Expression of GUS‑gene in monocot temperate grasses by an improved particle gun
その他（別言語等）
のタイトル
パーティクルガンによる単子葉イネ科牧草における GUS遺伝子の発現
著者（英） Horikawa Yoh, Ohi Hiroyuki, Kakuta Hideo journal or
publication title
Research bulletin of Obihiro University.
Natural science
volume 18
number 3
page range 143‑146
year 1993‑10‑25
URL http://id.nii.ac.jp/1588/00001956/
R e s . B u l l . O b i h i r o U n i v . ， 1 8 ρ 9 9 3 ) : 1 4 3 ‑ 1 4 6
Expression o f GUS‑gene i n monocot temperate grasses by an improved p a r t i c l e gun
Yoh HORIKAWA' ， Hiroyuki OH I ' ， and Hideo KAKUTA' (R
即 時v e d: May 3 1 ， 1 9 9 3 )
Summary
S u c c e s s f u l d e l i v e r y o f f o r e i g n g e n e s t o i n t a c t c a l l u s e s o f monocot orchardgrass (D α c t y l i s glomen α
臼L . ) and timothy (Phleum p r a t e n s e L.) was demonstrated by u s i n g an improved p a r t i c l e g u n . T h i s b i o l i s t i c d e v i c e was equipped w i t h a p o l y a c ‑ e t a l m a c r o p r o j e c t i l e w i t h GUS ‑gene c o a t e d g o l d p a r t i c l e s ， d r i v e n by gas p r e s s u r e from a helium c y l i n d e r c o n t r o l l e d by a s o l e n o i d v a l v e . The p a r t i c l e g u n i s a w i d e d l y u s e f u l t r a n s f o r m a t i o n method c a p a b l e o f overcoming t h e h o s t ‑range r e s t r i c t i o n s o f Agrob α c t e r i u m and t h e r e g e n e r a t i o n problems o f p r o t p l a s t t r a n s f o r m a t i o n .
Key words P a r t i c l e gun ， T r a n s i e n t g e n e e x p r e s s i o n ， GUS (βGulcuronidase) ， D a c t y l i s g l o m e r a t αL.， Phleum p r a t e n s e L . ，
1 4 3
Introduction
Agrob α ctenum ， d i r e c t DNA t r a n s f e r (PEG / calcium p h o s p h a t e o r e l e c t r o p o l a t i o n ) ， and m i c r o i n j e c t i o n a r e u s e f u l f o r t h e i n t r o d u c t i o n o f f o r e i g n DNA i n t o p l a n t c e l l s i n some p l a n t s However ， t h e y a r e n o t g e n e r a l l y u s e f u l b e c a u s e o f v a r i o u s b a r r i e r R T h e s e b a r r i e r s i n c l u d e l i m i t ‑ e d h o s t range ， low e f f i c i e n c y and d i f f i c u l t y i n r e g e n e r a t i n g p l a n t s from t h e t r a n s f o r m e d t i s s u e s . The u s e o f m i c r o p a r t i c l e s a s DNA c a r r i e r s might overcome t h e s e o b s t a c l e s .
gas p r e s s u r e have been used f o r p a r t i c l e a c c e l e r ‑ a t i o n . I n s p i t e o f t h e numerous a d v a n t a g e s o f t h e s i m p l e p a r t i c l e gun d r i v e n by compressed a i r o r n i t r o g e n gas ， i t i s n o t n e c e s s a r i l y easy t o bom‑
bard p l a n t samples i n a l a r g e v a r i e t y o f d i f f e r e n t forms (Morikawa e t a l . ， 1 9 8 9 ) ，
S i n c e t h e fundamental s t u d i e s by K l e i n e t a l . ( 1 9 8 7 ) and C h r i s l o u e t a l . ( 1 9 8 8 ) ， . t h e m i c r o ‑ p r o j e c t i l e ‑ m e d i a t e d DNA t r a n s f e r t e c h n i q u e has become a w i d e l y u s e f u l m e t h o d . I n p r e v i o u s r e p o r t s ，
gunpo、~der，a r c d i s c h a r g e e x p l o s i o n and
Kakuta e t a l . ( 1 9 9 2 ) improved a s i m p l e p a r t i c l e gun ， i n w h i c h t h e m a c r o p r o j e c t i l e was a c c e l e r a t e d from t h e u p p e r p a r t w i t h p r e s s u r e ‑ c o n t r o l l e d g a s f o r e a s y bombardment o f d i f f e r e n t k i n d s o f sam‑
p l e s ， Using t h i s d e v i c e ， we o b s e r v e d t h e i n t r o ‑ d u c t i o n . o f t h e r e p o r t e r gene f o r GUS (βglu‑
c u r o n i d a s e ) i n monocot t e m p e r a t e g r a s s e s ' c a l l u s e s
，1
Laboratory o f Forage Crop S c i " O b i h i r o U n i v e r s i t y o f Agr. & V e t . Medicine ， O b i h i r o ， Hokkaido 0 8 0
2
