Exp 34 36 8 Recent site activity Bac_Pathog

(1)

Mycology lab and negative staining

Chapter 34-36, 8

江倪全

https://sites.google.com/a/gap.cgu.edu.tw/bac_pathog/lecture

醫學⼆ (2018/05/03)、中醫⼆ (2018/05/09)

References

(2)

Morphology of molds and yeasts

✓

_Mold

-

Septate hyphae

-

Coenocytic

hyphae

✓

_Yeast

-

Budding yeast

-

Fission yeast

✓

_{Dimorphic fungi}

- 37ºC: yeastlike

(3)

✓

_Phylum

-

Ascomycota (⼦囊菌⾨)

(Ascomycetes)

-

Basidiomycota (擔⼦菌⾨)

-

Glomeromycota (球囊菌⾨)

(Mucormycetes)

http://en.wikipedia.org

Ascospore

(⼦囊孢⼦)

Zygospore

(接合孢⼦)

Basidiospore

(4)

(5)

Classification of Fungi

⼦囊菌⾨

球囊菌⾨

分⽣孢⼦

孢⼦囊孢⼦

隔膜菌絲

無隔膜菌絲

Sexual spore

Asexual spore

Ascomycota

Glomeromycota

Conidiospore

(6)

Cultivation of molds and yeasts

✓

_{Culture conditions}

-

Brain heart infusion (BHI) agar; Sabouraud dextrose agar

✓ _{dextrose, peptone, agar, pH 5.6}

-

Temperature: 25ºC to 30ºC (Candida spp: 35ºC-37ºC)

-

Time: 2 weeks; fungi isolated from blood samples: 5-7 days

玻片培養法

- 耗時

- 觀察完整構造

膠帶法

- 快速

(7)

Cultivation of molds and yeasts

C. albicans

(green)

C. tropicalis

(blue)

Mold

Yeast

http://thunderhouse4-yuri.blogspot.ca

(8)

Septum

(9)

Morphology of yeast

http://www.studyblue.com

(10)

Yeast morphology

Saccharomyces

cerevisiae

Candida

albicans

Yeast cell

(11)

Exp-10. Negative staining

Without staining

Positive staining

(12)

Negative staining for

Cryptococcus

neoformans

Yeast cell

(13)

Identification of unknown fungi

Lactophenol cotton blue

Conidiospore

Sporangiospore

http://thunderhouse4-yuri.blogspot.ca

Penicillium

(14)

Identification of unknown fungi

Conidiospore

Sporangiospore

Alternaria

http://mycota-crcc.mnhn.fr

(15)

(16)

Identification of unknown fungi

Conidiospore