VP7㑇ఏᏊࡢ㛗ゎᯒࡢ⤖ᯝ㸪2002ᖺ࠾ࡼࡧ2005ᖺࡢྛᖺ࠾ࡅࡿྠ୍㎰ሙ
⏤᮶ᰴࡢ㑇ఏᏊሷᇶ㓄ิࡢ┦ྠᛶࡣ100㸣࡛࠶ࡗࡓࠋࡼࡗ࡚㸪ࡑࢀࡒࢀࡢⓎ⏕࡛
1㎰ሙࡘࡁ 1ᰴ㸪ྜィ 9ᰴࡢሷᇶ㓄ิࢆỴᐃࡋ㸪ࡑࢀࡒࢀࡢᰴD-2002㸪 E-2002㸪J-2002㸪L-2002㸪D-2005㸪E-2005㸪G-2005㸪K-2005࠾ࡼࡧL-2005
ྡࡅࡓࠋࡇࢀࡽࡢᰴࡢVP7㑇ఏᏊ㛗㸦UTRྵࡴ㸧ࡣ816bp࡛㸪ゎᯒ⏝
࠸ࡓᰴࡢ୰࡛ࡣ㸪1997ᖺ᳨ฟࡉࢀࡓBRVB Nemuroᰴ㸦AB016818㸧᭱ࡶ㏆
⦕࡛࠶ࡗࡓࠋ2002ᖺⓎ⏕ࡢᰴࡣ࡚ྠ୍࡛࠶ࡾ㸪 2005ᖺⓎ⏕ࡢᰴࡶ࡚
ྠ୍࡛࠶ࡗࡓࠋ2002ᖺ2005ᖺࡢᰴࢆẚ㍑ࡍࡿ5㹼6ሷᇶࡢ㐪࠸ࡀ࠶ࡾ㸪┦ྠ
ᛶࡣ99.3~99.4㸣࡛࠶ࡗࡓࠋࡉࡽ⣔⤫ᶞ࡛ࡣ2002ᖺ2005ᖺࡢᰴࡣ㸪␗࡞ࡿ
ࢡࣛࢫࢱ࣮ࢆᙧᡂࡋࡓ(ᅗ2-3a)ࠋ
VP4㑇ఏᏊࡢ5'ᮎ➃ࡢ1295 bpࡽ࡞ࡿሷᇶ㓄ิࡣ㸪 D-2002㸪E-2002㸪J-2002㸪 L2002㸪G-2005㸪K-2005࠾ࡼࡧL-2005ࡢ7ᰴࡘ࠸࡚ゎᯒࡋࡓࠋࡇࢀࡽࢆࢹ࣮
ࢱ࣮࣋ࢫୖࡢ∵࠾ࡼࡧࣄࢺࡢࡑࢀẚ㍑ࡋࡓࡇࢁ㸪ࣄࢺ⏤᮶ࡢRVBࡼࡾࡶ
ࣥࢻࡢ∵ࡼࡾ᳨ฟࡉࢀࡓBRVBᰴ ࡛ ࠶ ࡿRUBV226
ᰴ 㸪
RUBV282ᰴ ࠾ࡼࡧ DB176ᰴ㏆⦕࡛࠶ࡿࡀ㸪┦ྠᛶࡣ79㹼80㸣ẚ㍑ⓗప࠸ࡇࡀ☜ㄆࡉࢀࡓࠋ VP4㑇ఏᏊࡶVP7㑇ఏᏊྠᵝ㸪2002ᖺ2005ᖺࡢᰴࡣ⣔⤫ᶞ࡛␗࡞ࡿࢡࣛࢫࢱ28
࣮ᒓࡋࡓ㸦ᅗ2-3b)ࠋ
5. ⾑Ύᢠయ᳨ᰝ
BCoV㸪∵ࢹࣀ࢘ࣝࢫ7ᆺ࠾ࡼࡧBVDV-1ᆺ 2ᆺᑐࡍࡿᢠయ౯ࡣ㸪ྛᖺ ࡢࣉࣞ࠾ࡼࡧ࣏ࢫࢺ⾑Ύࡢẚ㍑࠾࠸࡚᭷ព࡞ୖ᪼ࢆㄆࡵ࡞ࡗࡓࠋBRVB࠾
࠸࡚ࡣ㸪2002ᖺࡣ20㢌ࡢ࠺ࡕ16㢌㸦80㸣㸧࠾ࡼࡧ2005ᖺࡣ28㢌ࡢ࠺ࡕ24㢌 㸦86㸣㸧࡛᭷ព࡞ᢠయୖ᪼㸦OD್ 0.2௨ୖ㸧ࢆㄆࡵࡓ㸦ᅗ2-4a࠾ࡼࡧb㸧ࠋࡇ
ࢀࡽࡢ⤖ᯝࡽ㸪2002ᖺ࠾ࡼࡧ2005ᖺࡢὶ⾜ᛶୗ⑩ࡣBRVB༢⊂ࡼࡿࡶࡢ
⪃࠼ࡽࢀࡓࠋࡲࡓ㸪Ⓨ⏕㎰ሙࡢᮍⓎ∵18㢌ࡢ࠺ࡕ13㢌ࡢ࣌⾑Ύ࡛BRVB ᑐࡍࡿⴭࡋ࠸ᢠయୖ᪼ࡀㄆࡵࡽࢀࡓ㸦ᅗ2-4c㸧ࠋ
2002ᖺࡢୗ⑩Ⓨ⏕4᭶๓ࡢⓎ⏕㎰ሙD࠾ࡼࡧE㎰ሙࡢBRVBᢠయ㝧ᛶ⋡ࡣࡑ
ࢀࡒࢀ32㸣࠾ࡼࡧ30㸣࡛࠶ࡗࡓࠋࡑࢀᑐࡋBRVBࡼࡿୗ⑩Ⓨ⏕ࡀ☜ㄆࡉࢀ
࡞ࡗࡓA㎰ሙ࠾ࡼࡧO㎰ሙࡢᢠయ㝧ᛶ⋡ࡣࡑࢀࡒࢀ67㸣࠾ࡼࡧ61㸣࡛࠶ࡾ㸪Ⓨ
⏕㎰ሙࡢᢠయ㝧ᛶ⋡ẚ㍑ࡋ࡚᭷ព㧗ࡗࡓ㸦p<0.01㸪⾲2-2㸧ࠋ
ࡲࡓ㸪2002ᖺⓎ⏕ࡢⓎ⏕㎰ሙD㸪E㸪J࠾ࡼࡧLࡢᛴᛶᮇࡢᢠయ㝧ᛶ⋡ࡣ15㸣
࡛࠶ࡗࡓ㸦ᅗ2-4a㸧ࠋ2005ᖺⓎ⏕ࡢ㎰ሙD㸪E㸪G㸪L࠾ࡼࡧQ㎰ሙࡢᛴᛶᮇ ࡢᢠయ㝧ᛶ⋡ࡣ26㸣࡛࠶ࡾ㸪㎰ሙD࠾ࡼࡧEࡢ㠀Ⓨ∵ࡢ67㸣ẚ㍑ࡋⴭࡋࡃప
ࡗࡓ(p<0.01㸪ᅗ2-4b࠾ࡼࡧc)ࠋ
2002ᖺⓎࡋࡓ7㢌୰4㢌㸦57㸣㸧ࡀ2005ᖺࡢⓎ⏕ࡶBRVB㑇ఏᏊࡀ㝧 ᛶ࡞ࡗࡓࠋࡉࡽࡇࢀࡽࡢ∵7㢌୰5㢌㸦71㸣㸧ࡣ2005ᖺࡢ࣌⾑Ύ࡛᫂ࡽ
࡞ᢠయୖ᪼ࡀㄆࡵࡽࢀࡓ㸦ᅗ2-5㸧ࠋࡋࡋࡇࢀࡽࡢ∵ࡣ2005ᖺୗ⑩≧ࢆ♧
ࡉ࡞ࡗࡓࠋ
29 6. ⣽⳦᳨ᰝ࠾ࡼࡧᐤ⏕᳨ᰝ
ࢧࣝࣔࢿࣛ⳦࣭ཎᛶ⭠⳦ࡢศ㞳ᇵ㣴᳨ᰝ,ࢥࢡࢩࢪ࣒࣭࢘ࢡࣜࣉࢺࢫ࣏ࣜ
ࢪ᳨࣒࢘ᰝࡣ࠸ࡎࢀࡶ㝜ᛶ࡛࠶ࡗࡓࠋ
30
⪃ ᐹ
2002ᖺBRVBࡼࡿୗ⑩ࡢὶ⾜ࡀⓎ⏕ࡋࡓ㎰ሙ࠾࠸࡚㸪ୗ⑩ὶ⾜๓ࡢ∵
⩌ࡢᢠయ㝧ᛶ⋡ࡣ㠀Ⓨ⏕㎰ሙࡢ∵⩌ࡼࡾࡶ᭷ពపࡗࡓ㸦⾲2-2㸧ࠋࡇࡢࡇ
ࡼࡾ㸪BRVBࡼࡿᡂ∵ࡢὶ⾜ᛶୗ⑩ࡢⓎ⏕ࡣ㸪ྠ࢘ࣝࢫᑐࡍࡿ⩌චࡢ పୗࡀ㛵ࡍࡿࡇࡀ♧၀ࡉࢀࡓࠋࣄࢺࡢRVAឤᰁ࡛ࡣ㸪⾑Ύ୰ࡢRVAᑐࡍ
ࡿᢠయ౯ࡣ㸪Ⓨ㜵ᚚࡢᣦᶆ࡞ࡿሗ࿌ࡉࢀ࡚࠸ࡿ㸦42,70,95㸧ࠋBRVBᑐ ࡍࡿᢠయࡶྠᵝⓎண㜵ຠᯝࢆᣢࡘணࡉࢀࡿࡇࡽ㸪㠀Ⓨ⏕㎰ሙࡢ∵
⩌࡛ࡣ㸪Ⓨ㜵ᚚ༑ศ࡞ᢠయࢆᣢࡘಶయࡀከᩘᏑᅾࡋࡓࡓࡵ㸪BRVBࡼࡿὶ
⾜ᛶୗ⑩ࡢⓎ⏕ࡀ㜼Ṇࡉࢀࡓ⪃࠼ࡽࢀࡓࠋ⯆῝࠸ࡇ㸪ᗂඣࡢࣄࢺRVA
࣡ࢡࢳࣥࡢ㞟ᅋ᥋✀ࡣ㸪࣡ࢡࢳࣥ᥋✀ࢆཷࡅ࡚࠸࡞࠸ᗂඣࡢⓎࢆண㜵ࡍࡿ
ࡉࢀ࡚࠸ࡿ㸦53㸧ࠋ௨ୖࡼࡾ㸪㠀Ⓨ⏕㎰ሙࡢ∵࠾ࡅࡿ⩌චࡀࠊBRVBᑐ ࡍࡿᢠయࢆಖ᭷ࡋ࡞࠸ಶయࡢⓎࢆண㜵ࡋࡓྍ⬟ᛶࡶ⪃࠼ࡽࢀࡓ(24,88)ࠋ
2005ᖺⓎ⏕ࡋࡓ2ᅇ┠ࡢὶ⾜ࡣ2002ᖺࡢὶ⾜ࢆ⤒㦂ࡋࡓࢇࡢ㎰ሙ࡛
Ⓨ⏕ࡋࡓࠋ㓗㎰ᅋᆅࡢྛ㎰ሙ࡛ࡣᦢங∵ࡢ⣙30㹼40㸣ࡀẖᖺ᭦᪂ࡉࢀ㸪᭦᪂ࡉ
ࢀࡓ㞤∵ࡢ⣙2/3ࡣ┴ෆእࡢ㎰ሙࡼࡾᑟධࡉࢀ࡚࠸ࡓࠋࡇࢀࡣ2ᅇ┠ࡢⓎ⏕๓ ࡢ2004ᖺ㡭ࡲ࡛㸪㎰ሙ࡛ᦢங∵ࡢ⣙༙ศ㏆ࡃࡀ2002ᖺࡢὶ⾜ࢆ⤒㦂ࡋ࡚࠸
࡞࠸ಶయ᭦᪂ࡉࢀ࡚࠸ࡓࡇࢆ♧ࡋ࡚࠸ࡿࠋࡋࡓࡀࡗ࡚㸪ᦢங∵ࡢ᭦᪂క
࠺⩌චࡢపୗࡀ2ᅇ┠ࡢୗ⑩ࡢⓎ⏕ࡘ࡞ࡀࡗࡓ⪃࠼ࡽࢀࡓࠋ
ࡲࡓ㸪2002ᖺ࠾ࡼࡧ2005ᖺὶ⾜ࡋࡓBRVBࡢVP7࠾ࡼࡧVP4㑇ఏᏊゎᯒࡢ
⤖ᯝ㸪ྛᖺࡢὶ⾜ᰴࡣ⣔⤫ᶞ࡛ࡣ␗࡞ࡿࢡࣛࢫࢱ࣮ࢆᙧᡂࡋࡓࡶࡢࡢ㸪ሷᇶ㓄
ิࡢ┦ྠᛶゎᯒ࡛ࡣ99.3㹼99.4㸣㧗࠸┦ྠᛶࢆ♧ࡋࡓࡇࡽ㸪2002ᖺ࠾
ࡅࡿὶ⾜ᰴࡀఱࡽࡢᙧ࡛㎰ሙෆᐃ╔ࡋ㸪2005ᖺ࠾ࡅࡿᗘ┠ࡢὶ⾜㛵
ࡋࡓྍ⬟ᛶࡀ㧗࠸⪃࠼ࡽࢀࡓࠋ
31
ࣄࢺ࡛ࡣ㏻ᖖ㸪RVAࡢึឤᰁ࡛ࡣ⤊⏕චࡀᡂ❧ࡏࡎ㸪ࡑࡢᚋࡶឤᰁࡍࡿ
ࡀ㸪≧ࡣ୍⯡ⓗ㔜⠜࡞ࡽ࡞࠸ሗ࿌ࡉࢀ࡚࠸ࡿ(21,25,56,94)ࠋࡉࡽ㸪
ࣄࢺRVAឤᰁ㛵ࡍࡿࢥ࣮࣍ࢺ◊✲࡛ࡣ㸪ྠࡌᆺࡢ࢘ࣝࢫࡢឤᰁᑐࡍࡿ
㜵ᚚචࡀ2ᖺ⥆ࡃࡇࡀ♧ࡉࢀࡓ㸦8㸧ࠋ୍᪉㸪RVAࡣᑐ↷ⓗRVBឤᰁࡢ ච㛵ࡍࡿሗࡣࢇ࡞࠸ࠋᅇ㸪VP7ࡸVP4㑇ఏᏊ㓄ิࡀᴟࡵ࡚㏆⦕࡞
BRVBࡀ⣙2ᖺ༙ࡢ㛫㝸࡛ᡂ∵ឤᰁࡋࡓࡇࡀ᫂ࡽ࡞ࡗࡓࠋࡋࡋ BRVBࡀ᳨ฟࡉࢀࡓࡶࢃࡽࡎ㸪ࡇࢀࡽࡢ∵ࡣ2ᅇ┠ࡢឤᰁୗ⑩≧ࢆ
♧ࡉ࡞ࡗࡓࠋࡇࢀࡽࡢ⤖ᯝࡽ㸪BRVBࡣᡂ∵ឤᰁࡍࡿࡀ㸪ឤᰁࡣ ࡑࢀ㔜⠜࡞≧ࢆࡶࡓࡽࡉ࡞࠸ࡇࡀ⪃࠼ࡽࢀࡓࠋࡇࡢࡇࡣࣄࢺࡢRVA ឤᰁ㠀ᖖ㢮ఝࡋࡓ⌧㇟࡛࠶ࡾ㸪BRVBࡼࡿୗ⑩ண㜵⟇ࢆㅮࡌࡿୖ࡛ࡢཧ⪃
࡞ࡿᛮࢃࢀࡓࠋ
ᅇ㸪BRVBࡣྠ୍㎰ሙෆ࡛ࡶᩘᖺ㛫㝸࡛ᡂ∵ࡢἪங㔞ࡢῶᑡὶ⾜ᛶୗ⑩ࢆ
ᘬࡁ㉳ࡇࡋࡓࠋࡑࡢせᅉࡋ࡚㸪⩌චࡀపୗࡋ࡚࠸ࡓྍ⬟ᛶࡀ⪃࠼ࡽࢀࡓࠋ ࡉࡽྠ୍∵࡛ࡶRVBឤᰁࢆ⧞ࡾ㏉ࡍࡇࡀุ᫂ࡋࡓࠋᚋ㸪BRVB᳨ฟࡢ⡆
᫆ⓗ᳨ᰝἲࡀ㛤Ⓨࡉࢀ㸪BRVBࡢࡉࡽ࡞ࡿᏛᐇែࡀゎ᫂ࡉࢀࡿࡇࢆᮇᚅࡋࡓ
࠸ࠋ
32
ᅗ ⾲
⾲2-1. ゎᯒࡋࡓBRVBࡢDDBJ/EMBL/GenBankࢡࢭࢵࢩࣙࣥ␒ྕ
Accession No. Strain Region
LC005523 D-2002 VP7
LC005524 E-2002 VP7
LC005525 J-2002 VP7
LC005526 L-2002 VP7
LC005527 D-2005 VP7
LC005528 E-2005 VP7
LC005529 G-2005 VP7
LC005530 K-2005 VP7
LC005531 L-2005 VP7
LC005532 D-2002 VP4
LC005533 E-2002 VP4
LC005534 J-2002 VP4
LC005535 L-2002 VP4
LC005536 G-2005 VP4
LC005537 K-2005 VP4
LC005538 L-2005 VP4
33
ᅗ2-1. 2002ᖺ࠾ࡼࡧ2005ᖺBRVBࡀὶ⾜ࡋࡓ㓗㎰ᅋᆅࡢᴫ␎ᅗ
ࣝࣇ࣋ࢵࢺࡣ㎰ሙྡࢆ♧ࡍࠋ
ⰍࡁࡣBRVBࡼࡿୗ⑩Ⓨ⏕㎰ᐙࢆ♧ࡍࠋ
✵ⓑࡣ✵ࡁ␆⯋ࢆ♧ࡍࠋ
15 m 200 m 15 m
In 2002
In 2005
A A B B B B
I J K
y
empty empty empty
C D
L M E N
F G H O P Q
K
D L M
E N
F G H O P Q
Outbreak
farm Non-outbreak
farm
34
ᅗ2-2㸬2002ᖺ2005ᖺࡢBRVB RNA㟁ẼὋືࣃࢱ࣮ࣥ
࣮ࣞࣥࡢࣝࣇ࣋ࢵࢺࡣ㎰ሙྡࢆ♧ࡍࠋ
E࠾ࡼࡧDࡣ2002ᖺ㸪L㸪K㸪G࠾ࡼࡧQࡣ2005ᖺࡢ᳨ฟᰴࠋ RVAࡣRVAཧ↷ᰴ(OSUᰴ)ࠋ
ᩘᏐ▮༳ࡣࣟࢱ࢘ࣝࢫࡢ㑇ఏᏊศ⠇ࢆ♧ࡍࠋ
E D L K G Q
2002 2005
RVA
Fig. 2
1 2 34
56
78 910
11
1 23 4
5 6
789
10 11 10
11
1 2 3 4
9 10 11 9
7 8 5 6
35
⾲2-2. 2002ᖺࡢୗ⑩Ⓨ⏕4᭶๓ࡢᡂ∵ࡢBRVBᢠయ᳨ᰝᡂ⦼
㝧ᛶᩘ ᳨యᩘ ྜ(%)
Da 21 66 31.8A
Ea 21 69 30.4A
Ab 79 118 66.9B
Ob 43 71 60.6B
㎰ሙ RVBᢠయ᳨ฟ
a 4ࣨ᭶ᚋὶ⾜ᛶRVBࡼࡿୗ⑩Ⓨ⏕
b 4ࣨ᭶ᚋὶ⾜ᛶRVBࡼࡿୗ⑩Ⓨ⏕࡞ࡋ A,B ␗➢ྕ㛫࡛᭷ពᕪ࠶ࡾ (P<0.01)
36
ᅗ2-3. ∵࠾ࡼࡧࣄࢺ⏤᮶RVBࡢVP7㑇ఏᏊ㸦a㸧࠾ࡼࡧVP4㑇ఏᏊ5'ᮎ➃㡿 ᇦ㸦b㸧ࡢሷᇶ㓄ิᇶ࡙ࡃศᏊ⣔⤫ᶞ
ࣈ࣮ࢺࢫࢺࣛࢵࣉ್ࢆศᒱࡢ⫪♧ࡍࠋ
㻜 㻟㻠㻚㻠
㻡 㻝㻜
㻝㻡 㻞㻜
㻞㻡 㻟㻜
RVB/Cattle-wt/JPN/G-2005/2005/G3PX RVB/Cattle-wt/JPN/K-2005/2005/G3PX RVB/Cattle-wt/JPN/D-2005/2005/G3PX RVB/Cattle-wt/JPN/L-2005/2005/G3PX RVB/Cattle-wt/JPN/E-2005/2005/G3PX RVB/Cattle-wt/JPN/D-2002/2002/G3PX RVB/Cattle-wt/JPN/L-2002/2002/G3PX RVB/Cattle-wt/JPN/E-2002/2002/G3PX RVB/Cattle-wt/JPN/J-2002/2002/G3PX
㻥㻡
RVB/Cattle-wt/JPN/Nemuro/1997/G3PX
㻝㻜㻜
RVB/Cattle-wt/USA/MN10-1/2010/G3PX RVB/Cattle-wt/USA/ATI/19XX/G3PX
RVB/Cattle-wt/USA/WD653/19XX/G3PX RVB/Cattle-wt/USA/Mebus/19XX/G3PX RVB/Cattle-wt/IND/DB101/2001/G5PX
RVB/Cattle-wt/IND/DB180/2001/G5PX
㻤㻥
RVB/Cattle-wt/IND/DB176/2001/G5PX
㻝㻜㻜
㻝㻜㻜
RVB/Human-wt/IND/CAL/1998/G2PX RVB/Human-wt/BGD/Bang373/2000/G5PX
㻝㻜㻜
RVB/Human-wt/CHN/WH-1/2002/G5PX RVB/Human-wt/CHN/ADRV/1986/G5PX
㻝㻜㻜
㻝㻜㻜
㻥㻟
a
b
㻜 㻟㻡㻚㻣
㻡 㻝㻜
㻝㻡 㻞㻜
㻞㻡 㻟㻜
㻟㻡
RVB/Cattle-wt/JPN/D-2002/2002/G3PX RVB/Cattle-wt/JPN/L-2002/2002/G3PX RVB/Cattle-wt/JPN/E-2002/2002/G3PX RVB/Cattle-wt/JPN/J-2002/2002/G3PX RVB/Cattle-wt/JPN/K-2005/2005/G3PX RVB/Cattle-wt/JPN/L-2005/2005/G3PX RVB/Cattle-wt/JPN/G-2005/2005/G3PX RVB/Cattle-wt/IND/RUBV226/2004/G5PX RVB/Cattle-wt/IND/RUBV282/2005/G5PX RVB/Cattle-wt/IND/DB176/2001/G5PX RVB/Human-wt/IND/IC-008/2008/G2PX
RVB/Human-wt-/IND/NIV-094456/2009/G2PX RVB/Human-wt/IND/CAL/1998/G2PX RVB/Human-wt/BGD//Bang373/2000/G2PX RVB/Human-wt/CHN/ADRV/1986/G2PX RVB/Human-wt/CHN/WH-1/2002/G2PX
㻣㻣 㻣㻜 㻥㻣 㻤㻤
㻝㻜㻜 㻝㻜㻜 㻝㻜㻜
㻝㻜㻜 㻝㻜㻜 㻤㻢 㻝㻜㻜
㻝㻜㻜
Fig. 5
37
ᅗ2-4㸬2002ᖺ㸦a㸧࠾ࡼࡧ2005ᖺ㸦b࠾ࡼࡧc㸧ࡢୗ⑩ⓎࡶࡋࡃࡣᮍⓎ∵ࡢ
࣌⾑ΎࡢBRVBᢠయࡢ᥎⛣
Ⅼ⥺ࡣOD್0.2ࢆ♧ࡋࠊࡇࢀࡼࡾୖ࡛BRVBᢠయ㝧ᛶ࡛࠶ࡿุᐃࡍࡿࠋ
࣌⾑Ύ࡛OD್0.2௨ୖࡢᕪࡀぢࡽࢀࡓሙྜࢆ⾑Ύᛂࡀ࠶ࡗࡓぢ࡞ࡍࠋ 2002ᖺⓎ⏕ࡢⓎ⏕㎰ሙࡢᛴᛶᮇࡢᢠయ㝧ᛶ⋡ࡣ15㸣ࠋ
2005ᖺⓎ⏕ࡢⓎ⏕㎰ሙࡢᛴᛶᮇࡢୗ⑩Ⓨ∵ࡢᢠయ㝧ᛶ⋡ࡣ26㸣㸪 㠀Ⓨ∵ࡢ67㸣ẚ㍑ࡋⴭࡋࡃపࡗࡓ(p<0.01)ࠋ
38
ᅗ2-5. 2002ᖺ࠾ࡼࡧ2005ᖺࡢྠ୍∵㸦7㢌㸧࡛ࡢBRVBᢠయࡢ᥎⛣ୗ⑩ࡢ
≧ࡢ᭷↓࠾ࡼࡧBRVB≉␗㑇ఏᏊ᳨ฟࡢ㛵ಀ
Ⅼ⥺ࡣOD್0.2ࢆ♧ࡋࠊࡇࢀࡼࡾୖ࡛BRVBᢠయ㝧ᛶ࡛࠶ࡿุᐃࡍࡿࠋ
࣌⾑Ύ࡛OD್0.2௨ୖࡢᕪࡀぢࡽࢀࡓሙྜࢆ⾑Ύᛂࡀ࠶ࡗࡓぢ࡞ࡍࠋ
39
➨ ୕ ❶
▼ᕝ┴᳨࡛ฟࡉࢀࡓBRVBࡢ㑇ఏᏊゎᯒ
40 ᗎ ㄽ
ࣟࢱ࢘ࣝࢫࡣ11ᮏࡢ2ᮏ㙐RNAࢭࢢ࣓ࣥࢺࢆ㑇ఏᏊࡋ࡚ಖ᭷ࡍࡿࠋࡇ
ࢀࡲ࡛ࣟࢱ࢘ࣝࢫ࡛ࡣ㜵ᚚචࢆㄏᑟࡍࡿእẆ⺮ⓑ࡛࠶ࡿVP7࠾ࡼࡧࢫࣃ
ࢡ⺮ⓑ࡛࠶ࡿ VP4 ࡢ㑇ఏᏊ㓄ิࡼࡿ GX-P[X] 㸦X ࡣ㑇ఏᏊᆺ␒ྕࢆ♧ࡍ㸧 㑇ఏᏊᆺูࡀ⾜ࢃࢀ࡚ࡁࡓ㸦20㸧ࠋ୍᪉㸪」ᩘࡢࣟࢱ࢘ࣝࢫᰴࡀྠ୍ࡢ⣽⬊
ឤᰁࡋࡓሙྜ㸪㑇ఏᏊࢭࢢ࣓ࣥࢺࡢ㸦㑇ఏᏊ㞟ྜ㸧ࡀ㉳ࡇࡾ㸪⤖ᯝࡋ
࡚ぶᰴࡣ␗࡞ࡿࢭࢢ࣓ࣥࢺࡢ⤌ࡳྜࢃࡏࢆᣢࡘᏊᏞ࢘ࣝࢫᰴࡀㄌ⏕ࡍࡿࠋ
ࣟࢱ࢘ࣝࢫࡣᰴ㛫࡛㑇ఏᏊࢭࢢ࣓ࣥࢺࢆࡍࡿ㑇ఏᏊ㞟ྜࢆ㉳ࡇࡋ㸪ࡑ ࡢ⤖ᯝ」㞧࡞㑇ఏᏊᆺࢆᣢࡘᰴࡀㄌ⏕ࡍࡿࠋᚑ᮶ࡢGX-P[X]ᆺู࡛ࡣVP7࠾ࡼ
ࡧVP4㑇ఏᏊ௨እࡢࢭࢢ࣓ࣥࢺࡢሗࡀḞⴠࡍࡿࡓࡵ㸪ࡢࢭࢢ࣓ࣥࢺࡀ㛵
ࡍࡿ㑇ఏᏊ㞟ྜᰴࢆ༊ูࡍࡿࡇࡀ࡛ࡁ࡞࠸㸦30,32,59,68㸧ࠋࡇࢀࡽࡢࡇ
ࡽ㸪Rotavirus Classification Working Groupࡽࣟࢱ࢘ࣝࢫࡢࢭࢢ࣓ࣥࢺ ࡢ 㑇 ఏ Ꮚ 㓄 ิ ᇶ ࡙ ࡃ 㑇 ఏ Ꮚ ᆺ ู ࡀ ᥦ ၐ ࡉ ࢀ 㸪 ࢭ ࢢ ࣓ ࣥ ࢺ VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 ࡢ ྛ 㑇 ఏ Ꮚ ᆺ
ࢆ GX-P[X]-IX-RX-CX-MX-AX-NX-TX-EX-HX㸦X ࡣ㑇ఏᏊᆺ␒ྕࢆ♧ࡍ㸧
♧ࡍࡇ࡞ࡗࡓ㸦59,60㸧ࠋࡇࡢࢩࢫࢸ࣒ࡀ 2008 ᖺᥦၐࡉࢀ࡚௨᮶㸪RVA
࡛ࡣᩘከࡃࡢ㑇ఏᏊࢹ࣮ࢱࡀ DDBJ/EMBL/GenBank ࢹ࣮ࢱ࣮࣋ࢫⓏ㘓ࡉࢀ
ࡓࠋࡋࡋ㸪RVB ࡘ࠸࡚ࡣࣄࢺ㸪㇜࠾ࡼࡧࣛࢵࢺ⏤᮶ᰴࡢᩘᰴࡘ࠸࡚㑇 ఏᏊ㓄ิࡀⓏ㘓ࡉࢀ࡚࠸ࡿ㐣ࡂࡎ㸪BRVB㛵ࡋ࡚ࡣVP7㸪VP4㸪VP6࠾ࡼ
ࡧNSP5ࡢ㓄ิࡀᩘᰴ㸪VP1㸪VP2㸪NSP1-࣌ࣉࢳࢻ1࣭࣌ࣉࢳࢻ2࠾ࡼࡧNSP2 ࡢ㓄ิࡣ 2 ᰴࡋⓏ㘓ࡉࢀ࡚࠸࡞࠸㸦31,33,91,93㸧ࠋࡉࡽ VP3㸪NSP3 ࠾ࡼ
ࡧNSP4ࡢ㑇ఏᏊ㓄ิ㛵ࡋ࡚ࡢⓏ㘓ࡣⓙ↓࡛࠶ࡿࠋ
ࡑࡇ࡛▼ᕝ┴᳨࡛ฟࡉࢀࡓ BRVB ࡢࢤࣀ࣒ RNAࢆḟୡ௦ࢩ࣮ࢡ࢚ࣥࢧ࣮࡛
ゎᯒࡋ㸪ࡇࢀࡲ࡛᫂ࡽ࡞ࡗ࡚࠸࡞࠸VP3㸪NSP3࠾ࡼࡧ NSP4㑇ఏᏊ㓄ิ
41
ࢆỴᐃࡋࡓࠋࡲࡓ㸪ᚓࡽࢀࡓྛ㑇ఏᏊࡢሷᇶ㓄ิࢆศᏊ⣔⤫Ꮫⓗゎᯒࡸࡑࡢ
ࡢ㑇ఏᏊゎᯒ౪ࡋࡓୖ࡛ࠊྛ࢘ࣝࢫ⺮ⓑ㉁ࡢศᏊ⏕≀Ꮫⓗᛶ≧ࡘ࠸࡚⪃
ᐹࡋࡓࠋ
42
ᮦᩱ࠾ࡼࡧ᪉ἲ
1.㑇ఏᏊ㓄ิỴᐃࡢࡓࡵࡢ⣅౽ࢧࣥࣉࣝࡢ㑅ᐃ
➨୍❶࠾ࡼࡧ➨❶࡛⏝࠸ࡓ⣅౽ࡢ࠺ࡕ㸪BRVBᶆⓗ RT-PCR࡛㝧ᛶ࡞ࡗ ࡓ㸪ࡑࢀࡒࢀ1ᡞ1᳨య࠾ࡼࡧ3ᡞ5᳨యࢆ㑅ᐃࡋࡓࠋࡉࡽ㸪BRVBࡼࡿ
ᡂ∵ୗ⑩ࡢⓎ⏕ࡀ࠶ࡗࡓ㸪2002ᖺ▼ᕝ┴Hᕷࡢ1ᡞ3᳨య㸪࠾ࡼࡧ2006ᖺ
࠾ࡼࡧ2011ᖺ➨❶ྠࡌ㓗㎰ᅋᆅࡢ3ᡞ7᳨యࢆ㑅ࢇࡔࠋࡍ࡞ࢃࡕ㸪ྜィ 16 ᳨యࡢ⣅౽ஙࢆ⏝࠸㸪ḟୡ௦ࢩ࣮ࢡ࢚ࣥࢫࡢࡓࡵࡢࣛࣈ࣮ࣛࣜస〇ࢆ⾜
ࡗࡓࠋ