〈平成10〜20年度 研究開発委託事業成果からの展望〉
畜産技術の開発活用促進事業
研究開発の成果と展開方法
平成20年12月
⋡ ᰴ
㧝ࡉ࠲ਥⷐ⚵❱ㆡวⶄว/*%㗔ၞߩㆮવሶ᭴ㅧ⸃ᨆߩㅴዷߣዷᦸ㧔⮮㤗ሶ㧕̖̖
㧞ᩭ⒖ᬀ↪ෆᲣ⚦⢩ߩಓ⚿ሽᛛⴚߩㅴዷ㧔⧎↰┨㧕̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖
㧟⇓↥♽ឃ᳓ߩࡔ࠲ࡦ⊒㉂ಣℂᛛⴚߣ⊒㉂ᶧߩ⢈ᢱォ↪ᛛⴚߩዷᦸ㧔ේ↰⑲᮸㧕̖̖̖
㧠࠙ࠪෆᲣ⚦⢩ߩᄖᚑᾫၭ㙃ᦼ㑆ߦ₪ᓧߔࠆ⢦⊒↢⢻ߦ߷ߔࡒ࠶࠼ࠞࠗࡦߩ
ലᨐߦ㑐ߔࠆ⎇ⓥߩㅴዷߣታ↪ൻ߳ߩዷᦸ㧔ጊ↰㓷㧕̖̖̖̖̖̖̖̖̖̖̖̖̖
㧡㘩ᢱ⥄⛎₸ะߩߚߩᨋ㑆’ࠪࠬ࠹ࡓߩౣ᭴▽㧔⮮ⴐ㧕̖̖̖̖̖̖̖̖̖
㧢ሶ࠙ࠪߩઍ⻢ᓮᛛⴚߩㅴዷߣຠ㐿⊒㧔ዊේཅᤘ㧕̖̖̖̖̖̖̖̖̖̖̖̖̖̖
㧣ኅ⇓ឃᴭ‛ߩಣℂၸ⢈ൻߦ㑐ࠊࠆᓸ↢‛ߣߘߩᵴ↪㧔ਛ㧕̖̖̖̖̖̖̖̖̖
㧤⚦⢩ࠢࡠࡦኅ⇓ߩߦ߁ㆮવ⊛ోᕈߩ⹏ଔ㧔࿖ᨑືᄦ㧕̖̖̖̖̖̖̖̖
㧥ࡉ࠲⢦ಓ⚿ሽߩᛛⴚ㕟ᣂ㧔㐳᎑Ყํᔒ㧕̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖
㘩ຠᱷᷲ߆ࠄࠛࠦࡈࠖ࠼߳㧔㒙ㇱ੫㧕̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖
⚦⢩ࠢࡠࡦኅ⇓ߦ↱᧪ߔࠆ↢↥‛ߩห╬ᕈ⸃ߩߚߩ⺖㗴㧔ᷰㄝિ㧕̖̖̖
⇓↥㗔ၞߦ߅ߌࠆๆ᷹ቯߦࠃࠆ⢦ຠ⾰⹏ଔߩዷᦸ㧔㒙ㇱብਯ㧕̖̖̖̖̖̖̖̖̖
᛫↢‛⾰ߦઍࠊࠆ⽋↪᛫⩶ߩ㐿⊒㧔ደ૫♿㧕̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖̖
⚻∛ේᕈ⣖Ἳဳࠕࠞࡃࡀ࠙ࠗ࡞ࠬߩ∛ේᕈ⸃ᨆߣ⸻ᢿᴺߩ⏕┙㧔⍹ඳ⤿㧕̖̖
㜞ᴲ‐ߦ߅ߌࠆฃ⢝₸ૐਅߩⷐ࿃ߣሶችୃᓳ⹏ଔᴺߩ㐿⊒㧔ዬኅ⟵ᤘ㧕̖̖̖̖
ߪ ߓ ߦ
․␠࿅ᴺੱ⇓↥ᛛⴚදળߪޔ⁛┙ⴕᴺੱㄘ⇓↥ᬺᝄ⥝ᯏ᭴ߩ⇓↥ᝄ⥝ᬺߦࠃࠆ
ഥࠍฃߌߡޔᐔᚑ10ᐕᐲ߆ࠄޟ⇓↥ᣂᛛⴚ㐿⊒ᵴ↪ଦㅴᬺޠ㧔ᐔᚑ19ᐕᐲ߆ࠄኅ⇓↢↥
ᣂᛛⴚലᵴ↪✚วኻ╷ᬺߩਛߩޟ⇓↥ᣂᛛⴚߩ㐿⊒ᵴ↪ଦㅴᬺޠ㧕ࠍታᣉߒߡ߈߹ߒ ߚޕ
ᧄᬺߪޔߐ߹ߑ߹ߥ⇓↥ᛛⴚߩ㐿⊒߳ߩⷐᦸߦኻᔕߔࠆߚޔ⇓↥ో⥸ߦࠊߚࠆ⎇ⓥ
ߩផㅴߣߎࠇࠍၮ⋚ߣߒߚᣂᛛⴚߩ㐿⊒߿ᵴ↪ࠍଦㅴߔࠆߎߣࠍ⋡⊛ߣߒޔ10 ᐕࠅߩ
ᬺታᣉᦼ㑆ౝߦᆔ⸤ߒߚ⎇ⓥ㐿⊒⺖㗴ߪ⇓↥ోಽ㊁ߦࠊߚࠅޔᑧߴ94⺖㗴ߦߩ߷ࠅ߹ߔޕ ߎߩౠሶߢ⚫ߔࠆᣂߒ⇓↥ᛛⴚߪޔߎߩᬺߢᆔ⸤ޔታᣉߒߚ⺖㗴ߩਛ߆ࠄޔ
․ߦ㑐ᔃ߇ᜬߚࠇߡࠆߣᕁࠊࠇࠆචᢙ⺖㗴ࠍㆬቯߒޔ⎇ⓥࠍᜂᒰߐࠇߚᣇߦ߅㗿ߒߡ ߘࠇߙࠇߩ⺖㗴ߦ߅ߌࠆ⎇ⓥᚑᨐࠍၮߦޔߘߩಽ㊁ߩ⎇ⓥ㐿⊒ߩ⁁ޔᓟߩᣇะ⺖㗴
╬ߦߟߡߏၫ╩ߚߛߚ߽ߩߢߔޕ
ㅜޔ⎇ⓥࠍᆔ⸤ߒߚ⊝᭽߆ࠄὐᬌޔ⏕ߚߛߚోᆔ⸤⺖㗴ߩᚑᨐࠍขࠅ߹ߣߚ
ᬺోߩႎ๔ᦠߣߣ߽ߦޔ࿖߮ㇺᐭ⋵ߩ⇓↥㑐ଥ⠪ߦ㈩Ꮣߔࠆ߶߆ޔ⇓↥ᛛⴚදળ ߩᯏ㑐߿ࡎࡓࡍࠫ╬ߢ߽⚫ߚߒ߹ߔޕ⎇ⓥ߿ᛛⴚ㐿⊒ޔߘߩᡰេ╬ߦ៤ࠊࠆᣇޘ ߩෳ⠨ߦߥࠇ߫ᐘߢߔޕ
ᐔᚑ20ᐕ12
․␠࿅ᴺੱ⇓↥ᛛⴚදળ
ࡉ࠲ਥⷐ⚵❱ㆡวⶄว/*%㗔ၞߩㆮવሶ᭴ㅧ⸃ᨆߩㅴዷߣዷᦸ
㧔᧲ᶏᄢቇකቇㇱ ⻠Ꮷ ⮮㤗ሶ㧕
ᐔᚑ9㨪10ᐕᐲᆔ⸤ޟኅ⇓ߦ߅ߌࠆਥⷐ⚵❱ㆡวⶄว(MHC)㗔ၞߩㆮવሶ᭴ㅧ⸃ᨆޠ 㧔␠࿅ᴺੱㄘᨋ᳓↥వ┵ᛛⴚ↥ᬺᝄ⥝ࡦ࠲ བྷ⸤㧕
㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ
ࡉ࠲ߩਥⷐ⚵❱ㆡว᛫ේⶄว(MHC)ߢࠆSwine Leukocyte Antigen(SLA)ߪޔߘߩ᛫
ේဳ߇ࡢࠢ࠴ࡦធ⒳ߦࠃࠆ᛫↥↢⢻ߥߤߩ∉ᔕ╵ᕈ߿ࡔࡁࡑ⊒∝ߣߩ⋧㑐߇⍮ࠄࠇޔ
⇓↥ቇಽ㊁ߦ߅ߌࠆఝࠇߚ᛫∛ᕈ߿⚻ᷣᒻ⾰ࠍᜬߟࡉ࠲ߩㆬᛯߦ㑐ਈߒߡࠆߣ⠨߃ࠄࠇߡ
ࠆޕ߹ߚޔࡉ࠲ߪ⸃೬ޔ↢ℂቇ⊛ߦࡅ࠻ߣ㘃ૃᕈ߇㜞ߊޔⴊᶧ↢ൻቇ⊛ᢙ୯߽ࡅ࠻ߣㄭ
ߎߣ߆ࠄޔࡅ࠻߳ߩ⇣⒳⒖ᬀ࠼࠽ߣߒߡᵈ⋡ߐࠇߡࠆઁޔ⢈ḩ߿♧ዩ∛ߥߤߩ∔ᖚࡕ࠺
࡞േ‛ޔක≮ᛛⴚࠪࡒࡘ࡚ࠪࡦߥߤࠍ⋡⊛ߣߒߚታ㛎േ‛ߣߒߡᄙ↪ߐࠇߡࠆޕߎࠇ ࠄߩ⇓↥ቇಽ㊁ߦ߅ߌࠆఝࠇߚ᛫∛ᕈ߿⚻ᷣᒻ⾰ࠍᜬߟࡉ࠲ߩㆬᛯޔਗ߮ߦකቇޔታ㛎േ‛
ቇಽ㊁ߦ߅ߌࠆห⒳⇣⒳⒖ᬀ⎇ⓥߩផㅴ߿SLA࿕ቯ⚐♽ࡒ࠾ࡉ࠲ߩߦ㊀ⷐߥၮ⋚⊛ᖱ ႎߩឭଏޔ߅ࠃ߮᭽ޘߥേ‛⒳㑆ߩMHCࠥࡁࡓᖱႎᲧセࠍ⋡⊛ߣߒߡޔSLA㗔ၞߩࠥࡁࡓ
⸃ᨆߣᄙဳᕈ⸃ᨆࠍⴕߞߚޕ 㧞㧚⎇ⓥ㆐ߩ⁁
ࠫࡎࡢࠗ࠻⒳⽋(Hp-1a.1 ࡂࡊࡠ࠲ࠗࡊ)ߩ SLA ో㗔ၞ(2.4Mb)ߩႮၮ㈩⸃⺒ߪޔ 2006 ᐕߦ╩⠪ࠄߩࠣ࡞
ࡊࠍว⸘㧟ࠣ࡞ࡊ ߩ࿖㓙⊛ߥห⎇ⓥߦࠃ ࠅቢੌߒޔߘߩㆮવሶ᭴ᚑ ߩోኈ߇ࠄ߆ߦߥߞߚ
1)ޕߎߩ㈩ᖱႎ߆ࠄޔ SLA 㗔ၞߦߪޔ27 ߩ SLA ㆮ વ ሶ ࠍ ว ⸘ 151 ߩㆮવሶ߇หቯߐ ࠇ(࿑ 1)ޔߎߩ㗔ၞߩࡑࠗ
ࠢࡠࠨ࠹ࠗ࠻ߩಽᏓߣ ᄙဳᕈ 2)ߥߤߩ․ᓽ߽
ࠄ߆ߦߥࠅޔએਅߦㅀߴࠆ ᛛⴚታ↪ൻ߳ߩၮ⋚ࠍ᭴
▽ߔࠆߎߣ߇ߢ߈ߚޕ
࿑1 SLA㗔ၞߩㆮવሶ᭴ᚑ 㧟㧚ᛛⴚታ↪ൻߩ⁁
೨㗄ߢㅀߴߚSLA㗔ၞߩႮၮ㈩ᖱႎߦၮߠ߈ޔ⒳ޘߩຠ⒳ߩSLAㆮવሶߩᄙဳ⸃ᨆࠍ ⴕޔߎࠇ߹ߢߦࠢࠬI ߅ࠃ߮ࠢࠬII ㆮવሶߩ6ㆮવሶᐳߦߟߡޔว⸘20⒳㘃ߩᣂ ࠕ࡞ࠍߒߚޕߐࠄߦޔ࿖ౝᄖߢSLAㆮવሶߩᄙဳ⸃ᨆ߇ㅴዷߒޔ200⒳ࠍ߃ࠆ
䋺 ⊒ 㪪㪣㪘㩷䉪䊤䉴 㪠㩷㪃䉪䊤䉴 㪠㩷᭽ㆮવሶ䋺 㪎 䋺 㪪㪣㪘㩷䉪䊤䉴 㪠㩷㪃㩷䉪䊤䉴 㪠㩷᭽னㆮવሶ䋺 㪋 䋺 ⊒ 㪪㪣㪘㩷䉪䊤䉴 㪠㪠㪃㩷䉪䊤䉴 㪠㪠㩷᭽ㆮવሶ䋺 㪏㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷 䋺 㪪㪣㪘㩷䉪䊤䉴 㪠㪠㪃㩷䉪䊤䉴 㪠㪠㩷᭽னㆮવሶ䋺 㪏
䋺 ⊒䇮䉁䈢䈲⊒น⢻ㆮવሶ䋺 㪈㪈㪉
䋺 ᣂ⊒น⢻ㆮવሶ䋺 㪊
䋺 னㆮવሶ䋺 㪐
㪪㪣㪘ㆮવሶ
㕖㪪㪣㪘ㆮવሶ
⸘ 㪈㪌㪈
MCCD1BAT1ATP6V1G2NFKBIL1LTA TNFLTB BX548169.9LST1NCR3AIF1BAT2BAT3APOMC7H6orf47BAT4CNK2BLY6G5B LY6G5C BAT5LY6G6E LY6G6D LY6G6C C7H6orf25DDAH2CLIC1MSH5C7H6orf26C7H6orf27VARS2LSM2HSPA1LHSPA1AHSPA1BAL773527.9NEU1C7H6orf2BAT8ZBTB12C2BFRDBPSKIV2LDOM3ZSTK19C4ACYP21A2 TNXBCREBL1FKBPLC7H6orf31PPT2EGFL8AGPAT1RNF5AGERPBX2GPSM3NOTCH4BTNL5BTNL6
ࡦ࠻ࡠࡔࠕ
BTNL4BTNL3BTNL2DRA DRB4 DRB3 DRB2 DRB1 DQA DQB2DQB1DOB2BX323833.8BX323833.9DRB5 DYB DOB1TAP2PSMB8TAP1PSMB9DMBDMABRD2 DOA COL11A2RXRBSLC39A7HSD17B8RING1 UBD ORF43-3 ORF43-2 ORF-1GABBR1MOGZfp57C7H6orf12 ZNRD1PPP1R11RNF39TRIM31TRIM40TRM10TRM15TRIM26AFP RAP1ASLA-1 SLA-5 SLA-9 SLA-3 SLA-2 SLA-4 SLA-11TRIM39RPP21GNL1PRR3 ABCF1PPP1R10MRPS18BC7H6orf134C7H6orf136DHX16AB1133356.6 NRMMDC1TUBB FLOT1IER3AB11335.2DDR1 GTF2H4VARS2L AB113354.6DPCR1C7H6orf205C7H6orf15 AB1113354.10PSORS1C1PSORS1C2CCHCR1TCF19POU5F1MIC2MIC1SLA-8 SLA-7 SLA-6 ࠹ࡠࡔࠕ
ࠢࠬ+Dㆮવሶ⟲
ࠢࠬ+㗔ၞ
ࠢࠬ+Cㆮવሶ⟲
ࠢࠬ+++㗔ၞ
ࠢࠬ++㗔ၞ
િ㐳ࠢࠬ++㗔ၞ
િ㐳ࠢࠬ+㗔ၞ
࠹ࡠࡔࠕ
䋺 ⊒ 㪪㪣㪘㩷䉪䊤䉴 㪠㩷㪃䉪䊤䉴 㪠㩷᭽ㆮવሶ䋺 㪎 䋺 㪪㪣㪘㩷䉪䊤䉴 㪠㩷㪃㩷䉪䊤䉴 㪠㩷᭽னㆮવሶ䋺 㪋 䋺 ⊒ 㪪㪣㪘㩷䉪䊤䉴 㪠㪠㪃㩷䉪䊤䉴 㪠㪠㩷᭽ㆮવሶ䋺 㪏㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷㩷 䋺 㪪㪣㪘㩷䉪䊤䉴 㪠㪠㪃㩷䉪䊤䉴 㪠㪠㩷᭽னㆮવሶ䋺 㪏
䋺 ⊒䇮䉁䈢䈲⊒น⢻ㆮવሶ䋺 㪈㪈㪉
䋺 ᣂ⊒น⢻ㆮવሶ䋺 㪊
䋺 னㆮવሶ䋺 㪐
㪪㪣㪘ㆮવሶ
㕖㪪㪣㪘ㆮવሶ
⸘ 㪈㪌㪈
MCCD1BAT1ATP6V1G2NFKBIL1LTA TNFLTB BX548169.9LST1NCR3AIF1BAT2BAT3APOMC7H6orf47BAT4CNK2BLY6G5B LY6G5C BAT5LY6G6E LY6G6D LY6G6C C7H6orf25DDAH2CLIC1MSH5C7H6orf26C7H6orf27VARS2LSM2HSPA1LHSPA1AHSPA1BAL773527.9NEU1C7H6orf2BAT8ZBTB12C2BFRDBPSKIV2LDOM3ZSTK19C4ACYP21A2 TNXBCREBL1FKBPLC7H6orf31PPT2EGFL8AGPAT1RNF5AGERPBX2GPSM3NOTCH4BTNL5BTNL6
ࡦ࠻ࡠࡔࠕ
BTNL4BTNL3BTNL2DRA DRB4 DRB3 DRB2 DRB1 DQA DQB2DQB1DOB2BX323833.8BX323833.9DRB5 DYB DOB1TAP2PSMB8TAP1PSMB9DMBDMABRD2 DOA COL11A2RXRBSLC39A7HSD17B8RING1 UBD ORF43-3 ORF43-2 ORF-1GABBR1MOGZfp57C7H6orf12 ZNRD1PPP1R11RNF39TRIM31TRIM40TRM10TRM15TRIM26AFP RAP1ASLA-1 SLA-5 SLA-9 SLA-3 SLA-2 SLA-4 SLA-11TRIM39RPP21GNL1PRR3 ABCF1PPP1R10MRPS18BC7H6orf134C7H6orf136DHX16AB1133356.6 NRMMDC1TUBB FLOT1IER3AB11335.2DDR1 GTF2H4VARS2L AB113354.6DPCR1C7H6orf205C7H6orf15 AB1113354.10PSORS1C1PSORS1C2CCHCR1TCF19POU5F1MIC2MIC1SLA-8 SLA-7 SLA-6 ࠹ࡠࡔࠕ
ࠢࠬ+Dㆮવሶ⟲
ࠢࠬ+㗔ၞ
ࠢࠬ+Cㆮવሶ⟲
ࠢࠬ+++㗔ၞ
ࠢࠬ++㗔ၞ
િ㐳ࠢࠬ++㗔ၞ
િ㐳ࠢࠬ+㗔ၞ
࠹ࡠࡔࠕ
ࠕ࡞߿ࡂࡊࡠ࠲ࠗࡊߩฬ⒓߇╩
⠪ࠍ 6 ฬߩᆔຬ߆ࠄ᭴ᚑߐࠇ ࠆ ISAG SLA Nomenclature Committee ߦࠃࠅቯߐࠇޔߎࠇ ࠄߩᖱႎߪޔ᭽ޘߥಽ㊁ߩMHC⎇
ⓥߦ⽸₂ߒߡࠆ(http://www.ebi.
ac.uk /ipd/mhc/sla/index.html㧕ޕ
߹ߚޔSLA 㗔ၞߩࠥࡁࡓ߿ SLA cDNA ࠢࡠࡦߩႮၮ㈩ᖱႎߦ ၮ ߠ ߡ 㐿 ⊒ ߒ ߚ ◲ ଢ ߥ SLA-DNA ࠲ࠗࡇࡦࠣᴺࠍ↪ߡޔ ߎ ࠇ ߹ ߢ ᶏ ᄖ ߢ ႎ ๔ ߐ ࠇ ߡ ࠆ
NIHࡒ࠾ࡉ࠲ߥߤߩSLA࿕ቯࡉ࠲ߣߪ⇣ߥࠆᣂࡂࡊࡠ࠲ࠗࡊࠍᜬߟ2ຠ⒳(ࠢ࠙ࡦࡒ࠾ࡉ
࠲ޔ࠺ࡘࡠ࠶ࠢ⒳ࡉ࠲3))ߩࡉ࠲ࠍߒߚ(1)ޕߎࠇࠄߩSLA࿕ቯࡉ࠲ߩਛߢޔࠢ࠙ࡦ ࡒ࠾ࡉ࠲ߦߟߡߪޔ(ᩣ)ࠫࡖࡄࡦࡈࠔࡓࠃࠅޔSLA ࿕ቯࡉ࠲ߣߒߡ⽼ᄁଏ⛎ߐࠇޔකᎿ ቇ↪ࠍೋߣߔࠆ᭽ޘߥಽ㊁ߢታ㛎േ‛ߣߒߡᵴ↪ߐࠇߡࠆޕ
㧠㧚ᓟߩᣇะޔ⺖㗴
ߎࠇ߹ߢߦ⸃ᨆߒߚSLA㗔ၞߩࠥࡁࡓ㈩ߣࡑࠗࠢࡠࠨ࠹ࠗ࠻ࡑࠞ߿SLAㆮવሶ ߩᄙဳᕈᖱႎߪޔ2009ᐕߦ⸃⺒ቢੌ੍ቯߩࡉ࠲ోࠥࡁࡓ㈩ᖱႎߣวࠊߖߡޔ∔ᖚᗵฃᕈ߿
ᛶ᛫ᕈߦ㑐ਈߔࠆ∉㑐ㅪㆮવሶࠍ᭽ޘߥㆮવሶߩ✂⟜⊛ߥᄙဳ⊒⸃ᨆ߳⊒ዷߒޔ ฦ⒳∔ᖚᗵฃᕈߦ㑐ࠊࠆᯏ᭴⸃߳ߣ⎇ⓥ߇ㅴዷߔࠆߣ⠨߃ࠄࠇࠆޕ
৻ᣇޔ╩⠪ࠄ߇㐿⊒ߒߚ 2 ຠ⒳ߩ SLA ࿕ቯࡉ࠲ߪޔห⒳߅ࠃ߮⇣⒳⤳ེ⒖ᬀታ㛎ߦࠃࠆ MHC ㆡวᐲߣ੍ᓟߣߩ㑐ㅪᕈ⸃ᨆߩઁޔ∉㑐ㅪ⮎ߩ㐿⊒ޔᛩ⮎ࠪࡒࡘ࡚ࠪࡦ߿∔
ᖚࡕ࠺࡞߅ࠃ߮ౣ↢ක≮ߥߤߩක≮ಽ㊁ߦ߅ߡޔታ㛎േ‛ߣߒߡߩ࿖ౝᄖߩ⎇ⓥ↪ߩჇ ᄢ߇ᦼᓙߐࠇࠆޕ߹ߚޔ᛫∛ᕈຠ⒳ߩㆬᛯࡑࠞߣߒߡߩSLAㆮવሶߩ↪ᕈߩ⸃ߩߚ
ߦޔSLA࿕ቯࡉ࠲ࠍ↪ߚޔࡢࠢ࠴ࡦធ⒳ߥߤߩฦ⒳ᄖ᧪᛫ේߦኻߔࠆ∉ᔕ╵ᕈߣSLA
࠲ࠗࡊߣߩ⋧㑐⸃ᨆ߇ㅴⴕਛߢࠆޕߎߩࠃ߁ߥ⎇ⓥߦട߃ߡޔวᚑࡍࡊ࠴࠼ߥߤߩ⒳ޘߩ
∔ᖚ㑐ㅪ᛫ේߣߩ㑐ㅪᕈࠍ⸃ᨆߔࠆߎߣߦࠃࠅޔᗵᨴ∝ߦኻߔࠆ∔ᖚᗵฃᕈ߿ᛶ᛫ᕈ߅ࠃ߮
ࡢࠢ࠴ࡦ㐿⊒ޔߐࠄߦ⚻ᷣᒻ⾰ߣߩ㑐ㅪᕈߦߟߡ߽↪ߥᖱႎ߇ᓧࠄࠇࠆޕએߩࠃ߁ߦޔ SLA࿕ቯࡉ࠲ߪޔ᭽ޘߥಽ㊁ߩࡃࠗࠝ࠰ࠬߣߒߡᭂߡ㊀ⷐߢࠆߣ⠨߃ࠄࠇޔᣂߚߥ
⇓↥㑐ㅪ↥ᬺߩ㐿ᜏߦ❬ߍࠆߚߦ߽ޔSLA ࿕ቯࡉ࠲ߩ♽⛔⛽ᜬߣታ㛎േ‛ߣߒߡߩଏ⛎ޔ ߅ࠃ߮᭽ޘߥᛛⴚ㐿⊒⾗㊄ߩᡰេ߇ಾߦᦼᓙߐࠇࠆޕ
㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥႎ๔╬
㧝㧕Renard C., et al.㧦Genomics 88, 96-110, 2006.
㧞㧕Ando A., et al.㧦 Immunogenetics 60, 399-407, 2008.
㧟㧕Soe OK., et al.㧦 Xenotransplantation, In press.
ຠ⒳ (ᣥฬ⒓) SLA-1 SLA-2 SLA-3 DRB1 DQB1
Clawn 16.16 (c1) *0401 *w09an02 *0602 *11ac21 *0601
17.17 (c2) null? *06an03 *03an02 *0801 *0501
16.17(c3) *0401 *w09an02 *0602 *0801 *0501 17.16(c4) null? *06an03 *03an02 *11ac21 *0601
Duroc 27.30 (d1) *08an03, *06an04 *0102 *0101 *1101 *0503
60.13 (d2) *-an02 *1002 *0502 *0403 *0303
Large White 1.1 (H01) *0101 *0101 *0101 *0101 *0101
NIH (MGH) 2.2 (a) *0201, *0701 *0201 null *0201 *0201
3.3 (c) null *0301 *0301 *0301 *0301
4a.4 (d) *0401 *0401 *0401 *0201 *040101
Yucatan 4b.5(x) *0401 *040201 *0401 *0501 *0201
5.6 (w) *0401 *w08sw01 *05sw01 *0501 *0801
6.7 (y) *08sy01 *05sy01 *0601 *0601 *0601
7.8 (z) *0801 *0502 *0701 *0801 *0202
Westran 8.9 *02we02, *04we01 *07we01 *0302 *0201 *0402we01
Sinclair 9.10 (a) *0601 *0601 *0501 *0401 *0801
10.11 (c) *0501 *0302 *hm22 *0901 *0402
11.12 (d) *0101, *w09sm09 *0501 *0701sm19 *0602 *0701 Hanford 12.13 (e) *08sm08, *w09sm09 *10sm01 *0701sm19 *0403 *0303
13.0 (f) *w10sm21 *w13sm20 *0401 NT NT
㪈㩷䈚䈢䉪䊤䉡䊮䊚䊆䊑䉺䇮䊂䊠䊨䉾䉪⒳䊑䉺䇮䈍䉋䈶ႎ๔䈘䉏䈩䈇䉎㪪㪣㪘࿕ቯ䊑䉺䈱䊊䊒䊨䉺䉟䊒
Ꮕ䊊䊒䊨䉺䉟䊒
䉪䊤䉴Iㆮવሶ 䉪䊤䉴IIㆮવሶ 䊊䊒䊨䉺䉟䊒: Hp-
ຠ⒳ (ᣥฬ⒓) SLA-1 SLA-2 SLA-3 DRB1 DQB1
Clawn 16.16 (c1) *0401 *w09an02 *0602 *11ac21 *0601
17.17 (c2) null? *06an03 *03an02 *0801 *0501
16.17(c3) *0401 *w09an02 *0602 *0801 *0501 17.16(c4) null? *06an03 *03an02 *11ac21 *0601
Duroc 27.30 (d1) *08an03, *06an04 *0102 *0101 *1101 *0503
60.13 (d2) *-an02 *1002 *0502 *0403 *0303
Large White 1.1 (H01) *0101 *0101 *0101 *0101 *0101
NIH (MGH) 2.2 (a) *0201, *0701 *0201 null *0201 *0201
3.3 (c) null *0301 *0301 *0301 *0301
4a.4 (d) *0401 *0401 *0401 *0201 *040101
Yucatan 4b.5(x) *0401 *040201 *0401 *0501 *0201
5.6 (w) *0401 *w08sw01 *05sw01 *0501 *0801
6.7 (y) *08sy01 *05sy01 *0601 *0601 *0601
7.8 (z) *0801 *0502 *0701 *0801 *0202
Westran 8.9 *02we02, *04we01 *07we01 *0302 *0201 *0402we01
Sinclair 9.10 (a) *0601 *0601 *0501 *0401 *0801
10.11 (c) *0501 *0302 *hm22 *0901 *0402
11.12 (d) *0101, *w09sm09 *0501 *0701sm19 *0602 *0701 Hanford 12.13 (e) *08sm08, *w09sm09 *10sm01 *0701sm19 *0403 *0303
13.0 (f) *w10sm21 *w13sm20 *0401 NT NT
㪈㩷䈚䈢䉪䊤䉡䊮䊚䊆䊑䉺䇮䊂䊠䊨䉾䉪⒳䊑䉺䇮䈍䉋䈶ႎ๔䈘䉏䈩䈇䉎㪪㪣㪘࿕ቯ䊑䉺䈱䊊䊒䊨䉺䉟䊒
Ꮕ䊊䊒䊨䉺䉟䊒
䉪䊤䉴Iㆮવሶ 䉪䊤䉴IIㆮવሶ 䊊䊒䊨䉺䉟䊒: Hp-
ᩭ⒖ᬀ↪ෆᲣ⚦⢩ߩಓ⚿ሽᛛⴚߩㅴዷ
㧔␠࿅ᴺੱ⇓↥ᛛⴚදળ ෳਈ ⧎↰ ┨㧕
ᐔᚑ9㨪10ᐕᐲᆔ⸤ޟᩭ⒖ᬀߦ↪ࠆࠪࡇࠛࡦ࠻ෆᲣ⚦⢩ߩಓ⚿ᛛⴚ㐿⊒ޠ㧔ାᎺᄢቇ ᢎ㧕
㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ
ࠢࡠࡦ(ᩭ⒖ᬀ)ᛛⴚߪ㒰ᩭಣℂߣᵴᕈൻಣℂࠍᣉߒߚᚑᾫෆᲣ⚦⢩ߩ⺞ࠍᔅⷐߣ ߒޔߎߩࠪࡇࠛࡦ࠻ෆᲣ⚦⢩ߣ࠼࠽⚦⢩ߣࠍ㔚᳇⊛ߦⲢวߐߖߚౣ᭴▽⢦ߩ⒖ᬀߦࠃߞ ߡޔࠢࡠࡦࠍߔࠆޕߘߩ㓙ޔࠪࡇࠛࡦ࠻ෆᲣ⚦⢩ߩಓ⚿ሽ߇น⢻ߦߥࠇ߫ޔ
࠼࠽⚦⢩ߩ↪ᕈ߇ะߒޔࠢࡠࡦᛛⴚߩ᥉ߦ৻ጀᜉゞ߇߆߆ࠆ߽ߩߣᦼᓙߐࠇ ࠆޕᧄ⎇ⓥߢߪ࠙ࠪߩᩭ⒖ᬀߦ↪ࠆࠪࡇࠛࡦ࠻ෆᲣ⚦⢩ߩಓ⚿ሽᛛⴚࠍ⏕┙ߔࠆߎߣ ࠍ⋡⊛ߦޔ㧝㧕ൻෆᲣ⚦⢩ߦㆡߒߚಓ⚿Ⲣ⸃᧦ઙࠍᒻᘒᱜᏱᕈߦၮߠߡቯߒޔᰴߢ 㧞㧕ಓ⚿ಣℂߩᤨᦼࠍᦨㆡൻߔࠆߚߩᩭ⒖ᬀታ㛎ࠍⴕߞߚޕ
ታ㛎㧝㧕ੑᲑ㓏ಓ⚿ᴺߣࠟࠬൻሽᴺߩߕࠇߩૐ᷷ሽᣇᴺߦ߅ߡ߽ޔࠛ࠴ࡦ
ࠣࠦ࡞ߩಓኂ⼔ലᨐ߇߽ߞߣ߽ఝࠇߡ߅ࠅޔࠪࡘࠢࡠࠬߩᷝടߪߐࠄߦലߥࠃ߁ ߢߞߚޕ߹ߚޔෆᲣ⚦⢩ߩⲢ⸃ᓟߩᒻᘒᱜᏱ₸ߪࠟࠬൻሽᴺࠃࠅ߽ੑᲑ㓏ಓ⚿ᴺߩ߶
߁߇㜞߆ߞߚޕ
ታ㛎㧞㧕ೋᦼಽഀߪߔߴߡߩಓ⚿⟲ߢⷰኤߐࠇ(38㨪73%)ޔ⊒↢ㅦᐲߣ᭴ᚑ⚦⢩ᢙߦ㑐ߒߡ ኻᾖ⟲ߣᏅߩߥ⢦⋚⢩߇ᓧࠄࠇߚޕߒ߆ߒޔ⊒↢₸ߪࠢࡠࡦฃ♖ෆᒰߚࠅߢ2㨪6%ޔኻ ᾖ⟲ߩ୯߆ࠄߺࠆߣ5㨪14%ߦㆊ߉ߥ߆ߞߚޕ
⚿⺰ߣߒߡޔ㒰ᩭᓟߩ࠙ࠪෆᲣ⚦⢩ࠍࠛ࠴ࡦࠣࠦ࡞ߣࠪࡘࠢࡠࠬߩਔᣇࠍ⼔‛
⾰ߣߒߡੑᲑ㓏ಓ⚿ߒߡ߆ࠄᩭ⒖ᬀߔࠆߎߣߦࠃࠅޔ⢦⋚⢩߳ߩ⊒↢߇ᓧࠄࠇࠆߎߣޔ߹
ߚޔߘߩಓ⚿ሽߩ࠲ࠗࡒࡦࠣߪࠢࡠࡦ⢦ߩല₸ߦᓇ㗀ࠍ߷ߔⷐ࿃ߩ߭ߣߟߢࠆ ߎߣ߇ࠄ߆ߦߥߞߚޕ
㧞㧚⎇ⓥ㆐ߩ⁁
ಓ⚿ሽߒߚ࠙ࠪෆᲣ⚦⢩ߦᩭ⒖ᬀࠍᣉߒߡ߽ޔฃ⢦‐ߦ⒖ᬀน⢻ߥ⢦⋚⢩ᦼ߳ߩ⊒↢₸
ߪ㕖Ᏹߦૐߣႎ๔ߐࠇߡ߅ࠅޔᧄ⎇ⓥߢ߽ಓ⚿Ⲣ⸃ߩᓇ㗀ߪ⢦⋚⢩߳ߩ⊒↢ߩㆊ⒟ߢ߽ߞ ߣ߽㗼⪺ߦࠇߚޕ⎇ⓥታᣉᒰᤨޔෆᲣ⚦⢩ߩಓ⚿ሽߪ࿎㔍ߣ⠨߃ࠄࠇߡ߅ࠅޔߘߩὐࠍ
⠨ᘦߔࠇ߫వ㚟⊛⎇ⓥߢߪߞߚ߇ޔᓧࠄࠇߚ⚿ᨐߩ߹߹ߢߪ⇓↥႐ߦᵴ↪ߢ߈ࠆߣߪ
߃ߥ߆ߞߚޕ
ߒ߆ߒޔᧄ⎇ⓥߪᒰಽ㊁ߩ⎇ⓥ⠪ࠍೝỗߒޔߘߩᓟߩૐ᷷↢‛ቇߣಓ⚿ሽᛛⴚߩᔀᐩ
⊛ߥㅴዷߦࠃߞߡᛛⴚߩ⏕┙ࠍࠆߦ⥋ߞߚޕߎࠇߪᕆㅦࠟࠬൻሽᴺߣࠊࠇࠆ߽ߩ ߢޔᓥ᧪ߩࠟࠬൻሽᴺߩ಄ළ́Ⲣ⸃ㅦᐲࠍᡷༀߒߚᣇᴺߢࠆޕࠟࠬൻሽߣߪޔ㜞 Ớᐲߩಓ⚿⼔‛⾰ࠍᷝടߒߚṁᶧߦࠃߞߡ⹜ᢱ⚦⢩ࠍචಽ⣕᳓ߒޔ⚦⢩ౝᄖߢߩ᳖᥏ᒻᚑ ࠍ㒐߉ޔࠟࠬൻ⁁ᘒߢᶧ⓸⚛ਛߦሽߔࠆᣇᴺߢࠆޕᧄ⎇ⓥߣห᭽ߦޔಓ⚿⼔‛⾰
ߦߪ⚦⢩߳ߩㅘㆊᕈ߇㜞ߊޔᲥᕈ߇ዋߥࠛ࠴ࡦࠣࠦ࡞ߣ⣕᳓ଦㅴߩߚߩࠪࡘࠢࡠ
ࠬ(㕖ㅘㆊᕈ)ࠍ⚵ߺวࠊߖߚṁᶧࠍ↪ߔࠆޕ
㗴ߪෆᲣ⚦⢩ߩኈེߢޔᧄ⎇ⓥߢߪࠬ࠻ࡠࠍ↪ߒߚ߇ޔን᳗ࠄ(2001)ߪᏒ⽼ߩ࠴࠶
ࡊࠍᵹ↪ߒߚࠥ࡞ࡠ࠺ࠖࡦࠣ࠴࠶ࡊᴺࠍ㐿⊒ߒߚ(౮⌀)ޕ
ߘߩὐߪࠝࡊࡦߥኈེߩߚಓ⚿ᤨߩ‛ℂ⊛ജࠍ㒐߉ޔߏߊዋ㊂ߩᶧ(0.6ǴͰ)ߣߦ
ෆᲣ⚦⢩ࠍᕆㅦ಄ළߔࠆߎߣߦࠆޕห᳁ࠄ(2005)ߪߎߩሽෆᲣ⚦⢩߇ޔⲢ⸃ᓟޔ95%
߇ᒻᘒ⊛ߦᱜᏱߢޔ㒰ᩭ₸88%ޔᝌߒߚෆਐ⚦⢩ߣߩⲢว₸79%ޔෆഀ₸70%ޔ⢦⋚⢩⊒
↢₸45%ߣ߁㜞ᚑഞ₸ࠍႎ๔ߒߚޕ
㧟㧚ᛛⴚታ↪ൻߩ⁁
ㅀߩࠃ߁ߦᧄᛛⴚߪߔߢߦቢᚑߒߡ߅ࠅޔ࠙ࠪߩߺߥࠄߕૐ᷷ᗵฃᕈߩ㜞ࡉ࠲ࠍ
ߚઁߩູ㘃ߩෆᲣ⚦⢩߿⢦ߩሽߦ߽ലߢࠆޕࡅ࠻ෆᲣ⚦⢩ߢߪ⚂ 100%ߩᚑഞ₸ߢ
ࠅޔ㗼ᓸ♖ߣߩ⚵ߺวࠊߖߦࠃࠆਇᅧᴦ≮ߦᔅ㗇ߩᛛⴚߣߒߡ⇇⊛ߦᵴ↪ߐࠇߡࠆ (᪀ጊߩࠢࠗࠝ࠻࠶ࡊᴺޔ2005)ޕ
㧠㧚ᓟߩᣇะޔ⺖㗴
㐿⊒⾗㊄߳ߩᡰេⷐ⺧ߪᄙޕߘߩ႐วޔ⎇ⓥ⺖㗴ౝኈߩክᩏߩߺߥࠄߕޔቯᓟߩ⎇ⓥ
ㅴ⁁ᴫߣ⚳ੌᓟߩㅴዷ⁁ᴫߩᛠី߇ࠃࠅ㊀ⷐߢࠆޕ 㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥႎ๔╬
㧝㧕Tominaga K., Hamada Y., Hochi S.㧦Gel-loading tip vitrification of in vitro-matured bovine oocytes and subsequent embryo production by IVF and nuclear transfer.
J.Mamm.Ova Res.22, 178-184, 2005.
㧞㧕Kuwayama M.㧦Oocyte cryopreservation.( Mini Review) J.Mamm.Ova Res.24,2-7, 2007.
౮⌀㧦() ࠥ࡞ࡠ࠺ࠖࡦࠣ࠴࠶ࡊ㧦ෆᲣ⚦⢩ߣಓ⚿ᶧࠍๆᒁߒޔ ᶧ⓸⚛ਛߦᶐߔޕ
(ਅ) 0.5 mlࠬ࠻ࡠߦⵝ⌕ߒޔᶧ⓸⚛ਛߦሽߔࠆޕ
⇓↥♽ឃ᳓ߩࡔ࠲ࡦ⊒㉂ಣℂᛛⴚߣ⊒㉂ᶧߩ⢈ᢱォ↪ᛛⴚߩዷᦸ
㧔᧲ർᄢቇᄢቇ㒮Ꮏቇ⎇ⓥ⑼ ᢎ ේ↰⑲᮸㧕
ᐔᚑ10㨪11ᐕᐲᆔ⸤ޟᄙᲑᑼUASBࡊࡠࠬߦࠃࠆ⇓↥ឃ᳓ߩ㜞᷷ࡔ࠲ࡦ⊒㉂ಣℂߣMAPᨆᴺߦࠃࠆⴡ
↢ቇ⊛ߦోߥ⢈ᢱ↢↥ᛛⴚߩ⏕┙ޠ㧔㐳ጟᛛⴚ⑼ቇᄢቇ ᢎ㧕
㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ
⇓↥ᑄ᫈‛ߪޔ⾆㊀ߥᯏ⾰⾗Ḯߣߒߡޔߎࠇ߹ߢㄘ↥‛߿㘺ᢱ‛ߩ↢↥ߦലߦ↪
ߐࠇߡ߈ߚ߇ޔ⇓↥⚻༡ߦ߅ߌࠆᕆㅦߥⷙᮨᄢ╬ߦࠃࠅឃߖߟ‛ߩ⊒↢㊂߇Ⴧᄢߒޔ⥄Ꮖ
⚻༡ౝࠆߪၞౝߦ߅ߌࠆല↪߇࿎㔍ߦߥߞߡ߈ߡࠆޕߐࠄߦኅ⇓ឃߖߟ‛ᴺߩ ቢోᣉⴕߦࠃࠅޔߘߩಣℂߪ߽ߣࠃࠅޔㆡᱜߥ▤ℂ↪ߩଦㅴ߇ᒝߊ᳞ࠄࠇߡࠆޕ
ߘߎߢᧄ⎇ⓥߢߪޔ⇓↥♽ឃ᳓ߩല₸⊛ߥಣℂߣ㜞ല₸ࠛࡀ࡞ࠡ࿁ࠍ⋡⊛ߣߒߡޔᧄ
⎇ⓥቶߢ㐿⊒ߒߚᄙᲑᑼUASBࡊࡠࠬࠍㆡ↪ߒޔߘߩലᕈߦߟߡᬌ⸛ߒߚޕ߹ߚ⇓↥
♽ឃ᳓߆ࠄߩ⢈ᢱᚑಽ(⓸⚛㨯ࡦ)ߩ࿁ࠍ⹜ߺࠆߚߦޔ⽋⥢ឃ᳓ߩUASBಣℂ᳓ࠍ↪ߡޔ MAPᨆᴺߩ⻉᧦ઙߦߟߡߩၮ␆⊛ᬌ⸛ࠍⴕߞߚޕ
㧞㧚⎇ⓥ㆐ߩ⁁
㧝㧕ᄙᲑဳ UASB ࠕࠢ࠲ߩㅪ
⛯ಣℂታ㛎㧦
࿑㧝ߦ␜ߔᄙᲑᑼ UASB ࠍ↪ޔ CODỚᐲࠍ⚂21,600mg-COD/lޔSS Ớᐲࠍ⚂ 12,800mg/lߦ⺞ᢛߒߚឃ᳓
ࠍޔᓴⅣᲧ 10㨪20 ߢಣℂߒߚ⚿ᨐޔ ኈ Ⓧ ⽶ ⩄ 4.0㨪5.4 kg-COD m-3 d-1(HRT ⚂ 5 ᣣ)ߢޔో COD ߅ࠃ߮
ṁ⸃ᕈ㒰₸ߣ߽ߦ 85%એޔో
VFAỚᐲ250mg-COD/lએਅޔࡔ࠲ࡦ ォ឵₸ 70㧑⒟ᐲߢㆇォน⢻ߢߞߚޕ
㧞㧕ህ᳇ᕈಣℂ᳓ߦ߅ߌࠆMAP↢ ᚑ᧦ઙ╬ߩᬌ⸛㧦
Mg/Pࡕ࡞Ყࠍ1.3ߦ⸳ቯߒpH8㨪9 ߩ▸࿐ߢህ᳇ᕈಣℂ᳓ߩ MAPᨆታ 㛎ࠍⴕߞߚ⚿ᨐࠍ࿑㧞ߦ␜ߔޕࡦߦ 㑐ߒߡߪޔpH10 ߦ߅ߡᦨ߽ఝࠇߚ 㒰₸߇␜ߐࠇߚ߇ޔࠕࡦࡕ࠾ࠕߩ㒰
₸ߪ20%⒟ᐲߢߞߚޕ↢ᚑߐࠇߚ
⚿᥏ߦߟߡ EDX ߢቯᕈಽᨆࠍⴕߞ ߚߣߎࠈޔPߣMgએᄖߦCaߩሽ
߇⏕ߐࠇߚޕ߹ߚޔ⚿᥏ߩX✢࿁᛬
ߦࠃࠅޔMAPએᄖߦࡅ࠼ࡠࠠࠪࠕࡄࠨࠗ࠻(HAP)ߩሽ߇⏕ߢ߈ߚޕHAPߩ↢ᚑߪMAP
ᔕᤨߦࠕࡦࡕ࠾ࠕ㒰ࠍ㒖ኂߔࠆߎߣ߆ࠄޔᓟߩኻ╷߇ᔅⷐߢࠆߣ⠨߃ࠄࠇߚޕߥ߅ޔ SS߇߹ࠇߡߡ߽ࡦߩ㒰₸ߦᄢ߈ߥᄌൻߪߺࠄࠇߥ߆ߞߚޕ
࿑㧝 ᄙᲑᑼUASBࠕࠢ࠲
0 20 40 60 80 100
7 8 9 10 11
pH
Removal(%)
PO4-P PO4-P(SS) NH4-N NH4-N(SS)
࿑㧞 MAPᨆᴺߩ⚿ᨐ
㧟㧚ᛛⴚታ↪ൻߩ⁁
ޔ⇓↥ឃ᳓ߩಣℂᣇᴺߣߒߡ UASB ᴺ߇ᵈ⋡ࠍᶎ߮ޔߘߩㆡ↪߽Ⴧ߃ߟߟࠆޕ ߒ߆ߒߥ߇ࠄޔ߹ߛᔕᮏౝߢߩ SS ߩ⫾Ⓧ╬ߩ㗴ࠍᛴ߃ߡࠆߩ߇⁁ߢࠈ߁ޕᧄ
⎇ⓥߢឭ໒ߒߚᄙᲑဳUASBߦ߅ߡ߽ޔଐὼSSߩ⫾Ⓧߪߺࠄࠇࠆ߽ߩߩޔᓥ᧪ߩUASB ᴺࠃࠅ߽࿕ᒻᚑಽߩ㒰นṁൻଦㅴࠍታߒߡ߅ࠅޔߘߩ㗴ὐࠍ⸃ߔࠆߚߩᛛⴚะ
ߦᄢ߈ߊነਈߒߚߣ⠨߃ࠄࠇࠆޕᄙᲑဳUASBߦࠃࠆឃ᳓ಣℂᛛⴚߪޔ⇓↥ឃ᳓એᄖߦ߽
ᄙߊߩ㘩ຠㅧ♽ߩឃ᳓ಣℂߦㆡ↪ߐࠇޔޔ㐿⊒ߩᦨ⚳Ბ㓏ߦࠆߣ߃ࠆޕ
߹ߚޔMAPᨆᛛⴚߪޔMAPઃ⌕ᶐẃ᧚ᢱࠍᛩߒߚࠕࠢ࠲ߥߤ߇㐿⊒ߐࠇޔ⎇ⓥ
↳⺧ᒰᤨࠃࠅ߽ޔ⇓↥ឃ᳓ߩಣℂ߅ࠃ߮ଔ‛࿁ᛛⴚߣߒߡޔᐢߊ᥉ߒߟߟࠆࠃ߁ߢ
ࠆޕ
㧠㧚ᓟߩᣇะ⺖㗴
ᚒޘߩ⎇ⓥࠣ࡞ࡊߢߪޔᧄᬺ⚳ੌᓟ߽ޔᒁ߈⛯߈⇓↥ឃ᳓ᑄ᫈‛㑐ଥߩࡔ࠲ࡦ⊒㉂
ಣℂߣ⊒㉂ᶧߩ⢈ᢱ↪╬ߩ⎇ⓥࠍㅴߡࠆޕ
⇓↥ᑄ᫈‛ߩࡔ࠲ࡦ⊒㉂ߩࡂ࠼⊛⺖㗴ߢࠆ࿕ᒻಽߩนṁൻߩଦㅴߦߟߡߪޔᧄᬺ
ߦ߅ߌࠆ⍮ࠍ߽ߣߦޔࡄࡓࠝࠗ࡞ㅧᑄᶧߩࠃ߁ߥޔࠃࠅ㜞Ớᐲ㜞 SS ឃ᳓ಣℂ
߳ߣߟߥ߇ࠅޔࡑࠪࠕߩ↢↥႐ߢߩታ↪ൻ⹜㛎ߥߤࠍⴕߞߡࠆޕ߹ߚ࿕ᒻᑄ᫈‛ߩ Ḩᑼ߅ࠃ߮ੇᑼࡔ࠲ࡦ⊒㉂ಣℂᛛⴚߦߟߡ߽ᛛⴚᬌ⸽ࠍⴕߞߡࠆޕߎߩࠃ߁ߦޔࠃࠅ㜞 SSឃ᳓ߩಣℂࠆߪ࿕ᒻ‛ߩല₸⊛ᶖൻࡔ࠲ࡦ⊒㉂ᛛⴚߩ⏕┙߇ޔᓟߩ⺖㗴ߣߒ ߡᒝߊᦸ߹ࠇߡࠆޕ
ߐࠄߦޔ⇓↥♽ᑄ᫈‛ߩ⊒㉂ᶧᱷᷲࠍ⢈ᢱߣߒߡല↪ߔࠆ⠨߃ᣇߪޔᓟ߽㊀ⷐߥ⺖
㗴ߢࠈ߁ޕᚒޘ߽ޔታⷙᮨߩ‐♽⇓↥ᑄ᫈‛ߩࡔ࠲ࡦ⊒㉂ࡊࡦ࠻ߦߟߡޔ⊒㉂ᶧࠍ
⋥ធᣉ⢈ߔࠆߎߣࠍᔨ㗡ߦޔߘߩല₸⊛ᶖᲥᚻᴺߩ㐿⊒ߣ⢈ᢱߩⴡ↢ቇ⊛ోᕈ⹏ଔᚻᴺߩ
⏕┙╬ࠍ⋡ᜰߒߡࠆ(Uemura S., Harada H.ઁ:Production of biologically safe digested manure for land application by a full-scale biogas plant with heat-inactivation. Waste Management and Research, 26, 247-255㗁, 2008.㧕ޕ
㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥႎ๔╬
㧝㧕❥᮸ޔේ↰⑲᮸ઁ㧦ᄙᲑဳUASBᔕེߦࠃࠆ㜞ỚᐲSSឃ᳓ߩಣℂ․ᕈޔ
↪᳓ߣᑄ᳓ vol.43, No.11, 961-967, 2001.
㧞㧕Uemura S., Harada H.ઁ㧦Treatment of wastewater containing high amount of suspended solids by a novel multi-staged UASB reactor. Environmental Technology, vol.26, 1355-1362, 2005.
࠙ࠪෆᲣ⚦⢩ߩᄖᚑᾫၭ㙃ᦼ㑆ߦ₪ᓧߔࠆ⢦⊒↢⢻ߦ߷ߔࡒ࠶
࠼ࠞࠗࡦߩലᨐߦ㑐ߔࠆ⎇ⓥߩㅴዷߣታ↪ൻ߳ߩዷᦸ
㧔੩ㇺᄢቇ ಎᢎ ጊ↰㓷㧕
ᐔᚑ10㨪11ᐕᐲᆔ⸤ޟູേ‛ෆሶߩᚑᾫ߅ࠃ߮⢦⊒↢ߦኻߔࠆᯏ⢻⊛ၭ㙃ᴺߩ㐿⊒ޠ㧔੩ㇺᄢቇ ഥᢎ㧕 ᐔᚑ12㨪13ᐕᐲᆔ⸤ޟ࠙ࠪෆሶ⚦⢩⾰ᚑᾫߩଦㅴߦ㑐ࠊࠆಽሶၮ⋚ޠ㧔੩ㇺᄢቇ ഥᢎ㧕
㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ
࠙ࠪߩല₸⊛Ⴧ↥ߦ߅ߡޔዼࠆߪ↢ౝߩෆᎽෆ⢩߆ࠄណขߒߚᧂᚑᾫෆᲣ⚦⢩
ࠍᄖᚑᾫၭ㙃ߦࠃߞߡฃ♖น⢻ߥ⁁ᘒ߹ߢᚑᾫߐߖߚᓟߦޔᄖฃ♖߅ࠃ߮ᄖၭ㙃ߦࠃ ߞߡ⒖ᬀน⢻ߥ⢦⋚⢩ࠍ↢↥ߔࠆ৻ㅪߩၭ㙃ᛛⴚ߇㐿⊒ߐࠇޔല↪ߐࠇߡࠆޕߒ߆ߒޔ ᓥ᧪ߩࠃ߁ߥᄖၭ㙃ᛛⴚߦࠃߞߡෆᲣ⚦⢩߆ࠄߐࠇࠆ⢦⋚⢩ߩഀวߪޔ↢ౝ↱᧪⢦
ߦᲧߴߡࠄ߆ߦૐߎߣ߇㗴ߣߥߞߡࠆޕෆᲣ⚦⢩ߩᄖၭ㙃ߦࠃࠆ⢦↢↥ߩߐࠄߥ ࠆല₸ൻࠍ࿑ࠆߚߦߪޔ⢦⋚⢩ᦼ߳⊒↢ߔࠆ⢦ߩ⢻ജ߇ฃ♖೨ߩෆᲣ⚦⢩ߩ⚦⢩⾰ᚑᾫ(ෆ
⚦⢩⾰ᚑᾫ)ߦࠃߞߡਥߦ₪ᓧߐࠇࠆߎߣ߆ࠄޔ࠙ࠪෆᲣ⚦⢩ߩᄖᚑᾫၭ㙃ᛛⴚߩᡷༀ߇ᭂ
ߡ㊀ⷐߣߥࠆޕ
ෆᲣ⚦⢩ߩᚑᾫߪޔᕈ⣼ೝỗࡎ࡞ࡕࡦߛߌߢߥߊޔ㗰☸ጀ⚦⢩ޔෆ⢩ᶧ߅ࠃ߮ෆਐ⚦⢩ෆ Უ⚦⢩ⶄว(COC)߆ࠄ᭴ᚑߐࠇࠆෆ⢩ౝߩዪᚲᕈ࿃ሶߦࠃߞߡ⺞▵ߐࠇߡࠆޕᚒޘߪޔ ෆ⢩ᶧਛߦሽߔࠆᣂⷙࡋࡄࡦ⚿วᕈᚑ㐳࿃ሶߢࠆࡒ࠶࠼ࠞࠗࡦ(MK)߇࠙ࠪෆ⚦⢩⾰
ᚑᾫଦㅴ࿃ሶߢࠆߎߣޔ߅ࠃ߮ߘߩ↪ᯏᐨࠍએਅߩ⎇ⓥ߆ࠄࠄ߆ߦߒߚޕ
߹ߕޔ࠙ࠪ MK(bMK)cDNA ߩࠢࡠ࠾ࡦࠣߣࠦࡦࡆ࠽ࡦ࠻ bMK ߩࠍ⹜ߺߚޕߘ ߩ⚿ᨐޔbMK cDNA߆ࠄ੍ᗐߐࠇࠆbMK(ಽሶ㊂⚂16kDa)ߩࠕࡒࡁ㉄㈩ߪޔࡑ࠙ࠬ߅ࠃ
߮ࡅ࠻ MKߣ㜞⋧หᕈࠍ␜ߒޔ10ߩࠪࠬ࠹ࠗࡦᱷၮ߇࠙ࠪޔࡑ࠙ࠬޔࡅ࠻ߩ㧟⒳㑆ߢޔ 30 ߩႮၮᕈࠕࡒࡁ㉄ᱷၮ߇ࡅ࠻ߣ࠙ࠪߩ㑆ߢሽߐࠇߡࠆ(࿑㧝)ߎߣޔߐࠄߦޔ⯻⚦
⢩⊒♽ߦࠃߞߡࠦࡦࡆ࠽ࡦ࠻bMKࠍ㜞₸ߢߢ߈ࠆߎߣ߇ࠊ߆ߞߚޕ
ᰴߦޔߒߚࠦࡦࡆ࠽ࡦ࠻ bMK ߩ࠙ࠪෆ⚦⢩⾰ᚑᾫߦ߷ߔലᨐࠍޔᄖฃ♖ᓟߩ ෆഀ߅ࠃ߮⢦⋚⢩߳ߩ⊒↢₸ߦࠃࠅᬌ⸛ߒߚޕbMK(200ng/ml)ࠍᷝടߒߚᚑᾫၭߢ࠙ࠪ
COCࠍၭ㙃ߔࠆߣޔᩭߩᚑᾫߣᄖฃ♖ᓟߩෆഀߪᓇ㗀ࠍฃߌߥ߇ޔ⢦⋚⢩߳ߩ⊒↢₸߇ ήಣℂኻᾖߦᲧߴߡᗧߦ㜞ߊߥߞߚ(ಣℂ:50%ޔήಣℂ 21%ޔP<0.05㧕ޕ߹ߚޔCOC ߆ࠄෆਐ⚦⢩ࠍ㒰ߒߚൻෆᲣ⚦⢩(DO)ࠍbMKᷝടᚑᾫၭߢၭ㙃ߒߡ߽ޔᄖฃ♖ᓟޔ
⢦⋚⢩߳ߪోߊ⊒↢ߒߥ߆ߞߚ߇ޔෆਐ⚦⢩ߣߩၭ㙃ߢᄖᚑᾫࠍⴕߞߚ႐วޔbMKߦࠃ ࠅDOߩ⢦⋚⢩߳ߩ⊒↢߇ଦㅴߐࠇߚޕߐࠄߦޔbMKᷝടࠆߪήᷝടၭߢၭ㙃ߒߚෆ ਐ⚦⢩ߩ㚔ൻၭ(CMMK+ޔCMMK-)ࠍ⺞ᢛߒޔDOߩ⚦⢩⾰ᚑᾫߦ߷ߔߘࠇߙࠇߩ㚔ൻ
ၭߩലᨐࠍᬌ⸛ߒߚ⚿ᨐޔDOߩ⚦⢩⾰ᚑᾫଦㅴᵴᕈߪ CMMK+ߦߩߺሽߒޔߘߩᵴᕈ ߪᾲ(100͠)߅ࠃ߮࠻ࡊࠪࡦಣℂߦࠃߞߡᄬᵴߒߚޕ
એߩ⚿ᨐ߆ࠄޔbMKߩෆ⚦⢩⾰ᚑᾫଦㅴലᨐߪޔෆਐ⚦⢩߆ࠄಽᴲߐࠇࠆࡐࡍࡊ࠴࠼
ᕈߩᶧᕈ࿃ሶࠍߒߡ⊒ើߐࠇࠆߎߣ߇␜ໂߐࠇߚޕ߹ߚޔCOCߩᄖᚑᾫၭ㙃ᦼ㑆ߦޔෆ ਐ⚦⢩ߩࠕࡐ࠻ࠪࠬᄌᕈ߇ߎࠆߎߣ߇⍮ࠄࠇߡࠆ߇ޔbMKಣℂߦࠃࠅࠕࡐ࠻ࠪࠬᄌ ᕈߪᛥߐࠇࠆߎߣ߇ޔTunnelᨴ⦡߅ࠃ߮LM-PCRᴺߦࠃߞߡࠄ߆ߣߥߞߚ2)ޕᓥߞߡޔ bMKߪෆਐ⚦⢩ߩ↢ሽᕈߣᯏ⢻ޔߟ߹ࠅෆ⚦⢩⾰ᚑᾫଦㅴ࿃ሶߩಽᴲᯏ⢻ࠍ㜞ࠆߎߣߦࠃ ߞߡޔ࠙ࠪෆᲣ⚦⢩ߩ⚦⢩⾰ᚑᾫߦኻߔࠆଦㅴലᨐࠍ⊒ើߒߡࠆߣ⠨߃ࠄࠇߚޕ
㧞㧚⎇ⓥ㆐ߩ⁁
ෆᲣ⚦⢩ߩ⚦⢩⾰ᚑᾫଦㅴߦ߅ߡޔෆਐ⚦⢩ߣߩ⋧↪߇㊀ⷐߥᓎഀࠍᨐߚߒߡࠆ ߇ޔߘߩ⋧↪ߦ㑐ߔࠆ⎇ⓥߪޔ࿖ౝߢߪ✜ߦዞߚ߫߆ࠅߢࠆޕᚒޘߩ⎇ⓥߢߪޔ
ࠦࡦࡆ࠽ࡦ࠻ bMKࠍ⯻⚦⢩⊒♽ߢᄢ㊂ߦߔࠆߎߣ߇น⢻ߣߥߞߚߎߣ߆ࠄޔbMK ߩ࠙ࠪෆ⚦⢩⾰ᚑᾫଦㅴᯏ᭴ߩ⸃ߦ㑐ߔࠆ⎇ⓥ߇ㅴዷߒޔbMKߩ↪ࠍฃߌߚෆਐ⚦⢩߆ ࠄಽᴲߐࠇࠆෆ⚦⢩⾰ᚑᾫଦㅴ࿃ሶߩត⚝ࠍ㐿ᆎߒߡࠆޕbMKಣℂߦࠃߞߡෆਐ⚦⢩ߢࠃ ࠅᒝߊ⊒ߔࠆㆮવሶࠍࠨࡉ࠻࡚ࠢࠪࡦᴺߢษࠅߍࠆߎߣࠍ⹜ߺޔ5 ߟߩㆮવሶࠍหቯ ߒߚޕᓟޔߎࠇࠄߩㆮવሶ↥‛ߣෆ⚦⢩⾰ᚑᾫଦㅴᵴᕈߣߩ㑐ଥࠍᬌ⸛ߔࠆ੍ቯߢࠆޕ 㧟㧚ᛛⴚታ↪ൻߩ⁁
bMKᷝടၭࠍ↪ߚ࠙ࠪCOCߩᄖᚑᾫၭ㙃♽߇㐿⊒ߐࠇߚߎߣߦࠃߞߡޔ࠙ࠪᄖ ฃ♖⢦↢↥ᛛⴚߪᡷༀߐࠇߚޕߒ߆ߒޔDO߆ࠄߩ⢦↢↥ߦ߅ߡߪޔbMKಣℂၭ㙃ෆਐ⚦
⢩ߩ㚔ൻၭਛߦ DOߩෆ⚦⢩⾰ᚑᾫࠍଦㅴߔࠆᵴᕈ࿃ሶ߇ሽߔࠆߎߣ߇⏕ߐࠇߡࠆ ߩߺߢޔߘߩᵴᕈ࿃ሶߪหቯߐࠇߡߥޕߎߩᵴᕈ࿃ሶ߇หቯߐࠇޔᣂⷙߩᄖᚑᾫၭ㙃 ᛛⴚ߇㐿⊒ߐࠇࠇ߫ޔෆᲣ⚦⢩࿐ߩෆਐ⚦⢩ߩઃ⌕⁁ᘒߦ߆߆ࠊࠄߕޔ߹ߢએߦല₸
⊛ߥෆᲣ⚦⢩߆ࠄߩ⢦↢↥߇น⢻ߣߥࠅޔ⇓↥ᬺ߫߆ࠅߢߥߊޔᄖᚑᾫෆᲣ⚦⢩ߩᄖฃ
♖߿㗼ᓸ♖ߦࠃࠆਇᅧᴦ≮ࠍⴕ߁↢ᱺක≮ಽ㊁ߦ߽⽸₂ߔࠆߎߣ߇ᦼᓙߐࠇࠆޕ 㧠㧚ᓟߩᣇะޔ⺖㗴
ෆᲣ⚦⢩ߩ⚦⢩⾰ᚑᾫߩ⺞▵ߦ߅ߡޔෆਐ⚦⢩ߣෆᲣ⚦⢩㑆ߩ⋧↪߇ᭂߡ㊀ⷐߥ ᓎഀࠍᨐߚߒߡࠆޕ․ߦޔෆਐ⚦⢩߆ࠄෆᲣ⚦⢩ߦ⋥ធ↪ߒߡ⚦⢩⾰ᚑᾫࠍଦㅴߔࠆᶧ ᕈ࿃ሶࠍන㔌หቯߔࠆߎߣ߇ޔCOCߩߺߥࠄߕൻෆᲣ⚦⢩߆ࠄ࠙ࠪ⢦ࠍ㜞ല₸⊛ߦ↢↥
ߔࠆᄖၭ㙃ᛛⴚߩ㐿⊒ߦ߅ߡޔᦨ߽㊀ⷐߥ⎇ⓥ⺖㗴ߣ⠨߃ࠄࠇࠆޕߎߩ⺖㗴ࠍ⸃ߔࠆ
ౕ╷ߣߒߡޔbMKಣℂၭ㙃ෆਐ⚦⢩ߩ㚔ൻၭߦሽߔࠆ࠙ࠪൻෆᲣ⚦⢩ߩ⚦⢩⾰ᚑᾫ ଦㅴ࿃ሶࠍන㔌หቯߔࠆ⎇ⓥ߇ߍࠄࠇࠆޕ
㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥႎ๔╬
㧝㧕Ikeda S., et al.㧦Mol. Reprod. Dev., 57: 99-107, 2000.
㧞㧕Ikeda S., et al.㧦Reproduction, 132: 549-557, 2006.
㪈 㪈㪌
㪊㪐
㪉㪊 㪊㪇 㪢 㪢 㪢
㪢 㪢 㪢 㪚
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㪚
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䉡䉲䊚䉾䊄䉦䉟䊮䈱৻ᰴ᭴ㅧ 㪩
㪛 㪞 㪞 㪧
㪘
㪭
㪠
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㪞 㪘 㪧
㪘
䊶㪊㪇䈱Ⴎၮᕈ䉝䊚䊉㉄䈲㪿㪤㪢䈫ห৻㩷㩿㩷㩷㩷㩷㩷㪀 䊶㪈㪇䈱䉲䉴䊁䉟䊮㩷㩿㩷㩷㩷㩷㩷㪀㩷
㪞 㪪 㪜
㪜 㪮 㪮
㪞 㪧 㪫 㪧 㪪
㪪 㪛 㪞 㪞 㪜 㪝
㪞 㪫
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㪘
㪛 㪞 㪞㪫 㪨 㪫
㪞 㪣 㪫 㪘
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㪘
㪨 㪨 㪜 㪫 㪠 㪭
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㪫 㪘
㪞 㪛
࿑㧝 ࠙ࠪࡒ࠶࠼ࠞࠗࡦߩ৻ᰴ᭴ㅧ
30ߩႮၮᕈࠕࡒࡁ㉄ߪࡅ࠻ࡒ࠶࠼ࠞࠗࡦߣห৻㧔 㧕
10ߩࠪࠬ࠹ࠗࡦ㧔 㧕
㘩ᢱ⥄⛎₸ะߩߚߩᨋ㑆’ࠪࠬ࠹ࡓߩౣ᭴▽
㧔᧲ർᄢቇᄢቇ㒮 ᢎ ⮮ⴐ㧕
ᐔᚑ12㨪13ᐕᐲᆔ⸤ޟૐࠦࠬ࠻ᄢⷙᮨᨋ㑆’ࠪࠬ࠹ࡓߩઍ⊛ౣ᭴▽ޠ㧔᧲ർᄢቇ ഥᢎ㧕
㧝㧚ᛛⴚߩ⢛᥊ߣ⎇ⓥߩౝኈ
㘩ᢱߩ⥄⛎₸ะߪޔㄘߩᦨᄢߩ⺖㗴ߢࠆޕ⇓↥ߦ߅ߡߪޔ㘺ᢱ⥄⛎₸ߩะ߇ߘ ߩ೨ឭߣߥࠆ߇ޔᧂߛߦ24㨪25%ߢផ⒖ߒߡ߅ࠅޔࠄࠁࠆ㘺ᢱ⥄⛎ࠪࠬ࠹ࡓߩឭ᩺߇ᦼᓙ ߐࠇߡࠆޕㄭᐕߩⓃ‛ଔᩰ㜞㛛ߪ㔛⛎㑐ଥߩ᭴ㅧ⊛㗴߆ࠄ⊒ߒߡ߅ࠅޔߎߩⷐ⺧ߪᧂߛ ߦᣣ⊛⺖㗴ߢࠆޕᧄ⎇ⓥߢߪޔᨋߩਅ⨲ߣߒߡ⼾ንߦሽߔࠆᬀ‛ࠍޔ‐ߩ㘺ᢱ⾗Ḯ ߣߒߡ↪ߔࠆࠪࠬ࠹ࡓߩ㐿⊒ࠍ⋡⊛ߣߒߚޕ
ᨋౝ’ߪᭂߡฎᱧผࠍᜬߟ߇ޔᴦ30ᐕઍߦㅧᨋᬺ߇㐿ᆎߐࠇࠆߎߣߦࠃࠅޔ
ᨋ߆ࠄ‐㚍ߪㅊߐࠇޔᄢᱜ5ᐕ(1916ᐕ)ߩ㚍↥ଏ↪㒢ቯᐲߩਛߢޔᨋౝ’ߪߐࠄ ߦ㒢ቯߐࠇࠆߎߣߣߥߞߚޕㄭᐕ(1998ᐕ)ߩ⺞ᩏߢߪޔ’↪ᨋ㊁(౮⌀㧝)ߪ1.6ਁhaߦ
߹ߢ⪭ߜㄟߺޔߐࠄߦᷫዋߩ৻ㅜࠍㄡߞߡࠆޕߒ߆ߒޔ⡺↪❥ᱺ‐ߩᩕ㙃ⷐ᳞㊂ߪૐߚ
ޔߘߩ㘺㙃ߦߣߞߡᨋߩਅ⨲ߪᧂߛߦ㝯ജ⊛ߢࠆޕ㉿ጊߢࠆᣥ⮌ᨋ߇ 618ਁhaޔ ᅏጊߢࠆ’↪ᨋ㊁߇1.6ਁhaߣߔࠆߣޔ⡺↪⒳❥ᱺ‐63.6ਁ㗡ߪో㗡ߎߎߢ㘺⢒ߢ ߈ࠆޕ‐⟲ߩ⋙ⷞࠍޔ⋙ⷞੱߦࠃࠆᾫ✵ߒߚᛛ⢻߆ࠄ⑼ቇ⊛࠺࠲ߦၮߠߊᛛⴚߦ᥉ㆉൻߒޔ ߆ߟઁߩ↢ᘒ♽ࠨࡆࠬ(↢‛ᄙ᭽ᕈߥߤߩᄙ㕙⊛ᯏ⢻)߽ᗧ⼂ߒߚᨋ㑆’ࠪࠬ࠹ࡓ㐿⊒߇ ᧄ⎇ⓥߩౝኈߢޔᬀᨋᐕ߇⇣ߥࠆ㊎⪲᮸ੱᎿᨋ⃰(ࠬࠡޔࠞࡑ࠷)ߣੱᎿᨋߩ㒐㘑↪ߣߒߡߘ ࠇࠄࠍ࿐߁ᐢ⪲᮸ᨋ⃰(ࡉ࠽ޔࡒ࠭࠽╬)߇ࡕࠩࠗࠢ⁁ߦሽߔࠆౖဳ⊛ߥㄭઍ⊛ᣉⴕᴺߩ
ᨋߢߩᚑᨐߢࠆޕ
㪇 㪈 㪇 㪇 㪉 㪇 㪇 㪊 㪇 㪇 㪋 㪇 㪇
㪇 㪌 㪈 㪇 㪈 㪌 㪉 㪇
ᬀᨋᓟ䈱⚻ㆊᐕᢙ
㪾㪛㪤㪆㫄㪉
࿑㧝 ’↪ᨋ㊁ ࿑㧞 ᬀᨋᓟߩ⚻ㆊᐕᢙߣน㘩ㇱሽ㊂ߣߩ㑐ଥ Y = 32.6 + 14.9X (Y=gDM/m2; X=⚻ㆊᐕᢙ; r=0.9, p<0.001)
น㘩ᬀ‛ሽ㊂ߩട㊀ᐔဋ୯:133gDM/m2
㧞㧚⎇ⓥ㆐ߩ⁁
㧝㧕ᨋߩਅጀߦߪᄙ㊂ߩน㘩ᬀ‛߇5߆ࠄ10ߩ’ᦼ㑆ਛ⼾ንߦሽߔࠆߎߣޔ߹
ߚޔᐕ㑆ࠍㅢߓߚ’ోߩ CP ㊂ߪ 11.9%ߢࠆߎߣ߆ࠄޔᨋߪ‐ߩ㙄႐ߣߒߡߩ
㊂⊛⾰⊛ᔅⷐ᧦ઙࠍ߶߷ḩߚߔߣ⠨߃ࠄࠇࠆ(᧖ጊࠄ(2004))ޕ
㧞㧕‐ฦߪޔᐔဋ13㨪14ha/ᣣࠍ↪ߔࠆ(࿑㧟)ޕߘߩ㕙Ⓧߪޔਅጀᬀ‛ߩCP㊂߇ ᦨ߽㜞ᤨᦼߢࠆ56ߦᐢߊޔCP㊂߅ࠃ߮☻❫⛽(CF)㊂߇ᦨ߽ૐᤨᦼߢࠆ10
ߦ⁜ߊߥࠆޕᦨ߽↪㕙Ⓧ߇⁜ߊߥࠆ10ߦߪޔ‐ߪዊ⟲ߦಽ߆ࠇޔᢔ㐿ߔࠆߎߣ(࿑㧠) ߆ࠄ⋙ⷞߪ࿎㔍ߦߥࠅ(⮮(2005)ޔᳯ⮮(2005))ޔߘߩቄ▵એ೨ߢߩ⚳’߇ᔅⷐߢࠆޕ
ੱᎿᨋ ᐢ⪲᮸ᨋ
ⶄጀᨋ
㧟㧕ߎߩࠃ߁ߥᨋ㑆’ߪޔფേ‛⋧߿㊁↢േ‛⋧ߩᄙ᭽ᕈߦᓇ㗀ߪߥߊޔࡃࡦࠬߩ ขࠇߚᜬ⛯⊛↢ᘒ♽ࠨࡆࠬ߇ឭଏߢ߈ࠆࠪࠬ࠹ࡓߢࠆߎߣ߇⏕ߐࠇߚޕ
㪇 㪌 㪈 㪇 㪈 㪌 㪉 㪇 㪉 㪌
㪌 㪍 㪎 㪏 㪐 㪈 㪇
㪿㪸
㪉 㪇 㪇 㪇 㩿㪉 㪀 㪉 㪇 㪇 㪈 㩿㪌 㪀
㪇 㪉 㪇 㪇 㪋 㪇 㪇 㪍 㪇 㪇 㪏 㪇 㪇
㪌 㪏 㪈 㪇
㪿㪸
㪈㪐㪐㪎 㪈㪐㪐㪏
࿑㧟 㧝ᣣⴕേ㕙Ⓧߩ㑆ᄌേ ࿑㧠 ో㗡ⴕേ㕙Ⓧߩቄ▵㑆Ꮕ
㧟㧚ᛛⴚታ↪ൻߩ⁁
ࠬࠡੱᎿᨋ50ᐕબᦼᣉⴕߦࠃࠆᜬ⛯⊛ᨋ㑆’ࠪࠬ࠹ࡓࠍឭ᩺ߒߚޕࡉ࠽ࡒ࠭࠽ࠍਛ ᔃߣߒߚᐢ⪲᮸ᨋߦ࿐߹ࠇߚ1ᨋ⃰10haࠍනߣߒߡᲤᐕᬀᨋࠍታᣉߒޔ50ᐕᓟߦⓠߔ ࠆࠪࠬ࠹ࡓߢࠆޕ500ha ߩੱᎿᨋߦ 208DM ࠻ࡦޔߘࠇࠍขࠅᏎߊ 300ha ߩᐢ⪲᮸ᨋߦ
945DM࠻ࡦߩน㘩ᬀ‛ሽ㊂߇ሽߒޔߘߎߢߪ30㗡ߩ❥ᱺ‐⟲ࠍᜬ⛯⊛ߦ170ᣣ㑆’
ߢ߈ࠆޕᔅⷐੇ‛ⷐ᳞㊂ߪ 45DM࠻ࡦߢࠆ߇ޔᜬ⛯⊛↪ࠍ⠨߃ߚ႐วߪޔ↪₸ࠍ 4%
⒟ᐲߣߔࠆޕ
㧠㧚ᓟߩᣇะ⺖㗴
ᧄᛛⴚߪޔ߈ࠊߡ᧪ᕈߩ㜞ᛛⴚߢࠆޕᓥ᧪ߩ㘩ᢱଏ⛎ࠨࡆࠬߩߺߦ․ൻߒߚ↢
ᘒ♽ߩ↪ߢߪޔࡒ࠾ࠕࡓࠍਸ਼ࠅಾࠇߥߎߣ߇ޔ࿖ㅪⅣႺ⸘↹ߩോዪ߆ࠄ2005ᐕߦ
ߐࠇߚޟ↢ᘒ♽ࠨࡆࠬߣੱ㘃ߩ᧪ޠߢࠄ߆ߣߥߞߚޕଏ⛎ࠨࡆࠬߦട߃ޔၮ⋚( ფ╬)ޔ⺞ᢛ(᳇ᵩ᳓╬)ޔᢥൻߩฦࠨࡆࠬࠍࡃࡦࠬࠃߊឭଏߢ߈ࠆ’ࠪࠬ࠹ࡓ᭴▽
ߩ╙㧝ᱠߣߒߡޔᧄᛛⴚࠍ⹏ଔߔࠆᔅⷐ߇ࠆޕ 㧡㧚ᆔ⸤⺖㗴ߦ㑐ㅪߒߚਥⷐߥႎ๔╬
㧝㧕⮮ⴐ㧦ᅏጊ࿖ᨋߩή’ᩋ⡺↪‐’↪ࠪࠬ࠹ࡓߩឭ᩺ޔ㑐᧲⇓↥ቇળႎ 55(2):115-119. 2005.
㧞㧕᧖ጊߺߤࠅ⮮ⴐᳯஜญༀ㓉⩲ේᄦ㧦ᅏ⠀ጊ♽’↪ᨋ㊁ߦ߅ߌ ࠆน㘩ㇱሽ㊂߅ࠃ߮ᩕ㙃ଔߩಽᏓޔᣣᧄ⨲ቇળ 50(3):294-299. 2004.
㧟㧕ᳯஜ⮮ⴐ㧦ή’ᩋᨋ㑆’ਅߢߩኅ⇓࠙ࠪߩណ㙄ⴕേߣ▤ℂޔູ㘃⑼ቇ 45(1):105-110. 2005.
a b b ab b b
