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v”Œm|%J˃ÛDJ˸D-2DĥAHKJwj”’8˹ņĵÐɣNǀ Ɛ%Jħĕ1˜ƌŠ7ɊɈŬɀǼƽʷǺņĵɪȇĠ2ũĵœ˛2NʸɅ%J D71J 7jS|7ȍģ18˹ɊɈŬ7ƽʷǺ5G-0ņĵ7Ðɣ–

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ǿ7ũÐɦũȱĕRCM1ʏĻ"KJņĵ7ũÐɦȍģ5˒%Jȟȩ8>2

O34"K0H&˹ɷɘ7ȝJ˖I [email protected]ęĆAHKJ7A1 J Liu5GJ1970Œ [84] G:1985Œ [85] 7ȍȃıȓęĆ18˹ņ ĵũÐɦ7ɊɈŬɛöȍģ8dzȎŬģî7¿Ŧǂʭ2#0Ȅ$*ɚɰɃɑ˷œ ɱ4ɊɈɰɁɞ˹ƒ]zP”d˹ɃɑȂ˹Qtl`zɁɞ˹’wȂ“Šʤ Ɂɞ˹īœȂ437dzȎŬɁɞǜǯ4H:5ɊɈɰɁɞ7ǜǯ“ğlj2ɐɁ4 ɊɈŬɅĀɃɑ7ğȄ˸H4I˹Ɵɂȓ58ɧ÷ɊɈNž´2#*ɊɈŬɅ ĀɃɑ5G-0>?IJÎ5ɓƇ"KJ@*˹1995Œ7StalisH [130] 7ȍ ȃıȓęĆ18˹1986Œ1992Œ5ĴƓ#*ǿ7åƹ¹1,472¹7,˹62

¹7ņĵũÐɦ5ɊɈŬɛöȍģNʌ+#0I˹ KHNdzȎŬģî7Ơ ǵ5G-0˹ũÐɦũȱdz˷endomyocarditis̀EMC˸2ũÐɦũȱɊɈ î˷left ventricular endomyocardial fibrosis̀EMF˸25Û0Jäɘ 8ȧ7ȥŘ7dzȎŬɁɞ˷īœȂ˹ƒ]zP”d˹ɃɑȂ˹’wȂ“Š ʤɁɞ43˸7ǜǯ4H:5ɊɈɰɁɞ5ǜǯ“ğlj2ƢǷ4ɊɈŬɅĀɃɑ H4I˹Ţɘ8ųǷ#*ɊɈŬɅĀɃɑH4-0J@*˹EMC8njʰ ȓɱ˶7ǿ˷Lj£ƛőĔŒ˶˿3.4 LJ˸5ĥAHKJ75ļ#0˹EMF8(

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œˑȓ4ȍģNƂ.À´DİĒ#0J 2H˹EMC2EMF8(K)Kā

$Ȍŭ7Ȋ4J•ŠŲ1˹EMC2EMF8&KDā•Ȍŭ˷ũÐɦũȱĕ RCM˸7Ȋ4-*ƛȘNʄȁ#0JD72Ʌʝ©0J˷Ƈʑ%K9˹EMF 8EMC7ɂƣÈ5Șş%J˸ [130](7Ţ˹ Fox [33] 8ũÐɦũȱĕRCM 7ɩŕÈG:ȍȃÈN´ȼȓ5üI@2C*˹ƤȌŭ7ȍȃɃɑıȓǻŨ8 ȠįƾĘʤ˹ɊɈŬɅĀɃɑ˹ɚɰɃɑ7˾.H4JņĵũÐɦ7ˮɷ4Ɋ ɈŬɛö1I˹ũÐɦȍģ8ž5ȠįƾĘʤ2ŞŬɊɈN@$*ɊɈŬɅ ĀɃɑ˷ƛ5ʬ˲îȄN¯˸Hƽų"K˹(7ȗ—5²ɓ%JũÐɦ—ũ

ȱŃ7ȍģ8ñƴɁɞ7ǜǯN¯ɚɰɃɑ7ŠųH4J2#0J ǿ7ũÐɦũȱĕ RCM 7ȍčJ8ȍȃȑȄ5.08@-*ƘH 5"K04˹ʻø7Ȏ¹ęĆEȍȃıȓęĆ18.7¬ʛȣ"

K0J˼.7ʛ8˹xq7ũÐɦũȱĕRCM7•č2"K0JīˆȂğ ĥȎ5¯zŒ”ũȱdz˷Löffler’s myocarditis˸˹ǿ7ũÐɦũȱĕRCM 7ȍč5D4IJ2%JD71J [33] K8ʻø7Ȏ¹ęĆ50˹

īˆȂğĥȎ2ʕƐ"K*ǿ7ņĵũÐɦ5ũÐɦũȱĕ RCM 1AHKJG 4ɊɈŬɛöʚCHK* 25Ę/0J [98˹126˹129]##4 H˹ KH7ęĆ1Ÿ-0JȎ¹8&KDƊȑ¹1J 2H˹(7č Ƭ˒¼5.08¥72 LƘH5"K04 [33˹93]D˼.8˹Ƥ Ȍŭ7ǻŨȓȍģ1Jņĵ7ũÐɦG:˻J8ũÐɦ—ũȱŃ7ȍȓ ģî˹TSŽeŬũȱdzJ8ɪňÌȋŬũȱdz7¿Ŧǂʭ5ʧč%JD 72%Jʛ1J [33] 7¬ʛ8˹ʻø7ȍȃıȓƹɀ50ũÐɦũȱ Ń5÷č˜Ƙ7dzȎŬǂʭAHK0* 2˹Meurs H [101] ĴƓ#*

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TSŽe_u…7İĒũȱdz7ȑȄ2ȗɅ#0*38ƘH5"K 04 [21˹33˹71]4˹ũȱƸĜEũȱ˜Î5GIˈȷî#*HCM J8¨7ȍĕ7ũȱȎ7ƣơÈ1JýɠŬDȣĉ"K0J˹ KH5

˒#0DƆǤ7ėNÚ4 [21]

7G5˹ K@17ęĆ18ƤȌŭ5ǻŨȓ1J2"KJņĵũÐɦ ȍģ5zW”YeNş0*ȍȃɃɑıȓƹɀ4"K08JD77˹ʤȓ J8ˊȓ5Ū&#DïÛ184˹(7ȍȃȑȄ5.08ÎƘH5"

K042-0Dʻʑ184( 1Ƥȭ18˹ȯ˼ȭ1üIŸ-*ũ ÐɦũȱĕRCMɔŭǿ41¹7ũɨNȆ0ņĵ7ũÐɦȍģʙɁ5ƹɀ#˹

ȍȃȑȄ5.0DƹʓNèJ 22#*

ƥ

ƥƏ 2 ƒǔ 1. ũɨ7ɚșȓG:Ƀɑıȓƹɀ

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åƹ8&KDLjŢ12ƛˑ«Ð5ĴƓ#*ũɨ2ɜNɟɣÐGI•Ɓ#0ü IÚ#˹10%œŬɌʃ€Žƒ’Ǩǝœ5!2ǜǮ˹5Ɩˑ«–Đij#*Ţ˹

ɚșȓƹɀ5º#*ũɨ7ɚșȓƹɀG:ÜIÚ#8«—7Ŷ˧1ĴƓ#

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ď17 ũÐɦũȱĕRCM5ɔŭ#*ũɨ7Ĥʏ˷No. 38˹ˡȧ˹16LJ˹ø ìǠA˞˸.˷A˸äĠÄ˹˷B˸ŢĠÄ. ȖȕI = 1 mm.

A B

ď18 ũɨ7ÜIÚ#ƒǔ˷No. 38˹ˡȧ˹16LJ˹øìǠA˞˸

˷–NJ˸ũɨɍƐ˦. ņĵũÐɦ7>?Îėˮɷ5ɛö#0J. þ: ä ĠÄ˹ņ; ŢĠÄ. ȖȕI = 1 mm.

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22. ņĵũÐɦȍģ7ÌȋɃɑîıȓƹɀ

ņĵũÐɦȍģÐ5İĒ%JɁɞ7ȇƨNāij%J*C5˹ÿȧź´NȆ

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zR’íĖ{m]H¶ʇ#*ö"Ƚ6 µm7ɺÜǹ5˹Qyd’“yXl

’“ŽX[ck”hǔ˷VectastaiǹVector laboratories̀Burlingame˹ California˹USA˸NʾȆ#* [7]•DŽź´58˹źƒTevimentin‡u]

”sŽź´˷ˑɶȼɁɞƒ”Y”̀]”’Vim 3B4̀100Áŋˇ̀Ʃ¤˹

k`“dˆw’˸˹źxqα-smooth muscle actin˷α-SMA˸“ƒTe‡u]”

sŽź´ ˷őǫȱɁɞƒ”Y”̀]”’1A4̀100Áŋˇ̀Ʃ¤˹k`“d

ˆw’˸˹źƒTecaldesmon‡u]”sŽź´˷őǫȱɁɞƒ”Y”̀]

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ck”h˜ǘî×ȃNƓ#*̀ pH 7.47]V’ˆɌʃǝ˷PBS˸13Ûˑ“

3ČǗǚ#*Ţ˹10%‰\džōʀǟNɃɑǹ7ħ"5ū$0eŒSr1ƫ*

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—˹ÒŘPBS13Ûˑ“3ČǗǚ#*̀ DŽ1˹˅Ⱦʗɻ2#0ŽX[c k”hƿʟeq|qQyd’NÿeŒSr523ǬǬ—#˹PBS13Ûˑ“

3ČǗǚŢ˹dQ„u~’dd’ǍǨǝ˷DAB⁺“Liquid˹k`“dˆw’˹Ʃ

¤˸N23ǬǬ—#0˹1Ûˑȑɮ"'*̀ ƟŢ5ɸȉǍ1Ǘǚ#˹ƒS

‰”7}ƒq[c’12ÛˑļnjƮɮ#*

33. ÛįȄǺıȓƹɀ

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real-time PCRNĴƓ#*

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ŽeTSŽe˷FHV˸˹ǿǎȒʀȂǡĿȎTSŽe˷FPLV˸˹ǿ`sTS

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143]FCoV G: FCV 7|ŒSƒ”gmq8(K)K7¾İ˨ė˷FCoV˿ 7bj’w]`”r˨ė̀FCV˿5’ƣȮ7˥ɖʔ˨ė˸Nj”_mqˀ®į2#

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22) DNA G: RNA a’|Ž7żÚ

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3) real-time PCR

ƤĴ˱181Č7real-time PCRʐƪ1DNATSŽeG:RNATSŽe

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DNAa’|ŽȆ RNAa’|ŽȆ

DNAa’|Ž RNAa’|Ž

A

DNAa’|ŽȆ RNAa’|ŽȆ B

DNAa’|Ž RNAa’|Ž

C

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0.4 µl˹Nuclease-free water 3.2 µl˸˹DNAJ8RNAa’|Ž 5 µl˹

|ŒSƒ”„m]e 1 µl˷|ŒSƒ”G:|”{N50 µM&.ĄƠ%JG 5ʜƌ˸OS-RT-qPCR 588ʸlŠ”{˷LightCycler^® 8-Tube Strips˹ diagnostics˹Mannheim˹Deutschland˸N¸Ȇ#˹ņȮH˧5FHV˹FPLV G:ACTB7úūǨǝ˷DNAa’|ŽN¸Ȇ˸˹DŽ1FCV˹FCoV˹FIV˹ FeLVG:ACTB7úūǨǝ˷RNAa’|ŽN¸Ȇ˸˷ʒ20 µl˸NȘ¢ǏƮ

#4G5Ɂũ7ǖŰNŷ-0Ûǖ#*˷ď20˸@*˹˘Ŭ`’q”Ž8 a’|Ž28à78ʸlŠ”{5˹ƒej”„m]e 14 µl˹Nuclease Free Water 5 µlG:ACTB7|ŒSƒ”„m]e 1 µl NÛǖ#˹a’|Ž2ā ƛ5OS-RT-qPCRNĴƓ#*OS-RT-qPCR7úūƧ­8TsuchiakaH [136]

7ƒǔ5ţ-0«—7ʶI2#*˿ʴʭÓúū - 45“5Ûˑ˹ÞơǸģŬ 95“30Ȥˑ̀aS]’^˷40aS]Ž˸- ǸģŬ95“10Ȥˑ˹Qt”

’^55“20Ȥˑ˹°Ŝúū72“20ȤˑƤĴ˱8Ș¢ǏƮNˁJ*

C5ǽȬ#*˽Č7Ĵ˱Nʁ˹2Č2D_u…7ğŏʚCHK*ĚĀ5˙Ŭ 2ßƐ#*

Ʌ Ʌ Ƭ 1. ɚșȓƹɀ

ũɨ7ɚșȓƹɀɅƬ7ƼʊNʄ35ȣ#*HW822.55.5 g˷őĔƿ ǦÃŇ̀ȵĎ 10.4-34.7 g˸˹HW/BW85.91.2 g/kg˷ȵĎ 2.6-8.7 g/kg˸1˹

&KDdžōǿ˷HW 17.4 g˹ȵĎ 11-23 g̀HW/BW 4.50.2 g/kg˸2nj=0

˳ÂNȣ#0* [13˹63˹86]ũɨ7Ĥʏ5.08˹41¹Î¹1ņŴ G:ņũə7œȰŘˈŘ7ƀħAHK˹(7,12¹18þŴ7œȰŘ ˈŘ7ƀħDAHK*˷Nos. 8˹14˹17˹20˹21˹27˹28˹34˹36 - 40˸˷ď 21˸ũɨ7ɍƐ˦18˹ŠŲ˹ÛŊƾŚEÛŊȵĎ5ʽ8AHKJD77˹

&K7¹50DũÐɦ5ɊɈŬȍģŠų"K0*@*˹ņĵ58 ƽʷȓ‡p’^˷ņĵɪȇĠG:˻J8ũĵœ˛7ʯ؜ȰŘ7 ɛö˹ņĵɪȇĠG:˻J8ũĵœ˛7Łŵȓ4ɴɺî˹ņĵɣ7F AEģŠ˸D˪ɏ5ʏĻ"K*è0˹41¹œ12¹˷29.3%̀Nos. 2, 5, 6, 14, 20, 21, 24, 25, 27, 34, 38, 41˸58ņŴ˹ņũəJ8ņĵÐ5ľĕħĕ7 ĠĒŬʀƲŠų"K0*˷ď22˸

ņĵũÐɦȍģ7ÛŊƾŚ82.5ħà"K*˷šɘ7œˑȓ4ǻŨNƠ#˹

ÛŊƾŚ7ĞȈŒS’˜Ƙț4¹D#9#9AHK*˸D-2DĥAHK

*78˹ 1Ƥ4#8ʈƋƤ7ƷƯǼ7ɊɈŬƽʷǺJ8ŏŔ“ħĕ“˜

ƌŠ1ŌǼ4#8ƎǼ7ɊɈŬƽʷǺ˹ņĵɪȇĠ2ũĵœ˛NʸɅ#0 Jwj”’7D71-*˷ƎǼɛöwj”’˸˷ď23˸ 7jS|7ũÐ ɦȍģ841¹œ34¹5AHK*˷Nos. 2 - 4˹6 - 11˹13 - 16˹18 - 25˹28 - 37˹

39 - 41˸ƎǼɛöwj”’7ũÐɦȍģ8ʶō˹ņĵ7ÐɣNǀƐ#˹ä ˩

ȱ˷APM˸G:˻J8Ţ ˩ȱ˷PPM˸2ũĵœ˛2NǁǢ##0I˹

ď21 ũÐɦũȱĕRCMɔŭũɨ7Ĥʏ.

˷A˸No. 28˷ˡȧ˹3LJ˹ˁĭǠAˠ˸G:˷B˸No. 41˷ˡȧ˹8 LJ˹øìǠA˞˸˺ņŴ7ˮɷ4ƀħʚCHKJ.

˷C˸No. 34˷ˡȧ˹15LJ˹øìǠA˞˸G:˷D˸No. 37˷ˡȧ˹3 LJ˹ˠ˸˺ņŴ7ˈŘƀħ5è0þŴ7œȰŘƀħDʏĻ"KJ. Ȗȕ I = 1 mm.

A B

C D

ď22 ũÐɦũȱĕRCMɔŭũɨ7ɍƐ˦.

˷A˸No. 5˷ˡȧ˹10LJ˹ˁĭǠAˠ˸˺ņŴɣÐ5ħĕ7ȂǼʀƲŠų

"K0J. ȖȕI = 1 mm.

˷B˸No. 24˷ˡȧ˹18LJ˹øìǠA˞˸˺ņĵ ˩ȱ5ʀƲ˷Ȝó˸©Ț

#0J. ȖȕI = 1 mm.

A B

ď23 ũÐɦũȱĕRCMɔŭũɨ˷ƎǼɛöwj”’˸7ɍƐ˦.

No. 25˷ˡȧ˹3LJ˹øìǠA˞˸˺ņĵɣÐ5ä ˩ȱG:Ţ ˩ȱ2 ũĵœ˛7œˑ˃2NʸɅ%Jŏ7ŔŌǼ7ɊɈŬƽʷǺ˷ũÐɦȍģ˸

Šų"K0I˹Ðɣ–—5ÛƐ"K0J. ņĵɪȇĠ58ʯŘ́œ

ȰŘ7ɛö˹ũŀ˃58˖ŁŬ7ũȱ7ɴɺîG:ņĵÐɣ7ģŠʏĻ

"KJ. ȖȕI = 1 mm.

ƾ4~Ž˷–˃˹œˑ˃J8—˃˸1ņĵɣ7ǾľîE˃Ûȓ4ːĜ G:˻J8ģŠND*H#0*˷ď24˹25˸ 7G4ņĵÐʸɅ wj”’83.5ħà"K˹ APMG:PPM2ũĵœ˛27ʸɅ29¹

˷Nos. 2 - 4, 6, 7, 9, 10, 13 - 16, 18, 21 - 23, 25, 28 - 37, 39 - 41˸˹ PPM2ũĵ

œ˛27ʸɅ3¹˷Nos. 11, 20, 24˸˹ APM2ũĵœ˛27ʸɅ2¹˷Nos.

8, 19˸5ʌ+"K*@*˹ƎǼɛöwj”’7•ŠŲ2#0˹ɉȖǼ4

#8ɾɽ7ńǼ7ɊɈŬƽʷǺņĵ7ɪȇĠ2ũĵœ˛2NʸɅ#0JD 77¹˷Nos. 11˹14˹19˹29˹31˹35˹40˸˷ď26˸5ʚCHK*

•ƒ˹njʰȓĿƋ¹18-*˹ũÐɦ7ɊɈŬɛöȍģņĵ7>?Î ėNʼnʱO1Jwj”’DAHK*˷:ǭŬɛöwj”’˸˷ď27˸ 7 jS|7ũÐɦȍģ841¹œ7¹5AHK*˷Nos. 1˹5˹12˹17˹26˹27˹ 38˸:ǭŬɛöwj”’7ũÐɦȍģ8ņĵ7ǙÍʩG:ǙÚʩ7A4H&˹

ÉŎřʆɓ˷ÉŎř˹ɥɀG: ˩ȱ˸E˹#9#9ņŴ7ũÐɦNDʼn ʱO1I˹ņĵķȨ7ʯŘˈŘ7ǡĿ˹ÉŎřʆɓ7ģŠ“FAE˝ƅ Ƀɑ<7ƅȚ“ȐĀNůʧ#0*˷ď28A˸&K7wj”’50D˹

ɛö#*ũÐɦ8ɷ#ȡŘNğ#˹DzȒɮ˴ʉɮ1ʵƘűNĪ-0*

4˹ʄ˦7•˃J82 L3 L5ľĕ1˴ʉɮɲʉɮ7ʀƲ©Ț

#0J¹DAHK*˷ď28B˸

22. Ƀɑıȓƹɀ

ũÐɦȍģ7ĘƤȓ4Ƀɑƽȶ8˹ɚșȓ4wj”’˷ƎǼɛöwj”’D

#8:ǭŬɛöwj”’˸5Ĭµ5MH&ā•1-*%4M,˹41

¹7ũɨ5Šų"K0*ũÐɦȍģ8%=0˹ƾ4ŠŲNȣ%ˑɶȼɁɞ 7ğlj4H:5ɧ÷ɊɈ2ȹǝǼĘʤ2Nȧ7ȥŘ5@$*ɊɈŬɅĀɃ ɑ˷#9#9ȠįƾNą%J˸7ğȄH4I˹(7ʄ˦8ñŃ7ÐȔɁɞ5

ď24 ũÐɦũȱĕRCMɔŭũɨ˷ƎǼɛöwj”’˸7ɍƐ˦.

˷A˸No. 8˷ˡȧ˹4LJ˹ˁĭǠAˠ˸˺Ȓɮ1ŏ7ŔƎǼ7ɊɈŬƽʷǺ ä ˩ȱ2ũĵœ˛–˃2NʸɅ#0J. @*˹ņĵ7Ðɣ8ƀŜ#˹

ũŀ˃˨ė7ņĵĠ8ɴɺî#0J. ȖȕI = 1 mm.

˷B˸No. 34˷ˡȧ˹15LJ˹øìǠA˞˸˺DzȒɮ1ŏ7ŔƷƯǼ7ɊɈŬ ƽʷǺäG:Ţ ˩ȱ2ũĵœ˛7œˑ˃2NʸɅ#˹ņĵ7Ðɣ–

—5ÛƐ"K0J. @*˹ũŀ˃˨ė7ņĵĠ2ũĵœ˛8˃Ûȓ5ɴɺ î#0J. ȖȕI = 1 mm.

A B

ď25 ũÐɦũȱĕRCMɔŭũɨ˷ƎǼɛöwj”’˸7ɍƐ˦.

˷A˸No. 15˷ˡȧ˹8LJ˹˞˸˺DzȒɮ1ƷƯǼ7ɊɈŬƽʷǺäG:

Ţ ˩ȱ2ũĵœ˛œˑ˃NʸɅ#˹ņĵ7Ðɣ–—5ÛƐ"K0J. ȖȕI = 1 mm.

˷B˸No. 16˷ˡȧ˹8LJ˹ˁĭǠAˠ˸˺DzȒɮ7ɊɈŬƽʷǺ5G-0ņ ĵĠ7–˃2ũĵœ˛7–˃2Ŕȵ5ʸɅ"K˹ņĵǙÚʩˈŘ5ǾȪ

#0J. ȖȕI = 1 mm.

A B

ď26 ũÐɦũȱĕRCMɔŭũɨ˷ƎǼɛöwj”’˸7ɍƐ˦.

˷A˸No. 35˷ˡȧ˹2LJ˹ˠ˸˺DzȒɮ1ɀǼ́ƷƯǼ7ɊɈŬƽʷǺŢ  ˩ȱ2ũĵœ˛7œˑ˃́—˃NʸɅ#0J. ȖȕI = 1 mm.

˷B˸No. 40˷ˡȧ˹10LJ˹øìǠA˞˸˺ƷƯǼG:ɉ7ȖǼ˷ɾɽ7ń Ǽ˸7ɊɈŬƽʷǺä ˩ȱ2ũĵœ˛2NŔȵ5ʸɅ#0J. ȖȕI

= 1 mm.

A B

ď27 ũÐɦũȱĕRCMɔŭũɨ˷:ǭŬɛöwj”’˸7ɍƐ˦. No. 12˷ˡȧ˹3LJ˹ˁĭǠAˠ˸˺ņĵ7ũÐɦ>?Îė5M*-0Ɋ ɈŬ5ɛö#0J. @*˹ˈŘ5ƀŜ#*ņŴ7ũÐɦDˮɷ5ɛö#0 J. ȖȕI = 1 mm.

ď28 ũÐɦũȱĕRCMɔŭũɨ˷:ǭŬɛöwj”’˸7ɍƐ˦.

˷A˸No. 17˷ˡȧ˹5LJ˹˞˸˺DzȒɮ1Ùؘƌ4ɊɈŬȍģņĵ7ũ Ðɦ˹ņŴ7ũÐɦG:ÉŎřʆɓ˷ɥɀ˹ ˩ȱG:ÉŎř˸NŔȵ 5ʼnʱO1J. ȖȕI = 1 mm.

˷B˸No. 27˷ˡȧ˹12LJ˹˞˸˺DzȒɮ1Ùؘƌ4ɊɈŬȍģņĵũÐ ɦ7>?ÎėNʋ-0I˹(7ʄ˦58ŧľ4ʀƲĥƋ©Ț#0J.

ȖȕI = 1 mm.

A B

G-0ʅʋ"K0*˷ď29˸ KH7ũÐɦȍģ8(7ƽųʊȾ24Jɧ÷

ɊɈ7ųǷŘ2ÐĒ%JˑɶȼɁɞ7ŠŲG:ƋH3.7jS|5Û˯"

K*˷ď30˸

˼.Ȗ7jS|7ũÐɦȍģ18˹QŽcQ’ˣƮŬ7ʠĺ4ȹǝǼĘʤ˷ˆ ŬȹǝĥȻ˯˸N@$*œɱ.ɐɁ4ɧ÷ɊɈ2!Ŀˊ7ŞŬɊɈNʨ Ě5˹ĥƋ7ȿˍŠ˹ĥȦŠƙɯǼJ8˜ƌŠ7ˑɶȼɁɞÐŃH ǞŃ50ÎŃŬ5ğlj#0*˷ď31˸jS|˼7ũÐɦȍģ841¹œ

3¹˷Nos. 3˹12˹22˸5ʚCHK*–ʲ#*ˑɶȼɁɞ8ľĕœĕ1Ƙ ț4ƴľ´N12À2ĿˊœȰˊ7ɐɁ4#8ŧɁˬȸǼ7]ƒl’N ÍK*œĕħĕ1˯ÑŠõÑŠD#8ƺÑŠ˷ ,Hž´N4%˸ƴ˹

4H:5ɁɞĞȈƘț˜Ƙț4ɞ´NƠ#0* KH7Ɂɞ7ĥ8 ũĵĠ5ǒ-0ˈŃŬ5njʰȓʍãdž#˄Ý#0*˹ǕǼ˹ǣʼnǼ J8˜ʍã4˄ÝNȣ%D7D#9#9ʌ+"K*

˽.Ȗ7jS|˷jS|˽˸18˹ũÐɦȍģNƽų%Jɧ÷ɊɈ7ųǷŘ 4H:5ÐĒ%JˑɶȼɁɞ7ŠŲ2ɁɞƋ59H.AHK˹À´ˑJ 8ā•À´Ð1DvV”c‹’5ĺO1*##4H˹&K7¹ DĘƤȓ54IǻŨȓ42ŃƽʷNƠ#0*˷ď32A˸%4M,˹ǛŃ˃

˷ũÐɦÄ˸18ʠĺ4ˆŬȹǝĥȻ˯N@$*œɱ.ɐɁ4ɧ÷ɊɈ2 Ŀˊ7ŞŬɊɈNʨĚ5ȿˍŠƙɯǼ7ˑɶȼɁɞƗȕ5ğlj#0I˹

jS|˼2B6āƾ7ɃɑÈNą#0*˷ď32B˸4˹ǛŃ˃1ğlj

#0JɁɞ7•˃8˯ÑŠ4#õÑŠ7ŠŲNüI˹qŽSd’ˣƮɮ1 ȊƮŬNȣ%ʬ˲Ęʤ5üIĎ@K0˷ʬ˲ľȫÐ5ĖǑ#0˸*˷ď33˸

•ƒ˹ǞŃ˃˷ũȱÄ˸8ĹĴ5˄Ý#*ɧ÷ɊɈž´N4#0I˹(7 ˑ˜5Ŀˊ.ɐɁ4ŞŬɊɈÍIʱO1*˷ď34˸@*˹ˑɶȼɁɞ

ď29 ņĵũÐɦ7ɊɈŬɛöȍģ7ɃɑÈ˷No. 1˿ˡȧ˹5LJ˹øìǠA˞˸.

˷A˸ũÐɦȍģ8ƾ4ŠŲNȣ%ˑɶȼɁɞ7ğlj2ɊɈŬɅĀɃɑ7ğȄ5G IŠų"K˹(7ʄ˦NñŃ7ÐȔɁɞʅʋ#0J. HEƮɮ. Bar = 100 µm.

˷B˸ũÐɦȍģNƽų%JɊɈŬɅĀɃɑ8ˣɮ5Ʈ@Jɧ÷ɊɈž´N4#0 J. ƒmi’“q]”…Ʈɮ. Bar = 100 µm.

A B

A B C

ď30 ũÐɦȍģ7ɃɑıȓjS|Û˯.

˷A˸No. 12˷ˡȧ˹3LJ˹ˁĭǠAˠ˸˺jS|1˿ʠĺ4ȹǝǼĘʤN@

$*œɱ.ɐɁ4ɧ÷ɊɈNʨĚ5˹ĥƋ7ȿˍŠ˹ĥȦǼƙɯǼ J8˜ƌŠ7ˑɶȼɁɞÎŃŬ7ğlj#0J˺

˷B˸No. 26˷ˡȧ˹5LJ˹ˁĭǠAˠ˸˺jS|2˿ǛŃ˃˷ũÐɦÄ˸1 8ʠĺ4ȹǝǼĘʤN@$*œɱ.ɐɁ4ɧ÷ɊɈNʨĚ5˹ĥƋ7ȿ ˍŠƙɯǼɁɞğlj#˹ǞŃ˃˷ũȱÄ˸8ĹĴ5˄Ý#*ɧ÷ɊɈ

ž´N4#0J˺

˷C˸No. 27˷ˡȧ˹12LJ˹˞˸˺jS|3˿>?ÎėĹĴ4ɧ÷ɊɈƦ H4I˹ĿƋ7ɁˏŠ7ˑɶȼɁɞɧ÷ɊɈƦ7ˏʮ5ǒ-0˄Ý#0 J. Bar = 100 µm.

ď31 ũÐɦȍģ“jS|17ɃɑÈ˷No. 12˿ˡȧ˹3LJ˹ˁĭǠAˠ˸.

˷A˸ũÐɦȍģ8ȿˍŠ́ƙɯǼɁɞ7ğlj2œɱ4ɊɈŬɅĀɃɑ7ğ ȄH4J. ƒmi’“q]”…Ʈɮ. Bar = 100 µm.

˷B˸ɐɁ4ɊɈŬɅĀɃɑ8QŽcQ’ˣ˷pH 2.5˸ƮŬ7ˆŬȹǝĥȻ

˯Nʠĺ5ƈ0J. QŽcQ’ˣƮɮ. Bar = 100 µm.

A B

A B

C

ď32 ũÐɦȍģ“jS|27ɃɑÈ˷No. 26˿ˡȧ˹5LJ˹ˁĭǠAˠ˸.

˷A˸ũÐɦȍģ8ĞȈ˜Ƙț42ŃƽʷNą#0J. ƒmi’“q]”

…Ʈɮ. Bar = 100 µm.

˷B˸ǛŃ˃˷ũÐɦÄ˸8jS|12āƾ5ɐɁ4ɊɈŬɅĀɃɑ2QŽcQ

’ˣƮɮ˙ŬĘʤ˷ľƭ˸NʨĚ5ǘȑ5ğlj%JȿˍŠ́ƙɯǼɁɞH4J.

ƒmi’“q]”…Ʈɮ. Bar = 50 µm.

˷C˸ǞŃ˃˷ũȱŃÄ˸8Ĺ5˄Ý#*ɧ÷ɊɈƦH4I˹(7ˑ˜5Ɂˏ ȿˍŠɁɞĖ@IʱO1J. ƒmi’“q]”…Ʈɮ. Bar = 50 µm.

A B A

A

ď33 ũÐɦȍģ“jS|27ɃɑÈ˷No. 19˿ˡȧ˹9LJ˹˞˸.

˷A˸ƒmi’“q]”…Ʈɮ. Bar = 100 µm.

˷B˸ũÐɦȍģ7ʄŃ˃5İĒ%JɁɞ7•˃˷ďA7˵ƭ˃˸8qŽ

Sd’ˣƮɮ1ȊƮŬNȣ%ʬ˲Ęʤ˷ʬ˲ľȫ˸Ð5ĖǑ#0J. q

ŽSd’ˣƮɮ˷pH. 2.5˸. Bar = 100 µm.

ď34 ũÐɦȍģ“jS|27ɃɑÈ˷No. 1˿ˡȧ˹5LJ˹øìǠA˞˸˺ ǞŃ˃18ĹĴ4ɧ÷ɊɈ5@$-0ƾ4Ĩ"7ŞŬɊɈʏĻ"K J. VŒelY“‘’\”i’Ʈɮ. Bar = 100 µm.

8ɧ÷ɊɈ7ˏʮƒĂ5ǒ-0njʰȓʍãdž#˄Ý#0*˹(7Ƌ8Ǜ Ń˃2nj=0Ŀ4-*˷ď32C˸jS|˽7ũÐɦȍģ841¹œ19¹˷Nos.

1˹7˹9˹13 - 17˹19˹21˹25˹26˹29˹30˹32˹35˹37˹39˹40˸5ʚC HK*

3.Ȗ7jS|˷jS|3˸18˹ɛö#*ũÐɦ7>?ÎėĿˊœȰˊ 7ŞŬɊɈN@$*ĹĴ4ɧ÷ɊɈƦHƽų"K0I˹#9#9Ƙț4 ʬ˲îȄ˷ƛ5ȞDzǐȚ#0J˸N¯-0*˷ď34˸ 7jS|7ũÐ ɦȍģ1D˹jS|27ǞŃ˃2āƾ5˹ɁˏŠ7ˑɶȼɁɞž5ɧ÷Ɋ ɈƦ7ˏʮƒĂ5ǒG5˄Ý#0*˹(7Ƌ8jS|2GID"H5 ǡĿ#0*jS|˾7ũÐɦȍģ819¹˷Nos. 2˹4 - 6˹8˹10˹11˹18˹

20˹23˹24˹27˹28˹31˹33˹34˹36˹38˹41˸1ʏĻ"K*

(K)K7jS|7ũÐɦȍģNƠ#0*ǿ7Lj£ƛőĔŒ˶8˹jS|

˼˿1.81.4LJ˷ȵĎ 0.3 - 3.0LJ˸˹jS|˽˿5.52.6LJ˷ȵĎ 2 - 11LJ˸˹

jS|˾˿10.25.2LJ˷ȵĎ˿3 - 19LJ˸1-*4˹StutPlus^®˷AnalystSoft Inc.˹WALNUT˹CA˹US˸NȆ*Ɇʒ×ȃ˷one-way analysis of variance˸ 18˹ÿjS|7őĔLj£ƛŒ˶7ˑ5ƠŰŇʚCHK*˷P < 0.001˸

DŽ5˹ɚșȓwj”’à˷:ǭŬɛöwj”’2ƎǼɛöwj”’˸5(K )K7ũÐɦG:ũÐɦ—ũȱŃNȺư%J2˹:ǭŬɛöwj”’˷41¹

œ7¹˸18˹ɛö#*ũÐɦ2ũÐɦ—ũȱŃ8>2O37¹1ĞȈM C0Ƙț1I˹µH7dzȎŬǂʭ7˒™Nȣĉ%JG4Ƀɑŵʌ˷dzȎ

ŬɁɞ7ǜǯEũȱ7ģŬ“ġLj“ɿʐJ8ˑʤŬ7ũȱɊɈî˸8-"ʌ+"K4-*##4H˹!ĿƋ¹18ũÐɦ5Ȅ$*ɊɈ ğȄȧ7ȥŘ5ũÐɦ—ũȱŃÐ5@1Ǖù#0I˹ #*˃²18 ũȱɊɈʯ؜ȰŘ5ɵɎ#0*

ď35 ũÐɦȍģ“jS|37ɃɑÈ.

˷A˸No. 27˷ˡȧ˹12LJ˹˞˸˺ɛö#*ũÐɦ7>?ÎėĹĴ4ɧ÷Ɋ ɈƦH4I˹(7ˑ˜5ɁˏȿˍŠɁɞĖ@IʱO1J. ƒmi

’“q]”…Ʈɮ. Bar = 100 µm.

˷B˸No. 38˷ˡȧ˹16LJ˹øìǠA˞˸˺ũÐɦȍģ7•˃5ʬ˲îȄA HKJ˷ľƭ˿qŽSd’ˣƮɮ˸. ƒmi’“q]”…Ʈɮ. Bar = 100 µm.

˷C˸No. 38˷ˡȧ˹16LJ˹øìǠA˞˸˺B2à7˃²18ʬ˲îȄ#*˃

²5ȞDzǐȚN¯-0J˷Ȝó˸. HEƮɮ. Bar = 100 µm

ʬ˲“˲Šų

A B

C

ƎǼɛöwj”’˷41¹œ34¹˸7ũÐɦȍģ18˹&K7¹50 Dœũ˃5²ɓ%J|Ž[’VɊɈƦ2(KNĎɒ%JĹĴ4ɧ÷ɊɈƦH 4J˹MFJ “ņĵ¬ɥɀ˷left ventricular false tendoǹLVFT˸”ȍńÐ 5ĥƋİĒ#0*˷ď36˸(#0˹ȿˍŠƙɯǼ7ˑɶȼɁɞ KH LVTFNʨĚ5āũÑǼ5ğlj%J22D5˹œɱ.ɐɁ4ɊɈŬɅĀɃɑ NƗȕ5ȅȄ%J 21ũÐɦȍģ7˲ɃANŠų#0*%4M,˹LVFT 7ƽųʊȾ1J|Ž[’VɊɈƦNüIĎBɊɈŬɅĀɃɑŃ5ÐĒ#0 Jƕİ7ˑɶȼɁɞ˹āũÑǼ5ǘȑ5ğlj#4HˆŬȹǝĥȻ˯Nʠĺ 5ƈ*ɐɁ4ɧ÷ɊɈNĥˊ5ȅȄ%J 21À7LVFTˮɷ5ğŏ#

0*"H5˹ɛö#*LVFTāĢ¢5ƅȚ“ȐĀ%J 21δ2#

0•ě7ħ4ɊɈŬȍģŠų"K0* 7ȍģŠųʻȥ8–ʲ7j S|15Āɫ%JD71-*˷ď37˸ #*ȍȓǂʭʹʁ%J2LVFT ćĎ7njʰȓœɱ4ɊɈŬɅĀɃɑŃ8˹ɁɞųÛ7ǡĿ22D5GIųǷ#

*ɧ÷ɊɈƦNž´2#*ɊɈŬɅĀɃɑ<2ʭƇ#˷–ʲ7jS|˽5 Șş%Jǂʭ˸˷ď38˸˹"H5ƛˑɄʻ%J2Ɵɂȓ5ȍģ7ÎėɁɞų Û5Ÿ#˹Ĩ0ĹĴ4ɧ÷ɊɈƦ5G-0ɓƇHK0*˷–ʲ7j S|˾5Șş%Jǂʭ˸˷ď39˸4˹ƎǼɛöwj”’7ũÐɦȍģ7Ð

˃E©Ț˃²5DƘH4dzȎŬģî8ʚCHK4-*

33. ÌȋɃɑîıȓƹɀ

ɛö#*ũÐɦ7Ð˃5İĒ#0JȿˍŠƙɯǼɁɞ7>?%=0˹

ˑɶȼɁɞƒ”Y”1Jvimentin˷ď40A˸4H:5őǫȱÛY”1 Jα-smooth muscle actin˷SMA˸5˙ŬúūNȣ#*˷ď40B˸"H5˹

(KHGIDĥĿĿ4G1-*ĥ7ȿˍŠƙɯǼɁɞőǫȱÛ îƒ”Y”1Jcaldesmon5D˙ŬúūNȣ#*˷ď40C˸•ƒ˹ũÐɦ

ď36 ƎǼɛöwj”’Nȣ#*ũ Ðɦȍģ7ɃɑÈ.

˷A˸No. 3˷ˡȧ˹2LJ˹ˁĭǠA ˠ˸: jS|1.

˷B˸No. 9˷ˡȧ˹6LJ˹øìǠA ˠ˸: jS|2.

˷C˸No. 23˷ˡȧ˹9LJ˹øìǠA

˞˸: jS|3˺

&KDɛö#*ũÐɦ7Ð˃58 ņĵ¬ɥɀ˷Ȝó˸ĥƋʌ+"

KJ. ƒmi’“q]”…Ʈɮ˹

Bar = 100 µm.

C

B

A

ď37 ƎǼɛöwj”’Nȣ#*ũÐɦȍģ“jS|17ɃɑÈ

No. 3˷ˡȧ˹2LJ˹ˁĭǠAˠ˸˺ņĵ¬ɥɀ˷Ȝó˸7ƽųÛ1Jȿˍ Š́ƙɯǼɁɞƕİ7|Ž[’VɁɞƦNüIĎBG5āũÑǼ5ğlj

#˹ʠĺ4ȹǝĥȻ˯Nƈ*ɐɁ.ʠĺ4ɧ÷ɊɈNȅȄ#0J˺(

7ɅƬ˹À7¬ɥɀ8ˮɷ5ɛö#˹Ĩ"Nğ#*¬ɥɀāĢ¢5ƅ Ț“ȐĀ%J 21ħĕ7ɊɈŬƽʷǺŠų"K0J. ƒmi’“q

]”…Ʈɮ˹Bar = 100 µm.

A

ď38 ƎǼɛöwj”’Nȣ#*ũÐɦȍģ“jS|27ɃɑÈ

No. 9˷ˡȧ˹6LJ˹øìǠAˠ˸˺ņĵ¬ɥɀ˷Ȝó˸NüIĎO1Jɧ

÷ɊɈ8jS|1GIDųǷ#0J˹ǛŃÄ˷ũĵɣÄ˸18jS|1 2āƾ˹ʠĺ4ˆŬȹǝĥȻ˯Nƈ*œɱ4ɧ÷ɊɈʨĚ5ˑɶȼɁɞ ǘȑ5ğlj#0J. ƒmi’“q]”…Ʈɮ˹Bar = 100 µm.

B

C

ď39 ƎǼɛöwj”’Nȣ#*ũÐɦȍģ“jS|37ɃɑÈ

No. 23˷ˡȧ˹9LJ˹øìǠA˞˸˺ɊɈŬƽʷǺ7>?ÎėɁɞųÛ5Ÿ

#ųǷ#*ɊɈŬɅĀɃɑ5G-0ƽų"K0I˹(7Ð˃5ņĵ¬ɥ ɀ˷Ȝó˸ĖǑ#0J. ƒmi’“q]”…Ʈɮ˹Bar = 100 µm.

ď40 ũÐɦȍģÐ1ğlj%JȿˍŠ́ƙɯǼɁɞ7ÌȋɃɑîıȓƮɮÈ. ȍńÐ7ȿˍŠ́ƙɯǼɁɞ8(7>2O3˷A˸źvimentinG:˷B˸

źa-smooth muscle actinź´5˙ŬúūNȣ#˹(KHGIDɱŐƋ8Ŀ4 4JD77ĥ7Ɂɞ˷C˸źcaldesmonź´5D˙ŬúūNȣ#0J. Bar

= 20 µm.

C B

A

ȍģNƽų%JɊɈŬɅĀɃɑ7Ð˃G:ˑɶȼɁɞ7Ɂɞɦʄ˦58 PDGFG:TGF-βȑȁ#0I˷ď41˸˹(7ȑȁ˪Ř8ƘH5jS|

3GIDjS|1G:jS|25Û˯"K*ũÐɦȍģ1˳-*

44. ÛįȄǺıȓƹɀ

ƤĴ˱5ËȬ,˹¥ČpbS’#*OS-RT-qPCR7TUmqvp”c‹’

2#0˹ÿƹÚļʡ7j”_mqˀ®įNĄB7ȧ˯˷ÿTSŽeG:ACTB˸

7ƕȝDZŘ7ĀųDNA˷IDT5¶ʇ»˫˸Nˌĕ2#*10ÁŋˇȼÝ˷1×10⁰ 1×10⁶ copy/µl˸˹G:¥Č¶ʇ#*|ŒSƒ”gmqNȆ0ƹˊɊN¶ų

#*˷ď42˹43˸(7ɅƬ&K7|ŒSƒ”gmq50D˹ğŏéȀ˹

|ŒSƒ”7űŘ5Ċ˭8AHK4-*

ƤĴ˱2#0ũÐɦũȱĕRCMɔŭǿ7ũɨHżÚ#*DNAa’|Ž

G:RNAa’|ŽNȆ0OS-RT-qPCRNĴƓ#*2 L˹&K7a’|

Ž1D˹ƹÚļʡ2#*6ȧ˯7TSŽe_u…8-"ƹÚ"K4-*

˷ď44~48˸•ƒ˹˙Ŭ`’q”Ž2#0Ȇ*ACTB8%=07a’|

Ž1ƹÚ"K*

A B

ď41 platelet derived growth factor˷PCGF˸G:

transforming growth factor−β˷TGF−β˸5ļ%JÌȋɃɑî ıȓƮɮÈ.

˷A˸PDGFG:˷B˸TGF−β8˹ȍńÐ7ȿˍŠ́ƙɯǼ Ɂɞ7Ɂɞɦʄ˦G:ɊɈŬɅĀɃɑŃ7Ð˃5ȑȁ#0

*. Bar = 20 µm.

ď42 ÿ|ŒSƒ”gmqG:ĀųDNA710ÁŋˇȼÝNȆ0¶ų#*ƹˊɊ

˷FHV, FPLV, FCVG:FCoV˸. CtÂ, ˓Â; E, ğŏéȀ; r², Ș˒¼Ƌ.

ď43 ÿ|ŒSƒ”gmqG:ĀųDNA710ÁŋˇȼÝNȆ0¶ų#*ƹˊɊ

˷FIV, FeLVG:ACTB˸. CtÂ, ˓Â; E, ğŏéȀ; r², Ș˒¼Ƌ.

ď44 OS-RT-qPCR7ɅƬNȣ%^Œz˷ Sample No. 1, 2, 3˸. &K7^Œz1DDNA/

RNAa’|Ž7˙Ŭ`’q”Ž˷ACTB˸8ƹÚ"K0J,

TSŽe_u…8-"ƹÚ"K04.

ď45 OS-RT-qPCR7ɅƬNȣ%^Œz˷ Sample No. 4, 5, 6˸. &K7^Œz1DDNA/

RNAa’|Ž7˙Ŭ`’q”Ž˷ACTB˸8ƹÚ"K0J,

TSŽe_u…8-"ƹÚ"K04.

ď46 OS-RT-qPCR7ɅƬNȣ%^Œz˷ Sample No. 7, 8, 9˸. &K7^Œz1DDNA/

RNAa’|Ž7˙Ŭ`’q”Ž˷ACTB˸8ƹÚ"K0J,

TSŽe_u…8-"ƹÚ"K04.

ď47 OS-RT-qPCR7ɅƬNȣ%^Œz˷ Sample No. 10, 11, 12˸. &K7^Œz1D DNA/ RNAa’|Ž7˙Ŭ`’q”Ž˷ACTB˸8ƹÚ"K0J, TSŽe_u… 8-"ƹÚ"K04.

ď48 OS-RT-qPCR7ɅƬNȣ%^Œz˷Sample No. 13, 14˸. &K7^Œz1DDNA/

RNAa’|Ž7˙Ŭ`’q”Ž˷ACTB˸8ƹÚ"K0J,

TSŽe_u…8-"ƹÚ"K04.

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ドキュメント内 猫の拘束型心筋症（心内膜心筋型）の臨床的および病理学的研究 (ページ 35-165)