ポリエチレン1炭化水素粒子に吸着したクロロフィ
ルとアミドの会合.蛍光に及ぼす温度の影響
著者
KUSUMOTO Yoshifumi, SENTHILATHIPAN Velu, SEELY
Gilbert R.
journal or
publication title
鹿児島大学理科報告
volume
43
page range
9-24
year
1986
別言語のタイトル
Association of Chlorophyll with Amides on
Plasticized Polyethylene Particles. Effect of
Temperature on Fluorescence
Ｉｎａｎｕｍｂｅｒｏｉ､instances，theHuorescenceofchlorophylladsorbedwilhotheramphiphilestoswollen polyethyleneparticlesisreducedinilltensltyollcoolingbelowambienttemperatul．e，ｂｕｔｉｓｒｅｓｔｏｌ･edby mrlhercoolingtoliquidnitl･ogentempcrature，TheHuorescencevariationisascrjbedlochangesinthe stateofassociationofchlol-ophyllwithtempel･ature，ａｌｌｄｔｏｃｈａｎｇｅｓｉｎｔｈｅｅ価cienciesofellergyllrappmg andofHuorescenceofaggregatedchlorophylllbl･ｍｓ・Thechangcsobservedoncoolingaremgeneral reversedonrewarmlng・TheHuorescencepropeI･tieｓｏ｢chlorophylladsorbedwithN-(3-pyridyl)myristamide toparticlesswollenwithundecane，tetl･adecane，ａｎｄｓｑｕａｌｅｎｅａｒｅｃｏｍｐａｒｅｄ･Inthecaseofsqualene， wherelittlechangcinlhestateofchlorophyllassociationisexpected，１henuorescencespectrumchanges glraduallyIromthehigh-temperaturｅｔｏｔｈｅｌｏｗ−ｔｅmperaturefolmastrappillgbccomｅｓｍｏｒｅｅ伍cientand mternalconversionless、Withtetradecane-swollenparticles，nuorescenceisabruptlyreducedbelowthe meltingpoint（6.C）ofthediluent，probablybecauseofthelbrmationofweaklyinteractmgpalrsof chlorophyllwhichquenchsingletexcitedstates，Withundccane-swollenarticles，thereductionm Huorescenceoncoolingismoregradualandisprobablydｕｅｔｏｌｌ･appingbyanmcreasmgconcentratlon ofassociatedchlorophyllformswhichHuorescelｅｓｓｅ価cientlythanmonomeI･icchlol･ophyll・Therelssome dependenceonthecomositionoflhepasteinwhichtheparticlesaresuspendedforspectralmeasurement： PasteswhichcontainCarbowax，aweakligaI1dforchlorophyll，favorlhefluorescenceoflesshighly associatedspeciesthanpastescomposedentirelyofcarbohydrales． Sci･Rep‘KagoshimaUniv.，Ｎｏ．４３，ｐｐ、９−２４，Sept.，1994.
Associatio皿OfChlor0phylIwithAmidesonPlasticizedPolyethylene
ParticlesoEimectofTemperatureo皿剛胆orescence
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Ｔ２．フー２２２２１ ｽ/nｍ： B/nｍ： ＦＧＣ ４５．４ ４７．５ 57.1 735 ４４ 730 ３５ 754 ２３ 692 １６ ２９．０ ２２．２ 15.5 678.7 ２０ ８．７ １２．９ 13.4 693.5 １６ T/Ｋ 283 235 ８７ ６．９ ７．０ ６．５ ７６６ ３５ 入/nｍ： B/nｍ： ,1０．０ １０．５ ７．５ ７０６ １９ a）Theposilion（入）andbandwidth（B）ｏｆｅａｃｈｃｏｍｐｏｎｅｎｔａｒｅ ｌｉｓｔｅｄａｔｔｈｅｈighestandlowesttemperaturereported・Posi-tlonsandbandwidthsatintermediatetemperaturesareglven fairlywellbyinterpolation b）Includesammorcomponelltat747､5,ｍ,Ｂ＝１４，，，２３％．ＲＥＬＡＴＩＶＥＦＬＵＯＲＥＳＣＥＮＣＥｌＮＴＥＮＳｌＴＹ 司旨Ｏ民①、○①ロ○①、ロ①○再四○﹃○三○尾○℃云琶一一○旨勺両斎ｐ二四一ｍｐｍ己騨﹃茸○一①切言２．①く①局壁切ヨ昼］四時葛壷①ｇ⑦﹃ 呂名ｇＱａヨ句ＱｏｏＨｏＱ尋︾四国ｇ８国一雪５岩①ｍｏｇ８］昌呂巴ご＆ユロ○︵号貝３ｍｍの揖昌旬＆昌一琶呂 ○○○一一画、、胃ロ①詳宮①局己四ｍ芹①︺ゆめ︵ず①のＰご︺で一①一ｍ○○○一①。二］①、胃①ロ、計ぽい○﹃計彦①α℃い煙ご旦剖いつロヨ 庁声①Ｈ①、○］屋︵一○口吻巴同︺で］琶○○一一①○房く時ケ境四画○国巴、四一①一一戸①四○［ず騨冒。、四芹丘胃、ロ①Ｈ①ロ①局四①ｍ四口Ｑ房巨邑一岸①一旨 ︹○○○旨︵津﹃唇酉巨○吋①ｍ○①ご○①○﹃四。爵毎回○︵、で①○］①の． ］↑ 司狩．い、国巨日①ｍｏｇ８砦⑦只国○﹃○三○﹃○つご一一旦８３８乏琶］Ｐ︲ｚも旨シ８で両一冨言且①ｏｇの ロ騨昌○一①、︾四旨旦呂号９号Ｑ旨○○毒．胃の①く⑦国一一①言一︺①国言忌め． ＲＥＬＡＴｌＶＥＦＬＵＯＲＥＳＣＥＮＣＥＩＮＴＥＮＳｌＴＹ 珂信。桑．司盲○局のの○①冒。⑦の冠①○胃騨○﹃吾のの臼巨①ご煙﹃冨○︸①己冨で煙舜昏言○口四ｍ言句揖．い︾ず匡骨ｍ巨切も③目。①ロ
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乏憂くｍＦｍｚの﹃工言ヨ ＫＯ、宣言冒三穴ご召冨○月ｐく①一二胃ｚ﹃雷偏ン目雪里ｚ・津冒。Ｏ葺き①鼻丙．、用尾 冒司ｏｎ703 １８ ８．６ 7.1 ７．６ ９．５ ７０３ １３ TemperatureEIIbctollAssociatioｎｏ「Chlol･ophyllwithAmides 1５ componentsmcreasesteadily，ａｓｓｈｏｗｎｉｎＦｉｇ２ｆｂｒｔｈｅＦＧＣｓｕｓｐｅｎｓｌon・Theanalysisof thedistributionintocomponents（Tableｌ）showslittlechangefrom295to221K，beyonda gradualdecreaseinthemonomercomponent（676,ｍ)．Allcomponentsaresomewhat red-shinedwithrespecttothoseinPE/tetradecaneandPE/undecalleparticles，whichis pl-obablyamediumeffbctｏｆｔｈｅｍｏｌ･epolarizablesqualene． ＡlthoughthereislittleChangeinthefluorescenceofchlorophyllonPE/tetradecaneparticles abovethemeltingpoint(279Ｋ),thereismarkedquenchingbelowthispoint,notonlyofthe monomercomponent（67111ｍ）ｂｕｔｏｆｔｈｅｏｔｈｅｒｃｏｍｐｏｎｅｎｔｂａｎｄｓａｓｗｅｌＬＴｈｉｓｉｓｔｒｕｅｉｎ ｂｏｔｈＣＧＷａｎｄＦＧＣ；howeveronfurthercooling，ｉｔｉｓｔｈｅ６８８ｎｍｃｏｍｐｏｎｅｎｔｔｈａｔｇａｍｓ ｍｏｓｔｉｎｉｎｔｅｎｓｉｔｙｉｎＣＧＷ，ｗｈｅｒｅａｓｉｎＦＧＣ，ｉｔｉｓｃｏｍｐｏｎｅｎｔｓｉｎｔｈｅ６９５−７０５ｎｍreglon･Ｔｈｅ ｓｐｅｃｔｒａｌｃｈａｎｇｅｓｉｎＣＧＷａｎｄＦＧＣｓuspenslonsareshowninFigs､３ａｎｄ４；analysesmto Table２．DistributionofFluol･escencelntensilyamongComponenls,lｂｒＣｈｌａｗｉｔｈ ３−ＮＰＭＡｏｎＰＥ/Tetl･adecaneParticlesSuspendedinCGW
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ｽ/nｍ： B/nｍ： ６７１．８ １９ Percent ２８．０ ２９．１ 685.7６９８ １ ５ ． ８ １ ９ oftotalHuorescence １２．４１０．８ １２．１１０．９ ７０９ １４ intensity O,９ １‘２ 727 ３８ 7５１ ３１ T/Ｋ 287 278 41.5 43.3 6.4 3.5 入/nｍ： B/nｍ： ８．９ ３．５ １０．６ ８．８ １６．６ 21.7 779 ４６ A/nｍ:"） Ｂ/nｍ： 6７１ ２０ 23.1 13.3 ９．１ ０ ６７４ １９ 687.8 １８ １８．０ ２５．５ ２８．４ 36.0 688.9 １９．５ a）Thesetofcomponentsemployedabovethemeltmgpointoftetradecanecould notbeadaptedtonuorescencebelowthemeltingpolnt，Ｔｈｅ725.5nm component，whichdecreaseswltntemperature，mayincludeavibrational satelliteofthemonomercomponent（671,ｍ)． 713 １９ １．８ ７．３ ７．７ ９．７ ７１３．５ １６ ４９．０ ５３．６ ５２．５ ５３．３ ５０．６ 47.9 735 ４６ 733 ４５ ４６．５ ４４．８ ４５．８ 39.9 739.5 ４２ 272 267 231.5 ８９ 728 ４１ 684.8 １５．５ oftotal ８．４ ９．２ １０．６ １１．７ １０．２ ７．４ ６８６ １５ ｍｍ姉恥取麺加刻麺加卯加恥
戸、 Table３．DistributionofFluorescencelntensityamongComponents、 ｆｂｒＣｈｌａｗｉｔｈ３−ＮＰＭＡｏｎＰＥ/TetradecaneParticlesSuspen‐ ｄｅｄｉｎＦＧＣ 670.3 １９ Percent ２４．７ ２５．１ １４．１ １１．９ ８．５ ９．０ ６７５ １７ 6 9 5 . 7 ７ ０ ５ ７ １ ５ ． ５ １ ５ １ ４ １ ５ ． ５ Huorescenceintensity ５ ． ７ ３ ． ３ ５ ４ ３ ． ２ ７ ． ３ ４ １ ０ ． ９ ８ ． ０ ４ ． ５ １ ． ８ ７ ． ５ ４ ． ８ ２ ． ３ ８ ． ３ ５ ． ６ ３ ． ２ 6 9 6 . 5 ７ ０ ６ 7 1 5 . 5 １ ５ １ ５ １ ５ ． ５ 764 ４４1．０ ０８ 一 一 一 ▲ YoshihumiKusuMoTo･VeluSENTHILATHIPAN，andGilbertR・SEELY １６ T/Ｋ 1２３ ９０ 2 ７ ９ ２ ４ ８ 2 0 1.5 Ｉ ； ９.’ 0.2 １ 画 一 一 一 一 一 口 一 一 画 ' １ ４ ［園 隠 割０６ 0.4 115 ６ ０ lｏｗｅｒ ‐ . ー ３ ． ４ ３ ８ ４ ． ２ ４ ． ６ ９ . ’ ’ 1 . 0 ｜ ￨ ､ ５ ｋK/ｒ Fig.５．Plotsoftotalintegratednuorescenceintellsity(I『,onlogarithmicscale),、orinalized tolatlhehighesttempel･atul･erecorded，agaillstreclpl･ocaltempel・atLlreIbr chlorophyllwith3-NPMAonPE/squalene（C3o)，ＰＥ/ulldecane（Ｃｌ,)、ａｎｄ ＰＥ/tetradecane（Ｃ１４)，ｓｕｓｐｅｎｄｅｄｉｎＦＧＣ（opensymbols）ａｎｄＣＧＷ（solid symbols)．Themellingpomtsoftetl･adecane（279Ｋ）andLmdecane（248Ｋ）are indicated、Theslopesofthedashedlinesconneｃｔｉｎｇｔｈｅｌａｓｔｔｗｏｐｏｉｎｔｓｏｆｅａｃｈ ｓｅｔａｒｅｄｒａｗｎｔｏｓｃａｌｅ．
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