xo℃εり；﹀り︒評住
200
100
o E EC
︾㊦で︒ り3＞りoO6住
300
200
100
Ortt 3 4
Days after injection
Fig．13 Effect of stimulants on erythrophagocytosis of elicited PM in vivo． PX obtained from mice injected i．p． with O．lml of BL， lml ef TG．
and 10e．ug of LPS， respectively， ldv4 days before harvest． Th6 resutt一一 ing macrophages were then lncubated with EC， phagocytosis assays as described in Materials and Metheds． Each values represents the mean
c
40
×③℃ε o聯＞ooσ6巳
20
OL 蝿鼈鼈黷st一一一一g一一一一Z3 4
Days after injection
Fig．14 Effect of stimulants on erythrophagocytosis of elicited PM in vivo． PM obtained from mice injected i．p． with O．lml of BL， 1．eml of TG， and 100，tzg of LPS， respectiveiy， 1 v4 days before harvest． The re−
sulting macrophages r−ere then ir）cubated with E， phagocytosis assays as described in Material＄ and Methods． Each values represents the meaB
±＄．E． of nine samples． A， BL； B， LPS； C， Tg
A
B
c
a
∵一巳⁝．チ﹁謬ドー．︾．・◎油壷
b
c
Fig．15 Phase contrast micrographs of elicited・一macrophages by stimuia一一 nts in vivo． The PN ebtained from injected i．p． with O．lml of BL，lml of TG，and 100，ug ef LPS，respectively，three days before harvested．
Total magnification is ×700． Panel a，cells are elicited by BL；b，cells are elicited by LPS；c，cells are elicited by TG．
xo℃二 り謂﹀りoO虐住
300
200
100
O U一一一一丁／ 一pt一一一M2 3
Days in cutture
Fig．16 Effect of cultivation on erythrophagocytosis of elicited PM．
The e1icited PM were harvested by the same method as those described in Fig．12． The resulting macrophages were cultured with RPMI−1640 me−
dium for the indicated periods． The capacity to injest EC was Lhen me−
asured， and then phagscytosis assays were carried out as described in Materials and Mehteds． Each values represents the meari ±S．E． of nirie
AB
c
D
×oηεり聯﹀り006匹
300
200
100
o
A
B
CD
o 1 2
Days in cutture
3
Fig．17 Effect of cultivation on erythrophagocytosis of BL−elicited PM．
BL−elicited PM were elicited the same method as those described in Fig．2． The resulting macrophages were cultured with RPM1一1640 medium for O−N−3 days leng， incubated with E or EC， and then phagocytosis ass一一 ays were carried uut as described in Materials and Mehtods．
Each values represent＄ the mean ±S．E． of nine samp1es．
200
xo℃εり；＞ooO毎氏
100
o o 30 60
1ncubation
90 120
time （min．）
150
Fig．18 Effect of incubation time of PM（lday cultured） with EC． The PM were incubated with RPMI−1640 medium containing 100ng／ml of PDA，
the fgllowed experimental conditions scere same as those described in Fig．13． Each values represents the mean ±S．E． of nine samples．
︾㊦で二 り欄ヴ﹀り00⑩巳
200
100
O 一ri一一 一一一 一一一wrm 一rrA一一T一．一A
3x 1び
O 3 10 3mo 10f
Concentration （ng l mt）
Fig．19 Effect of the concentration of PDA on erythrophagocytosis of PM（lday cultured）． The PM were incubated with RPMI−1640 medium contai一一 ning varieus concentration of PDA for 1 hr． Macrophage s monolayers were washed， covered with fresh RPXI−1640 medium， and added EC suspen−
sion， the follewing assays as described in Materials and Methods． Each values represents the mean ±S．E． ef nine samples．
×o℃εo；＞ooO虐巳
250
200
100
o
30 60 90 120 150
1ncubation time 〈min．）
Fig．20 Effect of two different phorbolesters on erythrophagocytosis of PM（lday cultured）． The PM（lday cultlired） uere incubated with RPMI−
1640 殖ediu顕 containing 10◎ng／田1 0f PDA or 100ngノ鵬l of P麗A for the ind−
icated periods， and then incubated with EC， phagocytosis assays were
×③℃ε り3＞り00価色
300
200
100
o
C．D
1
2Days
3
ject
4
Davs after iniection
Fig．21 Effect of PDA on erythrophagocytosis o．f BL−elicited PM． PM obt−
ained froift mice injected i．p． Nith O．im1 of BL 1−v4 days before harve−
st． The resulting macrophages were incubated with RPMI−1640 medium in presence or absence of 100ng／ml of PDA for 1 hr． The macrephage s mo−
nolayers were washed， cevered with the fresh RPK！一1640 medium， and ad−
ded E of EC suspension， the fo11owing assays as described in Materia1s and Methods． Each values represents the mean ±S．E． cf nine samples．
xoで⊆旧り3＞oqu而ユ
60
40
20
o
A
B
ハしD
1 2 3 4
Days after injection
Fig．22 Effect of PDA on erythrophaggcytosis of rG−elicited PM． PM ob−
tained from mice injected i．p． with 1．Oml of TG 1 v4 days before har−
vest． The resulting macrophages were incubated with RPMI−1640 medium in presence or absence of 100ng／ml of PDA fer 1 hr． The macrophage s monolayers were washed， cevered with the fresh RPMI−1640 medium， and added E or EC suspension， the following assays as described in Xateri−
als and Methods． Each values represents the mean ±S．E． of nine sampl−
×o℃三り3＞りoO6住
300
200
100
o
A
B
CD
1 2 3 4
Days after injection
Fig．23 Effect of PDA on erythrephagocytosis of LPS−elicited PK． P｝S ob−
tained from mice injected i．p． with 100，ttg of LPS 1・y4 days before ha−
rvest． The resulting macrophages were incubated with RPIil−1640 medium in presence or absence of leOng／ml of PDA for 1 hr． The macrophage s monolayers were washed， covered with the fresh RPMi一一1640 rr｝edium， and added E or EC suspension， the following assays as described in Materi−
als and Methods． Each values represents the mean ±S．E． of nine samp1es．
xoで︒欄り覆﹀りoO盤ユ
200
100
o A B c D E F
Fig．24 Effect ef PDA on erythrophagocytosis of PM elicited by stimul−
ants． The elicited PM were harvested as described in Fig．12． The resu−
lting macrophages were incubated with RPHI一！640 medium in presence er absence of leOng／ml of PDA for 1 hr． The macrophage s mgnelayers were washed， covered with fresh RPMI−1640 medium， and added EC suspension，
the foliowing assays as described in Materials and Methods． Each valu−
es represents the mean ±S．E． of nine samp1es． A，BL−elicited PM； B，
BL−elicited PM with PDA； C， Tg一一elicited PM； D， TG−e1icited PM with PBA
xo℃εo旧ヴ＞ooσσ匹
200
150
100
5G
O−T一一一一一丁2 一一一一一3
Days in cutture
Fig．25 Effect of PDA on erythrophagocytosis ef resident PM． Resident PM were harvested as described in Materials and Methods． The resulting macrophages were cultured with RPMI−164e medium in presence or absence ef 100／o of BL for 1 to 3 days， and then the macrophage s monolayers were washed， added the fresh RPMI−1640 medium in presence or absence
1OOng／ml of PDA， following binding and phagocytosis assays as describ−
ed in Materials and Methods． Each values represents the mean ±S．E．
of nine samples．