0.1 mmol/L EGTA, 5 mmol/L ATP, 10 mmol/L HEPES, 150 mmol/L K-aspartate, 0.25 mmol/L MgCl2, and 10 mmol/L reduced
glutathione
MaxChelator (http://www.stanford.edu/~cpatton/webmaxcS.htm) Ca2+
internal solution(0.5 mmol/L EGTA, 10 mmol/L HEPES, 120 mmol/L K-aspartate, 5 mmol/L ATP, 1 mmol/L free [Mg2+], 10 mmol/L reduced glutathione, 30 nmol/L free [Ca2+])
** P<0.01 vs Sham baseline ***P<0.001 vs Sham baseline # P<0.05 vs TAC baseline ###P<0.001 vs TAC baseline
Nuclear factor of activated T-cells (NFAT)
CaMBD calmodulin binding domain;