆ࠄኈᤃߦ᦭↪‛⾰ࠍ᛽಴ߔࠆᛛⴚ߇⏕┙ߐࠇࠇ߫ޔఝࠇߚ࡝ࠨࠗࠢ࡞ᣇᴺߦߥࠆߣ⠨

߃ࠆޕᄢ⼺ᾚ᳝㉯ㅧ㈶߿ฦ⒳὾㈪☺ਛߩCompound-6฽᦭㊂ࠍ⺞ߴߚߣߎࠈޔߘࠇࠄ1 kg ਛߦ 5㨪15 㨙㨓⒟ᐲߒ߆฽߹ࠇߡ޿ߥ߆ߞߚޕCompound-6 ߪ⚦⢩ၭ㙃♽ߦ߅޿ߡޔ U937⚦⢩ߦኻߒߡ40-80 ȝg/mlߩỚᐲߢࠕࡐ࠻࡯ࠪࠬࠍ⺃ዉߔࠆ߇ޔCompound-6߇↢

૕ߦ߅޿ߡታ㓙ߦߤߩߊࠄ޿ߩല₸ߢዊ⣺߆ࠄๆ෼ߐࠇޔࠟࡦ⚦⢩ߦኻߒߡߤߩࠃ߁ߦ

૞↪ߔࠆ߆ޔ߹ߚޔ⵾ㅧߒߚ㤥ᄢ⼺ᾚ᳝㉯ㅧ㈶ߩ in vivo ߢߩታ㛎⚿ᨐߦߤߩߊࠄ޿ነ ਈߒߡ޿ࠆߩ߆ߪޔᧄ⎇ⓥߩߺߢߪోߊਇ᣿ߢ޽ࠆޕ߹ߚޔ਎⇇ߩ㘩ຠᯏ⢻ᕈ⎇ⓥߦ߅

޿ߡࡅ࠻⥃ᐥ⹜㛎ޔേ‛⹜㛎߅ࠃ߮⚦⢩ၭ㙃⹜㛎ࠍ᣿⏕ߦ㑐ㅪߠߌߡ⺑᣿ߒߚႎ๔ߪߥ

޿ߚ߼ޔ‛⾰ߩᕈ⾰ߣ៨ข㊂ߦኻߔࠆዊ⣺ౝߢߩๆ෼ߦ߅ߌࠆ㑐ㅪᕈߩ⸃᣿߇ᦸ߹ࠇࠆޕ

࡮* ᐕᐲ߹ߢߩ⎇ⓥߢߪ↢ಽ⸃ᕈࡊ࡜ࠬ࠴࠶ࠢ 2*$ ࠍන৻὇⚛Ḯߣߒߡ↢⢒น⢻ߢ ߆ߟ⎣㉄ࠍ⓸⚛ࠟࠬߦ߹ߢㆶర㒰෰น⢻ߥ 2*$ ⾗ൻᕈ⣕⓸⚦⩶ -( ᩣ߇ಽ㔌ߐࠇޔㅪ⛯

⎣㉄㒰෰ᛛⴚ⏕┙ߦะߌߚඨㅪ⛯ታ㛎㧔ㅙᰴ࿁ಽၭ㙃ታ㛎㧕߇ᓧࠄࠇߚޕᧄᐕᐲߪޔߐ ࠄߦ㐳ᦼߦᷰࠆㅙᰴ࿁ಽታ㛎ࠍⴕߞߡޔ-( ᩣߦࠃࠆ㜞Ớᐲߩ⎣㉄ᕈ⓸⚛㒰෰⢻ࠍ⏕⹺

ߒޔᦨ⚳⊛ߦޔ↢ಽ⸃ᕈࡊ࡜ࠬ࠴࠶ࠢࠍ὇⚛Ḯߣߔࠆㅪ⛯⣕⓸࡝ࠕࠢ࠲ࠍ᭴▽ߒޔ⦟ᅢ ߥ⚿ᨐߣߘߩ᦭↪ᕈࠍ᣿ࠄ߆ߦߔࠆߎߣ߇ߢ߈ߚޕ⃻࿷ޔߎࠇࠄߩ⎇ⓥ⚿ᨐࠍ⺰ᢥߦ߹

ߣ߼ߡ߅ࠅޔᧄᐕᐲߩᚑᨐߪචಽ㆐ᚑߒߚ߽ߩߣ⠨߃ࠆޕ

㧔C㧕ᴪጯၞߩ᳓ⅣႺߩ⹏ଔ

࡮ࠊ߇࿖ߦ߅ߌࠆ %(%㨟᷹ቯߦߪ㧘ੱὑ⊛ߥᳪᨴ߇ᱴߤሽ࿷ߒߡ޿ߥ޿ࠃ߁ߥ࿾ၞߦ߅ ߌࠆᄢ᳇Ớᐲߩ㐳ᦼ᷹ⷰߪߥߊ㧘ਇ⍮Ἣᵹၞߦ߅ߌࠆᄢ᳇Ớᐲ᷹ቯ୯߿⑔ጟᏒߦ߅ߌࠆ

᷹ⷰ⚿ᨐ߆ࠄޔṛ⇐ᤨ㑆ផቯߦߪ ᐕ㨪 ᐕ⒟ᐲߩ⺋Ꮕߪ࿷ࠅ߁ࠆ߽ߩߩޔർඨ⃿ᐔ ဋ୯ࠍᚒ߇࿖ߢߩ %(%㨟౉ജ୯ߣߒߡలಽ೑↪ߔࠆߎߣ߇ߢ߈ࠆ߽ߩߣ್ᢿߐࠇߚޕ

࡮᦭᣿࡮౎ઍᶏߩⅣႺౣ↢ߣ㒐ἴߦ߆߆ࠊࠆ✚ว⎇ⓥࠍផㅴߔࠆߦᒰߚߞߡޔቇⴚ⎇ⓥ

ߪ߽ߣࠃࠅޔ᡽╷╷ቯ߳ߩ⋥ធᜰዉ࡮ឭ⸒ޔ࿾ၞ␠ળ૞ࠅ╬ޘޔⓍᭂ⊛ߦផㅴ࡮ዷ㐿ࠍ ⴕ޿ᄙᄢߩታ❣ߣ⽸₂ࠍᨐߚߒߡ޿ࠆޕ

࡮ ߟߩࠨࡉ࠹࡯ࡑߣ߽ߦᐔᚑ ᐕᐲߩ໧㗴ὐߦኻಣߒ㧘ᣂߚߥ⸃ᨆᚻᴺ߿᷹ቯᣇᴺ ࠍ⷗಴ߒߚߣߣ߽ߦ㧘ᅷᒰߥ⸃ᨆ⚿ᨐ߿᷹ቯ࠺࡯࠲ࠍჇ߿ߔߎߣ߇ߢ߈ߚޕ೨ᐕᐲߩᶏ ᐩ㔚᳇តᩏߦട߃ߡᴪጯၞ㔚᳇តᩏߣ޿߁ᣂߚߥ⸘᷹ᣇᴺߦߟ޿ߡ߽᦭᣿ᶏߦㆡ↪ߢ ߈㧘೨ᐕᐲએ਄ߦ㊀ⷐߥ⍮⷗߇ᓧࠄࠇߚޕߎࠇࠄߩⷰὐ߆ࠄߪ㧘೨ㅀߩᐔᚑ ᐕᐲߩ

⋡⊛࡮⋡ᮡߦ㑐ߒߡ߶߷㆐ᚑߢ߈ߚߣ⠨߃ࠆޕ੹ᓟߐࠄߦ࡝ࡕ࡯࠻࠮ࡦࠪࡦࠣ㧘ᤨⓨ㑆 ಽᏓࡕ࠺࡝ࡦࠣ㧘ᔕ↪࿾⃿‛ℂߦࠃࠆ᳓ၞ࡮࿾࿤ⅣႺߩ⎇ⓥࠍ⊒ዷߐߖߚ޿

㧔D㧕ᓸ㊂ⅣႺᳪᨴ‛⾰ߩቯ㊂

࡮ᐔᚑᐕᐲߪޔᤓᐕᐲ㐿⊒ߒߚࠬ࡞ࡎࡦ㉄ߣࠕࡒࡁࡔ࠴࡞ࡎࠬࡎࡦ㉄ࠍ᦭ߔࠆࠠ࡟࡯

࠻❫⛽(025߇%W++ߥߤߩ㧞ଔࠗࠝࡦߦߟ޿ߡߪޔⓨ㑆ㅦᐲJߣ޿߁㜞ㅦㅢᶧ

᧦ઙਅߢ߽⎕ㆊኈ㊂߇⚂OGSIߦ߽෸߱ߎߣࠍ᣿ࠄ߆ߦߢ߈ߚޕ✢ㅦᐲߪEOU ߢ޽ࠅޔߎࠇߪṁᶧ߇㧝⑽㑆ߦ❫⛽ᐥߩ㐳ߐߩ୚ㅴ߻ᵹㅦߢ޽ࠆޕᓥߞߡޔߎߩ❫

⛽ߪଔ㊄ዻࠗࠝࡦߩ㜞ㅦỚ❗߳ߩᔕ↪߇᦭ᦸߢ޽ࠆޕ(025ߩ(G+++ߩๆ⌕ᕈ߽⹏ଔߒ ߚ߇ޔ(025ߪ(G+++ࠍㄦㅦߦๆ⌕ߒߥ߆ߞߚ߶߆ޔ+P+++)C+++ߩๆ⌕ᕈ߽ࠗࡒ ࡁੑ㈶㉄ߣࠬ࡞ࡎࡦ㉄ၮࠍ᦭ߔࠆ❫⛽(%5+5ࠃࠅ߽ๆ⌕ㅦᐲ߇ㆃ޿௑ะ߇⹺߼ࠄࠇߚޕ ଔߩ㊄ዻࠗࠝࡦߦߟ޿ߡߪ(%5+5߇ㄦㅦߥๆ⌕ㅦᐲࠍ᦭ߔࠆߎߣ߇್ߞߚޕ⎣㉄ࠗࠝࡦ ๆ⌕↪ߩ❫⛽(6'#ߥࠄ߮ߦ($&#ߦߟ޿ߡߪޔᧄᐕᐲ߆ࠄ⎇ⓥࠍ㐿ᆎߒߚ߇ⓨ㑆ㅦᐲ J߹ߢޔ⎕ㆊᦛ✢ᒻ⁁߇ㅢᶧㅦᐲߦଐሽߒߥ޿⚿ᨐ߇ᓧࠄࠇߚߩߢㅦᐲ㕙ߢߪḩ⿷ߥᚑ ᨐࠍᓧࠆߎߣ߇ߢ߈ߚޕ

࡮੹ᐕᐲߪޔ㌃⵾㍰ߢ⊒↢ߔࠆࠬ࡜ࠣ߆ࠄߩ㊀㊄ዻߩṁ಴ᕈޔ․ߦࡅ⚛ߩṁ಴ߦߟ޿ߡ ߩ⍮⷗ࠍᓧࠆߎߣ߇ߢ߈ߚߚ߼ޔ⋡⊛ߪචಽߦ㆐ᚑߐࠇߚ߽ߩߣ⠨߃ࠄࠇࠆޕ

ᐔᚑ㧝㧥ᐕᐲඳ჻ᓟᦼ⺖⒟ቇ↢ᢙ

ㅴቇ↢ ⇐ቇ↢ ␠ળੱ

቟ㇱ⋿৻

Ἇየ ㅴ ㅺ⷗ᵏਭ ฎᎹᙗᴦ ᧁ↰ஜᰴ

Ꮉ⿧଻ᓼ

᎑↰ ⚐ ṚᎹ ᷡ ዊᳰస᣿

ၔ ᤘౖ

ᴡේᱜᵏ

㧟 㧜 㧞 㧜 㧜 㧞 㧟 㧜 㧜 㧜 㧜

㧟 㧝 㧜 㧠 㧞 㧜 㧝 㧜 㧠 㧝 㧜

㧣 㧜 㧝 㧝 㧝 㧜 㧜 㧠 㧞 㧞 㧝

⸘ 㧝㧜 㧝㧢 㧝㧥

ᐔᚑ ᐕᐲߩ⎇ⓥ⚻⾌૶↪ታ❣ႎ๔ᦠ

᜚ὐ㧮⎇ⓥ⚻⾌㧔 ਁ౞㧕

⎇ⓥ⚻⾌ౝ⸶ ㊄㗵㧔ජ౞㧕 Ⓧ ▚ ౝ ⸶ 㧔ජ౞㧕

⸳஻஻ຠ⾌

ੱ ઙ ⾌

⑳⾌⇐ቇ↢⵬ഥ㧔ୃ჻ 㧘ඳ჻ 㧦 㧔㧰ቇ↢㧬㧘㧹ቇ↢"㧕 ࡎ࡯ࡓࡍ࡯ࠫ૞ᚑ⵬ഥ㧔㧬㧕

౒ห⎇ⓥ

ࠣ࡞࡯ࡊ㑆౒ห⎇ⓥ⾌ᡰេ⾌

⎇ⓥ⺖㗴㧦ޟᲧᛶ᛫ᴺߦࠃࠆᴪጯၞᢿጀਛߩႮ

᷆Ⴚ⇇ಽᏓߩ㜞♖ᐲࠗࡔ࡯ࠫࡦࠣޠ㧘ޟႮᕈḨ

࿾ߩౣ↢ࠍ⋡⊛ߣߒߚႮᕈᬀ‛ߩ❥ᱺ↢ᘒߣ୘

૕⟲㑆ߩㆮવ⊛㘃✼㑐ଥߩ⸃ᨆޠ

ᣏ ⾌ ࡊࡠࠫࠚࠢ࠻࠯ࡒ⻠Ꮷᣏ⾌

੐ᬺផㅴ⾌

࿖㓙ࠪࡦࡐࠫ࠙ࡓ㐿௅⵬ഥ⾌

ߘ ߩ ઁ

⎇ⓥᜰዉ⵬ഥ⾌

㒮↢ߩ࿖㓙ળ⼏⊒⴫⵬ഥ㧔"㧖㧕 ᶧ᥏ࡊࡠࠫࠚࠢ࠲࡯㧔㧝㧕

ႎ๔ᦠශ೚ઍ㧔*㧝㧥ᐕᐲ 㧘ᦨ⚳ႎ๔ 㧕

ว ⸘

ߎߩ᜚ὐ⎇ⓥߦ㑐ㅪߒߚᄖㇱ⾗㊄ߩ₪ᓧ⁁ᴫ

㧔⑼ቇ⎇ⓥ⾌⵬ഥ㊄ޔઁ⋭ᐡ࡮⎇ⓥഥᚑᴺੱ࡮᳃㑆ડᬺ߆ࠄߩ⎇ⓥ⾌╬㧕

቟ㇱ ⋿৻

࡮ၮ⋚⎇ⓥ䋨B䋩, 2006-2008 ᐕ, 550 ਁ౞䋨ᐔᚑ

ᐕᐲಽ䋩, ♖ሶಽൻߦ߅ߌࠆᣂⷙ ࡄ࡜ࠢ࡝ࡦ࿃ሶ࠾ࡘ࡯࡟ࠡࡘ࡝ࡦߩᯏ⢻

࡮ℂ⎇ߣߩ౒ห⎇ⓥ⾌, 2007 ᐕᐲ

ਁ౞ޔ↢ᱺ∔ᖚࡕ࠺࡞േ‛ߩ㐿⊒

ർ㊁ ஜ

࡮․ቯ㗔ၞ⎇ⓥ䋨౏൐⎇ⓥ䋩

ᐕ

ਁ౞䋨ᐔᚑ

ᐕᐲಽ䋩

᷷ᐲଐሽᕈ ᕈ᳿ቯߩಽሶࡔࠞ࠾࠭ࡓ

࡮⧯ᚻ⎇ⓥ

ᐕ

ਁ౞䋨ᐔᚑ

ᐕᐲಽ䋩

ࡔ࠳ࠞᕈಽൻߦ߅ߌࠆ⚦⢩Ⴧ ᱺ࿃ሶߩᓎഀ

Ṛየ ㅴ

࡮ၮ⋚⎇ⓥ㧯 ਁ౞ᐔᚑᐕᐲಽ࡟࠻ࡠ࠻࡜ࡦࠬࡐ࠱ࡦߦࠃࠆࠬࠨࡆࡁ

࡝ಽሶ⢒⒳ᴺߩ㐿⊒

ㅺ⷗ ᵏਭ

࡮⑼ቇ⎇ⓥ⾌⵬ഥ㊄ၮ⋚⎇ⓥ 䋨

䋩㧔ઍ⴫㧕

ᐕᐲ

ਁ౞

ࡂࡑࠣ࡝ߩ⾗Ḯ

࿁ᓳߣᜬ⛯⊛೑↪ߩߚ߼ߩ⎇ⓥ

࡮᡽╷ഃㅧ⎇ⓥ࠮ࡦ࠲࡯࡮ࡊࡠࠫࠚࠢ࠻⎇ⓥ㧔ಽᜂ㧕

ᐕ

ਁ౞

᦭᣿ᶏ࡮

౎ઍᶏߩ↢‛↢ᕷⅣႺߩ⹏ଔ࡮଻ో࡮ౣ↢

ᧁ↰ ஜᰴ

࡮ฃ⸤⎇ⓥ㧔0'&1㧕ޔᐕޔ౞ޔࡃࠗࠝࡑࠬࠛࡀ࡞ࠡ࡯㜞ല₸ォ

឵ᛛⴚ㐿⊒㧛ࡃࠗࠝࡑࠬࠛࡀ࡞ࠡ࡯వዉ⎇ⓥ㐿⊒㧛ㇺᏒဳࡃࠗࠝࡑࠬ⾗Ḯ߆ ࠄߩ㜞ല₸ੑᲑ⊒㉂ߦࠃࠆΆᢱ↪ࠛ࠲ࡁ࡯࡞⵾ㅧᛛⴚߩ㐿⊒

࡮ฃ⸤⎇ⓥ㧔ፏၔᄢቇ㧕ޔᐕޔ౞ޔᐔᚑᐕᐲޟᣂࠛࡀ࡞ࠡ࡯ᛛ ⴚ㐿⊒ࡊࡠࠣ࡜ࡓ㧛ᣂࠛࡀ࡞ࠡ࡯ᛛⴚ⎇ⓥ㐿⊒㧛ᣂࠛࡀ࡞ࠡ࡯ࡌࡦ࠴ࡖ࡯ᛛ ⴚ㕟ᣂ੐ᬺ㧔ࡃࠗࠝࡑࠬ㧕㧛਻Ꮊ⊒ࡆ࡟࠶ࠫ࠹ࠢࡁࡠࠫ࡯᭴▽ߦะߌߚ┻߆ ࠄߩࡃࠗࠝࠛ࠲ࡁ࡯࡞ᄌ឵ߩᛛⴚ㐿⊒

࡮ฃ⸤⎇ⓥ㧔䋨ᩣ㧕ࠅࠀ߁ߖ߈㧕ޔᐕޔ౞ޔⅣႺ⋭ ᐔᚑᐕᐲ

࿾⃿᷷ᥦൻኻ╷ᛛⴚ㐿⊒੐ᬺ㧔ᴒ✽↥♧Ᵽ߆ࠄߩΆᢱ↪ࠛ࠲ࡁ࡯࡞↢↥ࡊࡠ

࠮ࠬ㐿⊒෸߮'╬ታ⸽ታ㛎㧛ᴒ✽↥♧Ᵽߩㆡ↪ᕈ╬ߦ㑐ߔࠆ⎇ⓥ㐿⊒㧕

࡮⑼ቇᛛⴚᝄ⥝⺞ᢛ⾌㧔ᾢᧄᄢቇઍ⴫㧦ṚᎹవ↢㧕ޔᐕޔ౞ޔᐩ

⾰ᯏ⢻ߩᓸ↢‛ቇ⊛⹏ଔߣᡷༀᛛⴚߩᬌ⸛

࡮⑼⎇⾌ޔᐕޔ౞ޔᄤὼࡎ࡞ࡕࡦ㒰෰ࠍ฽߻ኅ⇓♮ዩ࡮↢ߏߺᷙ

ว‛ߩ㜞ല₸ࠨ࡯ࡑ࡞࡝ࠨࠗࠢ࡞ᛛⴚߩ㐿⊒

࡮౒ห⎇ⓥ㧔࿖┙ᄢቇᴺੱ⑔ፉᄢቇ㧕ޔᐕޔ౞ޔ᳓↢ᬀ‛ᱷᷲࡃ

ࠗࠝࡑࠬ߆ࠄߩࠕ࡞ࠦ࡯࡞⊒㉂ߦ㑐ߔࠆᛛⴚ㐿⊒⎇ⓥ

࡮౒ห⎇ⓥ㧔᧲੩ⅽᣁ㧔ᩣ䋩㧕ޔᐕޔ౞ޔ↢ߏߺࠍਥේᢱߣߒߚ ࠕ࡞ࠦ࡯࡞↢↥ࡊࡠ࠮ࠬ㐿⊒߅ࠃ߮ޔᒰ⹥ࠪࠬ࠹ࡓߦ᦭ലߥࠕ࡞ࠦ࡯࡞⊒㉂

㉂Უߩ⢒⒳ߦ㑐ߔࠆ౒ห⎇ⓥ

࡮౒ห⎇ⓥ㧔䋨ᩣ㧕ࡒ࠷ࠞࡦࠣ࡞࡯ࡊᧄ␠㧕ޔᐕޔ౞ޔਛ࿖࿕૕

㈶㉄⊒㉂ߦ߅ߌࠆ․ᓽ⊛㚅᳇↢ᚑߩᓸ↢‛ቇ⊛ࡔࠞ࠾࠭ࡓ⸃ᨆ

ฎᎹ ᙗᴦ

㧨ⅣႺ⋭ᑄ᫈‛ಣℂ╬⑼ቇ⎇ⓥ⾌⵬ഥ㊄㧪

ޟࡃࠗࠝࠟࠬൻࡊ࡜ࡦ࠻ឃ᳓ਛߩ㜞Ớᐲࠕࡦࡕ࠾ࠕߩ /#2#0#//1: ࡂࠗࡉ࡝࠶

࠼ಣℂᛛⴚߩ㐿⊒ޠޔᐔᚑ ᐕᐲ ਁ౞ޔᐔᚑ ᐕ㨪 ᐕ 㧨౒ห⎇ⓥ㧪

࡮㑐᧲ࠟࠬ㐿⊒ࢃ ਁ౞

࡮⨶ේⅣႺࠛࡦࠫ࠾ࠕ࡝ࡦࠣࢃ ਁ౞

࡮ࢃ࠲ࠢࡑ ਁ౞

㧨ᅑቇነ㒝㊄㧪

࡮ࢃ ࠢ࡜࡟ޔ ਁ౞

࡮ࠝ࡞ࠟࡁࢃޔ ਁ౞

࡮ࢃ⍹၂ޔ ਁ౞

࡮,GQP6GEJ%Q.VF㧔㖧࿖㧕 ਁ౞

࡮ᣣ┙ࡊ࡜ࡦ࠻ࢃޔ ਁ౞

Ꮉ⿧ ଻ᓼ

࡮㧔⽷㧕೨↰⸥ᔨᎿቇᝄ⥝⽷࿅⎇ⓥഥᚑ ᐕ ਁ౞ᄥ㓁శ㓸శࠪࠬ࠹

ࡓߣ⊒శ࠳ࠗࠝ࡯࠼ࠍశḮߣߔࠆើ⊒ᕈ᦭ᯏ㉄߆ࠄߩశวᚑ᳓⚛↢ᚑߦ㑐ߔ ࠆၮ␆⊛⎇ⓥ

࡮᡽╷ഃㅧ⎇ⓥᢎ⢒࠮ࡦ࠲࡯ࡊࡠࠫࠚࠢ࠻⎇ⓥ⺖㗴 ᐕޯ ᐕ ਁ

౞ᾢᧄᵹၞߢߩ᳓ᓴⅣ଻ోߣߘߩஜోߥ᳓೑↪ߦ㑐ߔࠆ⎇ⓥ

࡮ᾢᧄᏒ᳓㆏ዪ ᐕޯ ᐕ ਁ౞ᾢᧄᏒ

᎑↰ ⚐

࡮⑼⎇⾌ၮ⋚⎇ⓥ䋨㧮䋩䋨ᶏᄖቇⴚ⺞ᩏ䋩 ᐔᚑ㧙ᐕᐲ ᧲ࠕࠫࠕᴪጯၞߦ߅

ߌࠆ࿾ਅ᳓ᵹ಴ߦ઻߁὇⚛࡮ᩕ㙃Ⴎ㘃⽶⩄㊂ߩ⹏ଔ㧚⼱ญ⌀ੱ㧔ઍ⴫㧕ޔ⫱

↢ବᢘޔᓼ᳗᦮␽ޔችጟ㇌છޔᒛ ഹޔ᎑↰ ⚐ޔዊ㊁ኹ⌀৻ ජ౞

࡮⑼⎇⾌ ၮ⋚⎇ⓥ㧔%㧕ᐔᚑ㧙ᐕᐲ ࡑ࡞࠴࡮࠻࡟࡯ࠨ࡯ᚻᴺߦࠃࠆᵹၞ

᳓࡮‛⾰ᓴⅣࡊࡠ࠮ࠬߩࡕ࠺࡞ൻߦ㑐ߔࠆ⎇ⓥ㧚 ㄞ᧛⌀⾆㧔ઍ⴫㧕ޔ᎑↰

⚐ ජ౞

࡮⑼⎇⾌ ၮ⋚⎇ⓥ㧔

㧕ᐔᚑ㧙ᐕᐲ ⇣Ᏹ㑆㓗᳓࿶ጀߩ⊒↢࡮਄᣹ࡔࠞ

࠾࠭ࡓߣᐢၞ࿾ਅ᳓ᵹേޔ⥄ὼἴኂߦ෸߷ߔᓇ㗀 ↰ਛ๺ᐢ㧔ઍ⴫㧕ޔች↰

㓶৻㇢ޔජᧁ⦟㓷ᒄޔᓼ᳗᦮␽ޔ੹ጟᾖ༑ޔਛ↰⧷ੑޔ᎑↰ ⚐ޔ㊄᛬⵨มޔ

⍹↰ Პޔ㚍ේ଻ౖޔජ౞

࡮ㇺၔᏒฃ⸤⎇ⓥ⾌ ᐕᐲޔઍ⴫ ජ౞㧘 ޡㇺၔ⋆࿾ߩ࿾ਅ᳓ᵹേ࡮ᓴⅣ ᯏ᭴⸃ᨆޢ

࡮✚ว࿾⃿ⅣႺቇ⎇ⓥᚲ ᐕᐲ౒ห⎇ⓥޔಽᜂ ජ౞㧘ޡㇺᏒߩ࿾ਅⅣ Ⴚߦᱷࠆੱ㑆ᵴേߩᓇ㗀ޢ

࡮⾗Ḯ࡮⚛᧚ቇળޔᷓㇱ࿾⾰ⅣႺ⺞ᩏ⸃ᨆᛛⴚ૕♽ൻᆔຬળ ฃ⸤⎇ⓥ ޡ࿾ਅ

᳓ߩ࿾⃿ൻቇ⊛⺞ᩏߦ㑐ߔࠆ⎇ⓥޢޔಽᜂ ජ౞

࡮ᩭΆᢱࠨࠗࠢ࡞ᯏ᭴వ┵ၮ␆⎇ⓥഥᚑᐔᚑᐕᐲ Ⴎ⚛቟ቯห૏૕Ყࠍ↪

޿ߚ᳓ℂ࿾⾰᭴ㅧࡕ࠺࡞⹏ଔᛛⴚߩ㐿⊒㧚ᓼ᳗᦮␽䋨ઍ⴫䋩ޔ᎑↰ ⚐ޔ੗ዥ

⵨ੑޔዊ㊁ ⺈ޔජ౞

࡮ᆔછ⚻ℂ㊄ 㧝ઙ 㧔 ජ౞㧕 ዊᳰ స᣿

࡮⑼ቇ⎇ⓥ⾌ ၮ⋚⎇ⓥ

㧔ઍ⴫㧕㧦

ජ౞ 䌛⛮⛯㨉

࡮ᣣᧄේሶജ⎇ⓥ㐿⊒ᯏ᭴ వⴕၮ␆Ꮏቇ⎇ⓥ㧔ઍ⴫㧕㧦

ජ౞

࡮䋨⽷䋩ᣣᧄ㋶ᬺᝄ⥝ળ⹜㛎⎇ⓥ㧔ઍ⴫㧕㧦

ජ౞

࡮ነ㒝㊄㧦᳃㑆ડᬺ࡮䋨␠䋩⾗Ḯ࡮⚛᧚ቇળࠃࠅ㧘⸘

ජ౞

࡮౒ห⎇ⓥ⾌㧦࿖┙ᭂ࿾⎇࡮੩ㇺᄢቇ㒐ἴ⎇㧔ಽᜂ㧕㧘⸘

ජ౞

ṚᎹ ᷡ

Ԙᣣᧄቇⴚᝄ⥝⑼ቇ⎇ⓥ⾌⵬ഥ㊄

㧕ṚᎹᷡ㧔ᾢᄢઍ⴫㧕㧚ᢥㇱ⑼ቇ⋭⑼ቇᛛⴚᝄ⥝⺞ᢛ⾌㧦㊀ⷐ⺖㗴⸃᳿ဳ

⎇ⓥ㧘 ޟ᦭᣿ᶏ↢‛↢ᕷⅣႺߩਇୄ⍑ဳౣ↢ߣታ⸽⹜㛎䋨 ᐕᐲ ජ౞䋩

ԙ ᅑቇነઃ㊄

ઙ ౞㧔ᴪጯၞⅣႺ⑼ቇᢎ⢒⎇ⓥ࠮ࡦ࠲࡯࡮ᢎ᝼㧧ṚᎹ ᷡ㧕 Ԛ౒ห⎇ⓥ

㧕ṚᎹᷡ㧚 㧔ࠕࠫࠕࡊ࡜ࡦ࠾ࡦࠣࢃ㧕ᐔᚑޯ⛮⛯ਛ䋨ජ౞䋩ᐕޟ᦭

᣿ᶏၞߦ߅ߌࠆ⥄ὼⅣႺߩ⹏ଔߣ଻ోߦ㑐ߔࠆ⎇ⓥޠ

ၔ ᤘౖ

࡮ਃ੗㊄ዻ㋶ᬺᩣᑼળ␠ߣߩ౒ห⎇ⓥᐕਁ౞Ớෘ㊄ዻႮṁᶧਛߩ ᓸ㊂ࡈ࠶ൻ‛ࠗࠝࡦߩ㒰෰ࠍ⋡⊛ߣߔࠆ㜞ᯏ⢻ㆬᛯๆ⌕೷ߩ㐿⊒ߦ㑐ߔࠆ⎇

ⓥ

ᴡේ ᱜᵏ

࡮ᐔᚑ ᐕᐲ⑼ቇ⎇ⓥ⾌⵬ഥ㊄㧔ၮ⋚⎇ⓥ㧔%䋩㧕ઍ⴫

ޟ฽Ꮧ࿯ࡑࠣࡀࠪ࠙ࡓว㊄ࠬࠢ࡜࠶ࡊ߆ࠄߩᏗ࿯㘃ߩ࿁෼ޠ㧔 ජ౞㧕

ᐔᚑ19ᐕᐲ⎇ⓥᚑᨐႎ๔

⎇ⓥ⺖㗴ฬ㧦ⅣႺᳪᨴ‛⾰ߩ↢‛ࡕ࠾࠲࡝ࡦࠣ

ᚲዻ࡮᳁ฬ 㧦⥄ὼ⑼ቇ⎇ⓥ⑼࡮ⶄวᣂ㗔ၞ⑼ቇኾ᡹࡮ർ㊁ஜ࡮቟ㇱ⋿৻

1. ⎇ⓥ⋡⊛࡮⋡ᮡ

H15ᐕᐲޯ19ᐕᐲߦ߅ߌࠆ⋡⊛࡮⋡ᮡ

ᴡᎹ߿ᶏߦሽ࿷ߔࠆⅣႺᳪᨴ‛⾰ߩਛߢޔ․ߦౝಽᴲ♽ߦᓇ㗀ࠍਈ߃ࠆൻቇ‛⾰ߩߎ ߣࠍౝಽᴲᠣੂ‛⾰ߣ๭߱ޕߎߩౝಽᴲᠣੂ‛⾰߇෸߷ߔ↢‛߳ߩ૞↪ᯏ᭴ߦߟ޿ߡߪ ᧂߛߦ᣿ࠄ߆ߦߐࠇߡ޿ߥ޿ߚ߼ޔ᦭ലߥࡕ࠾࠲࡝ࡦࠣࠪࠬ࠹ࡓߩ㐿⊒ߦߪ⥋ߞߡ޿ߥ

޿ߩ߇⃻⁁ߢ޽ࠆޕߘߎߢᧄ⎇ⓥߢߪޔౝಽᴲᠣੂ‛⾰߇෸߷ߔ↢‛߳ߩᓇ㗀ࡔࠞ࠾࠭

ࡓࠍ᣿ࠄ߆ߦߔࠆߣหᤨߦޔᴡᎹ߿ᶏߦሽ࿷ߔࠆౝಽᴲᠣੂ‛⾰ࠍ⹏ଔߢ߈ࠆ↢‛ࡕ࠾

࠲࡝ࡦࠣࠪࠬ࠹ࡓߩ㐿⊒ࠍⴕ߁ޕߐࠄߦޔⅣႺᳪᨴ‛⾰એᄖߩⅣႺⷐ࿃䋨᳓᷷ߥߤ䋩߇෸

߷ߔ↢‛߳ߩᓇ㗀෸߮૞↪ᯏ᭴ߦߟ޿ߡ߽⸃ᨆߒޔ↢‛߇↢⢒ߔࠆⅣႺࠍ✚ว⊛ߦ⹏ଔ ߢ߈ࠆ↢‛ࡕ࠾࠲࡝ࡦࠣࠪࠬ࠹ࡓߩ㐿⊒ࠍ߼ߑߔޕ

H19ᐕᐲߩ⋡⊛࡮⋡ᮡ

ᧄᐕᐲߪޔⅣႺⷐ࿃ߩਛߢޔ․ߦ᳓᷷߇෸߷ߔ↢‛߳ߩᓇ㗀ࡔࠞ࠾࠭ࡓߦߟ޿ߡ⸃ᨆ ߔࠆޕᚒޘߪޔ᦭᣿ᶏߩᴪጯၞ䋨᳓᷷ߪ⚂10-28͠䋩ߦ↢ᕷߔࠆᶏ↥㝼ߢ޽ࠆࡅ࡜ࡔ 䋨Paralichthys olivaceus䋩ߩㆮવ⊛㓽䋨XX䋩߇ޔᕈಽൻᤨᦼߦ18͠ߢ㘺⢒ߔࠆߣ100㧑㓽ߦߥ ࠆ߇ޔ27͠ߢ㘺⢒ߔࠆߎߣߢ100㧑㓶߳ߣᕈォ឵ߔࠆߎߣࠍ᣿ࠄ߆ߦߒߡ޿ࠆ䋨Kitano et al., 1999䋩ޕߐࠄߦޔ㜞᳓᷷㘺⢒ߦࠃࠆᕈォ឵ߦ઻޿ޔࠕࡠࡑ࠲࡯࠯䋨ࠛࠬ࠻ࡠࠥࡦวᚑ㉂

⚛䋩ㆮવሶߩ⊒⃻߇ᛥ೙ߐࠇࠆ৻ᣇޔMIS䋨Mullerian inhibiting substance䋩ㆮવሶߩ⊒⃻߇⺃

ዉߐࠇࠆߎߣ߇ಽ߆ߞߡ޿ࠆ䋨Kitano et al., 2000; Yoshinaga et al., 2004䋩ޕߘߎߢ੹ᐕᐲߪޔ 㜞᳓᷷ࠬ࠻࡟ࠬߦࠃࠅวᚑ㊂߇਄᣹ߔࠆಽᴲᕈ࿃ሶߦ⌕⋡ߒߡޔߘߩᓇ㗀ࠍ⸃ᨆߒߚޕ

ᚒޘߪޔ⑺ޯ౻᷷ᐲ߇ૐਅߔࠆߣࠗࡕ࡝ⴊਛߩࡊࡠ࡜ࠢ࠴ࡦࡎ࡞ࡕࡦ߇਄᣹ߒޔ♖Ꮍ ߦࠕࡐ࠻࡯ࠪࠬࠍᒁ߈⿠ߎߔߎߣࠍ⊒⷗ߒߚ㧔Yazawa et al., 2000㧕ޕߘߎߢޔࠗࡕ࡝♖

Ꮍߦ߅ߌࠆࠕࡐ࠻࡯ࠪࠬᯏ᭴ߦߟ޿ߡޔ࡝ࠟࡦ࠼߇ߤߩࠃ߁ߦߒߡฃኈ૕ࠍᵴᕈൻߔࠆ ߆ޔ⚦⢩ౝߢߤߩࠃ߁ߦࠪࠣ࠽࡞߇વࠊࠆ߆ޔᱫߩታⴕㆊ⒟ߪߤߩࠃ߁ߥ߽ߩ߆ޔߦߟ

޿ߡ⺞ߴࠆߎߣࠍ⋡⊛ߣߔࠆޕ੹ᐕᐲߪޔ♖ේ⚦⢩ౝߩ᛫ࠕࡐ࠻࡯ࠪࠬ࿃ሶߢ޽ࠆRNA

⚿ว࠲ࡦࡄࠢ⾰䋨nRBP䋩ߩ⊒⃻ߣ૞↪ߦߟ޿ߡ⺞ߴߚޕ߹ߚޔⅣႺᲥᕈ‛⾰ߩ♖ሶᒻᚑߦ ኻߔࠆᓇ㗀ߦߟ޿ߡਔ↢㘃♖Ꮍၭ㙃♽ࠍ↪޿ߡ⺞ߴߚޕ

2. ᐔᚑ19ᐕᐲߩ⎇ⓥᚑᨐ

(1)㜞᳓᷷㘺⢒ߦࠃࠅޔࡅ࡜ࡔㆮવ⊛㓽䋨XX䋩߇㓶߳ߣᕈォ឵ߔࠆಽሶᯏ᭴

ࡅ࡜ࡔߩXX୘૕ߪޔᕈಽൻᤨᦼߩ㜞᳓᷷䋨27͠䋩㘺⢒ߦࠃࠅޔ↢ᱺ⣼ߢߩ㓽․⇣⊛ㆮ વሶ䋨ࠕࡠࡑ࠲࡯࠯ޔFoxl2䋩ߩ⊒⃻߇ᛥ೙ߐࠇޔ㓶․⇣⊛ㆮવሶ䋨MIS䋩ߩ⊒⃻߇⺃ዉߐࠇ ߡ㓶߳ߣᕈಽൻߔࠆߎߣ߇᣿ࠄ߆ߣߥߞߡ޿ࠆޕᤓᐕᐲޔᕈಽൻᤨᦼߩ↢ᱺ⣼ߩེቭၭ

㙃♽ߦ߅޿ߡޔ18͠ߢၭ㙃ߒߡ߽ޔ27͠ߢၭ㙃ߒߡ߽ޔࠕࡠࡑ࠲࡯࠯෸߮Foxl2 mRNA ߩ⊒⃻߇ቢోߦߪᛥ೙ߐࠇߕޔMIS mRNAߩ⊒⃻߇ోߊ⺃ዉߐࠇߥ޿ߎߣ߇ಽ߆ߞߚޕ ߎߩߎߣߪޔ㜞᳓᷷ߪ↢ᱺ⣼ߦߪ⋥ធ૞↪ߖߕߦޔ↢ᱺ⣼એᄖ߆ࠄಽᴲߔࠆ࿃ሶࠍ੺ߒ ߡ㓶߳ߩᕈಽൻࠍ⺃ዉߒߡ޿ࠆߩߢߪߥ޿߆ߣ⠨߃ࠄࠇߚޕߘߎߢ੹ᐕᐲߪޔ㜞᳓᷷ࠬ

࠻࡟ࠬߦࠃࠅวᚑ㊂߇਄᣹ߔࠆ࿃ሶߩ৻ߟߢ޽ࠆࠦ࡞࠴࠱࡞ߦ⌕⋡ߒޔࡅ࡜ࡔᕈಽൻߦ ߅ߌࠆߎߩᓇ㗀ࠍ⸃ᨆߒߚޕEIAᴺߦࠃࠅࠦ࡞࠴࠱࡞㊂ࠍ᷹ቯߒߚ⚿ᨐޔᕈಽൻᤨᦼߦ ߅޿ߡޔ㜞᳓᷷ಣℂߒߚXX୘૕ߪㅢᏱಣℂ୘૕ߦᲧߴߡޔࠦ࡞࠴࠱࡞㊂߇᦭ᗧߦ㜞޿

ߎߣ߇ಽ߆ߞߚޕᰴߦޔࠦ࡞࠴࠱࡞ࠍᛩਈߒߚXX୘૕ߩᕈᲧࠍ⺞ߴߚ⚿ᨐޔ㜞Ớᐲಣ ℂ⟲ߦ߅޿ߡ 50㧑ߩ㓶ൻ₸ࠍ␜ߒߚޕߐࠄߦޔ↢ᱺ⣼ߩེቭၭ㙃♽ߦࠦ࡞࠴࠱࡞ࠍᷝ

ടߒߚ⚿ᨐޔࠕࡠࡑ࠲࡯࠯෸߮Foxl2 mRNAߩ⊒⃻߇ᛥ೙ߐࠇޔMIS mRNAߩ⊒⃻߇⺃

ዉߐࠇߚޕߎࠇࠄߩߎߣ߆ࠄޔࠦ࡞࠴࠱࡞ߪ㜞᳓᷷ߦࠃࠆࡅ࡜ࡔߩ㓶ൻߦᷓߊ㑐ਈߒߡ

޿ࠆน⢻ᕈ߇␜ໂߐࠇߚޕ

(2)ࠗࡕ࡝♖Ꮍߢߩࡊࡠ࡜ࠢ࠴ࡦߦࠃࠆࠕࡐ࠻࡯ࠪࠬߦ߅ߌࠆRNA⚿ว࠲ࡦࡄࠢ⾰ߩᓎ

ഀ

ࠗࡕ࡝♖Ꮍߢߪޔᷫᢙಽⵚ㐿ᆎ߆ࠕࡐ࠻࡯ࠪࠬ߆ߩㆇ๮ߩጘ〝߇╙㧣਎ઍ♖ේ⚦⢩ߦ

޽ࠅޔߘߩㆇ๮᳿ቯߪޟ↢ޠߩࠪࠣ࠽࡞ߢ޽ࠆỹ⢩ೝỗࡎ࡞ࡕࡦ䋨FSH䋩ߣޟᱫޠߩࠪࠣ

࠽࡞ߢ޽ࠆࡊࡠ࡜ࠢ࠴ࡦ䋨PRL䋩ߣߩࡃ࡜ࡦࠬߦࠃߞߡ⺞▵ߐࠇߡ޿ࠆ䋨Abe, 2004䋩ޕߔߥ ࠊߜޔ╙㧣਎ઍ♖ේ⚦⢩ߪ PRL ߩⴊਛỚᐲ߇㜞ߊߥࠆ⑺ޯ౻ߢߪࠕࡐ࠻࡯ࠪࠬࠍ⺃ዉ ߔࠆߩߦኻߒߡޔPRLߩⴊਛỚᐲ߇ૐߊߥࠆᤐߢߪჇᱺޔಽൻࠍଦㅴߔࠆޕᚒޘߪޔPRL ߦᔕ╵ߒߡmRNAߩ⊒⃻߇ᷫዋߔࠆࠢࡠ࡯ࡦߩ৻ߟߣߒߡRNA⚿ว࠲ࡦࡄࠢ⾰䋨nRBP䋩 ࠍࠗࡕ࡝♖Ꮍ↱᧪ߩ5321୘ߩcDNA߆ࠄࡑࠗࠢࡠࠕ࡟ࠗᴺߢන㔌ߒߚޕ৻⥸ߦޔRNA

⚿ว࠲ࡦࡄࠢ⾰ߪ mRNA ߩ⠡⸶ޔࡊࡠ࠮ࠪࡦࠣߥߤࠍ೙ᓮߔࠆߎߣ߇⍮ࠄࠇߡ޿ࠆޕ nRBP࠲ࡦࡄࠢ⾰ߩ⊒⃻ࠍ⺞ߴߚߣߎࠈޔ╙㧣਎ઍ♖ේ⚦⢩ߩ⚦⢩⾰ߢ⊒⃻ߒߡ޿ࠆߎ ߣޔPRLߩ૞↪ߦࠃߞߡࠕࡐ࠻࡯ࠪࠬࠍ⿠ߎߒߡ޿ࠆ⚦⢩ߛߌߢߥߊ⿠ߎߒߡ޿ߥ޿⚦

⢩ߢ߽ᷫዋߒߡ޿ߚޕ߹ߚޔ♖Ꮍߩ↢ᱺ⚦⢩ᩭߣ PRL ࠍᵈ኿ߒߚࠗࡕ࡝ߩ♖Ꮍ߆ࠄ⺞

⵾ߒߚ⚦⢩⾰᛽಴ᶧࠍ෻ᔕߐߖߚߣߎࠈޔPRLߦࠃࠆࠕࡐ࠻࡯ࠪࠬ߇ή⚦⢩♽ߢ߽⺃ዉ ߐࠇࠆߎߣ߇ಽ߆ߞߚޕߎߩή⚦⢩♽ߦnRBP࠲ࡦࡄࠢ⾰ࠍᷝടߔࠆߣޔࠕࡐ࠻࡯ࠪࠬ

߇ᛥ೙ߐࠇߚޕᰴߦޔnRBP߇޿߆ߦࠕࡐ࠻࡯ࠪࠬࠍᛥ೙ߔࠆ߆ࠍ᣿ࠄ߆ߦߔࠆߚ߼ߦޔ nRBPߦ⚿วߔࠆprogrammed cell death protein 4䋨Pdcd4䋩ߩRNAࠍ౒఺∉ᴉ㒠ߦࠃࠅࠗࡕ

࡝♖Ꮍ߆ࠄන㔌ߒߚޕPdcd4ߪࠕࡐ࠻࡯ࠪࠬߩଦㅴߦ㑐ਈߔࠆ߽ߩߢޔߘߩmRNAߩ⊒

⃻ߪPRLࠍࠗࡕ࡝ߦᵈ኿ߒߡnRBPߩ⊒⃻߇߶߷ᶖᄬߔࠆ24ᤨ㑆ᓟߦ৻ㆊ⊛ߦ਄᣹ߒ ߡ޿ߚޕએ਄ߩ⚿ᨐߪޔnRBP߇Pdcd4ߩmRNAߦ⚿วߔࠆߎߣߢ᛫ࠕࡐ࠻࡯ࠪࠬᵴᕈ ࠍ⊒ើߒߡ޿ࠆ߇ޔPRLߦࠃࠆnRBPߩ⊒⃻ૐਅ߇╙㧣਎ઍ♖ේ⚦⢩ߦࠕࡐ࠻࡯ࠪࠬࠍ

⺃ዉߔࠆߣ޿߁ㆇ๮ࠍ᳿ቯઃߌߡ޿ࠆߎߣࠍ␜ໂߒߚޕ

(3)ࠗࡕ࡝↢ᱺ⚦⢩ߩಽൻߦኻߔࠆࡆࠬࡈࠚࡁ࡯࡞Aߩᓇ㗀

ࡐ࡝ࠞ࡯ࡏࡀ࡯࠻᮸⢽߿ࠛࡐࠠࠪ᮸⢽ߩේᢱߣߒߡ࿖ౝߢ߽ᐕ㑆50 ਁ࠻ࡦ߇↢↥ߐ ࠇߡ޿ࠆࡆࠬࡈࠛࡁ࡯࡞A 㧔એਅޔBPA ߣ⇛ߔ㧕ߪޔCD ߿⛎㘩↪㘩ེ╬ߩᚒޘ ߦり ㄭߥ⵾ຠߦ↪޿ࠄࠇߡ޿ࠆ߇ޔࠬ࠹ࡠࠗ࠼ࡎ࡞ࡕࡦߩ৻⒳ߢ޽ࠅޔߘߩฃኈ૕㧔ࠛࠬ࠻

ࡠࠥࡦฃኈ૕㧕ࠍ੺ߒߡ․ቯߩDNA ߩวᚑࠍᵴᕈൻߔࠆࠛࠬ࠻ࡠࠫࠚࡦ᭽૞↪ࠍ␜ߒ ߡ↲⁁⣼⇣Ᏹߥߤࠍᒁ߈⿠ߎߔ੐߇⍮ࠄࠇߡ޿ࠆޕ

ౝಽᴲᠣੂ‛⾰ߩ↢ᱺ⚦⢩ಽൻߦ෸߷ߔᓇ㗀ࠍ⺞ߴࠆ⋡⊛ߢޔ᳓↢േ‛ߢ♖ሶಽൻᯏ

᭴߇⹦ߒߊ⺞ߴࠄࠇߡ޿ࠆࠗࡕ࡝ߩ♖Ꮍࠍ↪޿ߡޔBPAߩᓇ㗀ࠍ⺞ߴߚޕᚑᾫ㓶ࠃࠅ♖

Ꮍࠍන㔌ߒBPAࠍ1ppm, 10ppm, 100ppmߩỚᐲߢᷝടߒߡၭ㙃ߒޔ♖ේ⚦⢩߳ߩᓇ㗀 ࠍ⺞ߴߚޕߘߩ⚿ᨐޔ1ppmߢߪ߶ߣࠎߤᓇ㗀ߪ⷗ࠄࠇߥ߆ߞߚ߇ޔ10ppmએ਄ߩỚᐲ ߢߪޔỚᐲଐሽ⊛ߦᱫṌߔࠆ♖ේ⚦⢩߇Ⴧടߒߚޕ100ppm ߢߪᚑᾫߒߚ♖⚦⢩ߪᓇ㗀 ࠍฃߌߥ߆ߞߚ߇ޔ࠮࡞࠻࡝⚦⢩ࠍᱷߒߡ߶ߣࠎߤߩ♖ේ⚦⢩ߣ♖Უ⚦⢩ߩᩭ߇ᶖᄬߒ ߚޕߎࠇࠄߩ⚿ᨐࠃࠅޔBPAߪ♖ේ⚦⢩ߣ♖Უ⚦⢩ߦኻߒߡㆬᛯ⊛ߦ⚦⢩Ქᕈ߇޽ࠆߎ ߣޔ࠮࡞࠻࡝⚦⢩ߪ♖ේ⚦⢩ߣ♖Უ⚦⢩ߦᲧߴߡᓇ㗀ࠍฃߌ߇ߚ޿ߎߣ߇᣿ࠄ߆ߦߥߞ ߚޕ㧔ߎߩታ㛎ߪ౎ઍ㜞ኾߩ᪢ᧄዏ↵ำߣ㊄↰ᾖᄦᢎ᝼߇ⴕ޿ޔ⑳߇ഥ⸒ࠍਈ߃ߚ㧕

3. ᚑᨐߩ㆐ᚑᐲ

੹ᐕᐲߪޔ㜞᳓᷷ߦࠃࠆࡅ࡜ࡔߩ㓶ൻࠍ⺃ዉߒߡ޿ࠆน⢻ᕈ߇޽ࠆಽᴲᕈ࿃ሶ䋨ࠦ࡞

࠴࠱࡞䋩ߩหቯߦᚑഞߒߚޕ߹ߚޔࠗࡕ࡝♖Ꮍߢ♖ේ⚦⢩ౝߢ⊒⃻ߔࠆRNA⚿ว࠲ࡦࡄ

ࠢ⾰߇ޔ╙㧣਎ઍ♖ේ⚦⢩ߢߩߺ⊒⃻ߒޔࡊࡠ࡜ࠢ࠴ࡦߦࠃߞߡᶖᄬߔࠆߎߣޔ᛫ࠕࡐ

࠻࡯ࠪࠬᵴᕈࠍ᦭ߒߡ޿ࠆߎߣޔࠕࡐ࠻࡯ࠪࠬߩଦㅴߦ㑐ਈߔࠆPdcd4ߣ⚿วߔࠆߎߣޔ ࠍ᣿ࠄ߆ߦߒߚߩߢޔࡊࡠ࡜ࠢ࠴ࡦߦࠃࠆࠕࡐ࠻࡯ࠪࠬ⺃⿠ᯏ᭴ߦߟ޿ߡℂ⸃߇ᷓ߹ߞ ߚޕ߹ߚޔࠗࡕ࡝↢ᱺ⚦⢩ߩಽൻߦኻߔࠆࡆࠬࡈࠚࡁ࡯࡞Aߩᓇ㗀ߦߟ޿ߡၭ㙃♽ࠍ↪

޿ߡ⺞ߴߚޕߎࠇࠄߩ⚿ᨐ߆ࠄޔ⋡ᮡߪචಽ㆐ᚑߢ߈ߚߣ⠨߃ࠄࠇࠆޕ

4㧚ᐔᚑ19ᐕᐲߩ⎇ⓥ⾌ขᓧ⁁ᴫ

(1)ၮ⋚⎇ⓥ䋨B䋩, 2006-2008ᐕ, 550ਁ౞䋨ᐔᚑ19ᐕᐲಽ䋩, ♖ሶಽൻߦ߅ߌࠆᣂⷙࡄ࡜ࠢ

࡝ࡦ࿃ሶ࠾ࡘ࡯࡟ࠡࡘ࡝ࡦߩᯏ⢻

(2) ℂ⎇ߣߩ౒ห⎇ⓥ⾌, 2007ᐕᐲ 350ਁ౞ޔ↢ᱺ∔ᖚࡕ࠺࡞േ‛ߩ㐿⊒

(3)․ቯ㗔ၞ⎇ⓥ䋨౏൐⎇ⓥ䋩, 2007-2009ᐕ, 330ਁ౞䋨ᐔᚑ19ᐕᐲಽ䋩, ᷷ᐲଐሽᕈᕈ

᳿ቯߩಽሶࡔࠞ࠾࠭ࡓ

(4)⧯ᚻ⎇ⓥB, 2007-2009ᐕ, 200ਁ౞䋨ᐔᚑ19ᐕᐲಽ䋩, ࡔ࠳ࠞᕈಽൻߦ߅ߌࠆ⚦⢩Ⴧᱺ

࿃ሶߩᓎഀ

5㧚ᐔᚑ19ᐕᐲߩ⎇ⓥ⊒⴫ޔ․⸵⁁ᴫ (1) ේ⪺⺰ᢥߦࠃࠆ⊒⴫

࿖㓙⹹㧔᰷ᢥ㧕

Ԙ Shiraishi E., Yoshinaga N., Miura T., Yokoi H., Wakamatsu Y., Abe S. and Kitano T.

Mullerian inhibiting substance is required for germ cell proliferation during early gonadal differentiation in medaka (Oryzias latipies). Endocrinology149, 1813-1819, 2008.

ԙYazawa T., Uesaka M., Inaoka Y., Mizutani T., Sekiguchi T., Kajitani T., Kitano T., Umezawa A. and Miyamoto K. Cyp11b1 is induced in the murine gonad by luteinizing hormone/ human chorionic gonadotropin and involved in the production of

11-ketotestosterone, a major fish androgen; conservation and evolution of androgen metabolic pathway. Endocrinology(In press).

ԚShimada H., Yasutake A., Hirashima T., Takamure Y., Kitano T., Waalkes M.P. and Imamura Y. Strain difference of cadmium accumulation by liver slices of inbred Wistar-Imamichi and Fischer 344 rats. Toxicology in Vitro22, 338-343, 2008.

ԛYamaguchi T., Yamaguchi S., Hirai T. and Kitano T. Follicle-stimulating hormone signaling and Foxl2 are involved in transcriptional regulation of aromatase gene during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus.

Biochemical and Biophysical Research Communications 359, 935-940, 2007.

Ԝ Oba Y., Shimasaki Y., Oshima Y., Satone H., Kitano T., Nakao M., Kawabata S. and Honjo T. Purification and characterization of tributyltin binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus.J. Biochemistry 142, 229-238, 2007.

ԝ Hano T., Oshima Y., Kim S. G., Satone H., Oba Y., Kitano T., Inoue S., Shimasaki Y. and Honjo T. Tributlyltin causes abnormal development in embryo of medaka, Oryzias latipes.

Chemosphere 69, 927-933, 2007.

Ԟ Kawasaki T., Imura, F., Nakada, A., Kubota, H., Sakamaki, K., Abe, S.-I. and Takamune, K.

(2006) Functional demonstration of the ability of a primary spermatogonium as a stem cell by tracing a single cell destiny in Xenopus laevis. Development Growth and

Differentiation, 48, 525-535.

ԟ Jin, Y., Uchida, I., Eto, K., Kitano, T. and Abe, S.-I. (2008) Size-selective junctional barrier and Ca2+-independent cell adhesion in the testis of Cynops pyrrhogaster: expression and function of occludin. Molecular Reproduction and Development, 75, 202-216.

Ԡ Abe K., Eto K. and Abe S.-I. (2008) Epidermal growth factor mediates spermatogonial proliferation in newt testis. Reprod. Biol. Endocr, (in press).

(2) ේ⪺⺰ᢥએᄖߦࠃࠆ⊒⴫

⪺ᦠ

Ԙ Kitano T. and Abe S. Involvement of endocrine and environmental factors in gonadal sex differentiation in gonochoristic fish. In Fish Reproduction, pp 421-434, 2007.

ԙ቟ㇱ⋿৻ ╙㧢┨ ⣄ᬁേ‛ߩ♖ሶಽൻ̅ࠗࡕ࡝♖Ꮍࠍࡕ࠺࡞ߦߒߡ࡯ޔ ਎♿ߩ

േ‛⑼ቇޕ╙ Ꮞޟᕈߣ↢ᱺޠ㧔቟ㇱ⋿৻ޔᤊర♿ ✬㧕ޕRR ో 㗁

(3) ቇળ⊒⴫

Ԙ Shiraishi E., Miura, T., Abe, S. and Kitano T.

Role of Mullerian inhibiting substance on gonadal sex differentiation in medaka (Oryzias latipes). 8th International Symposium on Reproductive Physiology of Fish (St Malo, France), 3-8 June, 2007.

ԙ Shirozu, T., Shiraishi E., Yoshinaga N., Kanamori A., Kubo Y., Hori H. and Kitano T.

The mechanism of transcriptional regulation of Mullerian inhibiting substance in Japanese flounder (Paralichthys olivaceus) and medaka (Oryzias latipes). 8th International Symposium on Reproductive Physiology of Fish (St Malo, France), 3-8 June, 2007.

Ԛ Yamaguchi, T. and Kitano, T. The mechanism of transcriptional regulation of P450 aromatase gene by Foxl2 in Japanese flounder (Paralichthys olivaceus).

8th International Symposium on Reproductive Physiology of Fish (St Malo, France), 3-8 June, 2007

ԛ G,Mostafa,H.Ohgami,S.I.Abe and K.Takamune Xtr protein,One of the key Molecules for Progression of Meiotic Cycle in Xenopus laevis. 6th CONGRESS OF THE ASIA AND OCEANIA SOCIETY FOR COMPARATIVE ENDOCRINOLOGY, Dec. 10-14, Univ. of North Bengal, Siliguri, West Bengal, India

Ԝ J.Zhang,S.Okuma,K.Eto and S.-I.Abe Neuregulins Promote Mouse Spermatogonial Proliferation and Expression of Meiosis-Specific Genes in Vitro. 6th CONGRESS OF THE ASIA AND OCEANIA SOCIETY FOR COMPARATIVE ENDOCRINOLOGY, Dec.

10-14, Univ. of North Bengal, Siliguri, West Bengal, India

ԝ O.Oral,I.Uchida,K.Eto,H.Tarui,K.Agata,N.Araki,S-I.Abe Signaling Mechanism of Neuregulin1-dependent proliferation in Newt (Cynops pyrrhogaster) Spermatogonia. 6th CONGRESS OF THE ASIA AND OCEANIA SOCIETY FOR COMPARATIVE ENDOCRINOLOGY, Dec. 10-14, Univ. of North Bengal, Siliguri, West Bengal, India Ԟ K.Eto,K.Eda,M.Hayano,T.Kawasaki,H.Kasimura,K.Nagao,I.Toyooka,A.Utoguchi,H.Tarui,

O.Nishimura,K.Agata, and S.-I.Abe Transcriptional repression of a conserved RNA binding protein by prolactin leads to apoptosis in newt spermatogonia. 6th CONGRESS OF THE ASIA AND OCEANIA SOCIETY FOR COMPARATIVE ENDOCRINOLOGY, Dec. 10-14, Univ. of North Bengal, Siliguri, West Bengal, India

ԟ ᒛ⛮᧲ޔᄢ㓊⡛ሶޔᳯ㗡ᕡޔ቟ㇱ⋿৻ Neuregulins ߪࡑེ࠙ࠬቭၭ㙃ߦ߅޿ߡᷫ

ᢙಽⵚߩ㐿ᆎࠍଦㅴߔࠆޕ╙40࿁ᣣᧄ⊒↢↢‛ቇળ㧔⑔ጟ㧕

Ԡ Ko Eto, Kazufumi Eda, Motoshi Hayano, Toshihiro Kawasaki,Issei Toyooka,Ai Utoguchi, Shin-ichi Abe Protective role of a conserved RNA binding protein in spermatogonial apoptosis induced by prolactin during newt spermatogenesis.

╙40࿁ᣣᧄ⊒↢↢‛ቇળ㧔⑔ጟ㧕

ԡ ቟ㇱ⋿৻ޔᳯ㗡ᕡ ࠗࡕ࡝♖ේ⚦⢩ߦ߅ߌࠆᷫᢙಽⵚ㐿ᆎ߆ࠕࡐ࠻࡯ࠪࠬ߆ߩ ㆇ๮᳿ቯޕ╙78࿁ᣣᧄേ‛ቇળ㧔ᒄ೨㧕ࠪࡦࡐࠫ࠙ࡓ᜗ᓙ⻠Ṷ

Ԣ ቟ㇱᕺ␭ޔ቟ㇱ⋿৻ޔᳯ㗡ᕡ ࠗࡕ࡝♖Ꮍེቭၭ㙃ߦ߅ߌࠆ EGF ߩ♖ේ⚦⢩Ⴧᱺ ଦㅴലᨐޕ╙78࿁ᣣᧄേ‛ቇળ㧔ᒄ೨㧕

ԣ ᷓᶆ৻ᐘޔᳯ㗡ᕡޔ቟ㇱ⋿৻ ࠗࡕ࡝♖ේ⚦⢩ߩࠕࡐ࠻࡯ࠪࠬߦ߅ߌࠆ HSP90 ߣ ߩ⚿ว࠲ࡦࡄࠢ⾰ߩන㔌ޔหቯޕ╙32࿁ᣣᧄᲧセౝಽᴲቇળ㧔ᣣశ㧕

Ԥ ᪢ᧄዏ↵㧘቟ㇱ⋿৻㧘㊄↰ᾖᄦޟࠗࡕ࡝↢ᱺ⚦⢩ߩಽൻߦ෸߷ߔࡆࠬࡈࠚࡁ࡯࡞A ߩᓇ㗀ޠ╙13࿁ 㜞ኾࠪࡦࡐࠫ࠙ࡓ 㧔ਭ⇐☨㧕⻠Ṷⷐᣦ㓸 p28ޔ2008.1.26

ᐔᚑ ᐕᐲ⎇ⓥᚑᨐႎ๔

⎇ⓥ⺖㗴ฬ㧦㙃ᱺࡁ࡝⦡⪭ߜߦࠃࠆⅣႺࡕ࠾࠲࡝ࡦࠣ

ᚲዻ࡮᳁ฬ㧦ᴪጯၞⅣႺ⑼ቇᢎ⢒⎇ⓥ࠮ࡦ࠲࡯࡮Ṛየ ㅴ

⎇ⓥ⋡⊛࡮⋡ᮡ

* ᐕᐲ㨪 ᐕᐲߦ߅ߌࠆ⋡⊛࡮⋡ᮡ

ᬀ‛ߪᄖㇱⅣႺߩᄌൻߦኻߒߐ߹

ߑ߹ߥᔕ╵ࠍ␜ߔޕߥ߆ߢ߽㧘ࠛࡀ

࡞ࠡ࡯↢↥ߩ႐ߢ޽ࠆ⪲✛૕ߢߪ෻

ᔕਛᔃⶄว૕߿ࠕࡦ࠹࠽⦡⚛ⶄว૕

ߪశ᧦ઙߛߌߢߪߥߊᩕ㙃ᰳਲߥߤ ߩฦ⒳ࠬ࠻࡟ࠬߦ߽ᔕ╵ߒ࠳ࠗ࠽ࡒ

࠶ࠢߥᄌേࠍ␜ߔޕߒ߆ߒ㧘✛⦡ᬀ

‛ߢߪߘࠇࠄࠍ᭴ᚑߔࠆਥⷐߥ⦡⚛

߇ࠢࡠࡠࡈࠖ࡞ߢ޽ࠆߚ߼㧘ߎࠇࠄ ߩ᭴ᚑᚑಽߩ᭴ㅧᄌൻࠍᬀ‛ߩ⦡⺞

ᄌൻߣߒߡߪᬌ಴ߒߦߊ޿ޕ৻ᣇ㧘

⚃⮺߿࡜ࡦ⮺ߢߪ㧘ࠕࡦ࠹࠽⦡⚛ߣ ߒߡࠢࡠࡠࡈࠖ࡞ߩઁߦᄙ㊂ߩ⚃⦡

ࡈࠖࠦࡆ࡝ࡦ㘃ࠍ฽߻ߚ߼㧔࿑㧝㧕㧘ⅣႺᄌൻߦኻߒᄙ᭽ߥޟ⦡⺞ᄌൻޠࠍ␜ߔޕ

ࡁ࡝㙃ᱺߦ೑↪ߐࠇߡ޿ࠆ⚃⮺ࠬࠨࡆࡁ࡝ߪ㧘ᚒ࿖ߩᶏ᳓㕙㙃ᱺ↥ᬺߦ߅ߌࠆ㊀ⷐߥ⾗Ḯᬀ‛

ߩ߭ߣߟߢ޽ࠆޕ㙃ᱺࡁ࡝ߩ⦡⺞ߪ໡ຠଔ୯ࠍ᳿ቯߔࠆᦨ߽㊀ⷐߥᒻ⾰ߢ޽ࠅ㧘⿒ầߥߤߦࠃࠆ

޿ࠊࠁࠆޟࡁ࡝ߩ⦡⪭ߜޠߪ㙃ᱺ↥ᬺߦ߅޿ߡ㊀ⷐߥ⺖㗴ߩ৻ߟߢ޽ࠆ߇㧘㙃ᱺࡁ࡝ߩ⦡⺞ᒻᚑ ߩಽሶᯏ᭴ߪ߹ߞߚߊ⎇ⓥߐࠇߡ޿ߥ߆ߞߚޕᧄ⎇ⓥߩ⋡⊛ߪ㧘ᴪጯၞ᳓ⅣႺࡕ࠾࠲࡝ࡦࠣߩߚ

߼ߩᒻ⾰ォ឵ࠬࠨࡆࡁ࡝ࠍ૞಴ߔࠆ╙৻ᱠߣߒߡ㧘ࠬࠨࡆࡁ࡝ߩ⦡⺞ᒻᚑߦ㑐ਈߔࠆㆮવሶࠍಽ 㔌ߒ㧘ߘࠇࠄߩ⊒⃻⺞▵ᯏ᭴ࠍ᣿ࠄ߆ߦߔࠆߎߣߢ޽ࠆޕ

*ᐕᐲߩ⋡⊛࡮⋡ᮡ

;EHㆮવሶߪ࡜ࡦ⮺ߦ߅ߌࠆᩕ㙃ᰳਲߦࠃࠆ⦡⪭ߜࠍ೙ᓮߔࠆㆮવሶ0DN#ߣㆮવሶ㈩೉ߦ⋧

หᕈ߇ߺࠄࠇࠆߎߣ߆ࠄ㧘⎇ⓥᒰೋ߆ࠄߘߩ⊒⃻᭽ᑼࠍ⺞ߴߡ߈ߚޕ࡜ࡦ⮺0DN#߇ㅢᏱߩᩕ㙃⁁

ᘒߢߪ⊒⃻߇ᛥ೙ߐࠇ㧘ᩕ㙃ᰳਲߢ⊒⃻߇⺃ዉߐࠇࠆߩߦኻߒ㧘ࠬࠨࡆࡁ࡝;EHߪ⓸⚛ᰳਲߢ ߪ⺃ዉߐࠇߕ㧘⓸⚛Ḯࠍࠕࡦࡕ࠾ࠕߦಾࠅ឵߃ࠆߎߣߦࠃࠅ⊒⃻⺃ዉߐࠇࠆߎߣ߇ᤓᐕᐲߩ⎇ⓥ

ߦࠃࠅ᣿ࠄ߆ߦߥߞߚޕ

ࠬࠨࡆࡁ࡝ߪ㧘㘩↪ߣߒߡ೑↪ߐࠇߡ޿ࠆඨᢙ૕਎ઍߢߪ⪲⁁૕ߣߒߡጤߥߤߩၮ‛ߦઃ⌕ߒ ߡᚑ㐳ߒߡ޿ࠆ߇㧘୚ᢙ૕਎ઍߢߪ♻⁁૕ߣߥࠅ⽴Ზౝߦᶐ౉ߒᚑ㐳ߔࠆޕ↢ℂ↢ൻቇ⊛⎇ⓥߦ ߅޿ߡߪ㧘߅߽ߦ⪲⁁૕߇↪޿ࠄࠇߡ߅ࠅ㧘♻⁁૕ߩ↢ℂ⊛ᕈ⾰ߦߟ޿ߡߪਇ᣿ߥὐ߇ᄙ޿ޕᧄ

⎇ⓥߦ߅޿ߡ߽㧘ࠬࠨࡆࡁ࡝ߩⅣႺࡕ࠾࠲࡝ࡦࠣ߳ߩ೑↪ࠍ⋡⊛ߣߒߡ⪲⁁૕ࠍ↪޿ߡ⎇ⓥࠍㅴ

߼ߡ߈ߚޕ;EHߩࠕࡦࡕ࠾ࠕߦࠃࠆ⊒⃻⺃ዉ߽⪲⁁૕ࠍ↪޿ߡ᣿ࠄ߆ߦߥߞߚޕᧄᐕᐲߪ㧘⪲

⁁૕ߣߪోߊ⇣ߥࠆⅣႺߢᚑ⢒ߔࠆ♻⁁૕ߩ↢ℂ․ᕈࠍ᣿ࠄ߆ߦߔࠆ⋡⊛ߣߒߡ㧘ᩕ㙃ᰳਲߦࠃ ࠆ⦡⺞ᄌൻ߿ฦ⒳ࠬ࠻࡟ࠬਅߦ߅ߌࠆࡈࠖࠦࡆ࡝ࡦㆮવሶߣ;EHㆮવሶߩ⊒⃻ᔕ╵ߦߟ޿ߡ⺞

ߴߚޕ

ᐔᚑᐕᐲߩ⎇ⓥᚑᨐ

Ԙ♻⁁૕ߩ⓸⚛ᰳਲߦࠃࠆࡈࠖࠦࡆ࡝࠰࡯ࡓಽ⸃⢻

ᤓᐕ߹ߢߩ⎇ⓥߦࠃࠆߣ㧘⪲⁁૕ߪ⓸⚛ᰳਲ᧦ઙߢߪశวᚑ⦡⚛㊂߿ࡈࠖࠦࡆ࡝ࡦㆮવሶ 㧔ࡈࠖࠦࠛ࡝ࠬ࡝ࡦ%RG߿ࡈࠖࠦࠛ࡝ࠬ࡝ࡦ%REߥߤ㧕ߩ⊒⃻㊂߇ㅳ㑆એౝߦㅢᏱߩ⚂㧑એ ਅߦᷫዋߔࠆߎߣ߇᣿ࠄ߆ߦߥߞߡ޿ࠆޕ৻ᣇ㧘♻⁁૕ߢߪ⓸⚛ᰳਲߦ߅޿ߡ߽㗼⪺ߥㅌ⦡ߪ ߺࠄࠇߥ߆ߞߚ㧔࿑㧝#㧕ޕ♻⁁૕ߩ⓸⚛ᰳਲਅߢߩࡈࠖࠦࡆ࡝ࡦㆮવሶߩ⊒⃻㊂ࠍ⺞ߴߚߣߎ ࠈ㧘⺞ߴߚᦼ㑆ౝߢߪ㗼⪺ߥᄌൻߪ⷗ࠄࠇߥ߆ߞߚ㧔࿑㧝㧮㧕ޕᤓᐕ߹ߢߩ⎇ⓥ߆ࠄ㧘⪲⁁૕

ߦ߅޿ߡߪ;EHߩ⊒⃻ߪ⓸⚛ᰳਲߦࠃࠅ⺃ዉߐࠇߥ߆ߞߚޕ♻⁁૕ߦ߅޿ߡ߽⪲⁁૕ߣห᭽

ߦ㧘⓸⚛ᰳਲߦࠃߞߡߪ;EHߩ⊒⃻ߪ⺃ዉߐࠇߥ߆ߞߚޕ

࿑ ή⓸⚛ၭ㙃ߦ઻߁శวᚑ⦡⚛㊂㧔#㧕ߣ⪲✛૕㑐ㅪㆮવሶߩ⊒⃻㊂㧔$㧕ߩᄌൻ

㧔#㧕ㅢᏱߩၭ㙃ᶧߢ⛮ઍၭ㙃ߐࠇߚ⪲⁁૕߅ࠃ߮♻⁁૕ࠍή⓸⚛ၭ࿾ߦ⒖ߒၭ㙃ࠍⴕ޿㧘

ੇ῎㊀㊂޽ߚࠅߩฦ⦡⚛㊂ࠍ᷹ቯߒߚޕ$♻⁁૕ࠍㅢᏱߩ⎣㉄Ⴎၭ࿾߆ࠄή⓸⚛ၭ࿾ߦ⒖ߒ㧘 ฦᣣᢙߢో 40# ࠍ᛽಴ߒ㧘࡜ࡦ࠳ࡓࡊ࡜ࠗࡑ࡯ߦࠃࠅ㨏&0# ࠍวᚑߒ㧘;EH㧘ࡈࠖࠦࠛ࡝ࠬ࡝

ࡦ㧔%RG㧕㧘ࡈࠖࠦࠪࠕ࠾ࡦ%RE߅ࠃ߮⠡⸶િ㐳࿃ሶ㧔'(C㧕ߩฦㆮવሶ↪ࡊ࡜ࠗࡑ࡯ߦࠃࠅ 2%4 ࠍⴕߞߚޕ

ԙ ♻⁁૕ߩ㌃ࠬ࠻࡟ࠬߦኻߔࠆᔕ╵

⪲⁁૕ߦᲧߴ♻⁁૕ߪ㧘⓸⚛ᰳਲਅ ߅ߌࠆࡈࠖࠦࡆ࡝࠰࡯ࡓಽ⸃⢻߇⪺ߒ ߊૐ޿ߎߣ߇᣿ࠄ߆ߦߥߞߚޕߘߩઁ

ߩࠬ࠻࡟ࠬߦኻߔࠆᔕ╵ߣߒߡ㌃ㆊ೾

ᷝട㧔Ǵ/ ⎫㉄㌃㧕ߦࠃࠆ㊀㊄ዻ

ࠬ࠻࡟ࠬߦߟ޿ߡ⺞ߴߚ㧔࿑㧞㧕ޕ⓸

⚛ᰳਲߣߪ⇣ߥࠅ㧘♻⁁૕ߪ⪲⁁૕ߣ ห᭽ߦ㌃ࠬ࠻࡟ࠬߦኻߒߡ⚛ᣧߊᔕ╵

ߔࠆߎߣ߇᣿ࠄ߆ߦߥߞߚޕ

ࣈƒ Ѕࡶସ૘ϧǸȕȘ৤࢑

Ԛ ;EH ㆮવሶߩࠕࡦࡕ࠾ࠕߦࠃࠆ⊒⃻⺃ዉ

♻⁁૕ߦ߅ߌࠆࠕࡦࡕ࠾ࠕၭ㙃ߩᓇ㗀ࠍㆮવሶ⊒⃻߅ࠃ߮⦡⚛วᚑߦߟ޿ߡ⺞ߴߚ㧔࿑㧟#㧕ޕ

⎣㉄Ⴎࠍ໑৻ߩ⓸⚛ḮߣߔࠆㅢᏱၭ࿾ߢߪ ;EH ߩ⊒⃻ߪ⪺ߒߊૐ޿߇㧘⓸⚛Ḯࠍࠕࡦࡕ࠾ࠕ ߦಾࠅ឵߃ࠆߣ ;EH ߩ⊒⃻߇⺃ዉߐࠇߚޕ৻ᣇ㧘ࡈࠖࠦࠛ࡝ࠬ࡝ࡦ߿ࡈࠖࠦࠪࠕ࠾ࡦㆮવሶ ߩ⊒⃻㊂ߪ㗼⪺ߥᄌൻߪߺࠄࠇߕ㧘߹ߚ㧘శวᚑ⦡⚛㊂߽ᄌൻ߇ߺࠄࠇߥ߆ߞߚޕ;EH ߩ⊒

⃻ߦ᦭ലߥࠕࡦࡕ࠾࠙ࡓỚᐲߪ O/ ߢ޽ߞߚ㧔࿑㧟$㧕ޕࠕࡦࡕ࠾࠙ࡓ O/ ߢߪᚑ㐳㒖ኂ߇⷗

ࠄࠇߚޕ

ԛ ♻⁁૕߅ࠃ߮⪲⁁૕ߩࠕࡦࡕ࠾ࠕหൻ♽㉂⚛ㆮવሶߩ⊒⃻࡟ࡌ࡞

;EH ߩ⊒⃻߇ࠕࡦࡕ࠾ࠕߦࠃࠅ⺃

ዉߐࠇࠆߎߣ߇㧘♻⁁૕ߢ߽⏕⹺ߐࠇ ߚޕߎߩ⃻⽎ߣࠕࡦࡕ࠾ࠕหൻ♽ߣߩ 㑐ㅪߦߟ޿ߡ᣿ࠄ߆ߦߔࠆᔅⷐ߇޽

ࠆߣ⠨߃ࠄࠇߚޕࠕࡦࡕ࠾ࠕหൻ♽㉂

⚛߿ߘߩ⺞▵ᯏ᭴ߦߟ޿ߡߪ㧘ⵍሶᬀ

‛߿৻ㇱߩ✛⮺ߢߪ⎇ⓥ߇ㅴࠎߢ޿

ࠆ߇㧘ࠬࠨࡆࡁ࡝ࠍ฽߻ᄢဳᶏ⮺ߢߪ

߶ߣࠎߤ⎇ⓥߐࠇߡ޿ߥ߆ߞߚޕోႮ ၮ㈩೉߇᳿ቯߐࠇߡ޿ࠆࠬࠨࡆࡁ࡝

⪲✛૕ࠥࡁࡓߦߪ⓸⚛ઍ⻢♽㉂⚛ㆮ વ ሶ ߣ ߒ ߡ ࠣ ࡞ ࠲ ࡒ ࡦ ↢ ᚑ ㉂ ⚛ 㧔)1)#6㧕߇৻ߟࠦ࡯࠼ߐࠇߡ޿ߚޕ

߹ߚ㧘ࠬࠨࡆࡁ࡝ '56 ࠺࡯࠲࡯ࡌ࡯ࠬ

ߦߪࠣ࡞࠲ࡒࡦวᚑ㉂⚛㧔)5㧕ߣࠣ࡞

࠲ࡒࡦ㉄⣕᳓⚛㉂⚛㧔)&*㧕ߩࠦࡦ࠹ࠖࠣ߇ฦ৻⒳㘃⷗಴ߖߚޕߎࠇࠄߩㆮવሶߩ⊒⃻ࠍ⺞ߴߚ ߣߎࠈ㧘)&* ߩ⊒⃻࡟ࡌ࡞ߪૐߊ㧘ࠕࡦࡕ࠾ࠕߦࠃߞߡ߽⊒⃻ߪᄌൻߒߥ߆ߞߚ㧔࿑㧠㧕ޕ৻ᣇ㧘

ࣈ1 ȢɳɢɋȢǸȕȘWad/6ǻ௧ؕහଯ

&?'ȢɳɢɋȦɠஷ෪ǸǗǞȘஷ෪୸ࣞǸ௴Ǔ

͌૟݄௧ؕĦࡱħǵ࢑७ุǻ೅ϡĦϠħ

&@'ȢɳɢɋȦɠக૷Ǹ̵়ǤǬWad/6ǻ௧ؕ

ĦAħ ĦBħ

ࣈƔ ȢɳɢɋȢଭϡ׉͌૟݄ǻ௧ؕɬəɫ

&?'ȢɳɢɋȢଭϡ׉ǻൿހࣈ &@'ࡗܲμǗȕȀȢ ɳɢɋȦɠμஷ෪ǸǗǞȘȢɳɢɋȢଭϡ׉͌૟݄

ǻ௧ุؕ

(A) (B)

)5 ߣ )1)#6 ߩ⊒⃻㊂ߪ㜞ߊ㧘)1)#6 ߪࠕࡦࡕ࠾ࠕߦࠃࠅ⊒⃻߇Ⴧᄢߒߚޕߎࠇࠄߩ⚿ᨐ߆ࠄ㧘ࠕ ࡦࡕ࠾ࠕၭ࿾ߦ߅ߌࠆࠕࡦࡕ࠾ࠕหൻߩਥⷐߥ⚻〝ߪ )5)1)#6 ࿁〝ߢ޽ࠆߣផቯߐࠇߚޕ㜞╬

ᬀ‛ߢߪ㧘)5 ߦߪ⚦⢩⾰ዪ࿷ߣ⪲✛૕ዪ࿷ߩ ⒳ߩࠗ࠰㉂⚛߇ሽ࿷ߒ㧘)1)#6 ߢߪഥ㉂⚛ߣߒߡ ㆶరဳࡈࠚ࡟࠼ࠠࠪࡦߣ 0#&2* ࠍ೑↪ߔࠆ ⒳ߩࠗ࠰㉂⚛߇ሽ࿷ߔࠆߎߣ߇⍮ࠄࠇߡ޿ࠆޕࠬࠨ ࡆࡁ࡝ߦ߅޿ߡ㧘ࠕࡦࡕ࠾ࠕߦࠃࠅჇᄢߒߚ )1)#6 ߿ )5 ㆮવሶߩ⊒⃻߇⎣㉄ၭ㙃ߣߪ⇣ߥࠆࠗ

࠰㉂⚛ㆮવሶߦ↱᧪ߔࠆน⢻ᕈ߇ᱷߐࠇߡ޿ࠆޕࠕࡦࡕ࠾ࠕၭ㙃ߦ߅ߌࠆ 2%4 ↥‛ߩࠪ࡯ࠤࡦࠬ

ߦࠃࠅߎߩ໧㗴ࠍᬌ⸛ߔࠆᔅⷐ߇޽ࠆޕ

ᚑᨐߩ㆐ᚑᐲ

;EHߩࠕࡦࡕ࠾ࠕߦࠃࠆ⊒⃻⺃ዉ߇⪲⁁૕ߛߌߢߥߊ♻⁁૕ߦ߅޿ߡ߽⏕⹺ߢ߈ߚޕ߹ߚ㧘

㌃ࠬ࠻࡟ࠬߦኻߒߡ߽♻⁁૕ߪ⪲⁁૕ߣห᭽ߦ⦡⺞ᄌൻࠍ␜ߔߩߦኻߒ㧘⓸⚛ᰳਲࠬ࠻࡟ࠬߦኻ ߒߡߪ♻⁁૕ߪ⠴ᕈࠍ߽ߟߎߣ߇᣿ࠄ߆ߦߥߞߚޕߎߩࠃ߁ߦ㧘♻⁁૕ߢߪ⪲⁁૕ߣߪ⇣ߥࠆࠬ

࠻࡟ࠬᔕ╵ࠍ߽ߟߎߣ߇᣿ࠄ߆ߦߥࠅ㧘♻⁁૕ߢߩࠬ࠻࡟ࠬᔕ╵․ᕈࠍ᣿ࠄ߆ߦߔࠆߣ޿߁ᧄᐕ ᐲߩ⋡ᮡߪචಽ㆐ᚑߐࠇߚߣ⠨߃ࠄࠇࠆޕ

੹ᐕᐲߩ໧㗴ߥߤ ․ߦߥߒޕ

᧪ᐕᐲߩ⋡⊛࡮⋡ᮡ

㜞╬ᬀ‛ߢߪ㧘)1)#6 ߩၮ⾰ߢ޽ࠆ㧞гࠝࠠ࠰ࠣ࡞࠲࡞㉄ߪ 6%# ࿁〝߆ࠄଏ⛎ߐࠇࠆߎߣ߆ࠄ㧘 㧞гࠝࠠ࠰ࠣ࡞࠲࡞㉄ߩ⚦⢩ౝỚᐲߦࠃߞߡ⓸⚛ઍ⻢♽ߣ὇⚛ઍ⻢♽߇೙ᓮߐࠇߡ޿ࠆޕ⚃⮺㘃 ߦ߅޿ߡߪߘࠇࠄߩ೙ᓮᯏ᭴ߪਇ᣿ߢ޽ࠆޕ߹ߕ㧘)5)1)#6 ࿁〝ߩ㒖ኂ೷߿㧞гࠝࠠ࠰ࠣ࡞࠲࡞

㉄ߥߤߩࠕࡦࡕ࠾ࠕหൻ♽ߦ㑐ਈߔࠆࠕࡒࡁ㉄ߩᷝടലᨐࠍ⺞ߴ㧘ࠕࡦࡕ࠾ࠕหൻ♽ߩ೙ᓮ᭽ᑼ ࠍ᣿ࠄ߆ߦߔࠆᔅⷐ߇޽ࠆޕߎߩ⎇ⓥߦࠃࠅ㧘;EH ߩ⺃ዉ߇ࠕࡦࡕ࠾ࠕߦࠃࠅ⋥ធ೙ᓮߐࠇߡ

޿ࠆߩ߆㧘ࠕࡦࡕ࠾ࠕหൻ♽ߩઍ⻢↥‛ߦࠃࠅ೙ᓮߐࠇߡ޿ࠆߩ߆߇᣿ࠄ߆ߥࠆߣ⠨߃ࠄࠇࠆޕ ⎇ⓥᒰೋߪ ;EH ߇ᩕ㙃ᰳਲߦࠃࠆ⦡⺞ᄌൻ㧔ࡁ࡝ߩ⦡⪭ߜ㧕ࠍ೙ᓮߔࠆࡑࠬ࠲࡯ㆮવሶߣផ ቯߒߡ޿ߚ߇㧘⃻ᤨὐߢߪ㧘⦡⺞ᄌൻߣߪ㑐ㅪߒߥ޿น⢻ᕈ߇㜞ߊߥߞߚޕߒ߆ߒ㧘ᤓᐕ㧘࡜ࡦ

⮺ߩ⓸⚛ᰳਲߦࠃࠆ⦡⪭ߜࠍ೙ᓮߔࠆㆮવሶߣߒߡࠕ࡜࠾ࡦ⣕᳓⚛㉂⚛߇หቯߐࠇߚޕหㆮવሶ ࠍ⎕უߔࠆߣ㧘⓸⚛ᰳਲਅߢ߽ㅌ⦡߇⷗ࠄࠇߥߊߥࠆޕ੹ߩߣߎࠈ㧘⓸⚛ᰳਲߦ߅ߌࠆࡈࠖࠦࡆ

࡝࠰࡯ࡓಽ⸃ߦห㉂⚛߇ߤߩࠃ߁ߦ㑐ਈߒߡ޿ࠆ߆ߪోߊਇ᣿ߢ޽ࠆޕߒ߆ߒ㧘ห㉂⚛ߪ෻ᔕ↥

‛ߣߒߡࠕࡦࡕ࠾ࠕࠍ↢ᚑߔࠆߎߣ߆ࠄ㧘⓸⚛ᰳਲߦࠃࠆࡈࠖࠦࡆ࡝࠰࡯ࡓಽ⸃ㆊ⒟ߦ߅޿ߡ⓸

⚛ߩౣ೑↪ߩߚ߼ߦࠕࡦࡕ࠾ࠕหൻ♽߇ᵴᕈൻߐࠆน⢻ᕈ߇⠨߃ࠄࠇࠆޕ;EH ߇ࠕࡦࡕ࠾ࠕߦ ࠃࠅ⺃ዉߐࠇࠆ⃻⽎ߪߎߩὐߢ⥝๧ᷓߊ㧘;EH ߇ࡈࠖࠦࡆ࡝࠰࡯ࡓߩวᚑ࡮ಽ⸃ߦ㑆ធ⊛ߦ㑐 ਈߔࠆน⢻ᕈߪᱷߐࠇߡ޿ࠆߣ⠨߃ࠄࠇࠆޕ

;EH ߩ⪲✛૕ౝߢߩዪ࿷ㇱ૏߽ߘߩᯏ⢻ࠍ⠨߃ࠆ਄ߢ㊀ⷐߥ໧㗴ߢ޽ࠆޕㄭᐕ㧘࡜ࡦ⮺ 0DN#

ߪࡈࠖࠦࠪࠕ࠾ࡦ࠲ࡦࡄࠢߣ⚿วߔࠆߎߣ߇␜ߐࠇߡ޿ࠆޕ৻ᣇ㧘㜞╬ᬀ‛ߢߪ㧘⪲✛૕ౝߦ߅ ߌࠆࠕࡦࡕ࠾ࠕหൻ♽㉂⚛ߪߔߴߡนṁᕈᚑಽߢ޽ࠆࠬ࠻ࡠࡑߦዪ࿷ߒߡ޿ࠆߎߣ߇⍮ࠄࠇߡ

޿ࠆޕએ೨ߩ⎇ⓥߢߪࠬࠨࡆࡁ࡝ ;EH ߪ࠴࡜ࠦࠗ࠼⤑ߦ⚿วߒߡ޿ࠆߣ޿߁⚿ᨐ߇ᓧࠄࠇߡ޿

ߚޕߒ߆ߒ㧘ߎߩᤨߦ૶↪ߒߚ᧚ᢱߪㅢᏱ᧦ઙߢၭ㙃ߐࠇߚ⪲⁁૕ߢ޽ߞߚޕ੹ᓟߪࠕࡦࡕ࠾ࠕ

ၭ㙃ߩ᧚ᢱࠍ↪޿ߡ ;EH ߩ⪲✛૕ౝߢߩዪ࿷ㇱ૏ࠍ᣿ࠄ߆ߦߔࠆᔅⷐ߇޽ࠆޕ

㧚ᐔᚑᐕᐲߩ⎇ⓥ⾌ขᓧ⁁ᴫ

ၮ⋚⎇ⓥ㧯 ਁ౞ᐔᚑᐕᐲಽ࡟࠻ࡠ࠻࡜ࡦࠬࡐ࠱ࡦߦࠃࠆࠬࠨࡆࡁ࡝ಽሶ⢒⒳ᴺ ߩ㐿⊒

㧚ᐔᚑᐕᐲߩ⎇ⓥ⊒⴫ޔ․⸵⁁ᴫ

ේ⪺⺰ᢥߦࠃࠆ⊒⴫

Ԙ ࿖ౝ⹹㧔๺ᢥ㧕 ߥߒ

ԙ࿖㓙⹹㧔᰷ᢥ㧕

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ේ⪺⺰ᢥએᄖߦࠃࠆ⊒⴫

ߥߒ

ญ㗡⊒⴫

ᱞᥓసᓆޔ଻⷗␽ሶ㧘㜞ᯅ⦟ሶ㧘૒⮮ඳ㧘Ṛየㅴޔ㜞㊁ඳཅࡅࡔ࠷࡝ࠟࡀࠧࠤ⪲✛૕ߦ߅ ߌࠆࡍࡊ࠴࠼ࠣ࡝ࠞࡦวᚑ♽ߣ⪲✛૕ಽⵚ╙ ࿁ᣣᧄᬀ‛ቇળ਻Ꮊᡰㇱᄢળ ᐕ ᦬ — ᣣ

᧲૓ᒎޔᱞᥓసᓆޔ㜞㊁ඳཅޔṚየㅴࡅࡔ࠷࡝ࠟࡀࠧࠤᏂᄢ⪲✛૕ᩣߩࠬ࠻࡟ࠬ㒐ᓮ⢻ߩ

⸃ᨆ╙ ࿁ᣣᧄᬀ‛ቇળ਻Ꮊᡰㇱᄢળ ᐕ ᦬ — ᣣ 6CMKQ5<JCPI92GFFKICTK56CMGEJK-CPF6CMCPQ*

4GVTQVTCPURQUQPTGNCVGFUGSWGPEGUKPCTGFCNIC2QTRJ[TC[G\QGPUKU9QTMUJQR4GEGPV RTQITGUUKPOCTKPGDKQNQIQ[CPFDKQVGEJPQNQI[KP-QTGCCPF,CRCP╙ ࿁ᣣᧄࡑ࡝ࡦ ࡃࠗࠝ࠹ࠢࡁࡠࠫ࡯ቇળ ᐕ ᦬ г ᣣ

᧲૓ᒎޔᱞᥓసᓆޔ㜞㊁ඳཅޔṚየㅴῺޔࡅࡔ࠷࡝ࠟࡀࠧࠤᏂᄢ⪲✛૕ᩣߩࠬ࠻࡟ࠬ㒐ᓮ⢻

ߩ⸃ᨆᣣᧄᬀ‛ቇળ╙ ࿁ᄢળ ᐕ ᦬ — ᣣ

ᱞᥓసᓆޔ଻⷗␽ሶ㧘㜞ᯅ⦟ሶ㧘૒⮮ඳ㧘Ṛየㅴޔ㜞㊁ඳཅࡅࡔ࠷࡝ࠟࡀࠧࠤ⪲✛૕ಽⵚ ߦ㑐ਈߔࠆࡍࡊ࠴࠼ࠣ࡝ࠞࡦวᚑ♽ 2RDR ߩᯏ⢻⸃ᨆᣣᧄᬀ‛ቇળ╙ ࿁ᄢળ ᐕ

᦬ — ᣣ

㍿ፉ৻⌀㧘/CTNQP)CTEKC᣿⾐ผ⚐㧘ᱞᥓసᓆޔ૒⮮ඳ㧘᳗↰ౖሶ㧘Ṛየㅴޔ◉ፒ৻㓶㧘㜞

㊁ඳཅ⪲✛૕ᒻᚑਇోࠍ␜ߔࠪࡠࠗ࠿࠽࠭࠽ /WT' ᄌ⇣૕ߩ⦡⚛૕᭴ㅧᣣᧄᬀ‛ቇળ╙

࿁ᄢળ ᐕ ᦬ — ᣣ!

!

ᐔᚑ ᐕᐲ⎇ⓥᚑᨐႎ๔

⎇ⓥ⺖㗴ฬ㧦ⅣႺᳪᨴ‛⾰ߩࡃࠗࠝ࡮࡟ࡔ࠺ࠗࠛ࡯࡚ࠪࡦ

ᚲዻ࡮᳁ฬ㧦ᄢቇ㒮⥄ὼ⑼ቇ⎇ⓥ⑼࡮ฎᎹᙗᴦ

㧝㧚 ⎇ⓥ⋡⊛࡮⋡ᮡ

㧨ᐔᚑ ᐕᐲ㨪 ᐕᐲߦ߅ߌࠆ⋡⊛࡮⋡ᮡ㧪

㐽㎮ᕈᐲߩ⪺ߒߊ㜞޿᦭᣿࡮౎ઍᶏߩንᩕ㙃ൻߩㅴⴕ߇ᄢ߈ߥ␠ળ໧㗴ߣߥ ߞߡ޿ࠆޕ⓸⚛ޔ࡝ࡦߩᩕ㙃Ⴎ߇᳓ၞንᩕ㙃ൻߩේ࿃‛⾰ߢ޽ࠆ߇ޔߘߩᳪᨴ ḮߣߒߡߪᎿ႐ឃ᳓ߣ߆ਅ᳓ಣℂ႐╬ߩࡐࠗࡦ࠻࠰࡯ࠬߣㄘ࿾╬߆ࠄߩࡁࡦࡐ

ࠗࡦ࠻࠰࡯ࠬ߇᜼ߍࠄࠇࠆޕࡐࠗࡦ࠻࠰࡯ࠬߦߪޔ᳓ၞⅣႺၮḰࠍ㆐ᚑߔࠆߚ

߼ߦޔ෩ߒ޿ឃ᳓ၮḰ㧔60OINޔ62OIN㧕߇᳓⾰ᳪỘ㒐ᱛᴺߦၮߠ޿ߡ ߆ߌࠄࠇࠆ߽ߩߣ੍ᗐߐࠇߡ޿ࠆޕ

ឃ᳓߆ࠄߩ⓸⚛㒰෰ᴺߦ㑐ߒߡߪޔߎࠇ߹ߢ↢‛ቇ⊛ߥ⎣ൻޔ⣕⓸෻ᔕࠍ⚵

ߺวࠊߖߚಣℂᣇᴺ߇ㆡ↪ߐࠇߡ߈ߚ߇ޔߘߩታᣉߦߪᏂᄢߥಣℂࠪࠬ࠹ࡓ߇ ᔅⷐߣߥࠆ߫߆ࠅ߆ޔಣℂࠦࠬ࠻߽ᓥ᧪ߩ $1&ޔ55 㒰෰ࠍ⋡⊛ߣߒߚ ᰴಣℂ ߩ⚂ ୚ߦ߽ߥࠆߎߣ߆ࠄޔല₸⊛ޔ⚻ᷣ⊛ߥᩕ㙃Ⴎ㒰෰ᣇᴺߩ㐿⊒߇ᦸ߹

ࠇߡ޿ࠆޕ

ᣂߒ޿⓸⚛ߩઍ⻢⚻〝ߢ޽ࠆህ᳇ᕈࠕࡦࡕ࠾ࠕ㉄ൻ#PCGTQDKECOOQPKWO QZKFCVKQP#PCOOQZߪޔߎࠇ߹ߢߩ⓸⚛㒰෰ߩ᭎ᔨࠍᄢ߈ߊᄌ߃ࠆ߽ߩߢޔᄙ ߊߩ⎇ⓥ⠪ߩᵈ⋡ࠍ㓸߼ߡ޿ࠆޕฎᎹ⎇ⓥቶߢߪޔ ᐕ߆ࠄߎߩ #PCOOQZ ⚦

⩶ߩ㓸Ⓧߦ㑐ߔࠆ⎇ⓥࠍ㐿ᆎߒޔࡐ࡝ࠛࠬ࠹࡞⵾ਇ❱Ꮣࠍᓸ↢‛ߩઃ⌕ᜂ૕ߣ ߒߡᵴ↪ߒߚ਄ะᵹࠞ࡜ࡓ࡝ࠕࠢ࠲ࠍ↪޿ߡᣣᧄߢᆎ߼ߡޔ਎⇇ߢ߽㧟⇟⋡ߦ

#PCOOQZ ᳪᵆߩ㓸Ⓧၭ㙃ߦᚑഞߒߚޕ⃻࿷ #PCOOQZ ࠍᵴ↪ߒߚឃ᳓ಣℂࠪࠬ࠹ࡓ ߩ⏕┙ߦะߌߡ . ߩኈⓍߩᄢဳ࡝ࠕࠢ࠲ࠍᵴ↪ߒߡ #PCOOQZ ᳪᵆߩᄢ㊂ၭ㙃 ߦ㑐ߔࠆ⎇ⓥࠍታᣉߒߡ޿ࠆޕ

ᐔᚑ ᐕᐲ㨪 ᐕᐲߩ⎇ⓥᦼ㑆ਛߢߪޔߎߩ㓸Ⓧߦᚑഞߒߚ #PCOOQZ ᳪᵆࠍ ᵴ↪ߒߡޔല₸⊛ߥ⓸⚛㒰෰ᴺࠍ㐿⊒ߔࠆߎߣࠍ⋡⊛ߣߒߡ⎇ⓥࠍⴕ߁ޕ

ᐔᚑ ᐕᐲ߹ߢߦᓧࠄࠇߚ #PCOOQZ ᳪᵆߩ㓸Ⓧ߿ㇱಽ੝⎣㉄ൻಣℂߦ㑐ߔࠆ

⎇ⓥቶߢߩ⎇ⓥᚑᨐࠍၮߦߒߡޔᐔᚑ ᐕᐲߪᰴߩ⋡⊛࡮⋡ᮡߢ⎇ⓥࠍⴕ߁ޕ ࡃࠗࠝࠟࠬࡊ࡜ࡦ࠻⣕㔌ᶧࠍಣℂኻ⽎ߣߔࠆ㧔ㇱಽ੝⎣㉄ൻ㧗#PCOOQZ㧕ಣ

ℂߩࡌࡦ࠴ࠬࠤ࡯࡞⹜㛎ࠍⴕ޿ޔ$1&ޔ55 Ớᐲߩ㜞޿ឃ᳓߳ߩ #PCOOQZ ෻ᔕ ߩㆡ↪ߩน⢻ᕈࠍ᣿ࠄ߆ߦߔࠆޕ

ㇺᏒਅ᳓ಣℂ႐ߩህ᳇ᕈᶖൻ⣕㔌ᶧࠍಣℂኻ⽎ឃ᳓ߣߔࠆࡌࡦ࠴ࠬࠤ࡯࡞

⹜㛎ࠍⴕ޿ޔ#PCOOQZ ෻ᔕߩㆡ↪ߩน⢻ᕈࠍ᣿ࠄ߆ߦߔࠆޕ

㧞㧚 ⎇ⓥᣇᴺ

ࡃࠗࠝࠟࠬࡊ࡜ࡦ࠻⣕㔌ᶧߩㇱಽ੝⎣㉄ൻಣℂࠍࡐ࡝ࠛ࠴࡟ࡦࠣ࡝ࠦ࡯࡞

2')ߢ൮᜝࿕ቯൻߒߚ⎣ൻࡍ࡟࠶࠻ࠍ↪޿ߡⴕ߁ޕ

⎣ൻࡍ࡟࠶࠻ߦࠃࠆㇱಽ੝⎣㉄ൻಣℂ᳓ࠍޔࠣ࡜࠾ࡘ࡯࡞⁁ߩ #PCOOQZ ᳪᵆ ࠍᵴ↪ߔࠆ #PCOOQZ ࡝ࠕࠢ࠲ߢಣℂߒޔߘߩಣℂ⢻ജࠍ᣿ࠄ߆ߦߔࠆޕ ㇺᏒਅ᳓ಣℂ႐ߩህ᳇ᕈᶖൻ⣕㔌ᶧࠍಣℂኻ⽎ឃ᳓ߣߒߡޔࡃࠗࠝࡈ࡝ࡦࠫ

$(ࠍᵴ↪ߔࠆំേᐥߢㇱಽ੝⎣㉄ൻಣℂߔࠆޕᓧࠄࠇߚㇱಽ੝⎣㉄ൻಣℂ

᳓ࠍޔ✂⋡⁁ߩࠕࠢ࡝࡞⵾ᜂ૕㧔ࡃࠗࠝࡈࠖ࠶ࠢࠬ$:㧕ࠍᜂ૕ߣߔࠆኈ㊂ . ߩ #PCOOQZ ࡝ࠕࠢ࠲ߢಣℂߒޔߘߩಣℂ⢻ജࠍ᣿ࠄ߆ߦߔࠆޕ

㧟㧚 ⎇ⓥᚑᨐ

㧝㧕

Ԙ . ኈߩ #PCOOQZ ࡝ࠕࠢ࠲ߩㄦㅦ┙਄ߍ⹜㛎ࠍⴕ޿ޔ ᣣߢ 60 ኈⓍ㒰෰ㅦ ᐲ MI0O

F ࠍޔ ᣣᓟߦ MI0O

F ࠍ㆐ᚑߔࠆߎߣ߇ߢ߈ߚޕ ԙ 28# ࠥ࡞ࡆ࡯࠭ࠍᜂ૕ߣߔࠆ #PCOOQZ ࡝ࠕࠢ࠲ߩ᭴▽ߦᚑഞߒޔ60 ኈⓍ㒰

෰ㅦᐲ MI0O

F ࠍ㆐ᚑߢ߈ߚޕ

Ԛ #PCOOQZ ߩᜂ૕ߣߒߡޔࡕ࡞࠻࠮࡜ࡒ࠶ࠢࠬޔࡐ࡝ࠛ࠴࡟ࡦࠬࡐࡦ߽ࠫᵴ↪

ߢ߈ࠆߎߣ߇ㅪ⛯ಣℂ⹜㛎ߢ⏕⹺ߢ߈ߚޕ

ԛ ৻ᮏᑼߩࠕࡦࡕ࠾ࠕ㒰෰ᴺ 50#2 ߩ㐳ᦼ⊛ߥಣℂߩ቟ቯᕈࠍޔวᚑၒ┙࿾ᶐ

಴᳓ࠍ૶ߞߚㅪ⛯⹜㛎ߢᬌ⸛ߒޔ60 ኈⓍ㒰෰ㅦᐲ MI0O

F ࠍ቟ቯߒߡ

⛽ᜬߢ߈ࠆߎߣࠍ᣿ࠄ߆ߦߒߚޕ

㧞㧕

Ԙ 2') ߢ൮᜝࿕ቯൻߒߚ⎣ൻࡍ࡟࠶࠻ࠍᵴ↪ߔࠆࡃࠗࠝࠟࠬࡊ࡜ࡦ࠻⣕㔌ᶧߩ ㇱಽ੝⎣㉄ൻಣℂ

࿑㧝ߦ␜ߔኈⓍ . ߩࠕࠢ࡝࡞⵾ࠞ࡜ࡓ࡝ࠕࠢ࠲ࠍ↪޿ߡⴕߞߚޕଏ⹜ᜂ૕ߣ ߒߡ⎣ൻᳪᵆࠍ൮᜝࿕ቯߒߚ⎣ൻࡍ࡟࠶࠻ࠍ లႯߒߚޕ⚂ TRO ߩᠣᜈㅦ ᐲߢᠣᜈߒޔࠛࠕࡐࡦࡊߢ㑆ᰳᦑ᳇ࠍⴕ޿ޔᜂ૕ߣᵹ౉᳓ࠍᷙวߐߖߚޕ&1 ࠍ 㨪OI. ߩ▸࿐ߢࠦࡦ࠻ࡠ࡯࡞ߔࠆߎߣߦࠃࠅޔ㑆ᰳᦑ᳇ࠍⴕߞߚޕ⓸⚛

⽶⩄㊂ࠍ਄᣹ߐߖࠆߦߟࠇߡޔ⸳ቯ &1 Ớᐲࠍ਄᣹ߐߖߚޕR* ߪ R* ࠦࡦ࠻ࡠ࡯

࡜ࠍ↪޿ߡ 㨪 ߦ⸳ቯߒޔಣℂ᷷ᐲߪ࠙ࠜ࡯࠲࡯ࠫࡖࠤ࠶࠻ࠍ↪޿ߡ ͠ ߦ⛽ᜬߒߚޕR* ⺞ᢛᶧߦߪ 0C*%1

㧔I.㧕ߣ *%N㧔0㧕ࠍ૶↪ߒߚޕଏ⹜ᑄ᳓

ߪ⓸⚛⽶⩄㊂ߦวࠊߖߡ᳓㆏᳓ߢᏗ㉼ߒޔᵹ౉᳓ߣߒߚޕ߹ߚޔ⊒ᵃ⃻⽎߇↢

ߓߚ႐วߪޔᶖᵃ೷㧔ା⿧ࠪ࡝ࠦ࡯ࡦ -/㧕ࠍㆡቱᛩ౉ߒߚޕήᏗ㉼ේ᳓ߩ

ᵹ౉᳓ࠍ↪޿ᆎ߼ߡ߆ࠄߪޔᵹ౉᳓ਛߩ 55 Ớᐲ߇ 㨪OI. ߣ㜞߹ߞߚ ߚ߼ߦޔᢔ᳇▤ߩ⋡⹣߹ࠅߦᵈᗧߒޔ⋡⹣߹ࠅߒߚ㓙ߦߪㆡቱ੤឵ߒߚޕ

࿑㧝 ታ㛎ⵝ⟎࿑

9CVGTLCEMGV*%NUQNWVKQP0C*%1UQNWVKQPR*EQPVTQNNGT

&1EQPVTQNNGT5VKTTGT#KTRWOR+PHNWGPV'HHNWGPV

࿑ 㧞 ߦ ฦ ᘒ ⓸ ⚛ Ớ ᐲ ෸ ߮ ⓸ ⚛ ⽶ ⩄ ㊂ ߩ ⚻ ᤨ ᄌ ൻ ࠍ ␜ ߔ ޕ ⓸ ⚛ ⽶ ⩄ ㊂ MI0O

FC[ޔේᶧᏗ㉼୚₸ ୚ߢታ㛎ࠍ㐿ᆎߒߚ⋥ᓟ߆ࠄᓢޘߦ 0*

0 ߇ᷫ

ዋߒᆎ߼ࠆߣหᤨߦ 01

0 ߇Ⴧടߒޔᵹ౉ߩ 0*

0 ߩ߶߷ో㊂߇ 01

0 ߦ㉄ൻ ߐࠇߚޕߘߩߚ߼⽶⩄㊂ࠍ MI0O

FC[ ߦ㜞߼ߚߣߎࠈޔಣℂ᳓ߩ 0*

0㨯 01

0 Ყ߇⚂ ߦߥߞߚޕߘߩᓟޔMI0O

FC[ ߦ਄᣹ߐߖࠆ߹ߢޔㇱಽ੝

⎣㉄ൻಣℂߪ㆐ᚑߐࠇߚޕ⽶⩄㊂ MI0O

FC[ ᦼ㑆ߩ ᣣ⋡ઃㄭߢߩ 0*

0 ߩᱷ⇐ߪᢔ᳇▤ߩ⋡⹣߹ࠅߣ &1 ࡔ࡯࠲ߩ࠻࡜ࡉ࡞ߣޔᦑ᳇ਇ⿷ߢ⎣ൻ෻ᔕ߇⿠

ߎࠄߥ߆ߞߚߚ߼ߢ޽ࠆޕߎߩᦼ㑆ߩ 0*

0㨯01

0 Ყߪᐔဋߢ ߢ޽ߞߚޕ ߘߩᓟޔήᏗ㉼ߩේ᳓ࠍᵹ౉ߦ↪޿ᆎ߼ߚ⋥ᓟߪಣℂ߇቟ቯߒߥ߆ߞߚ߇ޔ 0*

0㨯01

0 Ყ߇ ߆ࠄ ߩ▸࿐ߢㇱಽ੝⎣㉄ൻ߇㆐ᚑߐࠇߚޕోᦼ㑆ߦ ߅޿ߡಣℂ᳓ਛߩ 01

0 Ớᐲߪ OI0. એਅߢ޽ࠅޔ01

0 ߆ࠄ 01

0 ߳ߩ㉄

ൻߪᛥ೙ߐࠇߡ޿ߚޕ

࿑㧟ߦㆆ㔌ࠕࡦࡕ࠾ࠕ㧔0*

㧕Ớᐲߩ⚻ᣣᄌൻࠍ␜ߔޕ#PVJQPKUGP ࠄߪޔ0*

Ớᐲ 㨪OI. ߢ੝⎣㉄㉄ൻ⚦⩶߇ㆬᛯ⊛ߦ㒖ኂࠍฃߌࠆߣႎ๔ߒߡ޿ࠆޕ ㆆ㔌 0*

Ớᐲߪ㧔㧕ᑼߢ᳞߼ߚޕㆆ㔌 0*

Ớᐲ߇Ᏹߦ OI. એ਄ߢ޽ߞߚߎߣ ߆ࠄޔ੝⎣㉄㉄ൻ⚦⩶ߪㆆ㔌 0*

ߦࠃࠆ㒖ኂࠍฃߌޔ01

0 ߆ࠄ 01

0 ߳ߩ㉄ൻ ߩᛥ೙ߦߟߥ߇ߞߚߣ⠨߃ࠄࠇࠆޕ

pH )) T 237 /(

6344 (

pH

3 e 10

10 ) L / N mg ( ammonia total

14 ) 17 L / mg ( NH

Free ×

×

× −

= ₊

1 2 3 4 5

9

6 8

7

㪇 㪉㪇㪇 㪋㪇㪇 㪍㪇㪇 㪏㪇㪇 㪈㪇㪇㪇 㪈㪉㪇㪇 㪈㪋㪇㪇 㪈㪍㪇㪇

㪇 㪌㪇 㪈㪇㪇 㪈㪌㪇 㪉㪇㪇 㪉㪌㪇

㪫㫀㫄㪼㩿㪻㪸㫐㫊㪀

㪥㫀㫋㫉㫆㪾㪼㫅㩷㪚㫆㫅㪺㪅㩷㩿㫄㪾㪄㪥㪆㪣㪀

㪇㪅㪇 㪇㪅㪌 㪈㪅㪇 㪈㪅㪌 㪉㪅㪇 㪉㪅㪌 㪊㪅㪇

㪥㪣㪩㪁 㩷㩿㫂㪾㪄㪥㪆㫄㪊 㪆㪻㪸㫐㪀

㪥㪟㪋㪄㪥㫀㫅㪽 㪥㪟㪋㪄㪥㪼㪽㪽 㪥㪦㪉㪄㪥㫀㫅㪽 㪥㪦㪉㪄㪥㪼㪽㪽 㪥㪦㪊㪄㪥㫀㫅㪽 㪥㪦㪊㪄㪥㪼㪽㪽 㪥㪣㪩

㪟㪩㪫㩿㪿㪀㩷㩷㪈㪉㪅㪇㩷㩷㩷㩷㪈㪇㪅㪋㩷㩷㩷㩷㩷㩷㩷㪎㪅㪉㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㪍㪅㪇㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㪉㪈㪅㪊㩷㩷㩷㩷㩷㩷㩷㪈㪊㪅㪎㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㪈㪉㪅㪇

࿑㧞 ฦᘒ⓸⚛Ớᐲߣ⓸⚛⽶⩄㊂ߩ⚻ᣣᄌൻ

0.4⓸⚛⽶⩄㊂

㪇 㪈㪇 㪉㪇 㪊㪇 㪋㪇 㪌㪇 㪍㪇 㪎㪇 㪏㪇 㪐㪇 㪈㪇㪇

㪇 㪌㪇 㪈㪇㪇 㪈㪌㪇 㪉㪇㪇 㪉㪌㪇

㪫㫀㫄㪼㩿㪻㪸㫐㫊㪀 㪝㫉㪼㪼㩷㪥㪟㪊㩷㪚㫆㫅㪺㪅㩿㫄㪾㪆㪣㪀

࿑ ㆆ㔌ࠕࡦࡕ࠾ࠕỚᐲߩ⚻ᤨᄌൻ

ԙ

2') ߢ൮᜝࿕ቯൻߒߚ #PCOOQZ ࡍ࡟࠶࠻ࠍᵴ↪ߔࠆㇱಽ੝⎣㉄ൻಣℂߒߚࡃ

ࠗࠝࠟࠬࡊ࡜ࡦ࠻⣕㔌ᶧߩ⓸⚛㒰෰

࿑㧠ߦ␜ߔኈⓍ . ߩࠟ࡜ࠬ⵾࡝ࠕࠢ࠲ࠍ↪޿ߡⴕߞߚޕ2') ߢ൮᜝࿕ቯߒߚ

#PCOOQZ ࡍ࡟࠶࠻ࠍ లႯߒߚޕ⚂ TRO ߢᠣᜈߒޔᜂ૕ߣᵹ౉᳓ࠍᷙวߐ ߖߚޕR* ߪ R* ࠦࡦ࠻ࡠ࡯࡜ࠍ↪޿ߡ એਅߦ⸳ቯߒޔ᳓᷷ߪ ͠ߦ଻ߞߚޕ R* ⺞ᢛᶧߦߪ *%N㧔0㧕ࠍ૶↪ߒߚޕଏ⹜ᑄ᳓ߪㇱಽ੝⎣㉄ൻಣℂ᳓ࠍ⓸⚛

⽶⩄㊂ߦวࠊߖߡ᳓㆏᳓ߢᏗ㉼ߒޔᵹ౉᳓ߣߒߚޕᵹ౉᳓ߩ &1 ߇ OI. એਅ

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