Protein Metabolism in the Haplocorm ofTimothy (Phleum pratense L.) after Cutting
その他（別言語等）
のタイトル
刈取後におけるチモシー球茎中の蛋白質代謝 著者（英） Mino Yosuke, Satake Yoshiyo
journal or
publication title
Research bulletin of Obihiro Zootechnical University. Series I
volume 10
number 4
page range 911‑916
year 1978‑06‑25
URL http://id.nii.ac.jp/1588/00002284/
R e s . B u l l . O b i h i r o U n i v . ， 10 ( 1 978) : 911‑‑916.
P r o t e i n M e t a b o l i s m i n t h e H a p l o c o r m o f T i m o t h y (Phleum ρ r a t e n s e L . ) a f t e r C u t t i n g
Y6suke MINO
特( L a b o r a l o r y 0 1 E n v i r o n m e n l a l B o l a n y ， O b i h
げoU n i v e r s i l y ) Yoshiyo SATAKE'
得 骨( H o k k a i d o C e n l r a l A g r i c u l t u r a l E x p e r i
剖e n l a lS t a t i o n ) R e c i e v e d November 1 5 ， 1 9 7 7
刈取後におけるチモシー球茎中の蛋白質代謝 美 濃 羊 輔
h佐 竹 芳 世 帥
Introduction
911
Many workers
l，
4，
8， 1 O ， 1 l ，
la，
16)have i n v e s t i g a t e d t h e v a r i a t i o n o f c a r b o h y d r a t e s i n r e s e r v e o r g a n s d ur in g regrowth o f g r a s s e s a f t e r c u t t i n g . On t h e o t h e r hand ，乱 1 I L THORPE and DAVIDSON
10)s u g g e s t e d t h a t n i t r o g e n o u s compounds ， i n c l u d i n g p r o t e i n s ， may be a s i m p o r t a n t a s s o l u b l e c a r b o h y d r a t e s f o r regrowth o f g r a s s e s ， b u t no e n z y m o l o g i c a l s t u d y h a s been made on t h e p r o t e i n metabolism i n 耳 目 回 目 y e t . The pr 田 e n twork was a t t e m p t e d t o c l a r i f y t h e r e l a t i o n s h i p between p r o t e i n c o n c e n t r a t i o n and p r o t
師 団a c t i v i t yi n t h e haplocorm o f t i m o t h y a f t e r c u t t i n g
Materials and Methods Plant m a t e r i a l
The s e e d s o f t i m o t h y (Phleum p r a t e n s e L . c v . Climax) ( 4 0 0 s e e d s / m ' ) were sown i n t h e E x p e r i m e n t a l Farm o f O b i h i r o U n i v e r s i t y i n e a r l y May i n 1 9 7 5 . Each o f 5 kg o f N ， P ， O ， and K ， O was f e r t i l i z e d t o t h e e x p e r i m e n t a l p l o t ( 1 0 a ) i n e a r l y May i n 1975 and 1976 The s t e m s o f t i m o t h y were c u t a t a b o u t 10 cm above s o i l s u r f a c e on 2 0 t h o f J u l y i n 1 9 7 6 . N o n ‑ d i s e a s e d haplocorms were c o l l e c t e d and washed with t a p w a t e r .
Prepa
γ' a t i o n o[ crude enzyme s o l u t i o n
Fourty grams o f haplocorms were c u t i n t o s l i c e s ( a b o u t 1 m m i n t h i c k n e s s ) with a s c a l p e l . The s l i c e s were homogenized i n 160 ml o f p h o s p h a t e bu 旺 e r(M/90 ， pH 6 . 8 ) f o r 5 min u s i n g a m i x e r . The homogenate was s q u e e z e d through gauze ， f o l l o w e d by c e n t r i f u g a . t i o n a t 10 ， 000 g a t 5
0C f o r 10 m i n . The s u p e r n a t a n t ， 五 l t e r e dthrough a 品 l t e rp a p e r ， was
普
