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Diversity and Function of Aerobic Culturable Bacteria in the Intestine of Sea Cucumbers

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Diversity and Function of Aerobic Culturable Bacteria in the Intestine of Sea Cucumbers

December 2012

Graduate School of Science and Technology Nagasaki University

Xiaochi Zhang

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CONTENTS

CHAPTERⅠ Introduction ··· 1

CHAPTER Ⅱ Diversity and function of aerobic culturable bacteria in the intestine of the sea cucumber Holothuria leucospilota···7

2.1 Introduction··· 8

2.2 Materials and Methods··· 9

2.3 Results··· 13

2.4 Discussion ··· 18

2.5 Summary ··· 20

CHAPTER Ⅲ Physiological characterization of aerobic culturable bacteria in the intestine of the sea cucumber Apostichopus japonicus··· 33

3.1 Introduction··· 34

3.2 Materials and Methods··· 35

3.3 Results··· 40

3.4 Discussion ··· 45

3.5 Summary ··· 48

CHAPTER Ⅳ Diversity and function of aerobic culturable bacteria in the intestine of deep-sea holothurian ··· 59

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4.1 Introduction··· 60

4.2 Materials and Methods··· 61

4.3 Results··· 66

4.4 Discussion ··· 71

4.5 Summary ··· 74

CHAPTER Ⅴ General discussion ··· 85

Acknowledgments··· 98

References··· 100

参照

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