Acta Medica Okayama
Volume 37, Issue 1 1983 Article 4
F EBRUARY 1983
In vitro transformation of adult rat liver cells by 3’-methyl-4-dimethylaminoazobenzene.
Syarifuddin Wahid ∗
∗Okayama University,
Copyright c1999 OKAYAMA UNIVERSITY MEDICAL SCHOOL. All rights reserved.
In vitro transformation of adult rat liver cells by 3’-methyl-4-dimethylaminoazobenzene. ∗
Syarifuddin Wahid
Abstract
Primary cultures of liver cells from normal adult rats were treated with 3’-methyl-4-dimethylaminoazobenzene (3’-Me-DAB) at various concentrations for 6 days. 3’-Me-DAB treatment induced rapid prolifera-
tion of epithelial clear cells with chromosomal abnormalities and gamma-glutamyl transpeptidase (GGT) activity. In early culture, marker chromosomes were detected in 13 of 44 3’-Me-DAB- treated cultures but not in control cultures. GGT activity was not detected in the epithelial clear cells in either 3’-Me-DAB-treated or control cultures. In late culture, 21 cell lines established from 39 carcinogen-treated cultures consisted of 3 diploid cell lines, 5 pseudodiploid cell lines and 13 aneuploid cell lines. Eighteen of these 21 cell lines had marker chromosomes. Of the 2 cell lines established from 15 control cultures both were aneuploid, but a marker chromosome was detected in only one of these. GGT activity was detected in 11 of 21 cell lines established from the carcinogen-treated cultures but not in those from control cultures. Morphological fea- tures of the cell lines which varied from normal to cancerous included polymorphism, increased nuclear/cytoplasmic ratio and prominent nucleoli. No cell line established in this study developed tumors in host rats during a 1-year observation period.
KEYWORDS:primary liver cell cultures, 3’-methyl-4-dimethyl-aminoazobenzene, proliferation of epithelial clear cells, chromosomal abnormality, gamma- glutamyl transpeptidase
∗PMID: 6133413 [PubMed - indexed for MEDLINE]
Copyright (C) OKAYAMA UNIVERSITY MEDICAL SCHOOL
1 Wahid: In vitro transformation of adult rat liver cells by
Produced by The Berkeley Electronic Press, 1983
2 Acta Medica Okayama, Vol. 37 [1983], Iss. 1, Art. 4
http://escholarship.lib.okayama-u.ac.jp/amo/vol37/iss1/4
3 Wahid: In vitro transformation of adult rat liver cells by
Produced by The Berkeley Electronic Press, 1983
4 Acta Medica Okayama, Vol. 37 [1983], Iss. 1, Art. 4
http://escholarship.lib.okayama-u.ac.jp/amo/vol37/iss1/4
5 Wahid: In vitro transformation of adult rat liver cells by
Produced by The Berkeley Electronic Press, 1983
6 Acta Medica Okayama, Vol. 37 [1983], Iss. 1, Art. 4
http://escholarship.lib.okayama-u.ac.jp/amo/vol37/iss1/4
7 Wahid: In vitro transformation of adult rat liver cells by
Produced by The Berkeley Electronic Press, 1983
8 Acta Medica Okayama, Vol. 37 [1983], Iss. 1, Art. 4
http://escholarship.lib.okayama-u.ac.jp/amo/vol37/iss1/4
9 Wahid: In vitro transformation of adult rat liver cells by
Produced by The Berkeley Electronic Press, 1983
10 Acta Medica Okayama, Vol. 37 [1983], Iss. 1, Art. 4
http://escholarship.lib.okayama-u.ac.jp/amo/vol37/iss1/4
11 Wahid: In vitro transformation of adult rat liver cells by
Produced by The Berkeley Electronic Press, 1983
12 Acta Medica Okayama, Vol. 37 [1983], Iss. 1, Art. 4
http://escholarship.lib.okayama-u.ac.jp/amo/vol37/iss1/4
13 Wahid: In vitro transformation of adult rat liver cells by
Produced by The Berkeley Electronic Press, 1983
14 Acta Medica Okayama, Vol. 37 [1983], Iss. 1, Art. 4
http://escholarship.lib.okayama-u.ac.jp/amo/vol37/iss1/4
