図 10 rp30 感作マウス血清の peptide 6 および 15 に対する 競合反応性比較
rp30 感作マウス血清と各濃度に調整した peptide を各々反応させ、
peptide 6 ならびに 15 に対する競合反応の有無を確認した。
Y 軸 : B / B0 = 各抗原濃度添加における OD 値 (=B)/ 抗原未添加に おける OD 値 (= B0)
0 0.2 0.4 0.6 0.8 1 1.2
1 10 100 1000 10000
peptide Z x (nM)
B /B 0
peptide 6
図 11 p30 ペプチド刺激に対する p30 感作マウス脾臓内 T 細胞の IFN-γ 産生量
rp30 感作マウス脾臓内 T 細胞に各 p30 ペプチド 5 μM を添加して 3 日間培養し、培養上清中の IFN-γ 量を ELISA 法にて測定した。
C は in vitro において rp30 を添加培養した群（陽性コントロール ) 。
＊ rp30 添加培養群 (C) に対して有意差あり (p < 0.05)
0 50 100 150 200
IFN-γ（pg/ml)
C 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
* * *
*
10 20 30 40 50 60 MSVSLHHFII SSGFLTSMFP KAVRRAVTAG VFAAPTLMSF LRCGVMASDP PLVANQVVTC * *** **** *** ** * 70 80 90 100 110 120 PDKKSTAAVI LTPTENHFTL KCPKTALTEP PTLAYSPNRQ ICPAGTTSSC TSKAVTLSSL
***** ***** **** * *** ***** ********* ***
130 140 150 160 170 180 IPEAEDSWWT GDSASLDTAG IKLTVPIEKF PVTTQTFVVG CIKGDDAQSC MVTVTVQARA ****** ********** ********
190 200 210 220 230 240 SSVVNNVARC SYGADSTLGP VKLSAEGPTT MTLVCGKDGV KVPQDNNQYC SGTTLTGCNE *** ********* ** ******* **** ******** *** *****
250 260 270 280 290 300 KSFKDILPKL TENPWQGNAS SDKGATLTIK KEAFPAESKS VIIGCTGGSP EKHHCTVKLE
* ******* ***** *** * ****** ***
310 320 330 340 FAGAAGSAKS AAG TASHVSI FAMVIGLIGS IAACVA
****
図12 p30アミノ酸配列の2次構造予測-Genetyxによる解析
N末端シグナル配列は太字で、細胞膜貫通領域は太下線で示した。
rp30 感作マウス血清のIgG2a反応領域はbox で、IFN- 産生への関与が
67
10 20 30 40 50 60 MSVSLHHFII SSGFLTSMFP KAVRRAVTAG VFAAPTLMSF LRCGVMASDP PLVANQVVTC
*****
70 80 90 100 110 120 PDKKSTAAVI LTPTENHFTL KCPKTALTEP PTLAYSPNRQ ICPAGTTSSC TSKAVTLSSL ********* ** **** * **** * ***
130 140 150 160 170 180 IPEAEDSWWT GDSASLDTAG IKLTVPIEKF PVTTQTFVVG CIKGDDAQSC MVTVTVQARA **** **** ****** **** * ******
190 200 210 220 230 240 SSVVNNVARC SYGADSTLGP VKLSAEGPTT MTLVCGKDGV KVPQDNNQYC SGTTLTGCNE *** *** ***** *** * **** ** ***** *** **** * 250 260 270 280 290 300 KSFKDILPKL TENPWQGNAS SDKGATLTIK KEAFPAESKS VIIGCTGGSP EKHHCTVKLE
** ****** ******** ** ******* ** **********
310 320 330 FAGAAGSAKS AAGTASHVSI FAMVIGLIGS IAACVA
*
図13 p30アミノ酸配列の2次構造予測-UniProtおよびSOSUIによる解析
N末端シグナル配列は太字で、膜貫通領域は太下線で示した。
rp30 感作マウス血清のIgG2a反応領域はbox で、IFN- 産生への関与が推測された
68
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