Miller unit

Fig. 4-14 nuoA lacZ × 3(A) nuoA 3(B) nuoA

× WT

∆arcA

LacZ 3 3

3

Fig. 4-15 Δ Δ MR-1 3Fdh: formate

dehydrogenase 2 Nuo: NADH dehydrogenase 2 c (FccA

CctA)

電極 

Nuo ^QH_Q²

CymA e

-e

-ArcS

ArcA

nuo

発現 

P 4H⁺

NAD⁺ NADH

乳酸 

ピルビン酸  アセチルCoA  TCA回路  酢酸 

電極 

dh ^QH2

Q

CymA e

-e

-ArcS

ArcA

nuo

抑制 

P 乳酸 

ピルビン酸 

アセチルCoA  酢酸  CO₂, 2H⁺

MtrB OmcA/

MtrC MtrA

MtrB OmcA/

MtrC MtrA

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Appendix

Appendix 1

Strain or plasmid Relevant characteristic Source of reference Escherichia coli

DH5α F-, φ 80dlacZ∆M15, ∆(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(r_K^-, m_K⁺), phoA, supE44, λ^-, thi^-1,

gyrA96, relA1

Takara

JM109 recA1. endAl, gyrA96, thi. hsdR17, supE44, relA1, λ^-,

∆(lac-proAB), [F’, traD36, proAB, lacI^q Z∆M15]

Takara JM109λpir JM109 lysogenized with λpir Penfold and

Pemberton, 1992 WM6026 lacI^q, rrnB3, DElacZ4787, hsdR514, DE(araBAD)567,

E(rhaBAD)568, rph-1,

att-lambda::pAE12-del(oriR6K-cat::frt5), DE(endA)::frt, uidA(delMluI)::pir(wt), attHK::pJK1006-del1/2

(deloriR6K-cat::frt5, deltrfA::frt)

William Metcalf, University of Illinois

BL21 DE3 F^– ompT hsdR17 (r_B^– m_B⁺) gal dcm(DE3) F^–, ompT, hsdSB (r_B^– m_B^–), gal(λcI 857, ind1, sam7, nin5,

lacUV5-T7gene1), dcm(DE3)

Novagen

S. oneidensis strain

MR-1 Wild type ATCC

ΔPFL SO_2912 pflB disruption This study ΔPDH SO_0424 aceE disruption This study ΔNDH SO_1017 nuoF , SO_3517 ndh , SO_0907 nqrF-1 ,

SO_1108 nqrF-2 disruption

This study ΔarcS SO_0577 arcS disruption This study ΔarcA SO_3988 arcA disruption This study ΔhyaB SO_2098 hyaB disruption This study ΔhydA SO_3920 hydA disruption This study ΔhydAΔhyaB SO_2098 hyaB , SO_3920 hydA disruption This study Δatp SO_4746 atpC to SO_4754 atpI disruption This study ΔubiA SO_0468 (ubiA) disruption This study ΔmenA SO_1910 (menA) disruption This study

ΔnuoF SO_1017 nuoF This study

Δndh SO_3517 ndh This study

ΔnqrF-1 SO_0907 nqrF-1 This study

ΔnqrF-2 SO_1108 nqrF-2 This study

ΔnuoFΔndh SO_1017 nuoF , SO_3517 ndh disruption This study ΔnuoFΔNDHΔubiA SO_1017 (nuoF), SO_3517 (ndh), SO_0468 (ubiA)

disruption

This study ΔnuoFΔNDHΔmenA SO_1017 (nuoF), SO_3517 (ndh), SO_1910 menA

disruption

This study NUO SO_3517 ndh , SO_0907 nqrF-1 , SO_1108 nqrF-2

disruption

This study NDH SO_1017 nuoF , SO_0907 nqrF-1 , SO_1108

nqrF-2 disruption

This study NQR1 SO_1017 nuoF , SO_3517 ndh , SO_1108 nqrF-2

disruption

This study NQR2 SO_1017 nuoF , SO_3517 ndh , SO_0907 nqrF-1

disruption

This study ΔcymA SO_4591 (cymA) disruption Bretschger et al.

2007

Appendix 1

Strain or plasmid Relevant characteristic Source of reference (omcA(mtrC SO_1779 (omcA), SO_1778 (mtrC) disruptionn Bretschger et al.

2007 ΔetrA SO_2356 (etrA) disruption This study Plasmid

pET-28 a Expression vector, T7 promoter Novagen

pET-arcA pET-28(a) containing N terminal His-tag-arcA This study pSMV-10 9.1 kb mobilizable suicide vector; oriR6K, mobRP4,

sacB, Km^r, Gm^r

Chad Saltikov, California Inst. of

Tech.

pSMV-pfl 1.6 kb fusion PCR fragment containing ∆pfl cloned into the SpeI site of pSMV-10

This study pSMV-aceE 1.6 kb fusion PCR fragment containing ∆aceE cloned

into the SpeI site of pSMV-10

This study pSMV-nuoF 1.6 kb fusion PCR fragment containing ∆nuoF cloned

into the SpeI site of pSMV-10

This study pSMV-ndh 1.6 kb fusion PCR fragment containing ∆ndh cloned into

the SpeI site of pSMV-10

This study pSMV-nqrF-1 1.6 kb fusion PCR fragment containing ∆nqrF-1 cloned

into the SpeI site of pSMV-10

This study pSMV-nqrF-2 1.6 kb fusion PCR fragment containing ∆nqrF-2 cloned

into the SpeI site of pSMV-10

This study pSMV-arcS 1.6 kb fusion PCR fragment containing ∆arcS cloned

into the SpeI site of pSMV-10

This study pSMV-arcA 1.6 kb fusion PCR fragment containing ∆arcA cloned

into the SpeI site of pSMV-10

This study pSMV-atp 1.6 kb fusion PCR fragment containing ∆atp cloned into

the SpeI site of pSMV-10

This study pSMV-hyaB 1.6 kb fusion PCR fragment containing ∆hyaB cloned

into the SpeI site of pSMV-10

This study pSMV-hydA 1.6 kb fusion PCR fragment containing ∆hydA cloned

into the SpeI site of pSMV-10

This study pSMV-ubiA 1.6 kb fusion PCR fragment containing ∆ubiA cloned

into the SpeI site of pSMV-10

This study pSMV-menA 1.6 kb fusion PCR fragment containing ∆menA cloned

into the SpeI site of pSMV-10

This study pSMV-etrA 1.6 kb fusion PCR fragment containing ∆etrA cloned into

the SpeI site of pSMV-10

This study pMElacZ pME4510 derivative, lacZ Gmr Endoh et al. 2003 pME-P_nuoA pMElacZ containing nuoA upstream region This study pME-P_{nuoA_TSS-7} pMElacZ containing region from -7 to +163 relative to

TSS_nuoA

This study pME-PnuoA_TSS-63 pMElacZ containing region from -63 to +163 relative to

TSSnuoA

This study pME-PnuoA_TSS-112 pMElacZ containing region from -112 to +163 relative

to TSSnuoA

This study pME-PnuoA_TSS-273 pMElacZ containing region from -273 to +163 relative

to TSSnuoA

This study pME-PnuoA_TSS-361 pMElacZ containing region from -361 to +163 relative

to TSSnuoA

This study pME-PnuoA_TSS-407 pMElacZ containing region from -407 to +163relative

to TSS_nuoA

This study pME-P_ndh pMElacZ containing ndh upstream region This study pME-Pnqr1 pMElacZ containing nqr1 upstream region This study pME-P_nqr2 pMElacZ containing nqr2 upstream region This study

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