201314年カタログ New England Biolabs Japan

(1)

౫ঔਊ೧Ɉ௢੧ɉʞʇʣʃ˃˂ʭɹ˄ˋʑɬ Ȯઞ௎ȩȺȯȞă

■_{౫ঔਊ೧ߤ౯ʙʻĜʠ}

■_{౫ঔਊ೧ཱܾɈ੕ไݛ}

■_{ʠˁʮ˃ʏʽĜʞɻˋʈʄɼʡ}

■_{ɼʕʏʖʶĜ˂ʑʠ}

■_Time-Saver_{࿚ଋਊ೧˂ʑʠ}

■_{ʹʙ˃ݛࠐି౯˂ʑʠ}

ʂ ˋ ˁ ɼ ˋ ʜ Ĝ ˃ ɞ ౫ ঔ ਊ ೧ Ɉ ʙʽĜʠ˂ɺ˃຦ކĪܑাīɬ༯ఒෂ

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ʘĜɂȱɀߤᄠȱɀȞɘȳă਱ষɜȭɈߤᄠɬڿ૩ȳɥȹɛɅĂNEBɉ੕਒஋๱ɂ྘ᇆɄȧ࿚ଋɬ

ᄵȳɥਊ೧ɈާཡɅଲɤ೨ɭɁȞɘȳă

NEBɈݨߔଞɉࡕശɈ౿࿚ɬޜᆯȱറȫɀȞɥȺȫɁɄȩĂఛȱȞࡳ୹ɅȤȫɥଌᅙݛɜඑઅȱ

ɀȞɘȳăȷɈय़ݪĂౘęɈݨߔሄဦɬཡ࿍ȱȹɤॸ࢞ྣɬ߉ຶȱȹɤȱɀȞɘȳăູɅ౫ঔਊ೧ Ɉॸ࢞ɂާཡ౿ഢɅᆵɬ෉ȞɁȤɤĂ௵Ʌ˂ĜʘĜʏʛʯɬଲȽɀȞɥɂ૵࿹ȱɀȞɘȳăȭɦɉ ˂ʋˋʫʢˋʠਊ೧ɬ஑ɛɀྀ༸ȱȹȭɂĂʣʛʅˋʈਊ೧ɬ஑ɛɀާཡȱȹȭɂĂȷɦɅʶʑ ʗĜʷʛʇʑ౫ঔਊ೧ɬಪऄȫɀอࢷȱɀȞɥȭɂɅᆈ࿥ȫɣɦɘȳăݡȢɀ౫ঔਊ೧ɬۆ๙ાޜ ဿȳɥȭɂɅɢɥູڽ౯ɂ౯༑Ɉ৐௫ɂȞȠߓఛโࡳ୹ɬභȞɀȞɘȳăȭȠȱȹॸ࢞ɬॅറȳɥ ȭɂɅɢɤĂ੕਒Ɉ౯༑ɂᆀ၆౯ɬอࢷȳɥȭɂɬȤᄝഩȱɘȳă

ɺɼʋˋɈತც

NEBɁʇ˅Ĝʣˋʈȱȹਊ೧

˂ʋˋʫʢˋʠਊ೧

ʧɼʭɻʟ˂ʞɻĜĪHFī౫ঔਊ೧ć૫౦ൣळʧɼʩʭʁĜʶˋʑ

౫ঔਊ೧

RE-MixĪʶʑʗĜʷʛʇʑ౫ঔਊ೧ī

Time-Saver࿚ଋਊ೧ć1̂lɈਊ೧ɬᅙȞɀైயཱܾ௸ॣݚɁ1̂g

ɈDNAɬ5 - 15ယ࠰ɁಜඣɁȧĂʂĜʨĜʢɼʠཱܾɬ਄Ƚɀɜʍˋ

ʯ˃ɉယސȯɦɄȞȭɂɬߊ໻੟əă௢੧ɉ287 - 288ʲĜʐɬઞ௎ă

S-adenosylmethyonineĪSAMīȦ်ʨɼɺ˃Ɂ࿥മă100%Ɉߤ

౯ɬຶɥȹɛɅĂ౿࿚ʟĜʗʃĜʡɅࡥ੪ȯɦȹ୒༏๱Ʌ୦ȽɀĂ

SAMɬ1X਼ཱܾਗဘɅ๒ݡăSAMȦྼీɄਊ೧ɅɈəɅ࿥മ

damĂdcmĂCpGʹʙ˃ݛࠐି౯ĪCpGʹʙ˃ݛɉణ߇౺ဘɈʊ

ʦʸDNAൎ௣īă௢੧ɉ315 - 317ʲĜʐɬઞ௎ă

ਊ೧Ɉ૖ไཱܾݗ๱

༂Ʌɢɥ࿤ߤ౯ݛɈݢྏă65ŎɁ20ယ࠰ɼˋʅʽʱĜʏʿˋȳɥ

ȭɂɁ࿤ߤ౯ݛ૚অɬ਄ȞĂ࿤ߤ౯ݛȯɦɄȥȽȹ௲ਗĂ80ŎɁ

20ယ࠰ɼˋʅʽʱĜʏʿˋȳɥȭɂɁ࿎ݟɬ਄Ƞă࿤ߤ౯ݛݗ๱ɉ

ɺɼʋˋෂɅ࿍૲ă

(2)

1

౫ঔਊ೧

AatII AccI Acc65I AciI AclI AcuI AfeI AflII AﬂIII AgeI AgeI-HF AgeI-HF RE-Mix AhdI AleI AluI AlwI AlwNI ApaI ApaLI ApeKI ApoI AscI

AscI RE-Mix AseI AsiSI AvaI AvaII AvrII BaeI BaeGI BamHI BamHI-HF BanI BanII BbsI BbvI BbvCI BccI BceAI BcgI BciVI BclI BcoDI BfaI BfuAI BfuCI BglI BglII BlpI BmgBI BmrI BmtI BmtI-HF BpmI Bpu10I BpuEI BsaI BsaI-HF BsaAI BsaBI BsaHI BsaJI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BslI BsmI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspCNI BspDI BspEI BspHI BspMI BspQI BsrI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI BstAPI BstBI BstEII BstEII-HF

BstEII-HF RE-Mix BstNI BstUI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI BtsIMutI BtsCI Cac8I ClaI CspCI CviAII CviKl-1 CviQ1 DdeI DpnI DpnII DraI DraIII-HF DrdI EaeI EagI EagI-HF EarI EciI Eco53kI EcoNI EcoO109I EcoP15I EcoRI EcoRI-HF

EcoRI-HF RE-Mix EcoRV EcoRV-HF EcoRV-HF RE-Mix FatI FauI Fnu4HI FokI FseI FspI HaeII HaeIII HgaI HhaI HincII HindIII HindIII-HF HinfI HinP1I HpaI HpaII HphI Hpy99I Hpy166II Hpy188I Hpy188III HpyAV HpyCH4III HpyCH4IV HpyCH4V KasI KpnI KpnI-HF

KpnI-HF RE-Mix MboI

MboII

MfeI MfeI-HF

MfeI-HF RE-Mix MluI MluCI MlyI MmeI MnlI MscI MseI MslI MspI MspA1I MwoI NaeI NarI NciI NcoI NcoI-HF NcoI-HF RE-Mix NdeI NgoMIV NheI NheI-HF NheI-HF RE-Mix NlaIII NlaIV NmeAIII NotI NotI-HF NotI-HF RE-Mix NruI NsiI NspI PacI PacI RE-Mix PaeR7I PciI PflFI PﬂMI PhoI PleI PluTI PmeI PmlI PpuMI PshAI PsiI PspGI PspOMI PspXI PstI PstI-HF PvuI PvuI-HF PvuII PvuII-HF RsaI RsrII SacI SacI-HF SacII SalI SalI-HF SalI-HF RE-Mix SapI Sau3AI Sau96I SbfI SbfI-HF ScaI-HF ScaI-HF RE-Mix ScrFI SexAI ʧɼʭɻʟ˂ʞɻĜ౫ঔਊ೧

AgeI-HF, BamHI-HF, BmtI-HF, BsaI-HF, BstEII-HF, DraIII-HF, EagI-HF, EcoRI-HF, EcoRV-HF, HindIII-HF, KpnI-HF, MfeI-HF, NcoI-HF, NheI-HF, NotI-HF, PstI-HF, PvuI-HF, PvuII-HF, SacI-HF,

SalI-HF, SbfI-HF, ScaI-HF, SphI-HF, SspI-HF, StyI-HF 19, 20

RE-MixĪʶʑʗĜʷʛʇʑ౫ঔਊ೧ī

AgeI-HF RE-Mix, AscI RE-Mix, BstEII-HF RE-Mix, EcoRI-HF RE-Mix, EcoRV-HF RE-Mix, KpnI-HF RE-Mix, MfeI-HF RE-Mix, NcoI-HF RE-Mix, NheI-HF RE-Mix, NotI-HF RE-Mix, PacI RE-Mix, SalI-HF RE-Mix,

ScaI-HF RE-Mix, SpeI RE-Mix, XbaI RE-Mix, XhoI RE-Mix 18, 20

ʹʙ˃ݛࠐି౯౫ঔਊ೧Īʀʬʐɿʥʞɻʇʑॸ࢞ᅙਊ೧ī

AbaSI, DpnI, DpnII, FspEI, HpaII, LpnPI, McrBC,

MspI, MspJI 244 - 245

ʣʛʅˋʈʀˋʡʤʇ˄ɺĜʔ

Nb.BbvCI, Nb.BsmI, Nb.BsrDI, Nb.BtsI, Nt.AlwI,

Nt.BbvCI, Nt.BsmAI, Nt.BspQI, Nt.BstNBI, Nt.CviPII 71 - 72

ʳĜʷˋʈʀˋʡʤʇ˄ɺĜʔ

I-CeuI, I-SceI, PI-PspI, PI-SceI 73 - 74

NEBuffers, Diluents, Gel Loading Dyes 75 SfaNI SfcI SﬁI SfoI SgrAI SmaI SmlI SnaBI SpeI SpeI Re-Mix SphI SphI-HF SspI SspI-HF StuI StyI StyI-HF StyD4I SwaI Taqα I TﬁI TseI Tsp45I TspMl TspRI Tth111I XbaI XbaI RE-Mix XcmI XhoI

XhoI RE-Mix

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233 ହᇆɈ౫ঔਊ೧ɂʘʮ˃ʘɼʐɿʑʠȦݢ༑Ɂȳă

ైயཱܾʯ˅ʠʋĜ˃Ʌ୦ȽɀEcoRV-HF

RE-MixɁpXba DNAɬಜඣȱȹă˄ĜˋL

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#N3270īă5 - 15ယ࠰Ă1૪࠰ĂʂĜʨĜʢ

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R

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(4)

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ȹȺȫɘȳă

1 3 5 10 M 1 3 5 10 μl NEB EcoRI-HF

Competitor FastDigest®_EcoRI

Unwanted Cleavage

EcoRI-HF (NEB #R3101) ɉ਒༏๱Ɉਊ೧ɬ઴ᅙȱɀ ʂĜʨĜʢɼʠཱܾɬ਄Ƚ ɀ ɜ ʑ ʗ Ĝ ߤ ౯ ɬ ૲ ȯ Ʉ

Ȟă50 ̂LɈཱܾृɅȤȞ

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ɀĂ1 ̂gɈLambda DNA

ɬ ಜ ඣ ȱ ȹă37Ŏ Ɂ ʂ Ĝ

ʨĜʢɼʠɈɼˋʅʽʱĜ

ʏʿˋɬ਄ȽȹăMɉ1 kb

DNA Ladderɬ ૲ ȳĪNEB

#N3232īă

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Ȧਊ೧ࡳ୹ɅȤȫɥ˂ĜʘĜɁȜɥɂȞȠშ౾ɉ౫ঔਊ೧Ɉ࿚ଋɂఒᅲ౯

Ʌᆈ࿥ȫȯɦɀȞɘȳăȳɓɀɈ౫ঔਊ೧ɅൎȱɀউდɄ࿚ଋࠣᆅȦ਄ɩɦɀ

ȤɤĂ੕਒Ɉ஋๱ɂ˅ʛʠ࠰ɈিੇȦ੕ழঔɈਊ೧ɬอࢷȱɀȞɘȳă

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ȦჟȞȭɂɬߊ໻ȱɀȞɥă

DNAʋˋʗʷʥĜʏʿˋ

ਊ೧ᅗܕɬqPCRɅࢷȱĂʊʦʸDNAȦ਼໴ȱɀȞɄȞȭɂɬߊ

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ࡤ߆ɂȳɥă

ʀʅʕʤʇ˄ɺĜʔߤ౯

RI࿈ૻȱȹDNAɂ੕ఛૺɈʅʻʬˁ˂Ĝ๝࡜܋຦ɅࡊȿȞȹɺʛ

ʓɼၫၭɅɢɤĂ࣡ɛɀฝȞ˄ʱ˃Ɉʀʅʕʤʇ˄ɺĜʔߤ౯ɜ ॳ୸Ɂȧɥă

ʀˋʡʤʇ˄ɺĜʔߤ౯

ʣʛʅˋʈɞྥູڽโಜඣɬۜȧࡧȭȳਊ೧ȦᐍઔȱɀȞɄȞȭ

ɂɬߊ໻ȳɥȹɛĂ౫ঔਊ೧ɂʑĜʩĜʋɼ˃ʯˁʑʷʡDNAɬ

4૪࠰ɼˋʅʽʱĜʏʿˋȱĂʀˋʡʤʇ˄ɺĜʔߤ౯ȦჟȞȭɂ

ɬߊ໻ȳɥă

ྥູڽโDNaseߤ౯ ౫ঔਊ೧ɂLambda DNAɬʂĜʨĜʢɼʠɁɼˋʅʽʱĜʏʿˋ

ȱĂྥູڽโʤʇ˄ɺĜʔߤ౯ȦჟȞȭɂɬߊ໻ȳɥă

ʇ˅Ĝʣˋʈ૚অ ĪˁɼʊĜʏʿˋȤɢɍ ˂ʃʛʠī

߂౫ঔਊ೧ɈࡊଋDNAɬݿ௰ಜඣȱĂ˂ˁɼʊĜʏʿˋৌᆎȤɢ

(5)

RE-Mix

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_HF

ī౫ঔਊ೧

RE-Mixɂʧɼʭɻʟ˂ʞɻĜĪHFī౫ঔਊ೧NEBɉᄘ౺ळ౫ঔਊ

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RE-Mix

ʶʑʗĜʷʛʇʑ

Product Recognition Site NEB #

AgeI-HF RE-Mix

▼

▲ 5´. . . A C C G G T. . .3´

3´. . . _{T G G C C A}. . .₅´ R5552S

AscI RE-Mix

5₃´_´. . ._{. . .} G G C G C G C C _{C C G C G C G G}. . ._{. . .}3₅´_´ ▼

▲

R5558S

BstEII-HF RE-Mix

5₃´_´. . .. . . G G T N A C C _{C C A N T G G}. . .. . .3₅´´

▼ ▲ R5162S

EcoRI-HF RE-Mix

▼ ▲ 5´. . .G A A T T C. . . 3´

3´. . . C T T A A G. . . 5´ R5101S

EcoRV-HF RE-Mix

5₃´_´. . .. . .G A T A T C_{C T A T A G}. . .. . .3₅´´

▼

▲

R5195S

KpnI-HF RE-Mix

5₃´_´. . ._{. . .}G G T A C C_{C C A T G G}. . ._{. . .}3₅´_´ ▼ ▲ R5142S

MfeI-HF RE-Mix

▼ ▲ 5´. . . _{C A A T T G}. . .₃´

3´. . .G T T A A C. . .5´ R5589S

NcoI-HF RE-Mix

▼

▲ 5´. . . _{C C A T G G}. . .₃´

3´. . .G G T A C C. . .5´ R5193S

NheI-HF RE-Mix

▼

▲ 5´. . .G C T A G C. . .3´

3´. . . _{C G A T C G}. . .₅´ R5131S

NotI-HF RE-Mix

5₃´_´. . ._{. . .}G C G G C C G C_{C G C C G G C G}. . ._{. . .}3₅´_´ ▲ ▼

R5189S

PacI RE-Mix

5₃´_´. . ._{. . .} T T A A T T A A _{A A T T A A T T}. . ._{. . .}3₅´_´ ▼

▲

R5547S

SalI-HF RE-Mix

▲ 5´. . .G T C G A C. . .3´

3´. . . _{C A G C T G}. . .₅´

▼

R5138S

ScaI-HF RE-Mix

5´

. . . _{A G T A C T}. . .₃´

3´. . . T C A T G A. . .5´

▼

▲

R5122S

SpeI RE-Mix

5₃´_´. . .. . . A C T A G T _{T G A T C A}. . .. . .3₅´´

▼ ▲ R5133S

XbaI RE-Mix

▼ ▲ 5´. . . T C T A G A . . .3´

3´. . ._{A G A T C T}. . .₅´ R5145S

XhoI RE-Mix

▼

▲ 5´. . . C T C G A G . . .3´

3´. . ._{G A G C T C}. . .₅´ R5146S

HF

౫ঔਊ೧

Product Recognition Site NEB #

AgeI-HF

▼

▲ 5´. . . A C C G G T. . .3´

3´. . . _{T G G C C A}. . .₅´ R3552S/L

BamHI-HF

▼

▲ 5´. . ._{G G A T C C}. . .₃´

3´. . . C C T A G G. . .5´ R3136S/L/T/M

BmtI-HF

5₃´´. . .. . . G C T A G C _{C G A T C G}. . .. . .3₅´´

▲ ▼

R3658S/L

BsaI-HF

5

´. . ._{G G T C T C (N)} 1 . . .3´ 3´. . . C C A G A G (N)₅ . . .5´

▼

▲

R3535S/L

BstEII-HF

5₃´_´. . ._{. . .} G G T N A C C _{C C A N T G G}. . ._{. . .}3₅´_´ ▼

▲

R3162S/L

DraIII-HF

5₃´_´. . ._{. . .}C A C N N N G T G_{G T G N N N C A C}. . ._{. . .}3₅´_´ ▲ ▼ R3510S/L

EagI-HF

▼ ▲ 5´. . . _{C G G C C G}. . .₃´

3´. . .G C C G G C. . .5´ R3505S/L/M

EcoRI-HF

▼

▲ 5´. . .G A A T T C. . . 3´

3´. . . C T T A A G. . . 5´ R3101S/L/T/M

EcoRV-HF

5₃´_´. . .. . .G A T A T CC T A T A G. . .. . .35´´

▼ ▲ R3195S/L/T/M

HindIII-HF

▼ ▲ 5´. . . A A G C T T . . .3´

3´. . ._{T T C G A A}. . .₅´ R3104S/L

KpnI-HF

5₃´_´. . ._{. . .}G G T A C C_{C C A T G G}. . ._{. . .}3₅´_´ ▼ ▲ R3142S/L/M

MfeI-HF

▼ ▲ 5´. . . _{C A A T T G}. . .₃´

3´. . .G T T A A C. . .5´ R3589S/L

NcoI-HF

▼

▲ 5´. . . _{C C A T G G}. . .₃´

3´. . .G G T A C C. . .5´ R3193S/L/M

NheI-HF

▼

▲ 5´. . .G C T A G C. . .3´

3´. . . _{C G A T C G}. . .₅´ R3131S/L/M

NotI-HF

5₃´_´. . ._{. . .}G C G G C C G C_{C G C C G G C G}. . ._{. . .}3₅´_´ ▲ ▼

R3189S/L/M

PstI-HF

5₃´_´. . .. . ._{G A C G T C}C T G C A G. . .. . .3₅´´

▼

▲

R3140S/L/T/M

PvuI-HF

5₃´_´. . ._{. . .} C G A T C G _{G C T A G C}. . ._{. . .}3₅´_´ ▼

▲

R3150S/L

PvuII-HF

5´

. . . _{C A G C T G}. . .₃´

3´. . .G T C G A C. . .5´

▼

▲

R3151S/L/M

SacI-HF

5₃´_´. . .. . .G A G C T C_{C T C G A G}. . .. . .3₅´´

▼

▲

R3156S/L/M

SalI-HF

▲ 5´. . ._{G T C G A C}. . .₃´

3´. . . C A G C T G. . .5´

▼

R3138S/L/T/M

SbfI-HF

5₃´_´. . .. . ._{G G A C G T C C}C C T G C A G G. . .. . .3₅´´

▼

▲

R3642S/L

ScaI-HF

5₃´_´. . ._{. . .} A G T A C T_{T C A T G A}. . ._{. . .}3₅´_´ ▼

▲

R3122S/L/M

SphI-HF

5₃´_´. . .. . .G C A T G C_{C G T A C G}. . .. . .3₅´´

▼

▲

R3182S/L/M

SspI-HF

5₃´_´. . .. . . A A T A T T_{T T A T A A}. . .. . .3₅´´

▼

R3132S/L/M

Produ

制限酵素

(6)

AatII

NEBuffer 1.1 2.1 3.1 CutSmart

% Activity <10 50* 50 100

#R0117S 500 units . . . . .¥ 9,000 #R0117L 2,500 units . . . .¥ 36,000

ູෳćCutSmartĂ˂ʋˋʫʢˋʠĂTime-Saver

ཱܾ௸ॣćCutSmart BuferĂ37Ŏ

༂Ʌɢɥ࿤ߤ౯ݛć80ŎĂ20ယ࠰

༏๱ć20,000 units/ml

ʹʙ˃ݛࠐି౯ć፪໳ᇆʊʦʸDNAɈಜඣɉĂ

CpGʹʙ˃ݛɅɢȽɀʮ˅ʛʇȯɦɥĪp315ɬ

ઞ௎īă

ʹ ʺćཱ ܾ ʨ ʛ ʭ ɹ Ĝ ȦpH 7.5 - 8.0Ī25Ŏī

ɈཱྀڱެɁȜɥ௲ਗɉĂߤ౯Ȧฝݚȳɥă * NEBuffer 2.1ɬ઴ᅙȱȹ௲ਗĂʑʗĜߤ౯ɬ౺Ȳɥȭ

ɂȦȜɥă

5 ´

. . .

G A C G T C

. . .

3 ´

. . .

C T G C A G

. . .

5 ´

▼

▲

AbaSI

#R0665S 1,000 units ௢੧ɉ130ʲĜʐɬઞ௎

AccI

% Activity 50 50 10 100

#R0161S 1,000 units . . . .¥ 12,000 #R0161L 5,000 units . . . .¥ 48,000

ཱܾ௸ॣćCutSmart BuferĂ37Ŏ

CpGʹʙ˃ݛɂʂĜʨĜˁʛʯȳɥ௲ਗɅɉʮ

˅ʛʇȯɦɥĪp315ɬઞ௎īă

ʹʺćAcclȦ୥ယɅಜඣߤ౯ɬ૲ȳȹɛɅɉĂ

໻ૻ༯ᇙჂ඘ȥɣவɄȩɂɜ13ܶࡊൎȦྼᅟɁ

Ȝɥă

5 ´

. . .

G T M K A C

. . .

3 ´

. . .

C A K M T G

. . .

5 ´

▲

▼

Acc65I

NEBuffer 1.1 2.1 3.1 CutSmart

% Activity 10 75* 100 25

#R0599S 2,000 units . . . .¥ 11,000 #R0599L 10,000 units . . . .¥ 44,000

ູෳć˂ʋˋʫʢˋʠĂTime-Saver

ཱܾ௸ॣćNEBuffer 3.1Ă37Ŏ

ʹʙ˃ݛࠐି౯ćdcmʹʙ˃ݛɂʂĜʨĜˁʛ ʯȳɥ௲ਗɅɉʮ˅ʛʇȯɦɥă

፪ ໳ ᇆ ʊ ʦ ʸDNAɈ ಜ ඣ ɉĂCpGʹ ʙ ˃ ݛ ɂ

ʂ Ĝ ʨ Ĝ ˁ ʛ ʯ ȳ ɥ ௲ ਗ Ʌ ʮ ˅ ʛ ʇ ȯ ɦ ɥ

Īp315ɬઞ௎īă

ʹʺćAcc65IɉKpnlɈʥʂʏʖʶĜɁȜɥă

*ȭɈʨʛʭɹĜɬ઴ᅙȱȹ௲ਗĂʑʗĜߤ౯ɬ

౺ȲɥȭɂȦȜɥă

5 ´

. . .

G G T A C C

. . .

3 ´

. . .

C C A T G G

. . .

5 ´

▼

▲

AciI

% ActivityNEBuffer <101.1 2.125 1003.1 CutSmart100

#R0551S 200 units . . . .¥ 11,000 #R0551L 1,000 units . . . .¥ 44,000

ཱܾ௸ॣćCutSmart BufferĂ37Ŏ

ઞ௎īă

ʹʺćAciIɉྥʩ˂ˋʡ˅Ĝʸ໻ૻငڮɬᄵȳ

ɥă୦ȽɀĂAciIɁಜඣȱɀˁɼʊĜʏʿˋȱȹ

DNAɉ50%ȱȥAciIငڮɬ੔౺౰ȱɄȞă

5 ´

. . .

C C G C

. . .

3 ´

. . .

G G C G

. . .

5 ´

▼

▲

AclI

NEBuffer% Activity 1.1<10 <10 <102.1 3.1 CutSmart100

#R0598S 300 units . . . .¥ 11,000 #R0598L 1,500 units . . . .¥ 44,000

ઞ௎īă

ʹʺćAclIɉPspl406IɈʥʂʏʖʶĜɁȜɥă

5 ´

. . .

A A C G T T

. . .

3 ´

. . .

T T G C A A

. . .

5 ´

▼

▲

AcuI

% ActivityNEBuffer 1.150 1002.1 3.150 CutSmart100

#R0641S 300 units . . . .¥ 10,500 #R0641L 1,500 units . . . .¥ 42,000

ཱܾ௸ॣćCutSmart Buffer + SAMĂ37Ŏ

ʹʙ˃ݛࠐି౯ćdamĂdcmĂɘȹɉ፪໳ᇆɈ

CpGʹʙ˃ݛɅɢɥ܆ࣗɬିȫɄȞă

ʹʺćཱܾɅɉS-adenosylmethionineĪਊ೧Ʌ

࿥മīȦྼᅟɁȜɥă

5%ڬ௫Ɉʈ˂ʓ˅Ĝ˃༏๱ɁɉʑʗĜߤ౯ɬ૲

5 ´

. . .

C T G A A G (N)

₁₆

. . .

3 ´

. . .

G A C T T C (N)

₁₄

. . .

5 ´

▼

制限酵素

(7)

AfeI

% Activity 25 100 25 100

#R0652S 200 units . . . .¥ 11,000 #R0652L 1,000 units . . . .¥ 44,000

ູෳćCutSmartĂ˂ʋˋʫʢˋʠ

ઞ௎īă

ʹʺćAfeIɉEco47IIIɈɼʕʏʖʶĜɁȜɥă

5 ´

. . .

A G C G C T

. . .

3 ´

. . .

T C G C G A

. . .

5 ´

▼

▲

AﬂII

#R0520S 2,000 units . . . .¥ 10,000 #R0520L 10,000 units . . . .¥ 40,000

ʹ ʙ ˃ ݛ ࠐ ି ౯ćdamĂdcmĂ ɘ ȹ ɉ ፪ ໳ ᇆ

▼

▲

5 ´

. . .

C T T A A G

. . .

3 ´

. . .

G A A T T C

. . .

5 ´

AﬂIII

NEBuffer% Activity 1.110 2.150 1003.1 CutSmart50

#R0541S 250 units . . . .¥ 11,000 #R0541L 1,250 units . . . .¥ 44,000

ູෳć˂ʋˋʫʢˋʠ

5 ´

. . .

A C R Y G T

. . .

3 ´

. . .

T G Y R C A

. . .

5 ´

▼

▲

AgeI

NEBuffer 1.1 2.1 3.1 CutSmart

% Activity 100 75 25 75

#R0552S 300 units . . . .¥ 11,000 #R0552L 1,500 units . . . .¥ 44,000

ઞ௎īă

ෳ૪࠰ཱܾ૪ɅʑʗĜߤ౯ɬ૲ȳ௲ਗȦȜɥă

▼

▲

5 ´

. . .

A C C G G T

. . .

3 ´

. . .

T G G C C A

. . .

5 ´

AgeI-HF

% Activity 100 50 10 100

#R3552S 300 units . . . .¥ 11,000 #R3552L 1,500 units . . . .¥ 44,000

ູෳćCutSmartĂ˂ʋˋʫʢˋʠĂۆ๙ાޜဿ

ळĂTime-SaverĂʧɼʭɻʟ˂ʞɻĜ

ઞ௎īă

▼

▲

5 ´

. . .

A C C G G T

. . .

3 ´

. . .

T G G C C A

. . .

5 ´

AgeI-HF RE-Mix

#R5552S 25 reactions . . . . .¥ 5,400 ູෳćʶʑʗĜʷʛʇʑĂ˂ʋˋʫʢˋʠĂۆ

๙ાޜဿळĂTime-SaverĂʧɼʭɻʟ˂ʞɻĜ

ཱܾ௸ॣć20 ̂LɈཱܾᅗܕෂɅAgeI-HF RE-Mix

ɂDNAɬ๒ݡĂ37Ŏ

ઞ௎īă

▼

▲

5 ´

. . .

A C C G G T

. . .

3 ´

. . .

T G G C C A

. . .

5 ´

制限酵素

(8)

AhdI

% Activity 25 25 10 100

#R0584S 1,000 units . . . .¥ 12,000 #R0584L 5,000 units . . . .¥ 48,000

CpGʹʙ˃ݛɂʂĜʨĜˁʛʯȳɥ௲ਗɅɉ܆

ࣗɬିȫɥĪp315ɬઞ௎īă

ʹʺćAhdIɉEam1105IɈɼʕʏʖʶĜɁȜɥă

AhdlɉĂိߦჂ඘ඣ၀ɢɤˁɼʊĜʏʿˋȦਲ

໧Ʉ1ܶࡊ3Ōໃ୸Ⴢ඘ɬᄵȳɥDNAඣ၀ɬ౺౰

ȳɥă

5´. . .G A C N N N N N G T C. . .3´

3´. . .C T G N N N N N C A G. . .5´ ▼

▲

AleI

NEBuffer% Activity 1.1<10 <10 <102.1 3.1 CutSmart100

#R0634S 500 units . . . .¥ 11,000 #R0634L 2,500 units . . . .¥ 44,000

ʹʙ˃ݛࠐି౯ć፪໳ᇆʊʦʸDNAɈಜඣɅȤ

ȞɀĂCpGʹʙ˃ݛɂʂĜʨĜˁʛʯȳɥ௲ਗ

Ʌɉ܆ࣗɬିȫɥĪp315ɬઞ௎īă

ʹʺćAleIɉOliIɈɼʕʏʖʶĜɁȜɥă

30໲࠰ɬ෰Ȣɀ။ശȳɥ௲ਗĂ–70ŎɁ။ശȳɥă

5 ´

. . .

C A C N N N N G T G

. . .

3 ´

. . .

G T G N N N N C A C

. . .

5 ´

▼

▲

AluI

#R0137S 1,000 units . . . .¥ 10,000 #R0137L 5,000 units . . . .¥ 40,000

CpGɈʹʙ˃ݛɅɢɥ܆ࣗɬିȫɄȞă

5 ´

. . .

A G C T

. . .

3 ´

. . .

T C G A

. . .

5 ´

▼

▲

AlwI

% Activity 50 50 10 100

#R0513S 500 units . . . .¥ 11,000 #R0513L 2,500 units . . . .¥ 44,000

ʹ ʙ ˃ ݛ ࠐ ି ౯ćdamɈ ʹ ʙ ˃ ݛ Ʌ ɢ Ƚ ɀ ʮ

ʹʺćAlwIɉĂိߦჂ඘ඣ၀ɢɤˁɼʊĜʏʿˋ

Ȧਲ໧Ʉ1ܶࡊ5Ōໃ୸Ⴢ඘ɬᄵȳɥDNAඣ၀ɬ

౺౰ȳɥă

ཱܾ૪࠰ȦෳȞĂਊ೧༏๱Ȧ਒ȞĂʈ˂ʓ˅Ĝ

˃༏๱Ȧ> 5%Ɉ௲ਗĂʑʗĜߤ౯ɬ౺Ȳɥȭɂ

ȦȜɥă

5 ´

. . .

G G A T C (N)

₄

. . .

3 ´

. . .

C C T A G (N)

₅

. . .

5 ´

▼

▲

AlwNI

#R0514S 500 units . . . .¥ 11,000 #R0514L 2,500 units . . . .¥ 44,000

ʹʙ˃ݛࠐି౯ćʂĜʨĜˁʛʯȳɥdcmɈʹ

ʙ˃ݛɅɢȽɀʮ˅ʛʇȯɦɥĪp315ɬઞ௎īă

ฝ ɼ ʂ ˋ ࣅ ๱ĂpHȦ8ڬ ௫Ă ਊ ೧ ༏ ๱ Ȧ ਒ ȞĂ

ʈ˂ʓ˅Ĝ˃༏๱Ȧ> 5%Ɉ௲ਗĂʑʗĜߤ౯ɬ

౺ȲɥȭɂȦȜɥĪp284ɬઞ௎īă

5 ´

. . .

_{C A G N N N C T G}

. . .

₃

´

3 ´

. . .

_{G T C N N N G A C}

. . .

₅

´

▼

▲

ApaI

% Activity 25 25 <10 100

#R0114S 5,000 units . . . . .¥ 7,000 #R0114L 25,000 units . . . .¥ 28,000

37ŎɅȤȫɥߤ౯ć100%

Ⱥȹȱ37ŎɈ཰ঌࡖɉ30ယɁȜɥă

ʹʙ˃ݛࠐି౯ćdcmʹʙ˃ݛɂʂĜʨĜˁʛ ʯ ȳ ɥ ௲ ਗ Ʌ ʮ ˅ ʛ ʇ ȯ ɦ ɥă ፪ ໳ ᇆ ʊ ʦ ʸ

DNAɈಜඣɉĂCpGʹʙ˃ݛɂʂĜʨĜˁʛʯ

ȳɥ௲ਗɅʮ˅ʛʇȯɦɥĪp315ɬઞ௎īă

ʹʺćApaIɉBsp120IɈɼʕʏʖʶĜɁȜɥȦĂ

3Ōໃ୸Ⴢ඘ɬ౺౰ȳɥă

ApaIɉ50 ȉMڬ௫Ɉܶ༏๱Ɂ೫ޮȯɦɥă

5 ´

. . .

G G G C C C

. . .

3 ´

. . .

C C C G G G

. . .

5 ´

▼

▲

制限酵素

(9)

ApaLI

% Activity 100 100 10 100

#R0507S 2,500 units . . . .¥ 10,500 #R0507L 12,500 units . . . .¥ 42,000

਒༏๱࿚Ī5Xī

#R0507M 12,500 units . . . .¥ 42,000

༏๱ć10,000 units/mlȤɢɍ50,000 units/ml

ʹʺćApaLIɉM13 DNAɬಜඣȱɄȞȦĂȭɦ

ɉၡਠȯɦɀȞɥM13 DNAɈۆ๙ા༯ᇙȦ࠰

ۅȽɀȞɥȹɛɁȜɥă

౷ȱȞ༯ᇙɉGTGCTCɁȜɤĂȭɦɉBsiHKAI

ɘȹɉBsp1286IɈȞȴɦȥɁಜඣȳɥȭɂȦݢ

༑ɁȜɥă

▼

▲

5 ´

. . .

G T G C A C

. . .

3 ´

. . .

C A C G T G

. . .

5 ´

ApeKI

% Activity 25 50 100 10

#R0643S 250 units . . . .¥ 11,500 #R0643L 1,250 units . . . .¥ 46,000

༏๱ć5,000 units/m

ʹʺćApeKIɉTseIɈɼʕʏʖʶĜɁȜɥă

ApeKIɉྥ௵Ʌ਒Ȟ༂ڤฤ౯ɬ૲ȳ౫ঔਊ೧ɁȜ

ɤĂ95ŎɈ਒ݗɁɜߤ౯ɁȜɥă95ŎɈਊ೧Ɉ

཰ঌࡖɉ20ယɁȜɥă

5 ´

. . .

G C W G C

. . .

3 ´

. . .

C G W C G

. . .

5 ´

▼

▲

ApoI

% ActivityNEBuffer 101.1 2.175 1003.1 CutSmart75

#R0566S 1,000 units . . . . ¥ 11,500 #R0566L 5,000 units . . . .¥ 46,000

ʹʺćApoIɈಜඣɅɢɤ౺Ȳɥ5ŌAATTໃ୸Ⴢ඘

ɉĂEcoRIɅɢɤಜඣȱȹDNAඣ၀ɂˁɼʊĜ

ʏʿˋݢ༑ɁȜɥă

5 ´

. . .

R A A T T Y

. . .

3 ´

. . .

Y T T A A R

. . .

5 ´

▼

▲

AscI

% Activity <10 10 10 100

#R0558S 500 units . . . ._{¥ 11,000} #R0558L 2,500 units . . . ._{¥ 44,000}

ઞ௎īă

5 ´

. . .

G G C G C G C C

. . .

3 ´

. . .

C C G C G C G G

. . .

5 ´

▼

▲

AscI RE-Mix

#R5558S 50 reactions . . . .¥ 5,400 ູෳ ćʶʑʗĜʷʛʇʑĂ ˂ʋˋʫʢˋʠĂ

Time-Saver

ཱܾ௸ॣć20 ̂LɈཱܾᅗܕෂɅAscI-HF RE-Mix

ɂDNAɬ๒ݡĂ37Ŏ

ઞ௎īă

5 ´

. . .

G G C G C G C C

. . .

3 ´

. . .

C C G C G C G G

. . .

5 ´

▼

▲

AseI

% ActivityNEBuffer <101.1 2.150 1003.1 CutSmart10

#R0526S 2,000 units . . . .¥ 10,500 #R0526L 10,000 units . . . .¥ 42,000

਒༏๱࿚Ī5Xī

#R0526M 10,000 units . . . .¥ 42,000

ʹʺćAseIɉẮDNAɈಜඣɢɤ3༰ബȞബ๱Ɂ

pBR322ɂɺʟʦɾɼ˃ʑ-2ɈDNAɬಜඣȳɥă

౺ȲɥȭɂȦȜɥă

5 ´

. . .

A T T A A T

. . .

3 ´

. . .

T A A T T A

. . .

5 ´

▲

▼

制限酵素

(10)

AsiSI

% Activity 50 100 100 100

#R0630S 500 units . . . .¥ 11,000 #R0630L 2,500 units . . . .¥ 44,000

ઞ௎īă

ʹʺćAsiSIɉSgfIɈɼʕʏʖʶĜɁȜɥă

ཱܾ૪࠰ȦෳȞ௲ਗĂʑʗĜߤ౯ɬ౺Ȳɥȭɂ ȦȜɥă

5 ´

. . .

G C G A T C G C

. . .

3 ´

. . .

C G C T A G C G

. . .

5 ´

▼

▲

AvaI

% ActivityNEBuffer 1.1<10 1002.1 3.125 CutSmart100

#R0152S 2,000 units . . . .¥ 10,000 #R0152L 10,000 units . . . .¥ 40,000

਒༏๱࿚Ī5Xī

#R0152T 2,000 units . . . .¥ 10,000 #R0152M 10,000 units . . . .¥ 40,000

ઞ௎īă

ʹʺćAvaIɈ৕༂౯ɼʕʏʖʶĜɁȜɥBsoBIɜ

อࢷă

▼

5 ´

. . .

C Y C G R G

. . .

3 ´

. . .

G R G C Y C

. . .

5 ´

▲

AvaII

#R0153S 2,000 units . . . ._{¥ 10,000} #R0153L 10,000 units . . . ._{¥ 40,000}

਒༏๱࿚Ī5Xī

#R0153M 10,000 units . . . .¥ 40,000

ʹʙ˃ݛࠐି౯ćdcmʹʙ˃ݛɂʂĜʨĜˁʛ ʯȳɥ௲ਗɅɉʮ˅ʛʇȯɦɥă፪໳ᇆʊʦʸ

DNAɈಜඣɉĂCpGʹʙ˃ݛɂʂĜʨĜˁʛʯ

ȳɥ௲ਗɅɉʮ˅ʛʇȯɦɥĪp315ɬઞ௎īă

5 ´

. . .

G G W C C

. . .

3 ´

. . .

C C W G G

. . .

5 ´

▲ ▼

AvrII

% Activity 100 50 50 100

#R0174S 100 units . . . . .¥ 7,000 #R0174L 500 units . . . .¥ 28,000

ʹʺćAvrIIɈಜඣɅɢɤ౺Ȳɥ5ŌCTAGໃ୸Ⴢ

඘ɉĂNheIĂSpeIĂɘȹɉXbaIɈಜඣɅɢȽɀ

౺ȲɥDNAඣ၀ɂˁɼʊĜʏʿˋɁȧɥă

▼

▲

5 ´

. . .

C C T A G G

. . .

3 ´

. . .

G G A T C C

. . .

5 ´

BaeI

% Activity 50 100 50 100

#R0613S 250 units . . . ._{¥ 10,500} #R0613L 1,250 units . . . ._{¥ 42,000}

ູෳćCutSmartĂTime-Saver

ʹʺćBaeIɅɢɤDNAɬ2ޑಜඣȱɀ໻ૻငڮ

ɬಜɤ୸ȳȭɂɅɢɤĂ5ܶࡊෳɈ3ŌʂĜʨĜ

ʧˋʈɬᄵȳɥ28ܶࡊൎɈDNAඣ၀Ȧ౺Ȳɥă

ێငɈ໻ૻ༯ᇙɅȤȞɀɉ੆ూɈಜඣݳஒڬެ

ɅĂ1ܶࡊൎȴɦȹငڮɁಜඣȳɥȭɂȦၡਠȯ

ɦɀȞɥăਊ೧ཱܾɅɉS-adenosylmethionine

Īਊ೧Ʌ࿥മīȦྼᅟă

5´. .

10(N) A C (N)4 G T A Y C (N)12 . .3´

3´. .

15(N) T G (N)4 C A T R G(N)7 . ._▲5´ ▲

▼ ▼

制限酵素

(11)

BaeGI

NEBuffer 1.1 2.1 3.1 CutSmart % Activity 75 75 100 25

#R0708S 500 units . . . .¥ 10,500 #R0708L 2,500 units . . . .¥ 42,000

ູෳćTime-Saver

ʹʺćBaeGIɉBme1580IɈɼʕʏʖʶĜɁȜɥă

5 ´

. . .

G K G C M C

. . .

3 ´

. . .

C M C G K G

. . .

5 ´

▼

▲

BamHI

% Activity 75 100 100 100

#R0136S 10,000 units . . . . .¥ 6,000 #R0136L 50,000 units . . . .¥ 24,000

਒༏๱࿚Ī5Xī

#R0136T 10,000 units . . . . .¥ 6,000 #R0136M 50,000 units . . . .¥ 24,000

ʹʺćʈ˂ʓ˅Ĝ˃༏๱Ȧ> 5%Ɉ௲ਗĂʑʗĜ

ߤ౯ɬ౺ȲɥȭɂȦȜɥ

▼

▲

5 ´

. . .

G G A T C C

. . .

3 ´

. . .

C C T A G G

. . .

5 ´

BamHI-HF

% Activity 100 50 10 100

#R3136S 10,000 units . . . . .¥ 6,000 #R3136L 50,000 units . . . .¥ 24,000

਒༏๱࿚Ī5Xī

#R3136T 10,000 units . . . . .¥ 6,000 #R3136M 50,000 units . . . .¥ 24,000

ʹʺćpUC19 ɞȷɈ഼ɈʯˁʑʷʡDNAɬಜ

ඣȳɥ௲ਗĂ5 unitsɈਊ೧ȦྼᅟɁȜɥă

BamHI-HFɈDNAಜඣငڮɅൎȳɥູڽ౯Ȧ৐

௫ȱɀȞɥȹɛĂ๝࡜܋຦ෂɅȤȞɀɜਊ೧ɂ

DNAȦࣅȩय़ਗȳɥȭɂȦȜɥă๝࡜܋຦௫Ɉ

ʨˋʡȦʑʹɺɅɄɥ௲ਗĂ܋຦೐Ʌ୒༏๱0.1

- 0.5%ɈSDSɬ๒ݡȳɥă

▼

▲

5 ´

. . .

G G A T C C

. . .

3 ´

. . .

C C T A G G

. . .

5 ´

BanI

% Activity 10 25 <10 100

#R0118S 5,000 units . . . .¥ 11,000 #R0118L 25,000 units . . . .¥ 44,000

ʹʙ˃ݛࠐି౯ćdcmʹʙ˃ݛɂʂĜʨĜˁʛ ʯȳɥ௲ਗɅɉʮ˅ʛʇȯɦɥă፪໳ᇆʊʦʸ

DNAɈಜඣɉĂCpGʹʙ˃ݛȦʂĜʨĜˁʛʯ

ʹʺćཱܾ૪࠰ȦෳȞĂਊ೧༏๱Ȧ਒ȞĂʈ˂

ʓ˅Ĝ˃༏๱Ȧ> 5%Ɉ௲ਗĂʑʗĜߤ౯ɬ౺Ȳ

ɥȭɂȦȜɥă

5 ´

. . .

G G Y R C C

. . .

3 ´

. . .

C C R Y G G

. . .

5 ´

▼

▲

BanII

% Activity 100 100 50 100

#R0119S 2,000 units . . . . .¥ 8,500 #R0119L 10,000 units . . . .¥ 34,000

ʹʺćཱܾ૪࠰ȦෳȞ௲ਗĂʑʗĜߤ౯ɬ౺Ȳ

ɥȭɂȦȜɥă

5 ´

. . .

G R G C Y C

. . .

3 ´

. . .

C Y C G R G

. . .

5 ´

▼

▲

制限酵素

(12)

BbsI

NEBuffer 1.1 2.1 3.1 CutSmart % Activity 100 100 25 75

#R0539S 300 units . . . .¥ 11,000 #R0539L 1,500 units . . . .¥ 44,000

ູෳćTime-Saver

ʹʺćBbsIɉBbvIIɈɼʕʏʖʶĜɁȜɥă

30໲ڬ௫Ɉ။ശɬȳɥ௲ਗĂ–70ŎɁ။ശȳɥă

5 ´

. . .

G A A G A C (N)

₂

. . .

3 ´

. . .

C T T C T G (N)

₆

. . .

5 ´

▼

▲

BbvI

% Activity 100 100 25 100

#R0173S 300 units . . . .¥ 11,000 #R0173L 1,500 units . . . .¥ 44,000

ཱܾ௸ॣć CutSmart BufferĂ37Ŏ

ʹʙ˃ݛࠐି౯ćdamĂdcmĂɘȹɉ፪໳ᇆɈ

ʹʺćಜඣȱɀ4ܶࡊɈ5Ōໃ୸Ⴢ඘ɬ౺౰ȳɥă

౺ȲɥȭɂȦȜɥă

5 ´

. . .

G C A G C (N)

8

. . .

3 ´

. . .

C G T C G (N)

12▲

. . .

5 ´

▼

BbvCI

% Activity 10 100 50 100

#R0601S 100 units . . . ._{¥ 10,500} #R0601L 500 units . . . ._{¥ 42,000}

ɥȭɂȦȜɥă

5 ´

. . .

C C T C A G C

. . .

3 ´

. . .

G G A G T C G

. . .

5 ´

▲

▼

BccI

% Activity 100 50 10 100

#R0704S 1,000 units . . . .¥ 11,000 #R0704L 5,000 units . . . .¥ 44,000

౺ȲɥȭɂȦȜɥă

5 ´

. . .

C C A T C (N)

₄

. . .

3 ´

. . .

G G T A G (N)

₅

. . .

5 ´

▼

▲

BceAI

% Activity 100 100 100 100

#R0623S 50 units . . . .¥ 10,500 #R0623L 250 units . . . .¥ 42,000

ʹ ʙ ˃ ݛ ࠐ ି ౯ć፪ ໳ ᇆ ʊ ʦ ʸDNAɈ ಜ ඣ ɉ

CpGʹʙ˃ݛɅɢȽɀʮ˅ʛʇȯɦɥăĪp315

ɬઞ௎īă

ɥȭɂȦȜɥă

5 ´

. . .

A C G G C (N)

₁₂

. . .

3 ´

. . .

T G C C G (N)

₁₄

. . .

▼

5 ´

▼

BcgI

% Activity 10 75* 100 50*

#R0545S 250 units . . . ._{¥ 11,000} #R0545L 1,250 units . . . ._{¥ 44,000}

ཱܾ௸ॣćNEBuffer 3.1 + SAMĂ37Ŏ

ʹʙ˃ݛࠐି౯ćdamʹʙ˃ݛȦʂĜʨĜˁʛ

ʯȳɥ௲ਗɅ܆ࣗɬିȫɥă፪໳ᇆʊʦʸDNA

ɈಜඣɉĂCpGʹʙ˃ݛȦʂĜʨĜˁʛʯȳɥ

௲ਗɅʮ˅ʛʇȯɦɥĪp315ɬઞ௎īă

ʹʺćBcgIɉDNAɬ2ޑಜඣȱɀ໻ૻငڮɬಜ

ɤ୸ȳȭɂɁĂ2ܶࡊɈ3Ōໃ୸Ⴢ඘ɬ૩Ⱦ32ܶ

ࡊൎɈඣ၀ɬ౺౰ȳɥă

ཱܾɅɉS-adenosylmethionineĪਊ೧Ʌ࿥മī

Ȧྼᅟă

* NEBuffer 2.1ȤɢɍCutSmart Bufferɬ઴ᅙȱȹ௲ਗĂ ʑʗĜߤ౯ɬ౺ȲɥȭɂȦȜɥă

5´. . .

10(N) C G A (N)6T G C (N)12. . .3´ 3´. . .

12(N) G C T (N)6A C G (N)10. . .5´ ▼

▲

▼

▲ 2X

増量

制限酵素

(13)

BciVI

% Activity 100 25 <10 100

#R0596S 200 units . . . .¥ 10,500 #R0596L 1,000 units . . . .¥ 42,000

ʹʺćBciVIɉĂိߦჂ඘ඣ၀ɢɤˁɼʊĜʏʿˋ

౺౰ȳɥă

5 ´

. . .

G T A T C C (N)

₆

. . .

3 ´

. . .

C A T A G G (N)

₅

. . .

5 ´

▼

▲

BclI

% ActivityNEBuffer 501.1 1002.1 1003.1 CutSmart75

#R0160S 3,000 units . . . . .¥ 9,500 #R0160L 15,000 units . . . .¥ 38,000

ʹ ʙ ˃ ݛ ࠐ ି ౯ćdamɈ ʹ ʙ ˃ ݛ Ʌ ɢ Ƚ ɀ ʮ

ʹʺćBclIɈಜඣɅɢɤ౺Ȳɥ5ŌGATCໃ୸Ⴢ඘

ɉĂBamHIĂBglIIĂMboIĂSau3AIȤ ɢ ɍBstYI

ɈಜඣɅɢɤ౺ȲȹDNAඣ၀ɂˁɼʊĜʏʿˋ

Ɂȧɥă

5 ´

. . .

T G A T C A

. . .

3 ´

. . .

A C T A G T

. . .

5 ´

▼

▲

BcoDI

% Activity 50 75 75 100

#R0542S 1,000 units . . . .¥ 11,000 #R0542L 5,000 units . . . .¥ 44,000

CpGʹʙ˃ݛȦʂĜʨĜˁʛʯȳɥ௲ਗɅʮ

ʹʺćBcoDIɉBsmAIɈɼʕʏʖʶĜɁȜɥă

5 ´

. . .

G T C T C (N)

₁

. . .

3 ´

. . .

C A G A G (N)

₅

. . .

5 ´

▼

▲

BfaI

% Activity <10 10 <10 100

#R0568S 500 units . . . . .¥ 9,000 #R0568L 2,500 units . . . .¥ 36,000

ູෳćCutSmart

ʹʺćBfaIɉMaeIȤɢɍRmaIɈɼʕʏʖʶĜɁ

Ȝɥă

30໲ڬ௫။ശȳɥ௲ਗĂ–70ŎɁ။ശȳɥă

5 ´

. . .

C T A G

. . .

3 ´

. . .

G A T C

. . .

5 ´

▼

▲

BfuAI

NEBuffer% Activity <101.1 2.125 1003.1 CutSmart10

#R0701S 250 units . . . .¥ 10,500 #R0701L 1,250 units . . . .¥ 42,000

ູෳćTime-Saver

ʹ ʺćBfuAIɉBspMIɈ ɼ ʕ ʏ ʖ ʶ Ĝ Ɂ Ȝ ɥă

BfuAIɉBspMIɢɤৌᆎโɅʯˁʑʷʡDNAɬಜ

ඣȳɥă

BfuAIɈಜඣɅɉ2ȥஒɈ໻ૻ༯ᇙɉྼᅟɁȜɥă

౺ȲɥȭɂȦȜɥă

▲

5 ´

. . .

A C C T G C (N)

₄

. . .

3 ´

. . .

T G G A C G (N)

₈

. . .

5 ´

▼

BfuCI

% Activity 100 50 25 100

#R0636S 400 units . . . .¥ 10,000 #R0636L 2,000 units . . . .¥ 40,000

ູෳćCutSmartĂTime-Saver

CpGʹʙ˃ݛɂʂĜʨĜˁʛʯȳɥ௲ਗɅʮ

ʹʺćBfuCIɉSau3AIɈɼʕʏʖʶĜɁȜɥă

5 ´

. . .

G A T C

. . .

3 ´

. . .

C T A G

. . .

5 ´

▼

▲

制限酵素

(14)

BglI

#R0143S 2,000 units . . . . .¥ 9,500 #R0143L 10,000 units . . . .¥ 38,000

CpGʹʙ˃ݛȦʂĜʨĜˁʛʯȳɥ௲ਗɅʮ

ʹʺćBglIಜඣɅɢȽɀ౺౰ȯɦɥໃ୸Ⴢ඘ɉĂ

ʯˁʑʷʡȤɢɍʭɹĜʐʊʦʸɈ੔৭౰Ăɘȹ

ᄘ౺ळɂဿڽळɈDNAඣ၀Ɉ೨ࠓȢɅ઴ᅙɁȧ

ɥă(Burger, K. J. and Schinzel, R., (1983)

Mol. Gen., 189, 269 - 274)

5´. . ._{G C C N N N N N G G C}. . .₃´

3´. . ._{C G G N N N N N C C G}. . .₅´ ▼

▲

BglII

% Activity 10 10 100 <10

#R0144S 2,000 units . . . . .¥ 7,000 #R0144L 10,000 units . . . .¥ 28,000

਒༏๱࿚Ī5Xī

#R0144M 10,000 units . . . .¥ 28,000

5 ´

. . .

A G A T C T

. . .

3 ´

. . .

T C T A G A

. . .

5 ´

▼

▲

BlpI

% Activity 50 100 10 100

#R0585S 500 units . . . .¥ 12,000 #R0585L 2,500 units . . . .¥ 48,000

ʹʺćBlpIɉBpu1102IȤɢɍEspIɈ ɼʕʏʖ

ʶĜɁȜɥă

5 ´

. . .

G C T N A G C

. . .

3 ´

. . .

C G A N T C G

. . .

5 ´

▼

▲

BmgBI

% ActivityNEBuffer <101.1 2.110 1003.1 CutSmart10

#R0628S 500 units . . . .¥ 10,500 #R0628L 2,500 units . . . .¥ 42,000

ઞ௎īă

ʹ ʺćBmgBIɉBtrIɈ ɼ ʕ ʏ ʖ ʶ Ĝ Ɂ Ȝ ɥă

DNAɬBmgBIɁಜඣȱɀˁɼʊĜʏʿˋȱȹ௲

ਗĂ໻ૻ༯ᇙȦྥʩ˂ˋʡ˅Ĝʸ༯ᇙɁȜɥȹ

ɛĂ੔৭භȯɦȹDNA ༯ᇙෂɅɉ50œɈ໻ૻ༯

ᇙȱȥശ੭ȱɄȞă

౺ȲɥȭɂȦȜɥă

5 ´

. . .

C A C G T C

. . .

3 ´

. . .

G T G C A G

. . .

5 ´

▼

▲

BmrI

NEBuffer 1.1 2.1 3.1 CutSmart

% Activity 75 100 75 100*

#R0600S 100 units . . . .¥ 10,500 #R0600L 500 units . . . .¥ 42,000

ʹʺćBmrIɉĂိߦჂ඘ඣ၀ɢɤˁɼʊĜʏʿˋ

౺౰ȳɥăʂĜʨĜʢɼʠɈཱܾɉైயȱɀȞ

ɄȞăBmrIɉMg2+ ɼʂˋɈᄵჟɅ࠲ɩɣȴູڽ

โɅDNAɬಜඣȳɥȹɛĂEDTAɈശ੭ݚɁɜߤ

౯ɬ૲ȳă

* CutSmart Bufferɬ઴ᅙȱȹ௲ਗĂʑʗĜߤ౯ɬ౺Ȳɥ ȭɂȦȜɥă

5 ´

. . .

A C T G G G (N)

₅

. . .

3 ´

. . .

T G A C C C (N)

₄

. . .

5 ´

▼

▲

2X

増量

制限酵素

(15)

BmtI

#R0658S 300 units . . . .¥ 10,500 #R0658L 1,500 units . . . .¥ 42,000

ʹʺćBmtIɉNheIɈʥʂʏʖʶĜɁȜɥă

5 ´

. . .

G C T A G C

. . .

3 ´

. . .

C G A T C G

. . .

5 ´

▼

▲

BmtI-HF

% Activity 50 100 10 100

#R3658S 300 units . . . .¥ 10,500 #R3658L 1,500 units . . . .¥ 42,000

5 ´

. . .

G C T A G C

. . .

3 ´

. . .

C G A T C G

. . .

5 ´

▲

▼

BpmI

% Activity 75 100 100 100

#R0565S 100 units . . . .¥ 10,500 #R0565L 500 units . . . .¥ 42,000

ʹ ʺćBpmIɉGsuIɈ ɼ ʕ ʏ ʖ ʶ Ĝ Ɂ Ȝ ɥă

BpmIɉထౘɈಜඣငڮɬᄵȳɥࡊଋɅൎȳɥཱ

ܾ౯Ȧ਒Ȟă

5 ´

. . .

C T G G A G (N)

₁₆

. . .

3 ´

. . .

G A C C T C (N)

₁₄

. . .

5 ´

▼

▲

Bpu10I

% Activity 10 25 100 25

#R0649S 200 units . . . .¥ 11,000 #R0649L 1,000 units . . . .¥ 44,000

ʹʺćʈ˂ʓ˅Ĝ˃༏๱Ȧ> 5%Ɉ௲ਗĂʑʗĜ

ߤ౯ɬ౺ȲɥȭɂȦȜɥă

5 ´

. . .

C C T N A G C

. . .

3 ´

. . .

G G A N T C G

. . .

5 ´

▼

▲

BpuEI

% Activity 50* 100 50* 100

#R0633S 500 units . . . ._{¥ 11,000} #R0633L 2,500 units . . . ._{¥ 44,000}

ʹʺć80 mM SAMɈൣɩɤɅ10 mM sinefungin

ɬݡȢɦɊĂ˂ʃʛʠৌᆎɉ95%ڬ௫ɬ૲ȳă

* NEBuffer 1.1ȤɢɍNEBuffer 3.1ɬ઴ᅙȱȹ௲ਗĂʑ ʗĜߤ౯ɬ౺ȲɥȭɂȦȜɥă

5 ´

. . .

C T T G A G (N)

₁₆

. . .

3 ´

. . .

G A A C T C (N)

₁₄

. . .

5 ´

▼

▲

BsaI

% Activity 75 75 100 100

#R0535S 1,000 units . . . ._{¥ 11,000} #R0535L 5,000 units . . . ._{¥ 44,000}

ʹʙ˃ݛࠐି౯ćdcmʹʙ˃ݛɂʂĜʨĜˁʛ ʯ ȳ ɥ ௲ ਗ Ʌ ʮ ˅ ʛ ʇ ȯ ɦ ɥă ፪ ໳ ᇆ ʊ ʦ ʸ

DNAɈಜඣɉĂCpGʹʙ˃ݛȦʂĜʨĜˁʛʯ

ʹʺćBSAɬ๒ݡȳɥȭɂɅɢȽɀ37ŎɁɜ઴

ᅙɁȧɥă50ŎɅȤȫɥߤ౯ɉ100%ɁȜɥă

౺ȲɥȭɂȦȜɥă

5 ´

. . .

G G T C T C (N)

₁

. . .

3 ´

. . .

C C A G A G (N)

₅

. . .

5 ´

▼

▲

1.7X

増量

制限酵素