᪩✄⏣ᏛᏛ㝔 㐀⌮ᕤᏛ◊✲⛉
༤㻌 ኈ㻌 ㄽ㻌 ᩥ㻌 ᴫ㻌 せ
ㄽ ᩥ 㢟 ┠
㦵⏕་⒪⏝࠸ࡿ⏕యᮦᩱࡢ
⾲㠃ᛶ≧ࡢ᭱㐺㛵ࡍࡿ◊✲
The surface property optimizations of biomaterials used in bone tissue
engineering
⏦ ㄳ ⪅
⛬
Kan CHENG
ᆅ⌫࣭⎔ቃ㈨※⌮ᕤᏛᑓᨷ ᛂ⏝㖔≀◊✲
2012 ᖺ 12 ᭶
㏆ ᖺ 㸪 ே ᕤ 㦵 ࡸ ⤒ ⓶ ࢹ ࣂ ࢫ ࡢ స 〇 ࠶ ࡓ ࡾ 㸪 ⏕ య ᮦ ᩱ ⾲ 㠃 (⣽ ⬊ ࡢ
㊊ ሙ 㸪s c a f f o l d )ࡢ ᛶ ≧ ࡀ ⣽ ⬊ ࡢ ᥋ ╔ 㸪 ቑ Ṫ 㸪 ศ ࡞ ࡢ ᇶ ᮏ ⓗ ࡞ ⣽ ⬊ ᣲ
ື ࡁ ࡃ 㐃 ື ⓗ ᙳ 㡪 ࢆ ࠼ ࡿ ࡇ ࡀ ࢃ ࡗ ࡚ ࡁ ࡓ ࠋ ࡋ ࡋ ࡞ ࡀ ࡽ 㸪
⏕ య ᮦ ᩱ ୖ ࠾ ࡅ ࡿ ⣽ ⬊ ࡢ ᥋ ╔ ᣲ ື 㸪 ≉ ึ ᮇ ⣽ ⬊ ᥋ ╔ ᙧ ែ ㊊ ሙ ᛶ
≧ ࡢ 㛵 ಀ ࡣ ༑ ศ ゎ ᫂ ࡉ ࢀ ࡚ ࠸ ࡿ ࡣ ゝ ࠸ 㞴 ࠸ ࡢ ࡀ ⌧ ≧ ࡛ ࠶ ࡿ ࠋ ⌧ ᅾ 㸪⊂ ❧ ⾜ ᨻ ἲ ே ⏘ ᴗ ᢏ ⾡ ⥲ ྜ ◊ ✲ ᡤ (௨ ୗ ࡣ ⏘ ⥲ ◊ グ ࡍ )ࡢ ࣄ ࣗ ࣮ ࣐
ࣥ ࣛ ࣇ ࢸ ࢡ ࣀ ࣟ ࢪ ࣮ ◊ ✲ 㒊 㛛 ࡛ ࡣ ࠊ ⌧ ≧ ࡢ ⏕ ་ ⒪ ࡢ ⤒ ῭ ⓗ ᢏ ⾡ ⓗ ၥ 㢟 ࢆ Ⓨ ᒎ ⓗ ゎ Ỵ ࡍ ࡿ ᪉ ἲ ࡋ ࡚ ࠕ ⏕ య ෆ ᇙ ධ ࡍ ࡿ ᮦ ᩱ ⮬ ㌟ ࡀ
⣽ ⬊ ศ ࡸ ⤌ ⧊ ⏕ ࢆ ಁ ࡀ ࡍ ࠖ ➨ 3 ୡ ௦ ⏕ య ᮦ ᩱ ࡢ 㛤 Ⓨ ࢆ ࢸ ࣮ ࣐ ࡢ 㸯 ࡘ ࡋ ࡚ ◊ ✲ ࡀ ⾜ ࡞ ࢃ ࢀ ࡚ ࠸ ࡿ ࠋ ࠼ ࡤ 㸪 ⏘ ⥲ ◊ ࡛ ࡣ 8 0 ཬ ࡪ ᝈ
⪅ ⏤ ᮶ ࡢ 㛫 ⴥ ⣔ ᖿ ⣽ ⬊ ࢆ ⏝ ࠸ ࡚ ே ᕤ 㛵 ⠇ ࡞ ࡢ ⏕ య ᮦ ᩱ ୖ ࡛ ࡢ ᇵ 㣴 ࢆ
⾜ ࡗ ࡚ ⏕ ᇵ 㣴 㦵 ࢆ స 〇 ࡍ ࡿ ࡇ ᡂ ຌ ࡋ 㸪 㦵 ⏕ ་ ⒪ ࡢ ᗎ ᖥ ࢆ 㛤 ࠸ ࡓ ࡇ ࡀ ▱ ࡽ ࢀ ࡚ ࠸ ࡿ ࠋ ࡋ ࡋ ࡞ ࡀ ࡽ 㸪 ᝈ ⪅ ⏤ ᮶ ࡢ ⣽ ⬊ ࡣ ྠ ୍ ⤌ ⧊ ⏤ ᮶ ࡛ ࠶ ࡗ ࡚ ࡶ 㸪 ே ᕤ 㛵 ⠇ ࡞ ࡢ ⏕ య ᮦ ᩱ ୖ ࡛ ࡢ ⣽ ⬊ ศ ࡀ ࡁ ࡃ ␗ ࡞
ࡿ ࡇ ࡀ ☜ ㄆ ࡉ ࢀ ࡿ ୰ 㸪 ⏕ ᇵ 㣴 㦵 ࡀ ᙧ ᡂ ࡛ ࡁ ࡎ 㸪 ᝈ ⪅ ࡢ ⛣ ᳜ ⒪ ࡀ ฟ ᮶ ࡞ ࠸ ࢣ ࣮ ࢫ ࡶ ሗ ࿌ ࡉ ࢀ ࡚ ࠸ ࡿ ࠋ ࡇ ࡢ ≧ ἣ ࡽ 㸪 㦵 ⏕ ་ ⒪ ࡢ ᐇ
⏝ ࡢ ಁ 㐍 ࡣ 㸪 ⛣ ᳜ ᮇ ᚅ ࡉ ࢀ ࡿ 㦵 ᙧ ᡂ ⬟ ࢆ ᭷ ࡋ ࡚ ࠸ ࡿ ྰ ࢆ 㸪 ᇵ 㣴 ᪩ ᮇ ࡢ ⣽ ⬊ ᙧ ែ ࡼ ࡗ ࡚ ホ ౯ ࣭ ุ ᐃ ࡍ ࡿ ᢏ ⾡ ࡢ ☜ ❧ ࡀ ồ ࡵ ࡽ ࢀ ࡿ ࠋ
ࡃ 㸪 ⏕ య ᮦ ᩱ ୖ ࠾ ࡅ ࡿ ⣽ ⬊ ࡢ ึ ᮇ ᥋ ╔ ᙧ ែ ╔ ┠ ࡋ ࡚ ࠊ ⏕ య ᮦ
ᩱ ⾲ 㠃 ࡢ ᛶ ≧ ⣽ ⬊ ᣲ ື ࡢ ┦ 㛵 ࡀ ᫂ ࡽ ࡞ ࢀ ࡤ ࠊ 㦵 ⏕ ་ ⒪ ⏝ ࠸
ࡿ ⏕ య ᮦ ᩱ ࡢ ⣽ ⬊ ά ᛶ Ᏻ ᐃ ᭱ 㐺 ࡁ ࡃ ㈉ ⊩ ࡍ ࡿ ࡇ ࡀ ᮇ ᚅ ࡉ
ࢀ ࡿ ࠋ ࡑ ࡇ ࡛ ᮏ ◊ ✲ ࡛ ࡣ 㸪 㦵 ⏕ ་ ⒪ ࡛ ỗ ⏝ ⏝ ࠸ ࡽ ࢀ ࡿ ࢭ ࣛ ࣑ ࢵ ࢡ ࢫ ⣔ ࣭ 㔠 ᒓ ⣔ ⏕ య ᮦ ᩱ ࡋ ࡚ 㸪 ( 1 ) 㠀 ྾ ᛶ ș - T C P ྵ ᭷ ࣃ ࢱ ࢺ 㸪
( 2 ) ␗ ࡞ ࡿ ᚤ ⣽ ᵓ 㐀 ࢆ ᭷ ࡍ ࡿ ࣃ ࢱ ࢺ 㸪 ( 3 ) ✀ ࠎ ࡢ ࣃ ࢱ ࢺ ┦ ࡛ ⾲
㠃 ಟ 㣭 ࡋ ࡓ ࢳ ࢱ ࣥ ྜ 㔠 㸪 ࡢ 㸱 ✀ 㢮 ࡢ ࢹ ࢫ ࢡ ࢆ స 〇 ࡋ 㸪 ࡇ ࢀ ࡽ ⏕ య ᮦ
ᩱ ࡢ ᭱ 㐺 ࡞ ⾲ 㠃 ᛶ ≧ ࢆ ẚ ㍑ ᳨ ウ ࡋ ࠊ ␗ ࡞ ࡿ ⏕ య ᮦ ᩱ ୖ ࠾ ࡅ ࡿ ⣽ ⬊ ᣲ
ື ࡢ ண ᢏ ⾡ ࡢ 㛤 Ⓨ ࢆ ┠ ⓗ ࡋ ࡓ ࠋ ᮏ ㄽ ᩥ ࡣ 㸪 㸳 ❶ ࡼ ࡾ ᵓ ᡂ ࡉ ࢀ ࡚ ࠸ ࡿ ࠋ
➨ 㸯 ❶ ࡛ ࡣ 㸪 ᮏ ㄽ ᩥ ࡢ ◊ ✲ ⫼ ᬒ ᴫ せ 㸪 ࡃ ◊ ✲ ⏝ ࠸ ࡓ ᐇ 㦂 ᪉ ἲ ࡘ ࠸ ࡚ ㏙ ࡚ ࠸ ࡿ ࠋ
ල య ⓗ ⏕ య ᮦ ᩱ ࡑ ࡢ ⾲ 㠃 ࡛ ࡢ ⣽ ⬊ ᣲ ື ࡢ ┦ 㛵 ࢆ ᢕ ᥱ ࡍ ࡿ ࡓ ࡵ
ࡣ 㸪 ᙜ ヱ ⏕ య ᮦ ᩱ ୖ ࠾ ࡅ ࡿ ⣽ ⬊ ࡢ ึ ᮇ ᥋ ╔ ᙧ ែ ࢆ ᢕ ᥱ ࡋ 㸪 ࡉ ࡽ ࡑ ࡢ ึ ᮇ ⣽ ⬊ ᥋ ╔ ᙧ ែ ⣽ ⬊ ศ ⬟ ࡢ ┦ 㛵 ᛶ ࢆ ゎ ᫂ ࡍ ࡿ ࡇ ࡀ ᚲ せ ࡛
࠶ ࡿ ࠋ ࡃ 㸪 ⏕ య ᮦ ᩱ ୖ ࠾ ࡅ ࡿ ⣽ ⬊ ࡢ ึ ᮇ ᥋ ╔ ᙧ ែ ࢆ ㄪ ࡿ ࡓ ࡵ
ࡣ 㸪 ึ ᮇ ⣽ ⬊ ᥋ ╔ ᙧ ែ ࡢ ྍ ど ᢏ ⾡ ホ ౯ ᢏ ⾡ ࡀ ồ ࡵ ࡽ ࢀ ࡿ ࠋ ⏕ య
ᮦ ᩱ ୖ ࡛ ࡢ ึ ᮇ ⣽ ⬊ ᥋ ╔ ᙧ ែ ࡢ ྍ ど ᢏ ⾡ ࡋ ࡚ 㸪 ࡇ ࢀ ࡲ ࡛ ࡢ ⣽ ⬊ ⏕
≀ Ꮫ ࡛ ࡣ 㸪 ㏱ ᫂ ࡞ ᮦ ᩱ ୖ ᇵ 㣴 ࡋ ࡓ ⣽ ⬊ ࢆ ┦ ᕪ 㢧 ᚤ 㙾 ࡛ ほ ᐹ ࡍ ࡿ ᪉
ἲ ࡀ ୍ ⯡ ⓗ ࡛ ࠶ ࡿ ࡀ 㸪 ᮏ ㄽ ᩥ ࡛ ࡣ 㔠 ᒓ ⣔ ࣭ ࢭ ࣛ ࣑ ࢵ ࢡ ࢫ ⣔ ࡞ ࡢ ㏱
᫂ ࡞ ⏕ య ᮦ ᩱ ࢆ ⏝ ࡍ ࡿ ࡓ ࡵ 㸪 ㏱ 㐣 ᆺ ࡢ ග Ꮫ ⣔ ࢆ ⏝ ࠸ ࡿ ┦ ᕪ 㢧 ᚤ 㙾
࡛ ࡣ ほ ᐹ ࡣ ྍ ⬟ ࡛ ࠶ ࡿ ࠋ ࡑ ࡇ ࡛ 㸪 ࡲ ࡎ ㏱ ᫂ ࡞ ⏕ య ᮦ ᩱ ୖ ᥋ ╔ ࡋ ࡓ ⣽ ⬊ ࢆ ྍ ど ࡍ ࡿ ࡇ ࢆ ヨ ࡳ ࡓ ࠋ ࡑ ࡢ ୍ ࡋ ࡚ 㸪 ⏕ ⣽ ⬊ ࢆ ⺯ ග ≀
㉁ ࡼ ࡗ ࡚ ᰁ Ⰽ ࡍ ࡿ ᢏ ⾡ ࡼ ࡾ 㸪 ⏕ య ᮦ ᩱ ᥋ ╔ ࡋ ࡓ ⣽ ⬊ ࢆ ྍ ど ࡍ
ࡿ ほ ᐹ ᢏ ⾡ ࢆ ☜ ❧ ࡋ ࡓ ࠋ ࡲ ࡓ 㸪 ⏕ య ᮦ ᩱ ୖ ࡛ ࡢ ึ ᮇ ⣽ ⬊ ᥋ ╔ ᙧ ែ ࡢ ホ ౯ ᢏ ⾡ ࡋ ࡚ ỗ ⏝ ࡢ ྍ ど ᢏ ⾡ ࢆ ᛂ ⏝ ࡋ ࠊ ⏕ య ᮦ ᩱ ୖ ᥋ ╔ ࡋ ࡓ ⣽ ⬊ ࡢ ึ ᮇ ᙧ ែ ࢆ ⏬ ീ ࡍ ࢀ ࡤ 㸪 ⏬ ീ ฎ ⌮ ࢯ ࣇ ࢺ ࢆ ⏝ ࠸ ࡓ ゎ ᯒ ࡣ ྍ ⬟ ࡞
ࡿ ⪃ ࠼ ࡓ ࠋ ࡑ ࡇ ࡛ ᮏ ◊ ✲ ࡛ ࡣ 㸪 ” i m a g e - p r o ” ࠸ ࠺ ⣽ ⬊ ᙧ ែ ⏬ ീ ฎ ⌮ ᑓ ⏝ ࡢ ᕷ ㈍ ࢯ ࣇ ࢺ ࢆ ⏝ ࡋ ࡚ 㸪 ⏕ య ᮦ ᩱ ᥋ ╔ ࡋ ࡓ ึ ᮇ ⣽ ⬊ ࡘ ࠸ ࡚ ᙧ ែ (㠃 ✚ )࡞ ࡢ ࣃ ࣛ ࣓ ࣮ ࢱ ࢆ ⮬ ື ⓗ ᩘ ್ ࡍ ࡿ ᪉ ἲ ࢆ ⏝ ࠸ ࡓ ࠋ
ᮏ ◊ ✲ ࡛ 㛤 Ⓨ ࡋ ࡓ ホ ౯ ᢏ ⾡ ࡢ Ⅼ ࡋ ࡚ ࡣ 㸪 ձ ᧯ స ࡀ ⡆ ༢ 㸪 ղ 1 0 0 ಶ ௨ ୖ ࡢ ⣽ ⬊ ࡀ 㐃 ⥆ ⓗ ほ ᐹ ࡛ ࡁ ࡿ ࡇ ࡛ 㸪 ホ ౯ ⤖ ᯝ ࡢ ୍ ⯡ ࡀ ࡛ ࡁ
ࡿ 㸪 ࡞ ࡀ ࠶ ࡿ ࠋ ୍ ᪉ 㸪 ᮏ ホ ౯ ᢏ ⾡ ࡢ ᙅ Ⅼ ࡋ ࡚ 㸪 ձ ⣽ ⬊ ࡢ ᢞ ᙳ 㠃 ✚
ࢆ ほ ᐹ ࡍ ࡿ ࡓ ࡵ 㸪 ⏕ య ᮦ ᩱ ࡢ ⾲ 㠃 ≧ ែ ᐃ ್ ࡀ ᕥ ྑ ࡉ ࢀ ࡿ 㸪 ղ 㠃 ✚
⏬ ീ ࡢ ⥡ 㯇 ࡉ ࡣ ⣽ ⬊ ཌ ࡳ ᕥ ྑ ࡉ ࢀ ࡿ 㸪 ࡞ ࡀ ࠶ ࡿ ࠋ ࡉ ࡽ ᮏ ホ ౯ ᢏ
⾡ ࢆ ᛂ ⏝ ࡋ ࡚ 㸪 ⏕ య ᮦ ᩱ ࡢ ⾲ 㠃 ᛶ ≧ ⾲ 㠃 ୖ ࠾ ࡅ ࡿ ᵝ ࠎ ࡞ ⣽ ⬊ ᣲ ື
ࡢ ┦ 㛵 ᛶ ࢆ ồ ࡵ ࢀ ࡤ 㸪 ึ ᮇ ⣽ ⬊ ᥋ ╔ 㠃 ✚ ࡼ ࡿ ⣽ ⬊ ศ ࡢ ண ᢏ ⾡
ࡢ ᛂ ⏝ ࡀ ᮇ ᚅ ࡛ ࡁ ࡿ ⪃ ࠼ ࡽ ࢀ ࡿ ࠋ ࡑ ࡇ ࡛ 㸪 ᐇ 㝿 ᩘ ✀ ࡢ 㔠 ᒓ ⣔ ࣭ ࢭ ࣛ ࣑ ࢵ ࢡ ࢫ ⣔ ⏕ య ᮦ ᩱ ࢆ స 〇 ࡋ 㸪 ࡑ ࢀ ࡽ ࡢ ⾲ 㠃 ຍ ⇕ ฎ ⌮ ࡸ 㓟 ฎ ⌮
➼ ࡢ ≀ ⌮ ⓗ 㸪 Ꮫ ⓗ ฎ ⌮ ࢆ ࡍ ࡇ ࡼ ࡗ ࡚ ⾲ 㠃 ᛶ ≧ ࢆ ㄪ ᩚ ࡋ 㸪 ࡑ ࡢ
ୖ ࡛ ⣽ ⬊ ࢆ ᇵ 㣴 ࡋ ࡚ ึ ᮇ ⣽ ⬊ ᙧ ែ ⣽ ⬊ ศ ⬟ ࡢ ኚ ┦ 㛵 ᛶ ࢆ ⣔ ⤫
ⓗ ㄪ ࡿ ᪉ ἲ ࡢ ᵓ ⠏ ࡘ ࠸ ࡚ ㏙ ࡚ ࠸ ࡿ ࠋ
➨ 2 ❶ ࡛ ࡣ 㸪ࠕ 㠀 ྾ ᛶ ș - T C P ྵ ᭷ ࣃ ࢱ ࢺ ࢹ ࢫ ࢡ ୖ ࠾ ࡅ ࡿ
⣽ ⬊ ึ ᮇ ᥋ ╔ ᣲ ື ཬ ࡧ ⣽ ⬊ ศ ⬟ ࡢ ┦ 㛵 ࠖ ࡘ ࠸ ࡚ ㏙ ࡚ ࠸ ࡿ ࠋ ᮏ ❶ ࡛ ࡣ ࡲ ࡎ 㸪 ࢭ ࣛ ࣑ ࢵ ࢡ ࢫ ⣔ ⏕ య ᮦ ᩱ ࡋ ࡚ Ỉ 㓟 ࣃ ࢱ ࢺ ⢊ య
ș 7 & 3 ⢊ య ࢆ ␗ ࡞ ࡿ ྜ ࡛ ΰ ྜ ࡋ 㸪 ᡤ ᐃ ᗘ ࡛ ↝ ᡂ ࡋ ࡚ 㸪 ᵝ ࠎ ࡞ ᚤ
⣽ ᵓ 㐀 ࢆ ᭷ ࡍ ࡿ 㠀 ྾ ᛶ ș 7 & 3 ྵ ᭷ ࣃ ࢱ ࢺ ࢹ ࢫ ࢡ ࢆ స 〇 ࡍ ࡿ ᡭ ἲ ࢆ ☜ ❧ ࡋ ࡓ ࠋ ࡑ ࡋ ࡚ 㸪 ࡃ ୍ ⯡ ་ ⏝ ᮦ ᩱ ࡋ ࡚ ⏝ ࠸ ࡽ ࢀ ࡚ ࠸ ࡿ ΰ ྜ ㄪ 〇 ẚ ࡢ 㠀 ྾ ᛶ ș 7 & 3 ྵ ᭷ ࣃ ࢱ ࢺ ࢹ ࢫ ࢡ ୖ ࠾ ࠸ ࡚ ࣛ ࢵ ࢺ ⏤ ᮶ 㛫 ⴥ ⣔ ᖿ ⣽ ⬊ ࢆ ᇵ 㣴 ࡋ ࡓ 㝿 ࡢ ึ ᮇ ᥋ ╔ 㠃 ✚ ࡢ ྍ ど ᢏ ⾡ ࣭ ホ ౯ ᢏ ⾡ ࢆ ☜ ❧ ࡋ 㸪 ⣽ ⬊ ࡢ ึ ᮇ ᥋ ╔ 㠃 ✚ ཬ ࡧ ศ ⬟ ࡢ ┦ 㛵 ᛶ ࡘ ࠸ ࡚ ㄪ ࡓ ࠋ ࡑ ࡢ ⤖ ᯝ 㸪 ࢹ ࢫ ࢡ ⾲ 㠃 ࡢ ș 7 & 3 ⢏ Ꮚ ࠾ ࡼ ࡧ ࣁ ࢻ ࣟ ࢟ ࢩ ࣃ ࢱ
ࢺ ⢏ Ꮚ ࡢ ⤖ ᬗ ⢏ ᚄ ࠾ ࡼ ࡧ 㞟 ྜ ⤌ ⧊ ᛶ ≧ ࡀ 㸪 ⾲ 㠃 ࡛ ᇵ 㣴 ࡋ ࡓ ࣛ ࢵ ࢺ ⣽
⬊ ࡢ ึ ᮇ ᥋ ╔ 㠃 ✚ ཬ ࡧ ศ ⬟ ᭷ ព ᙳ 㡪 ࢆ ࠼ ࡿ ࡇ ࢆ ぢ ฟ ࡋ ࡓ ࠋ ࡇ ࢀ ࡼ ࡾ 㸪 㠀 ྾ ᛶ ș 7 & 3 ྵ ᭷ ࣃ ࢱ ࢺ ㉁ ᮦ ᩱ ࡢ ᭱ 㐺 ࡞ ⾲ 㠃 ㄪ 〇 ᮲ ௳ ࢆ ồ ࡵ ࡿ ࡇ ࡀ ࡛ ࡁ ࡓ ࠋ
➨ 3 ❶ ࡛ ࡣ 㸪ࠕ ␗ ࡞ ࡿ ᚤ ⣽ ᵓ 㐀 ࢆ ᭷ ࡍ ࡿ ࣃ ࢱ ࢺ ࢹ ࢫ ࢡ ୖ ࠾ ࡅ
ࡿ ⣽ ⬊ ึ ᮇ ᥋ ╔ ᣲ ື ཬ ࡧ ⣽ ⬊ ศ ⬟ ࡢ ┦ 㛵 ࠖ ࡘ ࠸ ࡚ ㏙ ࡚ ࠸ ࡿ ࠋ ᮏ ❶ ࡛ ࡣ 㸪 ࢭ ࣛ ࣑ ࢵ ࢡ ࢫ ⣔ ⏕ య ᮦ ᩱ ࡋ ࡚ ࣁ ࢻ ࣟ ࢟ ࢩ ࣃ ࢱ ࢺ
⢊ య ࢆ ཎ ᩱ ࡋ ࡚ ⏝ ࠸ ࠊ ␗ ࡞ ࡿ ↝ ⤖ ᗘ ࡼ ࡾ ࠊ ᵝ ࠎ ࡞ ⤖ ᬗ ⢏ ᚄ ศ ᕸ
⾲ 㠃 ᚤ ⣽ ᵓ 㐀 ࢆ ᭷ ࡍ ࡿ ࣃ ࢱ ࢺ ࢹ ࢫ ࢡ ࢆ ⣔ ⤫ ⓗ స 〇 ࡍ ࡿ ᡭ ἲ
ࢆ ☜ ❧ ࡋ ࡓ ࠋ ࡑ ࡋ ࡚ 㸪 ࡑ ࢀ ࡽ ⾲ 㠃 ᛶ ≧ ࡢ ␗ ࡞ ࡿ ࣁ ࢻ ࣟ ࢟ ࢩ ࣃ ࢱ ࢺ ࢹ ࢫ ࢡ ୖ ࣛ ࢵ ࢺ ⏤ ᮶ ࡢ 㛫 ⴥ ⣔ ᖿ ⣽ ⬊ ࢆ ✀ ࡋ 㸪 㸱 㐌 㛫 ᇵ 㣴 ᚋ ࡢ
᪩ ᮇ ࡢ ⣽ ⬊ ᥋ ╔ 㠃 ✚ 㦵 ᙧ ᡂ ศ ࡢ ┦ 㛵 ࢆ ☜ ㄆ ࡋ ࡓ ࠋ ࢹ ࢫ ࢡ ⾲ 㠃 ࡢ
ࣁ ࢻ ࣟ ࢟ ࢩ ࣃ ࢱ ࢺ ⢏ Ꮚ ࢧ ࢬ ⣽ ⬊ ᥋ ╔ 㠃 ✚ ࡢ 㛫 ࡛ 㧗 ࠸ ┦ 㛵 ᛶ ࡀ ㄆ ࡵ ࡽ ࢀ ࡓ ࠋ ࡃ Υ ↝ ᡂ ࡛ స 〇 ࡋ ࡓ ࢹ ࢫ ࢡ ࡛ ࡣ 㸪 ⾲ 㠃 ࡣ ⤖ ᬗ ⢏ Ꮚ ᚄ ࡀ ᖹ ᆒ 㸯 㹼 㸰 ȣ P ࡢ ⦓ ᐦ 㞟 ྜ ⤌ ⧊ ࡞ ࡗ ࡓ ࡀ 㸪⣽ ⬊ ᥋ ╔ 㠃 ✚ ࡀ
᭷ ព ᑠ ࡉ ࡃ ࡞ ࡾ 㸪 ᑐ ᛂ ࡋ ࡚ ࢜ ࢫ ࢸ ࢜ ࢝ ࣝ ࢩ ࣥ ⏕ ᡂ 㔞 ࡀ ከ ࡃ ࡞ ࡗ ࡓ ࡇ
ࡽ 㸪 ᮏ ᐇ 㦂 ⣔ ࡛ ࡣ 㦵 ศ ά ᛶ ࡀ ᭱ ࡶ 㧗 ࡃ ࡞ ࡿ ࡇ ࡀ ࢃ ࡗ ࡓ ࠋ
➨ 4 ❶ ࡛ ࡣ 㸪ࠕ ✀ ࠎ ࡢ ࣃ ࢱ ࢺ ᒙ ࡛ ⾲ 㠃 ಟ 㣭 ࡋ ࡓ ࢳ ࢱ ࣥ ྜ 㔠 ୖ ࠾ ࡅ
ࡿ ⣽ ⬊ ึ ᮇ ᥋ ╔ ᣲ ື ཬ ࡧ ⣽ ⬊ ศ ⬟ ࡢ ┦ 㛵 ࠖ ࡘ ࠸ ࡚ ㏙ ࡚ ࠸ ࡿ ࠋ ᮏ ❶ ࡛ ࡣ 㸪 ୍ ⯡ ே ᕤ 㦵 ࡸ ே ᕤ ṑ ᰿ ࡋ ࡚ ⏝ ࠸ ࡽ ࢀ ࡿ 㔠 ᒓ 㸭 ࢭ ࣛ ࣑
ࢵ ࢡ ࢫ ࡢ 」 ྜ ⏕ య ᮦ ᩱ ࡋ ࡚ 㸪 ࢳ ࢱ ࣥ ྜ 㔠 ᇶ ᮦ ࡢ ⾲ 㠃 ᵝ ࠎ ࡞ ᮲ ௳ ࡛ ຍ ⇕ ฎ ⌮ ࡸ 㓟 ฎ ⌮ ࢆ ࡋ 㸪 ࡉ ࡽ ࡑ ࡢ ୖ ✀ ࠎ ࡢ ⤖ ᬗ ┦ ⤌ ᡂ ࡸ ᚤ ⣽ ᵓ 㐀 ࢆ ᭷ ࡋ ࡓ ࣃ ࢱ ࢺ ᒙ ࢆ ຠ ⋡ ⓗ ಟ 㣭 ᙧ ᡂ ࡍ ࡿ ᡭ ἲ ࢆ ☜ ❧ ࡋ ࡓ ࠋ ᙧ ᡂ ࡉ ࢀ ࡓ ࣃ ࢱ ࢺ ಟ 㣭 ᒙ ࡣ ᇶ ᮏ ⓗ ࡣ ࢳ ࢱ ࣥ ྜ 㔠 ⾲ 㠃 ࡢ 㓟 ≧ ែ
ࡼ ࡿ ᛶ ≧ ࡢ ኚ ࡼ ࡗ ࡚ 㸪 ࣃ ࢱ ࢺ ᒙ ࡢ & D 3 ẚ ࡞ ࡢ ⤌ ᡂ ࡸ ᚤ ⣽ ᵓ 㐀 ࡀ ᭷ ព ኚ ࡋ ไ ᚚ ࡉ ࢀ ࡿ ࡇ ࡀ ࢃ ࡗ ࡓ ࠋ స 〇 ࡋ ࡓ ✀ ࠎ ࡢ ࣃ ࢱ
ࢺ ᒙ ࡛ ⾲ 㠃 ಟ 㣭 ࡋ ࡓ ࢳ ࢱ ࣥ ྜ 㔠 〇 ᇶ ᯈ ࡸ ⤒ ⓶ ࢹ ࣂ ࢫ ⏝ ࡢ ࣆ ࣥ ࡢ ୖ
࡛ ࣛ ࢵ ࢺ ⏤ ᮶ ࡢ 㛫 ⴥ ⣔ ᖿ ⣽ ⬊ ࢆ ᇵ 㣴 ࡋ ࡓ ࡇ ࢁ 㸪 ୖ グ ᐇ 㦂 ྠ ᵝ ึ ᮇ ᥋ ╔ ࠾ ࡅ ࡿ ⣽ ⬊ ᙧ ែ ⣽ ⬊ ศ ࡢ ┦ 㛵 ᛶ ࢆ ☜ ㄆ ࡛ ࡁ ࡓ ࠋ
➨ 5 ❶ ࠕ ⤖ ㄽ ࠖ
ᮏ ❶ ࡛ ࡣ 㸪 ᮏ ㄽ ᩥ ࡢ ᡂ ᯝ ࢆ ࡲ ࡵ 㸪 ࡉ ࡽ ⏕ య ᮦ ᩱ ୖ ࡛ ࡢ ⣽ ⬊ ศ ࡢ ண ᢏ ⾡ ࡞ ࡢ ᛂ ⏝ ᒎ 㛤 ࡢ ྍ ⬟ ᛶ ࡘ ࠸ ࡚ ㏙ ࡚ ࠸ ࡿ ࠋ
㹌㹭
᪩✄⏣Ꮫ ༤ኈ㸦ᕤᏛ㸧 Ꮫ⏦ㄳ ◊✲ᴗ⦼᭩
Ặྡ ⛬ ༳
㸦 ᖺ ᭶ ⌧ᅾ㸧
✀㢮ู 㢟ྡࠊ Ⓨ⾲࣭Ⓨ⾜ᥖ㍕ㄅྡࠊ Ⓨ⾲࣭Ⓨ⾜ᖺ᭶ࠊ 㐃ྡ⪅㸦⏦ㄳ⪅ྵࡴ㸧
➹㢌ㄽᩥ
ᰝㄞ᭷
➹㢌ㄽᩥ
ᰝㄞ᭷
ඹ ⴭ ㄽ ᩥ ᰝㄞ᭷
ᏛⓎ⾲
ᏛⓎ⾲
ᏛⓎ⾲
ᏛⓎ⾲
ࠐ Correlation between cell attachment areas after 2 hours of culture and osteogenic
differentiation activity of rat mesenchymal stem cells on hydroxyapatite substrates with various surface
Kan Cheng, Motohiro Hirose, Xiupeng Wang, Yu Sogo, Atsushi Yamazaki, Atsuo Ito Biochemical and Biophysical Research Communications 2012.
ࠐ Development of an early estimation method for predicting later osteogenic differentiation activity of rat mesenchymal stem cells cultured on biomaterials
Kan Cheng, Motohiro Hirose, Xiupeng Wang, Yu Sogo, Atsushi Yamazaki, Atsuo Ito Science and Technology of Advanced Materials 2012.
Effect of coprecipitation temperature on the properties and activity of fibroblast growth factor-2 apatite composite layer
Xia Li, Xiupeng Wang, Ito Atsuo, Sogo Yu, Kan Cheng, Oyane Ayako Materials Science and Engineering C 2009, vol.29, 216-221
ᐊ 2 ᅇἲࡼࡿ㝧ᴟ㓟ࢳࢱࣥࣆࣥୖࡢ⧄⥔ⱆ⣽⬊ᡂ㛗ᅉᏊ(FGF)-2/ࣃࢱࢺ」
ྜᒙࡢᙧᡂ
⛬ࠊᒣ㷂῟ྖࠊ▮᰿⥤Ꮚࠊ༑Ἑࠊ⋤⚽㭉ࠊఀ⸨ᩔኵ 2008ᖺ3᭶ ᪥ᮏࢭ࣑ࣛࢵࢡࢫ༠2008ᖺᖺ
FGF-2-ࣃࢱࢺ」ྜᒙࡢTiࣆࣥୖࡢᙧᡂ
⛬ࠊᒣ㷂῟ྖࠊ▮᰿⥤Ꮚࠊ༑Ἑࠊ⋤⚽㭉ࠊఀ⸨ᩔኵ 2008ᖺ11᭶ ᪥ᮏࣂ࣐࢜ࢸࣜࣝᏛࢩ࣏ࣥ࢘ࢪ࣒࢘2008
✀ࠎࡢࣃࢱࢺᒙ࡛⾲㠃ಟ㣭ࡋࡓࢳࢱࣥୖ࠾ࡅࡿ⣽⬊ᣲືࡢホ౯
⛬ࠊᘅ℩ᚿᘯࠊභᓮ⿱㧗ࠊ⋤⚽㭉ࠊ༑Ἑࠊᒣ㷂῟ྖࠊఀ⸨ᩔኵ 2010ᖺ11᭶ ➨32ᅇ᪥ᮏࣂ࣐࢜ࢸࣜࣝᏛ
✀ࠎࡢ⾲㠃ᛶ≧ࢆ᭷ࡍࡿࣃࢱࢺᒙ」ྜయࢆ⏝࠸ࡓ㛫ⴥ⣔ᖿ⣽⬊ᇵ㣴
⛬ࠊᘅ℩ᚿᘯࠊභᓮ⿱㧗ࠊ⋤⚽㭉ࠊ༑Ἑࠊᒣ㷂῟ྖࠊఀ⸨ᩔኵ
2011ᖺ3᭶ ➨10ᅇ᪥ᮏ⏕་⒪Ꮫ⥲
㹌㹭
᪩✄⏣Ꮫ ༤ኈ㸦ᕤᏛ㸧 Ꮫ⏦ㄳ ◊✲ᴗ⦼᭩
✀㢮ู 㢟ྡࠊ Ⓨ⾲࣭Ⓨ⾜ᥖ㍕ㄅྡࠊ Ⓨ⾲࣭Ⓨ⾜ᖺ᭶ࠊ 㐃ྡ⪅㸦⏦ㄳ⪅ྵࡴ㸧
ᏛⓎ⾲
ᏛⓎ⾲
ᏛⓎ⾲
ᏛⓎ⾲
Correlation between osteoblastic differentiation activity and cell morphology of rat mesenchymal stem cells
Kan Cheng, Motohiro Hirose, Hirotaka Mutsuzaki, Xiupeng Wang, Yu Sogo, Atsushi Yamazaki, Atsuo Ito
2011ᖺ3᭶ᅜ㝿ᖿ⣽⬊Ꮫ
ᚤ⣽⾲㠃ᛶ≧ࢆ᭷ࡍࡿࢳࢱࣥࣃࢱࢺᒙ」ྜయࢆ⏝࠸ࡓ㛫ⴥ⣔ᖿ⣽⬊ࡢ㦵ศホ౯
⛬ࠊᘅ℩ᚿᘯࠊභᓮ⿱㧗ࠊ⋤⚽㭉ࠊ༑Ἑࠊᒣ㷂῟ྖࠊఀ⸨ᩔኵ 2011ᖺ11᭶ ➨33ᅇ᪥ᮏࣂ࣐࢜ࢸࣜࣝᏛ
㦵ࡢ⏕་⒪⏝࠸ࡿ⏕యᮦᩱࡢ᭱㐺࡞⾲㠃ᛶ≧ࡢゎ᫂ࡑࡢձ
⛬ࠊᘅ℩ᚿᘯࠊභᓮ⿱㧗ࠊ⋤⚽㭉ࠊ༑Ἑࠊᒣ㷂῟ྖࠊఀ⸨ᩔኵ
2012ᖺ1᭶ࡘࡃࡤ་ᕤ㐃ᦠࣇ࢛࣮࣒ࣛ ࠕ་ᕤ㐃ᦠ◊✲ࡽ♫㑏ඖ࣭⏘ᴗࠖ
㦵ࡢ⏕་⒪⏝࠸ࡿ⏕యᮦᩱࡢ᭱㐺࡞⾲㠃ᛶ≧ࡢゎ᫂ࡑࡢղ
⛬ࠊᘅ℩ᚿᘯࠊ⋤⚽㭉ࠊ༑Ἑࠊᒣ㷂῟ྖࠊఀ⸨ᩔኵ 2012ᖺ11᭶ ᪥ᮏࣂ࣐࢜ࢸࣜࣝᏛࢩ࣏ࣥ࢘ࢪ࣒࢘
㹌㹭
᪩✄⏣Ꮫ ༤ኈ㸦ᕤᏛ㸧 Ꮫ⏦ㄳ ◊✲ᴗ⦼᭩
✀㢮ู 㢟ྡࠊ Ⓨ⾲࣭Ⓨ⾜ᥖ㍕ㄅྡࠊ Ⓨ⾲࣭Ⓨ⾜ᖺ᭶ࠊ 㐃ྡ⪅㸦⏦ㄳ⪅ྵࡴ㸧
