• 検索結果がありません。

Serial Analysis of Gene Expression in Human Gastric Cancer

N/A
N/A
Info
ダウンロード
Protected

Academic year: 2022

シェア "Serial Analysis of Gene Expression in Human Gastric Cancer"

Copied!
1
0
0

読み込み中.... (全文を見る)

今ダウンロードする ( 1 ページ )

全文

(1)Serial Analysis of Gene Expression in Human Gastric Cancer Kaname Yamashita1), Hidekazu Kitakata1), Kazuo Yasumoto1), Kazuhiko Omote1), Yutaka Takahashi1) Toshinari Minamoto2) (Div. of Surg. Oncol1). and Diag. Mol. Oncol2)., Cancer Res. Inst., Kanazawa Univ.) It is important to identify genes responsible for metastasis of gastric cancer, as this disease is one of the leading causes of cancer death in the world. Here we analyzed gene expression in human gastric cancer by serial analysis of gene expression (SAGE). SAGE is a method for comprehensive analysis of gene expression patterns. A short sequence tag (10-14bp) contains sufficient information to uniquely identify a transcript. The tag is obtained from a unique position within each transcript. Sequence tags can be linked together to from long serial molecules that can be cloned and sequenced and quantitation of the number of times a particular tag is observed provides the expression level of the corresponding transcript. We obtained primaly tissue, normal mucosa, and lymph node metastasis from a advanced gastric cancer patient. Total RNA was isolated and SAGE was performed. Tag sequences were analyzed using SAGE software. A total of 60621 transcripts were identified. We show tags which highly expressed, in primaly tumor, normal mucosa, and lymph node metastasis. The lymph node metastasis library was compared with primaly tumor library, we could identify certain set of genes up-regulated in lymph node metastasis in tag ratio of two fold or more. Validation by RT-PCR on RNA from another patients shows higher expression of SIAT6, APOC1, galectin-1 and COL1A1 in lymph node metastasis versus primaly tumor. These results together with currently accumulating data would provide basic knowledge toward understanding molecular mechanism underlying progression and metastasis of gastric cancer. Summary of SAGE tag libraries Library. Sequences. Total Tags. Unique Tags. Normal. 731. 14843. 5243. Tumor. 933. 22681. 8337. 1030. 23097. 7976. LN meta. ― 97 ―.

(2)

参照

今ダウンロードする ( PDF - 1 ページ - 123.32 KB )

関連したドキュメント

―依存傾向と生活習慣について―

A tendency toward dependence was seen in 15.9% of the total population of students, and was higher for 2nd and 3rd grade junior high school students and among girls. Children with

Development of Radiopharmaceuticals for Diagnosis and Therapy of Metastatic Bone Cancer

Moreover, based on the similar concept, we designed, synthesized, and evaluated a 99m Tc-6-hydrazinopyridine-3-carbox- ylic acid-conjugated bisphosphonate ( 99m Tc-HYNIC-HBP) as a

Adiponectin receptor-1 expression is associated with good prognosis in gastric cancer

Methods: In this study, we evaluated expression levels of 2 adiponectin receptors – AdipoR1 and AdipoR2 – and attempted to correlate their expression with prognosis in gastric

An emerging strategy for cancer treatment targeting aberrant glycogen synthase kinase 3 β

A putative pathological role for GSK3β in cancer was demonstrated by our observations that its inhibition by pharmacological (small-molecule) inhibitors and RNA interference

Glycogen synthase kinase-3β is a pivotal mediator of cancer invasion and resistance to therapy

Abbreviations: AR, androgen receptor; BDNF, brain-derived neurotropic factor; DM, diabetes mellitus; DR4, 5, death receptor 4, 5; EGF, epidermal growth factor; GSK3β, glycogen

Inhibition of GSK-3β activity attenuates proliferation of human colon cancer cells in rodents

Groups of four or five athymic mice (Balb/c, nu/nu) with subcutaneous xenografts of SW480 human colon cancer cells were treated with dimethyl sulfoxide (DMSO) or different doses (1,

Expression and subcellular localisation of AID and APOBEC3 in adenoid and palatine tonsils

Moreover, we divided patients into two groups and compared the expression levels of AID/A3s in the adenoids and palatine tonsils between both groups using the Mann–Whitney U test:

Detection of sentinel lymph node using contrast-enhanced agent, Sonazoid (TM) , and evaluation of its metastasis with superb microvascular imaging in oral and oropharyngeal cancers: a preliminary clinical study

Contrast-enhanced ultrasonography, oral cancer, oropharyngeal cancer, sentinel lymph node, Sonazoid TM , Superb Microvascular