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〈平成10∼20年度 研究開発委託事業成果からの展望〉

畜産技術の開発活用促進事業

研究開発の成果と展開方法

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⋡  ᰴ

㧝ࡉ࠲ਥⷐ⚵❱ㆡวⶄว૕/*% 㗔ၞߩㆮવሶ᭴ㅧ⸃ᨆߩㅴዷߣዷᦸ㧔቟⮮㤗ሶ㧕̖̖ 㧞ᩭ⒖ᬀ↪ෆᲣ⚦⢩ߩಓ⚿଻ሽᛛⴚߩㅴዷ㧔⧎↰┨㧕̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ 㧟⇓↥♽ឃ᳓ߩࡔ࠲ࡦ⊒㉂ಣℂᛛⴚߣ⊒㉂ᶧߩ⢈ᢱォ↪ᛛⴚߩዷᦸ㧔ේ↰⑲᮸㧕̖̖̖ 㧠࠙ࠪෆᲣ⚦⢩ߩ૕ᄖᚑᾫၭ㙃ᦼ㑆ߦ₪ᓧߔࠆ⢦⊒↢⢻ߦ෸߷ߔࡒ࠶࠼ࠞࠗࡦߩ   ലᨐߦ㑐ߔࠆ⎇ⓥߩㅴዷߣታ↪ൻ߳ߩዷᦸ㧔ጊ↰㓷଻㧕̖̖̖̖̖̖̖̖̖̖̖̖̖ 㧡㘩ᢱ⥄⛎₸ะ਄ߩߚ߼ߩᨋ㑆᡼’ࠪࠬ࠹ࡓߩౣ᭴▽㧔૒⮮ⴐ੺㧕̖̖̖̖̖̖̖̖̖ 㧢ሶ࠙ࠪߩઍ⻢೙ᓮᛛⴚߩㅴዷߣ໡ຠ㐿⊒㧔ዊේཅᤘ㧕̖̖̖̖̖̖̖̖̖̖̖̖̖̖ 㧣ኅ⇓ឃᴭ‛ߩಣℂ࡮ၸ⢈ൻߦ㑐ࠊࠆᓸ↢‛ߣߘߩᵴ↪㧔ਛ੗⵨㧕̖̖̖̖̖̖̖̖̖ 㧤૕⚦⢩ࠢࡠ࡯ࡦኅ⇓ߩ૞಴ߦ઻߁ㆮવ⊛቟ోᕈߩ⹏ଔ㧔࿖ᨑືᄦ㧕̖̖̖̖̖̖̖̖ 㧥ࡉ࠲⢦ಓ⚿଻ሽߩᛛⴚ㕟ᣂ㧔㐳᎑Ყํᔒ㧕̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ 㘩ຠᱷᷲ߆ࠄࠛࠦࡈࠖ࡯࠼߳㧔㒙ㇱ੫㧕̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ ૕⚦⢩ࠢࡠ࡯ࡦኅ⇓ߦ↱᧪ߔࠆ↢↥‛ߩห╬ᕈ⸃᣿ߩߚ߼ߩ⺖㗴㧔ᷰㄝિ਽㧕̖̖̖ ⇓↥㗔ၞߦ߅ߌࠆ๭ๆ᷹ቯߦࠃࠆ⢦ຠ⾰⹏ଔߩዷᦸ㧔㒙ㇱብਯ㧕̖̖̖̖̖̖̖̖̖ ᛫↢‛⾰ߦઍࠊࠆ⽋↪᛫⩶೷ߩ㐿⊒㧔࿯ደ૫♿㧕̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖ ␹⚻∛ේᕈ⣖Ἳဳ ࠕࠞࡃࡀ࠙ࠗ࡞ࠬߩ∛ේᕈ⸃ᨆߣ⸻ᢿᴺߩ⏕┙㧔᣿⍹ඳ⤿㧕̖̖ 㜞ᴲ੃‐ߦ߅ߌࠆฃ⢝₸ૐਅߩⷐ࿃ߣሶችୃᓳ⹏ଔᴺߩ㐿⊒㧔ዬ࿷ኅ⟵ᤘ㧕̖̖̖̖

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ߪ ߓ ߼ ߦ

 ․଀␠࿅ᴺੱ⇓↥ᛛⴚදળߪޔ⁛┙ⴕ᡽ᴺੱㄘ⇓↥ᬺᝄ⥝ᯏ᭴ߩ⇓↥ᝄ⥝੐ᬺߦࠃࠆ⵬ ഥࠍฃߌߡޔᐔᚑ 10 ᐕᐲ߆ࠄޟ⇓↥ᣂᛛⴚ㐿⊒ᵴ↪ଦㅴ੐ᬺޠ㧔ᐔᚑ 19 ᐕᐲ߆ࠄኅ⇓↢↥ ᣂᛛⴚ᦭ലᵴ↪✚วኻ╷੐ᬺߩਛߩޟ⇓↥ᣂᛛⴚߩ㐿⊒ᵴ↪ଦㅴ੐ᬺޠ㧕ࠍታᣉߒߡ߈߹ߒ ߚޕ  ᧄ੐ᬺߪޔߐ߹ߑ߹ߥ⇓↥ᛛⴚߩ㐿⊒߳ߩⷐᦸߦኻᔕߔࠆߚ߼ޔ⇓↥ో⥸ߦࠊߚࠆ⎇ⓥ ߩផㅴߣߎࠇࠍၮ⋚ߣߒߚᣂᛛⴚߩ㐿⊒߿ᵴ↪ࠍଦㅴߔࠆߎߣࠍ⋡⊛ߣߒޔ10 ᐕ૛ࠅߩ੐ ᬺታᣉᦼ㑆ౝߦᆔ⸤ߒߚ⎇ⓥ㐿⊒⺖㗴ߪ⇓↥ోಽ㊁ߦࠊߚࠅޔᑧߴ 94 ⺖㗴ߦߩ߷ࠅ߹ߔޕ  ߎߩౠሶߢ⚫੺ߔࠆᣂߒ޿⇓↥ᛛⴚߪޔߎߩ੐ᬺߢᆔ⸤ޔታᣉߒߚ⺖㗴ߩਛ߆ࠄޔ⃻࿷ ․ߦ㑐ᔃ߇ᜬߚࠇߡ޿ࠆߣᕁࠊࠇࠆචᢙ⺖㗴ࠍㆬቯߒޔ⎇ⓥࠍᜂᒰߐࠇߚᣇߦ߅㗿޿ߒߡ ߘࠇߙࠇߩ⺖㗴ߦ߅ߌࠆ⎇ⓥᚑᨐࠍၮߦޔߘߩಽ㊁ߩ⎇ⓥ㐿⊒ߩ⃻⁁ޔ੹ᓟߩᣇะ࡮⺖㗴 ╬ߦߟ޿ߡߏၫ╩޿ߚߛ޿ߚ߽ߩߢߔޕ ೎ㅜޔ⎇ⓥࠍᆔ⸤ߒߚ⊝᭽߆ࠄὐᬌޔ⏕⹺޿ߚߛ޿ߚోᆔ⸤⺖㗴ߩᚑᨐࠍขࠅ߹ߣ߼ߚ ੐ᬺో૕ߩႎ๔ᦠߣߣ߽ߦޔ࿖෸߮ㇺ㆏ᐭ⋵ߩ⇓↥㑐ଥ⠪ߦ㈩Ꮣߔࠆ߶߆ޔ⇓↥ᛛⴚදળ ߩᯏ㑐⹹߿ࡎ࡯ࡓࡍ࡯ࠫ╬ߢ߽⚫੺޿ߚߒ߹ߔޕ⎇ⓥ߿ᛛⴚ㐿⊒ޔߘߩᡰេ╬ߦ៤ࠊࠆᣇޘ ߩෳ⠨ߦߥࠇ߫ᐘ޿ߢߔޕ                             ᐔᚑ 20 ᐕ 12 ᦬ ․଀␠࿅ᴺੱ⇓↥ᛛⴚදળ

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ࡉ࠲ਥⷐ⚵❱ㆡวⶄว૕/*% 㗔ၞߩㆮવሶ᭴ㅧ⸃ᨆߩㅴዷߣዷᦸ

㧔᧲ᶏᄢቇකቇㇱ ⻠Ꮷ ቟⮮㤗ሶ㧕 ᐔᚑ 9㨪10 ᐕᐲᆔ⸤ޟኅ⇓ߦ߅ߌࠆਥⷐ⚵❱ㆡวⶄว૕(MHC)㗔ၞߩㆮવሶ᭴ㅧ⸃ᨆޠ

㧔␠࿅ᴺੱㄘᨋ᳓↥వ┵ᛛⴚ↥ᬺᝄ⥝࠮ࡦ࠲࡯ བྷ⸤㧕 㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ

ࡉ࠲ߩਥⷐ⚵❱ㆡว᛫ේⶄว૕(MHC)ߢ޽ࠆ Swine Leukocyte Antigen(SLA)ߪޔߘߩ᛫ ේဳ߇ࡢࠢ࠴ࡦធ⒳ߦࠃࠆ᛫૕↥↢⢻ߥߤߩ఺∉ᔕ╵ᕈ߿ࡔ࡜ࡁ࡯ࡑ⊒∝ߣߩ⋧㑐߇⍮ࠄࠇޔ ⇓↥ቇಽ㊁ߦ߅ߌࠆఝࠇߚ᛫∛ᕈ߿⚻ᷣᒻ⾰ࠍᜬߟࡉ࠲ߩㆬᛯߦ㑐ਈߒߡ޿ࠆߣ⠨߃ࠄࠇߡ ޿ࠆޕ߹ߚޔࡉ࠲ߪ⸃೬ޔ↢ℂቇ⊛ߦࡅ࠻ߣ㘃ૃᕈ߇㜞ߊޔⴊᶧ↢ൻቇ⊛ᢙ୯߽ࡅ࠻ߣㄭ޿ ߎߣ߆ࠄޔࡅ࠻߳ߩ⇣⒳⒖ᬀ࠼࠽࡯ߣߒߡᵈ⋡ߐࠇߡ޿ࠆઁޔ⢈ḩ߿♧ዩ∛ߥߤߩ∔ᖚࡕ࠺ ࡞േ‛ޔක≮ᛛⴚࠪࡒࡘ࡟࡯࡚ࠪࡦߥߤࠍ⋡⊛ߣߒߚታ㛎േ‛ߣߒߡᄙ↪ߐࠇߡ޿ࠆޕߎࠇ ࠄߩ⇓↥ቇಽ㊁ߦ߅ߌࠆఝࠇߚ᛫∛ᕈ߿⚻ᷣᒻ⾰ࠍᜬߟࡉ࠲ߩㆬᛯޔਗ߮ߦකቇޔታ㛎േ‛ ቇಽ㊁ߦ߅ߌࠆห⒳࡮⇣⒳⒖ᬀ⎇ⓥߩផㅴ߿ SLA ࿕ቯ⚐♽ࡒ࠾ࡉ࠲ߩ૞಴ߦ㊀ⷐߥၮ⋚⊛ᖱ ႎߩឭଏޔ߅ࠃ߮᭽ޘߥേ‛⒳㑆ߩ MHC ࠥࡁࡓᖱႎᲧセࠍ⋡⊛ߣߒߡޔSLA 㗔ၞߩࠥࡁࡓ ⸃ᨆߣᄙဳᕈ⸃ᨆࠍⴕߞߚޕ 㧞㧚⎇ⓥ೔㆐ߩ⃻⁁ ࡜࡯ࠫࡎࡢࠗ࠻⒳⽋(Hp-1a.1 ࡂࡊࡠ࠲ࠗࡊ)ߩ SLA ో㗔ၞ(2.4Mb)ߩႮၮ㈩೉⸃⺒ߪޔ 2006 ᐕߦ╩⠪ࠄߩࠣ࡞࡯ ࡊࠍ฽߻ว⸘㧟ࠣ࡞࡯ࡊ ߩ࿖㓙⊛ߥ౒ห⎇ⓥߦࠃ ࠅቢੌߒޔߘߩㆮવሶ᭴ᚑ ߩోኈ߇᣿ࠄ߆ߦߥߞߚ 1)ޕߎߩ㈩೉ᖱႎ߆ࠄޔ SLA 㗔ၞߦߪޔ27 ୘ߩ SLA ㆮ વ ሶ ࠍ ฽ ߻ ว ⸘ 151 ୘ߩㆮવሶ߇หቯߐ ࠇ(࿑ 1)ޔߎߩ㗔ၞߩࡑࠗ ࠢࡠࠨ࠹࡜ࠗ࠻ߩಽᏓߣ ᄙဳᕈ 2)ߥߤߩ․ᓽ߽᣿ ࠄ߆ߦߥࠅޔએਅߦㅀߴࠆ ᛛⴚታ↪ൻ߳ߩၮ⋚ࠍ᭴ ▽ߔࠆߎߣ߇ߢ߈ߚޕ                    ࿑ 1 SLA 㗔ၞߩㆮવሶ᭴ᚑ 㧟㧚ᛛⴚታ↪ൻߩ⃻⁁ ೨㗄ߢㅀߴߚ SLA 㗔ၞߩႮၮ㈩೉ᖱႎߦၮߠ߈ޔ⒳ޘߩຠ⒳ߩ SLA ㆮવሶߩᄙဳ⸃ᨆࠍ ⴕ޿ޔߎࠇ߹ߢߦࠢ࡜ࠬ I ߅ࠃ߮ࠢ࡜ࠬ II ㆮવሶߩ 6 ㆮવሶᐳߦߟ޿ߡޔว⸘ 20 ⒳㘃ߩᣂ ࠕ࡝࡞ࠍ⷗಴ߒߚޕߐࠄߦޔ࿖ౝᄖߢ SLA ㆮવሶߩᄙဳ⸃ᨆ߇ㅴዷߒޔ⃻࿷ 200 ⒳ࠍ⿥߃ࠆ 䋺 ⊒⃻ 㪪㪣㪘㩷䉪䊤䉴 㪠㩷㪃䉪䊤䉴 㪠㩷᭽ㆮવሶ䋺 㪎 䋺 㪪㪣㪘㩷䉪䊤䉴 㪠㩷㪃㩷䉪䊤䉴 㪠㩷᭽னㆮવሶ䋺 㪋 䋺 ⊒⃻ 㪪㪣㪘㩷䉪䊤䉴 㪠㪠㪃㩷䉪䊤䉴 㪠㪠㩷᭽ㆮવሶ䋺 㪏㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷 䋺 㪪㪣㪘㩷䉪䊤䉴 㪠㪠㪃㩷䉪䊤䉴 㪠㪠㩷᭽னㆮવሶ䋺 㪏 䋺 ⊒⃻䇮䉁䈢䈲⊒⃻น⢻ㆮવሶ䋺 㪈㪈㪉 䋺 ᣂ⊒⃻น⢻ㆮવሶ䋺 㪊 䋺 னㆮવሶ䋺 㪐 㪪㪣㪘 ㆮવሶ 㕖㪪㪣㪘 ㆮવሶ ⸘ 㪈㪌㪈 MC C D 1 BA T 1 AT P6 V 1 G2 NF KBI L 1 LT A TN F LT B B X 54 816 9. 9 LS T 1 NC R3 AI F 1 BA T 2 BA T 3 AP OM C7 H 6 o rf 4 7 BA T 4 CN K2 B LY 6G5 B LY 6G5 C BA T 5 LY 6G6 E LY 6G6 D LY 6G6 C C7 H 6 o rf 2 5 DD AH 2 CL IC 1 MS H 5 C7 H 6 o rf 2 6 C7 H 6 o rf 2 7 VA RS 2 LS M 2 HS P A 1 L HS P A 1 A HS P A 1 B AL 7 7 3 5 2 7 .9 NE U1 C7 H 6 o rf 2 BA T 8 ZB T B 1 2 C2 BF RD BP SK IV 2 L DO M3 Z ST K 1 9 C4 A C Y P 2 1A 2 TN X B CR EB L 1 FK B P L C7 H 6 o rf 3 1 PP T2 E G FL8 AG PA T 1 RN F 5 AG ER PB X 2 GP SM 3 NO TC H4 BT NL 5 BT NL 6 ࠮ࡦ࠻ࡠࡔࠕ BT NL4 BT NL3 BT NL2 DR A DR B4 DR B3 DR B2 DR B1 DQ A DQ B2 DQ B1 DO B2 BX 32 38 3 3 .8 BX 32 38 3 3 .9 DR B5 DY B DO B1 TA P 2 PS M B 8 TA P 1 PS M B 9 DM B DM A BR D2 DO A CO L 1 1 A 2 RX RB SL C3 9 A 7 HS D17 B 8 RI NG 1 UB D O R F 4 3-3 O R F 4 3-2 OR F -1 G A BBR1 MO G Zf p5 7 C7 H 6 o rf 1 2 Z N RD1 PP P1 R 1 1 RN F3 9 TR IM 3 1 TR IM 4 0 TR M 1 0 TR M 1 5 TR IM 2 6 AF P RA P1A SL A -1 SL A -5 SL A -9 SL A -3 SL A -2 SL A -4 SL A -11 TR IM 3 9 RP P2 1 GN L 1 PR R3 AB CF1 PP P1 R 1 0 MRP S 1 8 B C7 H 6 o rf 1 34 C7 H 6 o rf 1 36 DH X16 A B 1 1 33 35 6. 6 NR M MDC 1 T U BB FL OT 1 IE R 3 A B 1 1 33 5. 2 DD R1 GT F 2 H 4 VA RS2 L A B 1 1 33 54 .6 DP CR 1 C7 H 6 o rf 2 05 C7 H 6 o rf 1 5 A B 1 1 13 35 4. 10 PS OR S 1 C 1 PS OR S 1 C 2 CC HC R1 TC F 1 9 PO U5F 1 MI C 2 MI C 1 SL A -8 SL A -7 SL A -6 ࠹ ࡠ ࡔ ࠕ ࠢ࡜ࠬ+Dㆮવሶ⟲ ࠢ࡜ࠬ+㗔ၞ ࠢ࡜ࠬ+Cㆮવሶ⟲ ࠢ࡜ࠬ+++㗔ၞ ࠢ࡜ࠬ++㗔ၞ િ㐳ࠢ࡜ࠬ++㗔ၞ િ㐳ࠢ࡜ࠬ+㗔ၞ ࠹ ࡠ ࡔ ࠕ 䋺 ⊒⃻ 㪪㪣㪘㩷䉪䊤䉴 㪠㩷㪃䉪䊤䉴 㪠㩷᭽ㆮવሶ䋺 㪎 䋺 㪪㪣㪘㩷䉪䊤䉴 㪠㩷㪃㩷䉪䊤䉴 㪠㩷᭽னㆮવሶ䋺 㪋 䋺 ⊒⃻ 㪪㪣㪘㩷䉪䊤䉴 㪠㪠㪃㩷䉪䊤䉴 㪠㪠㩷᭽ㆮવሶ䋺 㪏㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷 䋺 㪪㪣㪘㩷䉪䊤䉴 㪠㪠㪃㩷䉪䊤䉴 㪠㪠㩷᭽னㆮવሶ䋺 㪏 䋺 ⊒⃻䇮䉁䈢䈲⊒⃻น⢻ㆮવሶ䋺 㪈㪈㪉 䋺 ᣂ⊒⃻น⢻ㆮવሶ䋺 㪊 䋺 னㆮવሶ䋺 㪐 㪪㪣㪘 ㆮવሶ 㕖㪪㪣㪘 ㆮવሶ ⸘ 㪈㪌㪈 MC C D 1 BA T 1 AT P6 V 1 G2 NF KBI L 1 LT A TN F LT B B X 54 816 9. 9 LS T 1 NC R3 AI F 1 BA T 2 BA T 3 AP OM C7 H 6 o rf 4 7 BA T 4 CN K2 B LY 6G5 B LY 6G5 C BA T 5 LY 6G6 E LY 6G6 D LY 6G6 C C7 H 6 o rf 2 5 DD AH 2 CL IC 1 MS H 5 C7 H 6 o rf 2 6 C7 H 6 o rf 2 7 VA RS 2 LS M 2 HS P A 1 L HS P A 1 A HS P A 1 B AL 7 7 3 5 2 7 .9 NE U1 C7 H 6 o rf 2 BA T 8 ZB T B 1 2 C2 BF RD BP SK IV 2 L DO M3 Z ST K 1 9 C4 A C Y P 2 1A 2 TN X B CR EB L 1 FK B P L C7 H 6 o rf 3 1 PP T2 E G FL8 AG PA T 1 RN F 5 AG ER PB X 2 GP SM 3 NO TC H4 BT NL 5 BT NL 6 ࠮ࡦ࠻ࡠࡔࠕ BT NL4 BT NL3 BT NL2 DR A DR B4 DR B3 DR B2 DR B1 DQ A DQ B2 DQ B1 DO B2 BX 32 38 3 3 .8 BX 32 38 3 3 .9 DR B5 DY B DO B1 TA P 2 PS M B 8 TA P 1 PS M B 9 DM B DM A BR D2 DO A CO L 1 1 A 2 RX RB SL C3 9 A 7 HS D17 B 8 RI NG 1 UB D O R F 4 3-3 O R F 4 3-2 OR F -1 G A BBR1 MO G Zf p5 7 C7 H 6 o rf 1 2 Z N RD1 PP P1 R 1 1 RN F3 9 TR IM 3 1 TR IM 4 0 TR M 1 0 TR M 1 5 TR IM 2 6 AF P RA P1A SL A -1 SL A -5 SL A -9 SL A -3 SL A -2 SL A -4 SL A -11 TR IM 3 9 RP P2 1 GN L 1 PR R3 AB CF1 PP P1 R 1 0 MRP S 1 8 B C7 H 6 o rf 1 34 C7 H 6 o rf 1 36 DH X16 A B 1 1 33 35 6. 6 NR M MDC 1 T U BB FL OT 1 IE R 3 A B 1 1 33 5. 2 DD R1 GT F 2 H 4 VA RS2 L A B 1 1 33 54 .6 DP CR 1 C7 H 6 o rf 2 05 C7 H 6 o rf 1 5 A B 1 1 13 35 4. 10 PS OR S 1 C 1 PS OR S 1 C 2 CC HC R1 TC F 1 9 PO U5F 1 MI C 2 MI C 1 SL A -8 SL A -7 SL A -6 ࠹ ࡠ ࡔ ࠕ ࠢ࡜ࠬ+Dㆮવሶ⟲ ࠢ࡜ࠬ+㗔ၞ ࠢ࡜ࠬ+Cㆮવሶ⟲ ࠢ࡜ࠬ+++㗔ၞ ࠢ࡜ࠬ++㗔ၞ િ㐳ࠢ࡜ࠬ++㗔ၞ િ㐳ࠢ࡜ࠬ+㗔ၞ ࠹ ࡠ ࡔ ࠕ

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ࠕ࡝࡞߿ࡂࡊࡠ࠲ࠗࡊߩฬ⒓߇╩ ⠪ࠍ฽߻ 6 ฬߩᆔຬ߆ࠄ᭴ᚑߐࠇ ࠆ ISAG SLA Nomenclature

Committee ߦࠃࠅ೙ቯߐࠇޔߎࠇ ࠄߩᖱႎߪޔ᭽ޘߥಽ㊁ߩ MHC ⎇ ⓥߦ⽸₂ߒߡ޿ࠆ(http://www.ebi. ac.uk /ipd/mhc/sla/index.html㧕ޕ ߹ߚޔSLA 㗔ၞߩࠥࡁࡓ߿ SLA cDNA ࠢࡠ࡯ࡦߩႮၮ㈩೉ᖱႎߦ ၮ ߠ ޿ ߡ 㐿 ⊒ ߒ ߚ ◲ ଢ ߥ SLA-DNA ࠲ࠗࡇࡦࠣᴺࠍ↪޿ߡޔ ߎ ࠇ ߹ ߢ ᶏ ᄖ ߢ ႎ ๔ ߐ ࠇ ߡ ޿ ࠆ NIHࡒ࠾ࡉ࠲ߥߤߩ SLA ࿕ቯࡉ࠲ߣߪ⇣ߥࠆᣂࡂࡊࡠ࠲ࠗࡊࠍᜬߟ 2 ຠ⒳(ࠢ࡜࠙ࡦࡒ࠾ࡉ ࠲ޔ࠺ࡘࡠ࠶ࠢ⒳ࡉ࠲3))ߩࡉ࠲ࠍ૞಴ߒߚ(⴫ 1)ޕߎࠇࠄߩ SLA ࿕ቯࡉ࠲ߩਛߢޔࠢ࡜࠙ࡦ ࡒ࠾ࡉ࠲ߦߟ޿ߡߪޔ(ᩣ)ࠫࡖࡄࡦࡈࠔ࡯ࡓࠃࠅޔSLA ࿕ቯࡉ࠲ߣߒߡ⽼ᄁଏ⛎ߐࠇޔකᎿ ቇ↪ࠍೋ߼ߣߔࠆ᭽ޘߥಽ㊁ߢታ㛎േ‛ߣߒߡᵴ↪ߐࠇߡ޿ࠆޕ 㧠㧚੹ᓟߩᣇะޔ⺖㗴  ߎࠇ߹ߢߦ⸃ᨆߒߚ SLA 㗔ၞߩࠥࡁࡓ㈩೉ߣࡑࠗࠢࡠࠨ࠹࡜ࠗ࠻ࡑ࡯ࠞ࡯߿ SLA ㆮવሶ ߩᄙဳᕈᖱႎߪޔ2009 ᐕߦ⸃⺒ቢੌ੍ቯߩࡉ࠲ోࠥࡁࡓ㈩೉ᖱႎߣวࠊߖߡޔ∔ᖚᗵฃᕈ߿ ᛶ᛫ᕈߦ㑐ਈߔࠆ఺∉㑐ㅪㆮવሶࠍ฽߻᭽ޘߥㆮવሶߩ✂⟜⊛ߥᄙဳ࡮⊒⃻⸃ᨆ߳⊒ዷߒޔ ฦ⒳∔ᖚᗵฃᕈߦ㑐ࠊࠆᯏ᭴⸃᣿߳ߣ⎇ⓥ߇ㅴዷߔࠆߣ⠨߃ࠄࠇࠆޕ ৻ᣇޔ╩⠪ࠄ߇㐿⊒ߒߚ 2 ຠ⒳ߩ SLA ࿕ቯࡉ࠲ߪޔห⒳߅ࠃ߮⇣⒳⤳ེ⒖ᬀታ㛎ߦࠃࠆ MHC ㆡวᐲߣ੍ᓟߣߩ㑐ㅪᕈ⸃ᨆߩઁޔ఺∉㑐ㅪ⮎೷ߩ㐿⊒ޔᛩ⮎ࠪࡒࡘ࡟࡯࡚ࠪࡦ߿∔ ᖚࡕ࠺࡞߅ࠃ߮ౣ↢ක≮ߥߤߩක≮ಽ㊁ߦ߅޿ߡޔታ㛎േ‛ߣߒߡߩ࿖ౝᄖߩ⎇ⓥ೑↪ߩჇ ᄢ߇ᦼᓙߐࠇࠆޕ߹ߚޔ᛫∛ᕈຠ⒳ߩㆬᛯࡑ࡯ࠞ࡯ߣߒߡߩ SLA ㆮવሶߩ᦭↪ᕈߩ⸃᣿ߩߚ ߼ߦޔSLA ࿕ቯࡉ࠲ࠍ↪޿ߚޔࡢࠢ࠴ࡦធ⒳ߥߤߩฦ⒳ᄖ᧪᛫ේߦኻߔࠆ఺∉ᔕ╵ᕈߣ SLA ࠲ࠗࡊߣߩ⋧㑐⸃ᨆ߇ㅴⴕਛߢ޽ࠆޕߎߩࠃ߁ߥ⎇ⓥߦട߃ߡޔวᚑࡍࡊ࠴࠼ߥߤߩ⒳ޘߩ ∔ᖚ㑐ㅪ᛫ේߣߩ㑐ㅪᕈࠍ⸃ᨆߔࠆߎߣߦࠃࠅޔᗵᨴ∝ߦኻߔࠆ∔ᖚᗵฃᕈ߿ᛶ᛫ᕈ߅ࠃ߮ ࡢࠢ࠴ࡦ㐿⊒ޔߐࠄߦ⚻ᷣᒻ⾰ߣߩ㑐ㅪᕈߦߟ޿ߡ߽᦭↪ߥᖱႎ߇ᓧࠄࠇࠆޕએ਄ߩࠃ߁ߦޔ SLA࿕ቯࡉ࠲ߪޔ᭽ޘߥಽ㊁ߩࡃࠗࠝ࡝࠰࡯ࠬߣߒߡᭂ߼ߡ㊀ⷐߢ޽ࠆߣ⠨߃ࠄࠇޔᣂߚߥ ⇓↥㑐ㅪ↥ᬺߩ㐿ᜏߦ❬ߍࠆߚ߼ߦ߽ޔSLA ࿕ቯࡉ࠲ߩ♽⛔⛽ᜬߣታ㛎േ‛ߣߒߡߩଏ⛎ޔ ߅ࠃ߮᭽ޘߥᛛⴚ㐿⊒⾗㊄ߩᡰេ߇ಾߦᦼᓙߐࠇࠆޕ 㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥ౏⴫ႎ๔╬

㧝㧕Renard C., et al.㧦Genomics 88, 96-110, 2006.

㧞㧕Ando A., et al.㧦 Immunogenetics 60, 399-407, 2008. 㧟㧕Soe OK., et al.㧦 Xenotransplantation, In press.

ຠ⒳

(ᣥฬ⒓) SLA-1 SLA-2 SLA-3 DRB1 DQB1

Clawn 16.16 (c1) *0401 *w09an02 *0602 *11ac21 *0601 17.17 (c2) null? *06an03 *03an02 *0801 *0501

16.17(c3) *0401 *w09an02 *0602 *0801 *0501

17.16(c4) null? *06an03 *03an02 *11ac21 *0601

Duroc 27.30 (d1) *08an03, *06an04 *0102 *0101 *1101 *0503

60.13 (d2) *-an02 *1002 *0502 *0403 *0303

Large White 1.1 (H01) *0101 *0101 *0101 *0101 *0101

NIH (MGH) 2.2 (a) *0201, *0701 *0201 null *0201 *0201

3.3 (c) null *0301 *0301 *0301 *0301

4a.4 (d) *0401 *0401 *0401 *0201 *040101

Yucatan 4b.5(x) *0401 *040201 *0401 *0501 *0201

5.6 (w) *0401 *w08sw01 *05sw01 *0501 *0801

6.7 (y) *08sy01 *05sy01 *0601 *0601 *0601

7.8 (z) *0801 *0502 *0701 *0801 *0202

Westran 8.9 *02we02, *04we01 *07we01 *0302 *0201 *0402we01

Sinclair 9.10 (a) *0601 *0601 *0501 *0401 *0801 10.11 (c) *0501 *0302 *hm22 *0901 *0402 11.12 (d) *0101, *w09sm09 *0501 *0701sm19 *0602 *0701 Hanford 12.13 (e) *08sm08, *w09sm09 *10sm01 *0701sm19 *0403 *0303 13.0 (f) *w10sm21 *w13sm20 *0401 NT NT ⴫ 㪈㩷૞಴䈚䈢䉪䊤䉡䊮䊚䊆䊑䉺䇮䊂䊠䊨䉾䉪⒳䊑䉺䇮䈍䉋䈶ႎ๔䈘䉏䈩䈇䉎㪪㪣㪘࿕ቯ䊑䉺䈱䊊䊒䊨䉺䉟䊒 ੤Ꮕ䊊䊒䊨䉺䉟䊒 䉪䊤䉴 I ㆮવሶ 䉪䊤䉴 II ㆮવሶ 䊊䊒䊨䉺䉟䊒: Hp-ຠ⒳

(ᣥฬ⒓) SLA-1 SLA-2 SLA-3 DRB1 DQB1

Clawn 16.16 (c1) *0401 *w09an02 *0602 *11ac21 *0601 17.17 (c2) null? *06an03 *03an02 *0801 *0501

16.17(c3) *0401 *w09an02 *0602 *0801 *0501

17.16(c4) null? *06an03 *03an02 *11ac21 *0601

Duroc 27.30 (d1) *08an03, *06an04 *0102 *0101 *1101 *0503

60.13 (d2) *-an02 *1002 *0502 *0403 *0303

Large White 1.1 (H01) *0101 *0101 *0101 *0101 *0101

NIH (MGH) 2.2 (a) *0201, *0701 *0201 null *0201 *0201

3.3 (c) null *0301 *0301 *0301 *0301

4a.4 (d) *0401 *0401 *0401 *0201 *040101

Yucatan 4b.5(x) *0401 *040201 *0401 *0501 *0201

5.6 (w) *0401 *w08sw01 *05sw01 *0501 *0801

6.7 (y) *08sy01 *05sy01 *0601 *0601 *0601

7.8 (z) *0801 *0502 *0701 *0801 *0202

Westran 8.9 *02we02, *04we01 *07we01 *0302 *0201 *0402we01

Sinclair 9.10 (a) *0601 *0601 *0501 *0401 *0801 10.11 (c) *0501 *0302 *hm22 *0901 *0402 11.12 (d) *0101, *w09sm09 *0501 *0701sm19 *0602 *0701 Hanford 12.13 (e) *08sm08, *w09sm09 *10sm01 *0701sm19 *0403 *0303 13.0 (f) *w10sm21 *w13sm20 *0401 NT NT ⴫ 㪈㩷૞಴䈚䈢䉪䊤䉡䊮䊚䊆䊑䉺䇮䊂䊠䊨䉾䉪⒳䊑䉺䇮䈍䉋䈶ႎ๔䈘䉏䈩䈇䉎㪪㪣㪘࿕ቯ䊑䉺䈱䊊䊒䊨䉺䉟䊒 ੤Ꮕ䊊䊒䊨䉺䉟䊒 䉪䊤䉴 I ㆮવሶ 䉪䊤䉴 II ㆮવሶ 䊊䊒䊨䉺䉟䊒:

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Hp-ᩭ⒖ᬀ↪ෆᲣ⚦⢩ߩಓ⚿଻ሽᛛⴚߩㅴዷ

㧔␠࿅ᴺੱ⇓↥ᛛⴚදળ ෳਈ ⧎↰ ┨㧕 ᐔᚑ 9㨪10 ᐕᐲᆔ⸤ޟᩭ⒖ᬀߦ↪޿ࠆ࡟ࠪࡇࠛࡦ࠻ෆᲣ⚦⢩ߩಓ⚿ᛛⴚ㐿⊒ޠ㧔ାᎺᄢቇ ᢎ᝼㧕 㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ  ࠢࡠ࡯ࡦ૞಴(ᩭ⒖ᬀ)ᛛⴚߪ㒰ᩭಣℂߣᵴᕈൻಣℂࠍᣉߒߚᚑᾫෆᲣ⚦⢩ߩ⺞⵾ࠍᔅⷐߣ ߒޔߎߩ࡟ࠪࡇࠛࡦ࠻ෆᲣ⚦⢩ߣ࠼࠽࡯⚦⢩ߣࠍ㔚᳇⊛ߦⲢวߐߖߚౣ᭴▽⢦ߩ⒖ᬀߦࠃߞ ߡޔࠢࡠ࡯ࡦ୘૕ࠍ૞಴ߔࠆޕߘߩ㓙ޔ࡟ࠪࡇࠛࡦ࠻ෆᲣ⚦⢩ߩಓ⚿଻ሽ߇น⢻ߦߥࠇ߫ޔ ࠼࠽࡯⚦⢩ߩ೑↪ᕈ߇ะ਄ߒޔࠢࡠ࡯ࡦ૞಴ᛛⴚߩ᥉෸ߦ৻ጀᜉゞ߇߆߆ࠆ߽ߩߣᦼᓙߐࠇ ࠆޕᧄ⎇ⓥߢߪ࠙ࠪߩᩭ⒖ᬀߦ↪޿ࠆ࡟ࠪࡇࠛࡦ࠻ෆᲣ⚦⢩ߩಓ⚿଻ሽᛛⴚࠍ⏕┙ߔࠆߎߣ ࠍ⋡⊛ߦޔ㧝㧕⵻ൻෆᲣ⚦⢩ߦㆡߒߚಓ⚿Ⲣ⸃᧦ઙࠍᒻᘒᱜᏱᕈߦၮߠ޿ߡ᳿ቯߒޔᰴ޿ߢ 㧞㧕ಓ⚿ಣℂߩᤨᦼࠍᦨㆡൻߔࠆߚ߼ߩᩭ⒖ᬀታ㛎ࠍⴕߞߚޕ  ታ㛎㧝㧕ੑᲑ㓏ಓ⚿ᴺߣࠟ࡜ࠬൻ଻ሽᴺߩ޿ߕࠇߩ⿥ૐ᷷଻ሽᣇᴺߦ߅޿ߡ߽ޔࠛ࠴࡟ࡦ ࠣ࡝ࠦ࡯࡞ߩಓኂ଻⼔ലᨐ߇߽ߞߣ߽ఝࠇߡ߅ࠅޔࠪࡘࠢࡠ࡯ࠬߩᷝടߪߐࠄߦ᦭ലߥࠃ߁ ߢ޽ߞߚޕ߹ߚޔෆᲣ⚦⢩ߩⲢ⸃ᓟߩᒻᘒᱜᏱ₸ߪࠟ࡜ࠬൻ଻ሽᴺࠃࠅ߽ੑᲑ㓏ಓ⚿ᴺߩ߶ ߁߇㜞߆ߞߚޕ  ታ㛎㧞㧕ೋᦼಽഀߪߔߴߡߩಓ⚿⟲ߢⷰኤߐࠇ(38㨪73%)ޔ⊒↢ㅦᐲߣ᭴ᚑ⚦⢩ᢙߦ㑐ߒߡ ኻᾖ⟲ߣᏅߩߥ޿⢦⋚⢩߇ᓧࠄࠇߚޕߒ߆ߒޔ⊒↢₸ߪࠢࡠ࡯ࡦฃ♖ෆᒰߚࠅߢ 2㨪6%ޔኻ ᾖ⟲ߩ୯߆ࠄߺࠆߣ 5㨪14%ߦㆊ߉ߥ߆ߞߚޕ  ⚿⺰ߣߒߡޔ㒰ᩭᓟߩ࠙ࠪෆᲣ⚦⢩ࠍࠛ࠴࡟ࡦࠣ࡝ࠦ࡯࡞ߣࠪࡘࠢࡠ࡯ࠬߩਔᣇࠍ଻⼔‛ ⾰ߣߒߡੑᲑ㓏ಓ⚿ߒߡ߆ࠄᩭ⒖ᬀߔࠆߎߣߦࠃࠅޔ⢦⋚⢩߳ߩ⊒↢଀߇ᓧࠄࠇࠆߎߣޔ߹ ߚޔߘߩಓ⚿଻ሽߩ࠲ࠗࡒࡦࠣߪࠢࡠ࡯ࡦ⢦ߩ૞಴ല₸ߦᓇ㗀ࠍ෸߷ߔⷐ࿃ߩ߭ߣߟߢ޽ࠆ ߎߣ߇᣿ࠄ߆ߦߥߞߚޕ 㧞㧚⎇ⓥ೔㆐ߩ⃻⁁  ಓ⚿଻ሽߒߚ࠙ࠪෆᲣ⚦⢩ߦᩭ⒖ᬀࠍᣉߒߡ߽ޔฃ⢦‐ߦ⒖ᬀน⢻ߥ⢦⋚⢩ᦼ߳ߩ⊒↢₸ ߪ㕖Ᏹߦૐ޿ߣႎ๔ߐࠇߡ߅ࠅޔᧄ⎇ⓥߢ߽ಓ⚿Ⲣ⸃ߩᓇ㗀ߪ⢦⋚⢩߳ߩ⊒↢ߩㆊ⒟ߢ߽ߞ ߣ߽㗼⪺ߦ⃻ࠇߚޕ⎇ⓥታᣉᒰᤨޔෆᲣ⚦⢩ߩಓ⚿଻ሽߪ࿎㔍ߣ⠨߃ࠄࠇߡ߅ࠅޔߘߩὐࠍ ⠨ᘦߔࠇ߫వ㚟⊛⎇ⓥߢߪ޽ߞߚ߇ޔᓧࠄࠇߚ⚿ᨐߩ߹߹ߢߪ⇓↥⃻႐ߦᵴ↪ߢ߈ࠆߣߪ޿ ߃ߥ߆ߞߚޕ  ߒ߆ߒޔᧄ⎇ⓥߪᒰ⹥ಽ㊁ߩ⎇ⓥ⠪ࠍೝỗߒޔߘߩᓟߩૐ᷷↢‛ቇߣಓ⚿଻ሽᛛⴚߩᔀᐩ ⊛ߥㅴዷߦࠃߞߡᛛⴚߩ⏕┙ࠍ⷗ࠆߦ⥋ߞߚޕߎࠇߪ⿥ᕆㅦࠟ࡜ࠬൻ଻ሽᴺߣ޿ࠊࠇࠆ߽ߩ ߢޔᓥ᧪ߩࠟ࡜ࠬൻ଻ሽᴺߩ಄ළ́Ⲣ⸃ㅦᐲࠍᡷༀߒߚᣇᴺߢ޽ࠆޕࠟ࡜ࠬൻ଻ሽߣߪޔ㜞 Ớᐲߩಓ⚿଻⼔‛⾰ࠍᷝടߒߚṁᶧߦࠃߞߡ⹜ᢱ⚦⢩ࠍචಽ⣕᳓ߒޔ⚦⢩ౝᄖߢߩ᳖᥏ᒻᚑ ࠍ㒐߉ޔࠟ࡜ࠬൻ⁁ᘒߢᶧ૕⓸⚛ਛߦ଻ሽߔࠆᣇᴺߢ޽ࠆޕᧄ⎇ⓥߣห᭽ߦޔಓ⚿଻⼔‛⾰ ߦߪ⚦⢩߳ߩㅘㆊᕈ߇㜞ߊޔᲥᕈ߇ዋߥ޿ࠛ࠴࡟ࡦࠣ࡝ࠦ࡯࡞ߣ⣕᳓ଦㅴߩߚ߼ߩࠪࡘࠢࡠ ࡯ࠬ(㕖ㅘㆊᕈ)ࠍ⚵ߺวࠊߖߚṁᶧࠍ૶↪ߔࠆޕ  ໧㗴ߪෆᲣ⚦⢩ߩኈེߢޔᧄ⎇ⓥߢߪࠬ࠻ࡠ࡯ࠍ૶↪ߒߚ߇ޔን᳗ࠄ(2001)ߪᏒ⽼ߩ࠴࠶ ࡊࠍᵹ↪ߒߚࠥ࡞ࡠ࡯࠺ࠖࡦࠣ࠴࠶ࡊᴺࠍ㐿⊒ߒߚ(౮⌀)ޕ  ߘߩ೑ὐߪࠝ࡯ࡊࡦߥኈེߩߚ߼ಓ⚿ᤨߩ‛ℂ⊛࿶ജࠍ㒐߉ޔߏߊዋ㊂ߩᶧ(0.6ǴͰ)ߣ౒ߦ

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ෆᲣ⚦⢩ࠍ⿥ᕆㅦ಄ළߔࠆߎߣߦ޽ࠆޕห᳁ࠄ(2005)ߪߎߩ଻ሽෆᲣ⚦⢩߇ޔⲢ⸃ᓟޔ95% ߇ᒻᘒ⊛ߦᱜᏱߢޔ㒰ᩭ₸ 88%ޔᝌ౉ߒߚෆਐ⚦⢩ߣߩⲢว₸ 79%ޔෆഀ₸ 70%ޔ⢦⋚⢩⊒ ↢₸ 45%ߣ޿߁㜞޿ᚑഞ₸ࠍႎ๔ߒߚޕ 㧟㧚ᛛⴚታ↪ൻߩ⃻⁁  ਄ㅀߩࠃ߁ߦᧄᛛⴚߪߔߢߦቢᚑߒߡ߅ࠅޔ࠙ࠪߩߺߥࠄߕૐ᷷ᗵฃᕈߩ㜞޿ࡉ࠲ࠍ฽߼ ߚઁߩູ੃㘃ߩෆᲣ⚦⢩߿⢦ߩ଻ሽߦ߽᦭ലߢ޽ࠆޕࡅ࠻ෆᲣ⚦⢩ߢߪ⚂ 100%ߩᚑഞ₸ߢ ޽ࠅޔ㗼ᓸ᝼♖ߣߩ⚵ߺวࠊߖߦࠃࠆਇᅧᴦ≮ߦᔅ㗇ߩᛛⴚߣߒߡ਎⇇⊛ߦᵴ↪ߐࠇߡ޿ࠆ (᪀ጊߩࠢ࡜ࠗࠝ࠻࠶ࡊᴺޔ2005)ޕ 㧠㧚੹ᓟߩᣇะޔ⺖㗴  㐿⊒⾗㊄߳ߩᡰេⷐ⺧ߪᄙ޿ޕߘߩ႐วޔ⎇ⓥ⺖㗴ౝኈߩክᩏߩߺߥࠄߕޔ᳿ቯᓟߩ⎇ⓥ ㅴ᝞⁁ᴫߣ⚳ੌᓟߩㅴዷ⁁ᴫߩᛠី߇ࠃࠅ㊀ⷐߢ޽ࠆޕ 㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥ౏⴫ႎ๔╬

㧝㧕Tominaga K., Hamada Y., Hochi S.㧦Gel-loading tip vitrification of in vitro-matured bovine oocytes and subsequent embryo production by IVF and nuclear transfer. J.Mamm.Ova Res.22, 178-184, 2005.

㧞㧕Kuwayama M.㧦Oocyte cryopreservation.( Mini Review) J.Mamm.Ova Res.24,2-7, 2007.

౮⌀㧦(਄) ࠥ࡞ࡠ࡯࠺ࠖࡦࠣ࠴࠶ࡊ㧦ෆᲣ⚦⢩ߣಓ⚿ᶧࠍๆᒁߒޔ ᶧ૕⓸⚛ਛߦᶐߔޕ

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⇓↥♽ឃ᳓ߩࡔ࠲ࡦ⊒㉂ಣℂᛛⴚߣ⊒㉂ᶧߩ⢈ᢱォ↪ᛛⴚߩዷᦸ

㧔᧲ർᄢቇᄢቇ㒮Ꮏቇ⎇ⓥ⑼ ᢎ᝼ ේ↰⑲᮸㧕 ᐔᚑ 10㨪11 ᐕᐲᆔ⸤ޟᄙᲑᑼ UASB ࡊࡠ࠮ࠬߦࠃࠆ⇓↥ឃ᳓ߩ㜞᷷ࡔ࠲ࡦ⊒㉂ಣℂߣ MAP ᨆ಴ᴺߦࠃࠆⴡ ↢ቇ⊛ߦ቟ోߥ⢈ᢱ↢↥ᛛⴚߩ⏕┙ޠ㧔㐳ጟᛛⴚ⑼ቇᄢቇ ᢎ᝼㧕 㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ ⇓↥ᑄ᫈‛ߪޔ⾆㊀ߥ᦭ᯏ⾰⾗Ḯߣߒߡޔߎࠇ߹ߢㄘ↥‛߿㘺ᢱ૞‛ߩ↢↥ߦ᦭ലߦ೑↪ ߐࠇߡ߈ߚ߇ޔ⇓↥⚻༡ߦ߅ߌࠆᕆㅦߥⷙᮨ᜛ᄢ╬ߦࠃࠅឃߖߟ‛ߩ⊒↢㊂߇Ⴧᄢߒޔ⥄Ꮖ ⚻༡ౝ޽ࠆ޿ߪ࿾ၞౝߦ߅ߌࠆ᦭ല೑↪߇࿎㔍ߦߥߞߡ߈ߡ޿ࠆޕߐࠄߦኅ⇓ឃߖߟ‛ᴺߩ ቢోᣉⴕߦࠃࠅޔߘߩಣℂߪ߽ߣࠃࠅޔㆡᱜߥ▤ℂ࡮೑↪ߩଦㅴ߇ᒝߊ᳞߼ࠄࠇߡ޿ࠆޕ ߘߎߢᧄ⎇ⓥߢߪޔ⇓↥♽ឃ᳓ߩല₸⊛ߥಣℂߣ㜞ല₸ࠛࡀ࡞ࠡ࡯࿁෼ࠍ⋡⊛ߣߒߡޔᧄ ⎇ⓥቶߢ㐿⊒ߒߚᄙᲑᑼ UASB ࡊࡠ࠮ࠬࠍㆡ↪ߒޔߘߩ᦭ലᕈߦߟ޿ߡᬌ⸛ߒߚޕ߹ߚ⇓↥ ♽ឃ᳓߆ࠄߩ⢈ᢱᚑಽ(⓸⚛㨯࡝ࡦ)ߩ࿁෼ࠍ⹜ߺࠆߚ߼ߦޔ⽋⥢ឃ᳓ߩ UASB ಣℂ᳓ࠍ↪޿ߡޔ MAPᨆ಴ᴺߩ⻉᧦ઙߦߟ޿ߡߩၮ␆⊛ᬌ⸛ࠍⴕߞߚޕ 㧞㧚⎇ⓥ೔㆐ߩ⃻⁁ 㧝㧕ᄙᲑဳ UASB ࡝ࠕࠢ࠲࡯ߩㅪ ⛯ಣℂታ㛎㧦  ࿑㧝ߦ␜ߔᄙᲑᑼ UASB ࠍ↪޿ޔ CODỚᐲࠍ⚂ 21,600mg-COD/lޔSS Ớᐲࠍ⚂ 12,800mg/l ߦ⺞ᢛߒߚឃ᳓ ࠍޔᓴⅣᲧ 10㨪20 ߢಣℂߒߚ⚿ᨐޔ ኈ Ⓧ ⽶ ⩄ 4.0 㨪 5.4 kg-COD m-3 d-1(HRT ⚂ 5 ᣣ)ߢޔో COD ߅ࠃ߮ ṁ⸃ᕈ㒰෰₸ߣ߽ߦ 85%એ਄ޔో VFAỚᐲ 250mg-COD/l એਅޔࡔ࠲ࡦ ォ឵₸ 70㧑⒟ᐲߢㆇォน⢻ߢ޽ߞߚޕ 㧞㧕ህ᳇ᕈಣℂ᳓ߦ߅ߌࠆ MAP ↢ ᚑ᧦ઙ╬ߩᬌ⸛㧦 Mg/Pࡕ࡞Ყࠍ 1.3 ߦ⸳ቯߒ pH8㨪9 ߩ▸࿐ߢህ᳇ᕈಣℂ᳓ߩ MAP ᨆ಴ታ 㛎ࠍⴕߞߚ⚿ᨐࠍ࿑㧞ߦ␜ߔޕ࡝ࡦߦ 㑐ߒߡߪޔpH10 ߦ߅޿ߡᦨ߽ఝࠇߚ 㒰෰₸߇␜ߐࠇߚ߇ޔࠕࡦࡕ࠾ࠕߩ㒰 ෰₸ߪ 20%⒟ᐲߢ޽ߞߚޕ↢ᚑߐࠇߚ ⚿᥏ߦߟ޿ߡ EDX ߢቯᕈಽᨆࠍⴕߞ ߚߣߎࠈޔP ߣ Mg એᄖߦ Ca ߩሽ࿷ ߇⏕⹺ߐࠇߚޕ߹ߚޔ⚿᥏ߩ X ✢࿁᛬

ߦࠃࠅޔMAP એᄖߦࡅ࠼ࡠࠠࠪࠕࡄࠨࠗ࠻(HAP)ߩሽ࿷߇⏕⹺ߢ߈ߚޕHAP ߩ↢ᚑߪ MAP ෻ᔕᤨߦࠕࡦࡕ࠾ࠕ㒰෰ࠍ㒖ኂߔࠆߎߣ߆ࠄޔ੹ᓟߩኻ╷߇ᔅⷐߢ޽ࠆߣ⠨߃ࠄࠇߚޕߥ߅ޔ SS߇฽߹ࠇߡ޿ߡ߽࡝ࡦߩ㒰෰₸ߦᄢ߈ߥᄌൻߪߺࠄࠇߥ߆ߞߚޕ ࿑㧝 ᄙᲑᑼ UASB ࡝ࠕࠢ࠲࡯ 0 20 40 60 80 100 7 8 9 10 11 pH R em ova l(% ) PO4-P PO4-P(SS) NH4-N NH4-N(SS) ࿑㧞 MAP ᨆ಴ᴺߩ⚿ᨐ

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㧟㧚ᛛⴚታ↪ൻߩ⃻⁁ ⃻࿷ޔ⇓↥ឃ᳓ߩಣℂᣇᴺߣߒߡ UASB ᴺ߇ᵈ⋡ࠍᶎ߮ޔߘߩㆡ↪੐଀߽Ⴧ߃ߟߟ޽ࠆޕ ߒ߆ߒߥ߇ࠄޔ޿߹ߛ෻ᔕᮏౝߢߩ SS ߩ⫾Ⓧ╬ߩ໧㗴ࠍᛴ߃ߡ޿ࠆߩ߇⃻⁁ߢ޽ࠈ߁ޕᧄ ⎇ⓥߢឭ໒ߒߚᄙᲑဳ UASB ߦ߅޿ߡ߽ޔଐὼ SS ߩ⫾Ⓧߪߺࠄࠇࠆ߽ߩߩޔᓥ᧪ߩ UASB ᴺࠃࠅ߽࿕ᒻᚑಽߩ㒰෰࡮นṁൻଦㅴࠍታ⃻ߒߡ߅ࠅޔߘߩ໧㗴ὐࠍ⸃᳿ߔࠆߚ߼ߩᛛⴚะ ਄ߦᄢ߈ߊነਈߒߚߣ⠨߃ࠄࠇࠆޕᄙᲑဳ UASB ߦࠃࠆឃ᳓ಣℂᛛⴚߪޔ⇓↥ឃ᳓એᄖߦ߽ ᄙߊߩ㘩ຠ⵾ㅧ♽ߩឃ᳓ಣℂߦㆡ↪ߐࠇޔ⃻࿷ޔ㐿⊒ߩᦨ⚳Ბ㓏ߦ޽ࠆߣ޿߃ࠆޕ ߹ߚޔMAP ᨆ಴ᛛⴚߪޔMAP ઃ⌕ᶐẃ᧚ᢱࠍᛩ౉ߒߚ࡝ࠕࠢ࠲࡯ߥߤ߇㐿⊒ߐࠇޔ⎇ⓥ ↳⺧ᒰᤨࠃࠅ߽ޔ⇓↥ឃ᳓ߩಣℂ߅ࠃ߮᦭ଔ‛࿁෼ᛛⴚߣߒߡޔᐢߊ᥉෸ߒߟߟ޽ࠆࠃ߁ߢ ޽ࠆޕ 㧠㧚੹ᓟߩᣇะ࡮⺖㗴  ᚒޘߩ⎇ⓥࠣ࡞࡯ࡊߢߪޔᧄ੐ᬺ⚳ੌᓟ߽ޔᒁ߈⛯߈⇓↥ឃ᳓࡮ᑄ᫈‛㑐ଥߩࡔ࠲ࡦ⊒㉂ ಣℂߣ⊒㉂ᶧߩ⢈ᢱ೑↪╬ߩ⎇ⓥࠍㅴ߼ߡ޿ࠆޕ ⇓↥ᑄ᫈‛ߩࡔ࠲ࡦ⊒㉂ߩࡂ࡯࠼⊛⺖㗴ߢ޽ࠆ࿕ᒻಽߩนṁൻߩଦㅴߦߟ޿ߡߪޔᧄ੐ᬺ ߦ߅ߌࠆ⍮⷗ࠍ߽ߣߦޔࡄ࡯ࡓࠝࠗ࡞⵾ㅧᑄᶧߩࠃ߁ߥޔࠃࠅ㜞Ớᐲ࡮㜞 SS ฽᦭ឃ᳓ಣℂ ߳ߣߟߥ߇ࠅޔࡑ࡟࡯ࠪࠕߩ↢↥⃻႐ߢߩታ↪ൻ⹜㛎ߥߤࠍⴕߞߡ޿ࠆޕ߹ߚ࿕ᒻᑄ᫈‛ߩ Ḩᑼ߅ࠃ߮ੇᑼࡔ࠲ࡦ⊒㉂ಣℂᛛⴚߦߟ޿ߡ߽ᛛⴚᬌ⸽ࠍⴕߞߡ޿ࠆޕߎߩࠃ߁ߦޔࠃࠅ㜞 SS฽᦭ឃ᳓ߩಣℂ޽ࠆ޿ߪ࿕ᒻ‛ߩല₸⊛ᶖൻ࡮ࡔ࠲ࡦ⊒㉂ᛛⴚߩ⏕┙߇ޔ੹ᓟߩ⺖㗴ߣߒ ߡᒝߊᦸ߹ࠇߡ޿ࠆޕ ߐࠄߦޔ⇓↥♽ᑄ᫈‛ߩ⊒㉂ᶧ࡮ᱷᷲࠍ⢈ᢱߣߒߡ᦭ല೑↪ߔࠆ⠨߃ᣇߪޔ੹ᓟ߽㊀ⷐߥ⺖ 㗴ߢ޽ࠈ߁ޕᚒޘ߽ޔታⷙᮨߩ੃‐♽⇓↥ᑄ᫈‛ߩࡔ࠲ࡦ⊒㉂ࡊ࡜ࡦ࠻ߦߟ޿ߡޔ⊒㉂ᶧࠍ ⋥ធᣉ⢈ߔࠆߎߣࠍᔨ㗡ߦޔߘߩല₸⊛ᶖᲥᚻᴺߩ㐿⊒ߣ⢈ᢱߩⴡ↢ቇ⊛቟ోᕈ⹏ଔᚻᴺߩ ⏕┙╬ࠍ⋡ᜰߒߡ޿ࠆ(Uemura S., Harada H.ઁ:Production of biologically safe digested manure for land application by a full-scale biogas plant with heat-inactivation. Waste

Management and Research, 26, 247-255㗁, 2008.㧕ޕ

㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥ౏⴫ႎ๔╬

㧝㧕਄᧛❥᮸ޔේ↰⑲᮸ઁ㧦ᄙᲑဳ UASB ෻ᔕེߦࠃࠆ㜞Ớᐲ SS ฽᦭ឃ᳓ߩಣℂ․ᕈޔ ↪᳓ߣᑄ᳓ vol.43, No.11, 961-967, 2001.

㧞㧕Uemura S., Harada H.ઁ㧦Treatment of wastewater containing high amount of suspended solids by a novel multi-staged UASB reactor. Environmental Technology, vol.26, 1355-1362, 2005.

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࠙ࠪෆᲣ⚦⢩ߩ૕ᄖᚑᾫၭ㙃ᦼ㑆ߦ₪ᓧߔࠆ⢦⊒↢⢻ߦ෸߷ߔࡒ࠶

࠼ࠞࠗࡦߩലᨐߦ㑐ߔࠆ⎇ⓥߩㅴዷߣታ↪ൻ߳ߩዷᦸ

㧔੩ㇺᄢቇ ಎᢎ᝼ ጊ↰㓷଻㧕 ᐔᚑ 10㨪11 ᐕᐲᆔ⸤ޟູ੃േ‛ෆሶߩᚑᾫ߅ࠃ߮⢦⊒↢ߦኻߔࠆᯏ⢻⊛ၭ㙃ᴺߩ㐿⊒ޠ㧔੩ㇺᄢቇ ഥᢎ᝼㧕 ᐔᚑ 12㨪13 ᐕᐲᆔ⸤ޟ࠙ࠪෆሶ⚦⢩⾰ᚑᾫߩଦㅴߦ㑐ࠊࠆಽሶၮ⋚ޠ㧔੩ㇺᄢቇ ഥᢎ᝼㧕 㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ  ࠙ࠪߩല₸⊛Ⴧ↥ߦ߅޿ߡޔዼ૕޽ࠆ޿ߪ↢૕ౝߩෆᎽෆ⢩߆ࠄណขߒߚᧂᚑᾫෆᲣ⚦⢩ ࠍ૕ᄖᚑᾫၭ㙃ߦࠃߞߡฃ♖น⢻ߥ⁁ᘒ߹ߢᚑᾫߐߖߚᓟߦޔ૕ᄖฃ♖߅ࠃ߮૕ᄖၭ㙃ߦࠃ ߞߡ⒖ᬀน⢻ߥ⢦⋚⢩ࠍ↢↥ߔࠆ৻ㅪߩၭ㙃ᛛⴚ߇㐿⊒ߐࠇޔ᦭ല೑↪ߐࠇߡ޿ࠆޕߒ߆ߒޔ ᓥ᧪ߩࠃ߁ߥ૕ᄖၭ㙃ᛛⴚߦࠃߞߡෆᲣ⚦⢩߆ࠄ૞಴ߐࠇࠆ⢦⋚⢩ߩഀวߪޔ↢૕ౝ↱᧪⢦ ߦᲧߴߡ᣿ࠄ߆ߦૐ޿ߎߣ߇໧㗴ߣߥߞߡ޿ࠆޕෆᲣ⚦⢩ߩ૕ᄖၭ㙃ߦࠃࠆ⢦↢↥ߩߐࠄߥ ࠆല₸ൻࠍ࿑ࠆߚ߼ߦߪޔ⢦⋚⢩ᦼ߳⊒↢ߔࠆ⢦ߩ⢻ജ߇ฃ♖೨ߩෆᲣ⚦⢩ߩ⚦⢩⾰ᚑᾫ(ෆ ⚦⢩⾰ᚑᾫ)ߦࠃߞߡਥߦ₪ᓧߐࠇࠆߎߣ߆ࠄޔ࠙ࠪෆᲣ⚦⢩ߩ૕ᄖᚑᾫၭ㙃ᛛⴚߩᡷༀ߇ᭂ ߼ߡ㊀ⷐߣߥࠆޕ  ෆᲣ⚦⢩ߩᚑᾫߪޔᕈ⣼ೝỗࡎ࡞ࡕࡦߛߌߢߥߊޔ㗰☸ጀ⚦⢩ޔෆ⢩ᶧ߅ࠃ߮ෆਐ⚦⢩ෆ Უ⚦⢩ⶄว૕(COC)߆ࠄ᭴ᚑߐࠇࠆෆ⢩ౝߩዪᚲᕈ࿃ሶߦࠃߞߡ⺞▵ߐࠇߡ޿ࠆޕᚒޘߪޔ ෆ⢩ᶧਛߦሽ࿷ߔࠆᣂⷙࡋࡄ࡝ࡦ⚿วᕈᚑ㐳࿃ሶߢ޽ࠆࡒ࠶࠼ࠞࠗࡦ(MK)߇࠙ࠪෆ⚦⢩⾰ ᚑᾫଦㅴ࿃ሶߢ޽ࠆߎߣޔ߅ࠃ߮ߘߩ૞↪ᯏᐨࠍએਅߩ⎇ⓥ߆ࠄ᣿ࠄ߆ߦߒߚޕ ߹ߕޔ࠙ࠪ MK(bMK)cDNA ߩࠢࡠ࡯࠾ࡦࠣߣ࡝ࠦࡦࡆ࠽ࡦ࠻ bMK ߩ૞⵾ࠍ⹜ߺߚޕߘ ߩ⚿ᨐޔbMK cDNA ߆ࠄ੍ᗐߐࠇࠆ bMK(ಽሶ㊂⚂ 16kDa)ߩࠕࡒࡁ㉄㈩೉ߪޔࡑ࠙ࠬ߅ࠃ ߮ࡅ࠻ MK ߣ㜞޿⋧หᕈࠍ␜ߒޔ10 ୘ߩࠪࠬ࠹ࠗࡦᱷၮ߇࠙ࠪޔࡑ࠙ࠬޔࡅ࠻ߩ㧟⒳㑆ߢޔ 30 ୘ߩႮၮᕈࠕࡒࡁ㉄ᱷၮ߇ࡅ࠻ߣ࠙ࠪߩ㑆ߢ଻ሽߐࠇߡ޿ࠆ(࿑㧝)ߎߣޔߐࠄߦޔ᣸⯻⚦ ⢩⊒⃻♽ߦࠃߞߡ࡝ࠦࡦࡆ࠽ࡦ࠻ bMK ࠍ㜞޿෼₸ߢ૞⵾ߢ߈ࠆߎߣ߇ࠊ߆ߞߚޕ ᰴߦޔ૞⵾ߒߚ࡝ࠦࡦࡆ࠽ࡦ࠻ bMK ߩ࠙ࠪෆ⚦⢩⾰ᚑᾫߦ෸߷ߔലᨐࠍޔ૕ᄖฃ♖ᓟߩ ෆഀ߅ࠃ߮⢦⋚⢩߳ߩ⊒↢₸ߦࠃࠅᬌ⸛ߒߚޕbMK(200ng/ml)ࠍᷝടߒߚᚑᾫၭ࿾ߢ࠙ࠪ COCࠍၭ㙃ߔࠆߣޔᩭߩᚑᾫߣ૕ᄖฃ♖ᓟߩෆഀߪᓇ㗀ࠍฃߌߥ޿߇ޔ⢦⋚⢩߳ߩ⊒↢₸߇ ήಣℂኻᾖ඙ߦᲧߴߡ᦭ᗧߦ㜞ߊߥߞߚ(ಣℂ඙:50%ޔήಣℂ 21%ޔP<0.05㧕ޕ߹ߚޔCOC ߆ࠄෆਐ⚦⢩ࠍ㒰෰ߒߚ⵻ൻෆᲣ⚦⢩(DO)ࠍ bMK ᷝടᚑᾫၭ࿾ߢၭ㙃ߒߡ߽ޔ૕ᄖฃ♖ᓟޔ ⢦⋚⢩߳ߪోߊ⊒↢ߒߥ߆ߞߚ߇ޔෆਐ⚦⢩ߣߩ౒ၭ㙃ߢ૕ᄖᚑᾫࠍⴕߞߚ႐วޔbMK ߦࠃ ࠅ DO ߩ⢦⋚⢩߳ߩ⊒↢߇ଦㅴߐࠇߚޕߐࠄߦޔbMK ᷝട޽ࠆ޿ߪήᷝടၭ࿾ߢၭ㙃ߒߚෆ ਐ⚦⢩ߩ㚔ൻၭ࿾(CMMK+ޔCMMK-)ࠍ⺞ᢛߒޔDO ߩ⚦⢩⾰ᚑᾫߦ෸߷ߔߘࠇߙࠇߩ㚔ൻ ၭ࿾ߩലᨐࠍᬌ⸛ߒߚ⚿ᨐޔDO ߩ⚦⢩⾰ᚑᾫଦㅴᵴᕈߪ CMMK+ߦߩߺሽ࿷ߒޔߘߩᵴᕈ ߪᾲ(100͠)߅ࠃ߮࠻࡝ࡊࠪࡦಣℂߦࠃߞߡᄬᵴߒߚޕ એ਄ߩ⚿ᨐ߆ࠄޔbMK ߩෆ⚦⢩⾰ᚑᾫଦㅴലᨐߪޔෆਐ⚦⢩߆ࠄಽᴲߐࠇࠆࡐ࡝ࡍࡊ࠴࠼ ᕈߩᶧᕈ࿃ሶࠍ੺ߒߡ⊒ើߐࠇࠆߎߣ߇␜ໂߐࠇߚޕ߹ߚޔCOC ߩ૕ᄖᚑᾫၭ㙃ᦼ㑆ߦޔෆ ਐ⚦⢩ߩࠕࡐ࠻࡯ࠪࠬᄌᕈ߇⿠ߎࠆߎߣ߇⍮ࠄࠇߡ޿ࠆ߇ޔbMK ಣℂߦࠃࠅࠕࡐ࠻࡯ࠪࠬᄌ ᕈߪᛥ೙ߐࠇࠆߎߣ߇ޔTunnel ᨴ⦡߅ࠃ߮ LM-PCR ᴺߦࠃߞߡ᣿ࠄ߆ߣߥߞߚ2)ޕᓥߞߡޔ bMKߪෆਐ⚦⢩ߩ↢ሽᕈߣᯏ⢻ޔߟ߹ࠅෆ⚦⢩⾰ᚑᾫଦㅴ࿃ሶߩಽᴲᯏ⢻ࠍ㜞߼ࠆߎߣߦࠃ ߞߡޔ࠙ࠪෆᲣ⚦⢩ߩ⚦⢩⾰ᚑᾫߦኻߔࠆଦㅴലᨐࠍ⊒ើߒߡ޿ࠆߣ⠨߃ࠄࠇߚޕ

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㧞㧚⎇ⓥ೔㆐ߩ⃻⁁  ෆᲣ⚦⢩ߩ⚦⢩⾰ᚑᾫଦㅴߦ߅޿ߡޔෆਐ⚦⢩ߣߩ⋧੕૞↪߇㊀ⷐߥᓎഀࠍᨐߚߒߡ޿ࠆ ߇ޔߘߩ⋧੕૞↪ߦ㑐ߔࠆ⎇ⓥߪޔ࿖ౝߢߪ✜ߦዞ޿ߚ߫߆ࠅߢ޽ࠆޕᚒޘߩ⎇ⓥߢߪޔ࡝ ࠦࡦࡆ࠽ࡦ࠻ bMK ࠍ᣸⯻⚦⢩⊒⃻♽ߢᄢ㊂ߦ૞⵾ߔࠆߎߣ߇น⢻ߣߥߞߚߎߣ߆ࠄޔbMK ߩ࠙ࠪෆ⚦⢩⾰ᚑᾫଦㅴᯏ᭴ߩ⸃᣿ߦ㑐ߔࠆ⎇ⓥ߇ㅴዷߒޔbMK ߩ૞↪ࠍฃߌߚෆਐ⚦⢩߆ ࠄಽᴲߐࠇࠆෆ⚦⢩⾰ᚑᾫଦㅴ࿃ሶߩត⚝ࠍ㐿ᆎߒߡ޿ࠆޕbMK ಣℂߦࠃߞߡෆਐ⚦⢩ߢࠃ ࠅᒝߊ⊒⃻ߔࠆㆮવሶࠍࠨࡉ࠻࡜࡚ࠢࠪࡦᴺߢษࠅ਄ߍࠆߎߣࠍ⹜ߺޔ5 ߟߩㆮવሶࠍหቯ ߒߚޕ੹ᓟޔߎࠇࠄߩㆮવሶ↥‛ߣෆ⚦⢩⾰ᚑᾫଦㅴᵴᕈߣߩ㑐ଥࠍᬌ⸛ߔࠆ੍ቯߢ޽ࠆޕ 㧟㧚ᛛⴚታ↪ൻߩ⃻⁁  bMK ᷝടၭ࿾ࠍ↪޿ߚ࠙ࠪ COC ߩ૕ᄖᚑᾫၭ㙃♽߇㐿⊒ߐࠇߚߎߣߦࠃߞߡޔ࠙ࠪ૕ᄖ ฃ♖⢦↢↥ᛛⴚߪᡷༀߐࠇߚޕߒ߆ߒޔDO ߆ࠄߩ⢦↢↥ߦ߅޿ߡߪޔbMK ಣℂၭ㙃ෆਐ⚦ ⢩ߩ㚔ൻၭ࿾ਛߦ DO ߩෆ⚦⢩⾰ᚑᾫࠍଦㅴߔࠆᵴᕈ࿃ሶ߇ሽ࿷ߔࠆߎߣ߇⏕⹺ߐࠇߡ޿ࠆ ߩߺߢޔߘߩᵴᕈ࿃ሶߪหቯߐࠇߡ޿ߥ޿ޕߎߩᵴᕈ࿃ሶ߇หቯߐࠇޔᣂⷙߩ૕ᄖᚑᾫၭ㙃 ᛛⴚ߇㐿⊒ߐࠇࠇ߫ޔෆᲣ⚦⢩๟࿐ߩෆਐ⚦⢩ߩઃ⌕⁁ᘒߦ߆߆ࠊࠄߕޔ੹߹ߢએ਄ߦല₸ ⊛ߥෆᲣ⚦⢩߆ࠄߩ⢦↢↥߇น⢻ߣߥࠅޔ⇓↥ᬺ߫߆ࠅߢߥߊޔ૕ᄖᚑᾫෆᲣ⚦⢩ߩ૕ᄖฃ ♖߿㗼ᓸ᝼♖ߦࠃࠆਇᅧᴦ≮ࠍⴕ߁↢ᱺක≮ಽ㊁ߦ߽⽸₂ߔࠆߎߣ߇ᦼᓙߐࠇࠆޕ 㧠㧚੹ᓟߩᣇะޔ⺖㗴  ෆᲣ⚦⢩ߩ⚦⢩⾰ᚑᾫߩ⺞▵ߦ߅޿ߡޔෆਐ⚦⢩ߣෆᲣ⚦⢩㑆ߩ⋧੕૞↪߇ᭂ߼ߡ㊀ⷐߥ ᓎഀࠍᨐߚߒߡ޿ࠆޕ․ߦޔෆਐ⚦⢩߆ࠄෆᲣ⚦⢩ߦ⋥ធ૞↪ߒߡ⚦⢩⾰ᚑᾫࠍଦㅴߔࠆᶧ ᕈ࿃ሶࠍන㔌࡮หቯߔࠆߎߣ߇ޔCOC ߩߺߥࠄߕ⵻ൻෆᲣ⚦⢩߆ࠄ࠙ࠪ⢦ࠍ㜞ല₸⊛ߦ↢↥ ߔࠆ૕ᄖၭ㙃ᛛⴚߩ㐿⊒ߦ߅޿ߡޔᦨ߽㊀ⷐߥ⎇ⓥ⺖㗴ߣ⠨߃ࠄࠇࠆޕߎߩ⺖㗴ࠍ⸃᳿ߔࠆ ౕ૕╷ߣߒߡޔbMK ಣℂၭ㙃ෆਐ⚦⢩ߩ㚔ൻၭ࿾ߦሽ࿷ߔࠆ࠙ࠪ⵻ൻෆᲣ⚦⢩ߩ⚦⢩⾰ᚑᾫ ଦㅴ࿃ሶࠍන㔌࡮หቯߔࠆ⎇ⓥ߇᜼ߍࠄࠇࠆޕ 㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥ౏⴫ႎ๔╬

㧝㧕Ikeda S., et al.㧦Mol. Reprod. Dev., 57: 99-107, 2000. 㧞㧕Ikeda S., et al.㧦Reproduction, 132: 549-557, 2006. 㪈 㪈㪌 㪊㪐 㪉㪊 㪊㪇 㪢 㪢 㪢 㪢 㪢 㪢 㪚 㪚 㪚 㪩 㪚 㪚 㪋㪏 㪌㪉 㪚 㪢 㪢 㪚 㪍㪉 㪢 㪢 㪚 㪢 㪩 㪎㪉 㪢 㪢 㪚 㪐㪋 㪚 㪢 㪈㪇㪋 㪢 㪢 㪢 㪢 㪢 㪢 㪈㪉㪈 㪤 㪫 㪢 㪩 㪘 㪩 㪩 㪢 㪪 㪜 䉡䉲䊚䉾䊄䉦䉟䊮䈱৻ᰴ᭴ㅧ 㪩 㪛 㪞 㪧 㪞 㪘 㪭 㪠 㪫 㪞 㪧 㪘 㪘 䊶㪊㪇୘䈱Ⴎၮᕈ䉝䊚䊉㉄䈲㪿㪤㪢䈫ห৻㩷㩿㩷㩷㩷㩷㩷㪀 䊶㪈㪇୘䈱䉲䉴䊁䉟䊮㩷㩿㩷㩷㩷㩷㩷㪀㩷 㪞 㪪 㪜 㪜 㪮 㪮 㪞 㪫 㪧 㪪 㪪 㪞 㪞 㪝 㪜 㪞 㪫 㪞 㪘 㪨 㪫 㪨 㪭 㪧 㪥㪮 㪜 㪝 㪞 㪘㪛 㪰 㪝 㪜 㪮 㪞 㪘 㪛 㪞 㪫 㪞 㪫 㪨 㪞 㪫 㪣 㪘 㪰 㪥 㪘 㪨 㪨 㪜 㪫 㪠 㪭 㪫 㪧 㪫 㪘 㪞 㪛 ࿑㧝 ࠙ࠪࡒ࠶࠼ࠞࠗࡦߩ৻ᰴ᭴ㅧ ࡮ 30 ୘ߩႮၮᕈࠕࡒࡁ㉄ߪࡅ࠻ࡒ࠶࠼ࠞࠗࡦߣห৻㧔 㧕 ࡮ 10 ୘ߩࠪࠬ࠹ࠗࡦ㧔 㧕

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㘩ᢱ⥄⛎₸ะ਄ߩߚ߼ߩᨋ㑆᡼’ࠪࠬ࠹ࡓߩౣ᭴▽

㧔᧲ർᄢቇᄢቇ㒮 ᢎ᝼ ૒⮮ⴐ੺㧕 ᐔᚑ 12㨪13 ᐕᐲᆔ⸤ޟૐࠦࠬ࠻ᄢⷙᮨᨋ㑆᡼’ࠪࠬ࠹ࡓߩ⃻ઍ⊛ౣ᭴▽ޠ㧔᧲ർᄢቇ ഥᢎ᝼㧕 㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ  㘩ᢱߩ⥄⛎₸ะ਄ߪޔㄘ᡽ߩᦨᄢߩ⺖㗴ߢ޽ࠆޕ⇓↥ߦ߅޿ߡߪޔ㘺ᢱ⥄⛎₸ߩะ਄߇ߘ ߩ೨ឭߣߥࠆ߇ޔᧂߛߦ 24㨪25%ߢផ⒖ߒߡ߅ࠅޔ޽ࠄࠁࠆ㘺ᢱ⥄⛎ࠪࠬ࠹ࡓߩឭ᩺߇ᦼᓙ ߐࠇߡ޿ࠆޕㄭᐕߩⓃ‛ଔᩰ㜞㛛ߪ㔛⛎㑐ଥߩ᭴ㅧ⊛໧㗴߆ࠄ⊒ߒߡ߅ࠅޔߎߩⷐ⺧ߪᧂߛ ߦ੹ᣣ⊛⺖㗴ߢ޽ࠆޕᧄ⎇ⓥߢߪޔ᫪ᨋߩਅ⨲ߣߒߡ⼾ንߦሽ࿷ߔࠆᬀ‛ࠍޔ‐ߩ㘺ᢱ⾗Ḯ ߣߒߡ೑↪ߔࠆࠪࠬ࠹ࡓߩ㐿⊒ࠍ⋡⊛ߣߒߚޕ ᨋౝ᡼’ߪᭂ߼ߡฎ޿ᱧผࠍᜬߟ߇ޔ᣿ᴦ 30 ᐕઍߦㅧᨋ੐ᬺ߇㐿ᆎߐࠇࠆߎߣߦࠃࠅޔ᫪ ᨋ߆ࠄ‐㚍ߪㅊ޿಴ߐࠇޔᄢᱜ 5 ᐕ(1916 ᐕ)ߩ㚍↥ଏ↪㒢ቯ࿾೙ᐲߩਛߢޔᨋౝ᡼’ߪߐࠄ ߦ㒢ቯߐࠇࠆߎߣߣߥߞߚޕㄭᐕ(1998 ᐕ)ߩ⺞ᩏߢߪޔ᡼’౒↪ᨋ㊁(౮⌀㧝)ߪ 1.6 ਁ ha ߦ ߹ߢ⪭ߜㄟߺޔߐࠄߦᷫዋߩ৻ㅜࠍㄡߞߡ޿ࠆޕߒ߆ߒޔ⡺↪❥ᱺ‐ߩᩕ㙃ⷐ᳞㊂ߪૐ޿ߚ ߼ޔߘߩ㘺㙃ߦߣߞߡᨋ࿾ߩਅ⨲ߪᧂߛߦ㝯ജ⊛ߢ޽ࠆޕ㉿ጊߢ޽ࠆᣥ⮌὇ᨋ߇ 618 ਁ haޔ ᅏጊߢ޽ࠆ᡼’౒↪ᨋ㊁߇ 1.6 ਁ ha ߣߔࠆߣޔ⡺↪⒳❥ᱺ‐ 63.6 ਁ㗡ߪో㗡ߎߎߢ㘺⢒ߢ ߈ࠆޕ‐⟲ߩ⋙ⷞࠍޔ⋙ⷞੱߦࠃࠆᾫ✵ߒߚᛛ⢻߆ࠄ⑼ቇ⊛࠺࡯࠲ߦၮߠߊᛛⴚߦ᥉ㆉൻߒޔ ߆ߟઁߩ↢ᘒ♽ࠨ࡯ࡆࠬ(↢‛ᄙ᭽ᕈߥߤߩᄙ㕙⊛ᯏ⢻)߽ᗧ⼂ߒߚᨋ㑆᡼’ࠪࠬ࠹ࡓ㐿⊒߇ ᧄ⎇ⓥߩౝኈߢޔᬀᨋᐕ߇⇣ߥࠆ㊎⪲᮸ੱᎿᨋ⃰(ࠬࠡޔࠞ࡜ࡑ࠷)ߣੱᎿᨋߩ㒐㘑↪ߣߒߡߘ ࠇࠄࠍ࿐߁ᐢ⪲᮸ᨋ⃰(ࡉ࠽ޔࡒ࠭࠽࡜╬)߇ࡕࠩࠗࠢ⁁ߦሽ࿷ߔࠆౖဳ⊛ߥㄭઍ⊛ᣉⴕᴺߩ᫪ ᨋߢߩᚑᨐߢ޽ࠆޕ 㪇 㪈 㪇 㪇 㪉 㪇 㪇 㪊 㪇 㪇 㪋 㪇 㪇 㪇 㪌 㪈 㪇 㪈 㪌 㪉 㪇 ᬀᨋᓟ䈱⚻ㆊᐕᢙ 㪾㪛 㪤 㪆㫄 㪉 ࿑㧝 ᡼’౒↪ᨋ㊁         ࿑㧞 ᬀᨋᓟߩ⚻ㆊᐕᢙߣน㘩ㇱ⃻ሽ㊂ߣߩ㑐ଥ                    Y = 32.6 + 14.9X (Y=gDM/m2; X=⚻ㆊᐕᢙ; r=0.9, p<0.001) น㘩ᬀ‛⃻ሽ㊂ߩട㊀ᐔဋ୯:133gDM/m2 㧞㧚⎇ⓥ೔㆐ߩ⃻⁁ 㧝㧕ᨋ࿾ߩਅጀߦߪᄙ㊂ߩน㘩ᬀ‛߇ 5 ᦬߆ࠄ 10 ᦬ߩ᡼’ᦼ㑆ਛ⼾ንߦሽ࿷ߔࠆߎߣޔ߹ ߚޔᐕ㑆ࠍㅢߓߚ᡼’࿾ో૕ߩ CP ฽㊂ߪ 11.9%ߢ޽ࠆߎߣ߆ࠄޔᨋ࿾ߪ‐ߩ㙄႐ߣߒߡߩ ㊂⊛࡮⾰⊛ᔅⷐ᧦ઙࠍ߶߷ḩߚߔߣ⠨߃ࠄࠇࠆ(᧖ጊࠄ(2004))ޕ 㧞㧕‐ฦ୘૕ߪޔᐔဋ 13㨪14ha/ᣣࠍ೑↪ߔࠆ(࿑㧟)ޕߘߩ㕙Ⓧߪޔਅጀᬀ‛ߩ CP ฽㊂߇ ᦨ߽㜞޿ᤨᦼߢ޽ࠆ 5࡮6 ᦬ߦᐢߊޔCP ฽㊂߅ࠃ߮☻❫⛽(CF)฽㊂߇ᦨ߽ૐ޿ᤨᦼߢ޽ࠆ 10 ᦬ߦ⁜ߊߥࠆޕᦨ߽೑↪㕙Ⓧ߇⁜ߊߥࠆ 10 ᦬ߦߪޔ‐ߪዊ⟲ߦಽ߆ࠇޔᢔ㐿ߔࠆߎߣ(࿑㧠) ߆ࠄ⋙ⷞߪ࿎㔍ߦߥࠅ(૒⮮(2005)ޔ቟ᳯ࡮૒⮮(2005))ޔߘߩቄ▵એ೨ߢߩ⚳’߇ᔅⷐߢ޽ࠆޕ ੱᎿᨋ ᐢ⪲᮸ᨋ ⶄጀᨋ

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㧟㧕ߎߩࠃ߁ߥᨋ㑆᡼’ߪޔ࿯ფേ‛⋧߿㊁↢േ‛⋧ߩᄙ᭽ᕈߦᓇ㗀ߪߥߊޔࡃ࡜ࡦࠬߩ ขࠇߚᜬ⛯⊛↢ᘒ♽ࠨ࡯ࡆࠬ߇ឭଏߢ߈ࠆࠪࠬ࠹ࡓߢ޽ࠆߎߣ߇⏕⹺ߐࠇߚޕ 㪇 㪌 㪈 㪇 㪈 㪌 㪉 㪇 㪉 㪌 㪌 ᦬ 㪍 ᦬ 㪎 ᦬ 㪏 ᦬ 㪐 ᦬ 㪈 㪇 ᦬ 㪿 㪸 㪉 㪇 㪇 㪇 㩿㪉 㪀 㪉 㪇 㪇 㪈 㩿㪌 㪀   㪇 㪉 㪇 㪇 㪋 㪇 㪇 㪍 㪇 㪇 㪏 㪇 㪇 㪌 ᦬ 㪏 ᦬ 㪈 㪇 ᦬ 㪿㪸 㪈㪐㪐㪎 㪈㪐㪐㪏 ࿑㧟 㧝ᣣⴕേ࿤㕙Ⓧߩ᦬㑆ᄌേ      ࿑㧠 ో㗡ⴕേ࿤㕙Ⓧߩቄ▵㑆Ꮕ 㧟㧚ᛛⴚታ↪ൻߩ⃻⁁  ࠬࠡੱᎿᨋ 50 ᐕબᦼᣉⴕߦࠃࠆᜬ⛯⊛ᨋ㑆᡼’ࠪࠬ࠹ࡓࠍឭ᩺ߒߚޕࡉ࠽࡮ࡒ࠭࠽࡜ࠍਛ ᔃߣߒߚᐢ⪲᮸ᨋߦ࿐߹ࠇߚ 1 ᨋ⃰ 10ha ࠍන૏ߣߒߡᲤᐕᬀᨋࠍታᣉߒޔ50 ᐕᓟߦ෼ⓠߔ ࠆࠪࠬ࠹ࡓߢ޽ࠆޕ500ha ߩੱᎿᨋߦ 208DM ࠻ࡦޔߘࠇࠍขࠅᏎߊ 300ha ߩᐢ⪲᮸ᨋߦ 945DM࠻ࡦߩน㘩ᬀ‛⃻ሽ㊂߇ሽ࿷ߒޔߘߎߢߪ 30 㗡ߩ❥ᱺ‐⟲ࠍᜬ⛯⊛ߦ 170 ᣣ㑆᡼’ ߢ߈ࠆޕᔅⷐੇ‛ⷐ᳞㊂ߪ 45DM ࠻ࡦߢ޽ࠆ߇ޔᜬ⛯⊛೑↪ࠍ⠨߃ߚ႐วߪޔ೑↪₸ࠍ 4% ⒟ᐲߣߔࠆޕ 㧠㧚੹ᓟߩᣇะ࡮⺖㗴  ᧄᛛⴚߪޔ߈ࠊ߼ߡ዁᧪ᕈߩ㜞޿ᛛⴚߢ޽ࠆޕᓥ᧪ߩ㘩ᢱଏ⛎ࠨ࡯ࡆࠬߩߺߦ․ൻߒߚ↢ ᘒ♽ߩ೑↪ߢߪޔ੹ࡒ࡟࠾ࠕࡓࠍਸ਼ࠅಾࠇߥ޿ߎߣ߇ޔ࿖ㅪⅣႺ⸘↹ߩ੐ോዪ߆ࠄ 2005 ᐕߦ ಴ ߐࠇߚޟ↢ᘒ♽ࠨ࡯ࡆࠬߣੱ㘃ߩ዁᧪ޠߢ᣿ࠄ߆ߣߥߞߚޕଏ⛎ࠨ࡯ࡆࠬߦട߃ޔၮ⋚(࿯ ფ╬)ޔ⺞ᢛ(᳇୥࡮ᵩ᳓╬)ޔᢥൻߩฦࠨ࡯ࡆࠬࠍࡃ࡜ࡦࠬࠃߊឭଏߢ߈ࠆ᡼’ࠪࠬ࠹ࡓ᭴▽ ߩ╙㧝ᱠߣߒߡޔᧄᛛⴚࠍ⹏ଔߔࠆᔅⷐ߇޽ࠆޕ 㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥ౏⴫ႎ๔╬ 㧝㧕૒⮮ⴐ੺㧦ᅏጊ࿖᦭ᨋߩή’ᩋ⡺↪‐᡼’೑↪ࠪࠬ࠹ࡓߩឭ᩺ޔ㑐᧲⇓↥ቇળႎ 55(2):115-119. 2005. 㧞㧕᧖ጊߺߤࠅ࡮૒⮮ⴐ੺࡮቟ᳯஜ࡮಴ญༀ㓉࡮⩲ේ๺ᄦ㧦ᅏ⠀ጊ♽᡼’౒↪ᨋ㊁ߦ߅ߌ ࠆน㘩ㇱ⃻ሽ㊂߅ࠃ߮ᩕ㙃ଔߩಽᏓޔᣣᧄ⨲࿾ቇળ⹹ 50(3):294-299. 2004. 㧟㧕቟ᳯஜ࡮૒⮮ⴐ੺㧦ή’ᩋᨋ㑆᡼’ਅߢߩኅ⇓࠙ࠪߩណ㙄ⴕേߣ▤ℂޔູ੃㘃⑼ቇ 45(1):105-110. 2005. a b b ab b b

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ᚑ 䇭‐ 䋱䋳ㅳ㦂 䋳ㅳ㦂 ╙৻⢗ ╙྾⢗ චੑᜰ⣺ ╙৻⢗ 㔌੃ 䊚䊦䉪 䊚䊦䉪䋫 㪭㪝㪘 䇭䇭䇭䇭䇭࿑䋱䇭ᶖൻ▤䈱䊧䊒䉼䊮⊒⃻䈮䈍䈔䉎䌖䌆䌁䈱૞↪ ᚑ 䇭‐ 䋱䋳ㅳ㦂 䋳ㅳ㦂 ╙৻⢗ ╙྾⢗ චੑᜰ⣺ ╙৻⢗ 㔌੃ 䊚䊦䉪 䊚䊦䉪䋫 㪭㪝㪘 ᚑ 䇭‐ 䋱䋳ㅳ㦂 䋳ㅳ㦂 ╙৻⢗ ╙྾⢗ චੑᜰ⣺ ╙৻⢗ 㔌੃ 䊚䊦䉪 䊚䊦䉪䋫 㪭㪝㪘 䇭䇭䇭䇭䇭࿑䋱䇭ᶖൻ▤䈱䊧䊒䉼䊮⊒⃻䈮䈍䈔䉎䌖䌆䌁䈱૞↪

ሶ࠙ࠪߩઍ⻢೙ᓮᛛⴚߩㅴዷߣ໡ຠ㐿⊒

㧔᣿ᴦ㘺♳ᩣᑼળ␠ ᳓ᚭ⎇ⓥ’႐ ⎇ⓥ㘈໧ ዊේཅᤘ㧕 ᐔᚑ 12㨪13 ᐕᐲᆔ⸤ޟሶ࠙ࠪߩ៊⠻㒐ᱛߣ⊒⢒ଦㅴߦኻߔࠆᩕ㙃↢ℂቇ⊛ੱὑ೙ᓮޠ㧔᧲ർᄢቇ ᢎ᝼㧕 㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ  ູ੃ሶ࠙ࠪߪޔ↢ᓟ 4㨪6 ㅳ㑆ߢ╙৻⢗߇㛳⇣⊛ߦ⊒㆐ߒޔૐ⚖⢽⢌㉄(VFA)ߩ⊒㉂߇ᆎ߹ ࠆߥߤᩕ㙃↢ℂቇ⊛ߦᄢ߈ߥᄌൻࠍฃߌࠆޕߎߩᤨᦼߩㆡಾߥ㘺㙃▤ℂߪޔሶ࠙ࠪߩஜోߥ ⊒⢒ࠍଦㅴߒޔ੃࡮⡺ߩ↢↥ᕈࠍะ਄ߐߖࠆߎߣ߇ᦼᓙߢ߈ࠆޕᚒޘߪޔ╙৻⢗ౝߢ↥↢ߐ ࠇࠆ෻⧖ኅ⇓ߩਥⷐߥࠛࡀ࡞ࠡ࡯Ḯߢ޽ࠆ VFA ߦᵈ⋡ߒߡޔੱὑ⊛ߦ VFA ࠍ೙ᓮߔࠆߎߣ ߦࠃࠅሶ࠙ࠪߩᶖൻޔౝಽᴲޔઍ⻢ᯏ⢻ࠍ㜞߼ߡޔ៊⠻₸ࠍૐਅߐߖࠆߣหᤨߦޔೋᦼ૕㊀ ߩિ߮ࠍଦㅴߐߖޔ߭޿ߡߪ↢↥ᕈࠍჇᄢߐߖࠆߎߣࠍ⋡⊛ߣߒߡᧄ⎇ⓥࠍ㐿ᆎߒߚޕߘߩ ⚿ᨐޔሶ࠙ࠪߦ߅޿ߡߪޔVFA ߇╙৻⢗☼⤑ߩ ὇㉄⣕᳓㉂⚛(VFA ߩๆ෼ߦ㊀ⷐߥᓎഀࠍᨐߚ ߔ)ᵴᕈࠍ᦭ᗧߦჇടߐߖޔ╙৻⢗߆ࠄߩ VFA ߩๆ෼⢻ࠍჇടߐߖߡ޿ࠆߎߣޔណ㘩೙ᓮߦ㊀ ⷐߥᓎഀࠍᨐߚߔ࡟ࡊ࠴ࡦ߿ࠦ࡟ࠪࠬ࠻ࠠ࠾ ࡦߩ╙৻⢗ߥߤߩᶖൻ▤ߢߩㆮવሶ⊒⃻߇ VFA ߦࠃࠅ೙ᓮߐࠇߡ޿ࠆߎߣ(࿑ 1)ߥߤޔ VFA߇ሶ࠙ࠪߩណ㘩⺞▵ޔᶖൻޔઍ⻢ޔౝಽᴲ ᯏ⢻ߦ㊀ⷐߥᓎഀࠍᨐߚߒߡ޿ࠆߎߣࠍ᣿ࠄ ߆ߦߒߚޕ߹ߚޔሶ࠙ࠪ߳ߩࡊࡠࡇࠝࡦ㉄Ⴎߩ ⛎ਈߪޔ♧ઍ⻢߿⤟ౝಽᴲ♽ߦᓇ㗀ߒޔ༵ᅢᕈ࡮ஜᐽ⁁ᘒ߽⦟ᅢߦ଻ߟߎߣࠍ⏕⹺ߒߚޕ 㧞㧚⎇ⓥ೔㆐ߩ⃻⁁  వߦޔᚒޘ(1991)ߪޔ෻⧖ኅ⇓߳ߩᤃ⊒㉂ᕈߩ ὇᳓ൻ‛ߩᷝടߪޔ╙৻⢗ౝߩࡊࡠࡇࠝࡦ㉄Ớᐲ ࠍჇടߐߖޔ⢄ߦ߅ߌࠆ♧ᣂ↢ࠍ੺ߒߡ૕ౝߩࠣ ࡞ࠦ࡯ࠬߩ೑↪ᕈࠍჇടߐߖࠆߎߣޔ♧Ḯᕈࠕࡒ ࡁ㉄ࠍⰮ⊕⾰วᚑߦ೑↪ߒߡ޿ࠆߎߣޔዩ⚛ౣᓴ Ⅳᯏ⢻ࠍᵴᕈൻߒߡ⓸⚛ࠍ▵⚂ߒߡ޿ࠆߎߣࠍ ႎ๔ߒߚ(࿑㧞)ޕ߹ߚޔ㗇⮮ࠄ(1994)ߪޔ෻⧖ኅ ⇓߳ߩࡊࡠࡇࠝࡦ㉄Ⴎߩ⛎ਈߦ߅޿ߡ߽ޔ♧ᣂ↢ ߦࠃࠅ♧ઍ⻢ࠍᵴᕈൻߔࠆߣߣ߽ߦޔ⓸⚛ߩ೑↪ ᕈࠍ㜞߼ࠆߎߣࠍ᣿ࠄ߆ߦߒߚޕߎߩࠃ߁ߦޔ╙ ৻⢗ౝߢߩࡊࡠࡇࠝࡦ㉄⊒㉂߇෻⧖ኅ⇓ߩᩕ㙃 ↢ℂߦ߅޿ߡ㊀ⷐߥᓎഀࠍᨐߚߒߡ޿ࠆߎߣ߇ ᜰ៰ߐࠇߡ޿ࠆޕᧄ⎇ⓥߢᓧࠄࠇߚሶ࠙ࠪߦ߅ߌࠆ VFA ߩᩕ㙃↢ℂቇ⊛․ᕈߦ㑐ߔࠆᚑᨐࠍ ↢߆ߒޔታ↪ൻߦะߌߡ⎇ⓥࠍⴕ߁႐วޔᶖൻ▤ߦᏱ࿷ߔࠆᓸ↢‛ࠍᵴᕈൻߒޔല₸⊛ߦ VFA ࠍ↢↥ߐߖࠆߎߣ߇㊀ⷐߣߥࠆޕVFA Ⴎࠍ⋥ធޔ㘺ᢱߦᷝടߒߡਈ߃ࠆߣޔ࡞࡯ࡔࡦ⚦⩶߇ ⎕უߐࠇߡߒ߹߁ߎߣ߇ෂᗋߐࠇࠆߎߣ߆ࠄޔࡊࡠࡃࠗࠝ࠹ࠖࠢࠬࠍ⛎ਈߔࠆߎߣߦࠃࠅޔ ╙৻⢗ౝߢࡊࡠࡇࠝࡦ㉄⊒㉂ࠍଦㅴߐߖࠆᛛⴚ㐿⊒߇㊀ⷐߣߥࠆޕ ╙৻⢗ ᤃ⊒㉂ᕈ὇᳓ൻ‛ ਅㇱᶖൻ▤ ⴊᶧ 㕖䉝䊮䊝䊆䉝ᘒ⓸⚛ ὇᳓ൻ‛ ⓸䇭⚛ 䉝䊮䊝䊆䉝 䊒䊨䊏䉥䊮㉄ 䉫䊦䉮䊷䉴 ᓸ↢‛Ⱞ⊕⾰ ૕Ⱞ⊕⾰ ዩ⚛ 䉝䊚䊉㉄ ዩਛዩ⚛䇭 ឃᴭ㊂ ࿑䋲䇭䊒䊨䊏䉥䊮㉄䈮䉋䉎䉫䊦䉮䊷䉴䈫䌎ઍ⻢೙ᓮ ╙৻⢗ ᤃ⊒㉂ᕈ὇᳓ൻ‛ ਅㇱᶖൻ▤ ⴊᶧ 㕖䉝䊮䊝䊆䉝ᘒ⓸⚛ ὇᳓ൻ‛ ⓸䇭⚛ 䉝䊮䊝䊆䉝 䊒䊨䊏䉥䊮㉄ 䉫䊦䉮䊷䉴 ᓸ↢‛Ⱞ⊕⾰ ૕Ⱞ⊕⾰ ዩ⚛ 䉝䊚䊉㉄ ዩਛዩ⚛䇭 ឃᴭ㊂ ╙৻⢗ ᤃ⊒㉂ᕈ὇᳓ൻ‛ ਅㇱᶖൻ▤ ⴊᶧ 㕖䉝䊮䊝䊆䉝ᘒ⓸⚛ ὇᳓ൻ‛ ⓸䇭⚛ 䉝䊮䊝䊆䉝 䊒䊨䊏䉥䊮㉄ 䊒䊨䊏䉥䊮㉄ 䉫䊦䉮䊷䉴䉫䊦䉮䊷䉴 ᓸ↢‛Ⱞ⊕⾰ ᓸ↢‛Ⱞ⊕⾰ ૕Ⱞ⊕⾰ ૕Ⱞ⊕⾰ ዩ⚛ ዩ⚛ 䉝䊚䊉㉄ 䉝䊚䊉㉄ ዩਛዩ⚛䇭 ឃᴭ㊂ ࿑䋲䇭䊒䊨䊏䉥䊮㉄䈮䉋䉎䉫䊦䉮䊷䉴䈫䌎ઍ⻢೙ᓮ

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㧟㧚ᛛⴚታ↪ൻߩ⃻⁁ ࡅ࠻ࠍ฽߼ߚ෻⧖േ‛એᄖߩູ੃േ‛ߦ߅޿ߡޔVFA ߩ↢ℂ࡮⮎ℂ૞↪ࠍ೑↪ߒߚޔVFA ਥ૕ߩᷝട೷ߩᔕ↪߇කቇߩߺߥࠄߕࡍ࠶࠻↥ᬺߦ߅޿ߡᦼᓙߐࠇߡ޿ࠆޕߒ߆ߒޔߎࠇ߹ ߢޔ࠙ࠪࠍ↪޿ߚ VFA ਥ૕ߩᷝട೷ߩታ↪ൻࠍ⋡⊛ߣߒߚ⎇ⓥߪޔ޽߹ࠅⴕࠊࠇߡ޿ߥ߆ߞ ߚޕߘߩᓟޔVFA ߩ㘺ᢱᷝട೷ߣߒߡߩ⹜㛎߇ⴕࠊࠇࠆࠃ߁ߦߥࠅޔࡈ࡜ࡦࠬߦ߅޿ߡ㈼㉄ Ⴎࠍ↪޿ߚ㘺ᢱᷝട೷߇໡ຠൻߐࠇߡ޿ࠆޕ৻ᣇޔሶ࠙ࠪ↪ࡊ࡟ࡃࠗࠝ࠹ࠖࠢࠬߣߒߡߪޔ ࠝ࡝ࠧ♧ޔ㜞ᶖൻᕈ❫⛽ߦᵈ⋡߇㓸߹ߞߡ߅ࠅޔࡊࡠࡃࠗࠝ࠹ࠖࠢࠬߣߒߡߪޔ੃㉄⩶߇ᵈ ⋡ߐࠇ໡ຠൻߐࠇߡ޿ࠆޕߒ߆ߒޔVFA ⊒㉂ߦ⋥ធ㑐ㅪߔࠆࡊࡠࡃࠗࠝ࠹ࠖࠢࠬߦߟ޿ߡߩ ໡ຠ㐿⊒ߪⴕࠊࠇߡ޿ߥ޿ޕ᣿ᴦ㘺♳ᩣᑼળ␠ߢߪޔ੃⵾ຠ⵾ㅧㆊ⒟ߢᓧࠄࠇࠆ੃㉄⩶⊒㉂ ೽↥‛(LG)ߣࡊࡠࡇࠝࡦ㉄⩶⊒㉂‛(Profec)ߩᷙว‛ࠍሶ࠙ࠪ↪㘺ᢱߣߒߡ೑↪ߔࠆᛛⴚ㐿 ⊒ߦᚑഞߒߚޕߎߩ‛⾰ߪޔູ੃ሶ࠙ࠪߩਅㇱᶖൻ▤ߦ߅޿ߡ᦭↪⩶ߢ޽ࠆࡆࡈࠖ࠼ࡃࠢ࠹ ࡝࠙ࡓߩჇᱺࠍ․⇣⊛ߦଦㅴߒޔ᦭ኂ⚦⩶ߩჇᱺࠍᛥ೙ߔࠆޕ߹ߚޔVFA ߩ↥↢ࠍଦㅴߒޔ ࠕࡦࡕ࠾ࠕߩ↢ᚑࠍᛥ೙ߔࠆޕߐࠄߦޔ♮ଢਛߩ IgA ᛫૕㊂ࠍჇടߐߖࠆߥߤ఺∉ᯏ⢻ࠍ੣ ㅴߐߖޔሶ࠙ࠪߩ⊒⢒ࠍଦㅴߔࠆޕᧄ໡ຠߪޔߔߢߦ⇓↥⃻႐ߦ߅޿ߡ೑↪ߐࠇߡ޿ࠆޕሶ ࠙ࠪߩ㘺ᢱߦᧄ໡ຠࠍᷝടߔࠆߎߣߦࠃࠅޔູ੃ᦼ߿㔌੃ᦼߩᩕ㙃↢ℂቇ⊛ߦਇ቟ቯߥᤨᦼ ߦޔஜᐽࠍ⛽ᜬߒޔ૕㊀ࠍჇᄢߐߖߡ↢↥ᕈࠍ਄ߍࠆߥߤޔ⇓↥ᬺ߳ߩ⽸₂ᐲ߇㕖Ᏹߦᄢ߈ ޿ߣᕁࠊࠇࠆޕ 㧠㧚੹ᓟߩᣇะޔ⺖㗴  ੹ᓟߩ⎇ⓥ⺖㗴ߣߒߡߪޔሶ࠙ࠪߩᶖൻ▤ߢߩ VFA ⊒㉂ࠍ⦟ᅢߦ⛽ᜬߔࠆߚ߼ޔߐࠄߥࠆ 㘺ᢱᷝട‛ߩ㐿⊒ߦะߌߡขࠅ⚵ࠎߢࠁߊޕߎࠇ߹ߢޔᧄຠߪઍ↪੃ߦᷝടߒߡሶ࠙ࠪ߳⛎ ਈߒߡ߅ࠅޔ㔌੃ᓟߩ╙৻⢗ᯏ⢻߇⊒㆐ߒߚሶ࠙ࠪߢߩ೑↪ᕈߦߟ޿ߡߪᬌ⸛ߐࠇߡ޿ߥ޿ޕ ੹ᓟߪޔੱᎿ੃߿ሶ࠙ࠪ↪Ớෘ㘺ᢱߥߤ࿕ᒻ㘺ᢱ߳ߩᷝടߦㆡߒߚ LG ߿ Profec ߩᕈ⁁ࠍᡷ ༀߔࠆߎߣߦࠃࠅޔᧄຠࠍᷝടߒߚߣ߈ߩሶ࠙ࠪߩ╙৻⢗⊒㉂߿ਅㇱᶖൻ▤ߢߩ↢ℂᕈ⁁ߩ ᄌേޔ↢૕ߢߩઍ⻢ᯏ⢻ޔ఺∉ᯏ⢻ߦਈ߃ࠆᓇ㗀ࠍ♖ᩏߒߡޔ㔌੃ᓟߩሶ࠙ࠪߦ߅ߌࠆࡊࡠ ࡃࠗࠝ࠹ࠖࠢࠬߩലᨐࠍ᣿ࠄ߆ߦߒߡࠁߊޕᧄຠߩ㐿⊒ߦࠃࠅޔሶ࠙ࠪߩ៊⠻₸ߩૐਅߣᚑ 㐳ଦㅴ߇ߐࠄߦᦼᓙߢ߈ࠆ߽ߩߣᕁࠊࠇࠆޕ 㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥ౏⴫ႎ๔╬

㧝㧕K. Kitade, et al.㧦Effects of weaning on carbonic anhydrase activity in the palotid gland, rumen and abomasum of Holstein calves. Journal of Comparative Physiology B, 172, 379-385, 2002.

㧞㧕S. Yonekura, et al.㧦Effects of aging and weaning on mRNA expression of leptin and CCK receptors in the calf rumen and abomasum. Domestic Animal Endocrinology, 22, 25-35, 2002.

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ኅ⇓ឃᴭ‛ߩಣℂ࡮ၸ⢈ൻߦ㑐ࠊࠆᓸ↢‛ߣߘߩᵴ↪

㧔᧲ർᄢቇ ᢎ᝼ ਛ੗ ⵨㧕 ᐔᚑ 12㨪14 ᐕᐲᆔ⸤ޟኅ⇓ឃᴭ‛ߩಣℂ෸߮ၸ⢈ൻߦ㑐ࠊࠆᓸ↢‛ߩ⸃᣿ߣߘߩ೑↪ޠ㧔᧲ർᄢቇ ᢎ᝼㧕 㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ ឃᴭ‛ߩಣℂ࡮ၸ⢈ൻߦᄙߊߩᓸ↢‛(ᓸ↢‛⟲㓸)߇㑐ࠊߞߡ޿ࠆߎߣߪᐢߊ⍮ࠄࠇߡ޿ࠆ ߇ޔ⎇ⓥ㐿ᆎᤨ߫߆ࠅߢߥߊޔ⃻࿷߽ߘߩో⽩ߪ᣿⏕ߢߪߥ޿ޕ޿ࠊ߫ޔᓸ↢‛⟲㓸ߣ޿߁ ࡉ࡜࠶ࠢࡏ࠶ࠢࠬࠍᛴ߃ߚ߹߹ޔᳪ᳓ಣℂᣉ⸳߿ၸ⢈ ൻᣉ⸳߇૞ࠄࠇޔ೑↪ߐࠇߡ߈ߚޕ  ⇓↥⃻႐ߦㆡߒߚᳪ᳓ಣℂᴺߣߒߡߩ࡜ࠣ࡯ࡦ߅ࠃ ߮ᵴᕈᳪᵆᴺޔឃᴭ‛ߩ࡝ࠨࠗࠢ࡞ߩ㕙ߢၸ⢈ൻߦᵈ ⋡ߒޔߎࠇࠄߦ㑐ࠊࠆᓸ↢‛ߩ↢ᘒ߿㜞ᯏ⢻ࠍᜬߟᓸ ↢‛ࠍ᣿ࠄ߆ߦߔࠆߎߣޔಽ㔌ᓸ↢‛ࠍ࡜ࠣ࡯ࡦ߅ࠃ ߮ၸ⢈ߦធ⒳ߒߡಣℂല₸ߩะ਄ࠍ⹜ߺࠆߎߣࠍ⋡ ᮡߦ⎇ⓥࠍⴕߞߚޕߣߊߦޔࠕࡦࡕ࠾ࠕߪޔኅ⇓ឃᴭ ‛ಣℂㆊ⒟ߢ⊒↢ߔࠆਥⷐߥᖡ⥇‛⾰ߢ޽ࠅޔ᷷ቶല ᨐࠟࠬߣߒߡ૞↪ߔࠆ਄ߦ㉄ᕈ㔎ߩේ࿃ߣ߽ߥࠅޔࠦࡦࡐࠬ࠻ߢߪࠕࡦࡕ࠾ࠕើᢔߦࠃࠆ⢈ ലᚑಽߢ޽ࠆ⓸⚛ಽߩ៊ᄬ߇໧㗴ߣߥࠆߎߣ߆ࠄޔࠕࡦࡕ࠾ࠕߩើᢔ㒐ᱛ߅ࠃ߮㒰෰ߦ㑐ࠊ ࠆᓸ↢‛ࠍኻ⽎ߦ⎇ⓥࠍታᣉߒߚ(࿑㧝)ޕ  ߘߩ⚿ᨐޔ࡜ࠣ࡯ࡦߦ߅޿ߡߪޔᳪ᳓ಣℂߢ৻⥸ߦ㊀ⷞߐࠇࠆ⎣ൻ⚦⩶߅ࠃ߮⣕⓸⚦⩶ߢ ߪߥߊޔࠕࡦࡕ࠾ࠕหൻᯏ⢻ࠍ߽ߟᓸ↢‛߇⓸⚛㒰෰ߩ㊀ⷐߥᓎഀࠍṶߓࠆߎߣࠍ᣿ࠄ߆ߦ ߒߚޕߘߩᓟޔ㜞޿ࠕࡦࡕ࠾ࠕหൻ⢻ࠍᜬߟ⚦⩶ᩣࠍᄙᢙಽ㔌ߒޔߘࠇࠄߩ޿ߊߟ߆ߪޔ࡜ ࠣ࡯ࡦߢఝභ⊛ߥ⒳ߢ޽ࠆߎߣࠍㆮવሶ⸃ᨆᴺߦࠃࠅ⏕⹺ߒߚޕߒߚ߇ߞߡޔ࡜ࠣ࡯ࡦߢߪ ᦭ᯏᘒ⓸⚛ߪᓸ↢‛⚦⢩᭴ᚑᚑಽߦᄌ឵ߐࠇޔᓸ↢‛૕߇ࠬ࡜࠶ࠫߣߒߡᴉᲚߔࠆߎߣߦࠃ ࠅᳪ᳓ߩᵺൻ߇ⴕࠊࠇߡ޿ࠆߣ⚿⺰ߐࠇߚޕ  ࠦࡦࡐࠬ࠻߆ࠄޔࠕࡦࡕ࠾ࠕࠍ⓸⚛Ḯߣߒߡ೑↪ߢ߈ޔߒ ߆߽㜞Ớᐲߩࠕࡦࡕ࠾ࠕߦ⠴ᕈࠍ᦭ߔࠆᓸ↢‛ࠍޔ37͠߅ࠃ ߮ 55͠ߩၭ㙃᷷ᐲߢಽ㔌ߒߚޕߘߩਛߩ㧞ᩣߩ᡼✢⩶ߪޔㆮ વሶ⸃ᨆߦࠃࠅࠦࡦࡐࠬ࠻ൻㆊ⒟ߦ߅ߌࠆఝභ⒳ߢ޽ࠆߎ ߣ߇᣿ࠄ߆ߣߥࠅޔࠦࡦࡐࠬ࠻ਛߢఝභ⊛ߦჇᱺߢ߈ࠆน⢻ ᕈ߇␜ߐࠇߚ(࿑㧞)ޕ 㧞㧚⎇ⓥ೔㆐ߩ⃻⁁  ಽ㔌ᓸ↢‛ᩣߪޔኅ⇓ឃᴭ‛ಣℂㆊ⒟ߦሽ࿷ߔࠆᓸ↢‛⟲㓸ࠍᷙวߒߡၭ㙃ߒߡ߽ޔ㜞޿ ࠕࡦࡕ࠾ࠕหൻ⢻ࠍ⊒ើߒޔ࡜ࠣ࡯ࡦ߅ࠃ߮ࠦࡦࡐࠬ࠻ౝߦ߅޿ߡ߽ߘߩᯏ⢻ࠍ␜ߔߣ⠨߃ ࠄࠇߚޕ߹ߚޔᓸ↢‛ࠍၒ߼ㄟࠎߛ᮸⢽ࡆ࡯࠭ࠍ૞ᚑߒޔߎࠇߦࠃࠅࠕࡦࡕ࠾ࠕࠍ㒰෰ߢ߈ ࠆߎߣࠍ᣿ࠄ߆ߦߒߚޕߎࠇࠄߩߎߣ߆ࠄޔ࡜ࠣ࡯ࡦ߅ࠃ߮ࠦࡦࡐࠬ࠻ߦ߅ߌࠆࠕࡦࡕ࠾ࠕ หൻ⚦⩶ߩ㊀ⷐᕈ߇᣿⏕ߦߥࠅޔᓸ↢‛ߩࠕࡦࡕ࠾ࠕหൻࠍะ਄ߐߖࠆᣉ⸳ߩ⸳⸘߿Ⓙ௛ᣇ ᴺߩᡷ⦟ޔಽ㔌ᩣߩ೑↪ߥߤታ↪ൻߦะߌߡ㐿⊒߇⛯ߌࠄࠇߡ޿ࠆޕ

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 ᧄ⎇ⓥߦࠃߞߡޔⅣႺᓸ↢‛⟲㓸⸃ᨆᚻᴺߩၮ␆߇૞ࠄࠇߚ߇ޔߐࠄߦㆮવሶ⸃ᨆᛛⴚࠍ ะ਄ߐߖޔᐢ▸࿐ߩᓸ↢‛⟲ߩᖱႎࠍᓧߚޕ⃻࿷ޔᓸ↢‛⟲㓸᭴ㅧߦ㑐ߔࠆ⍮⷗ࠍ㓸Ⓧਛߢ ޽ࠅޔߎࠇࠄߩ⍮⷗ߪޔኅ⇓ឃᴭ‛ಣℂᣉ⸳ߩᣂⷙ㐿⊒߅ࠃ߮⛽ᜬ▤ℂߦᵴ↪ߢ߈ࠆޕࠦࡦ ࡐࠬ࠻ߦ㑐ߒߡߪޔᣉ⸳Ფߩᓸ↢‛⟲㓸ߩ㆑޿߿ࠦࡦࡐࠬ࠻ൻㆊ⒟ߦ߅ߌࠆᓸ↢‛⟲㓸᭴ㅧ ߩᄌൻ߇⹦⚦ߦ␜ߐࠇߟߟ޽ࠅޔᣂⷙߩࠕࡦࡕ࠾ࠕ㉄ൻฎ⚦⩶ߩ⎇ⓥ߇ዷ㐿ߐࠇߡ޿ࠆޕࠕ ࡦࡕ࠾ࠕหൻ⢻ࠍ߽ߟᓸ↢‛ߪޔ⇓↥⨲࿾⎇ⓥᚲߩ㤥↰ࠄߦࠃߞߡ․⸵⊓㍳ߐࠇޔ⣕⥇ᯏ⢻ ߦᵈ⋡ߒߚታ↪ൻ߇ㅴ߼ࠄࠇߡ޿ࠆޕߒ߆ߒޔᒰ⹥ಽ㊁ߦ߅ߌࠆ⎇ⓥ⠪ߪ㒢ࠄࠇޔ⎇ⓥߩㅴ ዷߪㆃߊޔ⧯ᚻ⎇ⓥ⠪ߩ⢒ᚑߩߚ߼ߦ߽ޔ⎇ⓥᡰេ੐ᬺߪ㊀ⷐߢ޽ࠆޕ  ߥ߅ޔᧄ⎇ⓥቶߢߪޔᧄᆔ⸤੐ᬺߩᡰេߦࠃࠅޔኅ⇓ឃᴭ‛ಣℂ࡮࡝ࠨࠗࠢ࡞ߦ㑐ߔࠆᓸ ↢‛ߦ㑐ߔࠆ⎇ⓥ߇ᄢ޿ߦ⊒ዷߒޔ⎇ⓥઍ⴫⠪ߩ╙㧥࿁਎⇇⇓↥ળ⼏ᦨఝ⑲⊒⴫⾨(2003 ᐕ)ޔ ⎇ⓥᜂᒰቇ↢ߩ╙ 103 ࿁ᣣᧄ⇓↥ቇળᄢળఝ⑲⊒⴫⾨(2004 ᐕ)ޔ᧲ർᄢቇᄢቇ㒮ㄘቇ⎇ⓥ⑼ 㐳⾨(2004 ᐕ)ޔBest Poster Award, International symposium on Microbial Ecology, Asia

2007ฃ⾨ߥߤߩᒻߢޔ࿖ౝᄖߢ㜞ߊ⹏ଔߐࠇߡ޿ࠆޕ 㧟㧚ᛛⴚታ↪ൻߩ⃻⁁  ࠕࡦࡕ࠾ࠕߦ㑐ߒߡߪޔหൻߦട߃ޔࠕࡦࡕ࠾ࠕ㉄ൻߦ㑐ࠊࠆ⚦⩶⟲㓸᭴ㅧ߇᣿⏕ߦߐࠇ ߟߟ޽ࠅޔߘߩᖱႎࠍᵴᕈᳪᵆᴺߩⒿ௛⁁ᴫߩࡕ࠾࠲࡝ࡦࠣ߿ᣂⷙᣉ⸳ߩ┙ߜ਄ߍߦ↪޿ࠆ ߎߣ߇ታ↪Ბ㓏ߦㄭߠ޿ߡ޿ࠆޕ߹ߚޔߎࠇࠄߩ⎇ⓥߪޔᣂⷙߩህ᳇ᕈࠕࡦࡕ࠾ࠕ㉄ൻ⚦⩶ ߩ⸃ᨆߣᔕ↪⎇ⓥߦᵴ↪ߐࠇߡ޿ࠆޕߎࠇࠄߩ⍮⷗߅ࠃ߮ᛛⴚߪޔ⇓↥ᬺ߳ߩ⽸₂߇ᦼᓙߐ ࠇࠆߣߣ߽ߦޔⅣႺቇ߿࿯ᧁᎿቇಽ㊁߳ߩᵄ෸ലᨐ߇ᄢ߈޿ߣᕁࠊࠇࠆޕ 㧠㧚੹ᓟߩᣇะޔ⺖㗴  ኅ⇓ឃᴭ‛ಣℂ߅ࠃ߮೑↪ߪ੹ᓟ߽㊀ⷐߥ໧㗴ߢ޽ࠆޕኅ⇓ឃᴭ‛ߦߪޔᓥ᧪ߩㇺᏒਅ᳓ ಣℂ߿ᵺൻᮏޔ↢ߏߺࠦࡦࡐࠬ࠻ൻߥߤߦ㑐ߔࠆ⍮⷗߿ᛛⴚࠍ⋥ធᒰߡߪ߼ࠄࠇߥ޿ߎߣ߇ ⚻㛎ߐࠇߡ߅ࠅޔኅ⇓ឃᴭ‛ࠍኻ⽎ߣߔࠆ⁛⥄ߩ⎇ⓥ߅ࠃ߮ታ↪ᛛⴚ㐿⊒߇ᔅⷐߢ޽ࠆޕ⇓ ↥ಽ㊁ߩᛛⴚߦߪૐࠦࠬ࠻߇ᒝߊ᳞߼ࠄࠇࠆߎߣ߆ࠄޔߘߩታ⃻ߦߪ㐿⊒⾌ߩᄖㇱេഥ߇ਇ นᰳߣߥࠆޕ⎇ⓥౝኈߛߌߢߪߥߊ⚻ᷣ㕙ߦ߅޿ߡ߽⇓↥ߣઁಽ㊁ߣߩ㆑޿ߪᄢ߈ߊޔߣߊ ߦ⇓↥ⅣႺಽ㊁ߦኻߔࠆ㐿⊒⾗㊄ᡰេ߇⛮⛯ߐࠇࠆߎߣࠍᒝߊᦸ߻ޕ 㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥ౏⴫ႎ๔╬

㧝㧕Otawa K., Asano R., Nakai Y., et al.㧦Molecular analysis of ammonia-oxidizing bacteria community in intermittent aeration sequencing batch reactors used for animal wastewater treatment. Environ. Microbiol., 8(11), 1985-1996, 2006.

2㧕 Sasaki H., Yano H., Sasaki T., Nakai Y. 㧦 A survey of ammonia-assimilating micro-organisms in cattle manure composting. J. Appl. Microbiol., 99, 1356-1363, 2005.

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૕⚦⢩ࠢࡠ࡯ࡦኅ⇓ߩ૞಴ߦ઻߁ㆮવ⊛቟ోᕈߩ⹏ଔ

㧔ጟጊᄢቇ ᢎ᝼ ࿖ᨑືᄦ㧕 ᐔᚑ 13㨪15 ᐕᐲᆔ⸤ޟ૕⚦⢩ࠢࡠ࡯ࡦേ‛ߩㆮવ⊛቟ోᕈߩ⹏ଔߦ㑐ߔࠆ⎇ⓥޠ㧔ጟጊᄢቇ ᢎ᝼㧕 㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥౝኈ 1996ᐕߦࡅ࠷ࠫߦ߅޿ߡᩭ⒖ᬀߦࠃࠆ૕⚦⢩ࠢࡠ࡯ࡦ୘૕ߩ૞಴߇ႎ๔ߐࠇߡએ᧪ޔᄙߊ ߩേ‛ߢ૕⚦⢩ࠢࡠ࡯ࡦ߇૞ᚑߐࠇޔኅ⇓↢↥߳ߩᔕ↪߽ᬌ⸛ߐࠇߡ޿ࠆޕߒ߆ߒޔߎࠇ߹ ߢߦ߽ࠢࡠ࡯ࡦ⢦ߢߪޔㆊᄢ੿߿⇣Ᏹ୘૕ߩ಴⃻ߥߤᱜᏱߥ⢦⊒↢߇࿎㔍ߥ႐ว߇޽ࠆߎߣ ߇ႎ๔ߐࠇޔߎࠇࠄߩ⢦⊒↢⇣Ᏹߪޔ૕⚦⢩ߩࠥࡁࡓ⥄りߦ୘૕ߩᱜᏱߥ⊒↢ࠍ࿎㔍ߣߔࠆ ⓭ὼᄌ⇣߇↢ߓߡ޿ࠆน⢻ᕈ߽⠨߃ࠄࠇࠆޕࠢࡠ࡯ࡦേ‛ߦ߅޿ߡ․ቯߩㆮવሶߦ⓭ὼᄌ⇣ ߇↢ߓߡ޿ࠆ႐วߪޔߘߩᄌ⇣߇ࠢࡠ࡯ࡦ୘૕↱᧪ߩᰴ਎ઍ߳વ㆐ߒޔߐࠄߦሶቊ߇ኅ⇓ߩ ↢↥㓸࿅ਛߦᐢߊ᜛ᢔߔࠆน⢻ᕈ߇޽ࠆߚ߼ޔߘߩᓇ㗀ߪ㊀ᄢߢ޽ࠈ߁ޕߒߚ߇ߞߡޔࠢࡠ ࡯ࡦേ‛ࠍኅ⇓↢↥ߦᔕ↪ߔࠆߦ޽ߚߞߡߪޔߎߩࠃ߁ߥ⓭ὼᄌ⇣⊒↢ߩน⢻ᕈࠍ෩ኒߦᬌ ⸽ߒޔߘߩㆮવ⊛ߥ቟ోᕈࠍ⹏ଔޔ⸽᣿ߔࠆߎߣ߇ਇนᰳߢ޽ࠆޕߘߎߢޔᧄ⎇ⓥߢߪࡑ࠙ ࠬߩ૕⚦⢩ࠢࡠ࡯ࡦࠍ↪޿ޔᄢⷙᮨߥ੤㈩ታ㛎ߣోࠥࡁࡓࠍ✂⟜ߔࠆ⸃ᨆߦࠃࠅޔࠢࡠ࡯ࡦ ૞಴ㆊ⒟ߢߩ⓭ὼᄌ⇣⊒↢ߩน⢻ᕈߦߟ޿ߡᬌ⸛ߔࠆߎߣࠍ⋡⊛ߣߒߚޕ 㧞㧚⎇ⓥ೔㆐ߩ⃻⁁ ᧄ⎇ⓥߢߪޔ࠮࡞࠻࡝⚦⢩ޔෆਐ⚦⢩ޔ❫⛽⧘⚦⢩ߩᩭࠍᧂฃ♖ෆߦ⒖ᬀߔࠆߎߣߢ૕⚦ ⢩ࠢࡠ࡯ࡦࡑ࠙ࠬࠍ૞ᚑߒޔߎࠇࠄߩࡑ࠙ࠬߦ߅ߌࠆㆮવ⊛ᄌ⇣ࠍᬌ಴ߔࠆߎߣࠍ⹜ߺߚޕ ߹ߕޔߎࠇࠄߩࠢࡠ࡯ࡦ୘૕ࠍ㐳ᦼ㘺⢒ߒޔࠢࡠ࡯ࡦ୘૕⥄りߦ⇣Ᏹߣߒߡ⃻ࠇࠆᄌ⇣߇ ሽ࿷ߒߡ޿ߥ޿߆ࠍ⺞ߴࠆߣߣ߽ߦޔࠢࡠ࡯ࡦ୘૕ߩࠥࡁࡓ਄ߦ࡟࠻ࡠ࠻࡜ࡦࠬࡐ࠱ࡦߩᝌ ౉ߥߤߩㆮવ⊛ᄌൻ߇↢ߓߡ޿ࠆน⢻ᕈߦߟ޿ߡᬌ⸛ߒߚޕᰴߦޔࠢࡠ࡯ࡦ୘૕ߣㄭ੤♽ࡑ ࠙ࠬߣߩ੤㈩ߦࠃࠅᓧߚޔᄙᢙߩ F2 ޽ࠆ޿ߪᚯߒ੤㈩୘૕ߦߟ޿ߡޔࠢࡠ࡯ࡦ୘૕↱᧪ߩߔ ߴߡߩᨴ⦡૕਄ߩ㗔ၞ߇વ㆐ߒߡ޿ࠆ߆ࠍޔ✂⟜⊛ߥࡑ࡯ࠞ࡯ߩㆮવሶဳ್ቯߦࠃࠅᬌ಴ߔ ࠆߎߣࠍ⹜ߺߚޕߎߩᣇᴺߦࠃࠅޔࠢࡠ࡯ࡦ୘૕⥄りߦߪ಴⃻ߒߥ޿ഠᕈߩ⓭ὼᄌ⇣߇ᬌ಴ น⢻ߢ޽ࠆߣߣ߽ߦޔᓥ᧪ߪᬌ಴࿎㔍ߣߐࠇߚ⢝ ↢⥌ᱫ߿⊒↢஗ᱛࠍᒁ߈⿠ߎߔᄌ⇣ߦߟ޿ߡ߽ޔ ోߡᬌ಴ߔࠆߎߣ߇น⢻ߢ޽ࠆޕ ߘߩ⚿ᨐޔ৻ㇱߩࠢࡠ࡯ࡦേ‛ߢߪ♖ሶᒻᚑ⇣ Ᏹߦࠃࠆਇᅧ(࿑㧝)ޔ⣛⤳߅ࠃ߮࡝ࡦࡄ⣼ߦ߅ߌ ࠆ⣲≌ߩ⊒↢(࿑㧞)߇⏕⹺ߐࠇߚޕߎࠇࠄߩ⇣Ᏹ ߩ⊒↢߇ࠢࡠ࡯ࡦࡑ࠙ࠬߩࠥࡁࡓ਄ߦ↢ߓߚㆮ વ⊛ᄌൻߦ⿠࿃ߔࠆߩ߆ޔࠢࡠ࡯ࡦ୘૕ߦ৻⥸⊛ ߦ⹺߼ࠄࠇࠆࠛࡇࠫࠚࡀ࠹ࠖ࠶ࠢߥᄌൻߦ⿠࿃ ߔࠆߩ߆ߪ⃻ᤨὐߢߪਇ᣿ߢ޽ࠆޕߐࠄߦޔ࡟࠻ ࡠ࠻࡜ࡦࠬࡐ࠱ࡦߩᝌ౉ࠍᬌ಴ߔࠆ S-SAP ᴺߦ ࠃࠅᓧࠄࠇߚ㔚᳇ᵒേ௝ࠍᲧセߒߚߣߎࠈޔ․ቯ ߩࠢࡠ࡯ࡦ୘૕ߢઁߩ୘૕ߣ⇣ߥࠆࡃࡦ࠼߇⏕ ⹺ߐࠇߚߎߣ߆ࠄ(࿑㧟)ޔࠢࡠ࡯ࡦࡑ࠙ࠬ૞಴ㆊ⒟ߢ࡟࠻ࡠ࠻࡜ࡦࠬࡐ࠱ࡦ߇ᣂߚߦ⒖േߒޔ ࿑㧝 ࠮࡞࠻࡝⚦⢩ߦ↱᧪ߔࠆ㓶ߩࠢࡠ࡯ ࡦ୘૕ߩ߁ߜ 2 ୘૕߇ਇᅧߢ޽ࠅޔ⚵❱ቇ ⊛⸃ᨆߦࠃࠅޔ♖ሶᒻᚑ߇㒖ኂߐࠇߡ޿ࠆ ߎߣ߇᣿ࠄ߆ߣߥߞߚޕ

A B

(19)

ࠥࡁࡓਛߩઁߩ૏⟎ߦᝌ౉ߐࠇߡ޿ࠆน⢻ᕈ߇␜ໂߐ ࠇߚޕ৻ᣇޔ✢⛽⧘⚦⢩↱᧪ߩ૕⚦⢩ࠢࡠ࡯ࡦ߆ࠄᓧ ࠄࠇߚሶቊߦ․ቯߩ⇣Ᏹߪ⹺߼ࠄࠇߕޔ߹ߚޔోᨴ⦡ ૕ࠍ✂⟜ߔࠆࡑࠗࠢࡠࠨ࠹࡜ࠗ࠻ࡑ࡯ࠞ࡯ߩㆮવሶဳ ್೎ߦࠃࠅޔߔߴߡߩᨴ⦡૕ߩ㗔ၞߦߟ޿ߡࡎࡕធว ߣߥߞߡ޿ࠆ୘૕߇ᓧࠄࠇߚߎߣ߆ࠄޔߎࠇࠄߩࠢࡠ ࡯ࡦࡑ࠙ࠬߦߪ⢝↢⥌ᱫࠍ฽߼ߚഠᕈߩ⓭ὼᄌ⇣ߪሽ ࿷ߒߥ޿ߎߣ߇᣿ࠄ߆ߣߥߞߚޕ 㧟㧚ᛛⴚታ↪ൻߩ⃻⁁  ᧄ⎇ⓥߩ⚿ᨐޔ૕⚦⢩ࠢࡠ࡯ࡦ ࡑ࠙ࠬߢ♖ሶᒻᚑ⇣Ᏹޔ⣲≌ߩ⊒ ↢߇⏕⹺ߐࠇޔߎࠇࠄߩ⇣Ᏹߩ⊒ ↢₸߇ࠢࡠ࡯ࡦ୘૕ߢ਄᣹ߒߡ ޿ࠆน⢻ᕈ߽⠨߃ࠄࠇߚޕ߹ߚޔ ࠢࡠ࡯ࡦ૞಴ߩㆊ⒟ߢޔ࡟࠻ࡠ࠻ ࡜ࡦࠬࡐ࠱ࡦ╬ߩォ⒖ᕈ࿃ሶ߇ ᵴᕈൻߒߡ޿ࠆน⢻ᕈ߇␜ໂߐ ࠇߚޕߒߚ߇ߞߡޔኅ⇓ߦ߅޿ߡࠢࡠ࡯ࡦ୘૕ࠍ૞಴ߔࠆ਄ߢߪߎࠇࠄߩ ⇣Ᏹ߇⊒↢ߔࠆ߆ߤ߁߆ߦߟ޿ߡචಽߦᵈᗧߔߴ߈ߢ޽ࠆߣ⠨߃ࠄࠇߚޕ ৻ᣇߢޔࠢࡠ࡯ࡦ୘૕ߦ↱᧪ߔࠆሶቊߦ⴫⃻ဳߦ⃻ࠇࠆ⇣Ᏹߪ಴⃻ߒߡ߅ ࠄߕޔߘߩ߁ߜߩ৻ㇱߦߟ޿ߡߪࠥࡁࡓో૕ࠍ✂⟜ߔࠆ⸃ᨆߦࠃࠅޔഠᕈ ⥌ᱫߩ⓭ὼᄌ⇣ߪ↢ߓߡ޿ߥ޿ߎߣ߇್᣿ߒߚޕએ਄ߩ⍮⷗ߪ૕⚦⢩ࠢࡠ ࡯ࡦᛛⴚߩ⇓↥ᬺ߳ߩᔕ↪ߦ޽ߚࠅ᦭↪ߣ⠨߃ࠄࠇߚޕ 㧠㧚੹ᓟߩᣇะޔ⺖㗴 એ਄ࠃࠅޔ੹ᓟޔኅ⇓ߦ߅ߌࠆ૕⚦⢩ࠢࡠ࡯ࡦേ‛ߩታ↪ൻߦ㓙ߒߡߪޔ ㈩஧ሶᒻᚑߩ⇣Ᏹ߿ޔ㐳ᦼ㘺⢒ߦࠃࠅ⣲≌╬ߩ⊒↢₸߇Ⴧടߒߡ޿ߥ޿߆ ߣ޿߁ὐߣߣ߽ߦޔ࡟࠻ࡠ࠻࡜ࡦࠬࡐ࠱ࡦ╬ߩォ⒖ᕈ࿃ሶߩᵴᕈൻ߇↢ߓ ߡ޿ߥ޿߆ߤ߁߆ࠍ⺞ߴࠆߎߣ߇ᔅⷐߣ⠨߃ࠄࠇߚޕ߹ߚޔᧄ⎇ⓥߦ߅޿ ߡޔ♖ሶᒻᚑ⇣Ᏹ߿⣲≌ߩ⊒↢ߒߚ୘૕ߪዋᢙߢ޽ࠅޔߎࠇࠄߩ⇣Ᏹߩ⊒ ↢₸߇⛔⸘ቇ⊛ߦ᦭ᗧߦ਄᣹ߒߡ޿ࠆߣߪ⚿⺰ߠߌࠄࠇߥ޿ߎߣ߆ࠄޔ⛮ ⛯ߒߡࠢࡠ࡯ࡦࡑ࠙ࠬߦ߅ߌࠆ⇣Ᏹߩ⊒↢㗫ᐲߦߟ޿ߡ⺞ߴߡ޿ߊߎߣ ߇ᔅⷐߣ⠨߃ࠄࠇߚޕ ࿑㧞 ࠢࡠ࡯ࡦࡑ࠙ࠬߩ⣛⤳(㧭)߅ ࠃ߮㗶ਅ࡝ࡦࡄ▵(㧮)ߦ⹺߼ࠄࠇߚ ⣲≌⚵❱ޕ޿ߕࠇ߽๟࿐ߩ⚵❱ߦᶐ Ảߒߡ޿ࠆޕ ࿑㧟 S-SAP ᴺߦࠃ ࠅ⹺߼ࠄࠇߚ IAP ࡟ ࠻ࡠ࠻࡜ࡦࠬࡐ࠱ࡦ ߩᣂⷙᝌ౉ߣᕁࠊࠇ ࠆࡃࡦ࠼(⍫ශ)ޕ

A

B

(20)

ࡉ࠲⢦ಓ⚿଻ሽߩᛛⴚ㕟ᣂ

㧔᣿ᴦᄢቇㄘቇㇱ ᢎ᝼ 㐳᎑Ყํᔒ㧕 ᐔᚑ 14㨪15 ᐕᐲᆔ⸤ޟታ↪⊛ࡉ࠲⢦ಓ⚿଻ሽᴺߩ⏕┙ޠ㧔᣿ᴦᄢቇ ഥᢎ᝼㧕 㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ ࠙ࠪ⢦ߩಓ⚿଻ሽᛛⴚ߇ታ↪⊛Ბ㓏ߦ㆐ߒߚᓟ߽ޔࡉ࠲⢦ߩಓ⚿଻ሽߪ࿎㔍ߣߐࠇޔߘߩ ߚ߼ታ㓙ߩࡉ࠲ߩ❥ᱺᛛⴚߣߒߡ೑↪ߐࠇߡߎߥ߆ߞߚޕᚒޘߩၮ␆⎇ⓥߦࠃߞߡޔࡉ࠲⢦ ߩಓ⚿଻ሽߩน⢻ᕈ߇␜ߐࠇߚߎߣࠍฃߌޔᧄ⎇ⓥߢߪޔ⒖ᬀ⹜㛎ߦࠃࠆታ↪ᕈߩታ⸽ࠍⴕ ߞߚޕߐࠄߦޔ૕⚦⢩ࠢࡠ࡯࠾ࡦࠣ╬ߩవ┵⊛ᛛⴚߦኻᔕߔࠆߚ߼ޔ૕ᄖᚑᾫෆߦ↱᧪ߔࠆ ࡉ࠲⢦ޔߔߥࠊߜ૕ᄖ↢↥⢦ߩಓ⚿଻ሽᛛⴚߩ㐿⊒ߦ߽ขࠅ⚵ࠎߛޕ  ࡉ࠲᪀ታ⢦ࠍࠟ࡜ࠬൻᴺ(ᦨዊኈ㊂ᴺ)ߢ଻ሽߔࠆߎߣߦࠃࠅޔ㕖Ᏹߦ㜞޿↢ሽᕈ߇ᓧࠄࠇࠆ ߎߣ߇ታ⸽ߐࠇߚޕታᣉߒߚࠟ࡜ࠬൻ⢦ߩ⒖ᬀ⹜㛎ߢߪޔᅧᆼ₸ߪ 100㧑ߢ޽ߞߚޕ૕ᄖ↢ ↥⢦ޔ․ߦࠢࡠ࡯ࡦ⢦߳ߩᔕ↪ࠍ⋡⊛ߣߒߡޔ૕ᄖᚑᾫ↱᧪ߩ㧠⚦⢩ᦼ⢦(නὑ⊒↢)ߩಓ⚿଻ ሽߦขࠅ⚵ࠎߛ⚿ᨐޔ⢦ߦ⚦⢩⾰⢽⢌㗰☸㒰෰ಣ⟎(delipation)ࠍᣉߒߚᓟޔᦨዊኈ㊂ᴺߢࠟ ࡜ࠬൻ଻ሽࠍⴕ߁ߎߣߢޔ㕖ࠟ࡜ࠬൻ⢦ߦ඘ᢜߔࠆ↢ሽ₸߇ᓧࠄࠇࠆߎߣ߇᣿ࠄ߆ߣߥߞߚޕ  ߐࠄߦޔ૕ᄖ↢↥⢦ߩࠟ࡜ࠬൻ଻ሽߩታ↪ᕈะ਄ߦ߽ขࠅ⚵ࠎߛޕ᪀ታ⢦ߩㅘ᣿Ꮺࠍ࠻࡝ ࡊࠪࡦಣℂߦࠃߞߡ⤘ൻߐߖߚᓟߦ㆙ᔃಣℂߔࠆߣޔ⚦⢩⾰⢽⢌㗰☸ࠍ⚦⢩ᄖߦ⒖േߐߖࠆ ߎߣ߇ߢ߈ࠆߎߣࠍ⷗಴ߒޔߎࠇࠍ㕖ଚⷅ⊛ delipation(⢽⢌㗰☸㒰෰ᴺ)ߣߒߡ⏕┙ߒߚޕ㕖 ଚⷅ⊛ delipation ࠍᣉߒߚ૕ᄖᚑᾫ࡮ฃ♖᪀ታ⢦ߩࠟ࡜ࠬൻᓟߩ↢ሽᕈߪޔ㕖ࠟ࡜ࠬൻ⢦ߦ ඘ᢜߔࠆߎߣࠍ⸽᣿ߒߚޕߎߩᣇᴺࠍࠢࡠ࡯ࡦ⢦ߦ߽ᔕ↪ߒޔ૕ᄖᚑᾫ↱᧪ࡉ࠲ࠢࡠ࡯ࡦ⢦ ߩಓ⚿଻ሽߢޔ਎⇇ೋߩᚑഞ଀ࠍᓧߚޕ 㧞㧚⎇ⓥ೔㆐ߩ⃻⁁ ਄⸥ߩ⎇ⓥࠍߐࠄߦ⊒ዷߐߖޔࡉ࠲૕ᄖ↢↥⢦ߩࠟ࡜ࠬൻ଻ሽᓟߩ⒖ᬀߦࠃࠆᱜᏱ↥੿ߩ ૞಴ߦ߽ᚑഞߒߚޕಓ⚿଻ሽߒߚࡉ࠲૕ᄖᚑᾫ࡮ฃ♖⢦߆ࠄߩ↥੿૞಴ߣߒߡޔ਎⇇ೋߩᚑ ഞ଀߇ᓧࠄࠇߚߎߣߪޔ․╩ߦ୯ߔࠆߢ޽ࠈ߁ޕട߃ߡޔࠟ࡜ࠬൻ଻ሽߒߚࡉ࠲ᩭ⒖ᬀ⢦߆ ࠄߩࠢࡠ࡯ࡦࡉ࠲ߩ↢↥ߦ߽ᚑഞߒߚޕએ਄ߩᚑᨐߪޔࡉ࠲૕ౝ↢↥⢦ߛߌߢߥߊޔ૕ᄖ↢ ↥⢦ߩಓ⚿଻ሽߦ߅޿ߡ߽ޔdelipation ߣᦨዊኈ㊂ࠟ࡜ࠬൻᴺߩ⚵ߺวࠊߖ߇㕖Ᏹߦലᨐ⊛ ߢ޽ࠆߎߣࠍ␜ߒߚ߽ߩߢ޽ࠆޕߎߩᣇᴺߩ᦭ലᕈߪޔ਎⇇ߩᄙߊߩ⎇ⓥ⠪ߦࠃࠆㅊ⹜ߦࠃ ߞߡ⏕⹺ߐࠇߡ޿ࠆޕ ࿑ 1 ࡉ࠲૕ᄖ↢↥᪀ታ⢦ߩ㕖ଚⷅ⊛ delipation(⢽⢌㗰☸㒰෰)ಣ⟎ (a)ಣ⟎೨ߩ᪀ታᦼ⢦ޔ(b)࠻࡝ࡊࠪࡦಣℂߦࠃࠆㅘ᣿Ꮺ⤘ൻᓟޔ(c)㆙ᔃಣℂ⋥ᓟߦ⚦⢩⾰⢽⢌㗰☸߇⒖േߒߚ⁁ᘒ

a

b

c

(21)

㧟㧚ᛛⴚታ↪ൻߩ⃻⁁ ࡉ࠲૕ౝ↢↥⢦ߩࠟ࡜ࠬൻ଻ሽᓟߩ↢ሽᕈߪޔ㕖ಓ⚿⢦ߣห╬ߩ࡟ࡌ࡞ߦ㆐ߒߚޕᓥߞߡޔ ⢦ߩಓ⚿଻ሽࠍታ↪⊛ᛛⴚߣߒߡޔࡉ࠲ߩ❥ᱺ߿♽⛔଻ሽߦ೑↪ߔࠆၮ⋚ߪ᭴▽ߐࠇߚߣ޿ ߃ࠃ߁ޕ 㧠㧚੹ᓟߩᣇะޔ⺖㗴 ࡉ࠲⢦ߩಓ⚿଻ሽᛛⴚߪޔ⃻⁁ߩ࡟ࡌ࡞ߢ߽ޔේ⒳⽋߿♽⛔ㅧᚑ⽋ߩㆮવሶ⾗Ḯߩ଻ሽߦ ᄢ߈ߊ⽸₂ߒᓧࠆߢ޽ࠈ߁ޕߚߛߒޔታ↪⊛ᛛⴚߣߒߡ㜞ᐲ೑↪ߔࠆߚ߼ߦߪޔ㕖ᄖ⑼⊛⢦ ⒖ᬀᴺߩ⏕┙ߥߤޔ⢦⒖ᬀᛛⴚߩല₸ൻ߿◲ଢൻ߳ߩขࠅ⚵ߺ߇㊀ⷐߢ޽ࠆޕ  ૕ᄖ↢↥⢦ߦଐሽߔࠆࡉ࠲ߩ❥ᱺᛛⴚߪޔࠢࡠ࡯ࡦߦઍ⴫ߐࠇࠆࠃ߁ߥޔ⎇ⓥ⋡⊛ߦ೑↪ ߐࠇࠆߎߣ߇ᄙ޿ޕ૕ᄖ↢↥⢦ߩಓ⚿଻ሽߦߪޔ⃻⁁ߢߪ㕖ଚⷅ⊛ delipation ಣℂ߇ᔅⷐߢ ޽ࠆޕߘߩࠃ߁ߥಣ⟎ߦ઻߁ᾘ㔀ߐࠍシᷫߒޔࠃࠅታ↪ᕈߩ㜞޿ࠪࠬ࠹ࡓࠍ᭴▽ߔࠆߎߣ߽ޔ ੹ᓟߩ⺖㗴ߢ޽ࠈ߁ޕߚߛߒޔ⎇ⓥᔕ↪ߦ߅޿ߡߪޔ޽ࠆ⒟ᐲߩᛛⴚ⊛ⶄ㔀ߐߪ⸵ኈߐࠇࠆ ஥㕙߽޽ࠅޔ㒢ቯ⊛ߢߪ޽ࠆ߇ޔ૕ᄖ↢↥⢦ߩࠟ࡜ࠬൻ଻ሽᛛⴚߩታ↪⊛೑↪߽น⢻ߢ޽ࠆޕ  ࡉ࠲ߪ⡺↪ኅ⇓ߣߒߡߩ㊀ⷐᕈߦട߃ߡޔㄭᐕޔᄢဳታ㛎േ‛ߣߒߡᄙ↪ߐࠇࠆ௑ะߦ޽ ࠆޕ⢦ߩಓ⚿଻ሽᛛⴚߛߌߢߥߊޔ᭽ޘߥ❥ᱺᛛⴚߩ㐿⊒ޔ㜞ᐲൻ߇੹ᓟ߹ߔ߹ߔ㊀ⷐߣߥ ࠈ߁ޕ  ࿑ 2 ࠟ࡜ࠬൻ଻ሽߐࠇߚࡉ࠲૕ᄖ↢↥⢦ߩ↢ሽᕈ (a)ࠟ࡜ࠬൻ଻ሽߐࠇߚ૕ᄖᚑᾫ࡮ฃ♖⢦㧔4-8⚦⢩ᦼ㧕ߩ⒖ᬀߢᓧࠄࠇߚሶ⽋㧔಴↥⋥ᓟ㧕 (b)ࠟ࡜ࠬൻ଻ሽߐࠇߚ૕⚦⢩ᩭ⒖ᬀ⢦߆ࠄᓧࠄࠇߚࠢࡠ࡯ࡦ↥੿㧔಴↥⋥ᓟ㧕 㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥ౏⴫ႎ๔╬

㧝㧕Ushijima H., Yoshioka H., Nagashima H., et al.㧦Improved survival of vitrified in vivo-derived porcine embryos. Journal of Reproduction and Development, 50, 481-486, 2004.

㧞㧕Esaki R., Ueda H., Nagashima H.,et al.㧦Cryopreservation of porcine embryos derived from in vitro matured oocytes. Biology of Reproduction, 71, 432-437, 2004.

㧟㧕Nagashima H., et al.㧦Production of Live Piglets Following Cryopreservation of Embryos Derived from In Vitro-Matured Oocytes. Biology of Reproduction, 76, 900-905, 2007.

b

a

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㘩ຠᱷᷲ߆ࠄࠛࠦࡈࠗ࡯࠼߳

㧔⇓↥࡮㘺ᢱ⺞ᩏᚲ ᓮᓇᐻ ਥቿ(ᣣᧄᄢቇ㕖Ᏹൕ⻠Ꮷ) 㒙ㇱ ੫㧕 ᐔᚑ 14㨪15 ᐕᐲᆔ⸤ޟ㘩ຠᱷᷲߩ㘺ᢱ೑↪ߦ㑐ߔࠆ✚ว⊛⎇ⓥޠ㧔ᣣᧄᄢቇ↢‛⾗Ḯ⑼ቇㇱ ᢎ᝼㧕 㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ ᐔᚑ 13 ᐕ 5 ᦬ߦ㘩ຠ࡝ࠨࠗࠢ࡞ᴺ(㘩ຠᓴⅣ⾗Ḯߩౣ↢೑↪╬ߩଦㅴߦ㑐ߔࠆᴺᓞ)߇ᣉⴕ ߐࠇޔ㘩ຠᓴⅣ⾗Ḯ(㘩ຠ⵾ㅧ೽↥‛ޔ㘩ຠᱷᷲ╬)ߩ㘺ᢱޔ⢈ᢱޔᴤ⢽࡮ᴤ⢽⵾ຠޔࡔ࠲ࡦ߳ ߩౣ↢೑↪ߩ㆏╭߇࿖ߦࠃߞߡ␜ߐࠇߚޕ ᐔᚑ 14 ᐕߦㄘᨋ᳓↥⋭ߩ㘺ᢱ໧㗴ᙣ⺣ળߢޔ㨬⥄⛎㘺ᢱߩჇ↥㨭ޔ㨬ᵹㅢ㘺ᢱߩวℂൻ㨭ޔ㨬㘺 ᢱߩ቟ోᕈ⏕଻㨭ޔ㨬⾗ḮᓴⅣဳ⇓↥ߩផㅴ㨭ޔ㨬࠮࡯ࡈ࠹ࠖࡀ࠶࠻ߣߒߡߩ஻⫾੐ᬺౣᬌ⸛ޠ ࠍᩇߣߔࠆޔޡ੹ᓟߩ㘺ᢱ᡽╷ޢ߇╷ቯߐࠇޔ㘩ຠᱷᷲ߆ࠄߩ㘺ᢱ↢↥߇᡽╷⺖㗴ߣߒߡขࠅ ਄ߍࠄࠇࠆߎߣߣߥߞߚޕ ߎߩࠃ߁ߥ⁁ᴫࠍ⢛᥊ߦో࿖ฦ࿾ߢ㘩ຠᱷᷲߩ㘺ᢱൻ੐ᬺ߇ዷ㐿ߒᆎ߼ࠆ߇ޔᒰᤨߪߘߩ ᄙߊ߇ੇ῎ಣℂߢ᳓ಽ฽㊂߇ 12%೨ᓟߩ☳ᧃ㘺ᢱࠍ⺞⵾ߔࠆᒻᘒߢ޽ࠅޔ㘺ᢱൻ੐ᬺ⠪ߪේ ᢱߩ㈩ว₸ࠍᩕ㙃ቇ⊛ߦ⸳⸘ߔࠆߎߣ߇ߥ߆ߞߚߚ߼ޔ⛎ਈ㊂ߥߤኅ⇓߳ߩ⛎ਈᴺࠍቯ߼ࠆ ߎߣ߇࿎㔍ߥ⁁ᴫߢ޽ߞߚޕ ߘߎߢޔᧄ⎇ⓥߢߪޔԘో࿖ฦ࿾ߢ↢↥ߐࠇߡ޿ࠆ㘩ຠᱷᷲੇ῎㘺ᢱߩ⚵ᚑߣᩕ㙃ଔࠍ⺞ ᩏߒޔ⚿ᨐࠍ⚫੺ߔࠆߎߣޔԙ㘩ຠᱷᷲੇ῎㘺ᢱߩᩕ㙃ଔ(TDN ฽㊂)ࠍ◲ᤃߦ᷹ቯߔࠆᚻᴺ ࠍ㐿⊒ߒޔ⵾ຠߩ TDN ฽㊂ࠍൻቇ⚵ᚑ߆ࠄ◲ᤃ࡮ㄦㅦߦ⹏ଔߢ߈ࠆࠃ߁ߦߔࠆߎߣޔԚේ ᢱߩടᾲੇ῎Ꮏ⒟ߢߩⰮ⊕⾰ߩᾲᄌᚑߣⰮ⊕⾰ߩᶖൻ₸ߣߩ㑐ㅪࠍ᣿ࠄ߆ߦߔࠆߎߣޔԛ㘩 ຠᱷᷲੇ῎㘺ᢱߩࠕࡒࡁ㉄⚵ᚑޔࡒࡀ࡜࡞฽㊂ߩ․ᓽࠍᛠីߒޔ⽋⢈⢒㘺ᢱߣߒߡ೑↪ߔࠆ ႐วߩ⇐ᗧὐࠍ᣿ࠄ߆ߦߔࠆߎߣ╬ࠍ⋡⊛ߣߒޔ⹜㛎ࠍታᣉߒߚޕ⹜㛎ߩਥⷐߥᚑ❣ߪએਅ ߩߣ߅ࠅߢ޽ࠆޕ 㧝㧕ടᾲߩߚ߼ߩੇ῎᷷ᐲ߇㜞ߊߥࠆߦ઻޿ޔ☻Ⱞ⊕⾰ߩ⽋ߢߩᶖൻ₸ߪૐਅߔࠆߎߣ߆ ࠄޔ㘩ຠᱷᷲੇ῎㘺ᢱߩ TDN ฽㊂ߩផቯᴺߢߪޔߘߩᓇ㗀ࠍട๧ߒߚᣇᴺ߇᮸┙ߐࠇߨ߫ ߥࠄߥ޿߇ޔ৻⥸ಽᨆߣ㉂⚛ಽᨆࠍ⚵ߺวࠊߖߚਅ⸥ߩផቯᑼࠍޔⰮ⊕⾰ߩᾲᄌᕈߩ⒟ᐲࠍ ෻ᤋߒߚ TDN ฽㊂ផቯᴺߣߒߡផᅑߔࠆߎߣ߇ߢ߈ߚޕ TDN=0.91×OCWนṁᕈ☻Ⱞ⊕⾰+2.21×☻⢽+1.1×(OCW+NCWFE)- 27.2

(OCW㧦✚❫⛽ޔNCWFE㧦♧࡮࠺ࡦࡊࡦ࡮᦭ᯏ㉄㘃ޔOCW นṁ☻Ⱞ⊕⾰㧦ో☻Ⱞ⊕⾰߆ࠄ OCW ਛߩ☻Ⱞ⊕

⾰฽㊂ࠍᏅߒᒁ޿ߚ୯) 㧞㧕105 ὐߩ㘩ຠᱷᷲੇ῎㘺ᢱߩᚑಽߣ TDN ฽㊂ߪ☻Ⱞ⊕⾰߇ 11.6~38.4%ޔ☻⢽⢌߇ 4.0~25.2%ޔTDN ߇ 23.1~106.1%ߩ▸࿐ߢᄌേߒޔ߫ࠄߟ߈߇㕖Ᏹߦᄢ߈޿ߎߣ߇ࠊ߆ߞߚޕ 㧟㧕㘩ຠᱷᷲੇ῎㘺ᢱߩᄙ↪ߢߪࠞ࡞ࠪ࠙ࡓޔ੝㋦ޔࡆ࠲ࡒࡦ Eޔ࡝ࠫࡦޔࡔ࠴ࠝ࠾ࡦ߇ ⷐ᳞㊂ߦḩߚߥ޿ߎߣ߇⠨߃ࠄࠇޔ⛎ਈߦ޽ߚࠅޔࠨࡊ࡝ࡔࡦ࠻ߩ⵬⛎߿㈩ว㘺ᢱߣߩઍᦧ ₸ߦ⇐ᗧߔࠆߴ߈ߢ޽ࠆߎߣ߇ᜰ៰ߢ߈ߚޕ 㧞㧚⎇ⓥ೔㆐ߩ⃻⁁ ᧄ⎇ⓥߩታᣉᓟޔᣣᧄᄢቇࠍᆎ߼ߣߒߡޔ㨬⢈⢒⽋ߩ㙃ಽⷐ᳞㊂ߦㆡวߐߖࠆߚ߼ߩේᢱߩ ㈩วޠޔ㨬Ꮢ⽼ߩ⽋⢈⢒↪㈩ว㘺ᢱߩ⚵ᚑߦㄭૃߐߖࠆߚ߼ߩේᢱߩ㈩ว㨭ޔ㨬Ꮢ⽼㈩ว㘺ᢱߩ 㘩ຠᱷᷲੇ῎㘺ᢱઍᦧ₸ߦ㑐ߔࠆ⎇ⓥ㨭߇ో࿖⊛ߦታᣉߐࠇߡ޿ࠆޕߘࠇࠄߩᚑᨐߩਛ߆ࠄޔ

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