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■ೊ൰ʙʻĜʠ ■ʄɼʡˁɼˋ
■ʂˋˁɼˋʙʽĜʠ˂ɺ˃
mRNAෂɈৌᆎin vitroʅʻʛʯ
ȤɢɍྥʅʻʛʯਗɈʙʽĜʠ˂ ɺ˃ކĪܑাīɬ༯ఒෂ
NEB
Ɉ
RNA
ॸ࢞ᅙᄞć
ဍঢ়ȞˁɼˋʢʛʯɁ
RNA
ॸ࢞ɬʍʵĜʠ
RNAယાɉȳɓɀɈ౺ဘޢɅȤȞɀĂഽହഽᅕɄ੧ၲ࡙༑Ʌ࠲ᅇȱɀȞɘȳăȭɈȭɂɉĂঈ ɈRNAˇĜ˃ʡȦശ੭ȱȹɂȞȠݜತɬཱ܇ȱɀȞɥɈȥɜȱɦɘȵɭăRNAɉۆ๙௶ၡɈ๙ൿ Ʌज़ȩȭɂɈɁȧɄȞယાɁȜɤĂݛߔཱܾɬఌ༲ȱĂۆ๙ાཡএɅ܆ࣗɬᅇȢɀȞɘȳă
ࣷ༃ĂRNAɈ౫সယાɂȱɀɈᄜߠɅ࠲ȳɥބęɈᆅސɉ॔โɅဿɩɤɘȱȹăഽౘɈఛȹɄฝ ယાRNAɞယાʦˋʋĜʟɻˋʈRNAȦĂᅅപȯɦɀȞɄȥȽȹɢȠɄ࡙༑ɬ૩ȾȭɂȦၡਠ ȯɦĂȷɈȹɛ౺ဘߔɅȤȞɀRNAɅශᄆȱȹॸ࢞ȦɍᄆɬୠɛɥɢȠɅɄɤɘȱȹă
small RNAɉĂۆ๙ાཡএɈ๖ଖষ෮ಣɅ୭ᅟɄᄜߠɬݪȹȱɘȳăRNAiɉೀ්ȤɢɍఆဘɁ ɅཡॽȯɦɘȱȹȦĂRNAɅɢɥཡএ౫সɉణ߇౺ဘɁঢ়ཱྀڱɅॽɣɦɘȳăঈ߇౺ဘɅȤȞ ɀɜວᅕɈฝယાRNAɅɢɥ౫সॄᇭȦശ੭ȱɀȞɘȳăణ߇౺ဘɈฝယાRNAɉĂཡ౺Ă࠼ݛĂ ৣɾɼ˃ʑ႕স࡙৭Ăʇ˅ʶʙˋୌఄɅȤȫɥۆ๙ાཡএɈ౫সɄɃɅȤȞɀड़ฤโɄᄜߠɬݪȹ ȱɀȞɘȳăॸ࢞ଞȹȻɉฝယાRNAɬᆀᅙȱɀĂȭɦɘɁݢ༑ɁȜȽȹۆ๙ાɈ࡙༑ސɬ ȢɥɢȠɅɄɤɘȱȹă
NEBɉȭɦɘɁɂວᅕɅ࿚ଋɈᄞɬอࢷȳɥȭɂɅɢȽɀRNAॸ࢞ɬʍʵĜʠȱɀȞȧɘȳă
RNAယાɈਗĂʯ˅ʓʛʏˋʈĂʠˁˋʑʭɿʇʏʿˋĂඍᆋĂސĂഝဍĂʋʬĜĂʇ˅Ĝ
ʣˋʈɅᅙȳɥʜĜ˃ɄɃഽࡎɅɥ౿࿚ɬอࢷȱɘȳăɘȹĂNEBɁɉۜȧറȧఛࡤਊ೧ɞ
ᄞɄɃɈRNAॸ࢞ʜĜ˃ɬާཡȱĂಪɈRNAॸ࢞Ʌ़ɩɥޣᅕɅȤȫȱɘȳă
Small Silencing
RNAs
mRNA
Cap Structure
RNA Polymerase
Ribosome
Silencing Complex
RNAi
AAA
AAA
AAA In vitro RNA Synthesis & Processing
Isolation
Cloning Processing & Analysis
Expression Analysis Reporter Plasmids - T7 Quick High-Yield RNA Synthesis Kit
- ShortCut® RNase III (Short RNAs) - Cap Structure Analogs & Nucleotides - T7 High Yield RNA Synthesis Kit - Vaccinia Capping Enzyme
- Luciferase
- Cellular Imaging & Analysis
- Magnetic mRNA Isolation Kit - polyA Spin™ mRNA Isolation Kit - Oligo d(T)25 Magnetic Beads - Oligo d(T)25 Cellulose Beads
- RNA Ligases - MW Markers - Labeling Enzymes - RNases - PCR, qPCR, RT-PCR - RNase Inhibitors
- Reporters and Activity Assays (Luciferase) - Labeling (SNAP-tag®, CLIP-Tag™, MCP-tag, ACP-tag) - PCR, qPCR and RT-PCR
- NEBNext® Reagents for Library Preparation (mRNA and small RNA)
6
RNA
ॸ࢞ᅙᄞ
= ˂ʋˋʫʢˋʠਊ೧ Avoiding Ribonuclease Contamination 166
RNA
Synthesis
T7 Quick High Yield RNA Synthesis Kit 167 T7 High Yield RNA Synthesis Kit 167
T7 RNA Polymerase 168
SP6 RNA Polymerase 168
E. coli Poly(A) Polymerase 169
Poly(U) Polymerase 169
E. coli RNA Polymerase, (Core and Holo Enzyme) 170
Ribonucleotide Solution Mix 170
Ribonucleotide Solution Set 170
Pyrophosphatase, Inorganic (E. coli, yeast) 170 Thermostable Inorganic Pyrophosphatase 124
Vaccinia Capping System 171
RNA Cap Structure Analogs 171
mRNA Cap 2´-O-Methyltransferase 171
cDNA
Synthesis
cDNA Synthesis Selection Chart 172 ProtoScript II Reverse Transcriptase 172 M-MuLV Reverse Transcriptase 173
AMV Reverse Transcriptase 173
ProtoScript II First Strand cDNA Synthesis Kit 173 ProtoScript First Strand cDNA Synthesis Kit 174 AMV First Strand cDNA Synthesis Kit 174
Primers for cDNA Synthesis 174
RNA Ligases and Modifying Enzymes
Reported Activities for T4 RNA Ligases 175RNA Ligase Selection Chart 175
T4 RNA Ligase 1 176
T4 RNA Ligase 2 176
T4 RNA Ligase 2, truncated 176
T4 RNA Ligase 2, truncated K227Q and KQ 177 Thermostable 5´ AppDNA/RNA Ligase 177
5´ DNA Adenylation Kit 177
HEN1 miRNA Methyltransferase 178 RNA 5´ Pyrophosphohydrolase (RppH) 178
5´ Deadenylase 178
RNase If 179
RNase H 179
RNase HII 179
Antarctic Phosphatase 123
CIP 123
T4 Polynucleotide Kinase 122
ShortCut RNase III 180
XRN-1 180
Exonuclease T 180
RNase
Control
RNase Inhibitor, Murine 181
RNase Inhibitor, Human Placenta 181 Ribonucleoside Vanadyl Complex 181 RNase Contamination Assay Kit 181
RNA Library Preparation for
Next Generation Squencing
NEBNext Ultra RNA Library Prep Kit for Illumina 141 NEBNext Ultra Directional RNA Library Prep Kit
for Illumina 141
NEBNext mRNA Library Prep Master Mix Set for Illumina 141 NEBNext mRNA Library Prep Reagent Set for Illumina 149 NEBNext Multiplex Small RNA Library Prep Set
for Illumina (1-12) 149
NEBNext Small RNA Library Prep Set for Illumina
(Multiplex Compatible) 142
NEBNext mRNA Library Prep Master Mix Set for 454 145 NEBNext Multiplex Small RNA Library Prep Set
for SOLiD (Set 1) 147
NEBNext Small RNA Library Prep Set for SOLiD 147 NEBNext Poly(A) mRNA Magnetic Isolation Module 145 NEBNext Magnesium RNA Fragmentation Module 145 NEBNext RNase III RNA Fragmentation Module 141 NEBNext RNA First Strand Synthesis Module 141 NEBNext mRNA Second Strand Synthesis Module 141 NEBNext Multiplex Oligos (Index Primers Set 1,2) 148
NEBNext Singleplex Oligos 141
DNase I 182
RNA Isolation and Detection
Magnetic mRNA Isolation Kit 183
Oligo d(T)25 Magnetic Beads 183 Oligo d(T)25 Cellulose Beads 183
Streptavidin Magnetic Beads 183
polyA Spin mRNA Isolation Kit 184
p19 siRNA Binding Protein 184
p19 miRNA Detection Kit 184
RNA Markers and Ladders
siRNA Marker 185
dsRNA Ladder 185
microRNA Marker 185
ssRNA Ladder 185
Low Range ssRNA Ladder 185
RNA Loading Dye (2X) 185
Universal miRNA Cloning Linker 185
Avoiding Ribonuclease Contamination
RNAɉĂ2ŌڮɈʪʡ˅ʅʏࡊȦ࢙߇੬ɂȱɀລȩȹɛĂDNAɢɤɜယސȯɦɞȳȞăഽȩɈRNaseɉ2Ō ڮɈʪʡ˅ʅʏࡊɬཱܾହɂȱɀᆀᅙȳɥȭɂɁĂࣸമɼʂˋɈྼᅟ౯ɬޑྤȱɀȞɥăȭɦɣɈ RNaseɉࣸമʅ˄Ĝʠ੬Ʌ൏౯ȦȜɤĂRNase Aʭɹʷ˂Ĝਊ೧ɄɃɈێငɈRNaseɉෳ૪࠰Ɉଛဖ ɞʂĜʠʇ˄ĜʮɬȽɀɜߤ౯ȦฝݚȱɄȞăRNase AळɈਊ೧ɉʪʑʙʐˋરࡊɬਊ೧ߤ౯ɅᅙȞ
ɀȞɥȹɛĪ1īĂʪʑʙʐˋູڽโɄɺ˃ʅ˃ݛ੬ɁȜɥʐʀʙ˃ʬ˅ʃĜʴʥĜʠĪDEPCīɅɢȽ
ɀߤ౯ݛɁȧɥă
RNaseʋˋʗʷʥĜʏʿˋɈঈۚć
RNaseɉঈ߇౺ဘȥɣణ߇౺ဘɘɁĂȳɓɀɈ੧ၲହĂ೨ఉɅശ੭ȳɥăȭɦɣɈਊ೧ɉێཾโɅྥ௵ Ʌߤ౯ȦȞăȷɈȹɛRNaseȦRNAʍˋʯ˃ɅɩȴȥɅʋˋʗʷʥĜʏʿˋȱȹȺȫɁɜRNAɬ༟ ޓȳɥăێཾโɄॸ࢞ଆɁRNaseɈʋˋʗʷʥĜʏʿˋɈঈۚɂȱɀڬݚɈɢȠɄɜɈȦȜɥă
■ ଌঅɅᅙȞɥᅗܕɞᄞ
■ ୈ၃ȥɣɈႉᇮĪRNaseɉऋෂɞĂഽȩɈဘɈჶĂᏖɈෂɅശ੭ȳɥī
■ ఱɈɞྛɂɈಞఌ
ॸ࢞ଆɁɈᅅ႕Ī2, 3īć
New England BiolabsɈRNAଌঅᅙɈ࿚ଋɬ။ȱȹਊ೧ɉĂRNaseʭ˂ĜɈ౼౿࿚ɁȜɥăȱȥȱĂ ଌঅɈݿาɁĂȯɘȰɘɄʋˋʗʷʥĜʏʿˋɈঈۚȥɣRNaseȦɍʋˋʗʷʥĜʏʿˋȳɥݢ༑౯ ȦȜɥăRNaseɈʋˋʗʷʥĜʏʿˋɉĂଌঅɅȤȫɥȞȩȾȥɈێཾโɄڷ๘Ʌ୦ȠȭɂɁ႕Ȫȭ ɂȦɁȧɥă
■ ଌঅෂɉ௵Ʌ൜ɬශᅙȱĂȭɘɛɅଲɤൖȢɥăྛɞལɈჽɈɕȥĂʡɺʦʮĂʅĜʴĜʡĂ
ဘɄɃRNaseȦʋˋʗʷʥĜʏʿˋȱɀȞɥჶɅఌɦȹষɉྼȴଲɤൖȢɥă
■ RNaseʭ˂ĜɈᄞɬᅙȞɥăݢ༑ɄঔɤRNaseʭ˂ĜȦ။ȯɦȹĂȞଘɀɈʯˁʑʙʛʇ౿ ࿚ɞʭɻ˃ʗĜʙʛʯɬᅙȞɥă
■ RNAଌঅಮᅙɈ௲ஒɬಡȫĂȷɈ௲ஒɬʇ˂ĜˋɅ။Ⱦă
■ ʄˁʑ౿࿚ɉ180Ŏڬ௫Ɂౘ૪࠰ஐᆅȳɥȥĂఛɄ0.1%Īv/vīɈDEPCɘȹɉʀʗʦĜ˃Ʌ1 ૪࠰టȱĂယɬଲɤதȧĂʂĜʠʇ˄ĜʮȱɀܸಹɬதࢦȳɥăʂĜʠʇ˄ĜʮɅɢȽɀĂȳɓɀ ɈཱܾDEPCɉယސȯɦĂʗˋʩʇଋɞRNAɂཱܾȱɄȩɄɥă
■ ʵ˂ʃĜʴʥĜʠɜȱȩɉʵ˂ʑʙ˄ˋ೧੮Ī܋അɄɃīɉĂ3%ݿબݛ೧Ʌ10ယ࠰టȱĂ
ܸಹɬதࢦȳɥăᅙɅɉRNaseʭ˂ĜɈɁɢȩಸȽɀݿબݛ೧ɬಸȞᆔȳă
ᄞɈ෮౿Ī2, 3īć
ݚࡥɈڷɅ୦Ƚɀᄞɬ෮౿ȳɥɂRNaseɈʋˋʗʷʥĜʏʿˋɬৌݪโɅ႕ȪȭɂȦɁȧɥă
■ DEPCɬᅙȱȹฆ௵ɈၭɈൣൖɂȱɀĂʷ˂ǭ™ĪMilliporeīɬᅙȳɥȭɂɁĂࠊɁɉɄ ȞɅȱɧਊ೧ཱܾɬယɅᅦȢɥȭɂȦɁȧĂɕɂɭɃɈRNAଌঅɁယɄRNaseʭ˂ĜɈൔɬ ൿɁȧɥă
■ ᅗܕɬDEPCஐᆅȳɥ௲ਗɉĂɘȴᅗܕ1 LɅൎȱɀ1 mlɈDEPCĪSigmaīɬݡȢɀ1૪࠰਼ȶȹষĂ ʂĜʠʇ˄ĜʮȱɀᅆယɄDEPCɬதȩĪȹȺȱĂTrisɈɢȠɄێࢡɺʷˋࡊɉDEPCɂཱܾȳɥȹɛĂ DEPCஐᆅɉɁȧɄȞă഼ɈݛਗဘɁɜʂĜʠʇ˄Ĝʮ૪ɅڤฤɂɄɥɜɈɉDEPCஐᆅɁȧɄȞīă
■ ᄞɞʨʛʭɹĜĪDTTĂʤʇ˄ʂʙʡĂʶˋʄˋܶīɬ෮౿ȳɥ௲ਗɉĂȦɜȞᄞɬ
ʂĜʠʇ˄ĜʮəɈDEPCɜȱȩɉʷ˂QɅᅗȥȱĂ0.22 ̂MɈʭɻ˃ʗĜɅฆȱɀჱࣳȳɥă
˂ʴʤʇ˄ɺĜʔɈ೫ޮ੬ć
RNAɉĂڬݚɅࢫȬɥRNase೫ޮ੬ɬᅙȞɥȭɂɁɜĂRNaseȥɣ။ীȳɥȭɂȦɁȧɥă
■ RNase Inhibitor, MurineĪNEB #M0314īɉʶɾʑɈRNase೫ޮ੬ɁȜɥăȭɦɉʪʠɞʮʗᄻᅰ
Ɉ೫ޮ੬ɂວȲູ౯ɬ૩ȾȦĂબݛ൏౯ȦȩɄɥɢȠɅޜᆯȯɦɀȞɥȹɛɢɤڤฤɁȜɥĪ4īă
೫ޮ੬ɉĂฝ༏ɈDTTɁɜĪ< 1 mMīߤ౯ɬ။ȾȹɛĂDTTɬฝ༏Ʌ။ȻȹȞଌঅĪ˂ɺ˃ʗ ɼʸRT-PCRɄɃīɅไɁȜɥă
■ ʪʠ൙ྉᄻᅰɈ˂ʋˋʫʢˋʠʗˋʩʇଋɁȜɥĂRNase Inhibitor, Human PlacentaĪNEB #M0307ī ɉRNase AĂBĂCູڽโɄ೫ޮ੬ɁȜɥăʶɾʑɈ೫ޮ੬ɈɢȠɅഽȩɈRNAཱܾĪin vitro๖ଖĂ RT-PCRĂˁɼʊĜʏʿˋຑīɅɜᅙȞɥȭɂȦɁȧɥă
■ Ribonucleoside Vanadyl ComplexĪNEB #S1402īɉRNase AळɈೋھൔɺʢ˅ʈ೫ޮ੬ĪK i = 1X10-5 MīɁȜɤĂ౿࿚ɬᅙȳɥɅ༂ஐᆅɅɢɥઆൌवȦྼᅟɁȜɥăȭɈઆൌɉഽȩɈ RNAယᆋၭɅᅙȳɥȭɂȦɁȧɥȦĂEDTAശ੭ݚɁɈᅙɉైயȯɦɄȞăȭɈઆൌɉRNAଌঅ
ɅᅙȞɥഽȩɈɕȥɈਊ೧ɜ೫ޮȳɥĪ5īă
ઞဦॷć
(1) Fersht, A.R. (1977) Enzyme Structure and Mechanism Freeman, Reading, PA, 325-329. (2) Blumberg, D.D. (1987) Methods Enzymol., 152, 20-24.
(3) Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.), (pp. 7.3-7.5). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
(4) Kim, B.M. et al. (1999) Protein Science, 8, 430-434. (5) Berger, S.L. (1987) Methods Enzymol., 152, 227-234.
RN
A研究用試薬
T7 Quick High Yield RNA Synthesis Kit
#E2050S 50 reactions . . . .¥ 44,500 ತცćT7 Quick High Yield RNA Synthesis Kit
ɉᅕęɄɺʯ˂ʉĜʏʿˋɈȹɛɈᆵࣅȞRNA ๖ଖɬȠȹɛɈʅʛʠɁȜɥăʶʑʗĜʷʛ ʇʑᅕɁȜɥȹɛĂബɅཱܾʓʛʠɺʛʯ ɬȠȭɂȦɁȧɥăDNAʞˋʯ˄ĜʠɈதࢦ ɂRNA౼౿ɈȹɛĂDNase IȤɢɍLiClȦ࠸ɘɦ ɀȞɥăȯɣɅʅʻʛʯɺʢ˅ʈĪARCAīɞఋ ೧࿈ૻʤʇ˄ʂʙʡɬଲɤਮɘȵɥȭɂɅɢɤĂ ʅʻʛʯݛRNAɞఋ೧࿈ૻRNAɬਗȳɥȭɂ Ȧݢ༑ɁȜɥă
T7 Quick KitɈູෳć
●ʶʑʗĜʷʛʇʑᅕɁȜɥȹɛĂཱܾʓʛ
ʠɺʛʯȦࠤ၆
●ᆵࣅȞਗཱܾ
●ʞˋʯ˄ĜʠதࢦɂRNA౼౿ɈȹɛɈᄞȦ
മ
●1ཱܾȜȹɤ180 ̂gɈେᆲ
●ʅʻʛʯɺʢ˅ʈɬᅙȞɀ30 - 40 ̂gɈʅʻʛ ʯݛRNAɬ౿
●வᆲɈʞˋʯ˄Ĝʠȥɣৌᆎɢȩ๖ଖ
●य़ɂᅃސɬटɤ၄ȱɀɜȞߤ౯ɬڿ૩
T7 Quick KitɈ໘ᅎć
— Transcription BufferĪ10Xī — T7 RNA Polymerase Mix — NTP Buffer Mix — FLuc Control Template — DNase I
— LiCl Solution
࠲ᇦ౿࿚ć
Vaccinia Capping System
#M2080S 400 units . . . .¥ 25,000
mRNA Cap 2Ō-O -Methyltransferase #M0366S 2,000 units . . . .¥ 10,000
3Ō-O -Me-m7
G(5)ppp(5)G RNA Cap Structure Analog
#S1411S 1 µmol . . . .¥ 34,000 #S1411L 5 µmol . . . .¥ 136,000
E. coli Poly(A) Polymerase
#M0276S 100 units . . . .¥ 12,000 #M0276L 500 units . . . .¥ 48,000
■੍Ȥɢɍෳ੍RNAɈ౿
■ୌఄʤʇ˄ʂʙʡɈଲɤਮə
■࿈ૻʤʇ˄ʂʙʡɈଲɤਮə
■ʅʻʛʯɺʢ˅ʈɬᅙȞȹʅʻʛʯݛ
RNAɈ౿
T7 High Yield RNA Synthesis Kit
#E2040S 50 reactions . . . .¥ 36,500 ತცćT7 High Yield RNA Synthesis KitɉT7 RNA
ʵ˂ʹˁĜʔɬᅙȞȹRNAɈIn vitro๖ଖɈȹɛ Ɉྥ௵Ʌ୨౯ɈȜɥʏʑʞʸɁȜɥăႨʅʛ ʠɬᅙȞɦɊĂ໘င࿈ૻȱȹRNAĂ๖ଖɂວ૪ ɅʅʻʛʯݛȯɦȹRNAɄɃഽᅕɄRNAɈਗ Ȧݢ༑ɁȜɥăȭɈʅʛʠɅɢȽɀਗȯɦȹ RNAɉĂRNA৭ഢĆ࡙༑ސĂ˂ʴʎɼʸɈॸ ࢞ĂRNase။ীɺʛʓɼȤɢɍʧɼʮ˂ʘɼʔĜ ʏʿˋᅙɈʯ˅ĜʮĂɺˋʙʓˋʑRNAɈॸ࢞Ă RNAiଌঅĂʶɼʇ˅ɺ˄ɼސĂʶɼʇ˅ɼˋ ʐɿʇʏʿˋĂIn vitroႩᄟĂRNAˇʇʙˋɄɃ ɬ࠸ɚഽౘɈɺʯ˂ʉĜʏʿˋɅไɁȜɥă
T7 High Yield Kit໘ᅎć
— T7 RNA Polymerase Mix — Reaction Buffer
— ATPĂGTPĂUTPĂCTPĪ100 mMī — FLuc Control Template
The T7 High Yield KitɈູෳć
● NTPsȦখ်Ʌ࠸ɘɦɥȹɛĂ୨Ʉཱܾʓʛ ʠɺʛʯȦݢ༑
● 1ཱܾȜȹɤ180 ̂gɈେᆲ
● ʅʻʛʯɺʢ˅ʈɬᅙȞɀ30 - 40 ̂gɈʅʻʛ ʯݛRNAɬ౿
● வᆲɈʞˋʯ˄Ĝʠȥɣৌᆎɢȩ๖ଖ
● य़ɂᅃސɬटɤ၄ȱɀɜȞߤ౯ɬڿ૩
࠲ᇦ౿࿚ć
DNase I (RNase-Free)
#M0303S 1,000 units . . . .¥ 12,000 #M0303L 5,000 units . . . .¥ 48,000
RNA Loading Dye (2X)
#B0363S 4 x 1 ml . . . .¥ 8,100
■੍ɍෳ੍RNAɈ౿
■ୌఄʤʇ˄ʂʙʡɈଲɤਮə
■࿈ૻʤʇ˄ʂʙʡɈଲɤਮə
■ʅʻʛʯɺʢ˅ʈɬᅙȞȹʅʻʛʯݛ
RNAɈ౿
■ߤ౯Ȥɢɍฝߤ౯ɈRI࿈ૻʯ
˅Ĝʮɬਗ
167
RN
A研究用試薬
6
RNA Polymerase Selection Chart
౿࿚შ ᅙ ᅎᆲ ࡏႊழ༸ݟ߆
T7 RNA Polymerase
(#M0251)
SP6 RNA Polymerase
(#M0207)
RI࿈ૻʯ˅ĜʮɈ౿ 5,000 /
25,000 units
¥ 12,000 / ¥ 48,000
In vitroႩᄟᅙɈRNA౿
RNAɈ৭ഢĂୌఄݿาĂȤɢɍఌ༲ᅙɈॸ࢞ᅙɈRNA౿ 2,000 /
10,000 units
¥ 11,000 / ¥ 40,000
ɺˋʙʓˋʑRNAɅɢɥཡএ౫স
E. coli RNA Polymerase, Core Enzyme
(#M0550)
E. coli RNA Polymerase, Holoenzyme
(#M0551)
๖ଖާ๘Ɉސ
100 units ¥ 27,000
෬ࣳʯ˅ʺĜʗĜȥɣRNAɬਗ
PURExressɬᅙȞȹin vitroႩᄟ 50 units ¥ 19,500
E. coli Poly(A) Polymerase
(#M0276)
ATPɘȹɉʋ˃ʐʓʬˋ 5Ō˂ˋબɅɢɥRNAɈ࿈ૻ
100 / 500 units
¥ 12,000 / ¥ 48,000
ʇ˅ĜʣˋʈɘȹɉɺʭɻʣʞɻĜ౼౿ɈȹɛɈRNAɒɈ polyĪAīʞĜ˃Ɉݡ
ణ߇౺ဘɒɈRNAຩ૪ɈႩᄟৌᆎɈഝࣅ
Poly(U) Polymerase
(#M0337)
UTPɅɢɥRNAɈ࿈ૻ
60 units ¥ 14,500
RNAɒɈpolyĪUīɘȹɉpolyĪAīʞĜ˃Ɉݡ
ణ߇౺ဘ੧ၲɅຩȯɦȹRNAɈڤฤ౯ɂႩᄟɅɖȳ polyĪUīʞĜ˃Ɉ܆ࣗɈॳ
T7 RNA Polymerase
SP6 RNA Polymerase
T7 RNA Polymerase
#M0251S 5,000 units . . .¥ 12,000 #M0251L 25,000 units . . .¥ 48,000 SP6 RNA Polymerase
#M0207S 2,000 units . . .¥ 11,000 #M0207L 10,000 units . . .¥ 44,000
ತცćT7 RNA PolymeraseȤɢɍSP6 RNA PolymeraseɈ๖ଖɈާɉĂȷɦȸɦT7Ȥɢɍ SP6ʭɹĜʐʯ˅ʺĜʗĜɅൎȳɥູڽ౯Ȧ ȞăȭɈȹɛĂT7ɘȹɉSP6ʯ˅ʺĜʗĜȥɣ ʶ˃ʙʇ˅ĜʣˋʈʍɼʠɘɁ๖ଖɬಞโɅ ᄾຩɁȧɥɢȠɄʇ˅ĜʣˋʈʱʇʗĜȦާཡ ȯɦȹăȭɈɢȠɄʱʇʗĜɬᅙȳɥȭɂɅ ɢ ɤĂ ʇ ˅ Ĝ ʣ ˋ ʈ ȱ ȹDNA༯ ᇙ ȥ ɣ ູ ฤ Ɉ RNA๖ଖદဘɈin vitroਗȦݢ༑ɂɄɥăਗ ȯɦȹRNAɉĂmRNAɂȱɀ౺ဘߔโɅߤ౯Ȧ ȜɤĂ౷ߊɅʑʯˁɼʏˋʈȯɦɥăɘȹĂʇ ˅ĜʣˋʈȱȹDNAɈၫɂࢍȧɅਗ ȯɦȹɺˋʙʓˋʑRNAɉĂin vivoɁmRNAɈ ႩᄟɬູڽโɅʮ˅ʛʇȳɥȭɂȦඪɣɦɀȞ ɥăȯɣɅĂRNAćDNAʧɼʮ˂ʛʡɉRNAć RNAɜȱȩɉDNAćDNAʧɼʮ˂ʛʡɂɓɀ ڤฤ౯ȦȞȹɛĂ߇બʧɼʮ˂ʘɼʔĜʏʿˋ ཱܾɅȤȫɥॳ˄ʱ˃ɜ௫ȳɥă
ᄻᅰćSalmonella typhimurium LT2ZᄻᅰɈSP6 RNA Polymeraseɬʇ˅Ĝʣˋʈȱȹۆ๙ાɬ ᄵȳɥ෬ࣳĂɘȹɉᄾຩݢ༑Ʉlac UV5ʯ˅ ʺĜʗĜɈ౫সݚɅȜɥT7ۆ๙ાɬ࠸ɚʯˁʑ ʷʡpAR1219ɬᄵȳɥ෬ࣳBL21ă
ཱܾ௸ॣć1X RNAPol Reaction BufferĮ40 mM
Tris-HClĪpH 7.9īĂ6 mM MgCl2Ă2 mM spermidine, 10 mM dithiothreitolįă ȷ ɦ ȸ ɦ 0.5 mM ȴ Ⱦ ɈATPĂUTPĂGTPĂCTPĪ ် ᅙڷīɂไಜɄʯ˅ʺĜʗĜɬᄵȳɥDNAʞˋ ʯ˄Ĝʠɬ๒ݡă37ŎĪT7 RNA Polymeraseī ɘ ȹ ɉ40ŎĪSP6 RNA Polymeraseī Ɂ ɼ ˋ ʅʽʱĜʏʿˋă
࿚ଋࠣᆅćRNAʵ˂ʹˁĜʔĂDNaseȤɢɍ
RNaseɈʋˋʗʷʥĜʏʿˋȦॳȯɦɄȞȭ ɂɬߊă
ʾʣʛʠฤࡹć1 unitɉĂ37ŎĪT7 RNA Polymeraaseī
ɘȹɉ40ŎĪSP6 RNA PolymeraseīĂ1૪࠰ɁĂ 1 nmol ATPɬબ౯ᅗဘɅଲɤਮɚȭɂȦɁȧ ɥਊ೧ᆲɂȳɥăʾʣʛʠɺʛʓɼ௸ॣɉwww. nebj.jpɬઞă
༏ćSP6 RNA Polymeraseć20,000 units/mlă T7 RNA Polymeraseć50,000 units/mlă
ઞ৽ဦॷćwww.nebj.jpɬઞ
■RI࿈ૻȱȹRNAʯ˅ĜʮɈ෮౿
■in vitroႩᄟɅᅙȞɥRNAɈ౿
■RNAɈ৭ഢĂୌఄݿาĂȤɢɍఌ༲ ᅙɈॸ࢞ᅙɈRNA౿
■ɺˋʙʓˋʑRNAɅɢɥཡএ౫স
RN
A研究用試薬
E. coli
Poly
Ī
A
ī
Polymerase
#M0276S 100 units . . .¥ 12,000 #M0276L 500 units . . .¥ 48,000
ತცćE. coli PolyĪAīPolymeraseɉĂʞˋʯ ˄ĜʠྥگശโɅRNAɈ3ŌჂɒAMPɬݡȳ ɥਊ೧ɁȜɥă
ᄻ ᅰć ෬ ࣳ ȥ ɣ ʇ ˅ Ĝ ʣ ˋ ʈ ȱ ȹPolyĪAī Polymeraseۆ๙ાɬᄵȳɥ෬ࣳă
ཱ ܾ ௸ ॣć1X PolyĪAīPolymerase Reaction
BufferĮ50 mM Tris-HClĪpH 7.9@25ŎīĂ250 mM NaClĂ10 mM MgCl2įĂ1 mM DTTĂ1 mM ATPɬ๒ݡă37ŎɁɼˋʅʽʱĜʏʿˋă ༂Ʌɢɥߤ౯ݛć65ŎĂ20ယ࠰
മᄞć
10X PolyĪAīPolymerase Reaction Buffer 10 mM ATP
࿚ଋࠣᆅćDNaseĂRNaseĂʳʑʭɹʗĜʔɈ
ʋˋʗʷʥĜʏʿˋȦॳȯɦɄȞȭɂɬߊă
ʾ ʣ ʛ ʠ ฤ ࡹć1 unitɉĂ37ŎĂ10ယ ࠰ ɁĂ1
nmolɈAMPɬRNAɅଲɤਮɚȭɂȦɁȧɥਊ೧ ᆲɂȳɥăʾʣʛʠɺʛʓɼ௸ॣɉwww.nebj.jp ɬઞă
༏ć5,000 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
࠲ᇦ౿࿚ć
Adenosine-5Ō Triphosphate (ATP)
#P0756S 1 ml . . . ¥ 5,000
#P0756L 5 ml . . . ¥ 20,000
RNase Inhibitor, Murine
#M0314S 3,000 units . . . ¥ 12,000 #M0314L 15,000 units . . . ¥ 48,000
■ʋ˃ʐʓʬˋ5Ōજ˂ˋબɘȹɉATPɅ ɢɥRNAɈˁʱ˂ˋʈ
■RNAɬPolyĪAīʞĜ˃ݛȱɀʇ˅Ĝʣˋ ʈɘȹɉɺʭɻʣʞɻĜ౼౿
■ణ߇౺ဘ੧ၲɅຩȯɦȹRNAɈႩᄟ ണమ
Poly
Ī
U
ī
Polymerase
#M0337S 60 units . . .¥ 14,500 ತცćPolyĪUīPolymeraseɉĂʞˋʯ˄Ĝʠ
ྥگശโɅRNAɈ3ŌჂɒUTPᄻᅰɈUMPɘȹ ɉATPᄻᅰɈAMPɬݡȳɥਊ೧ɁȜɥă
ᄻᅰćSchizosaccharomyces pombeɈCid1ȥ ɣʇ˅ĜʣˋʈȱȹPolyĪUīPolymeraseۆ๙ ાɬᄵȳɥ෬ࣳă
ཱܾ௸ॣć1X NEBuffer 2Į50 mM NaClĂ10
mM Tris-HClĂ10 mM MgCl2Ă1 mM DTTĪpH 7.9@25Ŏīįă1 mM UTPĪႨ౿࿚Ʌɉ࠸ɘɦɀ ȞɄȞīɬ๒ݡă37ŎɁɼˋʅʽʱĜʏʿˋă ༂Ʌɢɥߤ౯ݛć65ŎĂ20ယ࠰
࿚ଋࠣᆅćDNaseĂRNaseȤɢɍʳʑʭɹʗĜ
ʔȦॳȯɦɄȞȭɂɬߊă
ʾʣʛʠฤࡹć1 unitɉĂ37ŎĂ10ယ࠰ɁĂཱ
ܾᅎᆲ50 ̂lෂɈ1 nmolɈUMPɬRNAɅଲɤਮ ɚȭɂȦɁȧɥਊ೧ᆲɂȳɥăʾʣʛʠɺʛʓ ɼ௸ॣɉwww.nebj.jpɬઞă
༏ć2,000 units/ml
။ ശ ௸ ॣć10 mM Tris-HClĪpH 7.5īĂ100
mM NaClĂ0.1 mM EDTAĂ1.0 mM DTTĂ Ȥ ɢɍ50%ʈ˂ʓ˅Ĝ˃ă
ʹ ʺćPolyĪUīPolymeraseɉĂNEBuffer 2ෂ Ʌ Ȥ Ȟ ɀĂUTPɘ ȹ ɉATPᄻ ᅰ ɈUMPɘ ȹ ɉ AMPɬRNAɅଲɤਮɚăPolyĪUīʞĜ˃Ɉෳ ȯɉUTPȤɢɍʯˁɼʶĜɈ༏ɅگശȱĂʯ ˁɼʶĜ༏ȦฝȞĪ< 100 pmolī௲ਗɅȞ ʯ˅ʓʛʏʫʞɻĜɬȳă
ઞ৽ဦॷćwww.nebj.jpɬઞ
࠲ᇦ౿࿚ć
RNase Inhibitor, Murine
#M0314S 3,000 units . . . ¥ 12,000 #M0314L 15,000 units . . . ¥ 48,000
Ribonucleotide Solution Set
#N0450S 10 µmol of each . . . ¥ 11,000 #N0450L 50 µmol of each . . . ¥ 44,000
■UTPɅɢɥRNAɈˁʱ˂ˋʈ
■RNAɬPolyĪUī ʞ Ĝ ˃ ݛ ȱ ɀ ʇ ˅ Ĝ ʣˋʈ
■ణ߇౺ဘ੧ၲɅຩȯɦȹRNAɈڤฤ ౯ȤɢɍႩᄟɅൎȳɥPolyĪUīʞĜ˃ ݛȦɖȳ܆ࣗɈॸ࢞
■2ŌO-ʹ ʙ ˃ ݛ ȯ ɦ ȹ3ŌჂ ɒ ɈPoly ĪAīʞĜ˂ˋʈ
169
RN
A研究用試薬
6
E. coli
Polymerase, Core Enzyme
E. coli
Polymerase, Haloenzyme
E. coli
RNA Polymerase, Core Enzyme #M0550S 100 units . . . .¥ 27,000E. coli
RNA Polymerase, Holoenzyme #M0551S 50 units . . . .¥ 19,500ತცćE. coli Polymerase Core Enzymeɉ˷Ă˷Ă
˸ŌĂ˸Ă̎Ɉ5ȾɈʍʮʾʣʛʠȥɣ৭ȯɦ
ɀȞɥăႨਊ೧Ʌɉʏʈʶۚાɉ࠸ɘɦɀȞɄ ȞȹɛĂʨʇʞ˂ɺȤɢɍʭɹĜʐDNAɈʯ˅ ʺĜʗĜȥɣູฤɈ๖ଖɬާȱɄȞăྥູڽ โɄ๖ଖާ༯ᇙȥɣRNAɬ๖ଖȳɥ౯༑ɉᄵ ȱɀȞɥăʏʈʶۚાɬ๒ݡȳɥȭɂɅɢɤĂ ູ ฤ Ɉ ʨ ʇ ʞ ˂ ɺ Ȥ ɢ ɍ ʭ ɹ Ĝ ʐDNAɈ ʯ ˅ ʺĜʗĜȥɣRNAਗɬާȯȵɥȭɂȦɁȧ ɥăCore EnzymeɈယાᆲɉᄝ400 kDaɁȜɥă
E. coli Polymerase Haloenzymeɉ ʏ ʈ ʶ ۚ ા 70ɬᄵȳɥăHaloenzymeɉʏʈʶ70Ʌູڽโ Ʉʨʇʞ˂ɺȤɢɍʭɹĜʐʯ˅ʺĜʗĜȥɣ RNAਗɬާȳɥȭɂȦɁȧɥă
ᄻᅰćE. coli Polymerase Core Enzymeɉ෬ ࣳBL21ȥɣඍᆋăʏʈʶۚાɉʏʈʶۚા70ɬ ʇ˅Ĝʣˋʈȱȹۆ๙ાɬᄵȳɥ෬ࣳȥɣ౼౿ă
ཱܾ௸ॣć40 mM Tris-HClĂpH 7.5Ă150 mM
KClĂ10 mM MgCl2Ă0.01% Triton-X-100Ă 1 mM DTTĂ0.5 mMɈ߂ହrNTPɂDNAʞˋʯ ˄Ĝʠă37ŎɁɼˋʅʽʱĜʏʿˋă
࿚ଋࠣᆅćDNAʀˋʡʤʇ˄ɺĜʔĂʀʅʕʤ
ʇ˄ɺĜʔĂȤɢɍRNaseߤ౯ȦॳȯɦɄȞ ȭɂɬߊă
ʾʣʛʠฤࡹć1ʾʣʛʠɉĂ37ŎĂ10ယ࠰ɁĂ
1 nmolɈNTPɬRNAɅଲɤਮɚȭɂȦɁȧɥਊ ೧ᆲɂȳɥăʾʣʛʠɺʛʓɼ௸ॣɉwww.nebj. jpɬઞă
༏ć1,000 units/ml ■T7ྥگശ౯Ɉ๖ଖާ๘Ɉॸ࢞
■PURExpressĪ#E6800ī ɬ ᅙ Ȟ ȹin
vitroႩᄟ
Ribonucleotides
Ribonucleotide Solution Mix #N0466S 10 µmol of each . . .¥ 12,000 #N0466L 50 µmol of each . . .¥ 48,000 Ribonucleotide Solution Set #N0450S 10 µmol of each . . .¥ 11,000 #N0450L 50 µmol of each . . .¥ 44,000
ತცć
Ribonucleotide Solution Mixć
˂ʴʤʇ˄ʂʙʡ3˂ˋબĪrATPĂrCTPĂrGTPĂ rUTPīɬ࠸ɚຑʺ˃ɈʨʛʭɹĜᅗܕă߂ʤʇ ˄ʂʙʡɬ25 mMɈ༏ɁอࢷĪrNTPɈ༏ ɉ100 mMīă
Ribonucleotide Solution Setć
ȭɈʓʛʠɉATPĂGTPĂCTPĂUTPȦȷɦȸ ɦ်ęɈʙʽĜʮɅ࠸ɘɦĂpH 7.5Ʌ෮ȯɦ ȹʢʠ˂ɾʸܶɂȱɀอࢷă߂˂ʴʤʇ˄ʂʙ ʡɉ100 mMɁอࢷă
࿚ଋࠣᆅć˂ʴʤʇ˄ʂʙʡɅɉRNaseȦ࠸ɘ
ɦɀȞɄȞȭɂɬߊăɉ෮౿ᅙin vitro๖ ଖཱܾɅɢɤॳă
ʹʺćෳࡖ࠰Ɉ။ശɁߤ౯ɬ။૩ȯȵɥȹɛɅ
ɉĂவᆲɅယȫɀ–80ŎɁ။ശă
ˁɼʓˋʑ/ູࢮ/ჲಗૢਐć379 - 382ʲĜʐɬઞ
Pyrophosphatases
Pyrophosphatase, Inorganic (
E. coli
) #M0361S 10 units . . . .¥ 11,000 #M0361L 50 units . . . .¥ 44,000 Pyrophosphatase, Inorganic (yeast) #M2403S 10 units . . . .¥ 11,000 #M2403L 50 units . . .¥ 44,000ತ ცćInorganic pyrophosphataseĪPPaseī ɉჟ࡙ʬ˅˂ˋબɈݡယސɬఌ༲ȱɀʂ˃ʠ ˂ˋબɬ౺ȳɥă
P207 -4
+ H20 Ũ 2HP04 -2
ᄻᅰćPyrophosphatase, InorganicĪE. coliīɉ ෬ࣳᄻᅰɈჟ࡙ʬ˅ʳʑʭɹʗĜʔۆ๙ાɈ ʇ˅Ĝˋȥɣ෮౿ă
Pyrophosphatase, InorganicĪyeastīɉ
Saccharomyces cerevisiaeᄻ ᅰppaۆ ๙ ા ɂ
Mycobacterium xenopiᄻ ᅰGyrAɼ ˋ ʞ ɼ ˋ ۆ ๙ાɈᅃਗۆ๙ાɬᄵȳɥ෬ࣳăNEBɈอ़ ޏଜɁȜɥBiohelix CorporationȦާཡă
࿚ଋࠣᆅćE. coli Pyrophosphatase, Inorganic
ɉ˄ʱ˃Ɉ࡙༑ɂ౼౿ɬȹȳȭɂɬʋˋ ʠ˅Ĝ˃ɺʛʓɼɅɢɤߊă
ʾʣʛʠฤࡹć1ʾʣʛʠɉĂ࿈ஈཱܾ௸ॣݚ
Ī20 mM Tris-HClĂpH 7.5Ă2 mM MgCl2Ă2 mM PPiɬ࠸ɚʨʛʭɹĜෂĂ25ŎĂ10ယ࠰īɁĂ ჟ࡙ʬ˅˂ˋબȥɣႴယ1 ̂molɈ˂ˋબɬ౺ ȳɥȹɛɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦɥă
༏ć100 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
■๖ଖཱܾɅȤȫɥRNAେᆲɬ௫
■DNAထ౿ɬണమ
Thermostable Inorganic Pyrophosphatase
#M0296S 250 units . . . .¥ 12,000#M0296L 1,250 units . . . .¥ 48,000 ੧ɉ124ʲĜʐɬઞ
RN
A研究用試薬
Vaccinia Capping System
#M2080S 400 units . . . .¥ 25,000 ತცćVaccinia Capping SystemɉĂVaccinia
ɾɼ˃ʑɈʅʻʛʯݛਊ೧ɬᆀᅙȱȹʏʑʞʸ ɁĂRNAɈ5ŌჂɬ7-methylguanylateɁʅʻʛ ʯĪCap 0īȳɥȹɛɅྼᅟɄਊ೧ɂᄞȦ࠸ɘ ɦɥăణ߇౺ဘɁɉȭɈჂʅʻʛʯɉmRNA ɈڤฤݛĂᄪഗĂȤɢɍႩᄟɅ࠲ᅇȱɀȞɥă ʅʻʛʯݛȱȹRNAɈਊ೧โɄ౿ɉin vitroႩ ᄟĂʠˁˋʑʭɿʇʏʿˋĂʶɼʇ˅ɼˋʐɿ ʇʏʿˋɅᅙȞɥRNAɈڤฤ౯ɂႩᄟৌᆎɬ ௫ȯȵɥࠤ၆ɄၫၭɁȜɥă഼ɅɜĂ࿈ૻȱȹ GTPɬȭɈཱܾृɅݡȢɥȭɂɁRNAɈ5ŌჂ Ɉજ˂ˋબɬࠤඍɅ࿈ૻɁȧɥă
ȭɈਊ೧ɉ2ȾɈʍʮʾʣʛʠĪD1ȤɢɍD12ī
ȥɣɄɤĂ3ȾɈਊ೧ߤ౯ɬ૩ȾĪD1ʍʮʾʣʛ
ʠɅɢɥRNAʠ˂ʳʑʭɹʗĜʔȤɢɍʈɺʣ ˂˃ʠˁˋʑʭɿˁĜʔĂD12ʍʮʾʣʛʠɅ
ɢɥʈɺʣˋʹʙ˃ʠˁˋʑʭɿˁĜʔīăȭɦ
ɣɉȞȴɦɜࠊɄCap 0৭ഢɁȜɥm7 Gppp Ī5ŌīNɬݡȳɥɈɅྼᅟɁȜɥăin vitro๖ଖ દဘɉĂʅʻʛʯݛਊ೧ĂཱܾʨʛʭɹĜĂGTP Ȥɢɍʹʙ˃ࡊɈʡʢĜɁȜɥSAMɈശ੭ݚɁĂ 1૪࠰ڬ໘ɅʅʻʛʯݛɁȧɥăȭɈʅʻʛʯɈ ݡɉৌᆎȦᆯȩĂȳɓɀɈʅʻʛʯ৭ഢɉ౷ȱ ȞၫɅݡȯɦĂ୦ᅰɈɢȠɅʅʻʛʯɺʢ˅ ʈȦࢍၫɅݡȯɦɥȭɂɉɄȞă
ᄻᅰćVacciniaĪWRīʅʻʛʯݛਊ೧ۆ๙ાɬ ૩Ⱦ෬ࣳă
ཱܾ௸ॣć1X Capping Reaction BufferĮ50 mM
Tris-HClĪpH 8.0Ă25ŎīĂ5 mM KClĂ1 mM DTTĂ1 mM MgCl2įĂ37ŎɁɼˋʅʽʱĜʏʿˋă
Vaccinia Capping SystemɈ໘ᅎć
Vaccinia Capping Enzyme Capping BufferĪ10Xī GTP SolutionĪ10 mMī SAM SolutionĪ32 mMī
࿚ଋࠣᆅć1Ⴈ੍Ă2Ⴈ੍DNAɈʀʅʕʤʇ˄
ɺĜʔĂʀˋʡʤʇ˄ɺĜʔĂRNaseɈʋˋʗ ʷʥĜʏʿˋȦჟȞȭɂɬߊă
ʾʣʛʠฤࡹć1 unitɉĂ37ŎĂ1૪࠰Ɂ10 pmol
ɈĪ˷-32PīGTPɬ80ʤʇ˄ʂʙʡɈ๖ଖદဘɅ ଲɤਮɚɈɅྼᅟɄਊ೧ᆲɂฤࡹȯɦɥă
༏ć10,000 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
࠲ᇦ౿࿚ć
RNase Inhibitor, Murine
#M0314S 3,000 units . . . ¥ 12,000 #M0314L 15,000 units . . . ¥ 48,000
■in vivo Ȥɢɍ in vitroႩᄟɈmRNA Ɉʅʻʛʯݛ
■mRNAɈ5ŌჂɈ࿈ૻ
■1૪࠰ڬ໘ɁʅʻʛʯݛཱܾȦࠊᆡ
RNA Cap Structure Analogs
m7G(5Ō)ppp(5Ō)G
#S1404S 1 µmol . . . . .¥ 34,000 #S1404L 5 µmol . . . .¥ 136,000 m7G(5Ō)ppp(5Ō)A
#S1405S 1 µmol . . . . .¥ 34,000 #S1405L 5 µmol . . . .¥ 136,000 G(5Ō)ppp(5Ō)A
#S1406S 1 µmol . . . . .¥ 22,500 #S1406L 5 µmol . . . . .¥ 90,000 G(5Ō)ppp(5Ō)G
#S1407S 1 µmol . . . . .¥ 21,000 #S1407L 5 µmol . . . . .¥ 84,000 3Ō-O-Me-m7G(5Ō)ppp(5Ō)G* #S1411S 1 µmol . . . . .¥ 34,000 #S1411L 5 µmol . . . .¥ 136,000
ತცćmRNAɈ5ŌჂm7Gʅʻʛʯ৭ഢɉĂɕ ɂɭɃɈణ߇౺ဘɅശ੭ȱĂin vitroɁɈႩᄟާ ɬണమȯȵɥăɕɂɭɃɈRNAɁɉĂʅʻʛʯ৭ ഢɉmRNAɈڤฤ౯ɬഝݡȯȵĂʀʅʕʤʇ˄
ɺĜʔɅɢɥယސɬିȫɅȩȩȱĂmRNAႩᄟ
ާထਗൌɈवɬണమȳɥă5ŌჂʅʻʛʯ ৭ഢɬݡȱȹȜɥହɈঈ߇౺ဘmRNAɉĂణ ߇౺ဘɈჟ੧ၲʗˋʩʇଋਗृɁణ߇౺ဘ
mRNAɂວᅕɅৌᆎโɅႩᄟȯɦɥăణ߇౺ဘ
ɈRNAɈێငɁɉĂʑʯˁɼʏˋʈɅʅʻʛʯ ৭ഢɬྼᅟɂȳɥȭɂȦၡਠȯɦɀȞɥă
SP6 RNAʵ˂ʹˁĜʔĂT7 RNAʵ˂ʹˁĜʔĂ T3 RNAʵ ˂ ʹ ˁ Ĝ ʔ ɬ Ƚ ȹ ๖ ଖ ृ ɁĂ7 methyl Gʅ ʻ ʛ ʯ ɺ ʢ ˅ ʈ Ɂ Ȝ ɥm7GĪ5Ōī pppĪ5ŌīGĪNEB #S1404ī ɬ ʯ ˁ ɼ ʶ Ĝ ɂ ȱɀᅙȳɦɊĂʅʻʛʯݛȯɦȹRNAɬᆲ Ʌ౺ȳɥȭɂȦɁȧɥăT7 RNAʵ˂ʹˁĜʔ ɉ Ɉ ܶ ࡊ ɂ ȱ ɀGɬ ೨ ə ਮ ɚă ȷ Ɉ ȹ ɛ NEB #S1405ɞ#S1406Ɂ๖ଖɂວ૪Ʌʅʻʛ ʯݛȯɦȹRNAɉAʅʻʛʯ৭ഢɬ૩ȾăȭɈA ʅʻʛʯ৭ഢɉĂႩᄟȯɦɄȞȦڤฤȱɀȞɥ ȹɛʠˁˋʑʭɿʇʏʿˋɞʶɼʇ˅ɼˋʐɿ ʇʏʿˋɈଌঅɅไȱɀȞɥă
m7GĪ5ŌīpppĪ5ŌīGĪNEB #S1404īĂɘȹ ɉm7
GĪ5ŌīpppĪ5Ōī AĪNEB #S1405ī Ɂ ʯˁɼʷˋʈȯɦȹE.coli RNA ʵ˂ʹˁĜʔɬ ȽɀৌᆎโɅin vitroɁʅʻʛʯݛRNAɬਗ ȳɥၫၭȦContreasɣɅɢȽɀާཡȯɦȹă
ʅʻʛʯɺʢ˅ʈɉ2ȾɈ3Ōʪʡ˅ʅʏࡊɬ૩Ⱦ ȹɛĂȞȴɦɈၫȥɣɁɜRNAɅ೨əਮɘɦ ɥăAnti-reverse cap analogĪARCAīɁȜɥĂ 3ŌO-Methyl-m7GĪ5ŌīpppĪ5ŌīGĪNEB #S1411īɉʹʙ˃ݛɅɢȽɀʪʡ˅ʅʏࡊɈ1 ȾȦʮ˅ʛʇȯɦɀȞɥȹɛĂRNAɅ೨əਮɘ ɦɥɂȧɉĂྼȴm7GȦɈܶࡊɂɄɥăȭ ɈɢȠɅၫ౯ȦȜɥȭɂɁĂin vitroႩᄟȦৌ ᆎโɅɩɦɥă
25 A260 units ≅ 0.7 - 1.1 mgɘȹɉ0.9 - 1.3 µmol
ઞ৽ဦॷćwww.nebj.jpɬઞ
ŖAnti-Reverse Cap AnalogĪARCAīɂȱ ɀɜඪɣɦɥă
mRNA CAP 2
Ō
-
O
-Methyltransferase
#M0366S 2,000 units . . . .¥ 10,000 ತცćmRNA CAP 2Ō-O-Methyltransferaseɉ
RNAɈ5ŌჂȦʅʻʛʯݛȯɦȹێྈɛɈʤ ʇ˄ʂʙʡɈ2Ō-OငڮɅʹʙ˃ࡊɬݡȳɥă ʹ ʙ ˃ ࡊ ࢷ ᅇ ൌ ɂ ȱ ɀS-adenosylmethyonine ĪSAMīɬᅙȞɀĂʅʻʛʯݛRNAĪCap Oīɬ ʹʙ˃ݛȳɥȭɂɅɢɤĂCap 1৭ഢɬ౺ȳɥă
ᄻ ᅰ ćV a c c i n i a m R N A C a p 2Ō-O- Methyltransferaseۆ๙ાɬᄵȳɥ෬ࣳă
ཱܾ௸ॣć1X Capping Buffer,Į50 mM
Tris-HClĂ5 mM KClĂ1 mM DTTĂ1 mM MgCl2ĂĪpH 8.0@25Ŏīįă37ŎɁɼˋʅʽʱĜʏʿˋă
ʾʣʛʠฤࡹć1ʾʣʛʠɉĂ37ŎĂ1૪࠰ɁĂ80
ntɈෳȞʅʻʛʯݛRNA๖ଖဘɬ10 pmolʹʙ ˃ݛȳɥȹɛɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦɥă
༏ć50,000 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
■ RNAɈႩᄟɬണమ
■ ʶɼʇ˅ɼˋʐɿʇʏʿˋȤɢɍ
ʠˁˋʑʭɿʇʏʿˋෂɈmRNA Ɉཡএɬണమ
171
RN
A研究用試薬
6
cDNA Synthesis Selection Chart
cDNAਗ ູ ᅎᆲ ࡏႊݟ߆
ʅʛʠ
ProtoScript® II First Strand cDNA
Synthesis Kit (#E6560S/L)
၆ᆀɄ2ܕʗɼʯ
30/150 rxns ¥ 18,000/
¥ 72,000
ProtoScript II Reverse Transcriptaseɬ࠸ɚ M-MuLVɂߏȱɀ൏༂౯Ȧ௫
RNase Hߤ౯Ȧฝঌ > 10 kbɈcDNAɬਗ
dNTPsĂʂ˂ʌdTʯˁɼʶĜĂˁˋʘʸʯˁɼʶĜʷʛʇʑɬ࠸ɚ
ProtoScript First Strand cDNA Synthesis Kit (#E6300S/L)
ࢤ౿࿚შćProtoScript M-MuLV First Strand cDNA Synthesis Kit
၆ᆀɄ2ܕʗɼʯ
30/150 rxns ¥ 25,000/¥ 100,000
M-MuLV Reverse Transcriptaseɬ࠸ɚ > 5 kbɈcDNAɬਗ
dNTPsĂʂ˂ʌdTʯˁɼʶĜĂˁˋʘʸʯˁɼʶĜʷʛʇʑɬ࠸ɚ
AMV First Strand cDNA Synthesis Kit (#E6550S)
ࢤ౿࿚შćProtoScript AMV First Strand cDNA Synthesis Kit
၆ᆀɄ2ܕʗɼʯ
30 rxns ¥ 28,000
ݗཱܾȦྼᅟɄਲɄʞˋʯ˄ĜʠȥɣɈcDNAਗȦݢ༑ > 10 kbɈcDNAɬਗ
dNTPsĂʂ˂ʌdTʯˁɼʶĜĂˁˋʘʸʯˁɼʶĜʷʛʇʑɬ࠸ɚ ਊ೧ඍ࿚
ProtoScript II Reverse Transcriptase (#M0368S/L/X)
ࢤ౿࿚შćM-MuLV Reverse Transcriptase (RNase H
-)
RNase H–Īʶɼʢʑīळ M-MuLVࢍ๖ଖਊ೧
4,000/ 10,000/ 40,000 units
¥ 9,500/ ¥ 19,000/ ¥ 64,000
൏༂౯Ȧ௫ RNase Hߤ౯ɬฝঌ 37 - 50ŎɁཱܾݢ༑
M-MuLV Reverse Transcriptase (#M0253S/L)
ᄘ౺ळM-MuLVࢍ๖ଖਊ೧
10,000/ 50,000 units
¥ 11,000/ ¥ 44,000
ᅕęɄʞˋʯ˄Ĝʠɬᆵࣅȩࢍ๖ଖ 37 - 45ŎɁཱܾݢ༑
AMV Reverse Transcriptase (#M0277S/L/T)
37 - 52ŎɈဍঢ়ȞཱܾݗɬᄵȳɥᆵࣅȞࢍ๖ଖਊ೧ 200/ 1,000/ 500 units
¥ 12,000/ ¥ 48,000/ ¥ 26,500
ݗཱܾȦྼᅟɄʞˋʯ˄ĜʠɅᅙݢ༑
൦1੍cDNAɈPCRഝဍɅȾȞɀɉ൦2ɬઞ
RT-PCRʅʛʠɉ92 - 93ʲĜʐɬઞ
ProtoScript
®II Reverse Transcriptase
#M0368S 4,000 units . . . . .¥ 9,500 #M0368L 10,000 units . . . .¥ 19,000 #M0368X 40,000 units . . . .¥ 64,000
ತცćProtoScript II Reverse Transcriptaseɉ˂ ʋˋʫʢˋʠM-MuLVࢍ๖ଖਊ೧ɁȜɤĂRNase Hߤ౯ȦฝঌȯɦĂ൏༂౯Ȧ௫ȯɦɀȞɥăᄘ ౺ळM-MuLVɢɤɜȞݗɁ൦1੍cDNAਗ ɬȠȭɂȦɁȧɥăႨਊ೧ɉ50ŎɘɁߤ౯ɬ ။ȾȭɂȦɁȧɥȹɛĂɢɤȞູڽ౯ɂcDNA ਗେᆲɬȱĂ12 kbɘɁɈࠊෳcDNAɈਗ ɬ Ƞ ȭ ɂ Ȧ Ɂ ȧ ɥă Ⴈ ౿ ࿚ Ɉ ࢤ შ ɉ M-MuLV Reverse TranscriptaseĪRNase H–
ī ɁȜɥă
ᄻᅰćM-MuLV Reverse TranscriptaseĪRNase
H–īɬʋĜʡȳɥۆ๙ાɬ෬ࣳ໘ɁཡএȱĂ
ඍێɅɄɥɘɁ౼౿ă
ཱܾ௸ॣć1X ProtoScript II Reverse Transcriptase
Reaction BufferĮ50 mM Tris-HClĪpH 8.3@ 25ŎīĂ75 mM KClĂ3 mM MgCl2įĂ10 mM DTTĂ200 units M-MuLV ĪRNase H–
）Ă0.5 mMɈdNTPsĪ ် ༸Ă#N0447ī ɂ5 ̂MɈ dT23VNĪ်༸Ă#1327īɬ๒ݡă42ŎĂ50ယ࠰ ɼˋʅʽʱĜʏʿˋăˁˋʘʸʯˁɼʶĜɬᅙ ȳɥ௲ਗĂ42ŎɁɈཱܾɈɅଆݗɁ10ယ࠰Ɉ ɼˋʅʽʱĜʏʿˋɬైயȳɥă
༂Ʌɢɥߤ౯ݛć65ŎĂ20ယ࠰
࿚ଋࠣᆅćʠĜʗ˃RNAȥɣ9.2 kbɈcDNAદ
ဘɈਗɬRT-PCRɁߊă
ʾʣʛʠฤࡹć1ʾʣʛʠɉĂཱܾᅗܕ50 ̂l
ෂĂ37ŎĂ10ယ࠰ɁĂʵ˂ĪrAīĆʂ˂ʌĪdTī 18ɬʞˋʯ˄ĜʠʯˁɼʶĜɂȱɀĂ1 nmolɈ dTTPɬબᅗဘଋɅଲɤਮɚȭɂȦɁȧɥਊ೧ ᆲɂȱɀฤࡹȯɦɥăʾʣʛʠɺʛʓɼ௸ॣɉ www.nebj.jpɬઞă
༏ć200,000 units/ml
࠲ᇦ౿࿚ć
RNase H
#M0297S 250 units . . .¥ 12,000 #M0297L 1,250 units . . .¥ 48,000
Deoxynucleotide SolutionɈێᅿɉ95
ʲĜʐɬઞ
■ྫᆲRNAɢɤৌᆎɈࢍ๖ଖȦݢ༑
■ġ50ŎɁཱܾݢ༑
■> 10 kbɈcDNAਗȦݢ༑
SuperScript IIɂ Ɉ ວ ຑ ࿚ Ɂȳă
RN
A研究用試薬
cDNA Synthesis
M-MuLV Reverse Transcriptase
#M0253S 10,000 units . . . .¥ 11,000 #M0253L 50,000 units . . . .¥ 44,000
ತცćMoloney Murine Leukemia Virus ĪM-MuLVī Reverse TranscriptaseɉRNAگശ౯DNAʵ˂ʹ ˁĜʔɁȜɥăȭɈਊ೧ɉʯˁɼʶĜശ੭ݚɅȤ ȞɀĂRNAɘȹɉssDNAɬʞˋʯ˄Ĝʠɂȱɀ DNAഊၒ੍ɬਗȳɥȭɂȦɁȧɥĪRNAɈ௲ਗĂ cDNAਗīăɄȤĂ3ŌŨ 5Ōʀʅʕʤʇ˄ɺĜʔ ߤ౯ɉ૩ȹɄȞă
ᄻᅰćM-MuLVᄻᅰɈࢍ๖ଖਊ೧ۆ๙ાɬᄵȳ ɥ෬ࣳȥɣ౼౿ă
ཱܾ௸ॣć1X M-MuLV Reverse Transcriptase
R e a c t i o n B u ff e rĮ5 0 m M Tr i s - H C lĪp H 8.3@25ŎīĂ75 mM KClĂ3 mM MgCl2Ă10 mM DTTįădNTPĪ ် ༸Ă#N0447ī ɬ ๒ ݡă 37 - 42ŎɁɼˋʅʽʱĜʏʿˋă
༂Ʌɢɥߤ౯ݛć65ŎĂ20ယ࠰
࿚ଋࠣᆅćRNaseĂʀˋʡʤʇ˄ɺĜʔȤɢɍ
ʀʅʕʤʇ˄ɺĜʔȦॳȯɦɄȞȭɂɬߊă
ʾʣʛʠฤࡹć1 unitɉĂpolyĪrAīŜ oligoĪdTī
ɬʞˋʯ˄ĜʠʯˁɼʶĜɂȱɀĂ37ŎĂ10ယ ࠰ɁĂ1 nmolɈdTTPɬબ౯ᅗဘଋɅଲɤਮɚ ȭɂȦɁȧɥਊ೧ᆲɂȳɥăʾʣʛʠɺʛʓɼ ௸ॣɉwww.nebj.jpɬઞă
༏ć200,000 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
■cDNAਗ
■RNAʏĜʉˋʏˋʈ
■RT-PCR
AMV Reverse Transcriptase
#M0277S 200 units . . . . .¥ 12,000 #M0277L 1,000 units . . . . .¥ 48,000
༏࿚Ī2.5Xī
#M0277T 500 units . . . . .¥ 26,500
ತცćAvian Myeloblastosis VirusĪAMVīReverse TranscriptaseɉRNAگശ౯DNAʵ˂ʹˁĜʔɁ ȜɥăȭɈਊ೧ɉʯˁɼʶĜശ੭ݚɅȤȞɀĂ RNAɘȹɉssDNAɬʞˋʯ˄Ĝʠɂȱɀഊၒโ DNA੍ɬਗȳɥȭɂȦɁȧɥĪRNAɈ௲ਗĂ cDNAਗīă
ᄻᅰćAvian Myeloblastosis VirusĪAMVīă
ཱ ܾ ௸ ॣć1X AMV Reverse Transcriptase
Reaction BufferĮ50 mM Tris-HClĪpH 8.3@ 25ŎīĂ75 mM KOAcĂ8 mM MgĪOAcī2Ă10 mM DTTįădNTPĪ ် ༸Ă#N0447ī ɬ ๒ ݡă 37 - 42ŎɁɼˋʅʽʱĜʏʿˋă
࿚ଋࠣᆅćRNaseĂʀˋʡʤʇ˄ɺĜʔȤɢɍ
ʀʅʕʤʇ˄ɺĜʔȦॳȯɦɄȞȭɂɬߊă
ʾʣʛʠɈฤࡹć1 unitɉĂpolyĪrAīŜ oligoĪdTī
ɬʞˋʯ˄ĜʠʯˁɼʶĜɂȱɀĂ37ŎĂ10ယ ࠰ɁĂ1 nmolɈdTTPɬબ౯ᅗဘଋɅଲɤਮɚ ȭɂȦɁȧɥਊ೧ᆲɂȳɥăʾʣʛʠɺʛʓɼ ௸ॣɉwww.nebj.jpɬઞă
༏ć10,000Ȥɢɍ25,000 units/ml
။ശ௫Ɉڷćސষɉ–20ŎɅɀ။ശăय़ᅃ
ސɬटɤ၄ȳɂĂਊ೧Ȧߤ౯ݛȳɥăෳࡖ࠰ Ɉ။ശɅɉĂယষ–70ŎɁɈ။ശɬైயă
ઞ৽ဦॷćwww.nebj.jpɬઞ
■ cDNAਗ
■ RNAʏĜʉˋʏˋʈ
■ RT-PCR
ProtoScript II First Strand cDNA Synthesis Kit
#E6560S 30 rxns . . . . .¥ 18,000 #E6560L 150 rxns . . . . .¥ 72,000
ತცćProtoScript II First Strand cDNA Synthesis KitɉĂ2Ⱦ Ɉ ไ ݛ ȯ ɦ ȹ ਼ ਗ ܕ Ɂ Ȝ ɥ ProtoScript II Enzyme MixȤɢɍProtoScript II Reaction MixɬູɂȱɀȞɥăProtoScript II E n z y m e M i xɉP r o t o S c r i p t I I R e v e r s e TranscriptaseɂMurine RNase InhibitorɈ ਼ ਗ ܕɁȜɤĂProtoScript II Reaction MixɉdNTPs ɂಮᅙʨʛʭɹĜɬ࠸ɚăProtoScript II Reverse TranscriptaseɉM-MuLVࢍ๖ଖਊ೧ɈRNase H ߤ౯ɬฝঌȱĂ൏༂౯ɬ௫ȯȵȹ˂ʋˋʫʢˋ ʠਊ೧ɁȜɥăᄘ౺ळM-MuLVɢɤɜȞݗ Ɂ൦1੍cDNAਗɬȠȭɂȦɁȧɥăႨਊ೧ ɉ50ŎɘɁߤ౯ɬ။ȾȭɂȦɁȧɥȹɛĂɢɤ Ȟູڽ౯ɂcDNAਗେᆲɬȳ
ႨʅʛʠɅɉࢍ๖ଖɈȹɛɈ2ହᇆɈไʯˁɼ ʶĜɂʤʇ˄ɺĜʔʭ˂ĜȦ࠸ɘɦɀȞɥă ɺˋʃĜʂ˂ʌdTʯˁɼʶĜĮdĪTī23 VNįɉ polyAʞĜ˃Ɉާ๘ɅɺʣĜ˃ȳɥăɘȹไ ݛȯɦȹRandom Primer MixɉRNAʞˋʯ˄Ĝ ʠĪmRNAɘȹɉpolyAʞĜ˃ɬ૩ȹɄȞRNAī ɈൌɅȽɀࣧێɅʯˁɼʷˋʈȳɥȭɂȦ Ɂȧɥă10 kbڬ௫Ɉ൦1੍cDNAȦਗݢ༑Ɂ Ȝɥă
ProtoScript II First Strand cDNA Synthesis KitɈ໘ᅎć
— 10X ProtoScript II Enzyme Mix — 2X ProtoScript II Reaction Mix
— Random Primer MixĪ60 µMīĂOligo dĪTī23 VN PrimerĪ50 µMī
— Nuclease-free Water
** Oligo dĪTī23 VNɂRandom Primer Mixɉ1 mM dNTPɬ࠸ɚ
ᅕęɄDNAʞˋʯ˄Ĝʠɬᆵࣅȩഝ ဍȳɥȹɛĂOneTaq®ȜɥȞɉQ5®
High-Fidelity DNA PolymeraseɈ ᅙɬȤೆɛȱɘȳă
࠲ᇦ౿࿚ć
polyA Spin mRNA Isolation Kit
#S1560S 8 isolations . . .¥ 34,000
Magnetic mRNA Isolation Kit
#S1550S 25 isolations . . .¥ 54,000
RNase Inhibitor, Murine
#M0314S 3,000 units . . .¥ 12,000 #M0314L 15,000 units . . .¥ 48,000
Random Primer Mix
#S1330S 100 µl . . .¥ 16,000
Oligo d(T)23VN*
#S1327S 1.0 A260 units . . .¥ 15,500
ŖʹʺćV = AĂGĂCĂN = AĂOĂCĂT
■ Enzyme MixɂReaction MixɅ ɢ ɤĂࠤඍɅཱܾʓʛʠɺʛʯȦݢ༑
■ ȜɣɠɥPCRཱܾɂɈ೨əਗɩȵ
Ȧݢ༑
■ ྫᆲRNAɢɤৌᆎɈࢍ๖ଖȦݢ༑
■ > 10 kbɈcDNAਗȦݢ༑
■ ၆ᆀɄ2ܕʗɼʯ
173
RN
A研究用試薬
ProtoScript First Strand cDNA Synthesis Kit
#E6300S 30 rxns (20 µl vol) . . . ¥ 25,000 #E6300L 150 rxns (20 µl vol) . .¥ 100,000
ತ ცćProtoScript M-MuLV First Strand cDNA Synthesis KitɉĂ2ȾɈไݛȯɦȹ਼ਗܕɁȜ ɥProtoscript Enzyme MixɂProtoscript Reaction MixɬູɂȱɀȞɥăProtoscript Enzyme Mix ɉM-MuLV Reverse TranscriptaseɂRNase Inhibitor, MurineɈ਼ਗܕɁȜɤĂProtoscript Reaction MixɉdNTPɂಮᅙʨʛʭɹĜɬ࠸ɚă ɘȹĂႨʅʛʠɉ2ହᇆɈࢍ๖ଖᅙɅไݛȯɦ ȹʯˁɼʶĜɂʤʇ˄ɺĜʔʭ˂Ĝɬ࠸ɚă ɺˋʃĜʂ˂ʌdTʯˁɼʶĜĮdĪTī23 VNįɉ polyAʞĜ˃Ɉާ๘ɅɺʣĜ˃ȳɥăไݛȯ ɦ ȹRandom Primer MixɉĂRNAʞ ˋ ʯ ˄ Ĝ ʠ ĪmRNAɘȹɉpolyAʞĜ˃ɬ૩ȹɄȞRNAīɈ ൌɅȽɀࣧێɅʯˁɼʷˋʈȳɥȭɂȦɁȧ ɥă13.0 kbڬ௫Ɉ൦1੍cDNAȦਗݢ༑ɁȜɥă ႨʅʛʠɈࢤ౿࿚შɉM-MuLV First Strand cDNA Synthesis KitɁȜɥă
ProtoScript M-MuLV First Strand cDNA Synthesis Kit໘ᅎć
— 10X ProtoScript Enzyme Mix — 2X ProtoScript Reaction Mix — Random Primer MixĪ60 ̂Mī — Oligo dĪTī23 VN primerĪ50 ̂Mī** — Nuclease-free Water
** Oligo dĪTī23 VN PrimerɂRandom Primer Mixɉ1 mM dNTPɬ࠸ɚ
ProtoScriptʅʛʠɅɢɥ൦1੍cDNAਗ
2 ̂gɈʪʠ᪷ടʠĜʗ˃ RNAɬᅙȞɀ42ŎɁཱܾ ɬȽȹăʥʄʞɻʮʋˋʠ˅Ĝ˃ཱܾܕĪ-RTī ɉ1X ProtoScript Reaction MixɈəɂȱȹăਗȯ ɦȹ൦1੍ cDNAɈێငɬĂ3ହᇆɈmRNAɅູڽโ Ʉ༯ᇙɅȾȞɀĂ1 X LongAmp® Taq 2X Master MixĪNEB #M0287īɬᅙȞɀഝဍȱȹă˄Ĝˋ1ć ʱĜʗɺʇʙˋĪ1.1 kbīĂ˄Ĝˋ2ćʥʄʞɻʮʋ ˋʠ˅Ĝ˃ĪʱĜʗɺʇʙˋīĂ˄Ĝˋ3ćXrn-1Ī4.7 kbīĂ˄Ĝˋ4ćʥʄʞɻʮʋˋʠ˅Ĝ˃ĪXrn-1īĂ ˄Ĝˋ5ćʈɺʣˋʤʇ˄ʂʙʡʀʇʑʙɿˋʐ ʭɹʇʗĜp532Ī9.8 kbīĂ˄Ĝˋ6ćʥʄʞɻʮʋ ˋ ʠ ˅ Ĝ ˃Īp532īĂ ယ ા ᆲ ʶ Ĝ ʃ ĜMć2-Log DNA LadderĪNEB #N3200īă
࠲ᇦ౿࿚ć
polyA Spin mRNA Isolation Kit
#S1560S 8 isolations . . .¥ 34,000
Magnetic mRNA Isolation Kit
#S1550S 25 isolations . . .¥ 54,000
■ Enzyme MixɂReaction MixɅ ɢ ɤĂࠤඍɅཱܾʓʛʠɺʛʯȦݢ༑
■ ȜɣɠɥPCRཱܾɂɈ೨əਗɩȵ
Ȧݢ༑
■ ྫᆲRNAɢɤৌᆎɈࢍ๖ଖȦݢ༑
■ வɄȩɂɜ5 kbɈcDNAਗȦݢ༑
AMV First Strand cDNA Synthesis Kit
#E6550S 30 reactions . . . . .¥ 28,000 ತცćAMV First Strand cDNA Synthesis KitɉĂ
AMV Enzyme MixɂAMV 2X Reaction MixȥɣɄ ɥ ăA M V E n z y m e M i xɉA M V R e v e r s e TranscriptaseɂRNase Inhibitor, MurineɈ ไ ݛ ȯɦȹ਼ਗܕɁȜɥăAMV Reverse Transcriptase Ʌɢɥ൦1੍cDNAਗཱܾɈไݗཱྀڱɉঢ়ȩĂ 37 - 50Ŏ Ɂ ᅙ ݢ ༑ Ɂ Ȝ ɥăMurine RNase InhibitorɉʪʠᄻᅰRNase೫ޮ੬ɂɓɀબݛȯ ɦɅȩȩĂRNAʞˋʯ˄Ĝʠɬɢɤێ။ীȳɥ ȭɂȦɁȧɥăAMV 2X Reaction MixɉdNTPɬ ࠸ɚไݛȯɦȹʨʛʭɹĜɁȜɥă1X AMV Reaction Mixശ੭ݚɁAMV Enzyme Mixɉ10 kb ɘ Ɂ Ɉ ෳ ȯ ɈcDNAɬ େ ᆲ Ɂ ਗ ȳ ɥă Ⴈ ʅ ʛ ʠ Ɉ ࢤ ౿ ࿚ შ ɉProto Script AMV First Strand cDNA Synthesis KitɁȜɥă
AMV First Standard cDNA Synthesis Kit໘ᅎć
— 10X AMV Enzyme Mix — AMV 2X Reaction Mix — Random Primer MixĪ60 ̂Mī — Oligo dĪTī23 VN PrimerĪ50 ̂Mī** — Nuclease-free Water
** Oligo dĪTī23 VN PrimerɂRandom Primer Mixɉ1 mM dNTPɬ࠸ɚ
AMV Enzyme MixɅɢɤਗȯɦȹ൦1੍cDNA દဘɉݚᆔɈۆ๙ાʇ˅ĜʣˋʈɈ഼ĂPCRɞ ˂ɺ˃ʗɼʸPCRɅɢɥฤᆲယɅไȱɀȞɥă
60.0 58.2 55.3 51.0 45.5 41.4 38.6 37.0 kb
0.9
5.8
9.8
Temp (°C)
AMVɈݗไਗ౯ć2 unitɈAMVɬᅙȞĂ20 ̂lɈ
ཱܾᅎᆲɁĂ1 ̂gɈʪʠ᪷ടʠĜʗ˃ RNAȥɣ൦ 1੍cDNAਗɬȽȹăూෂɅȱȹݗɁ1૪࠰ ཱܾɬȞĂcDNAદဘɈᄝ10ယɈ1ᆲɬʞˋʯ˄Ĝ ʠɂȱɀLongAmp® Taq 2X Master MixɅɢɤഝဍ ȱȹăʈɺʣˋʀʇʑʙɿˋʐʭɹʇʗĜp532Ī15.14 kbīɈȠȻĂഝဍȱȹᆴۊɬూෂɅȱȹă
࠲ᇦ౿࿚ć
polyA Spin mRNA Isolation Kit
#S1560S 8 isolations . . .¥ 34,000
Magnetic mRNA Isolation Kit
#S1550S 25 isolations . . .¥ 54,000
■ Ȟཱܾݗɬྼᅟɂȳɥഝဍਲ
Ʉʞˋʯ˄ĜʠɅไ
■ cDNAદဘɉPCRĂʇ˅ĜʣˋʈĂ ˂ɺ˃ʗɼʸPCRɄɃɅᅙݢ༑
Primers for cDNA Synthesis
ʂ˂ʌdĪNīn ʯˁɼʶĜɉĂ3Ō-polyAʞĜ˃Ȧ ݡȱȹmRNAȜɥȞɉʞĜ˂ˋʈcDNAɈʯˁ ɼʷˋʈȤɢɍʏĜʉˋʏˋʈɅᅙȯɦɥă
ʹʺć#S1316Ʌɉ5Ō˂ˋબȦݡȯɦɀȞɄȞă
ઞ৽ဦॷćwww.nebj.jpɬઞ
౿࿚ ౿࿚ྈਖ ᅎᆲ ࡏႊழ༸ݟ߆
Random Primer 6 (5´d(N6)3´) ~14.6 nmol S1230S 1.0 A260 units ¥ 16,000
Random Primer 9(5´d(N9)3´) ~11.6 nmol S1254S 1.0 A260 units ¥ 16,000
Oligo d(T) VN S1327S 1.0 A units ¥ 15,500
1 2 3 4 5 6 M
10.0 8.0 6.0 5.0 4.0 3.0
2.0
1.5 1.2 1.0 0.9 0.8 0.7 kb
RN
A研究用試薬
RNA Ligases
NEBɁɉDNAɂRNAॸ࢞ɁᅙȳɥହęɈ˂ʄĜʔɬอࢷȱɀȞɥăȭɦɣɈਊ೧Ɉഽȩɉ˂ ʋˋʫʢˋʠʗˋʩʇଋɁȜɤĂ഼ɈNEB౿࿚ɂວᅕɅ࿚ଋȤɢɍఒᅲ౯ȦȞăڬݚɈూɅ ߂T4˂ʄĜʔɈߤ౯ɬɺʯ˂ʉĜʏʿˋ်ɅȱɀȞɥăDNA˂ʄĜʔɈೊ൰ʙʻĜʠɉ100 ʲĜʐɅɜࡥ੪ȱɀȞɥă
T4
˂ʄĜʔɈߤ౯Ȥɢɍɺʯ˂ʉĜʏʿˋOH P
T4 RNA Ligase 1
1
OH pNp
pdNp 2
OH 3
5
OH P App
4 OH P
7 OH
6
OH P
2
OH P 1
OH P
T4 RNA Ligase 2
3
4
OH P OH P
T4 RNA Ligase 2 Truncated T4 RNA Ligase 2 Truncated K227Q
T4 RNA Ligase 2 Truncated KQ
1
OH 2
OH App
App
OH
3 App
T4 DNA Ligase
1
2
OH P
OH P
Thermostable 5´ App DNA/RNA Ligase
1
2
3
App
OH App OH
OH App
5´ Adenylation Kit
1
2
Appss DNA 5´ Pss DNA
App RNA 5´ P RNA
RNA DNA
௫ࡥɈߤ౯ɬຶɥɅɉཱܾ௸ॣɈไݛȦྼᅟɄ௲ਗȦȜɥă
RNA
˂ʄĜʔೊ൰ʙʻĜʠT4 RNA
Ligase 1 T4 RNALigase 2
T4 RNA Ligase 2 Truncated
T4 RNA Ligase 2, Truncated
K227Q
T4 RNA Ligase 2, Truncated
KQ
Thermostable 5ŌAppDNA/
RNA Ligase 5
ŌAdenylation Kit
RNAɺʯ˂ʉĜʏʿˋ 2Ⴈ੍RNAෂɈ
ʣʛʇˁɼʊĜʏʿˋ
★ ★ ★
RNA3ŌჂɈˁʱ˂ˋʈ
★ ★ ★
★
★
★
★
1Ⴈ੍RNAɈˁɼʊĜʏʿˋ
★ ★ ★
ɺʟʣ˃ݛɺʘʯʗĜɈRNAɒɈˁɼʊĜʏʿˋ
★ ★
★ ★
★ ★
★ ★ ★
★ ★
5Ōɺʟʣ˃ݛ
★ ★ ★
DNAɺʯ˂ʉĜʏʿˋ
ɺʟʣ˃ݛɺʘʯʗĜɈ
1Ⴈ੍DNAɒɈˁɼʊĜʏʿˋ
★ ★ ★
DNA / RNAɺʯ˂ʉĜʏʿˋ 2Ⴈ੍৭ഢɅȤȫɥDNAȤɢɍ
RNAɈय़ਗ
★ ★
NGSɺʯ˂ʉĜʏʿˋ
NGSˁɼʮˁ˂Ĝ෮౿
▲
▲
▲
▲
1Ⴈ੍RNAɂ1Ⴈ੍DNAɈ
ˁɼʊĜʏʿˋ
▲
ູ
൏༂౯ ✔ ✔
˂ʋˋʫʢˋʠ ✔ ✔ ✔ ✔ ✔ ✔ ✔
★ ★ ★ ไĂȷɈɺʯ˂ʉĜʏʿˋɅైயȯɦɥ˂ʄĜʔ
★★ ȷɈɺʯ˂ʉĜʏʿˋɁᆯɅᅙݢ༑
★ ȷɈɺʯ˂ʉĜʏʿˋɅᅙɁȧɥȦĂైயȯɦɄȞ
▲ ไɄਊ೧ɬೊ൰ȳɥȹɛɅɉNGSɈʯ˅ʠʋĜ˃ɬઞ৽Ʌȳɥ
T4RNARN
175
RN
A研究用試薬
6
T4 RNA Ligase 1
Ī
ssRNA Ligase
ī
#M0204S 1,000 units . . . . .¥ 10,500 #M0204L 5,000 units . . . . .¥ 42,000
ತცć3ŌŨ 5Ōʳʑʳʐʀʑʞ˃य़ਗɬवȳɥ ȭ ɂ Ʌ ɢ Ƚ ɀĂ ߇ બ Ɉ5ŌჂ Ɉ ˂ ˋ બ ࡊĪ ʡ ʢĜīɬ3ŌჂɈʪʡ˅ʅʏࡊĪɺʇʓʯʗĜī ɅˁɼʊĜʏʿˋȳɥཱܾɬఌ༲ȳɥăȷɈ੫Ă ATPȦAMPɂPPiɅݡယސȯɦɥăࡊଋɅɉ1 Ⴈ੍ɈRNAɂDNAĂʐʤʇ˄ʂʏʡʬ˅˂ˋબ Ȧ࠸ɘɦɥă
ᄻᅰćT4 RNA Ligase 1ۆ๙ાɬᄵȳɥ෬ࣳă
ཱܾ௸ॣć1X T4 RNA Ligase Reaction Buffer
Į50 mM Tris-HClĪpH 7.8īĂ10 mM MgCl2Ă1 mM DTTįĂ1 mM ATPɬ ݡ Ȣ37Ŏ Ɂ ɼ ˋ ʅ ʽ ʱĜʏʿˋă
༂Ʌɢɥߤ౯ݛć65ŎɁ15ယ࠰Ăɜȱȩɉʴ ɼ˃ȱɀ2ယ࠰
ᅙ௫ɈڷćpCpɈˁɼʊĜʏʿˋɅɉDMSO
ɬ10%Īv/vī๒ݡȳɥྼᅟȦȜɥă
മᄞć
10X T4 RNA Ligase Reaction Buffer 10 mM ATP
50% PEG 8000
࿚ଋࠣᆅć1Ⴈ੍DNAʀʅʕʤʇ˄ɺĜʔĂʀˋ
ʡʤʇ˄ɺĜʔĂRNaseĂʳʑʭɹʗĜʔɈʋˋ ʗʷʥĜʏʿˋȦჟȞȭɂɬߊă
ʾʣʛʠฤࡹć1unitɉĂ37ŎĂ30ယ࠰ɁĂ1 nmol
Ɉ5Ō-Į32PįrA16ɬʳʑʭɹʗĜʔ൏౯ळĪɾɿ ʮȥɣīɅဿࠓȳɥɈɅྼᅟɄਊ೧ᆲɂȱɀฤ ࡹȯɦɥăʾʣʛʠɺʛʓɼɈଌঅ௸ॣɉwww. nebj.jpɬઞă
༏ć10,000 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
࠲ᇦ౿࿚ć
Adenosine 5Ō - Triphosphate (ATP)
#P0756S 1 ml . . . .¥ 5,000
#P0756L 5 ml . . .¥ 20,000
Universal miRNA Cloning Linker
#S1315S 0.83 nmol . . .¥ 36,000
■ 1Ⴈ ੍RNAȤ ɢ ɍDNAɈ ˁ ɼ ʊ Ĝ ʏʿˋ
■ RNAɈ3Ō-Ⴢɬ5Ō-Į32PįpCpɁ ࿈ૻ
■ RNAɂDNAɈယા໘Ăယા࠰ᇦय़
■ 1Ⴈ੍ʂ˂ʌʟʂʅʏ˂ʴʤʇ˄ʂ ʙʡɈਗ
■ ྥ๏ɺʷʦબɈʗˋʩʇଋɒɈ
ଲɤਮə
T4 RNA Ligase 2
Ī
dsRNA Ligase
ī
#M0239S 150 units . . . .¥ 13,500 #M0239L 750 units . . . .¥ 54,000
ತ ცćT4 RNA Ligase 2ɉT4 Rnl-2Īgp24.1ī ɂɜঘɊɦĂRNAɈယા࠰Ȥɢɍယા໘Ɉᆤၫ Ɉᇦय़ߤ౯ɬᄵȳɥăT4 RNA Ligase 1ĪNEB #M0204īɂɉڽɄɤĂT4 RNA Ligase 2ɉĂ1 Ⴈ੍RNAɈჂɬय़ਗȳɥߤ౯Ʌɓɀ2Ⴈ੍
RNAɈʣʛʇɬय़ਗȳɥߤ౯Ȧྥ௵ɅȞăȭ
Ɉਊ೧ɅɢɥˁɼʊĜʏʿˋɁɉĂ5Ō˂ˋબࡊ ɂ3Ōʪʡ˅ʅʏࡊȦᆿಞȱɀȞɥྼᅟȦȜɥă ȭ Ɉ ਊ ೧ ɉĂRNAɈ3Ō ʪ ʡ ˅ ʅ ʏ ࡊ ɬ2Ⴈ ੍ DNAɈ5Ō˂ˋબࡊɅˁɼʊĜʏʿˋɁȧɥă
ᄻᅰćT4 RNA Ligase 2ۆ๙ાɬᄵȳɥ෬ࣳă
ཱ ܾ ௸ ॣć1X T4 RNA Ligase 2 Reaction
BufferĮ50 mM Tris-HClĪpH 7.5Ă25ŎīĂ2 mM MgCl2Ă1 mM DTTĂ400 µM ATPįă37Ŏ ɁɼˋʅʽʱĜʏʿˋă
࿚ଋࠣᆅć1Ⴈ੍Ȥɢɍ2Ⴈ੍DNAʀʅʕʤʇ˄
ɺĜʔĂʀˋʡʤʇ˄ɺĜʔĂRNaseĂʳʑʭɹ ʗĜʔɈʋˋʗʷʥĜʏʿˋȦჟȞȭɂɬߊă
ʾʣʛʠฤࡹć1 unitɉĂ37ŎĂ30ယ࠰ɁĂ20
̂lɈ ཱ ܾ ᅗ ܕ ෂ Ʌ ശ ੭ ȳ ɥ0.4 ̂gɈ23-mer RNAȤɢɍ17-mer RNAɈຑʺ˃਼ਗဘɬˁɼ ʊĜʏʿˋȳɥɈɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯ ɦɥă
༏ć10,000 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
■ໃჂɺʘʯʗĜˁɼʊĜʏʿˋ
■dsRNAෂɈʣʛʇɈˁɼʊĜʏʿˋ Ʌไ
■RNAɈ3Ōʪʡ˅ʅʏჂɬDNAɈ5Ō ˂ ˋ બ Ʌ ˁ ɼ ʊ Ĝ ʏ ʿ ˋ ȱ ɀDNA/ RNAʧɼʮ˂ʛʡɬ౿ȳɥɈɅไ
T4 RNA Ligase 2, truncated
#M0242S 2,000 units . . . . .¥ 11,500 #M0242L 10,000 units . . . . .¥ 46,000
ತცćT4 RNA Ligase 2, truncatedĪT4 Rnl2 truncatedī ɉĂ ɺ ʟ ʣ ˃ ݛ ȯ ɦ ȹDNAɘ ȹ ɉ RNAɈ5ŌჂ ɬRNAɈ3ŌჂ Ʌ ູ ڽ โ Ʌ ˁ ɼ ʊĜʏʿˋȳɥăȭɈਊ೧ɉˁɼʊĜʏʿˋɅ ATPɬྼᅟɂȱɄȞȦĂࡊଋɬૢɅɺʟʣ˃ ݛȱɀȤȩྼᅟȦȜɥăT4 Rnl2 truncatedɉĂ T4 RNA Ligase 2ɈࠊෳɈɈ249ɺʷʦબ ɬʋĜʡȳɥʯˁʑʷʡɬ෬ࣳ໘Ɂཡএȯȵ ȹɜɈɁȜɥăࠊෳɈ˂ʄĜʔɂڽɄɤĂT4 Rnl2 truncatedɉࡊଋɈ5ŌჂɬɺʟʣ˃ݛɁ ȧɄȞȹɛĂRNAɘȹɉDNAɈ˂ˋબݛ5ŌჂ ɬRNAɈ3ŌჂ Ʌ ˁ ɼ ʊ Ĝ ʏ ʿ ˋ Ɂ ȧ Ʉ Ȟă Rnl2Ī1-249īɂɜঘɊɦɥȭɈਊ೧ɬᅙȞȹ ˂ˋʃĜˁɼʊĜʏʿˋɉĂmicroRNAʇ˅Ĝ ʣˋʈɅไɁȜɥăȭɈਊ೧ɉɺʟʣ˃ݛȯɦ ȹʯˁɼʶĜȺȫɬࡊଋɂȳɥɈɁĂˁɼʊĜ ʏʿˋɈʨʛʇʈˁɾˋʡɬঌɣȳȭɂȦɁȧɥă
ᄻᅰćT4 Rnl2 truncatedۆ๙ાɬᄵȳɥ෬ࣳă
ཱܾ௸ॣć1X T4 RNA Ligase Reaction Buffer
Į50mM Tris-HClĪpH 7.5Ă25ŎīĂ10 mM
മᄞć
10X T4 RNA Ligase Reaction Buffer 50% PEG 8000
࿚ଋࠣᆅć1Ⴈ੍Ȥɢɍ2Ⴈ੍DNAɈʀʅʕʤʇ
˄ɺĜʔĂʀˋʡʤʇ˄ɺĜʔĂRNaseĂʳʑ ʭɹʗĜʔߤ౯ɈʋˋʗʷʥĜʏʿˋȦჟȞȭ ɂɬߊă
ʾʣʛʠฤࡹć200 unitɉĂ25ŎĂ1૪࠰ɁĂ10
̂lɈཱܾᅗܕෂɅശ੭ȳɥ31-merɈRNAɂ17-merɈɺʟʣ˃ݛჂɬ80%ˁɼʊĜʏʿˋȳɥ ɈɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦɥă
༏ć200,000 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
࠲ᇦ౿࿚ć
Universal miRNA Cloning Linker
#S1315S 0.83 nmol . . .¥ 36,000
■ ɺʟʣ˃ݛȱȹRNAɘȹɉDNAɬ ȜɣɠɥRNAɈ3ŌჂɅˁɼʊĜ ʏʿˋ
■ 1Ⴈ੍ɺʟʣ˃ݛʯˁɼʶĜɬฝယ ાRNAɅ य़ ਗ ȱ ɀcDNAˁ ɼ ʮ ˁ ˂Ĝɬ౿
■ 1Ⴈ ੍ ɺ ʟ ʣ ˃ ݛ ʯ ˁ ɼ ʶ Ĝ ɬ RNAɅय़ਗȱɀ੍ູڽโɄcDNA ˁɼʮˁ˂Ĝɬ౿
RN
A研究用試薬
T4 RNA Ligase 2, truncated K227Q
T4 RNA Ligase 2, truncated KQ
T4 RNA Ligase 2, truncated K227Q #M0351S 2,000 units . . . .¥ 12,000 #M0351L 10,000 units . . . .¥ 48,000 T4 RNA Ligase 2, truncated KQ #M0373S 2,000 units . . . .¥ 12,000 #M0373L 10,000 units . . . .¥ 48,000
ತცćT4 RNA Ligase 2, truncated KQĪT4 Rnl2tr KQīɉĂɺʟʣ˃ݛȯɦȹDNAɘȹɉ RNAɈ5ŌჂɬRNAɈ3Ōʪʡ˅ʅʏჂɅູڽ โ Ʌ ˁ ɼ ʊ Ĝ ʏ ʿ ˋ ȳ ɥă Ⴈ ਊ ೧ ɉ ˁ ɼ ʊ Ĝ ʏʿˋɅATPɬྼᅟɂȱɄȞȦĂࡊଋɬૢɅ ɺʟʣ˃ݛȱɀȤȩྼᅟȦȜɥă
T4 Rnl2tr KQɉĂT4 RNA Ligase 2, truncated ĪNEB #M0242īɈʘʮ˃ʵɼˋʠʷʽĜʗˋ
ʠɁȜɥăT4 RNA Ligase 2ɈK227Ʌဿڽɬຩ ȱȹȭɂɅɢɤĂਊ೧Ɉ˂ʏ˃ɺʟʣ˃ݛȦ ঌவȳɥăK227QɉT4 Rnl2trɅɢɥႊɘȱȩɄ Ȟ ˁ ɼ ʊ Ĝ ʏ ʿ ˋ દ ဘĪ ʋ ˋ ʃ ʗ ʶ Ĝ ɞ ࠞ DNAīɬঌவȯȵɥăȭɦɉ๒ݡȱȹRNAɈ5ħ ˂ˋબɅT4 Rnl2trɈ˂ˋʃĜȥɣɺʟʣ˃ࡊɬ ๖ھȳɥߤ౯ȦঌவȳɥȭɂɅɢɥăT4 Rnl2tr K227QɈR55KɈဿڽɉਊ೧ɈˁɼʊĜʏʿˋ ߤ౯ɬT4 Rnl2trɂວาɘɁ௫ȱɀȞɥă
ATPɈ༧தĂɺʟʣ˃ݛȱȹ˂ˋʃĜɈᅙĂ ȷɦɅਊ೧Ɉ˂ʏ˃ɺʟʣ˃ݛߤ౯ɈঌவɅ ɢȽɀĂˁɼʊĜʏʿˋཱܾɈʨʛʇʈˁɾˋ ʡȦழݛȳɥăȭɈਊ೧ɉsmall RNAɈʧɼ ʑ˃ĜʯʛʠɄʏĜʉˋʑˁɼʮˁ˂ĜɈ ȹɛɈไݛȯɦȹ˂ˋʃĜˁɼʊĜʏʿˋɅ ᅙȳɥȭɂȦɁȧɥă
ᄻᅰćࠊෳɈT4 RNA Ligase 2ɈɈ249ɺ ʷʦબɬʋĜʡȳɥʯˁʑʷʡȥɣMBPᅃਗʗˋ ʩʇଋɂȱɀ෬ࣳ໘ɁཡএăT4 Rnl2tr K227Q ɉĂ227ྈᄆɈ˂ʏˋɬʈ˃ʗʷˋɅࠓȳɥဿ ڽɬຩăT4 Rnl2tr KQɉĂ55ྈᄆɈɺ˃ʆʣ ˋɬ˂ʏˋɅĂ227ྈᄆɈ˂ʏˋɬʈ˃ʗʷˋɅ ࠓȳɥဿڽɬຩă
ཱܾ௸ॣć1X T4 RNA Ligase Reaction Buffer
Į50 mM Tris-HClĪpH 7.5@25ŎīĂ10 mM MgCl2Ă1 mM DTTįă25ŎɁɼˋʅʽʱĜʏʿˋă ༂Ʌɢɥߤ౯ݛć65ŎĂ20ယ࠰
മᄞć
10X T4 RNA Ligase Reaction Buffer 50% PEG 8000
࿚ଋࠣᆅć1Ⴈ੍Ȥɢɍ2Ⴈ੍DNAɈʀʅʕʤʇ
˄ɺĜʔĂʀˋʡʤʇ˄ɺĜʔĂRNaseĂʳʑ ʭɹʗĜʔߤ౯ɈʋˋʗʷʥĜʏʿˋȦჟȞȭ ɂɬߊă
ʾʣʛʠฤࡹć200unitɉĂ25ŎĂ1૪࠰ɁĂ10
̂lɈཱܾᅗܕෂɅശ੭ȳɥ31-merɈRNAɂ17-merɈ ɺ ʟ ʣ ˃ ݛDNAჂ ĮUniversal miRNA Cloning LinkerĪNEB #S1315īįɬ80%ˁɼʊĜ ʏʿˋȳɥɈɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦ ɥăʾʣʛʠɺʛʓɼɈଌঅ௸ॣɉwww.nebj.jp ɬઞă
༏ć200,000 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
࠲ᇦ౿࿚ć
Universal miRNA Cloning Linker
#S1315S 0.83 nmol . . .¥ 36,000
■ɺʟʣ˃ݛȱȹRNAɘȹɉDNAɬȜɣ ɠɥRNAɈ3ŌჂɅˁɼʊĜʏʿˋ
■1Ⴈ੍ɺʟʣ˃ݛʯˁɼʶĜɬฝယા RNAɅय़ਗȱɀcDNAˁɼʮˁ˂Ĝɬ ౿
■1Ⴈ੍ɺʟʣ˃ݛʯˁɼʶĜɬRNAɅ य़ ਗ ȱ ɀ ੍ ູ ڽ โ ɄcDNAˁ ɼ ʮ ˁ ˂Ĝɬ౿
Thermostable 5
Ō
AppDNA / RNA Ligase
#M0319S 10 reactions . . .¥ 12,000 #M0319L 50 reactions . . .¥ 48,000
ತცćThermostable 5ŌApp DNA/RNA Ligase ɉĂMethanobacterium thermoautotrophicum
ᄻ ᅰ ɈRNA˂ ʄ Ĝ ʔ Ɉ ఌ ༲ ˂ ʏ ˋ Ɉ ʵ ɼ ˋ ʠ ʷʽĜʗˋʠɁȜɥăႨਊ೧ɉATPྥگശ౯Ɂ ȜɥăRNAɜȱȩɉ1Ⴈ੍DNAɈ3Ōʪʡ˅ʅʏ ჂɒɈˁɼʊĜʏʿˋɅɉĂ5Ōɺʟʣ˃ݛ˂ ˋʃĜɬྼᅟɂȳɥă2Ō-O-ʹʙ˃ݛ3ŌჂɬᄵ ȳ ɥRNAɬ5Ōɺ ʟ ʣ ˃ ݛ ˂ ˋ ʃ Ĝ Ʌ ˁ ɼ ʊ Ĝ ʏʿˋȳɥȭɂɜɁȧɥăˁɼʊĜʏʿˋཱܾ Ɉไݗɉ60 - 65ŎɁȜɥăȭɈဿڽळ˂ ʄĜʔɉRNAɜȱȩɉ1Ⴈ੍DNAɈ5Ō˂ˋબɬ ɺʟʣ˃ݛȳɥȭɂɉɁȧɄȞȹɛĂႊɘȱȩ ɄȞˁɼʊĜʏʿˋદဘĪʋˋʃʞʶĜɞࠞ DNAīɬঌவȯȵɥă
Ⴈ˂ʄĜʔɉ65ŎɁɜߤ౯ɬᄵȳɥȹɛĂRNA ˁɼʊĜʏʿˋଌঅɈႄȬɂɄɥ૫৭ഢव ɬฝঌɁȧɥă
ᄻᅰćThermostable 5ŌApp DNA / RNA Ligase ɉHisʗʈᅃਗʗˋʩʇଋɂȱɀ෬ࣳ໘Ɂཡএă
ཱ ܾ ௸ ॣć1X NEBuffer 1Į10 mM
Bis-Tris-Propane-HClĪpH 7.0@25ŎīĂ10 mM MgCl2Ă 1 mM dithiothreitolįă65ŎɁɼˋʅʽʱĜʏʿ ˋă
࿚ଋࠣᆅć1Ⴈ੍Ȥɢɍ2Ⴈ੍DNAɈʀʅʕʤʇ
˄ɺĜʔĂʀˋʡʤʇ˄ɺĜʔĂRNaseĂʳʑ ʭɹʗĜʔߤ౯ɈʋˋʗʷʥĜʏʿˋȦჟȞȭ ɂɬߊă
༏ć20 ̂M
ʹʺć1Ⴈ੍DNAɬɺʟʣ˃ݛ˂ˋʃĜɅˁɼ ʊ Ĝ ʏ ʿ ˋ ȳ ɥ ȹ ɛ Ʌ ɉĂMn2+
ɬ ๒ ݡ ȱ ȹ NEBuffer 1ɬᅙȳɥȭɂɬైயȳɥă
ઞ৽ဦॷćwww.nebj.jpɬઞ
࠲ᇦ౿࿚ć
Universal miRNA Cloning Linker
#S1315S 0.83 nmol . . .¥ 36,000
■ NGSˁɼʮˁ˂Ĝ෮౿ɅȤȞɀĂ ɺʟʣ˃ݛȱȹDNA˂ˋʃĜɬ1 Ⴈ੍DNAɅˁɼʊĜʏʿˋ
■ Small RNA NGSˁɼʮˁ˂Ĝ෮౿ ɅȤȞɀĂȞݗɁɺʟʣ˃ݛ ˂ˋʃĜɬRNAɅˁɼʊĜʏʿˋ
5
Ō
DNA Adenylation Kit
#E2610S 10 reactions . . . .¥ 19,000 #E2610L 50 reactions . . . .¥ 76,000
ತ ცć5ŌDNA Adenylation KitɁ ɉĂMth RNA ˂ʄĜʔĂATPĂ5Ō-˂ˋબݛȯɦȹ1Ⴈ੍DNA ɬᅙȞɀĂ5Ōɺʟʣ˃ݛʂ˂ʌDNAɬࠤඍȥȾ ৌᆎโɅ౿ȳɥȭɂȦɁȧɥăȭɈʅʛʠɉ3Ō ʗĜʷʥĜʗĜɈᄵჟɅ࠲ɩɣȴĂɺʟʣ˃ݛ DNAෂ࠰ൌɬ౺ɁȧɥɢȠไݛȯɦɀȞɥă 5ŌDNA Adenylation KitɉpDNAȥɣAppDNAɬ 95%ڬ௫ɈဿࠓৌᆎɁ˃ĜʙˋɅ౺Ɂȧɥă ȭɈৌᆎɈʯ˅ʓʑɁɉĂʊ˃ɅɢɥෆɈ ྼᅟȦჟȞȹɛĂൌɈେᆲȦ௫ȳɥă
5ŌDNA Adenylation KitɈ໘ᅎć
—Mth RNA LigaseĪRecombinantī —Ī10Xī5ŌDNA Adenylation Reaction Buffer — 10 mM ATP
࿚ଋࠣᆅćʀˋʡʤʇ˄ɺĜʔɂʀʅʕʤʇ˄
ɺĜʔɈʋˋʗʷʥĜʏʿˋȦჟȞȭɂɬߊă
ʹʺćਊ೧ɈʗĜˋʂĜʨĜȦඵȞɈɁĂਊ೧
ɂʂ˂ʌʤʇ˄ʂʙʡࡊଋ༏ɬȤȤɢȷຑʺ ˃ɅȳɥྼᅟȦȜɥăɺʟʣ˃ݛDNA˂ˋʃĜ ɉĂ૫౦ൣʏĜʉˋʑɈȹɛɈĂcDNAˁɼʮˁ ˂Ĝ৭භ૪ɅȤȫɥRNA 3ŌჂˁɼʊĜʏʿˋ ɅᅙȞɥȭɂȦɁȧɥă
ઞ৽ဦॷć www.nebj.jpɬઞ
■NGSɅȤȞɀĂ1Ⴈ੍DNA˂ˋʃĜɬ ਊ೧โɅ5Ōɺʟʣ˃ݛ
■ˇˋʑʞʛʯɁฤᆲโɅɺʟʣ˃ݛ
■ࡕശɈݛߔโĂਊ೧โၭɢɤɜࠤඍ
■ཱܾદဘɈ౼౿ʑʞʛʯȦᅟ
177
RN
A研究用試薬
6
HEN1 miRNA Methyltransferase
#M0228S 1,000 units . . . .¥ 11,000 #M0228L 5,000 units . . . .¥ 44,000
ತცćHEN1ɉȞ2Ⴈ੍RNAɈჂ˂ʴĜʑɬ ʹʙ˃ݛȳɥఆဘᄻᅰɈmiRNAʹʙ˃ʠˁˋʑ ʭɿˁĜʔɁȜɥăArabidopsisɁɉĂmiRNAȤ ɢɍsiRNAɈ3Ō˂ʴĜʑჂɈ2Ō-O-ʹʙ˃ݛɉ HEN1Ʌ ɢ ɤ ఌ ༲ ȯ ɦ ɥăHEN1ဿ ڽ ߰ Ɂ ɉĂ small RNAɉʹʙ˃ݛȯɦɀȤɣȴĂ3ŌpolyĪUī ʞĜ˃ȦݡȯɦɀȞɥȹɛĂ3ŌჂɈʹʙ˃ ݛɉsmall RNAɬɾ˂ʐ˃ݛȥɣ။ীȱɀȞɥ ɂ৽Ȣɣɦɥă౼౿HEN1ɉ2ʤʇ˄ʂʙʡယɈ ʂĜʨĜʧˋʈɬ૩Ⱦ21 - 24ʤʇ˄ʂʙʡɈ RNA 2୭੍ĂmiRNA / miRNAȤɢɍsiRNA 2୭ ੍ɬູڽโɅʹʙ˃ݛȳɥăHEN1ɉ1Ⴈ੍RNA ȤɢɍDNAɉʹʙ˃ݛȱɄȞă
ᄻ ᅰćArabidopsisᄻᅰɈʇ˅ĜʣˋʈȱȹHEN1 ۆ๙ાɬᄵȳɥ෬ࣳă
ཱܾ௸ॣć1X NEBuffer 2Į10 mM Tris-HClĂ
50 mM NaClĂ1 mM DTTĂ10 mM MgCl2ĪpH 7.9Ă25Ŏīį128 ̂M S-adenosylmethionine ɂ500 ng miRNA 2୭੍ɬ࠸ɚ25 ̂lɈཱܾܕĂ 37ŎɁɼˋʅʽʱĜʏʿˋă
༂Ʌɢɥߤ౯ݛć70ŎɁ10ယ࠰
ʹʺć25 ̂lɈཱܾܕෂɅȤȞɀ500 ngɈ miRNA 2୭੍ɘȹɉ75 pmolɈ3ŌჂɬࠊɅ ʹʙ˃ݛȳɥă
SAMɉ ᅙ ɅH2OɁ1ć10Ʌ ࡏ ଫ ȳ ɥă miRNAɬɺɼʕʠĜʯ࿈ૻȳɥɅɉĂʋĜ˃ʡ SAMɈൣɩɤɅ2 ̂lɈĮ14CįSAMɬᅙȳɥă ߤ౯ɬ௫ȬɥȹɛɅmiRNAɈᆲɬঌɣȳȭɂ ɜݢ༑ɁȜɥă
ʾ ʣ ʛ ʠ ฤ ࡹć1 unitɉĂ37ŎĂ10ယ ࠰ ɁĂ1
pmolɈʹʙ˃ࡊɬmiRNA 2୭੍Ʌ๖ھȳɥɈɅ ྼᅟɄਊ೧ᆲɂฤࡹȯɦɥă
༏ć50,000 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
■siRNAȤɢɍmiRNAɈ3ŌჂɈˁʱ ˂ˋʈ
RNA 5
Ō
Pyrophosphohydrolase
Ī
RppH
ī
#M0356S 200 units . . . .¥ 15,500 ತცćʨʇʞ˂ɺRNA 5ŌPyrophosphohydrolase
ĪRppHīɉĂRNAɈ5Ōજ˂ˋબȥɣʬ˅˂ˋબ ɬதࢦȱɀĂ5Ōێ˂ˋબRNAɅȳɥăRppHʗ ˋʩʇଋɉNudH / YgdPɂȱɀɜඪɣɦĂʐɺ ʟʦʏˋ5Ō-র˂ˋબɬADPɂATPɅယߠȳɥă
ᄻᅰćE.coliɈRppHۆ๙ાɬ૩Ⱦ෬ࣳă
ཱ ܾ ௸ ॣć1X NEBuffer 2Į10 mM Tris-HClĂ
50 mM NaClĂ1 mM DTTĂ10 mMMgCl2ĂĪpH 7.9Ă25ŎīįĂ37ŎɁɼˋʅʽʱĜʏʿˋă
മᄞć
10X NEBuffer 2
ʾ ʣ ʛ ʠ ฤ ࡹć1ʾ ʣ ʛ ʠ ɉ37ŎĂ30ယ ɁĂ1
̂gɈ300-mer RNA๖ଖદဘɬĂXRN-1Ɂယސ ݢ༑ɄRNAɅȳɥਊ೧ᆲɂฤࡹȯɦɥă
༏ć5,000 unit/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
■5Ōજ˂ˋબRNAɬ5Ōʺʦ˂ˋબRNA Ʌဿࠓ
■ˁɼʊĜʏʿˋɈȹɛɈ5Ōʺʦ˂ˋ
બRNAɈ
■RNAɈ5ŌჂɈސ
5
Ō
Deadenylase
#M0331S 1,000 units . . . .¥ 12,500 ತცćਊၚ5ŌDeadenylaseɉʪʑʙʐˋજᇦ৭
ഢĪHITīʭɹʷ˂ĜɈĂູɅHintʮˁˋʙɅമ ȳɥʗˋʩʇଋɁȜɥăɺʯˁʗʅʏˋɈਊၚ ʂĜʕ˅ʈɁȜɥăʪʠɺʯˁʗʅʏˋ௫Ʌ౺ Ȳ ȹ ဿ ڽ ɉ త ॄ ଊ ࠏ Ɂ Ȝ ɥataxia oculomoto rataxia 1ĪAOA1īɅ࠲ᅇȱɀȞɥȭɂȦඪɣɦ ɀȞɥăʪʠɺʯˁʗʅʏˋɉĂDNAɈ5ŌჂ ɢɤAMPɬதࢦȳɥȭɂĪAMP-DNAݡယސ ਊ೧ߤ౯īɅɢɤĂࠊɄˁɼʊĜʏʿˋෂ ࠰ൌɬސȳɥăɘȹĂ3Ō-˂ˋબĂ3Ō-ʳʑʳ ʈ˂ʋĜ˃બɬதࢦȳɥȭɂɅɢɤĂ3ŌჂɈ DNAഹɬୌဌȳɥăʪʠɺʯˁʗʅʏˋɉʤʇ ˄ʂʙʡʵ˂˂ˋબܶʐɺʟʦʏˋહ˂ˋબ ĪAppppAīĂlysyl-AMPɄɃɈฝယાɅߤ౯ɬȳă
5ŌDeadenylaseɉS. cerevisiaeɈHNT3ۆ ๙ ા ɅʋĜʡȯɦɀȞɥăNEBɉĂȭɈਊ೧ȦDNA ȤɢɍRNAɈ5ŌჂɬංɺʟʣ˃ݛȱĂ5Ō˂ˋ બݛჂɬદ౺ȳɥȭɂɬცȱɀȞɥăɘȹĂ ȭɈਊ೧ɉAppppAɬATPȤɢɍAMPɅಜඣȳ ɥălysyl-AMPɅൎȳɥߤ౯ɉॳȯɦɄȞă
ᄻᅰćS. cerevisiaeᄻᅰɈ5ŌDeadenylaseɬ ʋĜʡȳɥʯˁʑʷʡɬᄵȳɥ෬ࣳɢɤ౼౿ă
ཱܾ௸ॣć1X NEBuffer 2Į50 mM NaClĂ10 mM
Tris-HClĂ10 mM MgCl2Ă1 mM DTTĪpH 7.9Ă 25ŎīįĂ5 - 50 pmolɺʟʣ˃ݛDNA ĪAMP-DNAī ɬ࠸ɚ20 ̂lཱܾܕĂ30ŎɁɼˋʅʽʱĜʏʿˋă ༂Ʌɢɥߤ౯ݛć70ŎɁ20ယ࠰
ʾʣʛʠฤࡹć1 unitɉĂ30ŎĂ10ယ࠰ɁĂ10
pmolɈAMPɬ5Ō-ɺʟʣ˃ݛDNAʂ˂ʌȥɣಜ ɤᆋȳɈɅྼᅟɄਊ೧ᆲɂฤࡹȯɦɥă
༏ć20,000 units/ml
ઞ৽ဦॷćwww.nebj.jpɬઞ
■DNAȤɢɍRNAɈ5ŌჂɈංɺʟʣ ˃ݛ
■ɺʯˁʗʅʏˋگശ౯DNAୌဌɺʛ
ʓɼ
■ʐʤʇ˄ʂʙʡહ˂ˋબɈސ
RN
A研究用試薬
RNase I
f
#M0243S 5,000 units . . . .¥ 11,000 #M0243L 25,000 units . . . .¥ 44,000
ತცć˂ʴʤʇ˄ɺĜʔIfĪRNase Ifīɉ1Ⴈ੍ ູڽโRNAʀˋʡʤʇ˄ɺĜʔɁȜɤĂȳɓɀ ɈRNAʐʤʇ˄ʂʙʡय़ਗɬސᇜȱĂ5Ōʪʡ˅ ʅʏɂ2Ō, 3Ōʍɼʇ˂ʛʇێ˂ˋબɬ౺Ȳɥă RNase If ɉĂRNase IĪE. coliɅᄻᅰīȤɢɍʶ ˃ʠĜʑय़ਗʗˋʩʇଋɈ˂ʋˋʫʢˋʠᅃਗ ʗˋʩʇଋɁȜɤĂRNase IɂວێɈߤ౯ɬᄵȳɥă
ᄻᅰć෬ࣳᄻᅰɈRNase Iۆ๙ાĪrnaīɂʶ ˃ʠĜʑय़ਗʗˋʩʇଋĪMBPīɬʋĜʡȳɥ ۆ๙ાɈᅃਗۆ๙ાɬᄵȳɥ෬ࣳă
ཱ ܾ ௸ ॣć1X NEBuffer 3Į100mM NaClĂ 50
mM Tris-HClĂ10 mM MgCl2Ă1 mM DTTĪpH 7.9Ă25ŎīįĂ37ŎɁɼˋʅʽʱĜʏʿˋă ༂Ʌɢɥߤ౯ݛć70ŎɁ20ယ࠰
ᅙ௫ɈڷćRNase IfɉDNAɬಜඣȱɄȞă
RNase If ɉĂ2Ⴈ੍RNAɅɓɀ1Ⴈ੍RNAɅൎ ȳɥߤ౯ȦȞă
࿚ଋࠣᆅćʀʅʕʤʇ˄ɺĜʔɂʀˋʡʤʇ˄
ɺĜʔɈʋˋʗʷʥĜʏʿˋȦჟȞȭɂɬߊă
ʾʣʛʠฤࡹć1 unitɉĂ1X NEBuffer 3ɬ࠸ɚ
10 ̂lɈཱܾᅗܕෂɁĂ15ယ࠰ɁĂ1 pmolɈਗ 1Ⴈ੍RNA 33-merɬࠊɅಜඣȳɥɈɅྼᅟ Ʉ ਊ ೧ ᆲ ɂ ȱ ɀ ฤ ࡹ ȯ ɦ ɥă ȭ Ɉ ཱ ܾ ɉĂ SYBR® GoldɁಹఋȱȹ20%ɺʇ˂˃ɺʷʡʊ ˃Ī40ć1 BisīɁߊăʾʣʛʠɺʛʓɼɈଌ অ௸ॣɉwww.nebj.jpɬઞă
༏ć50,000 units/ml
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■˂ʋˋʫʢˋʠɈᄻᅰȥɣඍᆋ
■DNAȤɢɍʗˋʩʇଋᆧȥɣRNA ɬதࢦ
■1Ⴈ੍RNAɬʺʦʤʇ˄ʂʙʡĂʐʤ ʇ˄ʂʙʡĂʠ˂ʤʇ˄ʂʙʡɅယސ
■˂ʴʤʇ˄ɺĜʔʯ˅ʞʇʏʿˋɺʛ
ʓɼ
RNase H
#M0297S 250 units . . . .¥ 12,000 #M0297L 1,250 units . . . .¥ 48,000
ತცć˂ʴʤʇ˄ɺĜʔHĪRNase HīɉĂDNA Ʌʧɼʮ˂ʘɼʔĜʏʿˋȱȹRNAɈʳʑʳʐ ʀʑʞ˃य़ਗɬູڽโɅݡယސȳɥʀˋʡ˂ ʴʤʇ˄ɺĜʔɁȜɥăȭɈਊ೧ɉ1Ⴈ੍DNAɘ ȹɉ2Ⴈ੍DNAɬಜඣȱɄȞă
ᄻ ᅰćEscherichia coliȥ ɣ ʇ ˅ Ĝ ʣ ˋ ʈ ȱ ȹ RNase Hۆ๙ાĪrnhīɬᄵȳɥ෬ࣳă
ཱܾ௸ॣć1X RNase H Reaction BufferĮ75
mM KClĂ50 mM Tris-HClĪpH 8.3Ă25ŎīĂ3 mM MgCl2Ă10 mM DTTįĂ37ŎɁɼˋʅʽʱĜ ʏʿˋă
༂Ʌɢɥߤ౯ݛć65ŎɁ20ယ࠰
࿚ଋࠣᆅćʀʅʕʤʇ˄ɺĜʔɂʀˋʡʤʇ˄
ɺĜʔɈʋˋʗʷʥĜʏʿˋȦჟȞȭɂɬߊă
ʾʣʛʠฤࡹć1 unitɉĂ50 ̂lɈཱܾᅗܕෂɁĂ
37ŎĂ20ယ࠰ɁĂĮ3Hį-࿈ૻpolyĪrAī
sPOLYĪdTī ɈRNA 1 nmolɬબݢᅗ౯˂ʴʤʇ˄ʂʙʡɅݡ ယސȳɥɈɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦ ɥăʾʣʛʠɺʛʓɼɈଌঅ௸ॣɉwww.nebj.jp ɬઞă
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■˂ʋˋʫʢˋʠɈᄻᅰȥɣඍᆋ
■polyĪdTīɅʧɼʮ˂ʘɼʔĜʏʿˋ ȱȹmRNAɈpolyĪAīʞĜ˃ɬதࢦ
■cDNA൦੍ਗ૪ɈmRNAɈதࢦ
RNase HII
#M0288S 250 units . . . .¥ 10,500 #M0288L 1,250 units . . .¥ 42,000
ತცćRibonuclease HIIĪRNase HIIīɉDNA 2 ୭੍Ɉ˂ʴʤʇ˄ʂʙʡɈ5ŌതɅᄭಪโɅʣʛ ʇɬɦɥʀˋʡ˂ʴʤʇ˄ɺĜʔɁȜɥăȭ Ɉਊ೧ɉ5Ō˂ˋબȤɢɍ3Ōʪʡ˅ʅʏჂɬદ ౺ȳɥăɘȹĂȭɈਊ೧ɉ݊ʭˁʈʹˋʠɈ RNA੍തɅထౘɈʣʛʇɬɦɥă
ᄻᅰćE. coliᄻᅰɈRNase HIIĪrnhBīۆ๙ાɂ ʶ˃ʠĜʑय़ਗʗˋʩʇଋĪMBPīɬʋĜʡȳ ɥۆ๙ાɈᅃਗۆ๙ાɬᄵȳɥ෬ࣳăɺʭɻ ʣʞɻĜʇ˅ʶʠʈˁʭɻĜɁ౼౿ষĂFactor XaɅɢɤMBPɬRNase HIIȥɣಜɤᆋȱĂȯɣ ɅMBPȤɢɍFactor XaɬதࢦȳɥăMBPȦಜ ɤᆋȯɦȹRNase HIIɈNჂɅɉ4ɺʷʦબર ࡊĪIle-Ser-Glu-PheīȦݡȯɦɀȞɥă
ཱܾ௸ॣć1X ThermoPol Reaction BufferĮ10
mM KClĂ20 mM Tris-HClĂ10 mMĪNH4ī2 SO4Ă2 mM MgSO4Ă0.1% Triton X-100ĪpH 8.8Ă 25ŎīįĂ37ŎɁɼˋʅʽʱĜʏʿˋă
࿚ଋࠣᆅćʀʅʕʤʇ˄ɺĜʔɂʀˋʡʤʇ˄
ɺĜʔɈʋˋʗʷʥĜʏʿˋȦჟȞȭɂɬߊă
ʾʣʛʠฤࡹć1 unitɉĂ1X ThermoPol Reaction
BufferෂɁĂ37ŎĂ30ယ࠰ɁĂ100 pmolɈਗ 2Ⴈ੍DNAࡊଋɅʣʛʇɬȱĂȷɦɅഊຎȳ ɥॊʏʈʢ˃ɬཡȳɥɈɅྼᅟɄਊ೧ᆲɂฤࡹ ȯɦɥă2Ⴈ੍DNAࡊଋɉॊఋ೧ယા / ʇʀˋ ʙʻĜʲɺɈʇʀˋʙʻĜࣷɅ˂ʴʤʇ˄ʂ ʙʡɬ1ܶࡊ࠸ɭɁȞɥăʾʣʛʠɺʛʓɼɈଌ অ௸ॣɉwww.nebj.jpɬઞă
༏ć5,000 units/ml
ʹʺć0.1% SDSɬ๒ݡȱɀɼˋʅʽʱĜʏʿ ˋȳɦɊĂRNase HIIɬယɅߤ౯ݛȳɥȭɂ ȦɁȧɥă
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ˁɼʓˋʑ/ູࢮ/ჲಗૢਐć379 - 382ʲĜʐɬઞ
■ʵ˂ʹˁĜʔદဘɈ˂ʴʤʇ˄ʂʙʡ
ଲɤਮəငڮɬʣʛʅˋʈ
■T7 Endo IɂࢼɅᅙȱ˂ʴʤʇ˄ʂ ʙʡଲਮəငڮɁ2Ⴈ੍ɬಜඣ
■݊ʭˁʈʹˋʠĂRNA / DNAʧɼ ʮ˂ʛʡɈRNA੍Ɉယސ
Antarctic Phosphatase
#M0289S 1,000 units . . . .¥ 10,000
#M0289L 5,000 units . . . .¥ 40,000 ੧ɉ123ʲĜʐɬઞ
Alkaline Phosphatase Calf Intestinal
Ī
CIP
ī
#M0290S 1,000 units . . . .¥ 10,000#M0290L 5,000 units . . . .¥ 40,000 ੧ɉ123ʲĜʐɬઞ
T4 Polynucleotide Kinase
#M0201S 500 units . . . .¥ 10,500
#M0201L 2,500 units . . . .¥ 42,000 ੧ɉ122ʲĜʐɬઞ
