ɜለኴˤ̎˞ɝ ɉɉఙ ɉAutometallographyʎӟᇍ֊ࠜʱড়ᄍɶɾᎻᐨᎲᓞ֊ࠜʍ൮ԎຫʆᶬҸᅉ೮ᕫຫʍimmunogold-silver೮ᕫຫʣᎻᐨӑᨅ࢙൮Ԏʍሗ֊ᩂຫʉʈʆᄍɣʨʫʪɋAutometallographyʍטড়ʇɶʅЋ ᄍɸʪႻ҈ʊʎвᠷˉ̃ʺ˟ʇɶʅ፲ኙ৷ʍɡʪʸ˿˩ʸˊ˶ʍЋᄍɫ᮶ʆɡʪɫᶬපɍʉሀ ɫɡʪʍʆᶬвᠷˉ̃ʺ˟ʍϐಌၑᢑʇɶʅށშၤ֊ၑʍ˅˿ʼ̉ʾ̎˜́ʍ৷ᢑʊᇗᆾɶʅႻ҈ ʍᕩʱោʞᕩުʉીೖʱৃɾɋ ˃̎̅̎˟ᶺᎻᐨ֊ࠜᶬᩂ݇ຫᶬႻ҈ᶬᨁᨅ࢙ᶬ˅˿ʼ̉ʾ̎˜́ ʎɷʠʊ ɉImmunogold-silverຫʣᨁᨅ࢙ʍᎻᐨ֊ࠜᆔ൮ԎຫʆᄍɣʨʫʪautometallographyʍႻ҈ʎၑ ႾႻ҈ʇʡئʏʫᶬᩂܽᶬҮԺᶬвᠷˉ̃ʺ˟ᶬpHጱԺʆԂఄɴʫʪɋҮԺɫᙪʆɡʪ ֊ࠜႻ҈ʇʎᄴʉʩᶬၑႾႻ҈ʎႻ҈ͼʊᩂܽʇҮԺʱ؉ʲʆɣʪɲʇʊၔɫɡʪɋᩂܽ ʇɶʅʎሕ᧦ᩂᷛ3ᶬ4ᷝᶬ᧦ᩂᷛ4ᶬ6ᷝᶬΖ᧦ᩂᷛ1ᶬ4ᷝʉʈɫԢᄍɴʫʅɣʪɫᶬɣɹʫʍᩂܽ ʱᄍɣʪܬʆɡʂʅʡᶬাʍᩂܽʍ৵ᥴʆᦋԿʉҮʱหɸʪɾʠʊႻ҈ʍીԔʊвᠷˉ ̃ʺ˟ʱՒɧʪɫɡʩᶬвᠷˉ̃ʺ˟ʎႻ҈ʍࠪ࠳৷ᶬautometallographyʍдᯈ৷ʉʈʱं ױɸʪɋূಿЋᄍɴʫʅɣʪʸ˿˩ʸˊ˶ʎҥʫɾвᠷˉ̃ʺ˟ʆɡʪɫපɍʉดʡɡʩᶬɲʫ ʊϐʮʪၑᢑɫᫎɮʠʨʫʅɣɾᷛ4ᶬ9ᷝɋಢለኴʆʎᶬདͼɪʨᨅ࢙ʺˀ̉ʱԔɸʪʍʊԢ ᄍɴʫʅɣʪށშၤ֊ၑʍ˅˿ʼ̉ʾ̎˜́ʊᇗᆾɶᶬ௦ኚʍ˅˿ʼ̉ʾ̎˜́ɪʨ18ŻCrownŻ6 ᶨFig. 1ᶩʱ᧦ᩂʍႻ҈ʊࡶӁɶʅంɾʉႻ҈ʍЀીʱោʞɾɋᒑഛɶɾᕩႻ҈ᶨ˅˿ʼ̉ ʾ̎˜́Ⴛ҈ᶩʱᨁᨅ࢙ʍᎻᐨ֊ࠜᆔ൮Ԏຫʆɡʪሗ֊ᩂຫʊᦞᄍɶʅᶬautometallographyຫʍ ᕩʇΩᨸʍᎻᐨ֊ࠜᆔ൮ԎʊᕩުʉᏃೖʱৃʪɲʇɫʆɬɾɋ
ɹʳɰʽɲ˂ʐʵɥႊȗȲ
Autometallography းЅ
1ɉפࠜᬎށࠜɉ̀˥˩̀˜̎ˍ˽̉ࠜᧅ Correspondence to Osamu FujimoriE-mail: [email protected]
Received 8 January, 2021 Accepted 10 January, 2021
ᙸɉ൛ɉɉɉи
1ಲ௶ʇఄຫ Ꮋᐨಲ௶ ɉթၑʎSD˿˙˞ᶨᭀᶬ15ϑᶩ3ᯃʱɼʫɽʫ˓˶ˤ˰̉˗́ʍᔉᓾͮʊʧʩࠪඕึɴɺᶬ ᓥাᥴʣɪʊं࠺ʧʩ0.5ᶥሗ֊ˠ˞̀ʼ˶̍4ᶥ paraformaldehyde PBSᶨ0.1MᶬpH7.4ᶬ4ƈᶩད 50mlʆ࿋໐ۓ࠳ɶɾɋᓵɩʧʒᎃऀͫϹʱԎɶᶬɴʨʊۓ࠳ʊʅͥ4ƈʆཷۓ࠳ɶɾ ʍʀᶬPBSʆ24ీ໑ᶬ࠳ຫʊɶɾɫʂʅ˧˿˫ʹ̉ւɶᶬཛྷॾ˵˅̃˞̎˶ʆ˧˿˫ʹ̉ ԕ၄ᶨ4īᶩʱЀીᶬˏ˿ʺ˟ˆ˿ˏʊϊɶʅ˭̂˧˿̎˞ಢʇɶautometallographyʊЙɶɾɋ ɉʉɩಢለኴʎפࠜᬎށࠜթၑ࠷߆ىϥʍ૭៖ʱৃʅᜓʂɾᶨឯᄰ׳2010Ż012ᶩɋ Âöõðîæõâííðèóâñéú ɉ˅˿ʼ̉ʾ̎˜́᧦ᩂႻ҈ʎ18ŻCrownŻ6ᶬ᧦ᩂᶬ˥ʺ˟̃˃ˤ̉ᶬ˅ʾ̉᧦ᐂʆԂఄ ɴʫʪɋႻ҈ʎɡʨɪɷʠЀીɶɾϒͬʍAʇBʱЋᄍᇀԳʊ༌ɶʅЀીɶɾɋ Aᶺ ᘛᄤᶨ2ۋᘛᄤᶩ 25 ml 18ŻCrownŻ6 ᧦ᩂ Bᶺ 0.2 Mɉ˅ʾ̉᧦ᐂᶨpH3.75ᶩ 25 ml ˥ʺ˟̃˃ˤ̉ 0.2 Mɉ˅ʾ̉᧦ᐂᶨpH3.75ᶩ ƌɉ0.2Mɉ˅ʾ̉᧦ད 21 ml ƍɉ0.2Mɉ˅ʾ̉᧦ˠ˞̀ʼ˶ད 9 ml ᶨƌʝɾʎƍʆpHʱ3.75ʊ௨ɸʪᶩ ɉႻ҈50 mlͼʍ18ŻCrownŻ6ᶬ᧦ᩂᶬ˥ʺ˟̃˃ˤ̉ʍɼʫɽʫʍᨃʱᜟ1ʍʧɥʊឮ࠳ɶᶬ 64ᥱʩʍោᙪᨃʍᎻʞʮɺʆႻ҈ʱЀીɶʅautometallographyʱᜓɣᶬɼʫɽʫʊʃɣʅႻ҈ ʇɶʅʍᦞ؇ʱ൮ឡɶɾɋ ᜟ1 18ŻCrownŻ6 12.5 mg 25 mg 50 mg 100 mg ɉ᧦ɉᩂɉ 25 mg 50 mg 100 mg 200 mg ˥ʺ˟̃˃ˤ̉ 50 mg 100 mg 200 mg 400 mg ɉႻ҈ʍԟ࠳ʎᎻᐨԕ၄ʊautometallographyʱᜓɥʇʇʡʊᶬᨅ࢙ᩂʍᮂၔᄴᆔԎʊʧʪႻ҈ ʍՓ֊ʱᒿᇖᆔʊឍࡗɶʅᶬɼʫʨʍᏃೖɪʨᦞԕʉោᙪʍʉʨʒʊႻ҈ʍᦞ؇ʱԟ࠳ɶɾɋʉ ɩᶬautometallographyఆᜓাʍᎻᐨԕ၄ʎˇ́̉ʾ˨˞̃̎˞ᶨkernechtrot, Nuclear fast redᶩʊ ʅ೮ᕫʱఆɶ˦́ˋ˶Ӂɶɾɋ
ɉႻ҈ʍ௨ʎ࠺ӑဆనͬʆᜓɣᶬ௨াᇀʀʊႻ҈ʊЙɶɾɋႻ҈ʎ೮ᕫჸʱለኴ࠺ӑʍဆనͬ Çê诡²ɉCrown Etherᶨ18ŻCrownŻ6ᶩ
ʆ˅̎˿̎˲˙˅ˏӑʊᑝɣʅᦠҴʍͬʆᜓʂɾɫᶬႻ҈ʍҴʊࡩɸʪࠪ࠳৷ʱʘʪɾʠʊ࠺ӑ ဆనͬʆʡႻ҈ʱᜓʂɾɋ ɉ18ŻCrownŻ6ʉʨʒʊ᧦ᩂʎSigmaʍᝒقʱЋᄍɶɾɋHackerʨɫୟޢɸʪFLUKAʍ᧦ᩂʎ 60ƈʍ༽ʆ15ԔଏɶʉɣʇདពɶʉɣʍʊࡩɶᶬShigmaᝒʎण༦ʆపད৷ʆɡʩЋᄍɶʣɸ ɪʂɾɋ˥ʺ˟̃˃ˤ̉ʎࡎݥ˫ʹ́˶ᶬ˅ʾ̉᧦ʎحҴᎣᙪʍᝒقʱᄍɣɾɋ ɉʝɾ˅˿ʼ̉ʾ̎˜́Ⴛ҈ʊʧʪᏃೖʎʸ˿˩ʸˊ˶ʱЋᄍɸʪHackerʨʍ᧦ᩂႻ҈ᷛ6ᷝ ʊʧʪautometallographyʍᏃೖʇʡ๚ᤑɶɾɋ Ꮓೖ ɉ18ŻCrownŻ6ʎ᧦ᩂႻ҈ʊɩɣʅҥʫɾвᠷˉ̃ʺ˟ප՞ೖʱᆌ୷ɸʪɲʇɫሯɪʠʨʫɾɋ ᧦ᩂᨃɫ100 mgᶬ200 mgʆ18ŻCrownŻ6ᨃɫࡸᨃʍܬᶬטড়ɫచɣɾʠʊሀీʆႻ҈ɫࠬΛ ɸʪɫᦞႻ҈ও्ʱᢳɧʅᦋႻ҈ʊᬑʩʣɸɪʂɾɋʝɾႻ҈ʍሀీʆʍՓ֊ɫᒿᇖᆔʊʡన ʨɪʆɡʩᶨFig. 2Ż3ᶩᶬᮂၔᄴᆔʉᨅ࢙ᩂʍᎻᐨᶬˏ˿ʺ˟ˆ˿ˏʉʨʒʊ೮ᕫჸʗʍຌᇗ̍೮ʱ ᢰɲɶʣɸɪʂɾɋɲʫʨʍดʎ18ŻCrownŻ6ʱ݇ᨃɸʪɲʇʊʧʩٴɴʫɾᶨFig. 2Ż3ᶩɫᶬ ɼʫʊড়ɷʅႻ҈ʊʡీʱɸʪʧɥʊʉʩᶬাᥙɸʪᦞԂఄʍႻ҈ʇɮʨʘԢʎɶɪʂ ɾɋ ɉͥఄᶬ᧦ᩂɫ12.5 mgʆ18ŻCrownŻ6ɫࡷᨃʍܬᶬႻ҈ʍᒿᇖᆔՓ֊ʎᫎీʊʮɾʂʅ ʨʫʉɣʡʍʍ1ీϒͫʍႻ҈ʆʡ֝Ԕʉטড়ও्ɫৃ᭐ɮᶬᩂܽʍᏒࡩᨃɫͭᢷɶʅɣʪʡʍ ʇᒑɧʨʫɾɋ᧦ᩂ25 mgʍܬʎᶬɲʫʧʩʡٴɴʫʪʡʍʍᦞঞᨃʇʎԟఀʆɬʉɪʂɾ ᶨFig. 2Ż3ᶩɋ ɉʉɩͥᕓᆔʊ᧦ᩂႻ҈ʆʎᶬሕ᧦ᩂႻ҈ᷛ3ᶬ4ᶬ5ᷝʆʎʨʫʪɲʇɫʉɪʂɾžᩂ᪥טড়ſ ʊʧʪʊʧʪ೮ᕫჸʍ೮ʱᢰɲɶʣɸɣ҂ɫɡʂɾɋ೮ᕫჸʍ೮ʎ҈ͼʗʍᨅ࢙ᩂʍᮂၔᄴ ᆔຌᇗʱ៚ᆌɶʅႻ҈ʍՓ֊ʱచʠʪɫᶬ೮ᕫჸʍӑᮅʱ5ᶥ။ᜏ༒ʸ́ˬ˵̉དʆˉ̎˞ɶᶬ Ԕʱԕʂɾাᶬ60ƈʆͥΗဤɴɺʅЋᄍɸʪʇɲʍʧɥʉ೮ʱหɸʪ՞ೖɫɡʂɾɋ ɉោᙪᨃʱኚɍʊݳɧʅ൮ឡɶɾᏃೖᶬႻ҈ʍᎻીʇɶʅʡᦞঞʆɡʂɾʍʎϒͬʍᥱʩʆɡʂ ɾɋɲʍႻ҈ʱᨅ࢙Ꮋᐨ֊ࠜʍautometallographyʊᦞᄍɶᶬʸ˿˩ʸˊ˶ʱЋᄍɸʪHackerʍ ᧦ᩂႻ҈ʊʧʪᏃೖʇ๚ᤑɸʪʇᶬ60ԔʉɣɶɼʫϒͫʱɶɾႻ҈ీʎ15 ᷾ 20Ԕʊሀᐗɴʫᶬ Ⴛ҈ʍЀીᶬႻ҈াʍ໑ʉʈʡඐʠʅࡄపʆɡʂɾɋ A 18ŻCrownŻ6 25 mg ᧦ᩂ 50 mg ᘛᄤ 25 ml B ˥ʺ˟̃˃ˤ̉ 200 mg ˅ʾ̉᧦ᐂᶨpH3.75ᶩ 25 ml ɉʉɩಢႻ҈ʆᦠҴɺɹ࠺ӑҴʍͬʆႻ҈ʊЙɶɾܬᶬၔᄴᆔʉႻ҈טড়ʊɸʪీʎᦠҴͬ
ɣɹʫʡ18ŻcrownŻ6 25 mgᶬ˥ʺ˟̃˃ˤ̉ 200 mg ʆᶬंɪʨ᧦ᩂ 12.5 mgᶬ25 mgᶬ50 mgᶬ100 mgᶬ200 mg ʆԂఄɶɾႻ҈ɋ Fig. 2ɉ᧦ᩂ 100 mg ʇ 200 mg ʍႻ҈ʊʎᮂၔᄴᆔʉᨅ࢙ᩂʍԎʊʧʪྠʩɫԎʅɣʪɋ Fig. 3ɉ ᧦ᩂ 100 mg ʇ 200 mg ʍႻ҈ʎᮂၔᄴᆔʉᨅ࢙ᩂʍԎʆᵋ֊ɶᶬ೮ᕫჸʊᩂ᪥טড়ɫ ʨʫɾɋ᧦ᩂ50 mg ʍႻ҈ʆʡ 15 ᷾ 20 Ԕʝʆʊᐂিʊᮂၔᄴᆔʉᨅ࢙ᩂʍԎɫᢰɲʩ೮ ᕫჸʗʍϊᇗɫʨʫʪɫᶬɲʍ์ᬤʆʎҴᯑ̂˯́ʆᎻᐨʍᮂၔᄴᆔʉᨅ࢙ᩂʍຌᇗʎʚʇ ʲʈ៖ʠʨʉɪʂɾɋ Çê诡³ɉᦠҴͬ5 ԔᏀᦋ Çê诡´ɉᦠҴͬ20 ԔᏀᦋ ໟᰚɪʨัၤۋʊɩɰʪΩᨸʍࢋۦɫᨅ࢙ᩂטড়ᄊၑʇɶʅᵋᕫʊ៖ʠʨʫʪɋ Çê诡µɉ˿˙˞ᓵᶨໟᰚᶩ Çê诡¶ɉ Fig. 4 CA4 ᮾܕʍଞށ ᎃऀͫϹጫͫᆣʍᎲᓞ᮲ͫᧅʊΩᨸʍࢋۦʱቌɸטড়ᄊၑʍᭂከɫ៖ʠʨʫʪɋ ጫᓾӑʊʎᏺᏢၤʊݼ௦ʍᎃࠍɫʨʫʪɋ Çê诡·ɉ˿˙˞ᎃऀͫϹ
ʍܬʇපʆɡʪɫᶬႻ҈ʍՓ֊ɫచ৵ʊʟɾʠᮂၔᄴטড়ʉʈʱঃɬᢰɲɶʣɸɪʂɾɋ ɉρۋʍ൮ឡʍᏃೖᶬ˅˿ʼ̉ʾ̎˜́ʱᄍɣɾႻ҈ʎЀીɫࡄపɪʃ೮ᕫЀʡፍУʆɡʩᶬূ ಿʍఄຫʧʩʡሀీʆᮂၔᄴᆔטড়ʍʚʇʲʈʉɣsilver enhancementɫᓧʇʉʂʅᶬໟᰚɩʧ ʒᎃऀͫϹʍΩᨸʍࢋۦʱূಿʍఄຫʇපʊ൮ԎɸʪɲʇɫʆɬɾᶨFig. 4Ż6ᶩɋ ʝʇʠʇᒑࡗ ɉၑႾႻ҈ʎႻ҈ͼʊᩂܽʇҮԺʱ؉ʲʆɣʪʍʆᶬႻ҈ʍাʎᇀʀʊҮԺɫᩂܽʱ ҮɶʅႻ҈ͼʆᨅ࢙ᩂʍথીɫ߂ʝʩᶬႻ҈ʇʉʪᨁᨅ࢙ʊᨅ࢙ᩂɫຌᇗɸʪɋɶɪɶᩂܽ ɫ৵ྟɪʃᦋԿʊҮɴʫʪʍʆᶬɲʫɫᮂၔᄴᆔטড়ᄊၑʇʉʩᬝ৷טড়ʇʍᠪԠɫۏ᭐ʊʉʪɋ ᩂܽʍ৵ྟʉҮʱหɸʪɾʠʊၑႾႻ҈ʊʎвᠷˉ̃ʺ˟ɫᄍɣʨʫʅɩʩᶬLüppo-Cramer ᶨ1914ᶩᷛ7ᷝϒಿᶬвᠷˉ̃ʺ˟ʇɶʅʸ˿˩ʸˊ˶ɫЋᄍɴʫʅಿɾɋʸ˿˩ʸˊ˶ʎᶬɼʍ፲ኙ ৷ʊʧʩႻ҈ͼʊ؉ʝʫʪᩂʺˀ̉ʇ˥ʺ˟̃˃ˤ̉ʉʈʍҮԺʇʍטড়ʱᐂিʊʠʪɋɲʍ ՞ೖʊʧʂʅ৵ྟʉᩂʺˀ̉ʍҮʱԧɶʅᮂၔᄴᆔʆ፱ށʉᨅ࢙ᩂʍԎʱหɶᶬၔᄴᆔʉᬝ ৷ᧅϴʍʞʊ্Ꮂʆۮᢑʉᨅ࢙ᩂɫᐂʣɪʊথીɴʫʪɋ ɉ൧ၑʍʸˁˍʸʍේʱۓʠɾʸ˿˩ʸˊ˶ʎނᎫಲʆɡʪɾʠીԔʍᎻીɫͥ࠳ɶʅɣʉɣᶬ ࠷ᄍྡྷ्ʍདʊདពɶʄʨɣᶬͭད৷ၑᢑɫ؉ʝʫʅɣʪʍʆɲʫʨʱᬐאɸʪɫɡʪᶬ፲ ኙʆɡʪɾʠႻ҈াʍ໑ʆᬐאɶʊɮɣʉʈʍดɫɡʩᶬՒɧʅвᠷˉ̃ʺ˟ʇɶʅಐᄍʉɼʍ ፲ኙ৷ʥɧʊטড়াʊႻ҈ɫ໑ɶʊɮɣʇɣɥሀʡɡʪɋીԔɫۮᢑʆԂఄɶʣɸɣϐಌោᙪ ɫɲʫʝʆʊʡɣɮʃɪ൮ឡɴʫʅɬɾɫᶬʸ˿˩ʸˊ˶ʊϐʮʪвᠷˉ̃ʺ˟ʎɣԎɴʫʅɣʉ ɪʂɾᷛ5ᶬ9ᷝɋρۋᶬᗾᒓʎূಿʍʸ˿˩ʸˊ˶ʊϐɧʅ˅˿ʼ̉ʾ̎˜́ʱႻ҈ʊࡶӁɶʅ ᕩʱោʞᶬ˅˿ʼ̉ʾ̎˜́Ⴛ҈ʍautometallographyʊɩɰʪಐᄍ৷ʱ൮ឡɶɾɋ ɉ˅˿ʼ̉ʾ̎˜́ᷛ8ᷝʎԔࠍӑʊࠏʱಐɸʪށშၤ֊ၑʆᶬࠏসʊᦞɶɾʺˀ֤̉সʱ ପʃᬝʺˀ̉ʱᦦ૾ᆔʊכʩ᥈ʲʆࠪ࠳ʉ᩺Ϲʱথીɸʪၑᢑʆɡʩᶬʺˀ̉ʍᝆᢷᶬԔᶬྡྷᐗʉ ʈʊԢᄍɴʫʪɋࠏসʎ˅˿ʼ̉ʾ̎˜́ʍኚᯕʊʧʂʅᄴʉʩᶬᩂʺˀ̉ʍ֤সᶨ1.26Ƌᶩʇ ࠏ֤সʍށɬɴɪʨᶬಢለኴʆʎ˅˿ʼ̉ʾ̎˜́ʇɶʅ18ŻCrownŻ6ᶨࠏ֤স1.38Ƌᶩʱᦦ૾ɶɾɋ ኚɍ൮ឡʱᨁʌɾᏃೖᶬၑႾႻ҈ʊࡶӁɸʪɲʇʊʧʩ˅˿ʼ̉ʾ̎˜́ʎʸ˿˩ʸˊ˶ʇපʊ ᨅ࢙ᩂʍ৵ᥴɪʃᦋԿʉԎʱԧɸʪɲʇɫሯ៖ɴʫᶬɪʃূಿʍʸ˿˩ʸˊ˶Ⴛ҈ʍܬʧʩ Ⴛ҈ీʱށनʊሀᐗɸʪ՞ೖɫɡʂɾɋʝɾႻ҈ʊ፲ኙ৷ʎʉɮႻ҈াʍ˭̂˧˿̎˞ಢʍ ໑ʡඐʠʅࡄపʆɡʂɾɋ ɉႻ҈ͼʆ˅˿ʼ̉ʾ̎˜́ʎࠏӑʊᩂʺˀ̉ʱכʩ᥈ʲʆҴʣҮԺʉʈʇʍ৵ྟʉטড়ʱ ԧɶʅɣʪʡʍʇᒑɧʨʫᶬॆʎᄴʉʪʡʍʍвᠷˉ̃ʺ˟ʇපʍ՞ೖʱʡɾʨɶᶬᦞฬʉᩂʺ ˀ̉ʇʍ༌๚ʆɡʫʏᶬ᥌ᥴɪʃ֝ԔʉҮɫᢰɲʩᶬвᠷˉ̃ʺ˟ʱᄍɣɾႻ҈ʧʩʡሀీ ʆ֝Ԕʉ൮Ԏও्ʱৃʪɲʇɫʆɬʪʡʍʇ৲ʮʫʪɋಢለኴʍᏃೖʎ˅˿ʼ̉ʾ̎˜́ɫႻ҈ʊ ɩɰʪвᠷˉ̃ʺ˟ʊϐʮʪၑᢑʇɶʅʸ˿˩ʸˊ˶ʧʩҥʫɾោᙪʆɡʪɲʇʱቌɶʅɩʩᶬᮂၔ ᄴᆔטড়ᄊၑʍԎႻɫʉɣautometallographyʱূಿʧʩሀీʊ࠷ఆʆɬʪಐᄍʉႻ҈ʇɶʅໍ
ᄍʆɬʪʡʍʆɡʪɋρাʎᗾᒓɫূಿᄍɣʅɣɾሕ᧦ᩂႻ҈ʊʃɣʅපʊ൮ឡʇᕩʱՒɧᶬ ɴʨʊimmunogoldʱᄍɣɾҴᯑҸᅉ೮ᕫຫᶬ᭙ᯑҸᅉ೮ᕫຫʗʇড়ᄍጳېʱूɱʅʥɮɋ
௮ɉႈ
ᷛ1ᷝ Danscher, G. Histochemical demonstration of heavy metals: a revised version of the sulphide silver method suitable for both light and electron microscopy. (1981) Histochemistry 71, 1Ż16.
ᷛ2ᷝ Danscher, G., Stoltenberg, M. ᶦ Juhl, S. How to detect gold, silver and mercury in human brain and other tissues by autometallographic silver amplification. (1994) Neuropathol. Appl. Neurobiol. 20, 454Ż 67.
ᷛ3ᷝ Fujimori, O., Tsukise, A. and Yamada, K. (1988) Histochemical demonstration of zinc in rat epididymis using a sulphide-silver method. Histochemistry 88, 469Ż473.
ᷛ4ᷝ ᙸ൛ɉиᶮᶨ1992ᶩҴᯑҸᅉ೮ᕫຫᶮීᄑᡤឧᶬᙸ൛ɉиᐁᶮʺ˶ˤˊ̎́˟ຫᶮ˓˫˞ˋʺʾ̉ˏᶬδᶬ pp 49Ż112ᶮ
ᷛ5ᷝ Fujimori, O. (1999) Protein-A-gold/silver staining method: A review of a highly sensitive technique for light microscopic immunohistochemistry. Applied Immunohistochemistry ᶦ Molecular Morphology 7, 280Ż288.
ᷛ6ᷝ Hacker, G. W., Grimelius, L., Danscher, G., Bernatzky, G., Muss, W., Adam, H. ᶦ Thurner, J. (1988) Silver acetate autometallography: An alternative enhancement technique for immunogold-silver staining (IGSS) and silver amplification of gold, silver, mercury and zinc in tissues. J. Histotechnology 11, 213Ż 221.
ᷛ7ᷝ Lüppo-Cramer, H. (1912) Neue Untersuchungen zur Theorie der photographischen Vorgängen. Photographische Korrespond 640, 28Ż34.
ᷛ8ᷝ Pedersen, C. J. (1967) Cyclic polyethers and their complexes with metal salts. J. Am. Chem. Soc. 89, 7017Ż7036.
ᷛ9ᷝ Scopsi, L. (1989) Silver-enhanced colloidal gold method. (ed) Hayat, M. A. in Colloidal gold-Principles, Methos and Applictions. Vol. 1, Academic Press, London, 251Ż295.
1 Faculty of Rehabilitation Sciences
Âãôõóâäõ
Crown ethers are macrocyclic polyethers and these complex with cations and solubilize inorganic reagents into organic solvents. Using a kind of crown ethers, an attempt has been carried out to improve some drawbacks of developing solution including gum Arabic as protective colloid in histochemical silver enhancement techniques. In this study, Crown ether (18ŻcrownŻ6) was introduced to silver acetate developer instead of protective colloid, and the developer improved was applied to sulphide-silver staining method for histochemical detection of zinc in brain and epididymis of rats. Eventually, the present crown ether developer permitted fast and effective development of reaction products and resulted efficient detection sensitivity in aoutometallography.
Ìæúøðóåô: histochemistry, silver enhancement, developer, heavy metal, crown ether
Application of Crown Ether to Silver Enhancement Developer
for Histochemical Autometallography
ɜÓæôæâóäé¡Ïðõæɝ
