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649-6493 930
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The “sneak” preview of the Marchantia polymorpha genome Key words: bryophyte, genetic redundancy, liverwort
Katsuyuki T. Yamato1, Takayuki Kohchi2
1Department of Biotechnological Science Kinokawa 649-6493, Japan
2Graduate School of Biostudies, Kyoto University Kyoto 606-8502, Japan
Marchantia polymorpha L.
(The Arabidopsis Genome Initiative 2000) (International Rice Genome Sequencing Project 2005)
(Rensing et al. 2008) 30
http://www.phytozome.net/ http://www.plantgdb.org/
1986 (Ohyama et al. 1986) (Shinozaki et al. 1986) 120 kb 160 kb
K. T. Yamato & T. Kohchi-2 50 kb (Sanger et al. 1982)
1992
(Oda et al. 1992)
3 2
2007 Y 10 Mb
(Yamato et al. 2007 大和勝幸 2009)
Y (Skaletsky et al. 2003)
(Kuroki et al. 2006) 3
2008 X
Joint Genome Institute (JGI) Monash John Bowman
(http://www.jgi.doe.gov/sequencing/why/99191.html)
(河 内孝之 石崎公庸 2012)
JGI
P1 PAC
(Okada et al. 2000) 30
20 PAC
JGI 10
PAC
3.3 Mb 280 Mb (Okada et al.
2000) 1% DNA GC 40%
PAC
1 100 kb 1
1
EST 1
10 kb 0.7
10 kb 2.3 TAIR10
pMM23-619A2
EST 18,000 20,000
0.7/10 kb = 20,000 /280 Mb
K. T. Yamato & T. Kohchi-4 pMM23-619A2
NCBI NR NCBI non-redundant protein sequences Atha, Smol Ppat
Mpol EST EST
4 6 tRNA tRNA
miRNA RNA
(Floyd and Bowman 2004) rRNA
9 X 1
(Fujisawa et al. 2003, Sone et al. 1999)
JGI
Takaragaike-1 Kitashirakawa-2 (河内孝之 石崎公庸 2012)
30 PAC
31 EST
dCAPS (derived cleaved amplified polymorphic sequences) SSR (simple sequence repeats)
78 109
8 900 cM
JGI Takaragaike-1 Kitashirakawa-2
F1
Kitashirakawa-2 JGI
Takaragaike-1 Kitashirakawa-2
K. T. Yamato & T. Kohchi-6
JGI 2
Fosmid Roche GS-FLX
27x 2011 12
ver. 0.6 ver. 0.6
8,400 200 Mb 70
109
X 9
JGI EST 90%
cDNA
5’/3’ (UTR)
mRNA 5’ AUG
ATG ATG
UTR-ATG
5’UTR
2
10 ATG
EST
5
(Strasser et al. 2010)
(Leivar and Quail 2011)
(Schaefer
2001) (Rensing et al. 2008)
(Rensing et al. 2007)
LFY
2 (荒木崇 2012)
PHYTOCHROME INTERACTIG FACTOR
(PIF) 1
K. T. Yamato & T. Kohchi-8
CLE 32
(澤進一郎2012) (Sasaki et al. 2007)
(河内孝之 石崎公庸 2012)
8 X n =
8 + X Y n = 8 + Y Y X
Y Y
(Yamato et al. 2007) Y 0.1 / 10 kb
0.7 X
X Y
X X
JGI ver. 0.6 3.9 Mb Y 64
20 X
X Y
X Y
Y (Egydio de Carvalho et al. 2002) (Lorenzetti et al. 2004)
(Ikeda et al. 2007)
(Hirai et al. 2008, Mori et al. 2006)
X Y
JGI
Kitashirakawa-2 JGI
K. T. Yamato & T. Kohchi-10
JGI Monash John Bowman Sandra
Floyd
cDNA
X Y
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K. T. Yamato & T. Kohchi-12
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