ᐕᐲߩ⋡⊛࡮⋡ᮡ㨉

᳓ⅣႺᳪᨴ‛⾰ߩࡃࠗࠝ࡮࡟ࡒ࠺ࠖࠛ࡯࡚ࠪࡦ㗔ၞߦ߅޿ߡߪޔԘᄤὼࡎ࡞ࡕࡦ᭽‛⾰ߩ ህ᳇⊛߅ࠃ߮ᅢ᳇⊛᧦ઙਅߢߩ↢‛ቇ⊛ಣℂᯏ᭴ߩᬌ⸛ޔ߅ࠃ߮ԙⅣႺ⽶⩄シᷫߩߚ߼ߦᄢ

⼺ᾚ᳝ߩ᦭ല೑↪ࠍ⋡⊛ߣߒߡ⵾ㅧߒߚ㉯ㅧ㈶ߦ฽߹ࠇࠆᯏ⢻ᕈ‛⾰ߦ㑐ߔࠆᬌ⸛ࠍⴕߞߚޕ

߹ߚޔ᳓ⅣႺᳪᨴ‛⾰ߩ↢‛ࡕ࠾࠲࡝ࡦࠣ㗔ၞߦ߅޿ߡߪޔᐔᚑ ᐕᐲߦᒁ߈⛯޿ߡޔԚᐓ ẟߩᵺൻ⢻ౣ↢ᛛⴚߩ㐿⊒ࠍ⋡ᜰߒߚޕ

㧞㧚⎇ⓥᚑᨐ

2.1 ህ᳇߅ࠃ߮ᅢ᳇᧦ઙߢߩᄤὼࡎ࡞ࡕࡦߩಽ⸃ߣ㑐ਈߔࠆᓸ↢‛⟲㓸ߩ⸃ᨆ (1) ✜⸒

ౝಽᴲᠣੂ‛⾰ߦࠃࠆ↢ᘒ♽߳ߩᓇ㗀߇໧㗴ߣߥߞߡ޿ࠆ߇ޔౝಽᴲᠣੂ‛⾰ߣߒߡߪൻቇว ᚑ‛⾰ߛߌߢߥߊᄤὼߦሽ࿷ߔࠆࡎ࡞ࡕࡦ䋨ᄤὼࡎ࡞ࡕࡦ䋩߇ήⷞߢ߈ߥ޿ߎߣ߇ࠊ߆ߞߡ߈ߚޕᄤ ὼࡎ࡞ࡕࡦߣߪࡅ࠻߿ኅ⇓ߩឃߖߟ‛ߦ฽߹ࠇࠆ߽ߩߢޔ૕ౝߢ૶↪ᷣߺߣߥߞߚࡎ࡞ࡕࡦߢ޽

ࠆޕߎߩਛߢ߽17ȕ-ࠛࠬ࠻࡜ࠫࠝ࡯࡞^䋨E2^䋩ߥߤߩᄤὼᅚᕈࡎ࡞ࡕࡦ߇᳓᫮↢‛ߦᓇ㗀ࠍਈ߃ ߡ޿ࠆߣ⸒ࠊࠇߡ޿ࠆޕ

ࡅ࠻߿ኅ⇓ߩឃᴭ‛ߪਅ᳓ಣℂ႐ߥߤߢಣℂߐࠇⅣႺਛߦ᡼ᵹߐࠇࠆޕᄤὼࡎ࡞ࡕࡦߩಽ⸃

₸ߪಣℂᣉ⸳ߩ᭴ㅧ߿․ᕈߥߤߦࠃࠅ⇣ߥࠆ߇ޔᄙߊߩಣℂᕈᣉ⸳ߢߪᅢ᳇߿ህ᳇᧦ઙߢߩ↢‛

ಣℂ߇ⴕࠊࠇߡ߅ࠅޔ↢‛ಣℂߢߩಽ⸃․ᕈࠍ⍮ࠆߎߣߪ㊀ⷐߢ޽ࠆޕߘߎߢᧄ⎇ⓥߢߪޔᄤὼ ࡎ࡞ࡕࡦߩᓸ↢‛ಽ⸃ߦ⌕⋡ߒޔᅢ᳇߅ࠃ߮ህ᳇᧦ઙߢߩᄤὼࡎ࡞ࡕࡦߩಽ⸃ߣ㑐ਈߔࠆᓸ↢‛

⟲㓸ߩ⸃ᨆࠍⴕ޿ޔᄤὼࡎ࡞ࡕࡦߩᓸ↢‛ಽ⸃ߦ㑐ߔࠆ⍮⷗ࠍᓧࠆߎߣࠍ⋡⊛ߣߒߡ⎇ⓥࠍⴕߞ ߚޕ

(2) ታ㛎᧚ᢱ߅ࠃ߮ᣇᴺ

ህ᳇ಣℂߢߪޔᮏౝ᷷ᐲࠍ37͠ߦ೙ᓮߒߚቢోᷙวဳ࡝ࠕࠢ࠲࡯ߦޔህ᳇ᕈਛ᷷ᶖൻᳪᵆ

ၭ㙃♽ࠍ᭴▽ߒߚޕᅢ᳇ಣℂߢߪޔᮏౝ᷷ᐲࠍ30͠ߦ೙ᓮߒߚᴉᲚㇱߣᦑ᳇ㇱ߆ࠄߥࠆ࡝ࠕ

ࠢ࠲࡯ߦᦑ᳇ᮏᳪᵆࠍ⒳ᳪᵆߣߒޔࠛࠬ࠻ࡠࡦ䋨E1^䋩฽᦭วᚑᑄ᳓ࠍଏ⛎ߔࠆㅪ⛯ၭ㙃♽ࠍ᭴

▽ߒߚޕE2߅ࠃ߮E1ߩ᷹ቯߦߪ᛫ේ᛫૕෻ᔕࠍ೑↪ߒߚELISAᴺޔࠛࠬ࠻ࡠࠥࡦᵴᕈߩ᷹

ቯߦߪࡅ࠻ߩࠛࠬ࠻ࡠࠥࡦ࡟࠮ࡊ࠲࡯ࠍ⚵ߺㄟࠎߛ㉂Უࠍ↪޿ࠆ㉂ᲣTwo-Hybridᴺޔਛ㑆ઍ

⻢↥‛ߩ᷹ቯߦߪGC/MSᴺࠍ૶↪ߒߚޕ߹ߚᄤὼࡎ࡞ࡕࡦߩಽ⸃ߦ㑐ਈߔࠆᓸ↢‛⟲㓸ߩ⸃

ᨆߦߪ16S rRNAㆮવሶߦၮߠߊ♽⛔⸃ᨆࠍⴕߞߚޕ

(3) ታ㛎⚿ᨐ߅ࠃ߮⠨ኤ

ህ᳇ಣℂߢߪޔଏ⛎ᶧߩTOCỚᐲ8 g/lޔᏗ㉼₸0.05 d^-1ߩ᧦ઙߢଏ⛎ߐࠇߚE2㧔100 ȝg/l㧕ߩ

90%એ਄߇ᬌ಴ߐࠇߥߊߥߞߚ߇ޔE2ଏ⛎㐿ᆎߣߣ߽ߦE2ࠃࠅ߽ᒙ޿ࠛࠬ࠻ࡠࠥࡦᵴᕈࠍ

᦭ߔࠆE1߇⚂60ȝg/l߹ߢჇടߒߚޕߎࠇࠃࠅޔህ᳇᧦ઙߢE2ߪᓸ↢‛ߦࠃࠅE1ߦ㉄ൻߐ ࠇࠆߎߣ߇␜ໂߐࠇߚޕE2߇E1ߦ㉄ൻߐࠇࠆߎߣߦࠃࠅޔ㉂ᲣTwo-Hybridᴺߦࠃࠆࠛࠬ࠻

ࡠࠥࡦᵴᕈߪ⚂60%ᷫዋߒߡ޿ߚޕGC/MSᴺߦࠃࠆਛ㑆ઍ⻢↥‛ߩ᷹ቯߢߪޔE1એᄖߦ

17ǩ-ࠛࠬ࠻࡜ࠫࠝ࡯࡞߇⚂1 µg/lᬌ಴ߐࠇߚޕଏ⛎ߐࠇߚE2ߦኻߒߡᒰ㊂ߩઍ⻢↥‛߇↢ᚑߐࠇߡ޿

ߥ޿ߩߢGC/MSߢᦝߥࠆઍ⻢↥‛ࠍ⺞ᩏߔࠆᔅⷐ߇޽ࠆޕE1ߩಽ⸃₸ࠍߐࠄߦ㜞ߊߔࠆߚ߼ߦޔ

ଏ⛎ᶧߩ㈼㉄Ớᐲࠍ਄ߍߡᬌ⸛ߒߚ߇ޔଏ⛎ᶧߩ㈼㉄ỚᐲߩჇടߦ઻޿ޔE1ߩ⫾Ⓧ㊂߽Ⴧടߒቢ

ోಽ⸃ߔࠆߎߣߪߢ߈ߥ߆ߞߚޕ

ଏ⛎ᶧߩTOCỚᐲ8 g/lޔᏗ㉼₸0.05 d^-1ߩ᧦ઙߢߩᮏౝߩ⌀ᱜ⚦⩶㧔Bacteria㧕ࠍ16S rRNAㆮ વሶߦၮߠߊ♽⛔⸃ᨆࠍⴕߥߞߚ⚿ᨐޔᦨ߽ఝභߒߡ޿ߚᓸ↢‛ߪ㈼㉄ࠍ὇⚛Ḯߣߒߚਛ᷷ࡔ

࠲ࡦ⊒㉂ߢᬌ಴ߐࠇߚProteobacteria㐷ߣFirmicutes㐷ߦㄭ✼ߥᓸ↢‛ߢ޽ߞߚޕᤃಽ⸃ᕈ‛⾰

ߢ޽ࠆ㈼㉄ࠍಽ⸃ߔࠆߣ߈ߦE2߽ಽ⸃ߔࠆ౒㉄ൻߪ⿠ߎࠄߕޔᤃಽ⸃ᕈ‛⾰ߛߌࠍㆬᛯ⊛ߦಽ⸃

ߔࠆᓸ↢‛߇ᮏౝߦߪఝභߒߡ޿ࠆߩߢߪߥ޿߆ߣ⠨߃ࠄࠇߚޕ੹࿁ⴕߞߚ16S rRNAㆮવሶߩ

ࠢࡠ࡯ࡦ⸃ᨆߢޔህ᳇᧦ઙߢE2߹ߚߪE1ࠍಽ⸃ߔࠆᓸ↢‛ߩหቯߪߢ߈ߥ߆ߞߚߩߢޔ੹ᓟߪ

Stable Isotope Probing㧔SIP㧕ᴺߥߤߩᚻᴺࠍ↪޿ߡᄤὼࡎ࡞ࡕࡦߩಽ⸃ߦ⋥ធ㑐ਈߔࠆᓸ↢‛

ࠍ․ቯߔࠆޕ

ᅢ᳇᧦ઙߢߪህ᳇᧦ઙߢಽ⸃ߢ߈ߥ߆ߞߚ E1 ߇ቢోߦಽ⸃ߢ߈ߚޕ࡝ࠕࠢ࠲࡯ᮏౝᶧ߆ࠄ 2

⒳㘃ߩᣇᴺߢE1ಽ⸃⩶ࠍන㔌ߒߚޕ੹ᓟޔߎࠇࠄߩන㔌ᩣߩหቯ߅ࠃ߮E1ಽ⸃⢻ߩ⹏ଔࠍⴕ߁ޕ

2.2 ᄢ⼺ᾚ᳝߆ࠄ⵾ㅧߒߚ㉯ㅧ㈶ߦ฽߹ࠇࠆᯏ⢻ᕈ‛⾰ߦ㑐ߔࠆᬌ⸛

ࠊࠇࠊࠇߪ㧘㤥ᄢ⼺⚊⼺⵾ㅧᎿ⒟߆ࠄ೽↢ߔࠆ㤥ᄢ⼺ᾚ᳝ࠍ᦭ലߦౣ೑↪ߔࠆߚ߼ߦ㉯ㅧ

㈶ࠍ⵾ㅧߒޔߘߩᯏ⢻ᕈߦߟ޿ߡ⺞ߴߚޕ৻⥸ߩᏒ႐ߦ߅޿ߡޔᄢ⼺ᚑಽࠍ೑↪ߒߚ㈶ߪᄙ ߊ⷗ࠄࠇࠆ߇ޔᄢ⼺ᾚ᳝ߘߩ߽ߩࠍ↪޿ߡࠕ࡞ࠦ࡯࡞⊒㉂߅ࠃ߮㈶㉄⊒㉂ࠍⴕ޿ޔ਄Ꮢߐࠇ ߡ޿ࠆ໡ຠߪߥ޿ޕ㤥ᄢ⼺ᾚ᳝㉯ㅧ㈶ߩin vivoߦ߅ߌࠆ᛫⣲≌ᵴᕈߩ૞↪ࡔࠞ࠾࠭ࡓࠍᬌ⸛

ߒߚ⚿ᨐޔ㈶㉄ࠛ࠴࡞᛽಴↹ಽ߇නᩭ⃿ᕈ⊕ⴊ∛⚦⢩㧔U937㧕ߦኻߒߡࠕࡐ࠻࡯ࠪࠬࠍ⺃ዉ ߔࠆߎߣࠍ᣿ࠄ߆ߦߒޔin vivoߦ߅ߌࠆ᛫⣲≌ᵴᕈᯏ᭴ߩ৻ߟߩน⢻ᕈࠍ␜ߒߚޕߐ߹ߑ߹

ߥේᢱࠍ↪޿ߡ⵾ㅧߐࠇߚ㉯ㅧ㈶ߩࠕࡐ࠻࡯ࠪࠬ⺃ዉᵴᕈߪᢙᄙߊႎ๔ߐࠇߡ޿ࠆ߇ޔߘࠇ ࠄߦ฽߹ࠇࠆᵴᕈᚑಽߦߟ޿ߡߪ⸒෸ߐࠇߡ޿ߥ޿ޕࠊࠇࠊࠇߪޔ㤥ᄢ⼺ᾚ᳝㉯ㅧ㈶߆ࠄޔ U937⚦⢩ߦኻߒߡ40㨪80ȝg/mlߩỚᐲߢࠕࡐ࠻࡯ࠪࠬࠍ⺃ዉߔࠆCompound-6ࠍන㔌ߔࠆߎ ߣߦᚑഞߒߚޕߎࠇ߹ߢႎ๔ߩ޽ࠆ㒢ࠅߢߪޔ㉯ㅧ㈶ਛ߆ࠄࠕࡐ࠻࡯ࠪࠬ⺃ዉ‛⾰߇න㔌࡮

♖⵾ߐࠇߚߩߪೋ߼ߡߣᕁࠊࠇࠆޕ

ࡊ࠻ࡈࠜ࡯࡞ߣ᭴ㅧ߇㘃ૃߒߡ޿ࠆI3C߅ࠃ߮ߘߩੑ㊂૕ߢ޽ࠆDIMߣ߽㘃✼㑐ଥߦ޽ࠆߎ ߣ߇␜ໂߐࠇߚߚ߼ޔߎࠇࠄߩ᭴ㅧ⸃ᨆ⚿ᨐ߿↢ℂᵴᕈߩ⋧੕㑐ଥ߆ࠄ Compound-6 ߩ᭴ㅧ ࠍផ᷹ߒߚޕ࠻࡝ࡊ࠻ࡈࠜ࡯࡞ߩᮡḰ‛⾰ߪ⚂2 㨙㧹ߢU937⚦⢩ߦኻߒߡDNAᢿ ൻࠍ⺃

ዉߒߚ߇ޔߘߩ㘃✼૕ߢ޽ࠆI3Cߪ⚂500 ȝMߢ⺃ዉߒޔߘߩੑ㊂૕ߢ޽ࠆDIMߪ⚂100 ȝ 㧹ߢ⺃ዉߒߚޕ࠻࡝ࡊ࠻ࡈࠜ࡯࡞ߣ I3㧯ߪ὇⚛㎮㧝ߟߩ⋧㆑ߒ߆ߥ޿ߦ߽㑐ࠊࠄߕޔ⺃ዉ⢻

ߪ⚂㧠୚ߩᏅ߇޽ࠅޔ߹ߚޔI3Cߩੑ㊂૕ߢ޽ࠆDIMߢߪ⚂5୚ߩࠕࡐ࠻࡯ࠪࠬ⺃ዉ⢻ࠍ߽

ߟߎߣ߇ࠊ߆ߞߚޕCompound-6ߪ40-80 ȝg/ml㧔100-200 ȝ㧹; ಽሶ㊂402ߣߒߡ㧕ߢU937ߦ DNAᢿ ൻࠍ⺃ዉߒߚߣ޿߁⚿ᨐߢ޽ࠅޔ࠻࡝ࡊ࠻ࡈࠜ࡯࡞ߩ2 mMߣߪᄢ߈ߥ⺃ዉ⢻ߩᏅ ߇ߺࠄࠇߚޕߎߩ⚿ᨐߪޔCompound-6ߣ࠻࡝ࡊ࠻ࡈࠜ࡯࡞ߩ᭴ㅧߦ⋧㆑߇޽ࠆߣ޿߁ࠊࠇࠊ ࠇߩផኤࠍᒝ߼ࠆ߽ߩߢ޽ߞߚޕ߹ߚޔCompound-6ߣ࠻࡝ࡊ࠻ࡈࠜ࡯࡞ߩ᭴ㅧߩ㆑޿߇↢ℂ ᵴᕈ୯ߩ㆑޿ߦ⃻ࠇࠆߎߣࠍ੍ᗐߒߡޔ◲ᤃ⊛ߦ⹏ଔߔࠆߎߣ߇ߢ߈ࠆ DPPH ࡜ࠫࠞ࡞ᶖ෰

ᵴᕈࠍ᷹ቯߒߚޕߘߩ⚿ᨐޔᧄታ㛎ߦ߅޿ߡ߽ Compound-6 ߣ࠻࡝ࡊ࠻ࡈࠜ࡯࡞ߩ㑆ߦᄢ߈ ߥᏅ߇⷗ࠄࠇߚޕߘߒߡޔߘߩᵴᕈߩᏅߪޔ࠻࡝ࡊ࠻ࡈࠜ࡯࡞ߣߘߩ2㊂૕ߢ޽ࠆDIMߣߩ Ꮕߣ⋧㑐߇⷗ࠄࠇޔCompound-6߇࠻࡝ࡊ࠻ࡈࠜ࡯࡞ߩੑ㊂૕ߢ޽ࠆߣ޿߁᭴ㅧ⸃ᨆ߆ࠄߩផ ኤࠍഥߌࠆ߽ߩߢ޽ߞߚޕߎߩࠃ߁ߦޔ᭴ㅧߩ㘃ૃߒߡ޿ࠆ‛⾰㑆ߩ↢ℂᵴᕈ୯ߩ⋧㆑ߪ㕖 Ᏹߦ⥝๧ᷓ޿⎇ⓥ⚿ᨐߢ޽ߞߚޕ‛⾰ߩ᭴ㅧߣߐ߹ߑ߹ߥ↢ℂᵴᕈ୯ߩ㑐ଥ߿ޔ᭴ㅧߦࠃࠆ

ၭ㙃⚦⢩ߩᔕ╵ᕈߪන⚐ߢߪߥߊޔߣߡ߽ⶄ㔀ߥ߽ߩߣ⠨߃ࠄࠇࠆޕߎࠇߪޔ⚦⢩ౝ࠲ࡦࡄ

ࠢ⾰ߩ⊒⃻߇⚦⢩⒳ߦࠃߞߡ⇣ߥࠆߎߣ߿ޔ⚦⢩߇↢ℂᵴᕈ‛⾰ߦኻߒߡ⠴ᕈࠍ߽ߟࠃ߁ߦ ߥࠆߎߣߥߤߩⷐ࿃߇޽ࠆߣ޿ࠊࠇߡ޿ࠆ߇ޔߘߩ⹦⚦ߦߟ޿ߡߪ⸃᣿ߐࠇߡ޿ߥ޿ޕ‛⾰

ߩ᭴ㅧߩ․ᓽߣ᭽ޘߥ↢ℂᵴᕈߩᒝᐲߣߩ㑐ㅪᕈ߇⸃᣿ߐࠇࠇ߫ޔᵴᕈ‛⾰ࠍੱὑ⊛ߦวᚑ ߔࠆߎߣ߇น⢻ߦߥࠆ߆߽ߒࠇߥ޿ޕ

Compound-6ߪᱜᏱ⊕ⴊ⃿⚦⢩ߦኻߔࠆ⚦⢩Ქᕈ߇U937⚦⢩ߣᲧセߒߡ᦭ᗧߦૐߊޔࠕࡐ

࠻࡯ࠪࠬࠍ⺃ዉߒߥ޿ߎߣࠍ᣿ࠄ߆ߦߒޔߘߩⷐ࿃ࠍ⸃᣿ߔࠆߚ߼ޔࠕࡐ࠻࡯ࠪࠬ⺃ዉࡔࠞ

࠾࠭ࡓߩ⸃᣿ࠍ⹜ߺߚޕCompound-6ߪࠕࡐ࠻࡯ࠪࠬ⺃ዉ૞↪ࠍߟ߆ߐߤࠆਥⷐ㉂⚛ߢ޽ࠆࠞ

ࠬࡄ࡯࠯8߅ࠃ߮3ࠍᵴᕈൻߒޔࠞࠬࡄ࡯࠯9ߪᵴᕈൻߒߥ߆ߞߚޕᰴߦޔࠞࠬࡄ࡯࠯8ߩ

਄ᵹ㗔ၞߩ૞↪ᯏ᭴ࠍ⺞ߴߚߣߎࠈޔCompound-6ߪDR5ࠍ฽ࠎߛDISCߩᒻᚑࠍଦㅴߐߖࠆ ߎߣ߇᣿ࠄ߆ߦߥࠅޔDR5 ࠍ⚻↱ߒߡࠕࡐ࠻࡯ࠪࠬ߇ᗖ⿠ߐࠇࠆߣ޿߁ Compound-6 ߩ⺃ዉ ࡔࠞ࠾࠭ࡓࠍឭ໒ߒߚޕ

Compound-6ߩ↢ᚑ↱᧪ࠍ⺞ߴߚߣߎࠈޔේᢱਛߩ࠻࡝ࡊ࠻ࡈࠔࡦࠍ㉂Უ߇ᄌ឵ߒߚੑᰴઍ

⻢↥‛ߢ޽ࠆߎߣ߇ࠊ߆ࠅޔCompound-6ߪ㤥ᄢ⼺ᾚ᳝㉯ㅧ㈶ߛߌߢߪߥߊޔ࠻࡝ࡊ࠻ࡈࠔࡦ ߇ේᢱߦ฽߹ࠇޔ㉂Უߦࠃߞߡ⊒㉂ߐࠇߚ↥‛ߢ޽ࠇ߫ේᢱߩ⒳㘃ߦ㒢ࠄߕ↢ᚑߐࠇߡߊࠆ

‛⾰ߢ޽ࠆߎߣࠍ᣿ࠄ߆ߦߒߚޕ߹ߚޔ࠻࡝ࡊ࠻ࡈࠔࡦߪࠕࡐ࠻࡯ࠪࠬࠍ⺃ዉߒߥ߆ߞߚ߇ޔ

Compound-6ߦᄌ឵ߐࠇࠆߎߣߦࠃߞߡU937⚦⢩ߦኻߒߡૐỚᐲߢࠕࡐ࠻࡯ࠪࠬࠍ⺃ዉߔࠆ

ߎߣ߇␜ߐࠇߚޕ

㤥ᄢ⼺ᾚ᳝㉯ㅧ㈶߆ࠄන㔌ߐࠇߚ Compound-6 ߪ㤥ᄢ⼺ᾚ᳝ߦ․⇣⊛ߢߪߥߊޔ☨ޔ⦱߿

㤈὾㈪☺߅ࠃ߮὾㈪☺߆ࠄ⵾ㅧߒߚ㉯ㅧ㈶ߦ߽฽߹ࠇߡ޿ࠆߎߣ߇ࠊ߆ߞߚޕ὾㈪☺ߪ὾㈪

ߩ⵾ㅧㆊ⒟ߢᄢ㊂ߦ೽↢ߔࠆ߽ߩߢ޽ࠅޔએ೨ߪᶏᵗᛩ᫈߿὾ළߦࠃߞߡಣಽߐࠇߡ޿ߚ߇ޔ

὾㈪☺߆ࠄኈᤃߦ᦭↪‛⾰ࠍ᛽಴ߔࠆᛛⴚ߇⏕┙ߐࠇࠇ߫ޔఝࠇߚ࡝ࠨࠗࠢ࡞ᣇᴺߦߥࠆߣ

⠨߃ࠆޕᄢ⼺ᾚ᳝㉯ㅧ㈶߿ฦ⒳὾㈪☺ਛߩ Compound-6฽᦭㊂ࠍ⺞ߴߚߣߎࠈޔߘࠇࠄ 1 kg

⢩ߦኻߒߡ40-80 ȝg/mlߩỚᐲߢࠕࡐ࠻࡯ࠪࠬࠍ⺃ዉߔࠆ߇ޔCompound-6߇↢૕ߦ߅޿ߡታ 㓙ߦߤߩߊࠄ޿ߩല₸ߢዊ⣺߆ࠄๆ෼ߐࠇޔࠟࡦ⚦⢩ߦኻߒߡߤߩࠃ߁ߦ૞↪ߔࠆ߆ޔ߹ߚޔ

⵾ㅧߒߚ㤥ᄢ⼺ᾚ᳝㉯ㅧ㈶ߩin vivoߢߩታ㛎⚿ᨐߦߤߩߊࠄ޿ነਈߒߡ޿ࠆߩ߆ߪޔᧄ⎇ⓥ

ߩߺߢߪోߊਇ᣿ߢ޽ࠆޕ߹ߚޔ਎⇇ߩ㘩ຠᯏ⢻ᕈ⎇ⓥߦ߅޿ߡࡅ࠻⥃ᐥ⹜㛎ޔേ‛⹜㛎߅ ࠃ߮⚦⢩ၭ㙃⹜㛎ࠍ᣿⏕ߦ㑐ㅪߠߌߡ⺑᣿ߒߚႎ๔ߪߥ޿ߚ߼ޔ‛⾰ߩᕈ⾰ߣ៨ข㊂ߦኻߔ ࠆዊ⣺ౝߢߩๆ෼ߦ߅ߌࠆ㑐ㅪᕈߩ⸃᣿߇ᦸ߹ࠇࠆޕ

2.3 ᐓẟߩᵺൻ⢻ౣ↢ᛛⴚߩ㐿⊒

(1) ✜⸒

ᐓẟⅣႺߩᖡൻ߇ޔ᦭ᯏ‛ߩᵹ౉㊂Ⴧടߣᐓẟᐩ⾰ߩ᦭ᯏ‛ಽ⸃⢻ߩૐਅ߆ࠄ↢ߓࠆ⽺㉄

⚛ൻ╬ࠍේ࿃ߣߒߚ⎫ൻ᳓⚛ߩ⊒↢ߦ⿠࿃ߔࠆߣ⠨߃ߚ႐วޔᐩ⾰ⅣႺࠍᅢ᳇ⅣႺߦߒޔᅢ

᳇ᕈᓸ↢‛ߩ௛߈ࠍ㜞߼ߡ᦭ᯏ‛㊂ࠍᷫዋߐߖࠆᡷༀᛛⴚ߇᦭ലߢ޽ࠆߣ⠨߃ࠄࠇࠆޕᐩ⾰

ਛߩᓸ↢‛ฌߪᐩ⾰ߩᅢ᳇࡮ህ᳇⁁ᘒ߿ᩕ㙃⁁ᘒߣᒝߊ⋧㑐ߒߡ߅ࠅޔⅣႺᄌേߦᢅᗵߢ޽

ࠆޕߒߚ߇ߞߡޔᐩ⾰ⅣႺߩ⁁ᘒࠍࡕ࠾࠲࡝ࡦࠣߔࠆᜰᮡߣߒߡᓸ↢‛ฌࠍ೑↪ߔࠆޕࠊࠇ ࠊࠇߪޔᐓẟᐩ⾰ߩᓸ↢‛ᯏ⢻ࠍ⸃ᨆߒޔ⸃ᨆ⚿ᨐࠍᡷༀᛛⴚߩ㐿⊒ߦ೑↪ߔࠆߎߣࠍ⺖㗴 ߣߔࠆޕᡷༀᛛⴚߩᓸ↢‛ߦ෸߷ߔᓇ㗀ߦߟ޿ߡޔ⓸⚛޽ࠆ޿ߪ⎫㤛ઍ⻢ߦ㑐ਈߔࠆᓸ↢‛

ࠍᬌ⸛ߔࠆߚ߼ߦޔᯏ⢻ᕈㆮવሶߦ⌕⋡ߒߚಽሶ↢ᘒቇ⊛ᚻᴺࠍ↪޿ߚ⸃ᨆࠍታᣉߒߚޕ (2) ታ㛎ᣇᴺ

ᐩ⾰ࠍᅢ᳇᧦ઙߦߔࠆߚ߼ߦᓥ᧪߆ࠄⴕࠊࠇߡ߈ߚᚻᴺߢ޽ࠆࠛࠕ࡟࡯࡚ࠪࡦ߿⠹⠼ᴺࠍޔ ᐩ⾰ⅣႺᡷༀࠪࡒࡘ࡟࡯࡚ࠪࡦⵝ⟎ࠍ↪޿ߡቶౝߢౣ⃻ߒޔ⇣ߥࠆ㉄ൻㆶర㔚૏ߢߩᓸ↢‛

ฌߩ⸃ᨆޔᅢ᳇ᕈᓸ↢‛ߩఝභᐲߩ⹏ଔޔ᦭ᯏ‛ߩಽ⸃⢻ߩ⹏ଔࠍⴕߞߚޕߐࠄߦޔੱᎿᎽ

ⓣߩᐩ⾰ᡷༀലᨐߦߟ޿ߡ߽ቶౝታ㛎ࠍⴕ޿ޔᐩ⾰ⅣႺᡷༀࠪࡒࡘ࡟࡯࡚ࠪࡦⵝ⟎ߢᓧࠄࠇ ߚ⚿ᨐ㧔ᓸ↢‛ฌߩᄌൻޔᐩ⾰ⅣႺޔᵺൻ⢻ജ㧕ߣᲧセߒߚޕߘߒߡޔੱᎿᎽⓣߩᵺൻ⢻ࠍ ᦨᄢߣߔࠆ᧦ઙᬌ⸛㧔Ꮍⓣᒻ⁁ޔ㈩⟎╬㧕ࠍⴕߞߚޕ߹ߚޔ᦭᣿ᶏ⃻࿾ੱᎿᎽⓣ⸳⟎ᓟߩᓸ

↢‛ฌߩࡕ࠾࠲࡝ࡦࠣࠍⴕ߁ߎߣߦࠃࠅޔ⃻࿾ᡷༀലᨐࠍ᣿ࠄ߆ߦߒߚޕ (3) ⚿ᨐߣ⠨ኤ

1)ቶౝ⠹⠼⹜㛎

ቶౝ⠹⠼ⵝ⟎ߣᾢᧄᏒ᧻የ↸ߩ⊖⽾᷼ߩᐩ⾰ࠍ↪޿ߡቶౝ⠹⠼⹜㛎ࠍⴕߞߚޕ⠹⠼ㇱߩᐩ

⾰ߩORPߪ⠹⠼1ᣣ⋡߆ࠄჇടߒޔ3ᣣ⋡ߦ70 mV߹ ߢ਄߇ߞߚ߇ޔ⠹⠼ࠍⴕࠊߥ޿ᐩ⾰ߩORPߪ3ᣣ⋡߹ߢ

߶ߣࠎߤᄌൻߒߥ߆ߞߚޕ⠹⠼ߦࠃࠅᐩ⾰ߪᅢ᳇ⅣႺߦ

ࠪࡈ࠻ߔࠆߎߣ߇ࠊ߆ߞߚޕ⎫㉄ㆶర⚦⩶ߩdsr^Bㆮવሶ ࠍPCRߦࠃࠅჇ᏷ߒߚߣߎࠈޔ⠹⠼ߒߥ޿ㇱಽߩߔߴߡ ߩࠨࡦࡊ࡞ߦ߅޿ߡჇ᏷߇ߢ߈ߚߩߦኻߒߡޔ⠹⠼ㇱߩ ࠨࡦࡊ࡞ߦ߅޿ߡߪ⠹⠼ߩ 3 ᣣ⋡߆ࠄჇ᏷߇ߢ߈ߥߊߥ ߞߚޕߒߚ߇ߞߡޔ⎫㉄ㆶర⚦⩶ߪ⠹⠼ߦࠃࠅᷫዋߒߚ ߣ⠨߃ࠄࠇߚޕߒ߆ߒޔ⠹⠼⚳ੌᓟ৻ㅳ㑆⋡ߦߪჇ᏷߇

⏕⹺ߢ߈ߚߎߣ߆ࠄޔ⠹⠼ߩലᨐߪ⍴ᦼ㑆ߒ߆ᜬ⛯ߢ߈ ߥ޿ߣ⠨߃ࠄࠇߚޕ

ᵹ㊂䋽20L/day

ᣂ㞲 ᶏ᳓

ᐩ⾰

40mm㱢 R1 600mm

400mm 1200mm

R1=300mm㱢 MAX䊄䊧䊷䊮 H2 ᶏ᳓ਛ (10-15cm)ᐩ⾰਄ጀ (25-30cm)ᐩ⾰ਛጀ

H2=300mm

ⓨ᳇

bubbling ORP᷹ቯ䉰䉟䊃

⩶ฌ⸃ᨆ䉰䉟䊃 ൻቇಽᨆ䉰䉟䊃

ᣂ㞲 ᶏ᳓

ᐩ⾰

40mm㱢 R1 600mm

400mm 1200mm

R1=300mm㱢 MAX䊄䊧䊷䊮 H2 ᶏ᳓ਛ (10-15cm)ᐩ⾰਄ጀ (25-30cm)ᐩ⾰ਛጀ

H2=300mm

ⓨ᳇

bubbling ORP᷹ቯ䉰䉟䊃

⩶ฌ⸃ᨆ䉰䉟䊃 ൻቇಽᨆ䉰䉟䊃

60mm90mm 150mm

࿑1 ቶౝੱᎿᎽⓣ⸳⟎⹜㛎ߦ

ᾢᧄᏒ᧻የ↸ߩ⊖⽾᷼߆ࠄណขߒߚᐩᵆࠍ↪޿ߡੱᎿᎽⓣⵝ⟎ࠍ┙ߜ਄ߍߚ㧔࿑1㧕ޕⓨ᳇

ߢㅢ᳇ߒߚᣂ㞲ߥੱᎿᶏ᳓ࠍੱᎿᎽⓣߩਛߦㅪ⛯ߢଏ⛎ߒߚޕੱᎿᎽⓣߩਛᔃ߆ࠄߩ〒㔌߇ 6 cmޔ9 cmޔ15 cm㔌ࠇߚ࿾ὐߩᷓߐ10 cmޔ20 cmޔ30 cmߦ߅ߌࠆORPߩᄌൻࠍ࿑2 ߦ␜ߒߚޕੱᎿᎽⓣߩਛᔃ߆ࠄ6 cm㔌ࠇߚ࿾ὐߢߪޔ1ᣣ⋡߆ࠄORP߇਄᣹ߒޔታ㛎⚳ੌ

ߩ89ᣣ⋡߹ߢᓢޘߦ਄᣹ߒ⛯ߌߚޕ߹ߚޔᷓߐ10 cmߢߪᎽⓣߦㄭ޿႐ᚲ߶ߤORP߇㜞ߊޔ

ੱᎿᎽⓣߦࠃߞߡᐩ⾰ⅣႺ߇ࠃࠅᅢ᳇⊛ߥⅣႺߦࠪࡈ࠻ߒߚߣ⠨߃ࠄࠇߚޕ ੱᎿᎽⓣ⃻࿾⹜㛎ߦ߅޿ߡߪޔ

DGGE ߦࠃࠆᯏ⢻ᕈㆮવሶߩ⸃

ᨆߢᦨ߽㗼⪺ߦࡃࡦ࠼ࡄ࠲࡯ࡦߦ

⋧㆑߇⷗ࠄࠇߚߩߪޔ⎫㉄ㆶర⩶

ߩ dsrB ㆮવሶ㗔ၞࠍᮡ⊛ߣߒߚ

߽ߩߢ޽ߞߚޕ⸳⟎8ࡨ᦬ᓟએ㒠 ߢߪਛጀ߅ࠃ߮ਅጀߦ߅޿ߡ࠼࡟

࡯ࡦ࠲ࠗࡊߣ MAX ࠼࡟࡯ࡦ࠲ࠗ

ࡊߩ⸳⟎඙ၞߩࡃࡦ࠼ࡄ࠲࡯ࡦ߇ Ყセ඙ߦᲧߴߡ᣿ࠄ߆ߦ⭯ߊߥߞ ߡ߅ࠅޔ⎫㉄ㆶర⩶ߩࡐࡇࡘ࡟࡯

࡚ࠪࡦ߿࠳ࠗࡃ࡯ࠪ࠹ࠖߩᷫዋ߇

␜ໂߐࠇߚޕ⸳⟎8ࡨ᦬ᓟᐓẟၞ

ߩᲧセ඙߅ࠃ߮ MAX࠼࡟

࡯ࡦ࠲ࠗࡊ⸳⟎඙ࠨࡦࡊ࡞

ࠍ↪޿ߡࠢࡠ࡯ࡦ⸃ᨆࠍⴕ ߞߚߣߎࠈޔ⎫㉄ㆶర⩶ࠍ

฽߻ህ᳇ᕈ⚦⩶ߦㄭ✼ߥࠢ

ࡠ࡯ࡦߩഀวߪᲧセ඙ߦᲧ ߴߡ㗼⪺ߦᷫዋߒߡ޿ߚߎ ߣ߇ࠊ߆ߞߚ㧔࿑3㧕ޕ߹ߚޔ

⸳⟎8ࡨ᦬ᓟ߆ࠄߪਛጀߣ ਅጀߛߌߢߥߊ਄ጀߦ߽ᄌ ൻ߇⃻ࠇᆎ߼ߚޕߎࠇߪޔ

૞⵾ߐࠇߚੱᎿᎽⓣ߇ᧄ᧪

ߪਛጀ߿ਅጀߣ޿ߞߚᐩ⾰

ᷓጀㇱߦኻߒߡലᨐࠍ⊒ើ

ߔࠆ߽ߩߢ޽ߞߚ߇ޔ8 ࡨ᦬߿ 9 ࡨ᦬ߣ޿߁㐳ᦼ㑆ߦࠊߚࠆ⸳⟎ࠍⴕߞߚߎߣߢޔᷓጀㇱߢ

⿠ߎߞߚᅢ᳇⊛ⅣႺ߳ߩ⒖ⴕߣ޿߁ᡷༀലᨐ߇਄ጀ߽߳વ᠞ߒߚ⚿ᨐߢ޽ࠆߣ⠨߃ࠄࠇߚޕ

ੱᎿᎽⓣ⸳⟎ߩലᨐߪ⍴ᦼ⊛ߥ߽ߩߢߪߥߊޔ㐳ᦼ⸳⟎ࠍⴕ߁ߎߣߢਛጀ߿ਅጀ߆ࠄ਄ጀ߳

ߣᡷༀലᨐ߇વ᠞ߒߡ޿ߊ߽ߩߢ޽ࠆߎߣ߇␜ໂߐࠇߚޕ 3)ᓸ↢‛ߩቯ㊂ൻ

ᡷༀലᨐࠍᓸ↢‛࡟ࡌ࡞ߢቯ㊂⊛ߦ⹏ଔߔࠆߚ߼ߦޔᐩ⾰ਛߩో⚦⩶ߣ⎫㉄ㆶర⚦⩶ߩቯ

0 5 10 15 20 25 30 35 40

Alpha Proteo Gamma Delta Proteo Epsilon Proteo Acidobacteria ActinobacteriaBacteroidetes Chloroflexi Deferribacteres Planctomycete CyanobacteriaFirmicutes OP11OP8 unclassified

Classification

Relative ratio (%) 䂓 Ყセ඙

䂓 MAX䊄䊧䊷䊮⸳⟎඙

࿑3 ⃻࿾ੱᎿᎽⓣ⸳⟎⹜㛎ߦ߅ߌࠆࠢࡠ࡯ࡦ⸃ᨆߩ⚿ᨐ 㪄㪈㪌㪇

㪄㪈㪇㪇 㪄㪌㪇 㪇 㪌㪇 㪈㪇㪇 㪈㪌㪇

㪇 㪉㪇 㪋㪇 㪍㪇 㪏㪇 㪈㪇㪇

ㆇォᦼ㑆䋨ᣣ䋩

㉄ൻㆶర㔚૏㩿㪦㪩㪧㩷㫄㪭㪀

㪍㪺㫄 㪐㪺㫄 㪈㪌㪺㫄

㪍㪺㫄 㪐㪺㫄 㪈㪌㪺㫄

㪍㪺㫄 㪐㪺㫄 㪈㪌㪺㫄

ORP:᳓⚛ၮḰ䈮឵▚䈚䈢䇯

ᷓ䈘10cm

ᷓ䈘20cm

ᷓ䈘30cm

࿑2 ቶౝੱᎿᎽⓣ⸳⟎⹜㛎ߦ߅ߌࠆᐩ⾰ߩORPᄌൻ

㊂ᣇᴺࠍᬌ⸛ߒߚޕߘߩ⚿ᨐޔ16S rRNAㆮવሶߣdsr^{ㆮવሶߦၮߠ޿ߚቯ㊂}PCRߦࠃࠆቯ

㊂ᣇᴺࠍ߶߷⏕┙ߢ߈ߚޕ

㧟㧚ᚑᨐߩ㆐ᚑᐲ

㗔ၞ㧔A㧕߅ࠃ߮㧔B㧕ߦ߅޿ߡචಽߥࠆᚑᨐࠍᓧࠆߎߣ߇ߢ߈ߚޕ

㧠㧚੹ᓟߩ⋡ᮡ

㗔ၞ㧔A㧕ߢߪޔ⃻࿾ᐓẟߦ߅ߌࠆ㉄ൻㆶర⁁ᘒߣ⎫㤛ࠨࠗࠢ࡞ߩ㑐ଥࠍ᣿ࠄ߆ߦߒޔᓸ↢

‛↢ᘒ♽ߣᐓẟⅣႺᡷༀߩ㑐ଥࠍ᣿ࠄ߆ߦߒߚ޿ޕ

㗔ၞ㧔B㧕ߢߪޔⅣႺ⽶⩄ߣߥࠆ᭽ޘߥ᦭ᯏ♽ᑄ᫈‛ߩࡔ࠲ࡦ⊒㉂߿ࠛ࠲ࡁ࡯࡞⊒㉂ࠍ೑↪

ߒߚ᦭ല೑↪ᛛⴚߦ㑐ߒߡ⛮⛯ߒߡᬌ⸛ࠍⴕ߁ޕ߹ߚޔ㘩ຠ߿㉯ㅧᎿ⒟߆ࠄឃ಴ߐࠇࠆ᦭ᯏ

♽ࡃࠗࠝࡑࠬ߆ࠄᯏ⢻ᕈ㘩ຠࠍ⵾ㅧߒޔ↢ℂᵴᕈ‛⾰ߩหቯޔ↢ℂᵴᕈ⹏ଔޔ↢ℂᵴᕈ‛⾰

ߩ↢ᚑᯏ᭴߅ࠃ߮૞↪ᯏ᭴ߩ⸃᣿ࠍⴕ߁ߎߣߢޔ⑼ቇ⊛ᩮ᜚ߦၮߠߊ㜞ઃടଔ୯ൻࠍ⋡ᜰߔޕ ߎࠇࠄߩᛛⴚߦࠃࠅޔᓥ᧪ߩਛ㑆ಣಽψၒ┙ಣಽߣ޿ߞߚᣢሽߩಣℂᛛⴚࠍ೑ᵴ↪ᛛⴚߦᄌ

឵ߔࠆߎߣߢޔ㒽਄⽶⩄ߩシᷫ߿࿾⃿᷷ᥦൻ㒐ᱛߦ⽸₂ߒߚ޿ޕ

㧡㧚ᐔᚑ19ᐕᐲߩ⎇ⓥ⾌ขᓧ⁁ᴫ

㧔㧕ฃ⸤⎇ⓥ㧔0'&1㧕ޔᐕޔ౞ޔࡃࠗࠝࡑࠬࠛࡀ࡞ࠡ࡯㜞ല₸ォ឵ᛛⴚ㐿⊒

㧛ࡃࠗࠝࡑࠬࠛࡀ࡞ࠡ࡯వዉ⎇ⓥ㐿⊒㧛ㇺᏒဳࡃࠗࠝࡑࠬ⾗Ḯ߆ࠄߩ㜞ല₸ੑᲑ⊒㉂ߦࠃࠆ Άᢱ↪ࠛ࠲ࡁ࡯࡞⵾ㅧᛛⴚߩ㐿⊒

㧔㧕ฃ⸤⎇ⓥ㧔ፏၔᄢቇ㧕ޔᐕޔ౞ޔᐔᚑᐕᐲޟᣂࠛࡀ࡞ࠡ࡯ᛛⴚ㐿⊒ࡊ ࡠࠣ࡜ࡓ㧛ᣂࠛࡀ࡞ࠡ࡯ᛛⴚ⎇ⓥ㐿⊒㧛ᣂࠛࡀ࡞ࠡ࡯ࡌࡦ࠴ࡖ࡯ᛛⴚ㕟ᣂ੐ᬺ㧔ࡃࠗࠝࡑࠬ㧕 㧛਻Ꮊ⊒ࡆ࡟࠶ࠫ࠹ࠢࡁࡠࠫ࡯᭴▽ߦะߌߚ┻߆ࠄߩࡃࠗࠝࠛ࠲ࡁ࡯࡞ᄌ឵ߩᛛⴚ㐿⊒

㧔㧕ฃ⸤⎇ⓥ㧔䋨ᩣ㧕ࠅࠀ߁ߖ߈㧕ޔᐕޔ౞ޔⅣႺ⋭ ᐔᚑᐕᐲ࿾⃿᷷ᥦൻ ኻ╷ᛛⴚ㐿⊒੐ᬺ㧔ᴒ✽↥♧Ᵽ߆ࠄߩΆᢱ↪ࠛ࠲ࡁ࡯࡞↢↥ࡊࡠ࠮ࠬ㐿⊒෸߮'╬ታ⸽ታ㛎 㧛ᴒ✽↥♧Ᵽߩㆡ↪ᕈ╬ߦ㑐ߔࠆ⎇ⓥ㐿⊒㧕

㧔㧕⑼ቇᛛⴚᝄ⥝⺞ᢛ⾌㧔ᾢᧄᄢቇઍ⴫㧦ṚᎹవ↢㧕ޔᐕޔ౞ޔᐩ⾰ᯏ⢻ߩ ᓸ↢‛ቇ⊛⹏ଔߣᡷༀᛛⴚߩᬌ⸛

㧔㧕⑼⎇⾌ޔᐕޔ౞ޔᄤὼࡎ࡞ࡕࡦ㒰෰ࠍ฽߻ኅ⇓♮ዩ࡮↢ߏߺᷙว‛ߩ㜞 ല₸ࠨ࡯ࡑ࡞࡝ࠨࠗࠢ࡞ᛛⴚߩ㐿⊒

㧔㧕౒ห⎇ⓥ㧔࿖┙ᄢቇᴺੱ⑔ፉᄢቇ㧕ޔᐕޔ౞ޔ᳓↢ᬀ‛ᱷᷲࡃࠗࠝࡑࠬ

߆ࠄߩࠕ࡞ࠦ࡯࡞⊒㉂ߦ㑐ߔࠆᛛⴚ㐿⊒⎇ⓥ

㧔㧕౒ห⎇ⓥ㧔᧲੩ⅽᣁ㧔ᩣ䋩㧕ޔᐕޔ౞ޔ↢ߏߺࠍਥේᢱߣߒߚࠕ࡞ࠦ࡯࡞

↢↥ࡊࡠ࠮ࠬ㐿⊒߅ࠃ߮ޔᒰ⹥ࠪࠬ࠹ࡓߦ᦭ലߥࠕ࡞ࠦ࡯࡞⊒㉂㉂Უߩ⢒⒳ߦ㑐ߔࠆ౒ห⎇ⓥ

㧔㧕౒ห⎇ⓥ㧔䋨ᩣ㧕ࡒ࠷ࠞࡦࠣ࡞࡯ࡊᧄ␠㧕ޔᐕޔ౞ޔਛ࿖࿕૕㈶㉄⊒㉂ߦ ߅ߌࠆ․ᓽ⊛㚅᳇↢ᚑߩᓸ↢‛ቇ⊛ࡔࠞ࠾࠭ࡓ⸃ᨆ

㧣㧚ᐔᚑ19ᐕᐲߩ⎇ⓥ⊒⴫ޔ․⸵⁁ᴫ (1) ේ⪺⺰ᢥߦࠃࠆ⊒⴫ ⸘12ઙ

1) Ⓑ၂⑲৻㇢ޔ᫪᧛ ⨃ޔᮭ⮮๺ୃޔḡ ጪℙޔ⧂Ꮉᶈᔒޔᧁ↰ᑪᰴ: ࠛ࠲ࡁ࡯࡞⊒㉂ㆊ

⒟ߢ↢ᚑߔࠆࠕࡐ࠻࡯ࠪࠬ⺃ዉ‛⾰࡮࠻࡝ࡊ࠻ࡈࠜ࡯࡞. ᣣᧄ㉯ㅧදળ⹹ޔ102, 222-224 (2007).

2) ḡ ጪℙޔችᎹඳ჻ޔየፒ✍ሶޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴ: rRNA ㆮવሶߩࠬࡍ࡯ࠨ࡯㗔ၞ

ߩႮၮ㈩೉ߦၮߠߊ὾㈪㉂Უߩ⼂೎. ᣣᧄ㉯ㅧදળ⹹ޔ102, 679-684 (2007).

b) ⧷ᢥ⹹

1) Ermawati, R., Morimura, S., Tang, Y.Q., Liu, K., Kida, K.: Degradation and behavior of natural steroid hormones in cow manure waste during biological treatments and ozone oxidation. J. Biosci.

Bioeneg.,103, 27-31 (2007).

2) Inagaki, S., Morimura, S., Gondo, K., Tang, Y.Q., Akutagawam H., Kida, K.: Isolation of tryptophol as an apoptosis-inducing component of vinegar produced from boiled extract of black soybean in human monoblastic leukemia U937 cells. Biosci. Biotechnol. Biochem., 71, 371-379 (2007).

3) Tang, Y.Q., Shigematsu, T., Morimura, S., Kida, K.: Effect of dilution rate on the microbial structure of a mesophilic butyrate-degrading methanogenic community during continuous cultivation. Appl. Microbiol. Biotechnol., 75, 451-465 (2007).

4) Inagaki, S., Morimura, S., Tang, Y.Q., Akutagawam H., Kida, K.: Tryptophol induces death receptor (DR) 5-mediated apoptosis in U937 cells. Biosci. Biotechnol. Biochem., 71, 2065-2068 (2007).

5) Takamine K, Abe J., Shimono K., Sameshima Y., Morimura S., Kida K.: Physicochemical and gelling characterizations of pectin extracted from sweetpotato pulp. J. Appl. Glycosci., l54, 211-216 (2007).

6) An, M.Z., Tang, Y.Q., Morimura, S., Kida, K.: Treatment of distillation wastewater from production process of fuel ethanol from wood biomass. Jpn. Soc. Water Treat. Biol., 43, 209-217 (2007).

7) Tang, Y.Q., Fujimura, Y., Shigematsu, T., Morimura, S., Kida, K.: Anaerobic treatment performance and microbial population of thermophilic upflow anaerobic filter reactor treating awamori distillery wastewater. J. Biosci. Bioeng.. 104, 281-287 (2007).

8) Cao, X., Tsukamoto, T., Seki, T., Tanaka, H., Morimura, S., Cao, L., Mizoshita, T., Ban, H., Toyoda, T., Maeda, H., Tatematsu, M.: 4-Vinyl-2,6-dimethoxyphenol (canolol) suppresses oxidative stress and gastric carcinogenesis in Helicobacter pylori-infected carcinogen-treated Mongolian gerbils. Int. J. Cancer. 2007 Dec 4.

9) Bin Guo, Jun Gu, Yu-Gaung Ye, Yue-Qin Tang, Kenji Kida and Xiao-Lei Wu: Marinobacter segnicrescens sp. nov., a moderate halophile isolated from benthic sediment of the South China Sea, International Journal of Systematic and Evolutionary Microbaiology, 57, 1970-1974 (2007).

10) F. Liu, S. Ye, Y. Tang, K. Takikawa, K. Kida, X. Wu: Microbial community structure analysis of the coastal wetland soil in the Yellow River Delta, Archive of Applied and Environmental Biology. 2007, No.5 (In Chinese)

1)ਛየᵏᓆޔḡ ጪℙޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޟੇᑼࡔ࠲ࡦ⊒㉂ߦ㑐ਈߔࠆᓸ↢‛⟲㓸ߩ⸃

ᨆߣ࠮࡞ࡠ࡯ࠬࠍಽ⸃ߔࠆࠦࡦ࠰࡯ࠪࠕࡓߩ᭴▽ޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢળ

⻠Ṷⷐᣦ㓸p.16, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

2)⑔↰㓶ౖޔḡ ጪℙޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޟਛ࿖㈶㉄࿕૕⊒㉂ߦ߅ߌࠆᓸ↢‛⟲㓸ߩ⸃

ᨆޠ╙ 14 ࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸 p.16, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ

(2007ᐕ12᦬1ᣣ).

3)ᐣ ᥓ⵨ޔᮮየᩕḩޔḡ ጪℙޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޟࠣ࡞ࠦ࡯ࠬࠍන৻὇⚛Ḯߣߒߚ ࡔ࠲ࡦ⊒㉂ߦ㑐ਈߔࠆᓸ↢‛⟲㓸ߩ⸃ᨆޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ 㓸p.17, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

4)ችᎹඳ჻ޔᓢ භ⪇ޔḡ ጪℙޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޟႮၮ㈩೉ߦࠃࠆ㐳ᦼߐߒ߽ߣߦ ߅ߌࠆ㉂Უߩ቟ቯᕈߩ⹏ଔޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸p.17, 㐳ፒ ᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

5)਄↰⪇ᄹሶޔ᫪ ⛗⟤ሶޔḡ ጪℙޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޟࠛ࠲ࡁ࡯࡞⊒㉂ㆊ⒟ߦ߅ߌ ࠆࠦ࡯ࡅ࡯㉄ࠛ࠴࡞ࠛࠬ࠹࡞ߩ↢ᚑᯏ᭴ߦ㑐ߔࠆᬌ⸛ޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱ ᄢળ⻠Ṷⷐᣦ㓸p.18, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

6) ㈬੗ᶈᣩޔ㐳ᵿ৻ᒄޔ᫪᧛ ⨃ޔ↰ญਭ⾆ޔ⿒᧻ 㓉ޔᧁ↰ᑪᰴޔዊᎹ㓉ᐔޔ᧻ጟᱜ૫ ޟΆᢱ↪ࠛ࠲ࡁ࡯࡞⵾ㅧߩߚ߼ߩ੃㉄࡮ࠣ࡞ࠦ࡯ࠬหᤨ⾗ൻ⊒㉂ᕈ㉂Უߩ⢒⒳ޠ╙14࿁ ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸p.18, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1 ᣣ).

7) ↱Ꮣථ਽ޔဈ↰ᐽᱜޔഏ ಫޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޔ㐳ᵿ৻ᒄޔ᧻ጟᱜ૫ޔዊᎹ㓉ᐔޟ┻

߆ࠄߩࡃࠗࠝࠛ࠲ࡁ࡯࡞↢↥ߦะߌߚ೨ಣℂᣇᴺߩᬌ⸛ޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰ ㇱᄢળ⻠Ṷⷐᣦ㓸p.19, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

8)቟ ᣿ືޔੑችౖ༹ޔḡ ጪℙޔ᫪᧛ ⨃ޔ ᧁ↰ᑪᰴޔ⿒᧻ 㓉ޔ↰ญਭ⾆ޔ᧻ጟᱜ૫ޔ ዊᳰᵗẢޟ↢ߏߺหᤨ♧ൻ࡮⊒㉂߆ࠄឃ಴ߐࠇࠆ⫳⇐ᑄᶧߩੇᑼ߅ࠃ߮Ḩᑼࡔ࠲ࡦ⊒㉂

ߩᬌ⸛ޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸p.21, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ

(2007ᐕ12᦬1ᣣ).

9)ㄞ 㤗㉿ޔ቟ ᣿ືޔḡ ጪℙޔ᫪᧛ ⨃ޔ ᧁ↰ᑪᰴޔ⿒᧻ 㓉ޔ↰ญਭ⾆ޔ᧻ጟᱜ૫ޔ ዊᳰᵗẢޟᏒ⽼㉂⚛೷ߩᵴᕈ⹏ଔߣࡃࠗࠝࠛ࠲ࡁ࡯࡞ߩ⵾ㅧޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻

Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸p.21, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

10)㊄ਛ ᷕޔഏ ಫޔḡ ጪℙޔ᫪᧛ ⨃ޔ ᧁ↰ᑪᰴޔ྾ች♿ਯޔᄢᐸ ẖޟ↠⩿ࡠ࡯

ࠫࡘ࡯ࠬ߆ࠄߩㅪ⛯⊒㉂ߦࠃࠆࡃࠗࠝࠛ࠲ࡁ࡯࡞ߩ⵾ㅧޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰ ㇱᄢળ⻠Ṷⷐᣦ㓸p.22, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

11) ች੗▸⵨ޔ↰ญਭ⾆ޔ⿒᧻ 㓉ޔḡ ጪℙޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޟ੃㉄⾗ൻᕈߢ⠴㉄

ᕈ࡮⠴ᾲᕈታ↪㉂Უߩ⢒⒳ޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸p.26, 㐳ፒ ᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

12)ች੗▸⵨ޔ↰ญਭ⾆ޔ⿒᧻ 㓉ޔḡ ጪℙޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޟಝ㓸ᕈ࡮⠴㉄ᕈࡋ࠹

ࡠ࠲࡝࠭ࡓ৻୚૕ታ↪㉂ᲣKFG4-6B߆ࠄ㕖ಝ㓸ᕈ࡮ห⾰ㆮવሶ♽⛔ᩣߩಽ㔌ޠ╙14࿁ ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸p.26, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1 ᣣ).

ᓸ↢‛ߩ 16S rRNAㆮવሶࠍᮡ⊛ߣߒߚੱᎿᎽⓣ⸳⟎ലᨐߩ⹏ଔޠ╙14 ࿁ᣣᧄ↢‛Ꮏ ቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸p.31, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

14)የፒ✍ሶޔችᎹඳ჻ޔḡ ጪℙޔ᫪᧛ ⨃ޔ ᧁ↰ᑪᰴޟr㧾㧺㧭ㆮવሶߩࠬࡍ࡯ࠨ࡯㗔

ၞߩႮၮ㈩೉ߦၮߠߊ὾㈪㉂Უߩ⼂೎ޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸 p.31, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

15)↰ਛ⑲ᄥ㇢ޔਛᎹ ⢒ޔḡ ጪℙޔ᫪᧛ ⨃ޔ ᧁ↰ᑪᰴޟ⚦⢩Ⲣวߦࠃࠆ⠴㉄ᕈ࡮⠴

ᾲᕈ㉂Უߩ⢒⒳ޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸p.32, 㐳ፒᄢቇᢥᢎࠠ

ࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

16)⼱ ஜผޔⒷ၂⑲৻㇢ޔḡ ጪℙޔ᫪᧛ ⨃ޔ ᧁ↰ᑪᰴޟU937 ⚦⢩ߦኻߔࠆࠦ࡯ࡅ

࡯㉄ࠛ࠴࡞ࠛࠬ࠹࡞ߩࠕࡐ࠻࡯ࠪࠬ⺃ዉᯏ᭴ߩ⸃᣿ޠ╙14࿁ᣣᧄ↢‛Ꮏቇળ਻Ꮊᡰㇱᄢ ળ⻠Ṷⷐᣦ㓸p.41, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

17)↰ፉ࿻␭ޔ૒⮮੫ᄥޔᲧཅర♿ޔ㑐 ቁᒄޔḡ ጪℙޔ᫪᧛ ⨃ޔ ᧁ↰ᑪᰴޟHepG2

⚦⢩ߦኻߔࠆࠦ࡯ࡅ࡯㉄ࠛ࠴࡞ࠛࠬ࠹࡞ߩ᛫࡜ࠫࠞ࡞ᵴᕈߦ㑐ߔࠆᬌ⸛ޠ╙14࿁ᣣᧄ↢

‛Ꮏቇળ਻Ꮊᡰㇱᄢળ⻠Ṷⷐᣦ㓸p.41, 㐳ፒᄢቇᢥᢎࠠࡖࡦࡄࠬ (2007ᐕ12᦬1ᣣ).

18)Takahiro Seki, Shigeru Morimura, Jun Fang, Yue-Qin Tang, Hiroshi Maeda and Kenji Kida.:̌Immunostimulation and antitumor activity of shochu distilled residues̍. International Conference on Food Factors for Health Promotion (ICOFF2007), ੩ㇺ࿖㓙ળ⼏ળ㙚, ᐔᚑ19 ᐕ11᦬27ᣣ

19)Takahiro Seki, Shigeru Morimura, Xueyuan Cao, Tetsuya Tsukamoto, Masae Tatematsu, Kenji Kida and Hiroshi Maeda.:̍Suppression of inflammation and carcinogenesis by a phenolic compound, canolol, obtained from crude rape seed oil̍. 3rd Internatinal Conference on Polyphenols and Health (ICPH2007), ੩ㇺ࿖㓙ળ⼏ળ㙚, ᐔᚑ19ᐕ11᦬25ᣣ.

20)᫪᧛ ⨃ޔ㑐 ቁᒄޔⒷ၂⑲৻㇢ޔḡ ጪℙޔᧁ↰ᑪᰴޟ㘩ຠ↥ᬺ೽↢‛೑ᵴ↪ߩߚ߼

ߩᯏ⢻ᕈ㘩ຠߩᄌ឵ޠ╙ 59 ࿁ᣣᧄ↢‛Ꮏቇળᄢળ⻠Ṷⷐᣦ㓸p. 24, ᐢፉᄢቇ᧲ᐢፉࠠ

ࡖࡦࡄࠬ (2007ᐕ9᦬27ᣣ).

21) ቟ ᣿ືޔḡ ጪℙޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޟᶏᄖ↥♧Ᵽ߆ࠄߩಝ㓸ᕈ㉂Უࠍ↪޿ߚㅪ

⛯⊒㉂ߦࠃࠆΆᢱ↪ࠛ࠲ࡁ࡯࡞ߩ↢↥ޠ╙59࿁ᣣᧄ↢‛Ꮏቇળᄢળ⻠Ṷⷐᣦ㓸p. 98, ᐢ ፉᄢቇ᧲ᐢፉࠠࡖࡦࡄࠬ (2007ᐕ9᦬25ᣣ).

22)㈬੗ᶈᣩޔ⿒᧻ 㓉ޔ↰ญਭ⾆ޔᧁ↰ᑪᰴޔዊᎹ㓉ᐔޔ㐳ᵿ৻ᒄޔ᧻ጟᱜ૫ޟࡃࠗࠝࡑ

ࠬ㜞ല₸⊒㉂ߦࠃࠆࠛ࠲ࡁ࡯࡞↢↥ߩߚ߼ߩ੃㉄⾗ൻ⊒㉂ᕈ㉂Უߩ⢒⒳ޠ╙59࿁ᣣᧄ↢

‛Ꮏቇળᄢળ⻠Ṷⷐᣦ㓸p. 123, ᐢፉᄢቇ᧲ᐢፉࠠࡖࡦࡄࠬ (2007ᐕ9᦬26ᣣ).

23) ⷏ ፏผޔᧁ↰ᑪᰴޔ᫪᧛ ⨃ޔḡ ጪℙޔ᧻੗ ᔀޟ⿥㜞᷷ࡔ࠲ࡦ⊒㉂ࡊࡠ࠮ࠬߩ

᭴▽ߣᓸ↢‛⟲㓸ߩ⸃ᨆޠ╙59࿁ᣣᧄ↢‛Ꮏቇળᄢળ⻠Ṷⷐᣦ㓸p. 176, ᐢፉᄢቇ᧲ᐢ ፉࠠࡖࡦࡄࠬ (2007ᐕ9᦬26ᣣ).

24)ᑝᳰఝ᣿ޔᧁ↰ᑪᰴޔ᫪᧛ ⨃ޔḡ ጪℙޟᄤὼࡎ࡞ࡕࡦߩህ᳇ಽ⸃߅ࠃ߮㑐ਈߔࠆᓸ

↢‛⟲㓸ߩ⸃ᨆޠ╙59࿁ᣣᧄ↢‛Ꮏቇળᄢળ⻠Ṷⷐᣦ㓸p. 176, ᐢፉᄢቇ᧲ᐢፉࠠࡖࡦ ࡄࠬ (2007ᐕ9᦬26ᣣ).

25)ḡ ጪℙޔ቟ ᣿ືޔ᫪᧛ ⨃ޔ᧻ጟᱜ૫ޔ⿒᧻ 㓉ޔᧁ↰ᑪᰴޟหᤨ♧ൻ࡮⊒㉂ߦࠃ ࠆ↢ߏߺ߆ࠄߩࡃࠗࠝࠛ࠲ࡁ࡯࡞ߩ↢↥ޠ╙59࿁ᣣᧄ↢‛Ꮏቇળᄢળ⻠Ṷⷐᣦ㓸p. 198,

26)↰ญਭ⾆ޔች੗▸⵨ޔḡ ጪℙޔ᫪᧛ ⨃ޔ ᧁ↰ᑪᰴޔ⿒᧻ 㓉ޟㆮવ੤㔀ߢ߈ࠆࠛ

࠲ࡁ࡯࡞↢↥ታ↪㉂Უߩ⢒⒳ޠ╙59࿁ᣣᧄ↢‛Ꮏቇળᄢળ⻠Ṷⷐᣦ㓸p. 213, ᐢፉᄢቇ

᧲ᐢፉࠠࡖࡦࡄࠬ (2007ᐕ9᦬26ᣣ).

27)ች੗▸⵨ޔ↰ญਭ⾆ޔḡ ጪℙޔ᫪᧛ ⨃ޔ ᧁ↰ᑪᰴޔ⿒᧻ 㓉ޟㆮવ੤㔀ߢ߈ࠆࠛ

࠲ࡁ࡯࡞↢↥ታ↪㉂Უߩ⠴㉄ᕈߣ⠴ᾲᕈޠ╙59࿁ᣣᧄ↢‛Ꮏቇળᄢળ⻠Ṷⷐᣦ㓸p. 214, ᐢፉᄢቇ᧲ᐢፉࠠࡖࡦࡄࠬ (2007ᐕ9᦬26ᣣ).

28)ᧁ↰ᑪᰴޔ᫪᧛ ⨃ޔḡ ጪℙޟ⊕㈬ߣ὾㈪ߩ⵾ㅧᴺ෸߮ᚑಽߩᲧセޠ╙4࿁㣮ఽፉᄢ ቇ὾㈪ቇࠪࡦࡐࠫ࠙ࡓ㧔╙4࿁਻Ꮊ࡮྾Ꮉ㧔ਛ࿖㧕㘩ຠ࡮㉯ㅧࠪࡦࡐࠫ࠙ࡓޔ╙33࿁ᣣ ᧄᔕ↪♧⾰⑼ቇળ਻Ꮊᡰㇱᄢળวหᄢળ㧕⻠Ṷⷐᣦ㓸, pp.16-26, 㣮ఽፉᄢቇⒷ⋓ળ㙚 (2007ᐕ9᦬10ᣣ).

29) ự↰ᐽᄥ㇢ޔ’ౝ⾆ሶޔ᧻᳗ᱜ⑲ޔᧃ᳗⾆ผޔ᳗↰ ⡡ޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޟ㤈὾

㈪☺߆ࠄߩ὾㈪߅ࠃ߮㉯ㅧ㈶ߩ⵾ㅧߩߚ߼ߩታ⸽⹜㛎ޠᐔᚑ19ᐕᐲᣣᧄ㉯ㅧቇળᄢળ⻠

Ṷⷐᣦ㓸p. 11㧘ർߣ߯޽ (2007ᐕ9᦬5ᣣ).

30) ᄢၔᣧᏗሶޔ๺૒㊁ᚑ੫ޔ᫪᧛ ⨃ޔḡ ጪℙޔᧁ↰ᑪᰴޟ὾㈪☺߆ࠄ⵾ㅧߒߚ㉯ㅧ

㈶ߩⴊዊ᧼ಝ㓸ᛥ೙૞↪ޠᐔᚑ 19 ᐕᐲᣣᧄ㉯ㅧቇળᄢળ⻠Ṷⷐᣦ㓸 p. 16㧘ർߣ߯޽

(2007ᐕ9᦬5ᣣ).

31)㑐 ቁᒄޔ᫪᧛ ⨃ޔᣇ ァޔ೨↰ ᶈޟࠢࡑࠗ╣㜞᷷㜞࿶᛽಴‛ߩ੃߇ࠎ⊒߇ࠎ੍㒐 ᵴᕈޠ߇ࠎ੍㒐ᄢળ in TOKYO 2007 (╙14࿁ᣣᧄ߇ࠎ੍㒐ቇળ), ቇⴚ✚ว࠮ࡦ࠲࡯㧔᧲

੩ㇺජઍ↰඙৻࠷ᯅ2-1-2㧕, ᐔᚑ19ᐕ7᦬12ᣣ㧔ᧁ㧕㨪13ᣣ㧔㊄㧕.

32)੉ഀ㓁ඳޔᑝ↰ᱜ৻㇢ޔ⑔↰㓶ౖޔḡ ጪℙޔ᫪᧛ ⨃ޔᧁ↰ᑪᰴޔჇ↰㦖຦ޔṚᎹ ᷡ ޟ᦭᣿ᶏᐓẟᐩᵆߦ߅ߌࠆᓸ↢‛ߩᯏ⢻ᕈㆮવሶࠍᮡ⊛ߣߒߚੱᎿᎽⓣ⸳⟎ലᨐߩ⹏

ଔޠ╙ ࿁ᣣᧄ᳓ⅣႺቇળᐕળ⻠Ṷⷐᣦ㓸 R,ᄢ㒋↥ᬺᄢቇᐔᚑ ᐕ ᦬ ᣣ 33)’ౝ⾆ሶޔ᧻᳗ᱜ⑲ޔ᳗↰ ⡡ޔᧃ᳗⾆ผޔự↰ᐽᄥ㇢ޔ๺૒㊁ᚑ੫ޔ᫪᧛ ⨃ޔᧁ

↰ᑪᰴޟ㤈὾㈪⫳⇐ᱷᷲ߆ࠄߩ㉯ㅧ㈶⵾ㅧᛛⴚߩታ⸽ޠ╙ ࿁ᾢᧄ⋵↥ቇቭᛛⴚ੤ᵹળ,

࠙ࠚ࡞ࠪ࠹ࠖᾢᧄ (2007ᐕ1᦬23ᣣ).

34)๺૒㊁ᚑ੫ޔᄢၔᣧᏗሶޔ᫪᧛ ⨃ޔḡ ጪℙޔᧁ↰ᑪᰴޔ’ౝ⾆ሶޔ᧻᳗ᱜ⑲ޔ᳗

↰ ⡡ޔᧃ᳗⾆ผޔự↰ᐽᄥ㇢ޔޟ㤈὾㈪⫳⇐ᱷᷲ߆ࠄ⵾ㅧߒߚ㉯ㅧ㈶ߩ↢ℂᵴᕈ⹏ଔޠ

╙ ࿁ᾢᧄ⋵↥ቇቭᛛⴚ੤ᵹળ, ࠙ࠚ࡞ࠪ࠹ࠖᾢᧄ (2007ᐕ1᦬23ᣣ).

(4) ࿑ᦠ

1) ࿑⸃ࡃࠗࠝࠛ࠲ࡁ࡯࡞⵾ㅧᛛⴚ㧦✬⠪␠࿅ᴺੱࠕ࡞ࠦ࡯࡞දળޔ

⊒ⴕᚲ Ꮏᬺ⺞ᩏળޔ2007ᐕ12᦬25ᣣ

╙㧡┨╙㧞▵ ♧Ᵽ⊒㉂ᑄᶧߩࡔ࠲ࡦ⊒㉂

ᾢᧄᄢቇᄢቇ㒮⥄ὼ⑼ቇ⎇ⓥ⑼Ꮏቇ♽ ᧁ↰ᑪᰴ

╙㧡┨╙㧟▵ ࠺ࡦࡊࡦ⾰⊒㉂ᑄᶧߩಣℂ߅ࠃ߮೑ᵴ↪

ᾢᧄᄢቇᄢቇ㒮⥄ὼ⑼ቇ⎇ⓥ⑼ ᫪᧛ ⨃

╙5┨╙㧠▵ ࠮࡞ࡠ࡯ࠬ♽ࡃࠗࠝࡑࠬߩ⊒㉂ᑄᶧಣℂ ᾢᧄᄢቇᄢቇ㒮⥄ὼ⑼ቇ⎇ⓥ⑼ ḡ ጪℙޔ቟ ᣿ື

ᐔᚑ ᐕᐲ⎇ⓥᚑᨐႎ๔

⎇ⓥ⺖㗴ฬ㧦ⅣႺᓸ↢‛ࠍᵴ↪ߔࠆ᳓࡮࿯ფⅣႺୃᓳ

ᚲዻ࡮᳁ฬ㧦⥄ὼ⑼ቇ⎇ⓥ⑼ ␠ળⅣႺᎿቇኾ᡹ Ꮉ⿧଻ᓼ

⎇ⓥ⋡⊛࡮⋡ᮡ

* ᐕᐲ㨪 ᐕᐲߦ߅ߌࠆ⋡⊛࡮⋡ᮡ

㜞ᐲᚑ㐳ᦼߦ߅ߌࠆ᳓ⅣႺߦ㑐ߔࠆ౏ኂ໧㗴ߪޔ㜞ᐲߦᳪỘߒߚ޽ࠆ޿ߪ᦭ኂߥൻ ቇ‛⾰ߦᳪᨴߐࠇߚ↥ᬺᑄ᳓ߦࠃߞߡᒁ߈⿠ߎߐࠇޔߘߩኻ╷ߦߟ޿ߡߪ޿߆ߦឃ಴

Ḯߢߘࠇࠍߊ޿ᱛ߼ࠆ߆߇ᦨ㊀ⷐ⺖㗴ߢ޽ߞߚޕߔߥࠊߜឃ᳓ಣℂᛛⴚࠍផㅴޔᔀᐩ ߔࠆߣߣ߽ߦޔឃ᳓᳓⾰ࡕ࠾࠲࡝ࡦࠣᛛⴚߩะ਄߇࿑ࠄࠇߚޕ੹ᣣߢߪޔߎߩࠃ߁ߥ 㐳ᐕߦᷰࠆឃ಴Ḯኻ╷߇ഞࠍᄼߒޔ߆ߟߡߩ౏ኂ໧㗴߇ౣ߮➅ࠅ㄰ߐࠇࠆߎߣߪߥ޿

ߣ⠨߃ࠄࠇࠆޕߒ߆ߒߥ߇ࠄ৻ᣇߢޔᣢߦᳪᨴߐࠇߚ᳓࡮࿯ფⅣႺ߳ߩኻ╷ߪ੹ߥ߅ ਇචಽߥ⁁ᴫߦ޽ࠆޕ․ߦ࿾ਅ᳓ਛߩ⓸⚛߿࡝ࡦߥߤߩᩕ㙃Ⴎ㘃߿ౝಽᴲᡬੂ‛⾰ߦ ઍ⴫ߐࠇࠆᓸ㊂ൻቇ‛⾰ߦࠃࠆ᳓ⅣႺᳪᨴߪޔߎࠇ߹ߢߩឃ಴Ḯኻ╷ߢߪኻಣߢ߈ߕޔ

዁᧪ߦᄢ߈ߥᓇ㗀ࠍ෸߷ߔᕟࠇ߽޽ࠆޕߎࠇࠄߦኻߒߡޔ↢‛Ꮏቇࠍᵴ↪ߔࠆᑄ᳓࡮

ᑄ᫈‛ಣℂᛛⴚ߿ⅣႺୃᓳᛛⴚ߇ߘߩኻ╷ߩ৻ߟߣߒߡᦼᓙߐࠇߡ޿ࠆޕᧄ⎇ⓥߢߪޔ

᦭↪ߥⅣႺᓸ↢‛ࠍᵴ↪ߒޔߢ߈ࠆߛߌ⃻႐ߦහᔕߒߚታ↪⊛ߥᑄ᳓࡮ᑄ᫈‛ಣℂޔ

ⅣႺୃᓳᛛⴚߩ㐿⊒ࠍ⋡⊛ߣߔࠆޕౕ૕⊛ߦߪޔᚒ߇࿖ߛߌߢߥߊࠕࠫࠕ߅ࠃ߮ో਎

⇇⊛ߦ໧㗴ߣߥߞߡ޿ࠆ⓸⚛ࠍߪߓ߼⒳ޘߩ᦭ኂൻቇ‛⾰ߦᳪᨴߐࠇߚᑄ᳓߿ⅣႺ

᳓ࠍޔૐࠦࠬ࠻ߢ㜞ㅦߦ㒰෰ߔࠆߚ߼ߩၮ␆ᛛⴚߩ⏕┙ࠍ⋡ᜰߔޕ

* ᐕᐲߦ߅ߌࠆ⋡⊛࡮⋡ᮡ

ᧄ⎇ⓥ⺖㗴ߦߟ޿ߡߪޔ* ᐕߦ⌕ᚻߒߡએ᧪ޔ࿾ਅ᳓ਛߩ⎣㉄࡮੝⎣㉄ᕈ⓸⚛ߩ 㒰෰ߦ㑐ߔࠆ⎇ⓥߦ៤ࠊߞߡ߈ߚޕ⓸⚛ߦࠃࠆஜᐽᓇ㗀ߣߒߡߪޔ੝⎣㉄⓸⚛߇ⴊᶧ ਛߩࡋࡕࠣࡠࡆࡦߣ⚿วߔࠆߎߣߢ㉄ᰳ⁁ᘒࠍᒁ߈⿠ߎߔޟࡔ࠻ࡋࡕࠣࡠࡆࡦⴊ∝ޠ ߇⍮ࠄࠇޔ☨࿖ߢߪ੃ఽߩᱫ੢଀߽޽ࠆޕ߹ߚޔ⓸⚛ߪ࡝ࡦߣߣ߽ߦޟᩕ㙃Ⴎ㘃ޠߣ ߒߡንᩕ㙃ൻ⃻⽎ߩේ࿃‛⾰ߢ޽ࠆߎߣߪࠃߊ⍮ࠄࠇߡ޿ࠆߣߎࠈߢ޽ࠆޕ

ⅣႺ᳓ޔ޽ࠆ޿ߪᑄ᳓߆ࠄߩ⓸⚛㒰෰ߦߟ޿ߡߪޔ↢‛ቇ⊛ಣℂᛛⴚ߇⍮ࠄࠇߡ߅ ࠅޔߎࠇߪਥߦࠕࡦࡕ࠾ࠕ߿᦭ᯏᘒ⓸⚛ࠍ⎣㉄޽ࠆ޿ߪ੝⎣㉄ߦ߹ߢ㉄ൻ㧔⎣ൻ㧕ߔ ࠆ⎣ൻ⚦⩶ߣޔߘߩㅒ෻ᔕޔߔߥࠊߜ⎣㉄߿੝⎣㉄ࠍ⓸⚛ࠟࠬߦㆶర㧔⣕⓸㧕ߔࠆ⣕

⓸⚦⩶ߩ௛߈ࠍ೑↪ߒߚ߽ߩߢ޽ࠆޕ↢‛ቇ⊛⓸⚛㒰෰ߪޔ⥄ὼ⇇ߦሽ࿷ߔࠆᓸ↢‛

ࠍ෻ᔕࠍ೑↪ߒޔട߃ߡ⓸⚛ࠍᄢ᳇ᚑಽߢ޽ࠆ⓸⚛ࠟࠬߦ߹ߢಣℂߢ߈ࠆߎߣ߆ࠄޔ ಣℂ᳓ࠍࠕ࡞ࠞ࡝ᕈߦߒߡᒝ೙ᦑ᳇ߔࠆߎߣߢࠕࡦࡕ࠾ࠕࠍើᢔߔࠆࠕࡦࡕ࠾ࠕࠬ

࠻࡝࠶ࡇࡦࠣᴺߥߤߩ‛ℂൻቇ⊛ಣℂߦᲧߴߡޔ޿ࠊࠁࠆ̌࿾⃿ߦఝߒ޿̍ᛛⴚߣ޿

߃ࠆޕߒ߆ߒߥ߇ࠄޔ⣕⓸ㆊ⒟ߢߪޔߘߩ෻ᔕࠍᜂ߁⣕⓸⚦⩶߇৻⥸ߦߪᓥዻᩕ㙃⚦

⩶ߢ޽ࠆߎߣ߆ࠄ὇⚛Ḯ߇ᔅⷐߣߥࠅޔಣℂ᳓ߩᕈ⁁ߦࠃߞߡߪޔ὇⚛Ḯߩᛩ౉ߦࠃ ࠆࠦࠬ࠻ߩჇᄢ߿ੑᰴᳪᨴߩ໧㗴߇ ᔨߐࠇࠆޕ

ߘߎߢޔᎹ⿧ߩ⎇ⓥࠣ࡞࡯ࡊߢߪޔ⣕⓸⚦⩶ߩ὇⚛Ḯߣߒߡ࿕૕⁁ߩ↢ಽ⸃ᕈࡊ࡜

ࠬ࠴࠶ࠢࠍᵴ↪ߔࠆ⎣㉄࡮੝⎣㉄ᕈ⓸⚛㒰෰ᛛⴚߩ⏕┙ࠍ⋡⊛ߣߒߡᬌ⸛ࠍⴕߞߡ߈ ߚޕ* ᐕᐲߦߪޔ↢ಽ⸃ᕈࡊ࡜ࠬ࠴࠶ࠢࠍలႯߒߚ࿯ფࠞ࡜ࡓߦࠃࠆ⎣㉄ᕈ⓸⚛ߩ ㆶర⣕⓸㒰෰ߦ㑐ߔࠆ⎇ⓥࠍታᣉߒޔ↢ಽ⸃ᕈࡊ࡜ࠬ࠴࠶ࠢߣߒߡޔࡐ࡝Ǫ㈼㉄

㧔RQN[ßJ[FTQZ[DWV[TCVGએਅ 2*$ ߣߔࠆ㧕ࠍ↪޿ߡㅪ⛯ታ㛎ࠍታᣉߒޔ቟ቯ࡮⦟

ᅢߥ⓸⚛㒰෰߇ᓧࠄࠇߚޕ* ᐕᐲߪޔ2*$ లႯ࿯ფࠞ࡜ࡓߦࠃࠆ⎣㉄ᕈ⓸⚛㒰෰ࡔ

ࠞ࠾࠭ࡓࠍ᣿ࠄ߆ߦߔࠆߚ߼ߦޔ⎣㉄ᕈ⓸⚛Ớᐲߣ࿯ფ⚦⩶ᢙ߿⚦⩶ฌߣߩ㑐ଥߦߟ

޿ߡ /20 ᴺޔ2%4&))' ᴺ╬ߩಽሶ↢‛ቇ⊛ᚻᴺࠍ↪޿ߡ⸃ᨆޔᬌ⸛ߒޔࠞ࡜ࡓౝㇱߢ ߩ⣕⓸⚦⩶ᢙ߅ࠃ߮⣕⓸ᵴᕈ߇ 2*$ లႯㇱ૏ߦߡᦨ߽㜞ߊޔߘߩਅᵹㇱߦ߅޿ߡ߽ห

⚦⩶ᢙ࡮ᵴᕈ߇⚻ᤨ⊛ߦჇᄢߔࠆߎߣߢ࡝ࠕࠢ࠲࡯ో૕ߣߒߡߩ⓸⚛㒰෰⢻߇ะ਄ߔ ࠆߎߣ߇᣿ࠄ߆ߣߥߞߚޕ߹ߚޔ⚦⩶ฌ⸃ᨆߩ⚿ᨐ߆ࠄޔ2*$ ട᳓ಽ⸃㉂⚛ࠍ᦭ߒޔ ߆ߟ⎣㉄ㆶర⢻߇⍮ࠄࠇࠆ 4CNUVQPKCRKEMGVVKK ߩㄭ✼⚦⩶߇ޔᧄ࡝ࠕࠢ࠲࡯ߩ⓸⚛

㒰෰ߦᄢ߈ߊነਈߒߡ޿ࠆߎߣ߇ផቯߐࠇߚޕߘߎߢ 㨪 ᐕᐲߪ 2*$ ࿯ფࠞ࡜ࡓ

࡝ࠕࠢ࠲࡯߆ࠄߩ 2*$ ⾗ൻᕈ⣕⓸⚦⩶ߩන㔌ࠍ⹜ߺޔࠢࠛࡦ㉄ࠍ὇⚛Ḯߣߒߡ⎣㉄⣕

⓸ࠍⴕ߁ࠦࡠ࠾࡯ࠍන㔌ߒߚޕᧄ⚦⩶ߪ 2*$ ☳ᧃࠍන৻὇⚛Ḯߣߔࠆ )KNVC[ ᡷᄌၭ

࿾਄ߦࠦࡠ࠾࡯ࠍ↢ᚑߒޔߘߩ๟ㄝߦߪ 2*$ ☳ᧃߩಽ⸃ࠍ␜ߔㅘ᣿ߥࡂࡠ࡯߇⹺߼ࠄ ࠇߚޕ߹ߚޔࠦࡠ࠾࡯ࠍਛᔃߣߒߡၭ࿾ో૕ߦ⎣㉄⣕⓸ߦࠃࠆ R* ਄᣹ࠍ␜ߔၭ࿾⦡

ߩ㕍ᄌ߇⹺߼ࠄࠇߚޕߐࠄߦޔ2*$ ࡍ࡟࠶࠻ࠍන৻὇⚛Ḯߣߔࠆ࠳࡯࡜ࡓ▤ߦࠃࠆ⣕

⓸⹜㛎ߢ㓁ᕈࠍ␜ߒߚޕએ਄߆ࠄޔන㔌⚦⩶ࠍ 2*$ ⾗ൻᕈ⣕⓸⚦⩶ߣ್ቯߒޔ5T&0#

㈩೉ߣ↢ൻቇ⹜㛎ࠃࠅ 4CNUVQPKCRKEMGVVK ߩㄭ✼⒳ߣផቯߒޔ-( ᩣ๮ฬߒߚޕ ߘߎߢ * ᐕᐲߪޔߪߓ߼ߦޔ࿁ಽታ㛎ࠍ➅ࠅ㄰ߒߥ߇ࠄ⎣㉄Ớᐲࠍ਄᣹ߐߖࠆߎ ߣߢޔ-( ᩣߩ⠴⎣㉄⢻߅ࠃ߮⣕⓸⢻ࠍ᣿ࠄ߆ߦߔࠆߎߣߣߒߚޕᰴߦޔᓸ↢‛ઃ⌕

ᜂ૕ߦਇ❱Ꮣࠍ↪޿ߡන㔌⚦⩶ࠍ଻ᜬߔࠆ࿕ቯᐥဳ࡝ࠕࠢ࠲ࠍ૞ᚑߒޔ2*$ ࠍ὇⚛Ḯ ߅ࠃ߮㔚ሶଏਈ૕ߣߔࠆㅪ⛯⎣㉄ᕈ⓸⚛㒰෰࡝ࠕࠢ࠲ߩ᭴▽ࠍᦨ⚳⋡ᮡߣߒߡᬌ⸛

ࠍⴕߞߚޕ

* ᐕᐲߩ⎇ⓥᚑᨐ

2*$ ࠍ㔚ሶଏਈ૕ߣߒߚ➅ࠅ㄰ߒ࿁ಽၭ㙃ߦࠃࠆ -( ᩣߩ⎣㉄⣕⓸⢻ߩ⹏ଔ * ᐕᐲߦߪޔࠢࠛࡦ㉄ࠍ὇⚛Ḯߣߒߡ࿁ಽታ㛎ࠍⴕ޿ޔ-( ߩ↢⢒߅ࠃ߮⎣㉄⣕

⓸⢻ߦ߅ࠃ߷ߔ᷷ᐲ߅ࠃ߮ R* ߦ㑐ߒߡᬌ⸛ߒߚޕߘߩ⚿ᨐޔ-( ᩣߪޔR*㨪ޔ 㨪͠ߩ᧦ઙਅߢ↢⢒น⢻ߢ޽ࠅޔ͠ߢޔR*㨪 ߩ᧦ઙਅߢᦨ߽⦟ᅢߥ↢⢒

ߣ㜞޿⎣㉄⣕⓸⢻ࠍ␜ߔߎߣ߇᣿ࠄ߆ߣߥߞߚޕ

ߘߎߢ * ᐕᐲߪޔR*㨪R* ߩ▸࿐ߢޔ2*$ ࠍ὇⚛Ḯ߅ࠃ߮㔚ሶଏਈ૕ߦޔ⎣

㉄ࠍ㔚ሶฃኈ૕ߣߒߡ -( ᩣߩ➅ࠅ㄰ߒ࿁ಽၭ㙃ታ㛎ࠍⴕߞߚޕ߹ߚޔ࿁ಽታ㛎ࠍ➅ ࠅ㄰ߔ㓙ޔᲑ㓏⊛ߦ⎣㉄Ớᐲࠍ਄᣹ߐߖޔ-( ᩣߩ⠴⎣㉄⢻߅ࠃ߮㒰෰㧔⣕⓸㧕⢻ࠍ

᣿ࠄ߆ߦߔࠆߎߣߣߒߚޕ

ドキュメント内 水環境汚染物質の動態評価研究拠点の構築[平成１ ９年度報告書] (ページ 39-50)