Studies on Micro Methods for the Determination of Sugars Part 1 On the Calculating Table of Xylose by Iodometry

奈良教育大学学術リポジトリNEAR

Studies on Micro Methods for the Determination of Sugars Part 1 On the Calculating Table of Xylose by Iodometry

著者 TAKAHASHI Mitsuo

journal or

publication title

奈良学芸大学紀要

volume 4

number 2

page range 91‑95

year 1954‑12‑25

URL http://hdl.handle.net/10105/5061

Studies on Micro Methods for the Determination of Sugars

Part 1 On the Calculating Table of

Xylose by lodometry Mitsuo TAKAHASIII Laboratory of Agricultural Chemistry

Nara Gakngei University (Received September 1, 1954J

(O.C2)

FJic author lias already reported a micro method for the determination of

xylose. The method was accomplished by the modification of iodometry.

For convenience* sake in calculation, the author has made the calculating table

80 that it may be possible to obtain milligrams of xylose directly from the

difference in titration between the blank and the sample. The author wants to

report the calculating table in this paper. Ent, only the method of analysis and the calculating equation are given here, since a detailed description has already

CD,(2)

been reported by the author.

Method of Analysis 1- Composition of micro reagent

gr. per litre

CuSO4. 5H2O 5.0

Tartaric acid 7.5

NaoCO.-; (anhydrous) 40.0

Kl 10.0

K1O« 0. 7

Potassium oxalato 18.4

2- Determination

Place 5 ce of the micro reagent in a 100 ec Erlenmeyer flask with plug and to

this add 5 cc of the sample. At the same time make up a blank with 5 ce of

water and 5 ec of reagent. Put a stopper on the flask with loose fitting plug and heat for 15 minutes in a boiling water bath. Cool in water, add?>.(> ce of IN

H2MO4, shake well and titrate with 0.01 N thiosulphate and starch paste as an

indicator.

The Calculating Equation

Milligrams of xylose in the calculating table were calculated by the next

equation.

Journal of Nara G-akiigei University Vol.4 No.2, PEC. ?, 20 19.54

(92) Mitsuo TAKAHASHI Y=0.10fi8n + 0.27O7X-0.O0181X"

X: the difference in titration of 0,01 JSr tbiosulphate between the blank and the sample

Y : milligrams of xylose

Table 1. The Calculating Table of Xylose

V i m N a 2 S .,O n

  c c

X y l o s e  m s r N / i o o

N a 2 S a O s  X y l o s e  n i g N / i o o N a o S 3 0 3

  c c

X y l o s em g  N a 3 S 2 O 8N / i o oc c

X y l o s e  1U LJ

1 .3 6 0 . 4 7 2 1 .7 6     0 .5 7 8 2 .1 fi    0 .6 8 3    i 2 . 5 6 0 .7 8 8 1 .3 7 0 . 4 7 4 0 .5 .S O 2 .1 7 O .fi 8 6     9 . 5 7 0 .7 9 1 i ..s s 0 .4 7 7 1 .7 S     O ‑ 3 S 3 2 .1 8 0 .6 8 8 2 .5 8 0 .7 9 3 1 .3 9 0 4 S O 1 .7 9     0 .5 8 6 2 .1 9 0 .6 9 1 2 .5 9 0 .7 9 6 1 .4 0 0 . 4 8 2 1 .8 0     0 .5 8 8 2 .2 0 0 .6 9 4     2 .  6 0 0 .7 9 8 1 .4 1 0 4 8 5 1 .8 1 O .? 9 1 2 .2 1 0 .6 9 P ) 2 .fi l 0 .8 0 1 1 .4 2 0 . 4 8 8 1 .8 2     0 .5 ! )4 9  *> 9 0 .6 9 9 2 .0 2 0 .8 0 4 1 .4 '} 0 . 4 0 0 1 . 8 .'!     o . r>si fi 2 .2 '! 0 .7 0 2     2 .6 3 0 .8 0 6 1 .4 4 0 .4 9 '! 1 .X 4 0 .  5 9 9 2 .2 4 0 .7 0 4     2 .6 4 0 .8 0 9 1 . 4 5 0 . 4 ! ｻH 1 .8 5 0 .0 0 1 i :i r> 0 .7 0 7 2 .6 5 0 .8 1 2

1 .4 6 0 . 4 9 8 1 .8 6 0 .B O 4 2 .2 6 0 .  7 0 9 2 .fi fi 0 .8 1 4 1 .4 7 0 . 5 0 1 1 .8 7 0 .6 0 7 2 .2 7 0 .7 1 2 2 .6 7 0 .8 1 7 1 . 4 8 0 .  5 0 .'‑} 1 .8 8 0 .6 1 0 2 .2 8 0 .7 1 5 2 .6 8 0 .8 1 9 1 . 4 9 0 .6 0 6 1 .8 9 O .fi 1 2 2 .2 9 0 .7 1 7 2 .fi 9 0 .8 2 2 1 .5 0 0 . 5 0 9 1 .9 0 0 .6 1 5 2 .3 0 0 .7 2 0 2 .7 0 ‑ 0 .8 2 5 1 . 5 1 0 . 5 1 2 1 . 9 1 0 . 6 1 8 2 .3 1 0 .7 2 3 2 .7 1 0 .8 2 7 1 .5 2 0 . 5 1 4 1 .9 2 O .fi 2 O 蝣?  'W , 0 .7 2 5 2 .7 2 0 .8 3 0 1 .5 3 0 . 5 1 7 1 .9 3 0 .0 2 .3 2 .3 3 0 .7 2 8 2 .7 ‑i 0 .8 3 2 1 .5 4 0 . 5 1 9 1 .9 4 0 .6 2 5 2 .3 4 0 .7 ‑S O 2 .7 4 0 .8 3 5 1 .5 5 0 . 5 2 2 1 .9 5 0 . 6 2 8 2 .3 5 0 .7 2 .7 5 0 .8 3 8

1 .5 8 0 .^ 2 5 1 .9 6 O .fi 'l O 2 .3 fi 0 .7 3 6 2 . 7 fi 0 .8 4 0 1 .5 7 0 . 5 2 7 1 .9 7 0 .6 3 3 2 .: )', 0 .7 3 8 2 .7 7 0 .8 4 .0 1 .5 8 0 .  5 .'!0 1 .9 8 0 .6 " 6 2 .3 8 0 .7 4 1 2 .7 S 0 .8 4 5 1 .5 9 0 .5 :j .'J 1 .9 9 0 .6 :5 8 2 .3 9 0 .7 4 3 2 . 7 9 0 .8 4 9 1 .6 0 0 .5 3 5 2 . 0 0 0 .6 4 1 2 .4 0 0 .  7 4 6 2 .8 0 0 .8 5 2 1 .6 1 0 .  5 3 $ 2 .0 1 0 .6 4 4 2 . 4 1 0 .7 4 9 2 .8 1 0 .8 5 4 I .  fi 2 0 .5 4 1 2 .0 2 0 .6 4 6 2 .4 2 0 .T 5 1 2 .8 2 0 .8 5 fi 1 .6 3 0 .5 4 3 2 .0 ,1 0 .6 4 9 2 .4 3 0 .7 5 4 2 .8 3 0 .8 5 8 1 .6 4 0 . 5 4 6 2 . 0 4 O .  fi 5 2 2 .4 4 0 .7 5 7 2 .8 4 0 .8 6 1 l .fi S 0 . 5 4 9 2 .0 5 O .fi 5 4 2 ‑4 5 0 .  7 5 9 2 .8 5 0 .8 fi 4 l . fi fi 0 . 5 5 1 2 .0 6 0 .6 5 7 2 .4 fi 0 .  7 6 2 2 .8 fi 0 .8 6 (5 1 .H 7 0 . 5 5 4 2 .0 7 0 . 8 5 9 2 .4 7 0 .7 6 4 2 .8 7 O .W J l .fi S 0 .5 5 7 2 .0 8 O .fi fi 2 2 .4 8 0 .7 0 7 2 .8 8 0 .8 7 1 1 .6 9 0 .5 .r)9 2 .0 9 O .fi 6 5 2 .4 9 0 .7 7 0 2 .8 9 0 .8 7 4 1 .7 0 0 .  5 6 2 2 . 1 0 0 .6 6 7 2 .5 0 0 .7 7 2 2 .9 0 0 .8 7 7 1 .7 1 0 .5 fi 4 2 .l l     O .fi T O 2 .5 1 0 .7 '      2 .9 1 0 .8 7 9 1 .7 2 0 .5 fi 7 2 . 1 2 0 . 6 7 3 2 .5 2 0 .7 7 8 2 .  9 2 0 .8 8 2 l .v ; 0 .5 7 0 2 1 3     0 6 7 5 2  ‑5 .3 0 .7 8 0     2 .9 .'J 0 .S S 4 1 .7 4 0 .5 7 2 2 . 1 4     0 .6 7 8 2 . 5 4 0 .7 8 3     2 .9 4 0 .8 8 7 1 . 7 5 0 .5 7 5 2 . 1 5     0 . 6 8 1  2 .  ">5

・ I

0 .7 8 5 2 .9 5 0 .8 9 0

N / i o o

N a 3 S 2 O 3  X y lo s e;ra g  ra g  N a ^N / i o oO oc c  c c  X y ln s em g  m g  N a o 8 3 O sN / i o oc c  c c X y l o s em g   N / i o o N a 3 S 2 O 8

  c c

X y lo s e  m g

4 .4 6 4 .4 7 4 .4 8 4 .4 9 4 .5 0 4 . 5 1 4 . 5 2 4 .5 :;

4 . 5 4 4 . 5 5 4 .5 6 4 .5 7 4 .5 8 4 . 5 9 4 .6 0 4 .6 1 4 .6 2 4 .6 5 4 . 6 4 4 .6 5 4 . 6 6 4 .6 7 4 . H 8 4 . 6 9 4 . 7 0 4 . 7 1 4 . 7 2 4 . 7 3 4 . 7 4 4 . 7 5 4 .7 6 4 .7 7 4 .7 8 4 .7 9 4 .8 0 4 .8 1 4 .8 2 4 .8 3 4 .8 4 4 .8 5 4 .8 6 4 .8 7 4 .8 8 4 .8 9

1 .2 7 8 1 .2 8 1 1 .2 8 3 1 .2 8 6 1 .2 8 9 1 .2 9 1 1 .2 3 3 1 .2 9 H i .2 y y 1 .3 0 1 1 .3 0 4 1 .3 0 6 1 .3 0 9 1 .3 1 1 1 .3 1 4 1 .3 1 6 1 .3 1 9 1 .3 2 1 1 .3 2 4 1 .3 2 6 1 .3 2 9 1 .8 3 2 1 .3 3 4 1 .3 3 7 1 .3 3 9 1 .3 4 2 1 .3 4 4 a .i t 1 .3 4 9 1 .3 5 2 1 .3 5 4 1 .3 5 7 1 .3 5 9 1 .3 6 2 1 .3 6 5 1 .3 6 7 1 .3 7 0 1 .3 7 2 1 .3 7 o 1 .3 7 1 .3 8 0 1 .3 8 2 1 .3 8 5 1 ..7 8 7 4 .9 0

4 .9 1 4 .0 2

4  i f ‑;

4 .9 4 4 .9 5

1 .3 9 0 1 ‑3 9 2 1 .3 9 5 1 .3 9 8 1 .4 0 0 1 .4 0 2

(94) ^{Mitsuo TAKAHASHI}

x .      ¥   y ‑ ‑  x 蝣一蝣".  v >  N ‑.

4 . 9 6  1 .4 0 5 4 .9 7  1 .4 0 8

5 .4 fi    1 .5 3 1     5 .9 6    1 .6 5 6     6 .4 6    1 . 7 8  1 5 .4 7    1 . 5 3 3     5 .9 7 L 6 5 8 6 . 4 7 ] . 7 8 :;

4 .9 S    1 .4 1 0    5 .4 *    1 .5 3 6    5 .9 S    1 .6 6 1    6 .4 8 1 .7 8 5 6 . 4 9

6 .5 0

6 . 5 1  1 . 7 8 7] .7 i> 01 .7 !ｻ2

5 .0 2    1 .4 2 0    5 .5 2    1 .5 4 6    6 .0 2 1 .H 7 1 fi. 3 2 1 .7 !.) '>

5 .0 3 5 .0 4

5 .0 5  1 .4 2 31 .4 2 51 .4 2 8  5 .5 45 .5 5  1 . 5 4 Sl .5 5 11 .5 5 4  6 .0 36 .0 46 .0 5  1 .6 7 61 .6 7 31 .6 7 8 5 .0 6  1 .4 3 0  5 .5 6  1 .5 5 6  6 .0 6  1 .6 8 1 H . 5 ‑J

6 .5 4 6 . 5 5 tt .a fi

1 .7 IJ 7 1 .K O O 1 .8 0 2

*  l .S O 5 5 . 0 7     1 .4 S¥ 3 o .o  i     1 . 5 5 9     6 .0 7    ] .H 8 3 fi . 5 7     1 .8 0 7 5 .0 8    1 .4 3 5    5 .5 8    1 .5 6 1    6 .0 8    1 .6 3 6    6 .5 8    1 .8 0 9 5 .0 9  1 .4 3 8

5 .1 0  1 .4 4 0

・

5 . l l  1 .4 4 ‑, 5 .1 2  1 .4 4 5 5 .1 8  1 .4 4 8 5 . 1 4  1 .‑J 5 0 5 . 1 5  1 .4 5 3 5 . 1 6  1 . 4 5 5 5 .V ,  1 . 4 5 8 5 . 1 8  1 .4 6 1 5 .1 9  1 .4 6 3 5 . 2 0  1 .4 6 6

5 .5 9    1 .5 6 4     6 .0 9 1 .6 8 8     6 .5 9 1 .8 1 2 6 .6 0  1 .8 1 5 6 .6 1  1 .8 1 1;

fi .6 2  1 .8 2 0 fi .fi S  1 .8 2 3 Q f A  1 .8 2 5 6 .6 5  1 .8 2 7 fi .fi fi  1 .8 2 9 5 .6 7    1 .5 8 4     fi .1 7    J . 7 0 8     H .H 7    1 .8 8 2 S . fi S     1 .5 8 6     6 .1 8     1 .7 1 1 H .fi S L 8 .j 4

5 . 6 9     l .o s y     a 1 9 1 . 7 1 .J 6 .n y     1 .8 .'J 7 5 .7 0    1 .5 9 1     6 .2 0    1 .7 1 6 6 .7 0 1 .8 4 0

5 .2 1  1 .4 6 8 5 .2 2  1 .4 7 1 5 .2 3  1 . 4 7 3 5 . 2 4  1 .4 7 6 D .'i o  1 .4 7 8 5 .2 6  1 .4 8 1

5 . 7 1    1 .5 9 4     6 .2  1    1 . 7 1 8     ｫ .7 1    1 .8 4 2 5 . 7 2    1 .5 9 6     fi .2 2    1 . 7 2 1     H .7 2 1 .8 4 4

1 .5 9 9     6 .2 3 1 .7 2 8 0 .7 8 1 .8 4 7 5 . 7 4    1 .5 0 2    6 .2 4    1 .7 2 (i     ｫ .7 4    1 .8 4 9 5 .7 5  1 .5 0 4  6 .2 5  1 .7 2 8

5 . 7 6  1 .5 0 7  6 .2 6  1 . 7 3 1

6 .7 5  1 .8 5 2 fi .T H  1 .8 5 4 5 .2 7    1 . 4 8 'J          1 .6 1 0     B .2 7 1 . 7 3 31 .7 3 5   8 .7 76 .7 8  1 .8 5 71 .8 5 9 5 . 2 8     1 .4 8 6 5 .7 8     1 .6 1 2     6 .2 8

5 .2 9    L 4 8 8    5 . 7 9    1 .6 1 4 '    fi .2 9    1 . 7 3 7    6 .7 9    1 .8 6 1 5 .3 0  1 . 4 9 1

5 .3 1  1 . 4 9 3 5 .8 2  1 .4 9 6 .').o i  l . ‑m s

O . i * 5 .8 0

5 .8 1  l .O H1 .6 1 9  l .6 1 6 6 .3 06 .3 1  i . < ・> il . 7 3 ｻ1 .7 4 .3  6 .8 06 .8 1 1 .8 8 4 ] .8 6 fi 1 .6 2 11 .8 2 41 .8 2 81 .6 2 91 .6 3 11 .8 3 4  6 .3 26 .3 36 .3 46 .3 56 .3 66 .3 7 6 .8 2 1 .8 6 9

6 .X .S  1 .8 7 1 ｫ .ｫ 4  1 .8 7 4 6 .8 5  1 .8 7 H B .rt ti  1 .N 7 9 (1 8 7  ] > 8 1 5 .8 4

5 .8 5  1 . 5 0 11 . 5 0 8

5 .:;ｫ  l .? >0 6

o .o  I    1 . 5 0 S     5 .8 7    1 .H 3 4     6 .o T l .T o S

1 .5 1  1    5 .S 8    1 .6 3 6    (5 .3 8    1 .7 6 0    6 .8 8

5 .8 9    1 .5 1  3     5 .S D    l .fv > *     fi .3 9    1 . 7 6 3 fi .S O  ] .8 S rt tf .H O  1 .8 8 'J H .il l  1 .8 9 1 5 .4 0  1 . 5 1 6

..4 1  1 . 5 1 8 5 .4 2  1 .5 2 1

5 . 9 0

・

5 X l  l .ru il .6 4 .3  n . ‑)V6 .4 06 .4 1  J . i n ‑>1 .7 6 51 . 7 6 s

5 .9 2    1 .6 4 fi     (5 .4 2    1 .7 7 0 ｫ .'J 2  1 .8 9 :5 6 .y .)  1 .8 9 6 5 .4 3    1 .5 2 3     5 .9 3    1 .6 4 8     fi .4 3    1 . 7

(95)

N / l o o N a .,S o O .

  c u

x y l o s er u s :  N a 2 S 2 O sN / i o oc c N / i o o         N / l o o

X y lo s  m gｻ  X .      N .  ‑ . ' '    X  一 蝣

H M 6 .y ; 6 . 9 8 (3 .W 7 .0 0 7 .0 1 7 .0 2 7 .0 3 7 .0 4 7 .0 5 7 .O fi 7 .0 7 7 .0 .S 7 .0 9 '. 1 0 r .n

 1 .U 1 3  7 .2 0 1 .9 1 6  7 .2 1 ] .9 L S  7 .2 2 1 .9 2 0  7 .9 3 1 .0 2 'J  7 .2 4 1 .9 2 5  7 .2 5 1 .9 2 ‑S  7 .2 ｫ 1 .9 3 0  7 .2 7 i .tfe v ;  7 .2 8 1 .9 .'J 5  7 .2 9 1 .9 3 3  7 .3 0 1 .9 4 0  7 .3 1 7 . 1 2

U 3 r. 1 4 '. 1 5

1 .9 4 2  7 .3 2  1 .9 9 1 1 .9 4 5  T .3 3  1 .9 9 4 1 .9 4 7    1 .1 11 )6 1 .9 5 0  7 .3 5  l .'J 'J 'J

Summary

The author has already reported a micro method for the determination of

xylose. The author has made the calculating table so that it may de possible to obtain milligrams of xylose dirreetly from the difference ill titration between the blank and the sample.

Liter atures

1. Takahashi, M. : J. Nara Gakugei University, 2, 191 (1953) 2. Takahashi, M., and Tanaka, I.:ibid. 3, 219 (1953)

3. S'l'ifKs, II. K., Petkksok, W. II., and Fkkd E. B.:J. Baet. 12, 427 (1926)