expression
Vector only
（Wild type)
day0 day2 day4 day6 day9
Fig.10 Comparison of ASCs differentiation to adipocytes. Comparison of adipocyte differentiation between the #5 ASCs
infected lentivirus transfected vector only and deleted NICD overexpression with lentivirus in #5 ASCs.
0 5 10 15 20 25
day0 day2 day4 day6 day9
Vector NICD
R elat iv e m R N A ex pres s ion
Fig.11 Relative mRNA expression of NICD gene in ASCs sample. The relative expression values are depicted as the mean ± standard deviation of three biological replicates and correspond to the ratio between vector only(■) NICD overexpression (▧）
for each day point.
Gene Name Gene Symbol Net intensity {Ratio}
inhibitor of DNA binding 2 Id2 0.46
aldehyde dehydrogenase family 6, subfamily A1 Aldh6a1 0.32
ERO1-like (S. cerevisiae) // ERO1-like (S. cerevisiae) Ero1l // Ero1l 0.38 coiled-coil domain containing 85B // coiled-coil domain
containing 85B
Ccdc85b //
Ccdc85b 0.30
jagged 1 Jag1 0.47
transforming growth factor, beta 1 Tgfb1 0.46
Table.1 DNA Microarray：2DOWN
30 謝辞
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最後に、大学院生活に理解を示してくれた家族、3 人の子供たち まりあ、龍生、莞爾に 深い感謝の意を表し、謝辞といたします。
31 学位(博士)論文の内容に関連する公表論文
・Suzuki A*, Saeki T, Ikuji H, Uchida C and Uchida T (2016) Brown Algae polyphenol, a prolyl isomerase Pin1 inhibitor, prevents obesity by inhibiting the differentiation of stem cells into adipocytes.
PLOS One 11(12) e0168830 (査読有)
・Suzuki A*, Kamo Y, Uchida C, Tanemura K, Hara K and Uchida T (2018) Prolyl isomerase Pin1 is required sperm production by promoting mitosis progression of spermatogonial stem cells.
Biochem.Biophys.Res.Commun.497:388-393（査読有）
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